Processing 1 helix: Transmembrane helix 1 from 82 (in) to 97 (out) : -396.162 Score = -0.396162 Processing 1 helix: Transmembrane helix 1 from 83 (out) to 98 (in) : -393.132 Score = -0.393132 Processing 10 helices: Transmembrane helix 1 from 17 (in) to 32 (out) : -1776.83 Transmembrane helix 2 from 36 (out) to 51 (in) : -1463.68 Transmembrane helix 3 from 55 (in) to 70 (out) : -1151.46 Transmembrane helix 4 from 74 (out) to 89 (in) : -774.84 Transmembrane helix 5 from 93 (in) to 108 (out) : -943.967 Transmembrane helix 6 from 112 (out) to 127 (in) : -908.293 Transmembrane helix 7 from 131 (in) to 146 (out) : -800.925 Transmembrane helix 8 from 150 (out) to 165 (in) : -1510.09 Transmembrane helix 9 from 169 (in) to 184 (out) : -1099.92 Transmembrane helix 10 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 22 (out) to 37 (in) : -1002.65 Transmembrane helix 2 from 46 (in) to 61 (out) : -1181.3 Transmembrane helix 3 from 66 (out) to 81 (in) : -1077.98 Transmembrane helix 4 from 85 (in) to 100 (out) : -435.835 Transmembrane helix 5 from 112 (out) to 127 (in) : -908.293 Transmembrane helix 6 from 131 (in) to 146 (out) : -800.925 Transmembrane helix 7 from 150 (out) to 165 (in) : -1510.09 Transmembrane helix 8 from 169 (in) to 184 (out) : -1099.92 Transmembrane helix 9 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -992.247 Transmembrane helix 2 from 48 (out) to 63 (in) : -1173.14 Transmembrane helix 3 from 67 (in) to 82 (out) : -1044.13 Transmembrane helix 4 from 86 (out) to 101 (in) : -467.799 Transmembrane helix 5 from 114 (in) to 129 (out) : -771.923 Transmembrane helix 6 from 133 (out) to 148 (in) : -939.185 Transmembrane helix 7 from 169 (in) to 184 (out) : -1099.92 Transmembrane helix 8 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 22 (out) to 37 (in) : -1002.65 Transmembrane helix 2 from 46 (in) to 61 (out) : -1181.3 Transmembrane helix 3 from 66 (out) to 81 (in) : -1077.98 Transmembrane helix 4 from 85 (in) to 100 (out) : -435.835 Transmembrane helix 5 from 122 (out) to 137 (in) : -441.854 Transmembrane helix 6 from 169 (in) to 184 (out) : -1099.92 Transmembrane helix 7 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -992.247 Transmembrane helix 2 from 48 (out) to 63 (in) : -1173.14 Transmembrane helix 3 from 67 (in) to 82 (out) : -1044.13 Transmembrane helix 4 from 86 (out) to 101 (in) : -467.799 Transmembrane helix 5 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 6 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 22 (out) to 37 (in) : -1002.65 Transmembrane helix 2 from 67 (in) to 82 (out) : -1044.13 Transmembrane helix 3 from 86 (out) to 101 (in) : -467.799 Transmembrane helix 4 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 5 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -992.247 Transmembrane helix 2 from 83 (out) to 98 (in) : -393.132 Transmembrane helix 3 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 4 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 83 (out) to 98 (in) : -393.132 Transmembrane helix 2 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 3 from 188 (out) to 203 (in) : -506.819 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 82 (in) to 97 (out) : -396.162 Transmembrane helix 2 from 122 (out) to 137 (in) : -441.854 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 17 (out) to 32 (in) : -1785.97 Transmembrane helix 2 from 36 (in) to 51 (out) : -1465.71 Transmembrane helix 3 from 55 (out) to 70 (in) : -1150.34 Transmembrane helix 4 from 74 (in) to 89 (out) : -762.46 Transmembrane helix 5 from 93 (out) to 108 (in) : -946.833 Transmembrane helix 6 from 112 (in) to 127 (out) : -904.607 Transmembrane helix 7 from 131 (out) to 146 (in) : -803.975 Transmembrane helix 8 from 150 (in) to 165 (out) : -1510.81 Transmembrane helix 9 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 10 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -992.247 Transmembrane helix 2 from 48 (out) to 63 (in) : -1173.14 Transmembrane helix 3 from 67 (in) to 82 (out) : -1044.13 Transmembrane helix 4 from 86 (out) to 101 (in) : -467.799 Transmembrane helix 5 from 112 (in) to 127 (out) : -904.607 Transmembrane helix 6 from 131 (out) to 146 (in) : -803.975 Transmembrane helix 7 from 150 (in) to 165 (out) : -1510.81 Transmembrane helix 8 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 9 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 22 (out) to 37 (in) : -1002.65 Transmembrane helix 2 from 46 (in) to 61 (out) : -1181.3 Transmembrane helix 3 from 66 (out) to 81 (in) : -1077.98 Transmembrane helix 4 from 85 (in) to 100 (out) : -435.835 Transmembrane helix 5 from 112 (out) to 127 (in) : -908.293 Transmembrane helix 6 from 131 (in) to 146 (out) : -800.925 Transmembrane helix 7 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 8 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -992.247 Transmembrane helix 2 from 48 (out) to 63 (in) : -1173.14 Transmembrane helix 3 from 67 (in) to 82 (out) : -1044.13 Transmembrane helix 4 from 86 (out) to 101 (in) : -467.799 Transmembrane helix 5 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 6 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 7 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 22 (out) to 37 (in) : -1002.65 Transmembrane helix 2 from 67 (in) to 82 (out) : -1044.13 Transmembrane helix 3 from 86 (out) to 101 (in) : -467.799 Transmembrane helix 4 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 5 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 6 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -992.247 Transmembrane helix 2 from 83 (out) to 98 (in) : -393.132 Transmembrane helix 3 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 4 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 5 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 83 (out) to 98 (in) : -393.132 Transmembrane helix 2 from 122 (in) to 137 (out) : -430.846 Transmembrane helix 3 from 169 (out) to 184 (in) : -1090.68 Transmembrane helix 4 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 82 (in) to 97 (out) : -396.162 Transmembrane helix 2 from 122 (out) to 137 (in) : -441.854 Transmembrane helix 3 from 188 (in) to 203 (out) : -528.081 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 83 (out) to 98 (in) : -393.132 Transmembrane helix 2 from 122 (in) to 137 (out) : -430.846 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.396162 1 helix (-) : Score = -0.393132 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 Results: Signal peptide: 1-16 Signal score: 30.966 Topology: 83-98 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.393132