BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (69 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P33230 Uncharacterized protein ydaC n=26 Tax=Enterobact...   140   2e-32
UniRef50_B7MZY6 YheB protein n=1 Tax=Escherichia coli ED1a RepID...    63   3e-09
UniRef50_Q9F505 YheB protein n=2 Tax=Escherichia coli RepID=Q9F5...    54   2e-06

>UniRef50_P33230 Uncharacterized protein ydaC n=26 Tax=Enterobacteriaceae
          RepID=YDAC_ECOLI
          Length = 69

 Score =  140 bits (352), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 69/69 (100%), Positives = 69/69 (100%)

Query: 1  MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQ 60
          MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQ
Sbjct: 1  MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQ 60

Query: 61 SSRIALYSN 69
          SSRIALYSN
Sbjct: 61 SSRIALYSN 69


>UniRef50_B7MZY6 YheB protein n=1 Tax=Escherichia coli ED1a RepID=B7MZY6_ECO81
          Length = 76

 Score = 63.2 bits (152), Expect = 3e-09,   Method: Compositional matrix adjust.
 Identities = 29/55 (52%), Positives = 37/55 (67%)

Query: 2  YKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNC 56
          Y I ATIEK GGTP +W R+  + +TK ECEK+ S +KEAG S   KV +  F+C
Sbjct: 13 YCIQATIEKPGGTPVSWIRFQNTPMTKQECEKLFSTEKEAGKSFTVKVGVTQFSC 67


>UniRef50_Q9F505 YheB protein n=2 Tax=Escherichia coli RepID=Q9F505_ECOLX
          Length = 71

 Score = 53.5 bits (127), Expect = 2e-06,   Method: Compositional matrix adjust.
 Identities = 25/60 (41%), Positives = 34/60 (56%)

Query: 2  YKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQS 61
          Y+I ATI+K GG P  WT YS   +T  EC K  S +KEAG      V + +F C+++  
Sbjct: 4  YQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAGKCFSVDVHISDFICKEIPP 63


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q9F505 YheB protein n=2 Tax=Escherichia coli RepID=Q9F5...   108   4e-23
UniRef50_P33230 Uncharacterized protein ydaC n=26 Tax=Enterobact...   104   7e-22
UniRef50_B7MZY6 YheB protein n=1 Tax=Escherichia coli ED1a RepID...    98   6e-20

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_Q9F505 YheB protein n=2 Tax=Escherichia coli RepID=Q9F505_ECOLX
          Length = 71

 Score =  108 bits (270), Expect = 4e-23,   Method: Composition-based stats.
 Identities = 25/60 (41%), Positives = 34/60 (56%)

Query: 2  YKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQS 61
          Y+I ATI+K GG P  WT YS   +T  EC K  S +KEAG      V + +F C+++  
Sbjct: 4  YQINATIKKPGGGPVKWTHYSDFPMTAEECRKNFSKEKEAGKCFSVDVHISDFICKEIPP 63


>UniRef50_P33230 Uncharacterized protein ydaC n=26 Tax=Enterobacteriaceae
          RepID=YDAC_ECOLI
          Length = 69

 Score =  104 bits (260), Expect = 7e-22,   Method: Composition-based stats.
 Identities = 69/69 (100%), Positives = 69/69 (100%)

Query: 1  MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQ 60
          MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQ
Sbjct: 1  MYKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQ 60

Query: 61 SSRIALYSN 69
          SSRIALYSN
Sbjct: 61 SSRIALYSN 69


>UniRef50_B7MZY6 YheB protein n=1 Tax=Escherichia coli ED1a RepID=B7MZY6_ECO81
          Length = 76

 Score = 98.3 bits (243), Expect = 6e-20,   Method: Composition-based stats.
 Identities = 29/61 (47%), Positives = 40/61 (65%)

Query: 2  YKITATIEKEGGTPTNWTRYSKSKLTKSECEKMLSGKKEAGVSREQKVKLINFNCEKLQS 61
          Y I ATIEK GGTP +W R+  + +TK ECEK+ S +KEAG S   KV +  F+C++   
Sbjct: 13 YCIQATIEKPGGTPVSWIRFQNTPMTKQECEKLFSTEKEAGKSFTVKVGVTQFSCKENPP 72

Query: 62 S 62
          +
Sbjct: 73 A 73


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.304    0.124    0.353 

Lambda     K      H
   0.267   0.0388    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 240,602,752
Number of Sequences: 3077464
Number of extensions: 6186471
Number of successful extensions: 13904
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 13898
Number of HSP's gapped (non-prelim): 6
length of query: 69
length of database: 1,040,396,356
effective HSP length: 41
effective length of query: 28
effective length of database: 914,220,332
effective search space: 25598169296
effective search space used: 25598169296
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.5 bits)
S2: 87 (38.2 bits)