Secondary structure and disorder prediction

		1	.	.	.	.	.	.	.	.	10	.	.	.	.	.	.	.	.	.	20	.	.	.	.	.	.	.	.	.	30	.	.	.	.	.	.	.	.	.	40	.	.	.	.	.	.	.	.	.	50	.	.	.	.	.	.	.	.	.	60
Sequence		M	S	S	L	S	Q	A	A	S	S	V	E	K	R	T	N	A	R	Y	W	I	V	V	M	L	F	I	V	T	S	F	N	Y	G	D	R	A	T	L	S	I	A	G	S	E	M	A	K	D	I	G	L	D	P	V	G	M	G	Y	V
Secondary structure
SS confidence
Disorder		?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	70	.	.	.	.	.	.	.	.	.	80	.	.	.	.	.	.	.	.	.	90	.	.	.	.	.	.	.	.	.	100	.	.	.	.	.	.	.	.	.	110	.	.	.	.	.	.	.	.	.	120
Sequence		F	S	A	F	S	W	A	Y	V	I	G	Q	I	P	G	G	W	L	L	D	R	F	G	S	K	R	V	Y	F	W	S	I	F	I	W	S	M	F	T	L	L	Q	G	F	V	D	I	F	S	G	F	G	I	I	V	A	L	F	T	L
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	130	.	.	.	.	.	.	.	.	.	140	.	.	.	.	.	.	.	.	.	150	.	.	.	.	.	.	.	.	.	160	.	.	.	.	.	.	.	.	.	170	.	.	.	.	.	.	.	.	.	180
Sequence		R	F	L	V	G	L	A	E	A	P	S	F	P	G	N	S	R	I	V	A	A	W	F	P	A	Q	E	R	G	T	A	V	S	I	F	N	S	A	Q	Y	F	A	T	V	I	F	A	P	I	M	G	W	L	T	H	E	V	G	W	S
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	190	.	.	.	.	.	.	.	.	.	200	.	.	.	.	.	.	.	.	.	210	.	.	.	.	.	.	.	.	.	220	.	.	.	.	.	.	.	.	.	230	.	.	.	.	.	.	.	.	.	240
Sequence		H	V	F	F	F	M	G	G	L	G	I	V	I	S	F	I	W	L	K	V	I	H	E	P	N	Q	H	P	G	V	N	K	K	E	L	E	Y	I	A	A	G	G	A	L	I	N	M	D	Q	Q	N	T	K	V	K	V	P	F	S	V
Secondary structure
SS confidence
Disorder																														?											?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	250	.	.	.	.	.	.	.	.	.	260	.	.	.	.	.	.	.	.	.	270	.	.	.	.	.	.	.	.	.	280	.	.	.	.	.	.	.	.	.	290	.	.	.	.	.	.	.	.	.	300
Sequence		K	W	G	Q	I	K	Q	L	L	G	S	R	M	M	I	G	V	Y	I	G	Q	Y	C	I	N	A	L	T	Y	F	F	I	T	W	F	P	V	Y	L	V	Q	A	R	G	M	S	I	L	K	A	G	F	V	A	S	V	P	A	V	C
Secondary structure
SS confidence
Disorder		?	?																																										?
Disorder confidence
		.	.	.	.	.	.	.	.	.	310	.	.	.	.	.	.	.	.	.	320	.	.	.	.	.	.	.	.	.	330	.	.	.	.	.	.	.	.	.	340	.	.	.	.	.	.	.	.	.	350	.	.	.	.	.	.	.	.	.	360
Sequence		G	F	I	G	G	V	L	G	G	I	I	S	D	W	L	M	R	R	T	G	S	L	N	I	A	R	K	T	P	I	V	M	G	M	L	L	S	M	V	M	V	F	C	N	Y	V	N	V	E	W	M	I	I	G	F	M	A	L	A	F
Secondary structure
SS confidence
Disorder
Disorder confidence
		.	.	.	.	.	.	.	.	.	370	.	.	.	.	.	.	.	.	.	380	.	.	.	.	.	.	.	.	.	390	.	.	.	.	.	.	.	.	.	400	.	.	.	.	.	.	.	.	.	410	.	.	.	.	.	.	.	.	.	420
Sequence		F	G	K	G	I	G	A	L	G	W	A	V	M	A	D	T	A	P	K	E	I	S	G	L	S	G	G	L	F	N	M	F	G	N	I	S	G	I	V	T	P	I	A	I	G	Y	I	V	G	T	T	G	S	F	N	G	A	L	I	Y
Secondary structure
SS confidence
Disorder																		?	?
Disorder confidence
		.	.	.	.	.	.	.	.	.	430	.	.	.	.	.	.	.	.	.	440	.	.	.	.	.	.	.	.	.	450
Sequence		V	G	V	H	A	L	I	A	V	L	S	Y	L	V	L	V	G	D	I	K	R	I	E	L	K	P	V	A	G	Q
Secondary structure
SS confidence
Disorder																			?	?	?	?	?	?	?	?	?	?	?	?	?
Disorder confidence

Confidence Key
High(9)											Low (0)

?	Disordered
	Alpha helix
	Beta strand

Domain analysis

Hover over an aligned region to see model and summary info

Please note, only up to the top 20 hits are modelled to reduce computer load

Rank Aligned region

PDB 1pw4 chain A Region: 2 - 449 Aligned: 424 Modelled: 430 Confidence: 100.0% Identity: 19% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: Glycerol-3-phosphate transporter Phyre2 PDB 3o7p chain A Region: 24 - 437 Aligned: 405 Modelled: 414 Confidence: 100.0% Identity: 10% PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) Phyre2 PDB 2gfp chain A Region: 21 - 427 Aligned: 369 Modelled: 374 Confidence: 100.0% Identity: 17% PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli Phyre2 PDB 1pv7 chain A Region: 18 - 446 Aligned: 404 Modelled: 405 Confidence: 100.0% Identity: 11% Fold: MFS general substrate transporter Superfamily: MFS general substrate transporter Family: LacY-like proton/sugar symporter Phyre2 PDB 2xut chain C Region: 23 - 436 Aligned: 409 Modelled: 414 Confidence: 100.0% Identity: 14% PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. Phyre2 PDB 3b9y chain A Region: 12 - 246 Aligned: 223 Modelled: 235 Confidence: 44.4% Identity: 9% PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein Phyre2 PDB 2g9p chain A Region: 74 - 87 Aligned: 14 Modelled: 14 Confidence: 16.6% Identity: 36% PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom Phyre2 PDB 3pro chain C domain 1 Region: 42 - 83 Aligned: 42 Modelled: 42 Confidence: 6.7% Identity: 14% Fold: Alpha-lytic protease prodomain-like Superfamily: Alpha-lytic protease prodomain Family: Alpha-lytic protease prodomain Phyre2 PDB 2rdd chain B Region: 424 - 445 Aligned: 22 Modelled: 22 Confidence: 6.4% Identity: 5% PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. Phyre2 PDB 3qnq chain D Region: 401 - 450 Aligned: 50 Modelled: 50 Confidence: 6.1% Identity: 20% PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system Phyre2 PDB 3rko chain F Region: 408 - 443 Aligned: 36 Modelled: 36 Confidence: 5.9% Identity: 11% PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution Phyre2 PDB 2xq2 chain A Region: 293 - 450 Aligned: 154 Modelled: 158 Confidence: 5.2% Identity: 6% PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt Phyre2 1 d1pw4a_ 2 c3o7pA_ 3 c2gfpA_ 4 d1pv7a_ 5 c2xutC_ 6 c3b9yA_ 7 c2g9pA_ 8 d3proc1 9 c2rddB_ 10 c3qnqD_ 11 c3rkoF_ 12 c2xq2A_

Detailed template information

# Template Alignment Coverage 3D Model Confidence % i.d. Template Information

1 d1pw4a_ 100.0 19 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:Glycerol-3-phosphate transporter 2 c3o7pA_ 100.0 10 PDB header:transport protein Chain: A: PDB Molecule:l-fucose-proton symporter; PDBTitle: crystal structure of the e.coli fucose:proton symporter, fucp (n162a) 3 c2gfpA_ 100.0 17 PDB header:membrane protein Chain: A: PDB Molecule:multidrug resistance protein d; PDBTitle: structure of the multidrug transporter emrd from2 escherichia coli 4 d1pv7a_ 100.0 11 Fold:MFS general substrate transporter Superfamily:MFS general substrate transporter Family:LacY-like proton/sugar symporter 5 c2xutC_ 100.0 14 PDB header:transport protein Chain: C: PDB Molecule:proton/peptide symporter family protein; PDBTitle: crystal structure of a proton dependent oligopeptide (pot)2 family transporter. 6 c3b9yA_ 44.4 9 PDB header:transport protein Chain: A: PDB Molecule:ammonium transporter family rh-like protein; PDBTitle: crystal structure of the nitrosomonas europaea rh protein 7 c2g9pA_ 16.6 36 PDB header:antimicrobial protein Chain: A: PDB Molecule:antimicrobial peptide latarcin 2a; PDBTitle: nmr structure of a novel antimicrobial peptide, latarcin 2a,2 from spider (lachesana tarabaevi) venom 8 d3proc1 6.7 14 Fold:Alpha-lytic protease prodomain-like Superfamily:Alpha-lytic protease prodomain Family:Alpha-lytic protease prodomain 9 c2rddB_ 6.4 5 PDB header:membrane protein/transport protein Chain: B: PDB Molecule:upf0092 membrane protein yajc; PDBTitle: x-ray crystal structure of acrb in complex with a novel2 transmembrane helix. 10 c3qnqD_ 6.1 20 PDB header:membrane protein, transport protein Chain: D: PDB Molecule:pts system, cellobiose-specific iic component; PDBTitle: crystal structure of the transporter chbc, the iic component from the2 n,n'-diacetylchitobiose-specific phosphotransferase system 11 c3rkoF_ 5.9 11 PDB header:oxidoreductase Chain: F: PDB Molecule:nadh-quinone oxidoreductase subunit j; PDBTitle: crystal structure of the membrane domain of respiratory complex i from2 e. coli at 3.0 angstrom resolution 12 c2xq2A_ 5.2 6 PDB header:transport protein Chain: A: PDB Molecule:sodium/glucose cotransporter; PDBTitle: structure of the k294a mutant of vsglt

Binding site prediction

Due to computational demand, binding site predictions are not run for batch jobs

If you want to predict binding sites, please manually submit your model of choice to 3DLigandSite

Transmembrane helix prediction

Transmembrane helices have been predicted in your sequence to adopt the topology shown below

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Phyre is for academic use only

Please cite: Protein structure prediction on the web: a case study using the Phyre server
Kelley LA and Sternberg MJE. Nature Protocols 4, 363 - 371 (2009) [pdf] [Import into BibTeX]
If you use the binding site predictions from 3DLigandSite, please also cite:
3DLigandSite: predicting ligand-binding sites using similar structures.
Wass MN, Kelley LA and Sternberg MJ Nucleic Acids Research 38, W469-73 (2010) [PubMed]
© Structural Bioinformatics Group Imperial College London Lawrence Kelley, Benjamin Jefferys Disclaimer Terms and Conditions	Component software Template detection: HHpred 1.51 Secondary structure prediction: Psi-pred 2.5 Disorder prediction: Disopred 2.4 Transmembrane prediction: Memsat_SVM Multi-template modelling and ab initio: Poing 1.0