BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (46 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P64443 Uncharacterized protein yceO n=85 Tax=Enterobact...    90   2e-17
UniRef50_D2TT64 Putative membrane protein n=1 Tax=Citrobacter ro...    54   1e-06

>UniRef50_P64443 Uncharacterized protein yceO n=85 Tax=Enterobacteriaceae
          RepID=YCEO_ECO57
          Length = 46

 Score = 89.7 bits (221), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 1  MRPFLQEYLMRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF 46
          MRPFLQEYLMRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF
Sbjct: 1  MRPFLQEYLMRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF 46


>UniRef50_D2TT64 Putative membrane protein n=1 Tax=Citrobacter rodentium ICC168
          RepID=D2TT64_CITRO
          Length = 37

 Score = 54.3 bits (129), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 23/37 (62%), Positives = 32/37 (86%)

Query: 10 MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF 46
          MRRL  + +NNIR+H MLY+FLW LLAI+D+IY+++F
Sbjct: 1  MRRLFQFWVNNIRQHFMLYIFLWSLLAILDVIYIYFF 37


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P64443 Uncharacterized protein yceO n=85 Tax=Enterobact...    48   1e-04

Sequences not found previously or not previously below threshold:

UniRef50_D2TT64 Putative membrane protein n=1 Tax=Citrobacter ro...    39   0.037


CONVERGED!
>UniRef50_P64443 Uncharacterized protein yceO n=85 Tax=Enterobacteriaceae
          RepID=YCEO_ECO57
          Length = 46

 Score = 47.8 bits (113), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 1  MRPFLQEYLMRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF 46
          MRPFLQEYLMRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF
Sbjct: 1  MRPFLQEYLMRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF 46


>UniRef50_D2TT64 Putative membrane protein n=1 Tax=Citrobacter rodentium ICC168
          RepID=D2TT64_CITRO
          Length = 37

 Score = 39.3 bits (91), Expect = 0.037,   Method: Composition-based stats.
 Identities = 23/37 (62%), Positives = 32/37 (86%)

Query: 10 MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF 46
          MRRL  + +NNIR+H MLY+FLW LLAI+D+IY+++F
Sbjct: 1  MRRLFQFWVNNIRQHFMLYIFLWSLLAILDVIYIYFF 37


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.346    0.168    0.565 

Lambda     K      H
   0.267   0.0510    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 236,366,665
Number of Sequences: 3077464
Number of extensions: 7735321
Number of successful extensions: 53535
Number of sequences better than 1.0e-01: 4
Number of HSP's better than  0.1 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 53527
Number of HSP's gapped (non-prelim): 8
length of query: 46
length of database: 1,040,396,356
effective HSP length: 20
effective length of query: 26
effective length of database: 978,847,076
effective search space: 25450023976
effective search space used: 25450023976
T: 11
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.6 bits)
S2: 88 (38.2 bits)