BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (66 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobac...    84   1e-15

>UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobacteriaceae
          RepID=YEET_ECO57
          Length = 73

 Score = 84.3 bits (207), Expect = 1e-15,   Method: Compositional matrix adjust.
 Identities = 37/58 (63%), Positives = 48/58 (82%)

Query: 1  MRIISKRRAMTIYRQHPESRIFRYCTGKYQWHGSVCHYTGRDVPDITGVLAVYAERRR 58
          M+II++  AM I++QHP SR+F +CTGKY+WHGS   YTGR+V DI GVLAV+AERR+
Sbjct: 1  MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRK 58


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobac...   108   5e-23

Sequences not found previously or not previously below threshold:

UniRef50_B8FNH6 Putative uncharacterized protein n=1 Tax=Desulfa...    41   0.013


CONVERGED!
>UniRef50_P64523 Uncharacterized protein yeeT n=144 Tax=Enterobacteriaceae
          RepID=YEET_ECO57
          Length = 73

 Score =  108 bits (271), Expect = 5e-23,   Method: Composition-based stats.
 Identities = 37/60 (61%), Positives = 49/60 (81%)

Query: 1  MRIISKRRAMTIYRQHPESRIFRYCTGKYQWHGSVCHYTGRDVPDITGVLAVYAERRRTA 60
          M+II++  AM I++QHP SR+F +CTGKY+WHGS   YTGR+V DI GVLAV+AERR+ +
Sbjct: 1  MKIITRGEAMRIHQQHPTSRLFPFCTGKYRWHGSAEAYTGREVQDIPGVLAVFAERRKDS 60


>UniRef50_B8FNH6 Putative uncharacterized protein n=1 Tax=Desulfatibacillum
          alkenivorans AK-01 RepID=B8FNH6_DESAA
          Length = 81

 Score = 40.7 bits (94), Expect = 0.013,   Method: Composition-based stats.
 Identities = 22/67 (32%), Positives = 36/67 (53%), Gaps = 7/67 (10%)

Query: 1  MRIISKRRAMTIYRQ------HPESRIFRYCTGKYQWHGSVCHYTGRDVPDI-TGVLAVY 53
          M+I++K+ A+ +  +      +      +Y +GKY+W G    YTG D+  +   +LAVY
Sbjct: 1  MKIVTKKDAVELASKTILDGKNRAKTPTKYDSGKYKWTGKASDYTGVDIDFVWPELLAVY 60

Query: 54 AERRRTA 60
           ERR  A
Sbjct: 61 VERRSDA 67


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.317    0.141    0.425 

Lambda     K      H
   0.267   0.0412    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 253,207,888
Number of Sequences: 3077464
Number of extensions: 7859501
Number of successful extensions: 22126
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 22123
Number of HSP's gapped (non-prelim): 3
length of query: 66
length of database: 1,040,396,356
effective HSP length: 38
effective length of query: 28
effective length of database: 923,452,724
effective search space: 25856676272
effective search space used: 25856676272
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.1 bits)
S2: 87 (38.1 bits)