BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (92 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P75711 Uncharacterized protein ybbV n=7 Tax=Escherichia...   186   2e-46
UniRef50_D0ZP16 Putative cytoplasmic protein n=2 Tax=Salmonella ...    62   7e-09

>UniRef50_P75711 Uncharacterized protein ybbV n=7 Tax=Escherichia coli
          RepID=YBBV_ECOLI
          Length = 92

 Score =  186 bits (472), Expect = 2e-46,   Method: Compositional matrix adjust.
 Identities = 92/92 (100%), Positives = 92/92 (100%)

Query: 1  MNRPAILKKKAAKDVASVLKIIFLFYLFLIARLKQRYSIREIKRDLWNIRENYSSNAAIA 60
          MNRPAILKKKAAKDVASVLKIIFLFYLFLIARLKQRYSIREIKRDLWNIRENYSSNAAIA
Sbjct: 1  MNRPAILKKKAAKDVASVLKIIFLFYLFLIARLKQRYSIREIKRDLWNIRENYSSNAAIA 60

Query: 61 KIYCRKRKASGPGKHLTILPYGWVRFITFPIM 92
          KIYCRKRKASGPGKHLTILPYGWVRFITFPIM
Sbjct: 61 KIYCRKRKASGPGKHLTILPYGWVRFITFPIM 92


>UniRef50_D0ZP16 Putative cytoplasmic protein n=2 Tax=Salmonella enterica subsp.
          enterica serovar Typhimurium RepID=D0ZP16_SALT1
          Length = 84

 Score = 61.6 bits (148), Expect = 7e-09,   Method: Compositional matrix adjust.
 Identities = 30/53 (56%), Positives = 40/53 (75%), Gaps = 1/53 (1%)

Query: 39 IREIKRDLWNIRENYSSNAAIAKIYCRKRKASGPGKHLTILPYGWVRFITFPI 91
          +RE KRDLWNI+E+Y+S+A IAK Y  ++K +  G+HLTI PYGW  +IT  I
Sbjct: 1  MRE-KRDLWNIKESYTSSAVIAKTYYLRQKPNETGRHLTISPYGWDLYITCQI 52


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P75711 Uncharacterized protein ybbV n=7 Tax=Escherichia...   155   6e-37
UniRef50_D0ZP16 Putative cytoplasmic protein n=2 Tax=Salmonella ...    88   1e-16

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P75711 Uncharacterized protein ybbV n=7 Tax=Escherichia coli
          RepID=YBBV_ECOLI
          Length = 92

 Score =  155 bits (391), Expect = 6e-37,   Method: Composition-based stats.
 Identities = 92/92 (100%), Positives = 92/92 (100%)

Query: 1  MNRPAILKKKAAKDVASVLKIIFLFYLFLIARLKQRYSIREIKRDLWNIRENYSSNAAIA 60
          MNRPAILKKKAAKDVASVLKIIFLFYLFLIARLKQRYSIREIKRDLWNIRENYSSNAAIA
Sbjct: 1  MNRPAILKKKAAKDVASVLKIIFLFYLFLIARLKQRYSIREIKRDLWNIRENYSSNAAIA 60

Query: 61 KIYCRKRKASGPGKHLTILPYGWVRFITFPIM 92
          KIYCRKRKASGPGKHLTILPYGWVRFITFPIM
Sbjct: 61 KIYCRKRKASGPGKHLTILPYGWVRFITFPIM 92


>UniRef50_D0ZP16 Putative cytoplasmic protein n=2 Tax=Salmonella enterica subsp.
          enterica serovar Typhimurium RepID=D0ZP16_SALT1
          Length = 84

 Score = 87.8 bits (216), Expect = 1e-16,   Method: Composition-based stats.
 Identities = 30/53 (56%), Positives = 40/53 (75%), Gaps = 1/53 (1%)

Query: 39 IREIKRDLWNIRENYSSNAAIAKIYCRKRKASGPGKHLTILPYGWVRFITFPI 91
          +RE KRDLWNI+E+Y+S+A IAK Y  ++K +  G+HLTI PYGW  +IT  I
Sbjct: 1  MRE-KRDLWNIKESYTSSAVIAKTYYLRQKPNETGRHLTISPYGWDLYITCQI 52


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.324    0.140    0.409 

Lambda     K      H
   0.267   0.0421    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 368,847,610
Number of Sequences: 3077464
Number of extensions: 12039726
Number of successful extensions: 37315
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 37311
Number of HSP's gapped (non-prelim): 4
length of query: 92
length of database: 1,040,396,356
effective HSP length: 62
effective length of query: 30
effective length of database: 849,593,588
effective search space: 25487807640
effective search space used: 25487807640
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 87 (38.1 bits)