BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (59 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P64621 Uncharacterized protein yhdU n=88 Tax=Enterobact...   121   8e-27
UniRef50_D2TNB8 Predicted membrane protein yhdu n=1 Tax=Citrobac...    89   3e-17

>UniRef50_P64621 Uncharacterized protein yhdU n=88 Tax=Enterobacteriaceae
          RepID=YHDU_ECO57
          Length = 59

 Score =  121 bits (303), Expect = 8e-27,   Method: Compositional matrix adjust.
 Identities = 59/59 (100%), Positives = 59/59 (100%)

Query: 1  MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELNFQDRM 59
          MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELNFQDRM
Sbjct: 1  MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELNFQDRM 59


>UniRef50_D2TNB8 Predicted membrane protein yhdu n=1 Tax=Citrobacter rodentium
          ICC168 RepID=D2TNB8_CITRO
          Length = 54

 Score = 89.4 bits (220), Expect = 3e-17,   Method: Compositional matrix adjust.
 Identities = 41/54 (75%), Positives = 47/54 (87%)

Query: 1  MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELN 54
          MIRKYWWLVVFAV VFLFD LL QW+E + TE+DKCRNM+SVNPL+LVNC +L 
Sbjct: 1  MIRKYWWLVVFAVTVFLFDALLFQWLETITTESDKCRNMDSVNPLRLVNCADLK 54


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P64621 Uncharacterized protein yhdU n=88 Tax=Enterobact...    89   4e-17
UniRef50_D2TNB8 Predicted membrane protein yhdu n=1 Tax=Citrobac...    82   4e-15

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P64621 Uncharacterized protein yhdU n=88 Tax=Enterobacteriaceae
          RepID=YHDU_ECO57
          Length = 59

 Score = 89.2 bits (220), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 59/59 (100%), Positives = 59/59 (100%)

Query: 1  MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELNFQDRM 59
          MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELNFQDRM
Sbjct: 1  MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELNFQDRM 59


>UniRef50_D2TNB8 Predicted membrane protein yhdu n=1 Tax=Citrobacter rodentium
          ICC168 RepID=D2TNB8_CITRO
          Length = 54

 Score = 82.3 bits (202), Expect = 4e-15,   Method: Composition-based stats.
 Identities = 41/54 (75%), Positives = 47/54 (87%)

Query: 1  MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNPLKLVNCDELN 54
          MIRKYWWLVVFAV VFLFD LL QW+E + TE+DKCRNM+SVNPL+LVNC +L 
Sbjct: 1  MIRKYWWLVVFAVTVFLFDALLFQWLETITTESDKCRNMDSVNPLRLVNCADLK 54


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.332    0.149    0.508 

Lambda     K      H
   0.267   0.0444    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 271,594,462
Number of Sequences: 3077464
Number of extensions: 8448784
Number of successful extensions: 40192
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 40188
Number of HSP's gapped (non-prelim): 4
length of query: 59
length of database: 1,040,396,356
effective HSP length: 32
effective length of query: 27
effective length of database: 941,917,508
effective search space: 25431772716
effective search space used: 25431772716
T: 11
A: 40
X1: 16 ( 7.7 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.4 bits)
S2: 87 (38.0 bits)