BLASTP 2.2.22 [Sep-27-2009] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= batch____ (130 letters) Database: uniref50.fasta 3,077,464 sequences; 1,040,396,356 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value UniRef50_P76549 Uncharacterized protein yffR n=4 Tax=Enterobacte... 266 1e-70 UniRef50_B1IWL2 Putative uncharacterized protein n=1 Tax=Escheri... 62 6e-09 >UniRef50_P76549 Uncharacterized protein yffR n=4 Tax=Enterobacteriaceae RepID=YFFR_ECOLI Length = 130 Score = 266 bits (681), Expect = 1e-70, Method: Compositional matrix adjust. Identities = 130/130 (100%), Positives = 130/130 (100%) Query: 1 MKVLGNILWWAFVGFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGEGAKIMNAAKI 60 MKVLGNILWWAFVGFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGEGAKIMNAAKI Sbjct: 1 MKVLGNILWWAFVGFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGEGAKIMNAAKI 60 Query: 61 MGRDYAWKHVKEDRLPCLFAAGVVADNILVKYHFSNETEFKLKAAAVRECANVAENNGFV 120 MGRDYAWKHVKEDRLPCLFAAGVVADNILVKYHFSNETEFKLKAAAVRECANVAENNGFV Sbjct: 61 MGRDYAWKHVKEDRLPCLFAAGVVADNILVKYHFSNETEFKLKAAAVRECANVAENNGFV 120 Query: 121 LVGRVFEKRN 130 LVGRVFEKRN Sbjct: 121 LVGRVFEKRN 130 >UniRef50_B1IWL2 Putative uncharacterized protein n=1 Tax=Escherichia coli ATCC 8739 RepID=B1IWL2_ECOLC Length = 158 Score = 62.0 bits (149), Expect = 6e-09, Method: Compositional matrix adjust. Identities = 48/140 (34%), Positives = 69/140 (49%), Gaps = 15/140 (10%) Query: 1 MKVLGNILWWAFV-GFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGE--------- 50 M L +L A + GF+ Y K + AV E L +A GE E Sbjct: 1 MNFLDKLLIAAVITGFIVYNEQGKWEEEAAAVQTQHEQLAARVAA-GEKFERTEWVDERT 59 Query: 51 -GAKIMNAAKIMGRDYAWKHVKEDRLPCLFAAGVVADNILVKY---HFSNETEFKLKAAA 106 K+++A I GRD+AW++ + ++ CL AA ADNI VKY S E + K AA Sbjct: 60 TAQKMVDAMGIYGRDFAWENRGKVKINCLEAAERAADNIAVKYSGEEVSGEDVLRGKEAA 119 Query: 107 VRECANVAENNGFVLVGRVF 126 VREC++V N G++L ++ Sbjct: 120 VRECSHVLSNVGYILKDNIY 139 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: UniRef50_P76549 Uncharacterized protein yffR n=4 Tax=Enterobacte... 212 2e-54 UniRef50_B1IWL2 Putative uncharacterized protein n=1 Tax=Escheri... 168 7e-41 Sequences not found previously or not previously below threshold: CONVERGED! >UniRef50_P76549 Uncharacterized protein yffR n=4 Tax=Enterobacteriaceae RepID=YFFR_ECOLI Length = 130 Score = 212 bits (541), Expect = 2e-54, Method: Composition-based stats. Identities = 130/130 (100%), Positives = 130/130 (100%) Query: 1 MKVLGNILWWAFVGFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGEGAKIMNAAKI 60 MKVLGNILWWAFVGFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGEGAKIMNAAKI Sbjct: 1 MKVLGNILWWAFVGFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGEGAKIMNAAKI 60 Query: 61 MGRDYAWKHVKEDRLPCLFAAGVVADNILVKYHFSNETEFKLKAAAVRECANVAENNGFV 120 MGRDYAWKHVKEDRLPCLFAAGVVADNILVKYHFSNETEFKLKAAAVRECANVAENNGFV Sbjct: 61 MGRDYAWKHVKEDRLPCLFAAGVVADNILVKYHFSNETEFKLKAAAVRECANVAENNGFV 120 Query: 121 LVGRVFEKRN 130 LVGRVFEKRN Sbjct: 121 LVGRVFEKRN 130 >UniRef50_B1IWL2 Putative uncharacterized protein n=1 Tax=Escherichia coli ATCC 8739 RepID=B1IWL2_ECOLC Length = 158 Score = 168 bits (425), Expect = 7e-41, Method: Composition-based stats. Identities = 48/142 (33%), Positives = 70/142 (49%), Gaps = 15/142 (10%) Query: 1 MKVLGNILWWAFV-GFMAYATLIKPDNTDPAVVNYEEPLPMELANYGEPGE--------- 50 M L +L A + GF+ Y K + AV E L +A GE E Sbjct: 1 MNFLDKLLIAAVITGFIVYNEQGKWEEEAAAVQTQHEQLAARVAA-GEKFERTEWVDERT 59 Query: 51 -GAKIMNAAKIMGRDYAWKHVKEDRLPCLFAAGVVADNILVKY---HFSNETEFKLKAAA 106 K+++A I GRD+AW++ + ++ CL AA ADNI VKY S E + K AA Sbjct: 60 TAQKMVDAMGIYGRDFAWENRGKVKINCLEAAERAADNIAVKYSGEEVSGEDVLRGKEAA 119 Query: 107 VRECANVAENNGFVLVGRVFEK 128 VREC++V N G++L ++ + Sbjct: 120 VRECSHVLSNVGYILKDNIYYR 141 Database: uniref50.fasta Posted date: Mar 8, 2010 10:38 AM Number of letters in database: 1,040,396,356 Number of sequences in database: 3,077,464 Lambda K H 0.319 0.136 0.371 Lambda K H 0.267 0.0416 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 489,900,567 Number of Sequences: 3077464 Number of extensions: 17252590 Number of successful extensions: 40446 Number of sequences better than 1.0e-01: 2 Number of HSP's better than 0.1 without gapping: 4 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 40438 Number of HSP's gapped (non-prelim): 4 length of query: 130 length of database: 1,040,396,356 effective HSP length: 95 effective length of query: 35 effective length of database: 748,037,276 effective search space: 26181304660 effective search space used: 26181304660 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 87 (38.1 bits)