Processing 1 helix: Transmembrane helix 1 from 141 (in) to 165 (out) : 4023.21 Score = 4.023209 Processing 1 helix: Transmembrane helix 1 from 141 (out) to 165 (in) : 3945.79 Score = 3.945791 Processing 15 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -3150.76 Transmembrane helix 2 from 24 (out) to 39 (in) : -240.169 Transmembrane helix 3 from 43 (in) to 58 (out) : 1025.26 Transmembrane helix 4 from 62 (out) to 77 (in) : -583.838 Transmembrane helix 5 from 81 (in) to 96 (out) : -706.731 Transmembrane helix 6 from 100 (out) to 115 (in) : 1210.53 Transmembrane helix 7 from 119 (in) to 134 (out) : -456.585 Transmembrane helix 8 from 138 (out) to 153 (in) : 2441.71 Transmembrane helix 9 from 157 (in) to 172 (out) : 714.975 Transmembrane helix 10 from 176 (out) to 191 (in) : 1548.55 Transmembrane helix 11 from 195 (in) to 210 (out) : 368.276 Transmembrane helix 12 from 214 (out) to 229 (in) : 1599.05 Transmembrane helix 13 from 233 (in) to 248 (out) : 216.36 Transmembrane helix 14 from 252 (out) to 267 (in) : 924.861 Transmembrane helix 15 from 271 (in) to 286 (out) : -1012.37 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -645.522 Transmembrane helix 2 from 32 (in) to 47 (out) : 443.364 Transmembrane helix 3 from 51 (out) to 66 (in) : 301.277 Transmembrane helix 4 from 70 (in) to 85 (out) : -605.313 Transmembrane helix 5 from 89 (out) to 104 (in) : -375.782 Transmembrane helix 6 from 108 (in) to 123 (out) : 996.721 Transmembrane helix 7 from 127 (out) to 142 (in) : -556.708 Transmembrane helix 8 from 146 (in) to 161 (out) : 3554.47 Transmembrane helix 9 from 165 (out) to 180 (in) : -380.358 Transmembrane helix 10 from 184 (in) to 199 (out) : 2498.58 Transmembrane helix 11 from 203 (out) to 218 (in) : 515.033 Transmembrane helix 12 from 222 (in) to 237 (out) : 765.607 Transmembrane helix 13 from 241 (out) to 256 (in) : 838.279 Transmembrane helix 14 from 260 (in) to 275 (out) : 389.849 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -570.343 Transmembrane helix 2 from 33 (out) to 48 (in) : 538.562 Transmembrane helix 3 from 52 (in) to 67 (out) : 195.967 Transmembrane helix 4 from 74 (out) to 89 (in) : -578.327 Transmembrane helix 5 from 96 (in) to 111 (out) : 729.816 Transmembrane helix 6 from 115 (out) to 130 (in) : 65.137 Transmembrane helix 7 from 141 (in) to 160 (out) : 3818.33 Transmembrane helix 8 from 164 (out) to 179 (in) : -354.598 Transmembrane helix 9 from 183 (in) to 198 (out) : 2548.09 Transmembrane helix 10 from 202 (out) to 217 (in) : 386.092 Transmembrane helix 11 from 221 (in) to 236 (out) : 908.162 Transmembrane helix 12 from 240 (out) to 255 (in) : 783.604 Transmembrane helix 13 from 259 (in) to 274 (out) : 472.255 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.248 Transmembrane helix 2 from 44 (in) to 59 (out) : 985.231 Transmembrane helix 3 from 74 (out) to 89 (in) : -578.327 Transmembrane helix 4 from 96 (in) to 111 (out) : 729.816 Transmembrane helix 5 from 115 (out) to 130 (in) : 65.137 Transmembrane helix 6 from 141 (in) to 160 (out) : 3818.33 Transmembrane helix 7 from 164 (out) to 179 (in) : -354.598 Transmembrane helix 8 from 183 (in) to 198 (out) : 2548.09 Transmembrane helix 9 from 202 (out) to 217 (in) : 386.092 Transmembrane helix 10 from 221 (in) to 236 (out) : 908.162 Transmembrane helix 11 from 240 (out) to 255 (in) : 783.604 Transmembrane helix 12 from 259 (in) to 274 (out) : 472.255 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -570.343 Transmembrane helix 2 from 33 (out) to 48 (in) : 538.562 Transmembrane helix 3 from 52 (in) to 67 (out) : 195.967 Transmembrane helix 4 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 5 from 141 (in) to 160 (out) : 3818.33 Transmembrane helix 6 from 164 (out) to 179 (in) : -354.598 Transmembrane helix 7 from 183 (in) to 198 (out) : 2548.09 Transmembrane helix 8 from 202 (out) to 217 (in) : 386.092 Transmembrane helix 9 from 221 (in) to 236 (out) : 908.162 Transmembrane helix 10 from 240 (out) to 255 (in) : 783.604 Transmembrane helix 11 from 259 (in) to 274 (out) : 472.255 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.248 Transmembrane helix 2 from 44 (in) to 59 (out) : 985.231 Transmembrane helix 3 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 4 from 141 (in) to 160 (out) : 3818.33 Transmembrane helix 5 from 164 (out) to 179 (in) : -354.598 Transmembrane helix 6 from 183 (in) to 198 (out) : 2548.09 Transmembrane helix 7 from 202 (out) to 217 (in) : 386.092 Transmembrane helix 8 from 221 (in) to 236 (out) : 908.162 Transmembrane helix 9 from 240 (out) to 255 (in) : 783.604 Transmembrane helix 10 from 259 (in) to 274 (out) : 472.255 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -570.343 Transmembrane helix 2 from 33 (out) to 48 (in) : 538.562 Transmembrane helix 3 from 52 (in) to 67 (out) : 195.967 Transmembrane helix 4 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 5 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 6 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 7 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 8 from 239 (out) to 254 (in) : 711.793 Transmembrane helix 9 from 258 (in) to 273 (out) : 551.076 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.248 Transmembrane helix 2 from 44 (in) to 59 (out) : 985.231 Transmembrane helix 3 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 4 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 5 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 6 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 7 from 239 (out) to 254 (in) : 711.793 Transmembrane helix 8 from 258 (in) to 273 (out) : 551.076 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 37 (in) to 59 (out) : 1185.97 Transmembrane helix 2 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 3 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 4 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 5 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 6 from 239 (out) to 254 (in) : 711.793 Transmembrane helix 7 from 258 (in) to 273 (out) : 551.076 Score = 12.629794 Processing 6 helices: Transmembrane helix 1 from 37 (out) to 60 (in) : 1140.25 Transmembrane helix 2 from 103 (in) to 120 (out) : 1454.41 Transmembrane helix 3 from 141 (out) to 165 (in) : 3945.79 Transmembrane helix 4 from 181 (in) to 199 (out) : 2604.61 Transmembrane helix 5 from 211 (out) to 232 (in) : 1814.15 Transmembrane helix 6 from 242 (in) to 271 (out) : 1395.33 Score = 12.053509 Processing 5 helices: Transmembrane helix 1 from 37 (in) to 59 (out) : 1185.97 Transmembrane helix 2 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 3 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 4 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 5 from 211 (in) to 232 (out) : 1843.25 Score = 11.351914 Processing 4 helices: Transmembrane helix 1 from 103 (out) to 120 (in) : 1453.99 Transmembrane helix 2 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 3 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 4 from 211 (in) to 232 (out) : 1843.25 Score = 10.021914 Processing 3 helices: Transmembrane helix 1 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 2 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 3 from 211 (in) to 232 (out) : 1843.25 Score = 8.531495 Processing 2 helices: Transmembrane helix 1 from 141 (out) to 165 (in) : 3945.79 Transmembrane helix 2 from 181 (in) to 199 (out) : 2604.61 Score = 6.503118 Processing 15 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : -3163.84 Transmembrane helix 2 from 24 (in) to 39 (out) : -230.131 Transmembrane helix 3 from 43 (out) to 58 (in) : 989.943 Transmembrane helix 4 from 62 (in) to 77 (out) : -594.662 Transmembrane helix 5 from 81 (out) to 96 (in) : -694.169 Transmembrane helix 6 from 100 (in) to 115 (out) : 1211.67 Transmembrane helix 7 from 119 (out) to 134 (in) : -445.015 Transmembrane helix 8 from 138 (in) to 153 (out) : 2459.09 Transmembrane helix 9 from 157 (out) to 172 (in) : 714.125 Transmembrane helix 10 from 176 (in) to 191 (out) : 1515.85 Transmembrane helix 11 from 195 (out) to 210 (in) : 375.924 Transmembrane helix 12 from 214 (in) to 229 (out) : 1621.95 Transmembrane helix 13 from 233 (out) to 248 (in) : 223.14 Transmembrane helix 14 from 252 (in) to 267 (out) : 911.339 Transmembrane helix 15 from 271 (out) to 286 (in) : -1025.73 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -640.478 Transmembrane helix 2 from 32 (out) to 47 (in) : 432.636 Transmembrane helix 3 from 51 (in) to 66 (out) : 314.423 Transmembrane helix 4 from 70 (out) to 85 (in) : -585.387 Transmembrane helix 5 from 89 (in) to 104 (out) : -392.518 Transmembrane helix 6 from 108 (out) to 123 (in) : 997.779 Transmembrane helix 7 from 127 (in) to 142 (out) : -553.292 Transmembrane helix 8 from 146 (out) to 161 (in) : 3505.53 Transmembrane helix 9 from 165 (in) to 180 (out) : -392.142 Transmembrane helix 10 from 184 (out) to 199 (in) : 2519.02 Transmembrane helix 11 from 203 (in) to 218 (out) : 533.767 Transmembrane helix 12 from 222 (out) to 237 (in) : 757.793 Transmembrane helix 13 from 241 (in) to 256 (out) : 815.721 Transmembrane helix 14 from 260 (out) to 275 (in) : 408.751 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -575.757 Transmembrane helix 2 from 35 (in) to 50 (out) : 748.394 Transmembrane helix 3 from 54 (out) to 69 (in) : -20.323 Transmembrane helix 4 from 74 (in) to 89 (out) : -594.973 Transmembrane helix 5 from 96 (out) to 111 (in) : 738.084 Transmembrane helix 6 from 115 (in) to 130 (out) : 56.263 Transmembrane helix 7 from 141 (out) to 160 (in) : 3762.07 Transmembrane helix 8 from 164 (in) to 179 (out) : -362.502 Transmembrane helix 9 from 183 (out) to 198 (in) : 2569.11 Transmembrane helix 10 from 202 (in) to 217 (out) : 403.108 Transmembrane helix 11 from 221 (out) to 236 (in) : 900.738 Transmembrane helix 12 from 240 (in) to 255 (out) : 756.596 Transmembrane helix 13 from 259 (out) to 274 (in) : 488.645 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -570.343 Transmembrane helix 2 from 33 (out) to 48 (in) : 538.562 Transmembrane helix 3 from 52 (in) to 67 (out) : 195.967 Transmembrane helix 4 from 74 (out) to 89 (in) : -578.327 Transmembrane helix 5 from 96 (in) to 111 (out) : 729.816 Transmembrane helix 6 from 115 (out) to 130 (in) : 65.137 Transmembrane helix 7 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 8 from 181 (out) to 198 (in) : 2613.04 Transmembrane helix 9 from 202 (in) to 217 (out) : 403.108 Transmembrane helix 10 from 221 (out) to 236 (in) : 900.738 Transmembrane helix 11 from 240 (in) to 255 (out) : 756.596 Transmembrane helix 12 from 259 (out) to 274 (in) : 488.645 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.248 Transmembrane helix 2 from 44 (in) to 59 (out) : 985.231 Transmembrane helix 3 from 74 (out) to 89 (in) : -578.327 Transmembrane helix 4 from 96 (in) to 111 (out) : 729.816 Transmembrane helix 5 from 115 (out) to 130 (in) : 65.137 Transmembrane helix 6 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 7 from 181 (out) to 198 (in) : 2613.04 Transmembrane helix 8 from 202 (in) to 217 (out) : 403.108 Transmembrane helix 9 from 221 (out) to 236 (in) : 900.738 Transmembrane helix 10 from 240 (in) to 255 (out) : 756.596 Transmembrane helix 11 from 259 (out) to 274 (in) : 488.645 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -570.343 Transmembrane helix 2 from 33 (out) to 48 (in) : 538.562 Transmembrane helix 3 from 52 (in) to 67 (out) : 195.967 Transmembrane helix 4 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 5 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 6 from 181 (out) to 198 (in) : 2613.04 Transmembrane helix 7 from 202 (in) to 217 (out) : 403.108 Transmembrane helix 8 from 221 (out) to 236 (in) : 900.738 Transmembrane helix 9 from 240 (in) to 255 (out) : 756.596 Transmembrane helix 10 from 259 (out) to 274 (in) : 488.645 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.248 Transmembrane helix 2 from 44 (in) to 59 (out) : 985.231 Transmembrane helix 3 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 4 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 5 from 181 (out) to 198 (in) : 2613.04 Transmembrane helix 6 from 202 (in) to 217 (out) : 403.108 Transmembrane helix 7 from 221 (out) to 236 (in) : 900.738 Transmembrane helix 8 from 240 (in) to 255 (out) : 756.596 Transmembrane helix 9 from 259 (out) to 274 (in) : 488.645 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 37 (in) to 59 (out) : 1185.97 Transmembrane helix 2 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 3 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 4 from 181 (out) to 198 (in) : 2613.04 Transmembrane helix 5 from 202 (in) to 217 (out) : 403.108 Transmembrane helix 6 from 221 (out) to 236 (in) : 900.738 Transmembrane helix 7 from 240 (in) to 255 (out) : 756.596 Transmembrane helix 8 from 259 (out) to 274 (in) : 488.645 Score = 12.081985 Processing 7 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -177.248 Transmembrane helix 2 from 44 (in) to 59 (out) : 985.231 Transmembrane helix 3 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 4 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 5 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 6 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 7 from 242 (out) to 269 (in) : 1429.36 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 37 (in) to 59 (out) : 1185.97 Transmembrane helix 2 from 102 (out) to 120 (in) : 1453.09 Transmembrane helix 3 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 4 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 5 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 6 from 242 (out) to 269 (in) : 1429.36 Score = 12.880320 Processing 5 helices: Transmembrane helix 1 from 103 (out) to 120 (in) : 1453.99 Transmembrane helix 2 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 3 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 4 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 5 from 242 (out) to 269 (in) : 1429.36 Score = 11.550320 Processing 4 helices: Transmembrane helix 1 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 2 from 181 (out) to 199 (in) : 2635.19 Transmembrane helix 3 from 211 (in) to 232 (out) : 1843.25 Transmembrane helix 4 from 242 (out) to 269 (in) : 1429.36 Score = 10.059900 Processing 3 helices: Transmembrane helix 1 from 141 (out) to 165 (in) : 3945.79 Transmembrane helix 2 from 181 (in) to 199 (out) : 2604.61 Transmembrane helix 3 from 211 (out) to 232 (in) : 1814.15 Score = 8.334705 Processing 2 helices: Transmembrane helix 1 from 141 (in) to 165 (out) : 4023.21 Transmembrane helix 2 from 181 (out) to 199 (in) : 2635.19 Score = 6.705683 Summary of topology analysis: 1 helix (+) : Score = 4.02321 1 helix (-) : Score = 3.94579 2 helices (+) : Score = 6.70568 2 helices (-) : Score = 6.50312 3 helices (+) : Score = 8.5315 3 helices (-) : Score = 8.33471 4 helices (+) : Score = 10.0599 4 helices (-) : Score = 10.0219 5 helices (+) : Score = 11.3519 5 helices (-) : Score = 11.5503 6 helices (+) : Score = 12.8803 6 helices (-) : Score = 12.0535 7 helices (+) : Score = 12.6298 7 helices (-) : Score = -100000 8 helices (+) : Score = 12.082 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 5.916 Topology: 37-59,102-120,141-165,181-199,211-232,242-269 Re-entrant helices: Not detected. Helix count: 6 N-terminal: in Score: 12.8803