BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (289 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P39279 Uncharacterized protein yjeJ n=102 Tax=Enterobac...   598   e-170
UniRef50_B5Y366 Putative uncharacterized protein n=5 Tax=Klebsie...   126   1e-27

>UniRef50_P39279 Uncharacterized protein yjeJ n=102 Tax=Enterobacteriaceae
           RepID=YJEJ_ECOLI
          Length = 289

 Score =  598 bits (1542), Expect = e-170,   Method: Compositional matrix adjust.
 Identities = 289/289 (100%), Positives = 289/289 (100%)

Query: 1   MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL 60
           MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL
Sbjct: 1   MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL 60

Query: 61  DAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLH 120
           DAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLH
Sbjct: 61  DAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLH 120

Query: 121 DGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYD 180
           DGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYD
Sbjct: 121 DGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYD 180

Query: 181 TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDFSPR 240
           TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDFSPR
Sbjct: 181 TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDFSPR 240

Query: 241 LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK 289
           LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK
Sbjct: 241 LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK 289


>UniRef50_B5Y366 Putative uncharacterized protein n=5 Tax=Klebsiella
           RepID=B5Y366_KLEP3
          Length = 287

 Score =  126 bits (316), Expect = 1e-27,   Method: Compositional matrix adjust.
 Identities = 88/279 (31%), Positives = 145/279 (51%), Gaps = 9/279 (3%)

Query: 1   MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL 60
           M  +  G NT  I   + F+AL LKIK+P      +++    L DLL+ L++RL   +K 
Sbjct: 1   MQGTFIGFNTAGITFEDRFLALLLKIKQPNGLCQQYYLQAPILLDLLLILQNRLLVTYK- 59

Query: 61  DAAAHLQYEQARDKVI---KKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVL 117
               H Q E  ++++I   + +  NIP + + E++  +  RR+ ++ L   + E+ T +L
Sbjct: 60  --RLHEQGETYKEELIAYNESLIANIPAVEMAEIQQPNPERRIMSITLKPGETES-TLIL 116

Query: 118 TLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNL 177
            L +   C L + + Q+E L   I  A+     + +   ++S LDFL  Y VD     N+
Sbjct: 117 VLQNEQICTLCIEDRQVEALLTGIQQALKTVNDQAVIQYLSSNLDFLMCYAVDLTTQPNI 176

Query: 178 EYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDF 237
           +Y  Y Q EWK NLF HYL  LY  + + GK+  SGAVVKT      +    +  ++++ 
Sbjct: 177 DYQQYPQEEWKLNLFSHYLGALYCCETDEGKKIVSGAVVKTSVPHLSELENNVVMQLIEK 236

Query: 238 SPRLKKLAG--VPCQVYVRTVAANNAQPLTQDQCLRALH 274
           SP+LK +     PCQ++   + +   + L  ++CLR LH
Sbjct: 237 SPKLKAMHAELAPCQIFSTIIPSQPGRMLILEECLRPLH 275


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P39279 Uncharacterized protein yjeJ n=102 Tax=Enterobac...   461   e-128
UniRef50_B5Y366 Putative uncharacterized protein n=5 Tax=Klebsie...   384   e-105

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P39279 Uncharacterized protein yjeJ n=102 Tax=Enterobacteriaceae
           RepID=YJEJ_ECOLI
          Length = 289

 Score =  461 bits (1185), Expect = e-128,   Method: Composition-based stats.
 Identities = 289/289 (100%), Positives = 289/289 (100%)

Query: 1   MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL 60
           MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL
Sbjct: 1   MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL 60

Query: 61  DAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLH 120
           DAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLH
Sbjct: 61  DAAAHLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLH 120

Query: 121 DGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYD 180
           DGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYD
Sbjct: 121 DGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYD 180

Query: 181 TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDFSPR 240
           TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDFSPR
Sbjct: 181 TYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDFSPR 240

Query: 241 LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK 289
           LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK
Sbjct: 241 LKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK 289


>UniRef50_B5Y366 Putative uncharacterized protein n=5 Tax=Klebsiella
           RepID=B5Y366_KLEP3
          Length = 287

 Score =  384 bits (985), Expect = e-105,   Method: Composition-based stats.
 Identities = 88/288 (30%), Positives = 149/288 (51%), Gaps = 9/288 (3%)

Query: 1   MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELRDLLIALESRLHQKHKL 60
           M  +  G NT  I   + F+AL LKIK+P      +++    L DLL+ L++RL   +K 
Sbjct: 1   MQGTFIGFNTAGITFEDRFLALLLKIKQPNGLCQQYYLQAPILLDLLLILQNRLLVTYK- 59

Query: 61  DAAAHLQYEQARDKVI---KKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVL 117
               H Q E  ++++I   + +  NIP + + E++  +  RR+ ++ L   + E+ T +L
Sbjct: 60  --RLHEQGETYKEELIAYNESLIANIPAVEMAEIQQPNPERRIMSITLKPGETES-TLIL 116

Query: 118 TLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNL 177
            L +   C L + + Q+E L   I  A+     + +   ++S LDFL  Y VD     N+
Sbjct: 117 VLQNEQICTLCIEDRQVEALLTGIQQALKTVNDQAVIQYLSSNLDFLMCYAVDLTTQPNI 176

Query: 178 EYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKTREATPGKEIEAITRRMLDF 237
           +Y  Y Q EWK NLF HYL  LY  + + GK+  SGAVVKT      +    +  ++++ 
Sbjct: 177 DYQQYPQEEWKLNLFSHYLGALYCCETDEGKKIVSGAVVKTSVPHLSELENNVVMQLIEK 236

Query: 238 SPRLKKLAG--VPCQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSK 283
           SP+LK +     PCQ++   + +   + L  ++CLR LH   ++  ++
Sbjct: 237 SPKLKAMHAELAPCQIFSTIIPSQPGRMLILEECLRPLHAFYLEKKAE 284


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.315    0.133    0.348 

Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 982,772,623
Number of Sequences: 3077464
Number of extensions: 36423009
Number of successful extensions: 106153
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 106143
Number of HSP's gapped (non-prelim): 4
length of query: 289
length of database: 1,040,396,356
effective HSP length: 127
effective length of query: 162
effective length of database: 649,558,428
effective search space: 105228465336
effective search space used: 105228465336
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.5 bits)
S2: 92 (40.0 bits)
