Processing 1 helix: Transmembrane helix 1 from 87 (in) to 111 (out) : 4333.84 Score = 4.333838 Processing 1 helix: Transmembrane helix 1 from 87 (out) to 111 (in) : 4294.96 Score = 4.294964 Processing 21 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -1360.78 Transmembrane helix 2 from 26 (out) to 41 (in) : -284.436 Transmembrane helix 3 from 45 (in) to 60 (out) : 1267.28 Transmembrane helix 4 from 64 (out) to 79 (in) : 611.135 Transmembrane helix 5 from 83 (in) to 98 (out) : 2781.74 Transmembrane helix 6 from 102 (out) to 117 (in) : 873.686 Transmembrane helix 7 from 121 (in) to 136 (out) : -459.466 Transmembrane helix 8 from 140 (out) to 155 (in) : 3479.06 Transmembrane helix 9 from 159 (in) to 174 (out) : -135.051 Transmembrane helix 10 from 178 (out) to 193 (in) : -1034.53 Transmembrane helix 11 from 197 (in) to 212 (out) : 692.847 Transmembrane helix 12 from 216 (out) to 231 (in) : 1480.23 Transmembrane helix 13 from 235 (in) to 250 (out) : 1887.95 Transmembrane helix 14 from 254 (out) to 269 (in) : 955.393 Transmembrane helix 15 from 273 (in) to 288 (out) : -1192.07 Transmembrane helix 16 from 292 (out) to 307 (in) : -1208.79 Transmembrane helix 17 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 18 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 19 from 349 (in) to 364 (out) : 722.296 Transmembrane helix 20 from 368 (out) to 383 (in) : 334.848 Transmembrane helix 21 from 387 (in) to 402 (out) : -1075.12 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 43 (in) to 58 (out) : 1074.22 Transmembrane helix 3 from 62 (out) to 77 (in) : 780.397 Transmembrane helix 4 from 81 (in) to 96 (out) : 2119.71 Transmembrane helix 5 from 100 (out) to 115 (in) : 1389.59 Transmembrane helix 6 from 119 (in) to 134 (out) : -639.382 Transmembrane helix 7 from 138 (out) to 153 (in) : 3568.87 Transmembrane helix 8 from 157 (in) to 172 (out) : 48.112 Transmembrane helix 9 from 176 (out) to 191 (in) : -1025.66 Transmembrane helix 10 from 195 (in) to 210 (out) : 419.196 Transmembrane helix 11 from 214 (out) to 229 (in) : 1772.35 Transmembrane helix 12 from 233 (in) to 248 (out) : 1445.2 Transmembrane helix 13 from 252 (out) to 267 (in) : 1458.76 Transmembrane helix 14 from 271 (in) to 286 (out) : -1152.89 Transmembrane helix 15 from 292 (out) to 307 (in) : -1208.79 Transmembrane helix 16 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 17 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 18 from 349 (in) to 364 (out) : 722.296 Transmembrane helix 19 from 368 (out) to 383 (in) : 334.848 Transmembrane helix 20 from 387 (in) to 402 (out) : -1075.12 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 125 (out) to 140 (in) : 429.641 Transmembrane helix 7 from 144 (in) to 159 (out) : 2710.79 Transmembrane helix 8 from 163 (out) to 178 (in) : -404.949 Transmembrane helix 9 from 185 (in) to 200 (out) : -730.473 Transmembrane helix 10 from 204 (out) to 219 (in) : 1810.81 Transmembrane helix 11 from 223 (in) to 238 (out) : 451.971 Transmembrane helix 12 from 242 (out) to 257 (in) : 3154.46 Transmembrane helix 13 from 261 (in) to 276 (out) : -518.638 Transmembrane helix 14 from 280 (out) to 295 (in) : -1235.76 Transmembrane helix 15 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 16 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 17 from 349 (in) to 364 (out) : 722.296 Transmembrane helix 18 from 368 (out) to 383 (in) : 334.848 Transmembrane helix 19 from 387 (in) to 402 (out) : -1075.12 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 102 (out) : 3716.05 Transmembrane helix 5 from 106 (out) to 121 (in) : 150.483 Transmembrane helix 6 from 125 (in) to 140 (out) : 428.359 Transmembrane helix 7 from 144 (out) to 159 (in) : 2703.21 Transmembrane helix 8 from 163 (in) to 178 (out) : -401.551 Transmembrane helix 9 from 185 (out) to 200 (in) : -717.827 Transmembrane helix 10 from 204 (in) to 219 (out) : 1832.79 Transmembrane helix 11 from 223 (out) to 238 (in) : 447.629 Transmembrane helix 12 from 242 (in) to 257 (out) : 3145.94 Transmembrane helix 13 from 261 (out) to 276 (in) : -521.262 Transmembrane helix 14 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 15 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 16 from 349 (in) to 364 (out) : 722.296 Transmembrane helix 17 from 368 (out) to 383 (in) : 334.848 Transmembrane helix 18 from 387 (in) to 402 (out) : -1075.12 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 136 (out) to 154 (in) : 3792 Transmembrane helix 7 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 8 from 185 (out) to 200 (in) : -717.827 Transmembrane helix 9 from 204 (in) to 219 (out) : 1832.79 Transmembrane helix 10 from 223 (out) to 238 (in) : 447.629 Transmembrane helix 11 from 242 (in) to 257 (out) : 3145.94 Transmembrane helix 12 from 261 (out) to 276 (in) : -521.262 Transmembrane helix 13 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 14 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 15 from 349 (in) to 364 (out) : 722.296 Transmembrane helix 16 from 368 (out) to 383 (in) : 334.848 Transmembrane helix 17 from 387 (in) to 402 (out) : -1075.12 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 103 (out) : 3851.15 Transmembrane helix 5 from 107 (out) to 122 (in) : 18.303 Transmembrane helix 6 from 136 (in) to 154 (out) : 3800.4 Transmembrane helix 7 from 158 (out) to 173 (in) : -55.545 Transmembrane helix 8 from 185 (in) to 200 (out) : -730.473 Transmembrane helix 9 from 204 (out) to 219 (in) : 1810.81 Transmembrane helix 10 from 223 (in) to 238 (out) : 451.971 Transmembrane helix 11 from 242 (out) to 257 (in) : 3154.46 Transmembrane helix 12 from 261 (in) to 276 (out) : -518.638 Transmembrane helix 13 from 310 (out) to 325 (in) : 594.023 Transmembrane helix 14 from 329 (in) to 344 (out) : 2116.29 Transmembrane helix 15 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 16 from 369 (in) to 384 (out) : 248.034 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 136 (out) to 154 (in) : 3792 Transmembrane helix 7 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 8 from 195 (out) to 210 (in) : 416.104 Transmembrane helix 9 from 214 (in) to 229 (out) : 1780.35 Transmembrane helix 10 from 233 (out) to 248 (in) : 1464 Transmembrane helix 11 from 252 (in) to 267 (out) : 1440.34 Transmembrane helix 12 from 310 (out) to 325 (in) : 594.023 Transmembrane helix 13 from 329 (in) to 344 (out) : 2116.29 Transmembrane helix 14 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 15 from 369 (in) to 384 (out) : 248.034 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 103 (out) : 3851.15 Transmembrane helix 5 from 107 (out) to 122 (in) : 18.303 Transmembrane helix 6 from 136 (in) to 154 (out) : 3800.4 Transmembrane helix 7 from 158 (out) to 173 (in) : -55.545 Transmembrane helix 8 from 204 (in) to 219 (out) : 1832.79 Transmembrane helix 9 from 223 (out) to 238 (in) : 447.629 Transmembrane helix 10 from 242 (in) to 262 (out) : 3421.09 Transmembrane helix 11 from 310 (out) to 325 (in) : 594.023 Transmembrane helix 12 from 329 (in) to 344 (out) : 2116.29 Transmembrane helix 13 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 14 from 369 (in) to 384 (out) : 248.034 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 136 (out) to 154 (in) : 3792 Transmembrane helix 7 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 8 from 203 (out) to 218 (in) : 1674.71 Transmembrane helix 9 from 222 (in) to 237 (out) : 568.088 Transmembrane helix 10 from 241 (out) to 262 (in) : 3458.91 Transmembrane helix 11 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 12 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 13 from 369 (in) to 384 (out) : 248.034 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 5 from 136 (out) to 154 (in) : 3792 Transmembrane helix 6 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 7 from 203 (out) to 218 (in) : 1674.71 Transmembrane helix 8 from 222 (in) to 237 (out) : 568.088 Transmembrane helix 9 from 241 (out) to 262 (in) : 3458.91 Transmembrane helix 10 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 11 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 12 from 369 (in) to 384 (out) : 248.034 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 5 from 136 (in) to 154 (out) : 3800.4 Transmembrane helix 6 from 158 (out) to 173 (in) : -55.545 Transmembrane helix 7 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 8 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 9 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 10 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 11 from 369 (in) to 384 (out) : 248.034 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 5 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 6 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 7 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 8 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 9 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 10 from 369 (in) to 384 (out) : 248.034 Score = 21.559523 Processing 9 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 45 (out) to 75 (in) : 2072.11 Transmembrane helix 3 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 4 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 5 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 6 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 7 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 8 from 350 (out) to 365 (in) : 800.73 Transmembrane helix 9 from 369 (in) to 384 (out) : 248.034 Score = 21.786285 Processing 8 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 45 (in) to 75 (out) : 2048.09 Transmembrane helix 3 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 4 from 136 (in) to 163 (out) : 4067.42 Transmembrane helix 5 from 204 (out) to 228 (in) : 2540.88 Transmembrane helix 6 from 237 (in) to 262 (out) : 3442.21 Transmembrane helix 7 from 316 (out) to 343 (in) : 3103.16 Transmembrane helix 8 from 353 (in) to 376 (out) : 1275.93 Score = 21.601933 Processing 7 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 45 (out) to 75 (in) : 2072.11 Transmembrane helix 3 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 4 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 5 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 6 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 7 from 316 (in) to 343 (out) : 3142.64 Score = 20.753122 Processing 6 helices: Transmembrane helix 1 from 45 (out) to 75 (in) : 2072.11 Transmembrane helix 2 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 3 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 4 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 5 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 6 from 316 (in) to 343 (out) : 3142.64 Score = 19.815945 Processing 5 helices: Transmembrane helix 1 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 2 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 3 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 4 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 5 from 316 (in) to 343 (out) : 3142.64 Score = 17.666265 Processing 4 helices: Transmembrane helix 1 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 2 from 136 (in) to 163 (out) : 4067.42 Transmembrane helix 3 from 237 (out) to 262 (in) : 3474.79 Transmembrane helix 4 from 316 (in) to 343 (out) : 3142.64 Score = 15.000503 Processing 3 helices: Transmembrane helix 1 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 2 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 3 from 240 (in) to 262 (out) : 3448.03 Score = 11.873721 Processing 2 helices: Transmembrane helix 1 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 2 from 136 (in) to 163 (out) : 4067.42 Score = 8.327512 Processing 21 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -1376.22 Transmembrane helix 2 from 26 (in) to 41 (out) : -288.764 Transmembrane helix 3 from 45 (out) to 60 (in) : 1278.12 Transmembrane helix 4 from 64 (in) to 79 (out) : 585.365 Transmembrane helix 5 from 83 (out) to 98 (in) : 2768.46 Transmembrane helix 6 from 102 (in) to 117 (out) : 877.114 Transmembrane helix 7 from 121 (out) to 136 (in) : -454.234 Transmembrane helix 8 from 140 (in) to 155 (out) : 3490.34 Transmembrane helix 9 from 159 (out) to 174 (in) : -143.149 Transmembrane helix 10 from 178 (in) to 193 (out) : -1050.37 Transmembrane helix 11 from 197 (out) to 212 (in) : 684.453 Transmembrane helix 12 from 216 (in) to 231 (out) : 1490.57 Transmembrane helix 13 from 235 (out) to 250 (in) : 1909.75 Transmembrane helix 14 from 254 (in) to 269 (out) : 940.507 Transmembrane helix 15 from 273 (out) to 288 (in) : -1186.33 Transmembrane helix 16 from 292 (in) to 307 (out) : -1211.11 Transmembrane helix 17 from 311 (out) to 326 (in) : 766.075 Transmembrane helix 18 from 330 (in) to 345 (out) : 1943.5 Transmembrane helix 19 from 349 (out) to 364 (in) : 716.604 Transmembrane helix 20 from 368 (in) to 383 (out) : 319.652 Transmembrane helix 21 from 387 (out) to 402 (in) : -1073.78 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 34 (out) to 49 (in) : -169.884 Transmembrane helix 3 from 53 (in) to 68 (out) : 1454.95 Transmembrane helix 4 from 72 (out) to 87 (in) : -23.911 Transmembrane helix 5 from 91 (in) to 106 (out) : 3671.16 Transmembrane helix 6 from 110 (out) to 125 (in) : -342.482 Transmembrane helix 7 from 129 (in) to 144 (out) : 1639.91 Transmembrane helix 8 from 148 (out) to 163 (in) : 1657.71 Transmembrane helix 9 from 167 (in) to 182 (out) : -681.758 Transmembrane helix 10 from 186 (out) to 201 (in) : -661.369 Transmembrane helix 11 from 205 (in) to 220 (out) : 1912.91 Transmembrane helix 12 from 224 (out) to 239 (in) : 331.82 Transmembrane helix 13 from 243 (in) to 258 (out) : 3166.85 Transmembrane helix 14 from 262 (out) to 277 (in) : -667.688 Transmembrane helix 15 from 292 (in) to 307 (out) : -1211.11 Transmembrane helix 16 from 311 (out) to 326 (in) : 766.075 Transmembrane helix 17 from 330 (in) to 345 (out) : 1943.5 Transmembrane helix 18 from 349 (out) to 364 (in) : 716.604 Transmembrane helix 19 from 368 (in) to 383 (out) : 319.652 Transmembrane helix 20 from 387 (out) to 402 (in) : -1073.78 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 102 (out) : 3716.05 Transmembrane helix 5 from 106 (out) to 121 (in) : 150.483 Transmembrane helix 6 from 125 (in) to 140 (out) : 428.359 Transmembrane helix 7 from 144 (out) to 159 (in) : 2703.21 Transmembrane helix 8 from 163 (in) to 178 (out) : -401.551 Transmembrane helix 9 from 185 (out) to 200 (in) : -717.827 Transmembrane helix 10 from 204 (in) to 219 (out) : 1832.79 Transmembrane helix 11 from 223 (out) to 238 (in) : 447.629 Transmembrane helix 12 from 242 (in) to 257 (out) : 3145.94 Transmembrane helix 13 from 261 (out) to 276 (in) : -521.262 Transmembrane helix 14 from 280 (in) to 295 (out) : -1244.64 Transmembrane helix 15 from 304 (out) to 319 (in) : -316.339 Transmembrane helix 16 from 323 (in) to 338 (out) : 2549.87 Transmembrane helix 17 from 342 (out) to 357 (in) : 199.284 Transmembrane helix 18 from 361 (in) to 376 (out) : 885.699 Transmembrane helix 19 from 380 (out) to 395 (in) : -602.389 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 125 (out) to 140 (in) : 429.641 Transmembrane helix 7 from 144 (in) to 159 (out) : 2710.79 Transmembrane helix 8 from 163 (out) to 178 (in) : -404.949 Transmembrane helix 9 from 185 (in) to 200 (out) : -730.473 Transmembrane helix 10 from 204 (out) to 219 (in) : 1810.81 Transmembrane helix 11 from 223 (in) to 238 (out) : 451.971 Transmembrane helix 12 from 242 (out) to 257 (in) : 3154.46 Transmembrane helix 13 from 261 (in) to 276 (out) : -518.638 Transmembrane helix 14 from 304 (out) to 319 (in) : -316.339 Transmembrane helix 15 from 323 (in) to 338 (out) : 2549.87 Transmembrane helix 16 from 342 (out) to 357 (in) : 199.284 Transmembrane helix 17 from 361 (in) to 376 (out) : 885.699 Transmembrane helix 18 from 380 (out) to 395 (in) : -602.389 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 102 (out) : 3716.05 Transmembrane helix 5 from 106 (out) to 121 (in) : 150.483 Transmembrane helix 6 from 125 (in) to 140 (out) : 428.359 Transmembrane helix 7 from 144 (out) to 159 (in) : 2703.21 Transmembrane helix 8 from 163 (in) to 178 (out) : -401.551 Transmembrane helix 9 from 185 (out) to 200 (in) : -717.827 Transmembrane helix 10 from 204 (in) to 219 (out) : 1832.79 Transmembrane helix 11 from 223 (out) to 238 (in) : 447.629 Transmembrane helix 12 from 242 (in) to 257 (out) : 3145.94 Transmembrane helix 13 from 261 (out) to 276 (in) : -521.262 Transmembrane helix 14 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 15 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 16 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 17 from 369 (out) to 384 (in) : 264.766 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 136 (out) to 154 (in) : 3792 Transmembrane helix 7 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 8 from 185 (out) to 200 (in) : -717.827 Transmembrane helix 9 from 204 (in) to 219 (out) : 1832.79 Transmembrane helix 10 from 223 (out) to 238 (in) : 447.629 Transmembrane helix 11 from 242 (in) to 257 (out) : 3145.94 Transmembrane helix 12 from 261 (out) to 276 (in) : -521.262 Transmembrane helix 13 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 14 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 15 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 16 from 369 (out) to 384 (in) : 264.766 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 103 (out) : 3851.15 Transmembrane helix 5 from 107 (out) to 122 (in) : 18.303 Transmembrane helix 6 from 136 (in) to 154 (out) : 3800.4 Transmembrane helix 7 from 158 (out) to 173 (in) : -55.545 Transmembrane helix 8 from 195 (in) to 210 (out) : 419.196 Transmembrane helix 9 from 214 (out) to 229 (in) : 1772.35 Transmembrane helix 10 from 233 (in) to 248 (out) : 1445.2 Transmembrane helix 11 from 252 (out) to 267 (in) : 1458.76 Transmembrane helix 12 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 13 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 14 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 15 from 369 (out) to 384 (in) : 264.766 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 102 (in) : 3696.15 Transmembrane helix 5 from 106 (in) to 121 (out) : 149.117 Transmembrane helix 6 from 136 (out) to 154 (in) : 3792 Transmembrane helix 7 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 8 from 203 (out) to 218 (in) : 1674.71 Transmembrane helix 9 from 222 (in) to 237 (out) : 568.088 Transmembrane helix 10 from 241 (out) to 262 (in) : 3458.91 Transmembrane helix 11 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 12 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 13 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 14 from 369 (out) to 384 (in) : 264.766 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 5 from 136 (out) to 154 (in) : 3792 Transmembrane helix 6 from 158 (in) to 173 (out) : -48.755 Transmembrane helix 7 from 203 (out) to 218 (in) : 1674.71 Transmembrane helix 8 from 222 (in) to 237 (out) : 568.088 Transmembrane helix 9 from 241 (out) to 262 (in) : 3458.91 Transmembrane helix 10 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 11 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 12 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 13 from 369 (out) to 384 (in) : 264.766 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 44 (out) to 59 (in) : 1182.4 Transmembrane helix 3 from 63 (in) to 78 (out) : 687.499 Transmembrane helix 4 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 5 from 136 (in) to 154 (out) : 3800.4 Transmembrane helix 6 from 158 (out) to 173 (in) : -55.545 Transmembrane helix 7 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 8 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 9 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 10 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 11 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 12 from 369 (out) to 384 (in) : 264.766 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 5 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 6 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 7 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 8 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 9 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 10 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 11 from 369 (out) to 384 (in) : 264.766 Score = 21.201565 Processing 10 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 45 (out) to 75 (in) : 2072.11 Transmembrane helix 3 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 4 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 5 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 6 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 7 from 311 (in) to 326 (out) : 785.125 Transmembrane helix 8 from 330 (out) to 345 (in) : 1943.2 Transmembrane helix 9 from 350 (in) to 365 (out) : 806.57 Transmembrane helix 10 from 369 (out) to 384 (in) : 264.766 Score = 21.428326 Processing 9 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 977.795 Transmembrane helix 2 from 44 (in) to 59 (out) : 1170.5 Transmembrane helix 3 from 63 (out) to 78 (in) : 712.701 Transmembrane helix 4 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 5 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 6 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 7 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 8 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 9 from 355 (out) to 377 (in) : 1275.41 Score = 21.834314 Processing 8 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 981.305 Transmembrane helix 2 from 45 (out) to 75 (in) : 2072.11 Transmembrane helix 3 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 4 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 5 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 6 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 7 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 8 from 355 (out) to 377 (in) : 1275.41 Score = 22.061077 Processing 7 helices: Transmembrane helix 1 from 45 (out) to 75 (in) : 2072.11 Transmembrane helix 2 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 3 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 4 from 204 (in) to 227 (out) : 2569.15 Transmembrane helix 5 from 240 (out) to 262 (in) : 3471.57 Transmembrane helix 6 from 316 (in) to 343 (out) : 3142.64 Transmembrane helix 7 from 355 (out) to 377 (in) : 1275.41 Score = 21.123898 Processing 6 helices: Transmembrane helix 1 from 45 (in) to 75 (out) : 2048.09 Transmembrane helix 2 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 3 from 136 (in) to 163 (out) : 4067.42 Transmembrane helix 4 from 204 (out) to 228 (in) : 2540.88 Transmembrane helix 5 from 237 (in) to 262 (out) : 3442.21 Transmembrane helix 6 from 316 (out) to 343 (in) : 3103.16 Score = 19.335831 Processing 5 helices: Transmembrane helix 1 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 2 from 136 (in) to 163 (out) : 4067.42 Transmembrane helix 3 from 204 (out) to 228 (in) : 2540.88 Transmembrane helix 4 from 237 (in) to 262 (out) : 3442.21 Transmembrane helix 5 from 316 (out) to 343 (in) : 3103.16 Score = 17.365309 Processing 4 helices: Transmembrane helix 1 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 2 from 136 (out) to 163 (in) : 4056.99 Transmembrane helix 3 from 240 (in) to 262 (out) : 3448.03 Transmembrane helix 4 from 316 (out) to 343 (in) : 3103.16 Score = 14.921320 Processing 3 helices: Transmembrane helix 1 from 87 (out) to 111 (in) : 4294.96 Transmembrane helix 2 from 136 (in) to 163 (out) : 4067.42 Transmembrane helix 3 from 237 (out) to 262 (in) : 3474.79 Score = 11.802303 Processing 2 helices: Transmembrane helix 1 from 87 (in) to 111 (out) : 4333.84 Transmembrane helix 2 from 136 (out) to 163 (in) : 4056.99 Score = 8.425691 Summary of topology analysis: 1 helix (+) : Score = 4.33384 1 helix (-) : Score = 4.29496 2 helices (+) : Score = 8.42569 2 helices (-) : Score = 8.32751 3 helices (+) : Score = 11.8737 3 helices (-) : Score = 11.8023 4 helices (+) : Score = 14.9213 4 helices (-) : Score = 15.0005 5 helices (+) : Score = 17.6663 5 helices (-) : Score = 17.3653 6 helices (+) : Score = 19.3358 6 helices (-) : Score = 19.8159 7 helices (+) : Score = 20.7531 7 helices (-) : Score = 21.1239 8 helices (+) : Score = 22.0611 8 helices (-) : Score = 21.6019 9 helices (+) : Score = 21.7863 9 helices (-) : Score = 21.8343 10 helices (+) : Score = 21.4283 10 helices (-) : Score = 21.5595 11 helices (+) : Score = -100000 11 helices (-) : Score = 21.2016 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 5.964 Topology: 15-30,45-75,87-111,136-163,204-227,240-262,316-343,355-377 Re-entrant helices: Not detected. Helix count: 8 N-terminal: in Score: 22.0611