BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (62 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q79AM8 Orf62 n=2 Tax=Escherichia coli RepID=Q79AM8_ECOLX     127   9e-29

>UniRef50_Q79AM8 Orf62 n=2 Tax=Escherichia coli RepID=Q79AM8_ECOLX
          Length = 62

 Score =  127 bits (320), Expect = 9e-29,   Method: Compositional matrix adjust.
 Identities = 62/62 (100%), Positives = 62/62 (100%)

Query: 1  MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFRFPAFFSDPTVNSLFSHTEAFSQFFGGHF 60
          MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFRFPAFFSDPTVNSLFSHTEAFSQFFGGHF
Sbjct: 1  MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFRFPAFFSDPTVNSLFSHTEAFSQFFGGHF 60

Query: 61 IA 62
          IA
Sbjct: 61 IA 62


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q79AM8 Orf62 n=2 Tax=Escherichia coli RepID=Q79AM8_ECOLX     107   1e-22

Sequences not found previously or not previously below threshold:

UniRef50_B4RS49 Putative lipopolysaccharide modification acyltra...    38   0.078


CONVERGED!
>UniRef50_Q79AM8 Orf62 n=2 Tax=Escherichia coli RepID=Q79AM8_ECOLX
          Length = 62

 Score =  107 bits (267), Expect = 1e-22,   Method: Composition-based stats.
 Identities = 62/62 (100%), Positives = 62/62 (100%)

Query: 1  MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFRFPAFFSDPTVNSLFSHTEAFSQFFGGHF 60
          MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFRFPAFFSDPTVNSLFSHTEAFSQFFGGHF
Sbjct: 1  MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFRFPAFFSDPTVNSLFSHTEAFSQFFGGHF 60

Query: 61 IA 62
          IA
Sbjct: 61 IA 62


>UniRef50_B4RS49 Putative lipopolysaccharide modification acyltransferase n=1
           Tax=Alteromonas macleodii 'Deep ecotype'
           RepID=B4RS49_ALTMD
          Length = 639

 Score = 38.5 bits (88), Expect = 0.078,   Method: Composition-based stats.
 Identities = 23/61 (37%), Positives = 36/61 (59%), Gaps = 6/61 (9%)

Query: 1   MEGWRVLNKLANLRIDQNKSGTTAFHAGKFFR-FPAFFSDPTVNSLFSHTEAFSQFFGGH 59
           + G+ ++ ++ N +I QN    + F+  +F R FPAFF+  +V+SLF    AFS F  G 
Sbjct: 65  ISGYLIMGQIYN-KIQQNAFSISDFYVKRFKRLFPAFFATVSVSSLF----AFSYFLPGE 119

Query: 60  F 60
           F
Sbjct: 120 F 120


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.328    0.139    0.442 

Lambda     K      H
   0.267   0.0435    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 296,588,652
Number of Sequences: 3077464
Number of extensions: 10095137
Number of successful extensions: 26042
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 26040
Number of HSP's gapped (non-prelim): 3
length of query: 62
length of database: 1,040,396,356
effective HSP length: 34
effective length of query: 28
effective length of database: 935,762,580
effective search space: 26201352240
effective search space used: 26201352240
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 87 (38.0 bits)