Processing 1 helix: Transmembrane helix 1 from 10 (in) to 25 (out) : -377.495 Score = -0.382958 Processing 1 helix: Transmembrane helix 1 from 10 (out) to 25 (in) : -373.605 Score = -0.368142 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -556.814 Transmembrane helix 2 from 23 (out) to 38 (in) : -830.199 Transmembrane helix 3 from 43 (in) to 58 (out) : -1066.87 Transmembrane helix 4 from 62 (out) to 77 (in) : -891.012 Transmembrane helix 5 from 84 (in) to 99 (out) : -988.152 Transmembrane helix 6 from 106 (out) to 121 (in) : -699.104 Transmembrane helix 7 from 125 (in) to 140 (out) : -793.876 Transmembrane helix 8 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -549.486 Transmembrane helix 2 from 23 (in) to 38 (out) : -828.901 Transmembrane helix 3 from 61 (out) to 76 (in) : -888.364 Transmembrane helix 4 from 84 (in) to 99 (out) : -988.152 Transmembrane helix 5 from 106 (out) to 121 (in) : -699.104 Transmembrane helix 6 from 125 (in) to 140 (out) : -793.876 Transmembrane helix 7 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -377.495 Transmembrane helix 2 from 61 (out) to 76 (in) : -888.364 Transmembrane helix 3 from 84 (in) to 99 (out) : -988.152 Transmembrane helix 4 from 106 (out) to 121 (in) : -699.104 Transmembrane helix 5 from 125 (in) to 140 (out) : -793.876 Transmembrane helix 6 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -373.605 Transmembrane helix 2 from 61 (in) to 76 (out) : -888.236 Transmembrane helix 3 from 106 (out) to 121 (in) : -699.104 Transmembrane helix 4 from 125 (in) to 140 (out) : -793.876 Transmembrane helix 5 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -377.495 Transmembrane helix 2 from 61 (out) to 76 (in) : -888.364 Transmembrane helix 3 from 115 (in) to 130 (out) : -600.67 Transmembrane helix 4 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -373.605 Transmembrane helix 2 from 115 (in) to 130 (out) : -600.67 Transmembrane helix 3 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -377.495 Transmembrane helix 2 from 145 (out) to 160 (in) : -548.777 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -549.486 Transmembrane helix 2 from 23 (in) to 38 (out) : -828.901 Transmembrane helix 3 from 43 (out) to 58 (in) : -1060.63 Transmembrane helix 4 from 62 (in) to 77 (out) : -891.488 Transmembrane helix 5 from 84 (out) to 99 (in) : -980.448 Transmembrane helix 6 from 106 (in) to 121 (out) : -692.396 Transmembrane helix 7 from 125 (out) to 140 (in) : -790.624 Transmembrane helix 8 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -556.814 Transmembrane helix 2 from 23 (out) to 38 (in) : -830.199 Transmembrane helix 3 from 61 (in) to 76 (out) : -888.236 Transmembrane helix 4 from 84 (out) to 99 (in) : -980.448 Transmembrane helix 5 from 106 (in) to 121 (out) : -692.396 Transmembrane helix 6 from 125 (out) to 140 (in) : -790.624 Transmembrane helix 7 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -373.605 Transmembrane helix 2 from 61 (in) to 76 (out) : -888.236 Transmembrane helix 3 from 84 (out) to 99 (in) : -980.448 Transmembrane helix 4 from 106 (in) to 121 (out) : -692.396 Transmembrane helix 5 from 125 (out) to 140 (in) : -790.624 Transmembrane helix 6 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -377.495 Transmembrane helix 2 from 61 (out) to 76 (in) : -888.364 Transmembrane helix 3 from 106 (in) to 121 (out) : -692.396 Transmembrane helix 4 from 125 (out) to 140 (in) : -790.624 Transmembrane helix 5 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -373.605 Transmembrane helix 2 from 106 (in) to 121 (out) : -692.396 Transmembrane helix 3 from 125 (out) to 140 (in) : -790.624 Transmembrane helix 4 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : -377.495 Transmembrane helix 2 from 116 (out) to 131 (in) : -606.013 Transmembrane helix 3 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : -373.605 Transmembrane helix 2 from 145 (in) to 160 (out) : -545.223 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.382958 1 helix (-) : Score = -0.368142 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 10-25 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.368142