Processing 1 helix: Transmembrane helix 1 from 247 (in) to 277 (out) : 5470.65 Score = 5.470650 Processing 1 helix: Transmembrane helix 1 from 247 (out) to 277 (in) : 5447.25 Score = 5.447250 Processing 23 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -729.491 Transmembrane helix 2 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 3 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 4 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 5 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 6 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 7 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 8 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 9 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 10 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 11 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 12 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 13 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 14 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 15 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 16 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 17 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 18 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 19 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 20 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 21 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 22 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 23 from 1 (in) to 0 (out) : 1.542 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 6 (out) to 21 (in) : -250.039 Transmembrane helix 2 from 25 (in) to 40 (out) : 2376.22 Transmembrane helix 3 from 44 (out) to 59 (in) : 40.476 Transmembrane helix 4 from 63 (in) to 78 (out) : 2078.45 Transmembrane helix 5 from 82 (out) to 97 (in) : -303.762 Transmembrane helix 6 from 101 (in) to 116 (out) : 2552.57 Transmembrane helix 7 from 120 (out) to 135 (in) : 860.421 Transmembrane helix 8 from 139 (in) to 154 (out) : 1692.45 Transmembrane helix 9 from 158 (out) to 173 (in) : -218.257 Transmembrane helix 10 from 177 (in) to 192 (out) : 1544.58 Transmembrane helix 11 from 196 (out) to 211 (in) : 1400.44 Transmembrane helix 12 from 226 (in) to 241 (out) : -1152.36 Transmembrane helix 13 from 245 (out) to 262 (in) : 4675.75 Transmembrane helix 14 from 267 (in) to 282 (out) : 1868.13 Transmembrane helix 15 from 286 (out) to 301 (in) : 242.101 Transmembrane helix 16 from 305 (in) to 320 (out) : 1407.72 Transmembrane helix 17 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 18 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 19 from 362 (out) to 377 (in) : 98.377 Transmembrane helix 20 from 381 (in) to 396 (out) : 355.163 Transmembrane helix 21 from 400 (out) to 415 (in) : 1103.27 Transmembrane helix 22 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 19 (in) to 36 (out) : 2738.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -313.266 Transmembrane helix 3 from 59 (in) to 74 (out) : 2397.8 Transmembrane helix 4 from 78 (out) to 93 (in) : -268.554 Transmembrane helix 5 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 6 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 7 from 135 (in) to 150 (out) : 1656.59 Transmembrane helix 8 from 154 (out) to 169 (in) : -6.91998 Transmembrane helix 9 from 173 (in) to 188 (out) : 553.979 Transmembrane helix 10 from 192 (out) to 207 (in) : 2729.92 Transmembrane helix 11 from 226 (in) to 241 (out) : -1152.36 Transmembrane helix 12 from 245 (out) to 262 (in) : 4675.75 Transmembrane helix 13 from 267 (in) to 282 (out) : 1868.13 Transmembrane helix 14 from 286 (out) to 301 (in) : 242.101 Transmembrane helix 15 from 305 (in) to 320 (out) : 1407.72 Transmembrane helix 16 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 17 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 18 from 362 (out) to 377 (in) : 98.377 Transmembrane helix 19 from 381 (in) to 396 (out) : 355.163 Transmembrane helix 20 from 400 (out) to 415 (in) : 1103.27 Transmembrane helix 21 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 19 (out) to 36 (in) : 2711.15 Transmembrane helix 2 from 40 (in) to 55 (out) : -315.934 Transmembrane helix 3 from 59 (out) to 74 (in) : 2412 Transmembrane helix 4 from 78 (in) to 93 (out) : -275.646 Transmembrane helix 5 from 97 (out) to 112 (in) : 2280.54 Transmembrane helix 6 from 116 (in) to 131 (out) : 1392.56 Transmembrane helix 7 from 135 (out) to 150 (in) : 1637.61 Transmembrane helix 8 from 154 (in) to 169 (out) : -3.17998 Transmembrane helix 9 from 173 (out) to 188 (in) : 578.121 Transmembrane helix 10 from 192 (in) to 207 (out) : 2723.08 Transmembrane helix 11 from 244 (out) to 262 (in) : 4682.97 Transmembrane helix 12 from 267 (in) to 282 (out) : 1868.13 Transmembrane helix 13 from 286 (out) to 301 (in) : 242.101 Transmembrane helix 14 from 305 (in) to 320 (out) : 1407.72 Transmembrane helix 15 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 16 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 17 from 362 (out) to 377 (in) : 98.377 Transmembrane helix 18 from 381 (in) to 396 (out) : 355.163 Transmembrane helix 19 from 400 (out) to 415 (in) : 1103.27 Transmembrane helix 20 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 19 (in) to 36 (out) : 2738.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -313.266 Transmembrane helix 3 from 59 (in) to 74 (out) : 2397.8 Transmembrane helix 4 from 78 (out) to 93 (in) : -268.554 Transmembrane helix 5 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 6 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 7 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 8 from 161 (out) to 176 (in) : -250.392 Transmembrane helix 9 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 10 from 244 (out) to 262 (in) : 4682.97 Transmembrane helix 11 from 267 (in) to 282 (out) : 1868.13 Transmembrane helix 12 from 286 (out) to 301 (in) : 242.101 Transmembrane helix 13 from 305 (in) to 320 (out) : 1407.72 Transmembrane helix 14 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 15 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 16 from 362 (out) to 377 (in) : 98.377 Transmembrane helix 17 from 381 (in) to 396 (out) : 355.163 Transmembrane helix 18 from 400 (out) to 415 (in) : 1103.27 Transmembrane helix 19 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 52 (in) to 67 (out) : 1557.94 Transmembrane helix 3 from 71 (out) to 86 (in) : 725.815 Transmembrane helix 4 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 5 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 6 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 7 from 161 (out) to 176 (in) : -250.392 Transmembrane helix 8 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 9 from 244 (out) to 262 (in) : 4682.97 Transmembrane helix 10 from 267 (in) to 282 (out) : 1868.13 Transmembrane helix 11 from 286 (out) to 301 (in) : 242.101 Transmembrane helix 12 from 305 (in) to 320 (out) : 1407.72 Transmembrane helix 13 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 14 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 15 from 362 (out) to 377 (in) : 98.377 Transmembrane helix 16 from 381 (in) to 396 (out) : 355.163 Transmembrane helix 17 from 400 (out) to 415 (in) : 1103.27 Transmembrane helix 18 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 70 (in) : 2068.25 Transmembrane helix 3 from 74 (in) to 89 (out) : 212.532 Transmembrane helix 4 from 97 (out) to 112 (in) : 2280.54 Transmembrane helix 5 from 116 (in) to 131 (out) : 1392.56 Transmembrane helix 6 from 135 (out) to 157 (in) : 2008.39 Transmembrane helix 7 from 161 (in) to 176 (out) : -259.808 Transmembrane helix 8 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 9 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 10 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 11 from 298 (in) to 319 (out) : 1613.65 Transmembrane helix 12 from 323 (out) to 338 (in) : 423.769 Transmembrane helix 13 from 342 (in) to 357 (out) : 1606.08 Transmembrane helix 14 from 361 (out) to 376 (in) : 105.897 Transmembrane helix 15 from 380 (in) to 395 (out) : 277.437 Transmembrane helix 16 from 399 (out) to 415 (in) : 1192.53 Transmembrane helix 17 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 52 (in) to 67 (out) : 1557.94 Transmembrane helix 3 from 71 (out) to 86 (in) : 725.815 Transmembrane helix 4 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 5 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 6 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 7 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 8 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 9 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 10 from 298 (in) to 319 (out) : 1613.65 Transmembrane helix 11 from 323 (out) to 338 (in) : 423.769 Transmembrane helix 12 from 342 (in) to 357 (out) : 1606.08 Transmembrane helix 13 from 361 (out) to 376 (in) : 105.897 Transmembrane helix 14 from 380 (in) to 395 (out) : 277.437 Transmembrane helix 15 from 399 (out) to 415 (in) : 1192.53 Transmembrane helix 16 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 4 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 5 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 6 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 7 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 8 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 9 from 298 (in) to 319 (out) : 1613.65 Transmembrane helix 10 from 323 (out) to 338 (in) : 423.769 Transmembrane helix 11 from 342 (in) to 357 (out) : 1606.08 Transmembrane helix 12 from 361 (out) to 376 (in) : 105.897 Transmembrane helix 13 from 380 (in) to 395 (out) : 277.437 Transmembrane helix 14 from 399 (out) to 415 (in) : 1192.53 Transmembrane helix 15 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 7 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 8 from 298 (in) to 319 (out) : 1613.65 Transmembrane helix 9 from 323 (out) to 338 (in) : 423.769 Transmembrane helix 10 from 342 (in) to 357 (out) : 1606.08 Transmembrane helix 11 from 361 (out) to 376 (in) : 105.897 Transmembrane helix 12 from 380 (in) to 395 (out) : 277.437 Transmembrane helix 13 from 399 (out) to 415 (in) : 1192.53 Transmembrane helix 14 from 419 (in) to 437 (out) : 3194.15 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 4 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 5 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 6 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 7 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 8 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 9 from 298 (in) to 323 (out) : 1719.22 Transmembrane helix 10 from 327 (out) to 357 (in) : 1993.58 Transmembrane helix 11 from 361 (in) to 376 (out) : 105.003 Transmembrane helix 12 from 385 (out) to 412 (in) : 1664.73 Transmembrane helix 13 from 416 (in) to 437 (out) : 3221.87 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 7 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 8 from 298 (in) to 323 (out) : 1719.22 Transmembrane helix 9 from 327 (out) to 357 (in) : 1993.58 Transmembrane helix 10 from 361 (in) to 376 (out) : 105.003 Transmembrane helix 11 from 385 (out) to 412 (in) : 1664.73 Transmembrane helix 12 from 416 (in) to 437 (out) : 3221.87 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 135 (out) to 157 (in) : 2008.39 Transmembrane helix 5 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 6 from 244 (out) to 262 (in) : 4682.97 Transmembrane helix 7 from 271 (in) to 287 (out) : 2264.51 Transmembrane helix 8 from 298 (out) to 323 (in) : 1684.18 Transmembrane helix 9 from 327 (in) to 357 (out) : 1995.92 Transmembrane helix 10 from 385 (out) to 412 (in) : 1664.73 Transmembrane helix 11 from 416 (in) to 437 (out) : 3221.87 Score = 30.994080 Processing 10 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 7 from 271 (out) to 300 (in) : 2360.75 Transmembrane helix 8 from 304 (in) to 334 (out) : 1887.08 Transmembrane helix 9 from 341 (out) to 371 (in) : 1760.66 Transmembrane helix 10 from 407 (in) to 437 (out) : 3674.29 Score = 29.757812 Processing 9 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 135 (out) to 157 (in) : 2008.39 Transmembrane helix 5 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 6 from 246 (out) to 276 (in) : 5421.6 Transmembrane helix 7 from 280 (in) to 310 (out) : 1856.67 Transmembrane helix 8 from 327 (out) to 357 (in) : 1993.58 Transmembrane helix 9 from 407 (in) to 437 (out) : 3674.29 Score = 28.358587 Processing 8 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 247 (in) to 277 (out) : 5470.65 Transmembrane helix 7 from 327 (out) to 357 (in) : 1993.58 Transmembrane helix 8 from 407 (in) to 437 (out) : 3674.29 Score = 26.464457 Processing 7 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 5 from 247 (in) to 277 (out) : 5470.65 Transmembrane helix 6 from 327 (out) to 357 (in) : 1993.58 Transmembrane helix 7 from 407 (in) to 437 (out) : 3674.29 Score = 24.741955 Processing 6 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 5 from 247 (out) to 277 (in) : 5447.25 Transmembrane helix 6 from 407 (in) to 437 (out) : 3674.29 Score = 22.259502 Processing 5 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 3 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 4 from 247 (out) to 277 (in) : 5447.25 Transmembrane helix 5 from 407 (in) to 437 (out) : 3674.29 Score = 19.759365 Processing 4 helices: Transmembrane helix 1 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 2 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 3 from 247 (out) to 277 (in) : 5447.25 Transmembrane helix 4 from 407 (in) to 437 (out) : 3674.29 Score = 16.852751 Processing 3 helices: Transmembrane helix 1 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 2 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 3 from 247 (in) to 277 (out) : 5470.65 Score = 13.480462 Processing 2 helices: Transmembrane helix 1 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 2 from 247 (in) to 277 (out) : 5470.65 Score = 9.411643 Processing 23 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -740.915 Transmembrane helix 2 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 3 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 4 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 5 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 6 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 7 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 8 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 9 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 10 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 11 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 12 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 13 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 14 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 15 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 16 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 17 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 18 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 19 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 20 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 21 from 1 (out) to 0 (in) : -4.262 Transmembrane helix 22 from 1 (in) to 0 (out) : 1.542 Transmembrane helix 23 from 1 (out) to 0 (in) : -4.262 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 6 (in) to 21 (out) : -247.061 Transmembrane helix 2 from 25 (out) to 40 (in) : 2360.18 Transmembrane helix 3 from 44 (in) to 59 (out) : 30.424 Transmembrane helix 4 from 63 (out) to 78 (in) : 2097.15 Transmembrane helix 5 from 82 (in) to 97 (out) : -301.638 Transmembrane helix 6 from 101 (out) to 116 (in) : 2543.43 Transmembrane helix 7 from 120 (in) to 135 (out) : 859.079 Transmembrane helix 8 from 139 (out) to 154 (in) : 1682.55 Transmembrane helix 9 from 158 (in) to 173 (out) : -223.743 Transmembrane helix 10 from 177 (out) to 192 (in) : 1573.62 Transmembrane helix 11 from 196 (in) to 211 (out) : 1394.96 Transmembrane helix 12 from 226 (out) to 241 (in) : -1141.65 Transmembrane helix 13 from 245 (in) to 262 (out) : 4713.45 Transmembrane helix 14 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 15 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 16 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 17 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 18 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 19 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 20 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 21 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 22 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 19 (out) to 36 (in) : 2711.15 Transmembrane helix 2 from 40 (in) to 55 (out) : -315.934 Transmembrane helix 3 from 59 (out) to 74 (in) : 2412 Transmembrane helix 4 from 78 (in) to 93 (out) : -275.646 Transmembrane helix 5 from 97 (out) to 112 (in) : 2280.54 Transmembrane helix 6 from 116 (in) to 131 (out) : 1392.56 Transmembrane helix 7 from 135 (out) to 150 (in) : 1637.61 Transmembrane helix 8 from 154 (in) to 169 (out) : -3.17998 Transmembrane helix 9 from 173 (out) to 188 (in) : 578.121 Transmembrane helix 10 from 192 (in) to 207 (out) : 2723.08 Transmembrane helix 11 from 226 (out) to 241 (in) : -1141.65 Transmembrane helix 12 from 245 (in) to 262 (out) : 4713.45 Transmembrane helix 13 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 14 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 15 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 16 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 17 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 18 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 19 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 20 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 21 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 19 (in) to 36 (out) : 2738.25 Transmembrane helix 2 from 40 (out) to 55 (in) : -313.266 Transmembrane helix 3 from 59 (in) to 74 (out) : 2397.8 Transmembrane helix 4 from 78 (out) to 93 (in) : -268.554 Transmembrane helix 5 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 6 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 7 from 135 (in) to 150 (out) : 1656.59 Transmembrane helix 8 from 154 (out) to 169 (in) : -6.91998 Transmembrane helix 9 from 173 (in) to 188 (out) : 553.979 Transmembrane helix 10 from 192 (out) to 207 (in) : 2729.92 Transmembrane helix 11 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 12 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 13 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 14 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 15 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 16 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 17 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 18 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 19 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 20 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 19 (out) to 37 (in) : 2788.77 Transmembrane helix 2 from 41 (in) to 56 (out) : -266.322 Transmembrane helix 3 from 60 (out) to 75 (in) : 2407.1 Transmembrane helix 4 from 79 (in) to 94 (out) : -334.136 Transmembrane helix 5 from 98 (out) to 113 (in) : 2400.36 Transmembrane helix 6 from 117 (in) to 132 (out) : 1240.51 Transmembrane helix 7 from 136 (out) to 157 (in) : 1983.81 Transmembrane helix 8 from 161 (in) to 176 (out) : -259.808 Transmembrane helix 9 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 10 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 11 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 12 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 13 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 14 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 15 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 16 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 17 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 18 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 19 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 70 (in) : 2068.25 Transmembrane helix 3 from 74 (in) to 89 (out) : 212.532 Transmembrane helix 4 from 97 (out) to 112 (in) : 2280.54 Transmembrane helix 5 from 116 (in) to 131 (out) : 1392.56 Transmembrane helix 6 from 135 (out) to 157 (in) : 2008.39 Transmembrane helix 7 from 161 (in) to 176 (out) : -259.808 Transmembrane helix 8 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 9 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 10 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 11 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 12 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 13 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 14 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 15 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 16 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 17 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 18 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 52 (in) to 67 (out) : 1557.94 Transmembrane helix 3 from 71 (out) to 86 (in) : 725.815 Transmembrane helix 4 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 5 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 6 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 7 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 8 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 9 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 10 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 11 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 12 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 13 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 14 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 15 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 16 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 17 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 4 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 5 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 6 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 7 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 8 from 267 (out) to 282 (in) : 1880.27 Transmembrane helix 9 from 286 (in) to 301 (out) : 251.199 Transmembrane helix 10 from 305 (out) to 320 (in) : 1390.88 Transmembrane helix 11 from 324 (in) to 339 (out) : 404.224 Transmembrane helix 12 from 343 (out) to 358 (in) : 1575.68 Transmembrane helix 13 from 362 (in) to 377 (out) : 98.023 Transmembrane helix 14 from 381 (out) to 396 (in) : 370.637 Transmembrane helix 15 from 400 (in) to 415 (out) : 1090.43 Transmembrane helix 16 from 419 (out) to 437 (in) : 3168.25 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 52 (in) to 67 (out) : 1557.94 Transmembrane helix 3 from 71 (out) to 86 (in) : 725.815 Transmembrane helix 4 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 5 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 6 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 7 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 8 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 9 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 10 from 298 (in) to 320 (out) : 1670.32 Transmembrane helix 11 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 12 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 13 from 364 (out) to 379 (in) : 105.209 Transmembrane helix 14 from 385 (in) to 412 (out) : 1654.07 Transmembrane helix 15 from 416 (out) to 437 (in) : 3184.03 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 97 (in) to 112 (out) : 2304.66 Transmembrane helix 4 from 116 (out) to 131 (in) : 1392.05 Transmembrane helix 5 from 135 (in) to 157 (out) : 2031.21 Transmembrane helix 6 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 7 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 8 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 9 from 298 (in) to 320 (out) : 1670.32 Transmembrane helix 10 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 11 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 12 from 364 (out) to 379 (in) : 105.209 Transmembrane helix 13 from 385 (in) to 412 (out) : 1654.07 Transmembrane helix 14 from 416 (out) to 437 (in) : 3184.03 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 7 from 271 (out) to 287 (in) : 2294.09 Transmembrane helix 8 from 298 (in) to 320 (out) : 1670.32 Transmembrane helix 9 from 324 (out) to 339 (in) : 411.276 Transmembrane helix 10 from 343 (in) to 358 (out) : 1565.32 Transmembrane helix 11 from 364 (out) to 379 (in) : 105.209 Transmembrane helix 12 from 385 (in) to 412 (out) : 1654.07 Transmembrane helix 13 from 416 (out) to 437 (in) : 3184.03 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 135 (out) to 157 (in) : 2008.39 Transmembrane helix 5 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 6 from 244 (out) to 262 (in) : 4682.97 Transmembrane helix 7 from 271 (in) to 287 (out) : 2264.51 Transmembrane helix 8 from 298 (out) to 323 (in) : 1684.18 Transmembrane helix 9 from 327 (in) to 357 (out) : 1995.92 Transmembrane helix 10 from 364 (out) to 379 (in) : 105.209 Transmembrane helix 11 from 385 (in) to 412 (out) : 1654.07 Transmembrane helix 12 from 416 (out) to 437 (in) : 3184.03 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 244 (in) to 262 (out) : 4723.63 Transmembrane helix 7 from 271 (out) to 300 (in) : 2360.75 Transmembrane helix 8 from 304 (in) to 334 (out) : 1887.08 Transmembrane helix 9 from 341 (out) to 371 (in) : 1760.66 Transmembrane helix 10 from 385 (in) to 412 (out) : 1654.07 Transmembrane helix 11 from 416 (out) to 437 (in) : 3184.03 Score = 30.932697 Processing 10 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 135 (out) to 157 (in) : 2008.39 Transmembrane helix 5 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 6 from 244 (out) to 262 (in) : 4682.97 Transmembrane helix 7 from 271 (in) to 287 (out) : 2264.51 Transmembrane helix 8 from 298 (out) to 323 (in) : 1684.18 Transmembrane helix 9 from 327 (in) to 357 (out) : 1995.92 Transmembrane helix 10 from 407 (out) to 437 (in) : 3647.91 Score = 29.766464 Processing 9 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 246 (in) to 276 (out) : 5450.1 Transmembrane helix 7 from 280 (out) to 310 (in) : 1845.23 Transmembrane helix 8 from 326 (in) to 356 (out) : 1992.25 Transmembrane helix 9 from 407 (out) to 437 (in) : 3647.91 Score = 28.351093 Processing 8 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 5 from 246 (in) to 276 (out) : 5450.1 Transmembrane helix 6 from 280 (out) to 310 (in) : 1845.23 Transmembrane helix 7 from 326 (in) to 356 (out) : 1992.25 Transmembrane helix 8 from 407 (out) to 437 (in) : 3647.91 Score = 26.628592 Processing 7 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 55 (in) to 81 (out) : 2668.15 Transmembrane helix 3 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 4 from 134 (in) to 157 (out) : 2030.69 Transmembrane helix 5 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 6 from 247 (in) to 277 (out) : 5470.65 Transmembrane helix 7 from 407 (out) to 437 (in) : 3647.91 Score = 24.486498 Processing 6 helices: Transmembrane helix 1 from 19 (in) to 38 (out) : 2854.31 Transmembrane helix 2 from 55 (out) to 81 (in) : 2702.65 Transmembrane helix 3 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 4 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 5 from 247 (in) to 277 (out) : 5470.65 Transmembrane helix 6 from 407 (out) to 437 (in) : 3647.91 Score = 22.763998 Processing 5 helices: Transmembrane helix 1 from 19 (out) to 38 (in) : 2823.09 Transmembrane helix 2 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 3 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 4 from 247 (in) to 277 (out) : 5470.65 Transmembrane helix 5 from 407 (out) to 437 (in) : 3647.91 Score = 19.893335 Processing 4 helices: Transmembrane helix 1 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 2 from 183 (out) to 205 (in) : 3929.05 Transmembrane helix 3 from 247 (in) to 277 (out) : 5470.65 Transmembrane helix 4 from 407 (out) to 437 (in) : 3647.91 Score = 17.122551 Processing 3 helices: Transmembrane helix 1 from 98 (out) to 128 (in) : 3940.99 Transmembrane helix 2 from 183 (in) to 205 (out) : 3908.55 Transmembrane helix 3 from 247 (out) to 277 (in) : 5447.25 Score = 13.172639 Processing 2 helices: Transmembrane helix 1 from 98 (in) to 128 (out) : 3956.61 Transmembrane helix 2 from 247 (out) to 277 (in) : 5447.25 Score = 9.403857 Summary of topology analysis: 1 helix (+) : Score = 5.47065 1 helix (-) : Score = 5.44725 2 helices (+) : Score = 9.40386 2 helices (-) : Score = 9.41164 3 helices (+) : Score = 13.4805 3 helices (-) : Score = 13.1726 4 helices (+) : Score = 17.1226 4 helices (-) : Score = 16.8528 5 helices (+) : Score = 19.7594 5 helices (-) : Score = 19.8933 6 helices (+) : Score = 22.764 6 helices (-) : Score = 22.2595 7 helices (+) : Score = 24.742 7 helices (-) : Score = 24.4865 8 helices (+) : Score = 26.6286 8 helices (-) : Score = 26.4645 9 helices (+) : Score = 28.3586 9 helices (-) : Score = 28.3511 10 helices (+) : Score = 29.7665 10 helices (-) : Score = 29.7578 11 helices (+) : Score = 30.9941 11 helices (-) : Score = 30.9327 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 3.057 Topology: 19-38,55-81,98-128,135-157,183-205,244-262,271-287,298-323,327-357,385-412,416-437 Re-entrant helices: Not detected. Helix count: 11 N-terminal: in Score: 30.9941