BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (80 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AF47 Uncharacterized protein yjbE n=81 Tax=Enterobact...   142   4e-33
UniRef50_A7MPC6 Putative uncharacterized protein n=2 Tax=Cronoba...    62   7e-09

>UniRef50_P0AF47 Uncharacterized protein yjbE n=81 Tax=Enterobacteriaceae
          RepID=YJBE_ECO57
          Length = 80

 Score =  142 bits (357), Expect = 4e-33,   Method: Compositional matrix adjust.
 Identities = 80/80 (100%), Positives = 80/80 (100%)

Query: 1  MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA 60
          MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA
Sbjct: 1  MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA 60

Query: 61 TGGGDGSNTGTTTTTTTSTQ 80
          TGGGDGSNTGTTTTTTTSTQ
Sbjct: 61 TGGGDGSNTGTTTTTTTSTQ 80


>UniRef50_A7MPC6 Putative uncharacterized protein n=2 Tax=Cronobacter
          RepID=A7MPC6_ENTS8
          Length = 82

 Score = 61.6 bits (148), Expect = 7e-09,   Method: Compositional matrix adjust.
 Identities = 47/60 (78%), Positives = 51/60 (85%)

Query: 1  MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA 60
          MKK+L G+FAI+AL A SA AAPV VGEAAGSAATSVS GSSSAT+ STV S VGVALAA
Sbjct: 1  MKKILSGVFAIAALGAVSAQAAPVSVGEAAGSAATSVSVGSSSATASSTVGSVVGVALAA 60


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AF47 Uncharacterized protein yjbE n=81 Tax=Enterobact...    80   2e-14
UniRef50_A7MPC6 Putative uncharacterized protein n=2 Tax=Cronoba...    46   3e-04

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AF47 Uncharacterized protein yjbE n=81 Tax=Enterobacteriaceae
          RepID=YJBE_ECO57
          Length = 80

 Score = 79.9 bits (195), Expect = 2e-14,   Method: Composition-based stats.
 Identities = 80/80 (100%), Positives = 80/80 (100%)

Query: 1  MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA 60
          MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA
Sbjct: 1  MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA 60

Query: 61 TGGGDGSNTGTTTTTTTSTQ 80
          TGGGDGSNTGTTTTTTTSTQ
Sbjct: 61 TGGGDGSNTGTTTTTTTSTQ 80


>UniRef50_A7MPC6 Putative uncharacterized protein n=2 Tax=Cronobacter
          RepID=A7MPC6_ENTS8
          Length = 82

 Score = 46.0 bits (107), Expect = 3e-04,   Method: Composition-based stats.
 Identities = 47/60 (78%), Positives = 51/60 (85%)

Query: 1  MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSATSVSTVSSAVGVALAA 60
          MKK+L G+FAI+AL A SA AAPV VGEAAGSAATSVS GSSSAT+ STV S VGVALAA
Sbjct: 1  MKKILSGVFAIAALGAVSAQAAPVSVGEAAGSAATSVSVGSSSATASSTVGSVVGVALAA 60


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.306    0.119    0.304 

Lambda     K      H
   0.267   0.0376    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 253,962,977
Number of Sequences: 3077464
Number of extensions: 7675818
Number of successful extensions: 218947
Number of sequences better than 1.0e-01: 96
Number of HSP's better than  0.1 without gapping: 108
Number of HSP's successfully gapped in prelim test: 159
Number of HSP's that attempted gapping in prelim test: 213887
Number of HSP's gapped (non-prelim): 4794
length of query: 80
length of database: 1,040,396,356
effective HSP length: 51
effective length of query: 29
effective length of database: 883,445,692
effective search space: 25619925068
effective search space used: 25619925068
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 43 (22.0 bits)
S2: 87 (38.2 bits)