BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (540 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P37627 Uncharacterized protein yhiJ n=45 Tax=Bacteria R...  1128   0.0  
UniRef50_P37629 Putative uncharacterized protein yhiL n=40 Tax=E...   237   8e-61

>UniRef50_P37627 Uncharacterized protein yhiJ n=45 Tax=Bacteria RepID=YHIJ_ECOLI
          Length = 540

 Score = 1128 bits (2917), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 540/540 (100%), Positives = 540/540 (100%)

Query: 1   MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLGEVVEKI 60
           MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLGEVVEKI
Sbjct: 1   MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLGEVVEKI 60

Query: 61  DDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGCS 120
           DDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGCS
Sbjct: 61  DDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGCS 120

Query: 121 LDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFA 180
           LDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFA
Sbjct: 121 LDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFA 180

Query: 181 DRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDE 240
           DRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDE
Sbjct: 181 DRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDE 240

Query: 241 VLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF 300
           VLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF
Sbjct: 241 VLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF 300

Query: 301 SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYP 360
           SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYP
Sbjct: 301 SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYP 360

Query: 361 VCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIIN 420
           VCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIIN
Sbjct: 361 VCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIIN 420

Query: 421 GKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETG 480
           GKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETG
Sbjct: 421 GKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETG 480

Query: 481 TVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL 540
           TVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL
Sbjct: 481 TVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL 540


>UniRef50_P37629 Putative uncharacterized protein yhiL n=40 Tax=Enterobacteriaceae
           RepID=YHIL_ECOLI
          Length = 535

 Score =  237 bits (605), Expect = 8e-61,   Method: Compositional matrix adjust.
 Identities = 136/396 (34%), Positives = 210/396 (53%), Gaps = 27/396 (6%)

Query: 110 VTINSKNYGCSLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDI 169
           VT+  K  G    N+  +   I+Y ++ +++        ++ ++KP W W +S+   +  
Sbjct: 126 VTVECKG-GYYFINSSGDKSDIIYKVDGLSIIARNFFTLLSGNFKPDWRWDVSKETFTKE 184

Query: 170 KFETMIKPQFADRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGEN 229
           KF++ +K  F+   ++  C  ++P +    YFGDTDG VGAVL+AL  +GH+GI  +G +
Sbjct: 185 KFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWS 244

Query: 230 FLSQLLNIEDEVLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPC 289
            L +LL  E+   +    +N           +  +LN RD+IL  L  ++ +  DA+TPC
Sbjct: 245 LLCELLKHEEMASSAYKHKN--------NKVLYDLLNTRDMILNELHQHVFLKDDAITPC 296

Query: 290 TFLGDQTGDRFSNICGDQFIIDLLKRIMS--------INENVHVLAGNHETNCNGNYMQN 341
            FLGD TGDRFS I GD++I+ LL  + +        IN+NV VLAGNHE N NGNY   
Sbjct: 297 IFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYTAR 356

Query: 342 FTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNA 401
               K    DTY+ IK   VC YD + +++ +HHGI  D+++K Y +G + V  ++M N 
Sbjct: 357 LANHKLSAGDTYNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNP 416

Query: 402 LDPVELAAIINKKHHAIINGKKFKTSRAISCRS---FNRYFSVSTDYRPKLEALLACSQM 458
            DP ELA I NKKH   ++   F   R+ + +    +  YF  +TD+RP  E +  C + 
Sbjct: 417 TDPEELANIFNKKHKEHMDDPLFHLIRSNTLKPTPVYANYFDNTTDFRPARERIFICGET 476

Query: 459 LG-------INQVVAHNGNGGRERIGETGTVLGLNA 487
           L        I Q   H+G G          ++GLN+
Sbjct: 477 LKGEDPSKYIRQKYGHHGPGVDHNQQFDNGIMGLNS 512


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P37627 Uncharacterized protein yhiJ n=45 Tax=Bacteria R...   969   0.0  
UniRef50_P37629 Putative uncharacterized protein yhiL n=40 Tax=E...   598   e-169

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P37627 Uncharacterized protein yhiJ n=45 Tax=Bacteria RepID=YHIJ_ECOLI
          Length = 540

 Score =  969 bits (2504), Expect = 0.0,   Method: Composition-based stats.
 Identities = 540/540 (100%), Positives = 540/540 (100%)

Query: 1   MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLGEVVEKI 60
           MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLGEVVEKI
Sbjct: 1   MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDDISSIARTMLGEVVEKI 60

Query: 61  DDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGCS 120
           DDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGCS
Sbjct: 61  DDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGCS 120

Query: 121 LDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFA 180
           LDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFA
Sbjct: 121 LDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQFA 180

Query: 181 DRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDE 240
           DRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDE
Sbjct: 181 DRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIEDE 240

Query: 241 VLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF 300
           VLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF
Sbjct: 241 VLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDRF 300

Query: 301 SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYP 360
           SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYP
Sbjct: 301 SNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYSGIKDYP 360

Query: 361 VCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIIN 420
           VCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIIN
Sbjct: 361 VCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAIINKKHHAIIN 420

Query: 421 GKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETG 480
           GKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETG
Sbjct: 421 GKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGGRERIGETG 480

Query: 481 TVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL 540
           TVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL
Sbjct: 481 TVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL 540


>UniRef50_P37629 Putative uncharacterized protein yhiL n=40 Tax=Enterobacteriaceae
           RepID=YHIL_ECOLI
          Length = 535

 Score =  598 bits (1542), Expect = e-169,   Method: Composition-based stats.
 Identities = 148/446 (33%), Positives = 230/446 (51%), Gaps = 41/446 (9%)

Query: 60  IDDWQIERFQQSIQDDKISFTIQTDHSEKYSMLSGMRAHILRRNNNYQFIVTINSKNYGC 119
           ID  +  RF  +  D  I FTI  D++           H+L+ ++     VT+  K  G 
Sbjct: 90  IDIQEYNRFADNTTDTFI-FTIIPDNN-----------HVLKLSS--PITVTVECKG-GY 134

Query: 120 SLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWKISQYNVSDIKFETMIKPQF 179
              N+  +   I+Y ++ +++        ++ ++KP W W +S+   +  KF++ +K  F
Sbjct: 135 YFINSSGDKSDIIYKVDGLSIIARNFFTLLSGNFKPDWRWDVSKETFTKEKFDSYVKSVF 194

Query: 180 ADRIYFSNCLPVDPTSTRPTYFGDTDGSVGAVLFALFATGHLGIMAEGENFLSQLLNIED 239
           +   ++  C  ++P +    YFGDTDG VGAVL+AL  +GH+GI  +G + L +LL  E+
Sbjct: 195 SKIDFYKQCGVINPQNANTAYFGDTDGRVGAVLYALLVSGHIGIREKGWSLLCELLKHEE 254

Query: 240 EVLNVLLRENFNEQLNTNVNTIISILNRRDIILESLQPYLVINKDAVTPCTFLGDQTGDR 299
              +    +N           +  +LN RD+IL  L  ++ +  DA+TPC FLGD TGDR
Sbjct: 255 MASSAYKHKN--------NKVLYDLLNTRDMILNELHQHVFLKDDAITPCIFLGDHTGDR 306

Query: 300 FSNICGDQFIIDLLKRIMS--------INENVHVLAGNHETNCNGNYMQNFTRMKPLDED 351
           FS I GD++I+ LL  + +        IN+NV VLAGNHE N NGNY       K    D
Sbjct: 307 FSTIFGDKYILTLLNSMRNMEGNKDSRINKNVVVLAGNHEINFNGNYTARLANHKLSAGD 366

Query: 352 TYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPITVSIDEMTNALDPVELAAII 411
           TY+ IK   VC YD + +++ +HHGI  D+++K Y +G + V  ++M N  DP ELA I 
Sbjct: 367 TYNLIKTLDVCNYDSERQVLTSHHGIIRDEEKKCYCLGALQVPFNQMKNPTDPEELANIF 426

Query: 412 NKKHHAIINGKKFKTSRAISCRS---FNRYFSVSTDYRPKLEALLACSQMLG-------I 461
           NKKH   ++   F   R+ + +    +  YF  +TD+RP  E +  C + L        I
Sbjct: 427 NKKHKEHMDDPLFHLIRSNTLKPTPVYANYFDNTTDFRPARERIFICGETLKGEDPSKYI 486

Query: 462 NQVVAHNGNGGRERIGETGTVLGLNA 487
            Q   H+G G          ++GLN+
Sbjct: 487 RQKYGHHGPGVDHNQQFDNGIMGLNS 512


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.320    0.136    0.389 

Lambda     K      H
   0.267   0.0417    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,202,128,788
Number of Sequences: 3077464
Number of extensions: 94887590
Number of successful extensions: 241143
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 241131
Number of HSP's gapped (non-prelim): 6
length of query: 540
length of database: 1,040,396,356
effective HSP length: 134
effective length of query: 406
effective length of database: 628,016,180
effective search space: 254974569080
effective search space used: 254974569080
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 96 (41.6 bits)
