Processing 1 helix: Transmembrane helix 1 from 112 (in) to 127 (out) : 91.933 Score = 0.091933 Processing 1 helix: Transmembrane helix 1 from 112 (out) to 127 (in) : 92.367 Score = 0.092367 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 23 (out) to 38 (in) : -32.33 Transmembrane helix 3 from 42 (in) to 57 (out) : -46.238 Transmembrane helix 4 from 61 (out) to 76 (in) : -311.33 Transmembrane helix 5 from 80 (in) to 95 (out) : -723.92 Transmembrane helix 6 from 109 (out) to 124 (in) : 42.45 Transmembrane helix 7 from 128 (in) to 143 (out) : -231.209 Transmembrane helix 8 from 147 (out) to 162 (in) : -342.941 Transmembrane helix 9 from 166 (in) to 181 (out) : -357.433 Transmembrane helix 10 from 185 (out) to 200 (in) : -120.795 Transmembrane helix 11 from 204 (in) to 219 (out) : -125.781 Transmembrane helix 12 from 223 (out) to 238 (in) : -31.487 Transmembrane helix 13 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -108.85 Transmembrane helix 2 from 23 (in) to 38 (out) : -40.57 Transmembrane helix 3 from 42 (out) to 57 (in) : -57.362 Transmembrane helix 4 from 61 (in) to 76 (out) : -310.07 Transmembrane helix 5 from 109 (out) to 124 (in) : 42.45 Transmembrane helix 6 from 128 (in) to 143 (out) : -231.209 Transmembrane helix 7 from 147 (out) to 162 (in) : -342.941 Transmembrane helix 8 from 166 (in) to 181 (out) : -357.433 Transmembrane helix 9 from 185 (out) to 200 (in) : -120.795 Transmembrane helix 10 from 204 (in) to 219 (out) : -125.781 Transmembrane helix 11 from 223 (out) to 238 (in) : -31.487 Transmembrane helix 12 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 23 (out) to 38 (in) : -32.33 Transmembrane helix 3 from 42 (in) to 57 (out) : -46.238 Transmembrane helix 4 from 61 (out) to 76 (in) : -311.33 Transmembrane helix 5 from 111 (in) to 126 (out) : 78.288 Transmembrane helix 6 from 130 (out) to 145 (in) : -221.208 Transmembrane helix 7 from 156 (in) to 171 (out) : -272.284 Transmembrane helix 8 from 185 (out) to 200 (in) : -120.795 Transmembrane helix 9 from 204 (in) to 219 (out) : -125.781 Transmembrane helix 10 from 223 (out) to 238 (in) : -31.487 Transmembrane helix 11 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -108.85 Transmembrane helix 2 from 24 (in) to 39 (out) : -33.567 Transmembrane helix 3 from 43 (out) to 58 (in) : -61.984 Transmembrane helix 4 from 111 (in) to 126 (out) : 78.288 Transmembrane helix 5 from 130 (out) to 145 (in) : -221.208 Transmembrane helix 6 from 156 (in) to 171 (out) : -272.284 Transmembrane helix 7 from 185 (out) to 200 (in) : -120.795 Transmembrane helix 8 from 204 (in) to 219 (out) : -125.781 Transmembrane helix 9 from 223 (out) to 238 (in) : -31.487 Transmembrane helix 10 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 3 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 4 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 5 from 156 (in) to 171 (out) : -272.284 Transmembrane helix 6 from 185 (out) to 200 (in) : -120.795 Transmembrane helix 7 from 204 (in) to 219 (out) : -125.781 Transmembrane helix 8 from 223 (out) to 238 (in) : -31.487 Transmembrane helix 9 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -108.85 Transmembrane helix 2 from 24 (in) to 39 (out) : -33.567 Transmembrane helix 3 from 43 (out) to 58 (in) : -61.984 Transmembrane helix 4 from 111 (in) to 126 (out) : 78.288 Transmembrane helix 5 from 130 (out) to 145 (in) : -221.208 Transmembrane helix 6 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 7 from 218 (out) to 233 (in) : -8.494 Transmembrane helix 8 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 3 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 4 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 6 from 218 (out) to 233 (in) : -8.494 Transmembrane helix 7 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 2 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 3 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 5 from 218 (out) to 233 (in) : -8.494 Transmembrane helix 6 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -20.789 Transmembrane helix 2 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 3 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 4 from 218 (out) to 233 (in) : -8.494 Transmembrane helix 5 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 31 (out) to 51 (in) : -32.993 Transmembrane helix 2 from 112 (in) to 127 (out) : 91.933 Transmembrane helix 3 from 219 (out) to 234 (in) : -7.723 Transmembrane helix 4 from 242 (in) to 257 (out) : -54.151 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -20.789 Transmembrane helix 2 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 3 from 219 (in) to 234 (out) : -1.677 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 31 (out) to 51 (in) : -32.993 Transmembrane helix 2 from 112 (in) to 127 (out) : 91.933 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -108.85 Transmembrane helix 2 from 23 (in) to 38 (out) : -40.57 Transmembrane helix 3 from 42 (out) to 57 (in) : -57.362 Transmembrane helix 4 from 61 (in) to 76 (out) : -310.07 Transmembrane helix 5 from 80 (out) to 95 (in) : -721.88 Transmembrane helix 6 from 109 (in) to 124 (out) : 25.35 Transmembrane helix 7 from 128 (out) to 143 (in) : -207.891 Transmembrane helix 8 from 147 (in) to 162 (out) : -345.759 Transmembrane helix 9 from 166 (out) to 181 (in) : -368.567 Transmembrane helix 10 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 11 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 12 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 13 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 23 (out) to 38 (in) : -32.33 Transmembrane helix 3 from 42 (in) to 57 (out) : -46.238 Transmembrane helix 4 from 61 (out) to 76 (in) : -311.33 Transmembrane helix 5 from 109 (in) to 124 (out) : 25.35 Transmembrane helix 6 from 128 (out) to 143 (in) : -207.891 Transmembrane helix 7 from 147 (in) to 162 (out) : -345.759 Transmembrane helix 8 from 166 (out) to 181 (in) : -368.567 Transmembrane helix 9 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 10 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 11 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 12 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -108.85 Transmembrane helix 2 from 23 (in) to 38 (out) : -40.57 Transmembrane helix 3 from 42 (out) to 57 (in) : -57.362 Transmembrane helix 4 from 61 (in) to 76 (out) : -310.07 Transmembrane helix 5 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 6 from 131 (in) to 146 (out) : -247.418 Transmembrane helix 7 from 153 (out) to 168 (in) : -253.914 Transmembrane helix 8 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 9 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 10 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 11 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 23 (out) to 38 (in) : -32.33 Transmembrane helix 3 from 42 (in) to 57 (out) : -46.238 Transmembrane helix 4 from 61 (out) to 76 (in) : -311.33 Transmembrane helix 5 from 111 (in) to 126 (out) : 78.288 Transmembrane helix 6 from 130 (out) to 145 (in) : -221.208 Transmembrane helix 7 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 8 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 9 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 10 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -108.85 Transmembrane helix 2 from 24 (in) to 39 (out) : -33.567 Transmembrane helix 3 from 43 (out) to 58 (in) : -61.984 Transmembrane helix 4 from 111 (in) to 126 (out) : 78.288 Transmembrane helix 5 from 130 (out) to 145 (in) : -221.208 Transmembrane helix 6 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 7 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 8 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 9 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 3 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 4 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 5 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 6 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 7 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 8 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 2 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 3 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 4 from 185 (in) to 200 (out) : -110.505 Transmembrane helix 5 from 204 (out) to 219 (in) : -115.319 Transmembrane helix 6 from 223 (in) to 238 (out) : -26.513 Transmembrane helix 7 from 242 (out) to 257 (in) : -61.849 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -109.85 Transmembrane helix 2 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 3 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 4 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 5 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 6 from 218 (out) to 233 (in) : -8.494 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 24 (out) to 39 (in) : -26.233 Transmembrane helix 2 from 43 (in) to 58 (out) : -51.716 Transmembrane helix 3 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 4 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 5 from 218 (out) to 233 (in) : -8.494 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 30 (in) to 45 (out) : -20.789 Transmembrane helix 2 from 112 (out) to 127 (in) : 92.367 Transmembrane helix 3 from 187 (in) to 202 (out) : -103.408 Transmembrane helix 4 from 218 (out) to 233 (in) : -8.494 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 31 (out) to 51 (in) : -32.993 Transmembrane helix 2 from 112 (in) to 127 (out) : 91.933 Transmembrane helix 3 from 219 (out) to 234 (in) : -7.723 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 112 (in) to 127 (out) : 91.933 Transmembrane helix 2 from 219 (out) to 234 (in) : -7.723 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.091933 1 helix (-) : Score = 0.092367 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 112-127 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.092367