Processing 1 helix: Transmembrane helix 1 from 216 (in) to 231 (out) : 431.711 Score = 0.353078 Processing 1 helix: Transmembrane helix 1 from 215 (out) to 230 (in) : 447.399 Score = 0.528011 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 89 (in) to 104 (out) : 234.992 Transmembrane helix 6 from 108 (out) to 123 (in) : -479.715 Transmembrane helix 7 from 127 (in) to 142 (out) : -411.695 Transmembrane helix 8 from 146 (out) to 161 (in) : -518.48 Transmembrane helix 9 from 165 (in) to 180 (out) : -634.356 Transmembrane helix 10 from 184 (out) to 199 (in) : -723.79 Transmembrane helix 11 from 203 (in) to 218 (out) : -174.718 Transmembrane helix 12 from 222 (out) to 237 (in) : 73.185 Transmembrane helix 13 from 241 (in) to 256 (out) : -686.31 Transmembrane helix 14 from 260 (out) to 275 (in) : -462.659 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -156.96 Transmembrane helix 2 from 23 (in) to 38 (out) : -101.743 Transmembrane helix 3 from 42 (out) to 57 (in) : -163.908 Transmembrane helix 4 from 61 (in) to 76 (out) : -361.012 Transmembrane helix 5 from 80 (out) to 95 (in) : -502.677 Transmembrane helix 6 from 99 (in) to 114 (out) : 163.068 Transmembrane helix 7 from 118 (out) to 133 (in) : -637.884 Transmembrane helix 8 from 137 (in) to 152 (out) : -397.345 Transmembrane helix 9 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 10 from 194 (in) to 209 (out) : -621.603 Transmembrane helix 11 from 213 (out) to 228 (in) : 430.927 Transmembrane helix 12 from 232 (in) to 247 (out) : -502.242 Transmembrane helix 13 from 260 (out) to 275 (in) : -462.659 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 90 (in) to 105 (out) : 276.599 Transmembrane helix 6 from 109 (out) to 124 (in) : -514.91 Transmembrane helix 7 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 8 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 9 from 194 (in) to 209 (out) : -621.603 Transmembrane helix 10 from 213 (out) to 228 (in) : 430.927 Transmembrane helix 11 from 232 (in) to 247 (out) : -502.242 Transmembrane helix 12 from 260 (out) to 275 (in) : -462.659 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -156.96 Transmembrane helix 2 from 23 (in) to 38 (out) : -101.743 Transmembrane helix 3 from 42 (out) to 57 (in) : -163.908 Transmembrane helix 4 from 61 (in) to 76 (out) : -361.012 Transmembrane helix 5 from 93 (out) to 108 (in) : 328.706 Transmembrane helix 6 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 7 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 8 from 194 (in) to 209 (out) : -621.603 Transmembrane helix 9 from 213 (out) to 228 (in) : 430.927 Transmembrane helix 10 from 232 (in) to 247 (out) : -502.242 Transmembrane helix 11 from 260 (out) to 275 (in) : -462.659 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 90 (in) to 105 (out) : 276.599 Transmembrane helix 6 from 109 (out) to 124 (in) : -514.91 Transmembrane helix 7 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 8 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 9 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 10 from 224 (out) to 239 (in) : -62.322 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -156.96 Transmembrane helix 2 from 23 (in) to 38 (out) : -101.743 Transmembrane helix 3 from 42 (out) to 57 (in) : -163.908 Transmembrane helix 4 from 61 (in) to 76 (out) : -361.012 Transmembrane helix 5 from 93 (out) to 108 (in) : 328.706 Transmembrane helix 6 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 7 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 8 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 9 from 224 (out) to 239 (in) : -62.322 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 6 from 131 (out) to 146 (in) : -334.512 Transmembrane helix 7 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 8 from 224 (out) to 239 (in) : -62.322 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -140.704 Transmembrane helix 2 from 25 (in) to 40 (out) : -103.621 Transmembrane helix 3 from 44 (out) to 59 (in) : -176.276 Transmembrane helix 4 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 5 from 131 (out) to 146 (in) : -334.512 Transmembrane helix 6 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 7 from 224 (out) to 239 (in) : -62.322 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 6 from 215 (out) to 230 (in) : 447.399 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -140.704 Transmembrane helix 2 from 25 (in) to 40 (out) : -103.621 Transmembrane helix 3 from 44 (out) to 59 (in) : -176.276 Transmembrane helix 4 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 5 from 215 (out) to 230 (in) : 447.399 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -112.276 Transmembrane helix 2 from 33 (out) to 48 (in) : -116.371 Transmembrane helix 3 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 4 from 215 (out) to 230 (in) : 447.399 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 2 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 3 from 215 (out) to 230 (in) : 447.399 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 2 from 215 (out) to 230 (in) : 447.399 Score = 0.873605 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -156.96 Transmembrane helix 2 from 23 (in) to 38 (out) : -101.743 Transmembrane helix 3 from 42 (out) to 57 (in) : -163.908 Transmembrane helix 4 from 61 (in) to 76 (out) : -361.012 Transmembrane helix 5 from 80 (out) to 95 (in) : -502.677 Transmembrane helix 6 from 99 (in) to 114 (out) : 163.068 Transmembrane helix 7 from 118 (out) to 133 (in) : -637.884 Transmembrane helix 8 from 137 (in) to 152 (out) : -397.345 Transmembrane helix 9 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 10 from 183 (in) to 198 (out) : -703.144 Transmembrane helix 11 from 203 (out) to 218 (in) : -190.182 Transmembrane helix 12 from 222 (in) to 237 (out) : 82.715 Transmembrane helix 13 from 241 (out) to 256 (in) : -683.99 Transmembrane helix 14 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 90 (in) to 105 (out) : 276.599 Transmembrane helix 6 from 109 (out) to 124 (in) : -514.91 Transmembrane helix 7 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 8 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 9 from 183 (in) to 198 (out) : -703.144 Transmembrane helix 10 from 203 (out) to 218 (in) : -190.182 Transmembrane helix 11 from 222 (in) to 237 (out) : 82.715 Transmembrane helix 12 from 241 (out) to 256 (in) : -683.99 Transmembrane helix 13 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -156.96 Transmembrane helix 2 from 23 (in) to 38 (out) : -101.743 Transmembrane helix 3 from 42 (out) to 57 (in) : -163.908 Transmembrane helix 4 from 61 (in) to 76 (out) : -361.012 Transmembrane helix 5 from 80 (out) to 95 (in) : -502.677 Transmembrane helix 6 from 99 (in) to 114 (out) : 163.068 Transmembrane helix 7 from 118 (out) to 133 (in) : -637.884 Transmembrane helix 8 from 137 (in) to 152 (out) : -397.345 Transmembrane helix 9 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 10 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 11 from 224 (out) to 239 (in) : -62.322 Transmembrane helix 12 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 90 (in) to 105 (out) : 276.599 Transmembrane helix 6 from 109 (out) to 124 (in) : -514.91 Transmembrane helix 7 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 8 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 9 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 10 from 224 (out) to 239 (in) : -62.322 Transmembrane helix 11 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -156.96 Transmembrane helix 2 from 23 (in) to 38 (out) : -101.743 Transmembrane helix 3 from 42 (out) to 57 (in) : -163.908 Transmembrane helix 4 from 61 (in) to 76 (out) : -361.012 Transmembrane helix 5 from 93 (out) to 108 (in) : 328.706 Transmembrane helix 6 from 133 (in) to 148 (out) : -347.848 Transmembrane helix 7 from 156 (out) to 171 (in) : -419.395 Transmembrane helix 8 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 9 from 224 (out) to 239 (in) : -62.322 Transmembrane helix 10 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 6 from 131 (out) to 146 (in) : -334.512 Transmembrane helix 7 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 8 from 224 (out) to 239 (in) : -62.322 Transmembrane helix 9 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -140.704 Transmembrane helix 2 from 25 (in) to 40 (out) : -103.621 Transmembrane helix 3 from 44 (out) to 59 (in) : -176.276 Transmembrane helix 4 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 5 from 131 (out) to 146 (in) : -334.512 Transmembrane helix 6 from 205 (in) to 220 (out) : 1.66097 Transmembrane helix 7 from 224 (out) to 239 (in) : -62.322 Transmembrane helix 8 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -155.34 Transmembrane helix 2 from 23 (out) to 38 (in) : -102.557 Transmembrane helix 3 from 42 (in) to 57 (out) : -166.892 Transmembrane helix 4 from 61 (out) to 76 (in) : -362.988 Transmembrane helix 5 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 6 from 215 (out) to 230 (in) : 447.399 Transmembrane helix 7 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -140.704 Transmembrane helix 2 from 25 (in) to 40 (out) : -103.621 Transmembrane helix 3 from 44 (out) to 59 (in) : -176.276 Transmembrane helix 4 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 5 from 215 (out) to 230 (in) : 447.399 Transmembrane helix 6 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 14 (in) to 29 (out) : -112.276 Transmembrane helix 2 from 33 (out) to 48 (in) : -116.371 Transmembrane helix 3 from 93 (in) to 108 (out) : 345.594 Transmembrane helix 4 from 215 (out) to 230 (in) : 447.399 Transmembrane helix 5 from 260 (in) to 276 (out) : -445.848 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 14 (out) to 29 (in) : -112.924 Transmembrane helix 2 from 33 (in) to 48 (out) : -115.229 Transmembrane helix 3 from 93 (out) to 108 (in) : 328.706 Transmembrane helix 4 from 216 (in) to 231 (out) : 431.711 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : -108.567 Transmembrane helix 2 from 93 (out) to 108 (in) : 328.706 Transmembrane helix 3 from 216 (in) to 231 (out) : 431.711 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 93 (out) to 108 (in) : 328.706 Transmembrane helix 2 from 216 (in) to 231 (out) : 431.711 Score = 0.681784 Summary of topology analysis: 1 helix (+) : Score = 0.353078 1 helix (-) : Score = 0.528011 2 helices (+) : Score = 0.873605 2 helices (-) : Score = 0.681784 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 93-108,215-230 Re-entrant helices: Not detected. Helix count: 2 N-terminal: in Score: 0.873605