Processing 1 helix: Transmembrane helix 1 from 276 (in) to 306 (out) : 3757.77 Score = 3.756743 Processing 1 helix: Transmembrane helix 1 from 276 (out) to 306 (in) : 3798.53 Score = 3.799557 Processing 16 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 115 (in) : 3204.09 Transmembrane helix 7 from 119 (in) to 134 (out) : -417.09 Transmembrane helix 8 from 138 (out) to 153 (in) : -1470.61 Transmembrane helix 9 from 157 (in) to 172 (out) : -1532.28 Transmembrane helix 10 from 176 (out) to 191 (in) : -1628.47 Transmembrane helix 11 from 195 (in) to 210 (out) : -1686.66 Transmembrane helix 12 from 214 (out) to 229 (in) : -1630.22 Transmembrane helix 13 from 233 (in) to 248 (out) : -1242.62 Transmembrane helix 14 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 15 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 16 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 115 (out) : 3218.51 Transmembrane helix 7 from 119 (out) to 134 (in) : -414.31 Transmembrane helix 8 from 138 (in) to 153 (out) : -1467.09 Transmembrane helix 9 from 157 (out) to 172 (in) : -1526.42 Transmembrane helix 10 from 184 (in) to 199 (out) : -1472.84 Transmembrane helix 11 from 214 (out) to 229 (in) : -1630.22 Transmembrane helix 12 from 233 (in) to 248 (out) : -1242.62 Transmembrane helix 13 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 14 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 15 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 115 (in) : 3204.09 Transmembrane helix 7 from 119 (in) to 134 (out) : -417.09 Transmembrane helix 8 from 138 (out) to 153 (in) : -1470.61 Transmembrane helix 9 from 157 (in) to 172 (out) : -1532.28 Transmembrane helix 10 from 184 (out) to 199 (in) : -1489.06 Transmembrane helix 11 from 229 (in) to 244 (out) : -1229.98 Transmembrane helix 12 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 13 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 14 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 116 (out) : 3359.46 Transmembrane helix 7 from 120 (out) to 135 (in) : -535.996 Transmembrane helix 8 from 139 (in) to 154 (out) : -1483.75 Transmembrane helix 9 from 184 (out) to 199 (in) : -1489.06 Transmembrane helix 10 from 229 (in) to 244 (out) : -1229.98 Transmembrane helix 11 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 12 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 13 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 116 (in) : 3344.34 Transmembrane helix 7 from 120 (in) to 135 (out) : -535.704 Transmembrane helix 8 from 139 (out) to 154 (in) : -1487.75 Transmembrane helix 9 from 229 (in) to 244 (out) : -1229.98 Transmembrane helix 10 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 11 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 12 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 116 (out) : 3359.46 Transmembrane helix 7 from 120 (out) to 135 (in) : -535.996 Transmembrane helix 8 from 229 (in) to 244 (out) : -1229.98 Transmembrane helix 9 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 10 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 11 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 116 (in) : 3344.34 Transmembrane helix 7 from 120 (in) to 135 (out) : -535.704 Transmembrane helix 8 from 252 (out) to 267 (in) : -1021.95 Transmembrane helix 9 from 271 (in) to 286 (out) : 3304.95 Transmembrane helix 10 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 116 (out) : 3359.46 Transmembrane helix 7 from 120 (out) to 135 (in) : -535.996 Transmembrane helix 8 from 268 (in) to 286 (out) : 3451.58 Transmembrane helix 9 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 121 (in) : 3570.03 Transmembrane helix 7 from 268 (in) to 286 (out) : 3451.58 Transmembrane helix 8 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 11 (out) to 32 (in) : 3287.72 Transmembrane helix 2 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 3 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 4 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 5 from 100 (out) to 121 (in) : 3570.03 Transmembrane helix 6 from 268 (in) to 286 (out) : 3451.58 Transmembrane helix 7 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 11 (in) to 32 (out) : 3291.88 Transmembrane helix 2 from 47 (out) to 77 (in) : 2766.33 Transmembrane helix 3 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 4 from 100 (out) to 121 (in) : 3570.03 Transmembrane helix 5 from 268 (in) to 286 (out) : 3451.58 Transmembrane helix 6 from 290 (out) to 306 (in) : 1323.14 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 11 (out) to 32 (in) : 3287.72 Transmembrane helix 2 from 47 (in) to 77 (out) : 2732.47 Transmembrane helix 3 from 90 (out) to 120 (in) : 3691.47 Transmembrane helix 4 from 268 (in) to 286 (out) : 3451.58 Transmembrane helix 5 from 290 (out) to 306 (in) : 1323.14 Score = 14.450351 Processing 4 helices: Transmembrane helix 1 from 11 (in) to 32 (out) : 3291.88 Transmembrane helix 2 from 47 (out) to 77 (in) : 2766.33 Transmembrane helix 3 from 90 (in) to 120 (out) : 3727.33 Transmembrane helix 4 from 276 (out) to 306 (in) : 3798.53 Score = 13.621914 Processing 3 helices: Transmembrane helix 1 from 11 (out) to 32 (in) : 3287.72 Transmembrane helix 2 from 96 (in) to 121 (out) : 3701.21 Transmembrane helix 3 from 276 (out) to 306 (in) : 3798.53 Score = 10.786959 Processing 2 helices: Transmembrane helix 1 from 90 (in) to 120 (out) : 3727.33 Transmembrane helix 2 from 276 (out) to 306 (in) : 3798.53 Score = 7.526887 Processing 16 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 115 (out) : 3218.51 Transmembrane helix 7 from 119 (out) to 134 (in) : -414.31 Transmembrane helix 8 from 138 (in) to 153 (out) : -1467.09 Transmembrane helix 9 from 157 (out) to 172 (in) : -1526.42 Transmembrane helix 10 from 176 (in) to 191 (out) : -1616.63 Transmembrane helix 11 from 195 (out) to 210 (in) : -1688.14 Transmembrane helix 12 from 214 (in) to 229 (out) : -1631.98 Transmembrane helix 13 from 233 (out) to 248 (in) : -1242.28 Transmembrane helix 14 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 15 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 16 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 115 (in) : 3204.09 Transmembrane helix 7 from 119 (in) to 134 (out) : -417.09 Transmembrane helix 8 from 138 (out) to 153 (in) : -1470.61 Transmembrane helix 9 from 157 (in) to 172 (out) : -1532.28 Transmembrane helix 10 from 184 (out) to 199 (in) : -1489.06 Transmembrane helix 11 from 214 (in) to 229 (out) : -1631.98 Transmembrane helix 12 from 233 (out) to 248 (in) : -1242.28 Transmembrane helix 13 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 14 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 15 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 115 (out) : 3218.51 Transmembrane helix 7 from 119 (out) to 134 (in) : -414.31 Transmembrane helix 8 from 138 (in) to 153 (out) : -1467.09 Transmembrane helix 9 from 157 (out) to 172 (in) : -1526.42 Transmembrane helix 10 from 184 (in) to 199 (out) : -1472.84 Transmembrane helix 11 from 230 (out) to 245 (in) : -1226.23 Transmembrane helix 12 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 13 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 14 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 116 (in) : 3344.34 Transmembrane helix 7 from 120 (in) to 135 (out) : -535.704 Transmembrane helix 8 from 157 (out) to 172 (in) : -1526.42 Transmembrane helix 9 from 184 (in) to 199 (out) : -1472.84 Transmembrane helix 10 from 230 (out) to 245 (in) : -1226.23 Transmembrane helix 11 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 12 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 13 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 116 (out) : 3359.46 Transmembrane helix 7 from 120 (out) to 135 (in) : -535.996 Transmembrane helix 8 from 184 (in) to 199 (out) : -1472.84 Transmembrane helix 9 from 230 (out) to 245 (in) : -1226.23 Transmembrane helix 10 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 11 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 12 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 116 (in) : 3344.34 Transmembrane helix 7 from 120 (in) to 135 (out) : -535.704 Transmembrane helix 8 from 229 (out) to 244 (in) : -1225.32 Transmembrane helix 9 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 10 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 11 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 116 (out) : 3359.46 Transmembrane helix 7 from 120 (out) to 135 (in) : -535.996 Transmembrane helix 8 from 252 (in) to 267 (out) : -1023.65 Transmembrane helix 9 from 271 (out) to 286 (in) : 3308.05 Transmembrane helix 10 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 907.927 Transmembrane helix 2 from 23 (out) to 38 (in) : 1742.66 Transmembrane helix 3 from 43 (in) to 58 (out) : 699.433 Transmembrane helix 4 from 62 (out) to 77 (in) : 1763.47 Transmembrane helix 5 from 81 (in) to 96 (out) : 13.279 Transmembrane helix 6 from 100 (out) to 116 (in) : 3344.34 Transmembrane helix 7 from 120 (in) to 135 (out) : -535.704 Transmembrane helix 8 from 268 (out) to 286 (in) : 3451.62 Transmembrane helix 9 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 898.873 Transmembrane helix 2 from 23 (in) to 38 (out) : 1754.94 Transmembrane helix 3 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 4 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 5 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 6 from 100 (in) to 121 (out) : 3589.57 Transmembrane helix 7 from 268 (out) to 286 (in) : 3451.62 Transmembrane helix 8 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 11 (in) to 32 (out) : 3291.88 Transmembrane helix 2 from 43 (out) to 58 (in) : 708.167 Transmembrane helix 3 from 62 (in) to 77 (out) : 1734.53 Transmembrane helix 4 from 81 (out) to 96 (in) : 13.021 Transmembrane helix 5 from 100 (in) to 121 (out) : 3589.57 Transmembrane helix 6 from 268 (out) to 286 (in) : 3451.62 Transmembrane helix 7 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 11 (out) to 32 (in) : 3287.72 Transmembrane helix 2 from 47 (in) to 76 (out) : 2706 Transmembrane helix 3 from 80 (out) to 95 (in) : -12.203 Transmembrane helix 4 from 99 (in) to 121 (out) : 3644.63 Transmembrane helix 5 from 268 (out) to 286 (in) : 3451.62 Transmembrane helix 6 from 290 (in) to 306 (out) : 1325.16 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 11 (in) to 32 (out) : 3291.88 Transmembrane helix 2 from 47 (out) to 77 (in) : 2766.33 Transmembrane helix 3 from 90 (in) to 120 (out) : 3727.33 Transmembrane helix 4 from 268 (out) to 286 (in) : 3451.62 Transmembrane helix 5 from 290 (in) to 306 (out) : 1325.16 Score = 14.598351 Processing 4 helices: Transmembrane helix 1 from 11 (out) to 32 (in) : 3287.72 Transmembrane helix 2 from 47 (in) to 77 (out) : 2732.47 Transmembrane helix 3 from 90 (out) to 120 (in) : 3691.47 Transmembrane helix 4 from 276 (in) to 306 (out) : 3757.77 Score = 13.431585 Processing 3 helices: Transmembrane helix 1 from 11 (in) to 32 (out) : 3291.88 Transmembrane helix 2 from 90 (out) to 120 (in) : 3691.47 Transmembrane helix 3 from 276 (in) to 306 (out) : 3757.77 Score = 10.806023 Processing 2 helices: Transmembrane helix 1 from 90 (out) to 120 (in) : 3691.47 Transmembrane helix 2 from 276 (in) to 306 (out) : 3757.77 Score = 7.448214 Summary of topology analysis: 1 helix (+) : Score = 3.75674 1 helix (-) : Score = 3.79956 2 helices (+) : Score = 7.52689 2 helices (-) : Score = 7.44821 3 helices (+) : Score = 10.806 3 helices (-) : Score = 10.787 4 helices (+) : Score = 13.6219 4 helices (-) : Score = 13.4316 5 helices (+) : Score = 14.5984 5 helices (-) : Score = 14.4504 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 11-32,47-77,90-120,268-286,290-306 Re-entrant helices: Not detected. Helix count: 5 N-terminal: in Score: 14.5984