Processing 1 helix: Transmembrane helix 1 from 296 (in) to 311 (out) : 124.004 Score = 0.124004 Processing 1 helix: Transmembrane helix 1 from 295 (out) to 310 (in) : 130.773 Score = 0.130773 Processing 22 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -75.354 Transmembrane helix 2 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 3 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 4 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 5 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 6 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 7 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 8 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 9 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 10 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 11 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 12 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 13 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 14 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 15 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 16 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 17 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 18 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 19 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 20 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 21 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 22 from 1 (out) to 0 (in) : -4.841 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 22 (out) to 37 (in) : -306.954 Transmembrane helix 2 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 3 from 60 (out) to 75 (in) : -218.527 Transmembrane helix 4 from 79 (in) to 94 (out) : -319.111 Transmembrane helix 5 from 98 (out) to 113 (in) : -178.237 Transmembrane helix 6 from 117 (in) to 132 (out) : -141.314 Transmembrane helix 7 from 136 (out) to 151 (in) : -127.497 Transmembrane helix 8 from 155 (in) to 170 (out) : -598.665 Transmembrane helix 9 from 174 (out) to 189 (in) : -784.941 Transmembrane helix 10 from 193 (in) to 208 (out) : -98.806 Transmembrane helix 11 from 212 (out) to 227 (in) : -136.566 Transmembrane helix 12 from 231 (in) to 246 (out) : -10.91 Transmembrane helix 13 from 250 (out) to 265 (in) : -33.97 Transmembrane helix 14 from 269 (in) to 284 (out) : -51.736 Transmembrane helix 15 from 288 (out) to 303 (in) : -22.097 Transmembrane helix 16 from 307 (in) to 322 (out) : -264.314 Transmembrane helix 17 from 326 (out) to 341 (in) : -515.316 Transmembrane helix 18 from 345 (in) to 360 (out) : -230.849 Transmembrane helix 19 from 364 (out) to 379 (in) : -395.367 Transmembrane helix 20 from 383 (in) to 398 (out) : -154.947 Transmembrane helix 21 from 402 (out) to 417 (in) : -148.012 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -246.519 Transmembrane helix 2 from 43 (out) to 58 (in) : -40.383 Transmembrane helix 3 from 62 (in) to 77 (out) : -204.513 Transmembrane helix 4 from 81 (out) to 96 (in) : -305.392 Transmembrane helix 5 from 100 (in) to 115 (out) : -156.69 Transmembrane helix 6 from 119 (out) to 134 (in) : -138.58 Transmembrane helix 7 from 138 (in) to 153 (out) : -168.386 Transmembrane helix 8 from 159 (out) to 174 (in) : -587.884 Transmembrane helix 9 from 193 (in) to 208 (out) : -98.806 Transmembrane helix 10 from 212 (out) to 227 (in) : -136.566 Transmembrane helix 11 from 231 (in) to 246 (out) : -10.91 Transmembrane helix 12 from 250 (out) to 265 (in) : -33.97 Transmembrane helix 13 from 269 (in) to 284 (out) : -51.736 Transmembrane helix 14 from 288 (out) to 303 (in) : -22.097 Transmembrane helix 15 from 307 (in) to 322 (out) : -264.314 Transmembrane helix 16 from 326 (out) to 341 (in) : -515.316 Transmembrane helix 17 from 345 (in) to 360 (out) : -230.849 Transmembrane helix 18 from 364 (out) to 379 (in) : -395.367 Transmembrane helix 19 from 383 (in) to 398 (out) : -154.947 Transmembrane helix 20 from 402 (out) to 417 (in) : -148.012 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -212.686 Transmembrane helix 2 from 44 (in) to 59 (out) : -60.948 Transmembrane helix 3 from 63 (out) to 78 (in) : -216.746 Transmembrane helix 4 from 82 (in) to 97 (out) : -292.965 Transmembrane helix 5 from 101 (out) to 116 (in) : -136.978 Transmembrane helix 6 from 120 (in) to 135 (out) : -144.424 Transmembrane helix 7 from 139 (out) to 154 (in) : -203.33 Transmembrane helix 8 from 193 (in) to 208 (out) : -98.806 Transmembrane helix 9 from 212 (out) to 227 (in) : -136.566 Transmembrane helix 10 from 231 (in) to 246 (out) : -10.91 Transmembrane helix 11 from 250 (out) to 265 (in) : -33.97 Transmembrane helix 12 from 269 (in) to 284 (out) : -51.736 Transmembrane helix 13 from 288 (out) to 303 (in) : -22.097 Transmembrane helix 14 from 307 (in) to 322 (out) : -264.314 Transmembrane helix 15 from 326 (out) to 341 (in) : -515.316 Transmembrane helix 16 from 345 (in) to 360 (out) : -230.849 Transmembrane helix 17 from 364 (out) to 379 (in) : -395.367 Transmembrane helix 18 from 383 (in) to 398 (out) : -154.947 Transmembrane helix 19 from 402 (out) to 417 (in) : -148.012 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -246.519 Transmembrane helix 2 from 43 (out) to 58 (in) : -40.383 Transmembrane helix 3 from 62 (in) to 77 (out) : -204.513 Transmembrane helix 4 from 81 (out) to 96 (in) : -305.392 Transmembrane helix 5 from 100 (in) to 115 (out) : -156.69 Transmembrane helix 6 from 119 (out) to 134 (in) : -138.58 Transmembrane helix 7 from 138 (in) to 153 (out) : -168.386 Transmembrane helix 8 from 198 (out) to 213 (in) : 38.344 Transmembrane helix 9 from 217 (in) to 232 (out) : -130.924 Transmembrane helix 10 from 236 (out) to 251 (in) : -15.735 Transmembrane helix 11 from 255 (in) to 270 (out) : -11.866 Transmembrane helix 12 from 274 (out) to 289 (in) : -164.437 Transmembrane helix 13 from 293 (in) to 308 (out) : 102.644 Transmembrane helix 14 from 312 (out) to 327 (in) : -431.873 Transmembrane helix 15 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 16 from 364 (out) to 379 (in) : -395.367 Transmembrane helix 17 from 383 (in) to 398 (out) : -154.947 Transmembrane helix 18 from 402 (out) to 417 (in) : -148.012 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -212.686 Transmembrane helix 2 from 44 (in) to 59 (out) : -60.948 Transmembrane helix 3 from 63 (out) to 78 (in) : -216.746 Transmembrane helix 4 from 82 (in) to 97 (out) : -292.965 Transmembrane helix 5 from 101 (out) to 116 (in) : -136.978 Transmembrane helix 6 from 120 (in) to 135 (out) : -144.424 Transmembrane helix 7 from 139 (out) to 154 (in) : -203.33 Transmembrane helix 8 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 9 from 219 (out) to 234 (in) : -108.471 Transmembrane helix 10 from 238 (in) to 253 (out) : -14.568 Transmembrane helix 11 from 257 (out) to 272 (in) : 14.587 Transmembrane helix 12 from 276 (in) to 291 (out) : -181.891 Transmembrane helix 13 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 14 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 15 from 364 (out) to 379 (in) : -395.367 Transmembrane helix 16 from 383 (in) to 398 (out) : -154.947 Transmembrane helix 17 from 402 (out) to 417 (in) : -148.012 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -246.519 Transmembrane helix 2 from 43 (out) to 58 (in) : -40.383 Transmembrane helix 3 from 62 (in) to 77 (out) : -204.513 Transmembrane helix 4 from 81 (out) to 96 (in) : -305.392 Transmembrane helix 5 from 100 (in) to 115 (out) : -156.69 Transmembrane helix 6 from 119 (out) to 134 (in) : -138.58 Transmembrane helix 7 from 138 (in) to 153 (out) : -168.386 Transmembrane helix 8 from 199 (out) to 214 (in) : 49.7 Transmembrane helix 9 from 218 (in) to 233 (out) : -125.633 Transmembrane helix 10 from 237 (out) to 252 (in) : -16.924 Transmembrane helix 11 from 256 (in) to 271 (out) : -7.305 Transmembrane helix 12 from 275 (out) to 290 (in) : -179.147 Transmembrane helix 13 from 296 (in) to 311 (out) : 124.004 Transmembrane helix 14 from 343 (out) to 358 (in) : -211.271 Transmembrane helix 15 from 374 (in) to 389 (out) : -120.926 Transmembrane helix 16 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -189.556 Transmembrane helix 2 from 45 (in) to 60 (out) : -72.186 Transmembrane helix 3 from 64 (out) to 79 (in) : -228.122 Transmembrane helix 4 from 86 (in) to 101 (out) : -253.591 Transmembrane helix 5 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 6 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 7 from 199 (out) to 214 (in) : 49.7 Transmembrane helix 8 from 218 (in) to 233 (out) : -125.633 Transmembrane helix 9 from 237 (out) to 252 (in) : -16.924 Transmembrane helix 10 from 256 (in) to 271 (out) : -7.305 Transmembrane helix 11 from 275 (out) to 290 (in) : -179.147 Transmembrane helix 12 from 296 (in) to 311 (out) : 124.004 Transmembrane helix 13 from 343 (out) to 358 (in) : -211.271 Transmembrane helix 14 from 374 (in) to 389 (out) : -120.926 Transmembrane helix 15 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 25 (in) to 40 (out) : -225.514 Transmembrane helix 2 from 44 (out) to 59 (in) : -51.452 Transmembrane helix 3 from 63 (in) to 78 (out) : -211.054 Transmembrane helix 4 from 86 (out) to 101 (in) : -250.809 Transmembrane helix 5 from 110 (in) to 125 (out) : -98.372 Transmembrane helix 6 from 130 (out) to 145 (in) : -82.195 Transmembrane helix 7 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 8 from 219 (out) to 234 (in) : -108.471 Transmembrane helix 9 from 238 (in) to 253 (out) : -14.568 Transmembrane helix 10 from 257 (out) to 272 (in) : 14.587 Transmembrane helix 11 from 276 (in) to 291 (out) : -181.891 Transmembrane helix 12 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 13 from 373 (in) to 388 (out) : -130.573 Transmembrane helix 14 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 60 (in) to 75 (out) : -203.873 Transmembrane helix 3 from 86 (out) to 101 (in) : -250.809 Transmembrane helix 4 from 110 (in) to 125 (out) : -98.372 Transmembrane helix 5 from 130 (out) to 145 (in) : -82.195 Transmembrane helix 6 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 7 from 219 (out) to 234 (in) : -108.471 Transmembrane helix 8 from 238 (in) to 253 (out) : -14.568 Transmembrane helix 9 from 257 (out) to 272 (in) : 14.587 Transmembrane helix 10 from 276 (in) to 291 (out) : -181.891 Transmembrane helix 11 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 12 from 373 (in) to 388 (out) : -130.573 Transmembrane helix 13 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 25 (in) to 40 (out) : -225.514 Transmembrane helix 2 from 44 (out) to 59 (in) : -51.452 Transmembrane helix 3 from 63 (in) to 78 (out) : -211.054 Transmembrane helix 4 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 5 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 6 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 7 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 8 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 9 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 10 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 11 from 373 (in) to 388 (out) : -130.573 Transmembrane helix 12 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 60 (in) to 75 (out) : -203.873 Transmembrane helix 3 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 4 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 5 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 6 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 7 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 8 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 9 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 10 from 373 (in) to 388 (out) : -130.573 Transmembrane helix 11 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 3 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 4 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 5 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 6 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 7 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 8 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 9 from 373 (in) to 388 (out) : -130.573 Transmembrane helix 10 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 3 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 4 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 5 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 6 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 7 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 8 from 373 (in) to 388 (out) : -130.573 Transmembrane helix 9 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 3 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 4 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 5 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 6 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 7 from 296 (in) to 311 (out) : 124.004 Transmembrane helix 8 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 3 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 4 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 5 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 6 from 296 (in) to 311 (out) : 124.004 Transmembrane helix 7 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 3 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 4 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 5 from 296 (in) to 311 (out) : 124.004 Transmembrane helix 6 from 397 (out) to 412 (in) : -104.51 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 3 from 232 (out) to 247 (in) : -13.704 Transmembrane helix 4 from 262 (in) to 277 (out) : 8.292 Transmembrane helix 5 from 295 (out) to 310 (in) : 130.773 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 2 from 232 (out) to 247 (in) : -13.704 Transmembrane helix 3 from 262 (in) to 277 (out) : 8.292 Transmembrane helix 4 from 295 (out) to 310 (in) : 130.773 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 2 from 262 (in) to 277 (out) : 8.292 Transmembrane helix 3 from 295 (out) to 310 (in) : 130.773 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 2 from 295 (out) to 310 (in) : 130.773 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -87.102 Transmembrane helix 2 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 3 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 4 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 5 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 6 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 7 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 8 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 9 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 10 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 11 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 12 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 13 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 14 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 15 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 16 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 17 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 18 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 19 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 20 from 1 (in) to 0 (out) : 4.017 Transmembrane helix 21 from 1 (out) to 0 (in) : -4.841 Transmembrane helix 22 from 1 (in) to 0 (out) : 4.017 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 22 (in) to 37 (out) : -305.746 Transmembrane helix 2 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 3 from 60 (in) to 75 (out) : -203.873 Transmembrane helix 4 from 79 (out) to 94 (in) : -315.089 Transmembrane helix 5 from 98 (in) to 113 (out) : -188.863 Transmembrane helix 6 from 117 (out) to 132 (in) : -128.486 Transmembrane helix 7 from 136 (in) to 151 (out) : -119.103 Transmembrane helix 8 from 155 (out) to 170 (in) : -602.235 Transmembrane helix 9 from 174 (in) to 189 (out) : -783.659 Transmembrane helix 10 from 193 (out) to 208 (in) : -91.794 Transmembrane helix 11 from 212 (in) to 227 (out) : -135.434 Transmembrane helix 12 from 231 (out) to 246 (in) : -15.99 Transmembrane helix 13 from 250 (in) to 265 (out) : -36.63 Transmembrane helix 14 from 269 (out) to 284 (in) : -60.364 Transmembrane helix 15 from 288 (in) to 303 (out) : -31.603 Transmembrane helix 16 from 307 (out) to 322 (in) : -261.386 Transmembrane helix 17 from 326 (in) to 341 (out) : -508.784 Transmembrane helix 18 from 345 (out) to 360 (in) : -238.651 Transmembrane helix 19 from 364 (in) to 379 (out) : -397.233 Transmembrane helix 20 from 383 (out) to 398 (in) : -152.953 Transmembrane helix 21 from 402 (in) to 417 (out) : -149.388 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -212.686 Transmembrane helix 2 from 44 (in) to 59 (out) : -60.948 Transmembrane helix 3 from 63 (out) to 78 (in) : -216.746 Transmembrane helix 4 from 82 (in) to 97 (out) : -292.965 Transmembrane helix 5 from 101 (out) to 116 (in) : -136.978 Transmembrane helix 6 from 120 (in) to 135 (out) : -144.424 Transmembrane helix 7 from 139 (out) to 154 (in) : -203.33 Transmembrane helix 8 from 159 (in) to 174 (out) : -584.716 Transmembrane helix 9 from 191 (out) to 206 (in) : -176.67 Transmembrane helix 10 from 210 (in) to 225 (out) : -118.664 Transmembrane helix 11 from 229 (out) to 244 (in) : -26.16 Transmembrane helix 12 from 248 (in) to 263 (out) : -38.715 Transmembrane helix 13 from 267 (out) to 282 (in) : -23.235 Transmembrane helix 14 from 286 (in) to 301 (out) : -92.901 Transmembrane helix 15 from 305 (out) to 320 (in) : -166.785 Transmembrane helix 16 from 325 (in) to 340 (out) : -514.901 Transmembrane helix 17 from 344 (out) to 359 (in) : -217.932 Transmembrane helix 18 from 364 (in) to 379 (out) : -397.233 Transmembrane helix 19 from 383 (out) to 398 (in) : -152.953 Transmembrane helix 20 from 402 (in) to 417 (out) : -149.388 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -246.519 Transmembrane helix 2 from 43 (out) to 58 (in) : -40.383 Transmembrane helix 3 from 62 (in) to 77 (out) : -204.513 Transmembrane helix 4 from 81 (out) to 96 (in) : -305.392 Transmembrane helix 5 from 100 (in) to 115 (out) : -156.69 Transmembrane helix 6 from 119 (out) to 134 (in) : -138.58 Transmembrane helix 7 from 138 (in) to 153 (out) : -168.386 Transmembrane helix 8 from 191 (out) to 206 (in) : -176.67 Transmembrane helix 9 from 210 (in) to 225 (out) : -118.664 Transmembrane helix 10 from 229 (out) to 244 (in) : -26.16 Transmembrane helix 11 from 248 (in) to 263 (out) : -38.715 Transmembrane helix 12 from 267 (out) to 282 (in) : -23.235 Transmembrane helix 13 from 286 (in) to 301 (out) : -92.901 Transmembrane helix 14 from 305 (out) to 320 (in) : -166.785 Transmembrane helix 15 from 325 (in) to 340 (out) : -514.901 Transmembrane helix 16 from 344 (out) to 359 (in) : -217.932 Transmembrane helix 17 from 364 (in) to 379 (out) : -397.233 Transmembrane helix 18 from 383 (out) to 398 (in) : -152.953 Transmembrane helix 19 from 402 (in) to 417 (out) : -149.388 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -212.686 Transmembrane helix 2 from 44 (in) to 59 (out) : -60.948 Transmembrane helix 3 from 63 (out) to 78 (in) : -216.746 Transmembrane helix 4 from 82 (in) to 97 (out) : -292.965 Transmembrane helix 5 from 101 (out) to 116 (in) : -136.978 Transmembrane helix 6 from 120 (in) to 135 (out) : -144.424 Transmembrane helix 7 from 139 (out) to 154 (in) : -203.33 Transmembrane helix 8 from 198 (in) to 213 (out) : 43.456 Transmembrane helix 9 from 217 (out) to 232 (in) : -125.776 Transmembrane helix 10 from 236 (in) to 251 (out) : -9.665 Transmembrane helix 11 from 255 (out) to 270 (in) : 1.366 Transmembrane helix 12 from 274 (in) to 289 (out) : -150.263 Transmembrane helix 13 from 293 (out) to 308 (in) : 112.156 Transmembrane helix 14 from 312 (in) to 327 (out) : -445.427 Transmembrane helix 15 from 343 (out) to 358 (in) : -211.271 Transmembrane helix 16 from 364 (in) to 379 (out) : -397.233 Transmembrane helix 17 from 383 (out) to 398 (in) : -152.953 Transmembrane helix 18 from 402 (in) to 417 (out) : -149.388 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -246.519 Transmembrane helix 2 from 43 (out) to 58 (in) : -40.383 Transmembrane helix 3 from 62 (in) to 77 (out) : -204.513 Transmembrane helix 4 from 81 (out) to 96 (in) : -305.392 Transmembrane helix 5 from 100 (in) to 115 (out) : -156.69 Transmembrane helix 6 from 119 (out) to 134 (in) : -138.58 Transmembrane helix 7 from 138 (in) to 153 (out) : -168.386 Transmembrane helix 8 from 198 (out) to 213 (in) : 38.344 Transmembrane helix 9 from 217 (in) to 232 (out) : -130.924 Transmembrane helix 10 from 236 (out) to 251 (in) : -15.735 Transmembrane helix 11 from 255 (in) to 270 (out) : -11.866 Transmembrane helix 12 from 274 (out) to 289 (in) : -164.437 Transmembrane helix 13 from 293 (in) to 308 (out) : 102.644 Transmembrane helix 14 from 312 (out) to 327 (in) : -431.873 Transmembrane helix 15 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 16 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 17 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 25 (out) to 40 (in) : -212.686 Transmembrane helix 2 from 44 (in) to 59 (out) : -60.948 Transmembrane helix 3 from 63 (out) to 78 (in) : -216.746 Transmembrane helix 4 from 82 (in) to 97 (out) : -292.965 Transmembrane helix 5 from 101 (out) to 116 (in) : -136.978 Transmembrane helix 6 from 120 (in) to 135 (out) : -144.424 Transmembrane helix 7 from 139 (out) to 154 (in) : -203.33 Transmembrane helix 8 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 9 from 219 (out) to 234 (in) : -108.471 Transmembrane helix 10 from 238 (in) to 253 (out) : -14.568 Transmembrane helix 11 from 257 (out) to 272 (in) : 14.587 Transmembrane helix 12 from 276 (in) to 291 (out) : -181.891 Transmembrane helix 13 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 14 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 15 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 16 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 25 (in) to 40 (out) : -225.514 Transmembrane helix 2 from 44 (out) to 59 (in) : -51.452 Transmembrane helix 3 from 63 (in) to 78 (out) : -211.054 Transmembrane helix 4 from 86 (out) to 101 (in) : -250.809 Transmembrane helix 5 from 110 (in) to 125 (out) : -98.372 Transmembrane helix 6 from 130 (out) to 145 (in) : -82.195 Transmembrane helix 7 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 8 from 219 (out) to 234 (in) : -108.471 Transmembrane helix 9 from 238 (in) to 253 (out) : -14.568 Transmembrane helix 10 from 257 (out) to 272 (in) : 14.587 Transmembrane helix 11 from 276 (in) to 291 (out) : -181.891 Transmembrane helix 12 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 13 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 14 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 15 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 60 (in) to 75 (out) : -203.873 Transmembrane helix 3 from 86 (out) to 101 (in) : -250.809 Transmembrane helix 4 from 110 (in) to 125 (out) : -98.372 Transmembrane helix 5 from 130 (out) to 145 (in) : -82.195 Transmembrane helix 6 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 7 from 219 (out) to 234 (in) : -108.471 Transmembrane helix 8 from 238 (in) to 253 (out) : -14.568 Transmembrane helix 9 from 257 (out) to 272 (in) : 14.587 Transmembrane helix 10 from 276 (in) to 291 (out) : -181.891 Transmembrane helix 11 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 12 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 13 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 14 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 25 (in) to 40 (out) : -225.514 Transmembrane helix 2 from 44 (out) to 59 (in) : -51.452 Transmembrane helix 3 from 63 (in) to 78 (out) : -211.054 Transmembrane helix 4 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 5 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 6 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 7 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 8 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 9 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 10 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 11 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 12 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 13 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 60 (in) to 75 (out) : -203.873 Transmembrane helix 3 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 4 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 5 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 6 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 7 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 8 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 9 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 10 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 11 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 12 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 3 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 4 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 5 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 6 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 7 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 8 from 295 (out) to 310 (in) : 130.773 Transmembrane helix 9 from 343 (in) to 358 (out) : -206.529 Transmembrane helix 10 from 374 (out) to 389 (in) : -131.174 Transmembrane helix 11 from 397 (in) to 412 (out) : -91.59 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 60 (in) to 75 (out) : -203.873 Transmembrane helix 3 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 4 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 5 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 6 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 7 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 8 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 9 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 10 from 373 (in) to 388 (out) : -130.573 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 3 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 4 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 5 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 6 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 7 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 8 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 9 from 373 (in) to 388 (out) : -130.573 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 3 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 4 from 228 (in) to 243 (out) : -30.182 Transmembrane helix 5 from 247 (out) to 262 (in) : -39.221 Transmembrane helix 6 from 266 (in) to 281 (out) : -8.995 Transmembrane helix 7 from 297 (out) to 312 (in) : 122.931 Transmembrane helix 8 from 373 (in) to 388 (out) : -130.573 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 107 (out) to 122 (in) : -92.54 Transmembrane helix 3 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 4 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 5 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 6 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 7 from 296 (in) to 311 (out) : 124.004 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 41 (out) to 56 (in) : -30.405 Transmembrane helix 2 from 131 (in) to 146 (out) : -66.847 Transmembrane helix 3 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 4 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 5 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 6 from 296 (in) to 311 (out) : 124.004 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 41 (in) to 56 (out) : -39.095 Transmembrane helix 2 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 3 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 4 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 5 from 296 (in) to 311 (out) : 124.004 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 2 from 234 (in) to 249 (out) : -6.564 Transmembrane helix 3 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 4 from 296 (in) to 311 (out) : 124.004 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 200 (in) to 215 (out) : 58.243 Transmembrane helix 2 from 261 (out) to 276 (in) : 26.192 Transmembrane helix 3 from 296 (in) to 311 (out) : 124.004 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 200 (out) to 215 (in) : 54.657 Transmembrane helix 2 from 296 (in) to 311 (out) : 124.004 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.124004 1 helix (-) : Score = 0.130773 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 295-310 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: 0.130773