BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (136 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AAW7 Inner membrane protein ybhQ n=105 Tax=Enterobact...   270   1e-71
UniRef50_B2VBW0 Inner membrane protein YbhQ n=4 Tax=Enterobacter...   100   2e-20

>UniRef50_P0AAW7 Inner membrane protein ybhQ n=105 Tax=Enterobacteriaceae
           RepID=YBHQ_ECO57
          Length = 136

 Score =  270 bits (689), Expect = 1e-71,   Method: Compositional matrix adjust.
 Identities = 136/136 (100%), Positives = 136/136 (100%)

Query: 1   MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR 60
           MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR
Sbjct: 1   MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR 60

Query: 61  HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA 120
           HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA
Sbjct: 61  HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA 120

Query: 121 KDKKLHHLTSKHRVRR 136
           KDKKLHHLTSKHRVRR
Sbjct: 121 KDKKLHHLTSKHRVRR 136


>UniRef50_B2VBW0 Inner membrane protein YbhQ n=4 Tax=Enterobacteriaceae
           RepID=B2VBW0_ERWT9
          Length = 132

 Score =  100 bits (249), Expect = 2e-20,   Method: Compositional matrix adjust.
 Identities = 64/136 (47%), Positives = 89/136 (65%), Gaps = 6/136 (4%)

Query: 1   MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR 60
           MKW  R+++ TG +C  I LHLL++A LV GW  + L+ V   L ALY      ML+ QR
Sbjct: 1   MKWSHRIQIVTGQNCVHIALHLLLIAALVWGWKHQALLQVCTILLALYAGVFTAMLLTQR 60

Query: 61  HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA 120
            P  R R + D LE++TTT+YFGAA++VL +LSRV+ NN LL   G+ +L GPA+VSLL 
Sbjct: 61  LP--RLRRIGDFLEDVTTTYYFGAAMMVLLVLSRVIHNNLLLGAFGVMMLTGPALVSLLV 118

Query: 121 KDKKLHHLTSKHRVRR 136
           K++    +  +HR +R
Sbjct: 119 KER----VQPQHRGQR 130


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AAW7 Inner membrane protein ybhQ n=105 Tax=Enterobact...   174   8e-43
UniRef50_B2VBW0 Inner membrane protein YbhQ n=4 Tax=Enterobacter...   150   9e-36

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AAW7 Inner membrane protein ybhQ n=105 Tax=Enterobacteriaceae
           RepID=YBHQ_ECO57
          Length = 136

 Score =  174 bits (441), Expect = 8e-43,   Method: Composition-based stats.
 Identities = 136/136 (100%), Positives = 136/136 (100%)

Query: 1   MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR 60
           MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR
Sbjct: 1   MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR 60

Query: 61  HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA 120
           HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA
Sbjct: 61  HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA 120

Query: 121 KDKKLHHLTSKHRVRR 136
           KDKKLHHLTSKHRVRR
Sbjct: 121 KDKKLHHLTSKHRVRR 136


>UniRef50_B2VBW0 Inner membrane protein YbhQ n=4 Tax=Enterobacteriaceae
           RepID=B2VBW0_ERWT9
          Length = 132

 Score =  150 bits (380), Expect = 9e-36,   Method: Composition-based stats.
 Identities = 64/136 (47%), Positives = 89/136 (65%), Gaps = 6/136 (4%)

Query: 1   MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGLCALYCVTVVMMLVFQR 60
           MKW  R+++ TG +C  I LHLL++A LV GW  + L+ V   L ALY      ML+ QR
Sbjct: 1   MKWSHRIQIVTGQNCVHIALHLLLIAALVWGWKHQALLQVCTILLALYAGVFTAMLLTQR 60

Query: 61  HPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLLAIAGLAILAGPAVVSLLA 120
            P  R R + D LE++TTT+YFGAA++VL +LSRV+ NN LL   G+ +L GPA+VSLL 
Sbjct: 61  LP--RLRRIGDFLEDVTTTYYFGAAMMVLLVLSRVIHNNLLLGAFGVMMLTGPALVSLLV 118

Query: 121 KDKKLHHLTSKHRVRR 136
           K++    +  +HR +R
Sbjct: 119 KER----VQPQHRGQR 130


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.332    0.143    0.442 

Lambda     K      H
   0.267   0.0432    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 590,536,715
Number of Sequences: 3077464
Number of extensions: 22503634
Number of successful extensions: 94907
Number of sequences better than 1.0e-01: 4
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 94900
Number of HSP's gapped (non-prelim): 6
length of query: 136
length of database: 1,040,396,356
effective HSP length: 101
effective length of query: 35
effective length of database: 729,572,492
effective search space: 25535037220
effective search space used: 25535037220
T: 11
A: 40
X1: 16 ( 7.7 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 87 (38.0 bits)