Processing 1 helix: Transmembrane helix 1 from 41 (in) to 56 (out) : -26.094 Score = -0.001482 Processing 1 helix: Transmembrane helix 1 from 41 (out) to 56 (in) : -18.306 Score = -0.042918 Processing 20 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -1316.09 Transmembrane helix 2 from 23 (out) to 38 (in) : -698.997 Transmembrane helix 3 from 42 (in) to 57 (out) : -35.531 Transmembrane helix 4 from 61 (out) to 76 (in) : -779.99 Transmembrane helix 5 from 80 (in) to 95 (out) : -1148.77 Transmembrane helix 6 from 99 (out) to 114 (in) : -1366.92 Transmembrane helix 7 from 118 (in) to 133 (out) : -1046.73 Transmembrane helix 8 from 137 (out) to 152 (in) : -711.545 Transmembrane helix 9 from 156 (in) to 171 (out) : -1225.22 Transmembrane helix 10 from 175 (out) to 190 (in) : -1170.52 Transmembrane helix 11 from 194 (in) to 209 (out) : -857.571 Transmembrane helix 12 from 213 (out) to 228 (in) : -986.27 Transmembrane helix 13 from 232 (in) to 247 (out) : -1048.37 Transmembrane helix 14 from 251 (out) to 266 (in) : -1257.78 Transmembrane helix 15 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 16 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 17 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 18 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 19 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 20 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -880.961 Transmembrane helix 2 from 31 (in) to 46 (out) : -550.03 Transmembrane helix 3 from 50 (out) to 65 (in) : -245.27 Transmembrane helix 4 from 69 (in) to 84 (out) : -984.511 Transmembrane helix 5 from 88 (out) to 103 (in) : -1105.05 Transmembrane helix 6 from 113 (in) to 128 (out) : -1152.5 Transmembrane helix 7 from 132 (out) to 147 (in) : -725.245 Transmembrane helix 8 from 151 (in) to 166 (out) : -1152.21 Transmembrane helix 9 from 170 (out) to 185 (in) : -1164.66 Transmembrane helix 10 from 189 (in) to 204 (out) : -1015.2 Transmembrane helix 11 from 208 (out) to 223 (in) : -896.532 Transmembrane helix 12 from 227 (in) to 242 (out) : -988.486 Transmembrane helix 13 from 246 (out) to 261 (in) : -1136.87 Transmembrane helix 14 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 15 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 16 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 17 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 18 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 19 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -882.539 Transmembrane helix 2 from 31 (out) to 46 (in) : -562.57 Transmembrane helix 3 from 50 (in) to 65 (out) : -250.53 Transmembrane helix 4 from 69 (out) to 84 (in) : -988.989 Transmembrane helix 5 from 88 (in) to 103 (out) : -1112.95 Transmembrane helix 6 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 7 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 8 from 158 (out) to 173 (in) : -1222.61 Transmembrane helix 9 from 178 (in) to 193 (out) : -1127.17 Transmembrane helix 10 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 11 from 221 (in) to 236 (out) : -931.522 Transmembrane helix 12 from 240 (out) to 255 (in) : -1085.75 Transmembrane helix 13 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 14 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 15 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 16 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 17 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 18 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -880.961 Transmembrane helix 2 from 31 (in) to 46 (out) : -550.03 Transmembrane helix 3 from 50 (out) to 65 (in) : -245.27 Transmembrane helix 4 from 69 (in) to 84 (out) : -984.511 Transmembrane helix 5 from 88 (out) to 103 (in) : -1105.05 Transmembrane helix 6 from 120 (in) to 135 (out) : -981.398 Transmembrane helix 7 from 139 (out) to 154 (in) : -749.926 Transmembrane helix 8 from 178 (in) to 193 (out) : -1127.17 Transmembrane helix 9 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 10 from 221 (in) to 236 (out) : -931.522 Transmembrane helix 11 from 240 (out) to 255 (in) : -1085.75 Transmembrane helix 12 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 13 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 14 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 15 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 16 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 17 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 88 (out) to 103 (in) : -1105.05 Transmembrane helix 5 from 120 (in) to 135 (out) : -981.398 Transmembrane helix 6 from 139 (out) to 154 (in) : -749.926 Transmembrane helix 7 from 178 (in) to 193 (out) : -1127.17 Transmembrane helix 8 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 9 from 221 (in) to 236 (out) : -931.522 Transmembrane helix 10 from 240 (out) to 255 (in) : -1085.75 Transmembrane helix 11 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 12 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 13 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 14 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 15 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 16 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 88 (in) to 103 (out) : -1112.95 Transmembrane helix 5 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 6 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 7 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 8 from 221 (in) to 236 (out) : -931.522 Transmembrane helix 9 from 240 (out) to 255 (in) : -1085.75 Transmembrane helix 10 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 11 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 12 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 13 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 14 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 15 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 5 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 6 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 7 from 221 (in) to 236 (out) : -931.522 Transmembrane helix 8 from 240 (out) to 255 (in) : -1085.75 Transmembrane helix 9 from 270 (in) to 285 (out) : -395.863 Transmembrane helix 10 from 289 (out) to 304 (in) : -210.581 Transmembrane helix 11 from 308 (in) to 323 (out) : -622.023 Transmembrane helix 12 from 327 (out) to 342 (in) : -683.651 Transmembrane helix 13 from 346 (in) to 361 (out) : -764.636 Transmembrane helix 14 from 365 (out) to 380 (in) : -1055.51 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 88 (in) to 103 (out) : -1112.95 Transmembrane helix 5 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 6 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 7 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 8 from 222 (in) to 237 (out) : -927.805 Transmembrane helix 9 from 271 (out) to 286 (in) : -334.707 Transmembrane helix 10 from 290 (in) to 305 (out) : -221.908 Transmembrane helix 11 from 309 (out) to 324 (in) : -645.928 Transmembrane helix 12 from 328 (in) to 343 (out) : -663.494 Transmembrane helix 13 from 347 (out) to 362 (in) : -788.825 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 5 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 6 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 7 from 222 (in) to 237 (out) : -927.805 Transmembrane helix 8 from 271 (out) to 286 (in) : -334.707 Transmembrane helix 9 from 290 (in) to 305 (out) : -221.908 Transmembrane helix 10 from 309 (out) to 324 (in) : -645.928 Transmembrane helix 11 from 328 (in) to 343 (out) : -663.494 Transmembrane helix 12 from 347 (out) to 362 (in) : -788.825 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 5 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 6 from 222 (in) to 237 (out) : -927.805 Transmembrane helix 7 from 271 (out) to 286 (in) : -334.707 Transmembrane helix 8 from 290 (in) to 305 (out) : -221.908 Transmembrane helix 9 from 309 (out) to 324 (in) : -645.928 Transmembrane helix 10 from 328 (in) to 343 (out) : -663.494 Transmembrane helix 11 from 347 (out) to 362 (in) : -788.825 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 137 (out) to 152 (in) : -711.545 Transmembrane helix 5 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 6 from 271 (out) to 286 (in) : -334.707 Transmembrane helix 7 from 290 (in) to 305 (out) : -221.908 Transmembrane helix 8 from 309 (out) to 324 (in) : -645.928 Transmembrane helix 9 from 328 (in) to 343 (out) : -663.494 Transmembrane helix 10 from 347 (out) to 362 (in) : -788.825 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 5 from 199 (out) to 214 (in) : -717.428 Transmembrane helix 6 from 276 (in) to 291 (out) : -151.945 Transmembrane helix 7 from 295 (out) to 310 (in) : -293.046 Transmembrane helix 8 from 314 (in) to 329 (out) : -720.728 Transmembrane helix 9 from 333 (out) to 348 (in) : -668.847 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 4 from 199 (out) to 214 (in) : -717.428 Transmembrane helix 5 from 276 (in) to 291 (out) : -151.945 Transmembrane helix 6 from 295 (out) to 310 (in) : -293.046 Transmembrane helix 7 from 314 (in) to 329 (out) : -720.728 Transmembrane helix 8 from 333 (out) to 348 (in) : -668.847 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 3 from 137 (out) to 152 (in) : -711.545 Transmembrane helix 4 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 5 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 6 from 296 (in) to 311 (out) : -305.468 Transmembrane helix 7 from 331 (out) to 346 (in) : -655.592 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 4 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 5 from 296 (in) to 311 (out) : -305.468 Transmembrane helix 6 from 331 (out) to 346 (in) : -655.592 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 3 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 4 from 296 (in) to 311 (out) : -305.468 Transmembrane helix 5 from 331 (out) to 346 (in) : -655.592 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 2 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 3 from 296 (in) to 311 (out) : -305.468 Transmembrane helix 4 from 331 (out) to 346 (in) : -655.592 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 2 from 277 (in) to 292 (out) : -137.295 Transmembrane helix 3 from 296 (out) to 311 (in) : -304.932 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 2 from 278 (out) to 293 (in) : -134.8 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -1308.01 Transmembrane helix 2 from 23 (in) to 38 (out) : -696.603 Transmembrane helix 3 from 42 (out) to 57 (in) : -21.469 Transmembrane helix 4 from 61 (in) to 76 (out) : -771.91 Transmembrane helix 5 from 80 (out) to 95 (in) : -1158.83 Transmembrane helix 6 from 99 (in) to 114 (out) : -1368.58 Transmembrane helix 7 from 118 (out) to 133 (in) : -1045.07 Transmembrane helix 8 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 9 from 156 (out) to 171 (in) : -1230.58 Transmembrane helix 10 from 175 (in) to 190 (out) : -1172.38 Transmembrane helix 11 from 194 (out) to 209 (in) : -858.529 Transmembrane helix 12 from 213 (in) to 228 (out) : -987.63 Transmembrane helix 13 from 232 (out) to 247 (in) : -1039.03 Transmembrane helix 14 from 251 (in) to 266 (out) : -1264.61 Transmembrane helix 15 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 16 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 17 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 18 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 19 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 20 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -882.539 Transmembrane helix 2 from 31 (out) to 46 (in) : -562.57 Transmembrane helix 3 from 50 (in) to 65 (out) : -250.53 Transmembrane helix 4 from 69 (out) to 84 (in) : -988.989 Transmembrane helix 5 from 88 (in) to 103 (out) : -1112.95 Transmembrane helix 6 from 107 (out) to 122 (in) : -1345.25 Transmembrane helix 7 from 126 (in) to 141 (out) : -815.836 Transmembrane helix 8 from 145 (out) to 160 (in) : -969.093 Transmembrane helix 9 from 164 (in) to 179 (out) : -1206.73 Transmembrane helix 10 from 183 (out) to 198 (in) : -1172.68 Transmembrane helix 11 from 202 (in) to 217 (out) : -710.708 Transmembrane helix 12 from 221 (out) to 236 (in) : -933.978 Transmembrane helix 13 from 240 (in) to 255 (out) : -1082.35 Transmembrane helix 14 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 15 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 16 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 17 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 18 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 19 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -880.961 Transmembrane helix 2 from 31 (in) to 46 (out) : -550.03 Transmembrane helix 3 from 50 (out) to 65 (in) : -245.27 Transmembrane helix 4 from 69 (in) to 84 (out) : -984.511 Transmembrane helix 5 from 88 (out) to 103 (in) : -1105.05 Transmembrane helix 6 from 120 (in) to 135 (out) : -981.398 Transmembrane helix 7 from 139 (out) to 154 (in) : -749.926 Transmembrane helix 8 from 158 (in) to 173 (out) : -1216.09 Transmembrane helix 9 from 179 (out) to 194 (in) : -1122.57 Transmembrane helix 10 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 11 from 221 (out) to 236 (in) : -933.978 Transmembrane helix 12 from 240 (in) to 255 (out) : -1082.35 Transmembrane helix 13 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 14 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 15 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 16 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 17 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 18 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 88 (out) to 103 (in) : -1105.05 Transmembrane helix 5 from 120 (in) to 135 (out) : -981.398 Transmembrane helix 6 from 139 (out) to 154 (in) : -749.926 Transmembrane helix 7 from 158 (in) to 173 (out) : -1216.09 Transmembrane helix 8 from 179 (out) to 194 (in) : -1122.57 Transmembrane helix 9 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 10 from 221 (out) to 236 (in) : -933.978 Transmembrane helix 11 from 240 (in) to 255 (out) : -1082.35 Transmembrane helix 12 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 13 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 14 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 15 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 16 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 17 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 88 (in) to 103 (out) : -1112.95 Transmembrane helix 5 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 6 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 7 from 179 (out) to 194 (in) : -1122.57 Transmembrane helix 8 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 9 from 221 (out) to 236 (in) : -933.978 Transmembrane helix 10 from 240 (in) to 255 (out) : -1082.35 Transmembrane helix 11 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 12 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 13 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 14 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 15 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 16 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 5 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 6 from 179 (out) to 194 (in) : -1122.57 Transmembrane helix 7 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 8 from 221 (out) to 236 (in) : -933.978 Transmembrane helix 9 from 240 (in) to 255 (out) : -1082.35 Transmembrane helix 10 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 11 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 12 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 13 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 14 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 15 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 88 (in) to 103 (out) : -1112.95 Transmembrane helix 5 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 6 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 7 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 8 from 222 (in) to 237 (out) : -927.805 Transmembrane helix 9 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 10 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 11 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 12 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 13 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 14 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 120 (out) to 135 (in) : -979.302 Transmembrane helix 5 from 139 (in) to 154 (out) : -747.874 Transmembrane helix 6 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 7 from 222 (in) to 237 (out) : -927.805 Transmembrane helix 8 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 9 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 10 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 11 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 12 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 13 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 5 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 6 from 222 (in) to 237 (out) : -927.805 Transmembrane helix 7 from 270 (out) to 285 (in) : -392.037 Transmembrane helix 8 from 289 (in) to 304 (out) : -200.019 Transmembrane helix 9 from 308 (out) to 323 (in) : -630.677 Transmembrane helix 10 from 327 (in) to 342 (out) : -684.249 Transmembrane helix 11 from 346 (out) to 361 (in) : -761.364 Transmembrane helix 12 from 365 (in) to 380 (out) : -1044.09 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 137 (out) to 152 (in) : -711.545 Transmembrane helix 5 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 6 from 223 (out) to 238 (in) : -932.595 Transmembrane helix 7 from 271 (in) to 286 (out) : -336.993 Transmembrane helix 8 from 290 (out) to 305 (in) : -232.692 Transmembrane helix 9 from 309 (in) to 324 (out) : -635.972 Transmembrane helix 10 from 328 (out) to 343 (in) : -665.206 Transmembrane helix 11 from 347 (in) to 362 (out) : -791.175 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 40 (in) to 55 (out) : -37.832 Transmembrane helix 3 from 59 (out) to 74 (in) : -759.264 Transmembrane helix 4 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 5 from 201 (out) to 216 (in) : -699.009 Transmembrane helix 6 from 271 (in) to 286 (out) : -336.993 Transmembrane helix 7 from 290 (out) to 305 (in) : -232.692 Transmembrane helix 8 from 309 (in) to 324 (out) : -635.972 Transmembrane helix 9 from 328 (out) to 343 (in) : -665.206 Transmembrane helix 10 from 347 (in) to 362 (out) : -791.175 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 62 (in) to 77 (out) : -761.338 Transmembrane helix 4 from 137 (out) to 152 (in) : -711.545 Transmembrane helix 5 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 6 from 275 (out) to 290 (in) : -179.655 Transmembrane helix 7 from 294 (in) to 309 (out) : -283.62 Transmembrane helix 8 from 313 (out) to 328 (in) : -712.601 Transmembrane helix 9 from 332 (in) to 347 (out) : -652.387 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 3 from 137 (out) to 152 (in) : -711.545 Transmembrane helix 4 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 5 from 275 (out) to 290 (in) : -179.655 Transmembrane helix 6 from 294 (in) to 309 (out) : -283.62 Transmembrane helix 7 from 313 (out) to 328 (in) : -712.601 Transmembrane helix 8 from 332 (in) to 347 (out) : -652.387 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 137 (in) to 152 (out) : -711.755 Transmembrane helix 4 from 200 (out) to 215 (in) : -700.635 Transmembrane helix 5 from 277 (in) to 292 (out) : -137.295 Transmembrane helix 6 from 296 (out) to 311 (in) : -304.932 Transmembrane helix 7 from 332 (in) to 347 (out) : -652.387 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 3 from 200 (out) to 215 (in) : -700.635 Transmembrane helix 4 from 277 (in) to 292 (out) : -137.295 Transmembrane helix 5 from 296 (out) to 311 (in) : -304.932 Transmembrane helix 6 from 332 (in) to 347 (out) : -652.387 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 18 (in) to 33 (out) : -662.697 Transmembrane helix 2 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 3 from 201 (in) to 216 (out) : -695.791 Transmembrane helix 4 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 5 from 296 (in) to 311 (out) : -305.468 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 18 (out) to 33 (in) : -669.503 Transmembrane helix 2 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 3 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 4 from 296 (in) to 311 (out) : -305.468 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -26.094 Transmembrane helix 2 from 277 (out) to 292 (in) : -138.705 Transmembrane helix 3 from 296 (in) to 311 (out) : -305.468 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -18.306 Transmembrane helix 2 from 278 (in) to 293 (out) : -132.8 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.00148201 1 helix (-) : Score = -0.042918 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 41-56 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: -0.00148201