BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (78 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P75687 Uncharacterized protein ykgI n=76 Tax=Enterobact...   154   8e-37
UniRef50_B5PFA9 Putative uncharacterized protein n=1 Tax=Salmone...    42   0.007
UniRef50_B7NH61 Putative uncharacterized protein n=2 Tax=Enterob...    39   0.058

>UniRef50_P75687 Uncharacterized protein ykgI n=76 Tax=Enterobacteriaceae
          RepID=YKGI_ECOLI
          Length = 78

 Score =  154 bits (389), Expect = 8e-37,   Method: Compositional matrix adjust.
 Identities = 78/78 (100%), Positives = 78/78 (100%)

Query: 1  MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWK 60
          MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWK
Sbjct: 1  MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWK 60

Query: 61 VTSMRIDNNSTATAVLYK 78
          VTSMRIDNNSTATAVLYK
Sbjct: 61 VTSMRIDNNSTATAVLYK 78


>UniRef50_B5PFA9 Putative uncharacterized protein n=1 Tax=Salmonella enterica
          subsp. enterica serovar Weltevreden str. HI_N05-537
          RepID=B5PFA9_SALET
          Length = 87

 Score = 42.0 bits (97), Expect = 0.007,   Method: Compositional matrix adjust.
 Identities = 19/49 (38%), Positives = 30/49 (61%)

Query: 30 HISVSSVSASPTVLEDTIADIARKYNASSWKVTSMRIDNNSTATAVLYK 78
          H+SVSS++ +P  +   + +      A  +++TS  IDN S ATA+LYK
Sbjct: 39 HVSVSSINTNPESIIQLLQNKTEASGAHYYRITSFHIDNQSHATAILYK 87


>UniRef50_B7NH61 Putative uncharacterized protein n=2 Tax=Enterobacteriaceae
          RepID=B7NH61_ECOLU
          Length = 70

 Score = 38.5 bits (88), Expect = 0.058,   Method: Compositional matrix adjust.
 Identities = 23/50 (46%), Positives = 31/50 (62%), Gaps = 3/50 (6%)

Query: 32 SVSSVSASPTVL---EDTIADIARKYNASSWKVTSMRIDNNSTATAVLYK 78
          S  SV+A+ T L   E  IA  A++ NAS +K+TS R+ N  T +A LYK
Sbjct: 21 SAQSVTATGTTLDSAEAVIAQKAKEINASEYKITSARMGNTVTMSAELYK 70


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P75687 Uncharacterized protein ykgI n=76 Tax=Enterobact...   122   2e-27

Sequences not found previously or not previously below threshold:

UniRef50_B5PFA9 Putative uncharacterized protein n=1 Tax=Salmone...    39   0.039


CONVERGED!
>UniRef50_P75687 Uncharacterized protein ykgI n=76 Tax=Enterobacteriaceae
          RepID=YKGI_ECOLI
          Length = 78

 Score =  122 bits (307), Expect = 2e-27,   Method: Composition-based stats.
 Identities = 78/78 (100%), Positives = 78/78 (100%)

Query: 1  MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWK 60
          MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWK
Sbjct: 1  MFKKSVLFATLLSGVMAFSTNADDKIILKHISVSSVSASPTVLEDTIADIARKYNASSWK 60

Query: 61 VTSMRIDNNSTATAVLYK 78
          VTSMRIDNNSTATAVLYK
Sbjct: 61 VTSMRIDNNSTATAVLYK 78


>UniRef50_B5PFA9 Putative uncharacterized protein n=1 Tax=Salmonella enterica
          subsp. enterica serovar Weltevreden str. HI_N05-537
          RepID=B5PFA9_SALET
          Length = 87

 Score = 39.4 bits (90), Expect = 0.039,   Method: Composition-based stats.
 Identities = 19/51 (37%), Positives = 31/51 (60%)

Query: 28 LKHISVSSVSASPTVLEDTIADIARKYNASSWKVTSMRIDNNSTATAVLYK 78
          + H+SVSS++ +P  +   + +      A  +++TS  IDN S ATA+LYK
Sbjct: 37 VAHVSVSSINTNPESIIQLLQNKTEASGAHYYRITSFHIDNQSHATAILYK 87


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.315    0.123    0.327 

Lambda     K      H
   0.267   0.0388    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 241,883,571
Number of Sequences: 3077464
Number of extensions: 6404930
Number of successful extensions: 21825
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 21821
Number of HSP's gapped (non-prelim): 6
length of query: 78
length of database: 1,040,396,356
effective HSP length: 49
effective length of query: 29
effective length of database: 889,600,620
effective search space: 25798417980
effective search space used: 25798417980
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 87 (38.2 bits)