Processing 1 helix: Transmembrane helix 1 from 175 (in) to 190 (out) : 1310.94 Score = 1.310943 Processing 1 helix: Transmembrane helix 1 from 175 (out) to 190 (in) : 1311.26 Score = 1.311257 Processing 15 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 23 (out) to 38 (in) : -595.598 Transmembrane helix 3 from 52 (in) to 67 (out) : -857.704 Transmembrane helix 4 from 71 (out) to 86 (in) : -281.389 Transmembrane helix 5 from 90 (in) to 105 (out) : -130.411 Transmembrane helix 6 from 109 (out) to 124 (in) : -111.035 Transmembrane helix 7 from 128 (in) to 143 (out) : -831.943 Transmembrane helix 8 from 150 (out) to 165 (in) : -761.238 Transmembrane helix 9 from 170 (in) to 185 (out) : 806.507 Transmembrane helix 10 from 189 (out) to 204 (in) : -293.943 Transmembrane helix 11 from 208 (in) to 223 (out) : -283.83 Transmembrane helix 12 from 227 (out) to 242 (in) : -576.179 Transmembrane helix 13 from 246 (in) to 261 (out) : -256.053 Transmembrane helix 14 from 265 (out) to 280 (in) : -128.084 Transmembrane helix 15 from 284 (in) to 299 (out) : -770.592 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 23 (in) to 38 (out) : -596.802 Transmembrane helix 3 from 52 (out) to 67 (in) : -862.096 Transmembrane helix 4 from 71 (in) to 86 (out) : -272.911 Transmembrane helix 5 from 90 (out) to 105 (in) : -138.489 Transmembrane helix 6 from 109 (in) to 124 (out) : -109.165 Transmembrane helix 7 from 128 (out) to 143 (in) : -821.057 Transmembrane helix 8 from 150 (in) to 165 (out) : -764.762 Transmembrane helix 9 from 174 (out) to 189 (in) : 1289.22 Transmembrane helix 10 from 193 (in) to 208 (out) : -484.698 Transmembrane helix 11 from 212 (out) to 227 (in) : -467.185 Transmembrane helix 12 from 233 (in) to 248 (out) : -509.902 Transmembrane helix 13 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 14 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 23 (out) to 38 (in) : -595.598 Transmembrane helix 3 from 65 (in) to 80 (out) : -349.538 Transmembrane helix 4 from 84 (out) to 99 (in) : -75.539 Transmembrane helix 5 from 103 (in) to 118 (out) : -214.7 Transmembrane helix 6 from 122 (out) to 137 (in) : -555.35 Transmembrane helix 7 from 150 (in) to 165 (out) : -764.762 Transmembrane helix 8 from 174 (out) to 189 (in) : 1289.22 Transmembrane helix 9 from 193 (in) to 208 (out) : -484.698 Transmembrane helix 10 from 212 (out) to 227 (in) : -467.185 Transmembrane helix 11 from 233 (in) to 248 (out) : -509.902 Transmembrane helix 12 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 13 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 23 (in) to 38 (out) : -596.802 Transmembrane helix 3 from 68 (out) to 83 (in) : -303.082 Transmembrane helix 4 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 5 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 6 from 150 (in) to 165 (out) : -764.762 Transmembrane helix 7 from 174 (out) to 189 (in) : 1289.22 Transmembrane helix 8 from 193 (in) to 208 (out) : -484.698 Transmembrane helix 9 from 212 (out) to 227 (in) : -467.185 Transmembrane helix 10 from 233 (in) to 248 (out) : -509.902 Transmembrane helix 11 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 12 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 23 (out) to 38 (in) : -595.598 Transmembrane helix 3 from 65 (in) to 80 (out) : -349.538 Transmembrane helix 4 from 84 (out) to 99 (in) : -75.539 Transmembrane helix 5 from 103 (in) to 118 (out) : -214.7 Transmembrane helix 6 from 122 (out) to 137 (in) : -555.35 Transmembrane helix 7 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 8 from 204 (out) to 219 (in) : -208.966 Transmembrane helix 9 from 233 (in) to 248 (out) : -509.902 Transmembrane helix 10 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 11 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 23 (in) to 38 (out) : -596.802 Transmembrane helix 3 from 68 (out) to 83 (in) : -303.082 Transmembrane helix 4 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 5 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 6 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 7 from 204 (out) to 219 (in) : -208.966 Transmembrane helix 8 from 233 (in) to 248 (out) : -509.902 Transmembrane helix 9 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 10 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 68 (out) to 83 (in) : -303.082 Transmembrane helix 3 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 4 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 5 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 6 from 204 (out) to 219 (in) : -208.966 Transmembrane helix 7 from 233 (in) to 248 (out) : -509.902 Transmembrane helix 8 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 9 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 68 (in) to 83 (out) : -290.018 Transmembrane helix 3 from 87 (out) to 102 (in) : -76.549 Transmembrane helix 4 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 5 from 175 (out) to 190 (in) : 1311.26 Transmembrane helix 6 from 205 (in) to 220 (out) : -218.443 Transmembrane helix 7 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 8 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 85 (out) to 100 (in) : -69.428 Transmembrane helix 3 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 4 from 175 (out) to 190 (in) : 1311.26 Transmembrane helix 5 from 205 (in) to 220 (out) : -218.443 Transmembrane helix 6 from 252 (out) to 267 (in) : -167.097 Transmembrane helix 7 from 271 (in) to 286 (out) : -184.119 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 3 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 4 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 5 from 204 (out) to 219 (in) : -208.966 Transmembrane helix 6 from 266 (in) to 281 (out) : -128.947 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 85 (out) to 100 (in) : -69.428 Transmembrane helix 3 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 4 from 175 (out) to 190 (in) : 1311.26 Transmembrane helix 5 from 266 (in) to 281 (out) : -128.947 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 3 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 4 from 175 (in) to 190 (out) : 1310.94 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 3 from 175 (in) to 190 (out) : 1310.94 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 175 (in) to 190 (out) : 1310.94 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 23 (in) to 38 (out) : -596.802 Transmembrane helix 3 from 52 (out) to 67 (in) : -862.096 Transmembrane helix 4 from 71 (in) to 86 (out) : -272.911 Transmembrane helix 5 from 90 (out) to 105 (in) : -138.489 Transmembrane helix 6 from 109 (in) to 124 (out) : -109.165 Transmembrane helix 7 from 128 (out) to 143 (in) : -821.057 Transmembrane helix 8 from 150 (in) to 165 (out) : -764.762 Transmembrane helix 9 from 170 (out) to 185 (in) : 809.893 Transmembrane helix 10 from 189 (in) to 204 (out) : -303.457 Transmembrane helix 11 from 208 (out) to 223 (in) : -287.07 Transmembrane helix 12 from 227 (in) to 242 (out) : -583.321 Transmembrane helix 13 from 246 (out) to 261 (in) : -265.147 Transmembrane helix 14 from 265 (in) to 280 (out) : -129.416 Transmembrane helix 15 from 284 (out) to 299 (in) : -770.508 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 23 (out) to 38 (in) : -595.598 Transmembrane helix 3 from 52 (in) to 67 (out) : -857.704 Transmembrane helix 4 from 71 (out) to 86 (in) : -281.389 Transmembrane helix 5 from 90 (in) to 105 (out) : -130.411 Transmembrane helix 6 from 109 (out) to 124 (in) : -111.035 Transmembrane helix 7 from 128 (in) to 143 (out) : -831.943 Transmembrane helix 8 from 150 (out) to 165 (in) : -761.238 Transmembrane helix 9 from 174 (in) to 189 (out) : 1289.39 Transmembrane helix 10 from 193 (out) to 208 (in) : -467.602 Transmembrane helix 11 from 212 (in) to 227 (out) : -452.015 Transmembrane helix 12 from 233 (out) to 248 (in) : -512.798 Transmembrane helix 13 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 14 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 23 (in) to 38 (out) : -596.802 Transmembrane helix 3 from 66 (out) to 81 (in) : -337.574 Transmembrane helix 4 from 85 (in) to 100 (out) : -87.472 Transmembrane helix 5 from 104 (out) to 119 (in) : -191.721 Transmembrane helix 6 from 123 (in) to 138 (out) : -623.942 Transmembrane helix 7 from 150 (out) to 165 (in) : -761.238 Transmembrane helix 8 from 174 (in) to 189 (out) : 1289.39 Transmembrane helix 9 from 193 (out) to 208 (in) : -467.602 Transmembrane helix 10 from 212 (in) to 227 (out) : -452.015 Transmembrane helix 11 from 233 (out) to 248 (in) : -512.798 Transmembrane helix 12 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 13 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 23 (out) to 38 (in) : -595.598 Transmembrane helix 3 from 65 (in) to 80 (out) : -349.538 Transmembrane helix 4 from 84 (out) to 99 (in) : -75.539 Transmembrane helix 5 from 103 (in) to 118 (out) : -214.7 Transmembrane helix 6 from 122 (out) to 137 (in) : -555.35 Transmembrane helix 7 from 174 (in) to 189 (out) : 1289.39 Transmembrane helix 8 from 193 (out) to 208 (in) : -467.602 Transmembrane helix 9 from 212 (in) to 227 (out) : -452.015 Transmembrane helix 10 from 233 (out) to 248 (in) : -512.798 Transmembrane helix 11 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 12 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 23 (in) to 38 (out) : -596.802 Transmembrane helix 3 from 68 (out) to 83 (in) : -303.082 Transmembrane helix 4 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 5 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 6 from 174 (in) to 189 (out) : 1289.39 Transmembrane helix 7 from 193 (out) to 208 (in) : -467.602 Transmembrane helix 8 from 212 (in) to 227 (out) : -452.015 Transmembrane helix 9 from 233 (out) to 248 (in) : -512.798 Transmembrane helix 10 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 11 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 23 (out) to 38 (in) : -595.598 Transmembrane helix 3 from 68 (in) to 83 (out) : -290.018 Transmembrane helix 4 from 87 (out) to 102 (in) : -76.549 Transmembrane helix 5 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 6 from 175 (out) to 190 (in) : 1311.26 Transmembrane helix 7 from 205 (in) to 220 (out) : -218.443 Transmembrane helix 8 from 233 (out) to 248 (in) : -512.798 Transmembrane helix 9 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 10 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 68 (in) to 83 (out) : -290.018 Transmembrane helix 3 from 87 (out) to 102 (in) : -76.549 Transmembrane helix 4 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 5 from 175 (out) to 190 (in) : 1311.26 Transmembrane helix 6 from 205 (in) to 220 (out) : -218.443 Transmembrane helix 7 from 233 (out) to 248 (in) : -512.798 Transmembrane helix 8 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 9 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 68 (out) to 83 (in) : -303.082 Transmembrane helix 3 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 4 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 5 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 6 from 204 (out) to 219 (in) : -208.966 Transmembrane helix 7 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 8 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 3 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 4 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 5 from 204 (out) to 219 (in) : -208.966 Transmembrane helix 6 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 7 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 85 (out) to 100 (in) : -69.428 Transmembrane helix 3 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 4 from 175 (out) to 191 (in) : 1309.38 Transmembrane helix 5 from 252 (in) to 267 (out) : -165.503 Transmembrane helix 6 from 271 (out) to 286 (in) : -182.781 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 87 (in) to 102 (out) : -82.051 Transmembrane helix 3 from 112 (out) to 127 (in) : -57.354 Transmembrane helix 4 from 175 (in) to 190 (out) : 1310.94 Transmembrane helix 5 from 263 (out) to 278 (in) : -129.02 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 85 (out) to 100 (in) : -69.428 Transmembrane helix 3 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 4 from 175 (out) to 190 (in) : 1311.26 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -14.543 Transmembrane helix 2 from 112 (in) to 127 (out) : -52.646 Transmembrane helix 3 from 175 (out) to 190 (in) : 1311.26 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -6.257 Transmembrane helix 2 from 175 (out) to 190 (in) : 1311.26 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 1.31094 1 helix (-) : Score = 1.31126 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 175-190 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 1.31126