Processing 1 helix: Transmembrane helix 1 from 278 (in) to 308 (out) : 4802.68 Score = 4.802676 Processing 1 helix: Transmembrane helix 1 from 280 (out) to 310 (in) : 4741.66 Score = 4.741656 Processing 20 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -105.655 Transmembrane helix 2 from 31 (out) to 46 (in) : 524.42 Transmembrane helix 3 from 50 (in) to 65 (out) : 2061.13 Transmembrane helix 4 from 69 (out) to 84 (in) : 96.955 Transmembrane helix 5 from 88 (in) to 103 (out) : 1863.12 Transmembrane helix 6 from 107 (out) to 122 (in) : 2038.13 Transmembrane helix 7 from 126 (in) to 141 (out) : -249.83 Transmembrane helix 8 from 145 (out) to 160 (in) : 1850.95 Transmembrane helix 9 from 164 (in) to 179 (out) : 1932.82 Transmembrane helix 10 from 183 (out) to 198 (in) : -7.7781 Transmembrane helix 11 from 202 (in) to 217 (out) : -290.529 Transmembrane helix 12 from 221 (out) to 236 (in) : 2452.96 Transmembrane helix 13 from 240 (in) to 255 (out) : 1178.03 Transmembrane helix 14 from 259 (out) to 274 (in) : 579.575 Transmembrane helix 15 from 278 (in) to 293 (out) : 2645.63 Transmembrane helix 16 from 297 (out) to 312 (in) : 2769.59 Transmembrane helix 17 from 316 (in) to 331 (out) : -167.742 Transmembrane helix 18 from 335 (out) to 350 (in) : 1297.78 Transmembrane helix 19 from 354 (in) to 369 (out) : 1038.57 Transmembrane helix 20 from 373 (out) to 388 (in) : 1228.49 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 77 (in) to 92 (out) : 1669.65 Transmembrane helix 5 from 96 (out) to 111 (in) : 926.956 Transmembrane helix 6 from 115 (in) to 130 (out) : 1406.3 Transmembrane helix 7 from 134 (out) to 149 (in) : 892.082 Transmembrane helix 8 from 153 (in) to 168 (out) : 465.354 Transmembrane helix 9 from 172 (out) to 187 (in) : 3272.15 Transmembrane helix 10 from 191 (in) to 206 (out) : -1488.64 Transmembrane helix 11 from 211 (out) to 226 (in) : 2483.13 Transmembrane helix 12 from 230 (in) to 245 (out) : 307.205 Transmembrane helix 13 from 249 (out) to 264 (in) : 2294.25 Transmembrane helix 14 from 268 (in) to 283 (out) : 1451.38 Transmembrane helix 15 from 287 (out) to 302 (in) : 1880.31 Transmembrane helix 16 from 306 (in) to 321 (out) : 1959.38 Transmembrane helix 17 from 326 (out) to 341 (in) : 140.476 Transmembrane helix 18 from 345 (in) to 360 (out) : 794.669 Transmembrane helix 19 from 364 (out) to 381 (in) : 3071.37 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 2300.88 Transmembrane helix 2 from 35 (out) to 50 (in) : 169.331 Transmembrane helix 3 from 54 (in) to 70 (out) : 2428.91 Transmembrane helix 4 from 77 (out) to 92 (in) : 1655.05 Transmembrane helix 5 from 96 (in) to 111 (out) : 939.344 Transmembrane helix 6 from 115 (out) to 130 (in) : 1424.5 Transmembrane helix 7 from 134 (in) to 149 (out) : 917.018 Transmembrane helix 8 from 153 (out) to 168 (in) : 463.046 Transmembrane helix 9 from 172 (in) to 187 (out) : 3238.65 Transmembrane helix 10 from 211 (out) to 226 (in) : 2483.13 Transmembrane helix 11 from 230 (in) to 245 (out) : 307.205 Transmembrane helix 12 from 249 (out) to 264 (in) : 2294.25 Transmembrane helix 13 from 268 (in) to 283 (out) : 1451.38 Transmembrane helix 14 from 287 (out) to 302 (in) : 1880.31 Transmembrane helix 15 from 306 (in) to 321 (out) : 1959.38 Transmembrane helix 16 from 326 (out) to 341 (in) : 140.476 Transmembrane helix 17 from 345 (in) to 360 (out) : 794.669 Transmembrane helix 18 from 364 (out) to 381 (in) : 3071.37 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 77 (in) to 92 (out) : 1669.65 Transmembrane helix 5 from 96 (out) to 111 (in) : 926.956 Transmembrane helix 6 from 115 (in) to 130 (out) : 1406.3 Transmembrane helix 7 from 134 (out) to 149 (in) : 892.082 Transmembrane helix 8 from 153 (in) to 168 (out) : 465.354 Transmembrane helix 9 from 172 (out) to 187 (in) : 3272.15 Transmembrane helix 10 from 213 (in) to 233 (out) : 3487.67 Transmembrane helix 11 from 237 (out) to 252 (in) : 646.803 Transmembrane helix 12 from 256 (in) to 271 (out) : 1022.43 Transmembrane helix 13 from 275 (out) to 293 (in) : 2928.82 Transmembrane helix 14 from 298 (in) to 319 (out) : 3328.62 Transmembrane helix 15 from 326 (out) to 341 (in) : 140.476 Transmembrane helix 16 from 345 (in) to 360 (out) : 794.669 Transmembrane helix 17 from 364 (out) to 381 (in) : 3071.37 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 2300.88 Transmembrane helix 2 from 35 (out) to 50 (in) : 169.331 Transmembrane helix 3 from 54 (in) to 70 (out) : 2428.91 Transmembrane helix 4 from 83 (out) to 100 (in) : 2714.5 Transmembrane helix 5 from 107 (in) to 122 (out) : 1997.27 Transmembrane helix 6 from 126 (out) to 141 (in) : -251.27 Transmembrane helix 7 from 145 (in) to 161 (out) : 1891.71 Transmembrane helix 8 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 9 from 213 (in) to 233 (out) : 3487.67 Transmembrane helix 10 from 237 (out) to 252 (in) : 646.803 Transmembrane helix 11 from 256 (in) to 271 (out) : 1022.43 Transmembrane helix 12 from 275 (out) to 293 (in) : 2928.82 Transmembrane helix 13 from 298 (in) to 319 (out) : 3328.62 Transmembrane helix 14 from 326 (out) to 341 (in) : 140.476 Transmembrane helix 15 from 345 (in) to 360 (out) : 794.669 Transmembrane helix 16 from 364 (out) to 381 (in) : 3071.37 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 5 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 6 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 7 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 8 from 213 (in) to 233 (out) : 3487.67 Transmembrane helix 9 from 237 (out) to 252 (in) : 646.803 Transmembrane helix 10 from 256 (in) to 271 (out) : 1022.43 Transmembrane helix 11 from 275 (out) to 293 (in) : 2928.82 Transmembrane helix 12 from 298 (in) to 319 (out) : 3328.62 Transmembrane helix 13 from 326 (out) to 341 (in) : 140.476 Transmembrane helix 14 from 345 (in) to 360 (out) : 794.669 Transmembrane helix 15 from 364 (out) to 381 (in) : 3071.37 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 2300.88 Transmembrane helix 2 from 35 (out) to 50 (in) : 169.331 Transmembrane helix 3 from 54 (in) to 70 (out) : 2428.91 Transmembrane helix 4 from 83 (out) to 100 (in) : 2714.5 Transmembrane helix 5 from 107 (in) to 122 (out) : 1997.27 Transmembrane helix 6 from 126 (out) to 141 (in) : -251.27 Transmembrane helix 7 from 145 (in) to 161 (out) : 1891.71 Transmembrane helix 8 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 9 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 10 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 11 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 12 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 13 from 334 (in) to 355 (out) : 1561.12 Transmembrane helix 14 from 363 (out) to 381 (in) : 3076.53 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 5 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 6 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 7 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 8 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 9 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 10 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 11 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 12 from 334 (in) to 355 (out) : 1561.12 Transmembrane helix 13 from 363 (out) to 381 (in) : 3076.53 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 4 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 5 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 6 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 7 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 8 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 9 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 10 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 11 from 334 (in) to 355 (out) : 1561.12 Transmembrane helix 12 from 363 (out) to 381 (in) : 3076.53 Score = 33.185223 Processing 11 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 51 (in) to 70 (out) : 2528.27 Transmembrane helix 3 from 85 (out) to 115 (in) : 3502.85 Transmembrane helix 4 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 5 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 6 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 7 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 8 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 9 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 10 from 334 (in) to 355 (out) : 1561.12 Transmembrane helix 11 from 363 (out) to 381 (in) : 3076.53 Score = 31.431696 Processing 10 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 4 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 5 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 6 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 7 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 8 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 9 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 10 from 348 (out) to 378 (in) : 3398.53 Re-entrant helix 1 from 317 (out) to 344 (out) : 1960 Score = 30.754223 Processing 9 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 51 (in) to 70 (out) : 2528.27 Transmembrane helix 3 from 85 (out) to 115 (in) : 3502.85 Transmembrane helix 4 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 5 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 6 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 7 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 8 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 9 from 348 (out) to 378 (in) : 3398.53 Re-entrant helix 1 from 317 (out) to 344 (out) : 1960 Score = 29.000698 Processing 8 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 4 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 5 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 6 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 7 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 8 from 348 (out) to 378 (in) : 3398.53 Re-entrant helix 1 from 317 (out) to 344 (out) : 1960 Score = 27.024464 Processing 7 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 3 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 4 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 5 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 6 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 7 from 348 (out) to 378 (in) : 3398.53 Re-entrant helix 1 from 317 (out) to 344 (out) : 1960 Score = 24.418186 Processing 6 helices: Transmembrane helix 1 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 2 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 3 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 4 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 5 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 6 from 348 (out) to 378 (in) : 3398.53 Re-entrant helix 1 from 317 (out) to 344 (out) : 1960 Score = 21.691362 Processing 5 helices: Transmembrane helix 1 from 85 (out) to 115 (in) : 3502.85 Transmembrane helix 2 from 154 (in) to 184 (out) : 3345.29 Transmembrane helix 3 from 213 (out) to 235 (in) : 3403.19 Transmembrane helix 4 from 278 (in) to 308 (out) : 4802.68 Transmembrane helix 5 from 348 (out) to 378 (in) : 3398.53 Re-entrant helix 1 from 312 (out) to 344 (out) : 2310 Score = 18.377277 Processing 4 helices: Transmembrane helix 1 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 2 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 3 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 4 from 280 (out) to 310 (in) : 4741.66 Score = 15.408633 Processing 3 helices: Transmembrane helix 1 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 2 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 3 from 280 (out) to 310 (in) : 4741.66 Score = 11.825160 Processing 2 helices: Transmembrane helix 1 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 2 from 280 (out) to 310 (in) : 4741.66 Score = 8.313605 Processing 20 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -111.845 Transmembrane helix 2 from 31 (in) to 46 (out) : 520.88 Transmembrane helix 3 from 50 (out) to 65 (in) : 2071.97 Transmembrane helix 4 from 69 (in) to 84 (out) : 70.345 Transmembrane helix 5 from 88 (out) to 103 (in) : 1812.78 Transmembrane helix 6 from 107 (in) to 122 (out) : 1997.27 Transmembrane helix 7 from 126 (out) to 141 (in) : -251.27 Transmembrane helix 8 from 145 (in) to 160 (out) : 1883.45 Transmembrane helix 9 from 164 (out) to 179 (in) : 1985.38 Transmembrane helix 10 from 183 (in) to 198 (out) : -22.2221 Transmembrane helix 11 from 202 (out) to 217 (in) : -318.871 Transmembrane helix 12 from 221 (in) to 236 (out) : 2493.44 Transmembrane helix 13 from 240 (out) to 255 (in) : 1222.17 Transmembrane helix 14 from 259 (in) to 274 (out) : 558.025 Transmembrane helix 15 from 278 (out) to 293 (in) : 2617.57 Transmembrane helix 16 from 297 (in) to 312 (out) : 2744.21 Transmembrane helix 17 from 316 (out) to 331 (in) : -153.058 Transmembrane helix 18 from 335 (in) to 350 (out) : 1307.02 Transmembrane helix 19 from 354 (out) to 369 (in) : 1044.43 Transmembrane helix 20 from 373 (in) to 388 (out) : 1214.01 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 2300.88 Transmembrane helix 2 from 35 (out) to 50 (in) : 169.331 Transmembrane helix 3 from 54 (in) to 70 (out) : 2428.91 Transmembrane helix 4 from 77 (out) to 92 (in) : 1655.05 Transmembrane helix 5 from 96 (in) to 111 (out) : 939.344 Transmembrane helix 6 from 115 (out) to 130 (in) : 1424.5 Transmembrane helix 7 from 134 (in) to 149 (out) : 917.018 Transmembrane helix 8 from 153 (out) to 168 (in) : 463.046 Transmembrane helix 9 from 172 (in) to 187 (out) : 3238.65 Transmembrane helix 10 from 191 (out) to 206 (in) : -1479.56 Transmembrane helix 11 from 213 (in) to 230 (out) : 3301.14 Transmembrane helix 12 from 234 (out) to 249 (in) : 141.646 Transmembrane helix 13 from 253 (in) to 268 (out) : 1719.74 Transmembrane helix 14 from 272 (out) to 287 (in) : 2157.3 Transmembrane helix 15 from 291 (in) to 306 (out) : 2172.06 Transmembrane helix 16 from 310 (out) to 325 (in) : 950.172 Transmembrane helix 17 from 329 (in) to 344 (out) : 622.456 Transmembrane helix 18 from 348 (out) to 363 (in) : 512.245 Transmembrane helix 19 from 367 (in) to 382 (out) : 2762.97 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 77 (in) to 92 (out) : 1669.65 Transmembrane helix 5 from 96 (out) to 111 (in) : 926.956 Transmembrane helix 6 from 115 (in) to 130 (out) : 1406.3 Transmembrane helix 7 from 134 (out) to 149 (in) : 892.082 Transmembrane helix 8 from 153 (in) to 168 (out) : 465.354 Transmembrane helix 9 from 172 (out) to 187 (in) : 3272.15 Transmembrane helix 10 from 213 (in) to 230 (out) : 3301.14 Transmembrane helix 11 from 234 (out) to 249 (in) : 141.646 Transmembrane helix 12 from 253 (in) to 268 (out) : 1719.74 Transmembrane helix 13 from 272 (out) to 287 (in) : 2157.3 Transmembrane helix 14 from 291 (in) to 306 (out) : 2172.06 Transmembrane helix 15 from 310 (out) to 325 (in) : 950.172 Transmembrane helix 16 from 329 (in) to 344 (out) : 622.456 Transmembrane helix 17 from 348 (out) to 363 (in) : 512.245 Transmembrane helix 18 from 367 (in) to 382 (out) : 2762.97 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 2300.88 Transmembrane helix 2 from 35 (out) to 50 (in) : 169.331 Transmembrane helix 3 from 54 (in) to 70 (out) : 2428.91 Transmembrane helix 4 from 83 (out) to 100 (in) : 2714.5 Transmembrane helix 5 from 107 (in) to 122 (out) : 1997.27 Transmembrane helix 6 from 126 (out) to 141 (in) : -251.27 Transmembrane helix 7 from 145 (in) to 161 (out) : 1891.71 Transmembrane helix 8 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 9 from 213 (in) to 230 (out) : 3301.14 Transmembrane helix 10 from 234 (out) to 249 (in) : 141.646 Transmembrane helix 11 from 253 (in) to 268 (out) : 1719.74 Transmembrane helix 12 from 272 (out) to 287 (in) : 2157.3 Transmembrane helix 13 from 291 (in) to 306 (out) : 2172.06 Transmembrane helix 14 from 310 (out) to 325 (in) : 950.172 Transmembrane helix 15 from 329 (in) to 344 (out) : 622.456 Transmembrane helix 16 from 348 (out) to 363 (in) : 512.245 Transmembrane helix 17 from 367 (in) to 382 (out) : 2762.97 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 5 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 6 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 7 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 8 from 213 (in) to 230 (out) : 3301.14 Transmembrane helix 9 from 234 (out) to 249 (in) : 141.646 Transmembrane helix 10 from 253 (in) to 268 (out) : 1719.74 Transmembrane helix 11 from 272 (out) to 287 (in) : 2157.3 Transmembrane helix 12 from 291 (in) to 306 (out) : 2172.06 Transmembrane helix 13 from 310 (out) to 325 (in) : 950.172 Transmembrane helix 14 from 329 (in) to 344 (out) : 622.456 Transmembrane helix 15 from 348 (out) to 363 (in) : 512.245 Transmembrane helix 16 from 367 (in) to 382 (out) : 2762.97 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 16 (in) to 31 (out) : 2300.88 Transmembrane helix 2 from 35 (out) to 50 (in) : 169.331 Transmembrane helix 3 from 54 (in) to 70 (out) : 2428.91 Transmembrane helix 4 from 83 (out) to 100 (in) : 2714.5 Transmembrane helix 5 from 107 (in) to 122 (out) : 1997.27 Transmembrane helix 6 from 126 (out) to 141 (in) : -251.27 Transmembrane helix 7 from 145 (in) to 161 (out) : 1891.71 Transmembrane helix 8 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 9 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 10 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 11 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 12 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 13 from 326 (in) to 341 (out) : 138.824 Transmembrane helix 14 from 345 (out) to 360 (in) : 776.031 Transmembrane helix 15 from 364 (in) to 381 (out) : 3010.23 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 16 (out) to 31 (in) : 2278.32 Transmembrane helix 2 from 35 (in) to 50 (out) : 178.869 Transmembrane helix 3 from 54 (out) to 71 (in) : 2449.68 Transmembrane helix 4 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 5 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 6 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 7 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 8 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 9 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 10 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 11 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 12 from 326 (in) to 341 (out) : 138.824 Transmembrane helix 13 from 345 (out) to 360 (in) : 776.031 Transmembrane helix 14 from 364 (in) to 381 (out) : 3010.23 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 4 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 5 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 6 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 7 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 8 from 246 (out) to 265 (in) : 2421.02 Transmembrane helix 9 from 274 (in) to 293 (out) : 2972.47 Transmembrane helix 10 from 298 (out) to 319 (in) : 3366.58 Transmembrane helix 11 from 326 (in) to 341 (out) : 138.824 Transmembrane helix 12 from 345 (out) to 360 (in) : 776.031 Transmembrane helix 13 from 364 (in) to 381 (out) : 3010.23 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 51 (in) to 70 (out) : 2528.27 Transmembrane helix 3 from 83 (out) to 100 (in) : 2714.5 Transmembrane helix 4 from 107 (in) to 127 (out) : 2314.89 Transmembrane helix 5 from 141 (out) to 161 (in) : 2187.8 Transmembrane helix 6 from 171 (in) to 187 (out) : 3277.47 Transmembrane helix 7 from 213 (out) to 235 (in) : 3403.19 Transmembrane helix 8 from 246 (in) to 265 (out) : 2365.18 Transmembrane helix 9 from 274 (out) to 293 (in) : 2927.93 Transmembrane helix 10 from 298 (in) to 319 (out) : 3328.62 Transmembrane helix 11 from 334 (out) to 355 (in) : 1536.88 Transmembrane helix 12 from 363 (in) to 381 (out) : 3009.67 Score = 32.045124 Processing 11 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 83 (in) to 100 (out) : 2770.3 Transmembrane helix 4 from 107 (out) to 127 (in) : 2369.31 Transmembrane helix 5 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 6 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 7 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 8 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 9 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 10 from 334 (out) to 355 (in) : 1536.88 Transmembrane helix 11 from 363 (in) to 381 (out) : 3009.67 Re-entrant helix 1 from 317 (out) to 330 (out) : 980 Score = 31.810307 Processing 10 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 51 (in) to 70 (out) : 2528.27 Transmembrane helix 3 from 85 (out) to 115 (in) : 3502.85 Transmembrane helix 4 from 141 (in) to 161 (out) : 2234.6 Transmembrane helix 5 from 171 (out) to 187 (in) : 3317.74 Transmembrane helix 6 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 7 from 249 (out) to 279 (in) : 2947.19 Transmembrane helix 8 from 283 (in) to 313 (out) : 4596.43 Transmembrane helix 9 from 334 (out) to 355 (in) : 1536.88 Transmembrane helix 10 from 363 (in) to 381 (out) : 3009.67 Re-entrant helix 1 from 317 (out) to 330 (out) : 980 Score = 30.056782 Processing 9 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 4 from 141 (out) to 161 (in) : 2187.8 Transmembrane helix 5 from 171 (in) to 187 (out) : 3277.47 Transmembrane helix 6 from 213 (out) to 235 (in) : 3403.19 Transmembrane helix 7 from 249 (in) to 279 (out) : 2896.6 Transmembrane helix 8 from 283 (out) to 313 (in) : 4572.27 Transmembrane helix 9 from 348 (in) to 378 (out) : 3345.47 Re-entrant helix 1 from 317 (in) to 344 (in) : 1960 Score = 28.326017 Processing 8 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 51 (in) to 70 (out) : 2528.27 Transmembrane helix 3 from 85 (out) to 115 (in) : 3502.85 Transmembrane helix 4 from 154 (in) to 184 (out) : 3345.29 Transmembrane helix 5 from 213 (out) to 235 (in) : 3403.19 Transmembrane helix 6 from 249 (in) to 279 (out) : 2896.6 Transmembrane helix 7 from 283 (out) to 313 (in) : 4572.27 Transmembrane helix 8 from 348 (in) to 378 (out) : 3345.47 Re-entrant helix 1 from 317 (in) to 344 (in) : 1960 Score = 26.062653 Processing 7 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 2757.92 Transmembrane helix 2 from 51 (out) to 71 (in) : 2553.12 Transmembrane helix 3 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 4 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 5 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 6 from 280 (out) to 310 (in) : 4741.66 Transmembrane helix 7 from 348 (in) to 378 (out) : 3345.47 Re-entrant helix 1 from 314 (in) to 344 (in) : 2170 Score = 24.006834 Processing 6 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 2735.28 Transmembrane helix 2 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 3 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 4 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 5 from 280 (out) to 310 (in) : 4741.66 Transmembrane helix 6 from 348 (in) to 378 (out) : 3345.47 Re-entrant helix 1 from 314 (in) to 344 (in) : 2170 Score = 21.400557 Processing 5 helices: Transmembrane helix 1 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 2 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 3 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 4 from 280 (out) to 310 (in) : 4741.66 Transmembrane helix 5 from 348 (in) to 378 (out) : 3345.47 Re-entrant helix 1 from 314 (in) to 344 (in) : 2170 Score = 18.673735 Processing 4 helices: Transmembrane helix 1 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 2 from 213 (in) to 234 (out) : 3494.87 Transmembrane helix 3 from 280 (out) to 310 (in) : 4741.66 Transmembrane helix 4 from 348 (in) to 378 (out) : 3345.47 Re-entrant helix 1 from 314 (in) to 344 (in) : 2170 Score = 15.090261 Processing 3 helices: Transmembrane helix 1 from 84 (in) to 114 (out) : 3571.95 Transmembrane helix 2 from 154 (out) to 184 (in) : 3432.71 Transmembrane helix 3 from 278 (in) to 308 (out) : 4802.68 Score = 11.818858 Processing 2 helices: Transmembrane helix 1 from 85 (out) to 115 (in) : 3502.85 Transmembrane helix 2 from 278 (in) to 308 (out) : 4802.68 Score = 8.305529 Summary of topology analysis: 1 helix (+) : Score = 4.80268 1 helix (-) : Score = 4.74166 2 helices (+) : Score = 8.31361 2 helices (-) : Score = 8.30553 3 helices (+) : Score = 11.8189 3 helices (-) : Score = 11.8252 4 helices (+) : Score = 15.4086 4 helices (-) : Score = 15.0903 5 helices (+) : Score = 18.6737 5 helices (-) : Score = 18.3773 6 helices (+) : Score = 21.6914 6 helices (-) : Score = 21.4006 7 helices (+) : Score = 24.0068 7 helices (-) : Score = 24.4182 8 helices (+) : Score = 27.0245 8 helices (-) : Score = 26.0627 9 helices (+) : Score = 28.326 9 helices (-) : Score = 29.0007 10 helices (+) : Score = 30.7542 10 helices (-) : Score = 30.0568 11 helices (+) : Score = 31.8103 11 helices (-) : Score = 31.4317 12 helices (+) : Score = 33.1852 12 helices (-) : Score = 32.0451 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 Results: Signal peptide: 1-13 Signal score: 16.01 Topology: 16-39,51-71,83-100,107-127,141-161,171-187,213-234,246-265,274-293,298-319,334-355,363-381 Re-entrant helices: Not detected. Helix count: 12 N-terminal: out Score: 33.1852