BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (128 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_B5F8S5 UPF0325 protein yaeH n=172 Tax=Gammaproteobacter...   251   4e-66
UniRef50_Q2NRK3 UPF0325 protein SG1947 n=55 Tax=Gammaproteobacte...   215   4e-55
UniRef50_A1SSV2 UPF0325 protein Ping_0715 n=1 Tax=Psychromonas i...   138   4e-32
UniRef50_Q1ZGX9 Putative uncharacterized protein n=1 Tax=Psychro...   116   2e-25
UniRef50_A6VT88 Putative structural protein n=2 Tax=Marinomonas ...   105   6e-22
UniRef50_A4BBX7 Putative uncharacterized protein n=1 Tax=Reineke...    82   7e-15
UniRef50_Q2BP51 Putative uncharacterized protein n=1 Tax=Neptuni...    62   6e-09

>UniRef50_B5F8S5 UPF0325 protein yaeH n=172 Tax=Gammaproteobacteria RepID=YAEH_SALA4
          Length = 128

 Score =  251 bits (642), Expect = 4e-66,   Method: Compositional matrix adjust.
 Identities = 122/128 (95%), Positives = 127/128 (99%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKD+GEFFAKSVKFKYPRQRKTVVAD
Sbjct: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDRGEFFAKSVKFKYPRQRKTVVAD 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
           G+GQGYKEVQEISPNLRY+IDELDQICQRDRSE+DLKRKILDDLRHLESVV NKISEIEA
Sbjct: 61  GIGQGYKEVQEISPNLRYVIDELDQICQRDRSELDLKRKILDDLRHLESVVANKISEIEA 120

Query: 121 DLEKLTRK 128
           DL+KLTRK
Sbjct: 121 DLDKLTRK 128


>UniRef50_Q2NRK3 UPF0325 protein SG1947 n=55 Tax=Gammaproteobacteria
           RepID=Y1947_SODGM
          Length = 129

 Score =  215 bits (547), Expect = 4e-55,   Method: Compositional matrix adjust.
 Identities = 102/128 (79%), Positives = 118/128 (92%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MYDNLKSLG+ +P++IDRYSLRQEANNDILKIYF++DKGE FAKSVKFKYPRQRKTVV+ 
Sbjct: 1   MYDNLKSLGVNHPDDIDRYSLRQEANNDILKIYFRRDKGELFAKSVKFKYPRQRKTVVSY 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
              +GY+E+  ISPNLRY+IDELDQIC RD++EVDLK KILDDLRHLESVV+NKI+EIEA
Sbjct: 61  NAAEGYREINGISPNLRYVIDELDQICLRDQTEVDLKNKILDDLRHLESVVSNKIAEIEA 120

Query: 121 DLEKLTRK 128
           DLEKLTR+
Sbjct: 121 DLEKLTRQ 128


>UniRef50_A1SSV2 UPF0325 protein Ping_0715 n=1 Tax=Psychromonas ingrahamii 37
           RepID=Y715_PSYIN
          Length = 125

 Score =  138 bits (348), Expect = 4e-32,   Method: Compositional matrix adjust.
 Identities = 65/125 (52%), Positives = 91/125 (72%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MY NLKS+G+ N + ID+Y+LRQE + DILKIYF+K   +F AKS+KFK+PRQRK +  D
Sbjct: 1   MYSNLKSIGVVNYDNIDKYTLRQEGDTDILKIYFRKASRQFLAKSLKFKFPRQRKKISQD 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
               G++ V EI   LRY++ ELD + +  + E +LK ++L+DL+HLESVV +KI EIE 
Sbjct: 61  HGSSGFRNVSEIGSTLRYVLKELDSLTEHVKEEKELKVQVLEDLKHLESVVASKIKEIER 120

Query: 121 DLEKL 125
            LE++
Sbjct: 121 KLEQM 125


>UniRef50_Q1ZGX9 Putative uncharacterized protein n=1 Tax=Psychromonas sp. CNPT3
           RepID=Q1ZGX9_9GAMM
          Length = 127

 Score =  116 bits (290), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 56/127 (44%), Positives = 90/127 (70%), Gaps = 2/127 (1%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKG--EFFAKSVKFKYPRQRKTVV 58
           M+ +L ++ ITN  EI RYSLR E   DILKIYF+K++G  + F++SVKFK+PRQ K + 
Sbjct: 1   MFPHLATISITNTHEIKRYSLRHEGVYDILKIYFKKERGSHQIFSRSVKFKFPRQNKKIN 60

Query: 59  ADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEI 118
           +D     ++ + EI+ +LR II+ELD +  + +++ D+K ++L+D++HLE+V   KI+EI
Sbjct: 61  SDTQPNSFENISEINASLRNIIEELDTLTLQVKTKTDIKSELLEDIKHLETVFNAKINEI 120

Query: 119 EADLEKL 125
           E  ++ L
Sbjct: 121 EEKIKNL 127


>UniRef50_A6VT88 Putative structural protein n=2 Tax=Marinomonas RepID=A6VT88_MARMS
          Length = 126

 Score =  105 bits (261), Expect = 6e-22,   Method: Compositional matrix adjust.
 Identities = 52/125 (41%), Positives = 84/125 (67%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MY  L+S+GIT+ ++I++++LR E  +D+LKIY++++KG    KS KFK+ R  KTV+AD
Sbjct: 1   MYPTLQSMGITSIQDIEKFTLRYEGGHDVLKIYYRREKGSLLPKSKKFKFGRSTKTVLAD 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
           G  Q Y++VQE S  +   ++EL+QI  R       K  ++ +L HLE VV +KI+EI+ 
Sbjct: 61  GGQQTYRQVQEPSLIVLRAMEELEQIVGRQHETKATKEDLIKELEHLEKVVNSKINEIKE 120

Query: 121 DLEKL 125
            ++ +
Sbjct: 121 KIDSM 125


>UniRef50_A4BBX7 Putative uncharacterized protein n=1 Tax=Reinekea blandensis
          MED297 RepID=A4BBX7_9GAMM
          Length = 89

 Score = 81.6 bits (200), Expect = 7e-15,   Method: Compositional matrix adjust.
 Identities = 44/88 (50%), Positives = 57/88 (64%), Gaps = 2/88 (2%)

Query: 1  MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
          MYDNL +LGI + ++I+ YSL  E + DILKI F+  +G  F +  +F YPRQ K V  D
Sbjct: 1  MYDNLLALGIDDLDQINGYSLSTEKDRDILKIRFRPGRGHMFKRYERFIYPRQHKIVTVD 60

Query: 61 GVGQGYK-EVQEISPNLRYIIDELDQIC 87
            G G K  + EIS  LRY+I+ELDQI 
Sbjct: 61 S-GTGSKVHMTEISSTLRYVIEELDQIA 87


>UniRef50_Q2BP51 Putative uncharacterized protein n=1 Tax=Neptuniibacter
           caesariensis RepID=Q2BP51_9GAMM
          Length = 125

 Score = 62.0 bits (149), Expect = 6e-09,   Method: Compositional matrix adjust.
 Identities = 42/124 (33%), Positives = 72/124 (58%), Gaps = 3/124 (2%)

Query: 2   YDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVADG 61
           ++ LK +G  +P  I++++ R E + DILKIY +K +G++ +KS KFK+ R  K+V    
Sbjct: 5   FEVLKEMGFDDPMAIEKFTTRTEGDMDILKIYLRKQQGDWISKSKKFKFKRSDKSV---E 61

Query: 62  VGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEAD 121
           +G  Y      S      + EL+++ + +      K+ +LD++ HLE V+T KI +I   
Sbjct: 62  MGTAYGAASAPSSYFLKALSELEKLIKVEHDADSRKQVLLDEIDHLEKVMTRKIEDIRRQ 121

Query: 122 LEKL 125
           LE+L
Sbjct: 122 LEEL 125


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_B5F8S5 UPF0325 protein yaeH n=172 Tax=Gammaproteobacter...   181   8e-45
UniRef50_Q2NRK3 UPF0325 protein SG1947 n=55 Tax=Gammaproteobacte...   178   5e-44
UniRef50_A1SSV2 UPF0325 protein Ping_0715 n=1 Tax=Psychromonas i...   153   2e-36
UniRef50_A6VT88 Putative structural protein n=2 Tax=Marinomonas ...   151   6e-36
UniRef50_Q1ZGX9 Putative uncharacterized protein n=1 Tax=Psychro...   148   5e-35
UniRef50_Q2BP51 Putative uncharacterized protein n=1 Tax=Neptuni...   123   2e-27
UniRef50_A4BBX7 Putative uncharacterized protein n=1 Tax=Reineke...   116   3e-25

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_B5F8S5 UPF0325 protein yaeH n=172 Tax=Gammaproteobacteria RepID=YAEH_SALA4
          Length = 128

 Score =  181 bits (458), Expect = 8e-45,   Method: Composition-based stats.
 Identities = 122/128 (95%), Positives = 127/128 (99%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKD+GEFFAKSVKFKYPRQRKTVVAD
Sbjct: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDRGEFFAKSVKFKYPRQRKTVVAD 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
           G+GQGYKEVQEISPNLRY+IDELDQICQRDRSE+DLKRKILDDLRHLESVV NKISEIEA
Sbjct: 61  GIGQGYKEVQEISPNLRYVIDELDQICQRDRSELDLKRKILDDLRHLESVVANKISEIEA 120

Query: 121 DLEKLTRK 128
           DL+KLTRK
Sbjct: 121 DLDKLTRK 128


>UniRef50_Q2NRK3 UPF0325 protein SG1947 n=55 Tax=Gammaproteobacteria
           RepID=Y1947_SODGM
          Length = 129

 Score =  178 bits (451), Expect = 5e-44,   Method: Composition-based stats.
 Identities = 102/128 (79%), Positives = 118/128 (92%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MYDNLKSLG+ +P++IDRYSLRQEANNDILKIYF++DKGE FAKSVKFKYPRQRKTVV+ 
Sbjct: 1   MYDNLKSLGVNHPDDIDRYSLRQEANNDILKIYFRRDKGELFAKSVKFKYPRQRKTVVSY 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
              +GY+E+  ISPNLRY+IDELDQIC RD++EVDLK KILDDLRHLESVV+NKI+EIEA
Sbjct: 61  NAAEGYREINGISPNLRYVIDELDQICLRDQTEVDLKNKILDDLRHLESVVSNKIAEIEA 120

Query: 121 DLEKLTRK 128
           DLEKLTR+
Sbjct: 121 DLEKLTRQ 128


>UniRef50_A1SSV2 UPF0325 protein Ping_0715 n=1 Tax=Psychromonas ingrahamii 37
           RepID=Y715_PSYIN
          Length = 125

 Score =  153 bits (387), Expect = 2e-36,   Method: Composition-based stats.
 Identities = 65/125 (52%), Positives = 91/125 (72%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MY NLKS+G+ N + ID+Y+LRQE + DILKIYF+K   +F AKS+KFK+PRQRK +  D
Sbjct: 1   MYSNLKSIGVVNYDNIDKYTLRQEGDTDILKIYFRKASRQFLAKSLKFKFPRQRKKISQD 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
               G++ V EI   LRY++ ELD + +  + E +LK ++L+DL+HLESVV +KI EIE 
Sbjct: 61  HGSSGFRNVSEIGSTLRYVLKELDSLTEHVKEEKELKVQVLEDLKHLESVVASKIKEIER 120

Query: 121 DLEKL 125
            LE++
Sbjct: 121 KLEQM 125


>UniRef50_A6VT88 Putative structural protein n=2 Tax=Marinomonas RepID=A6VT88_MARMS
          Length = 126

 Score =  151 bits (381), Expect = 6e-36,   Method: Composition-based stats.
 Identities = 52/125 (41%), Positives = 84/125 (67%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
           MY  L+S+GIT+ ++I++++LR E  +D+LKIY++++KG    KS KFK+ R  KTV+AD
Sbjct: 1   MYPTLQSMGITSIQDIEKFTLRYEGGHDVLKIYYRREKGSLLPKSKKFKFGRSTKTVLAD 60

Query: 61  GVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEA 120
           G  Q Y++VQE S  +   ++EL+QI  R       K  ++ +L HLE VV +KI+EI+ 
Sbjct: 61  GGQQTYRQVQEPSLIVLRAMEELEQIVGRQHETKATKEDLIKELEHLEKVVNSKINEIKE 120

Query: 121 DLEKL 125
            ++ +
Sbjct: 121 KIDSM 125


>UniRef50_Q1ZGX9 Putative uncharacterized protein n=1 Tax=Psychromonas sp. CNPT3
           RepID=Q1ZGX9_9GAMM
          Length = 127

 Score =  148 bits (374), Expect = 5e-35,   Method: Composition-based stats.
 Identities = 56/127 (44%), Positives = 90/127 (70%), Gaps = 2/127 (1%)

Query: 1   MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKG--EFFAKSVKFKYPRQRKTVV 58
           M+ +L ++ ITN  EI RYSLR E   DILKIYF+K++G  + F++SVKFK+PRQ K + 
Sbjct: 1   MFPHLATISITNTHEIKRYSLRHEGVYDILKIYFKKERGSHQIFSRSVKFKFPRQNKKIN 60

Query: 59  ADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEI 118
           +D     ++ + EI+ +LR II+ELD +  + +++ D+K ++L+D++HLE+V   KI+EI
Sbjct: 61  SDTQPNSFENISEINASLRNIIEELDTLTLQVKTKTDIKSELLEDIKHLETVFNAKINEI 120

Query: 119 EADLEKL 125
           E  ++ L
Sbjct: 121 EEKIKNL 127


>UniRef50_Q2BP51 Putative uncharacterized protein n=1 Tax=Neptuniibacter
           caesariensis RepID=Q2BP51_9GAMM
          Length = 125

 Score =  123 bits (308), Expect = 2e-27,   Method: Composition-based stats.
 Identities = 42/124 (33%), Positives = 72/124 (58%), Gaps = 3/124 (2%)

Query: 2   YDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVADG 61
           ++ LK +G  +P  I++++ R E + DILKIY +K +G++ +KS KFK+ R  K+V    
Sbjct: 5   FEVLKEMGFDDPMAIEKFTTRTEGDMDILKIYLRKQQGDWISKSKKFKFKRSDKSV---E 61

Query: 62  VGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILDDLRHLESVVTNKISEIEAD 121
           +G  Y      S      + EL+++ + +      K+ +LD++ HLE V+T KI +I   
Sbjct: 62  MGTAYGAASAPSSYFLKALSELEKLIKVEHDADSRKQVLLDEIDHLEKVMTRKIEDIRRQ 121

Query: 122 LEKL 125
           LE+L
Sbjct: 122 LEEL 125


>UniRef50_A4BBX7 Putative uncharacterized protein n=1 Tax=Reinekea blandensis
          MED297 RepID=A4BBX7_9GAMM
          Length = 89

 Score =  116 bits (290), Expect = 3e-25,   Method: Composition-based stats.
 Identities = 41/89 (46%), Positives = 54/89 (60%)

Query: 1  MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAKSVKFKYPRQRKTVVAD 60
          MYDNL +LGI + ++I+ YSL  E + DILKI F+  +G  F +  +F YPRQ K V  D
Sbjct: 1  MYDNLLALGIDDLDQINGYSLSTEKDRDILKIRFRPGRGHMFKRYERFIYPRQHKIVTVD 60

Query: 61 GVGQGYKEVQEISPNLRYIIDELDQICQR 89
                  + EIS  LRY+I+ELDQI   
Sbjct: 61 SGTGSKVHMTEISSTLRYVIEELDQIACH 89


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.304    0.136    0.327 

Lambda     K      H
   0.267   0.0418    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 421,684,334
Number of Sequences: 3077464
Number of extensions: 14520200
Number of successful extensions: 72273
Number of sequences better than 1.0e-01: 10
Number of HSP's better than  0.1 without gapping: 14
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 72248
Number of HSP's gapped (non-prelim): 24
length of query: 128
length of database: 1,040,396,356
effective HSP length: 94
effective length of query: 34
effective length of database: 751,114,740
effective search space: 25537901160
effective search space used: 25537901160
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (20.9 bits)
S2: 87 (38.1 bits)
