BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (43 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_C1P5Z7 Putative inhibitor of glucose uptake transporter...    93   3e-18
UniRef50_A4W6H4 Putative uncharacterized protein n=7 Tax=Enterob...    44   0.002

>UniRef50_C1P5Z7 Putative inhibitor of glucose uptake transporter sgrT n=11
          Tax=Escherichia coli RepID=SGRT_ECOLI
          Length = 43

 Score = 92.8 bits (229), Expect = 3e-18,   Method: Compositional matrix adjust.
 Identities = 43/43 (100%), Positives = 43/43 (100%)

Query: 1  MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES 43
          MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES
Sbjct: 1  MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES 43


>UniRef50_A4W6H4 Putative uncharacterized protein n=7 Tax=Enterobacteriaceae
          RepID=A4W6H4_ENT38
          Length = 50

 Score = 43.9 bits (102), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 22/42 (52%), Positives = 25/42 (59%)

Query: 2  RQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES 43
          RQFYQ YF AT  + WL      QRL MLE LMQW+   + S
Sbjct: 7  RQFYQQYFLATKGVSWLARQCAEQRLKMLEELMQWEVTQTTS 48


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_C1P5Z7 Putative inhibitor of glucose uptake transporter...    63   3e-09
UniRef50_A4W6H4 Putative uncharacterized protein n=7 Tax=Enterob...    51   1e-05

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_C1P5Z7 Putative inhibitor of glucose uptake transporter sgrT n=11
          Tax=Escherichia coli RepID=SGRT_ECOLI
          Length = 43

 Score = 62.9 bits (151), Expect = 3e-09,   Method: Composition-based stats.
 Identities = 43/43 (100%), Positives = 43/43 (100%)

Query: 1  MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES 43
          MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES
Sbjct: 1  MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES 43


>UniRef50_A4W6H4 Putative uncharacterized protein n=7 Tax=Enterobacteriaceae
          RepID=A4W6H4_ENT38
          Length = 50

 Score = 50.9 bits (120), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 22/42 (52%), Positives = 25/42 (59%)

Query: 2  RQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES 43
          RQFYQ YF AT  + WL      QRL MLE LMQW+   + S
Sbjct: 7  RQFYQQYFLATKGVSWLARQCAEQRLKMLEELMQWEVTQTTS 48


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.313    0.127    0.409 

Lambda     K      H
   0.267   0.0403    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 175,741,725
Number of Sequences: 3077464
Number of extensions: 3629238
Number of successful extensions: 10193
Number of sequences better than 1.0e-01: 7
Number of HSP's better than  0.1 without gapping: 5
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 10184
Number of HSP's gapped (non-prelim): 10
length of query: 43
length of database: 1,040,396,356
effective HSP length: 17
effective length of query: 26
effective length of database: 988,079,468
effective search space: 25690066168
effective search space used: 25690066168
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.1 bits)
S2: 87 (38.1 bits)