Processing 1 helix: Transmembrane helix 1 from 292 (in) to 322 (out) : 4363.7 Score = 4.363699 Processing 1 helix: Transmembrane helix 1 from 293 (out) to 323 (in) : 4301.94 Score = 4.301938 Processing 21 helices: Transmembrane helix 1 from 7 (in) to 22 (out) : -277.719 Transmembrane helix 2 from 26 (out) to 41 (in) : 785.687 Transmembrane helix 3 from 45 (in) to 60 (out) : 276.479 Transmembrane helix 4 from 64 (out) to 79 (in) : 297.236 Transmembrane helix 5 from 83 (in) to 98 (out) : 1174.19 Transmembrane helix 6 from 102 (out) to 117 (in) : 988.206 Transmembrane helix 7 from 121 (in) to 136 (out) : 179.711 Transmembrane helix 8 from 140 (out) to 155 (in) : -309.607 Transmembrane helix 9 from 159 (in) to 174 (out) : 813.362 Transmembrane helix 10 from 178 (out) to 193 (in) : 1797.14 Transmembrane helix 11 from 197 (in) to 212 (out) : -1044.08 Transmembrane helix 12 from 216 (out) to 231 (in) : 437.742 Transmembrane helix 13 from 235 (in) to 250 (out) : 1921.37 Transmembrane helix 14 from 254 (out) to 269 (in) : 876.163 Transmembrane helix 15 from 273 (in) to 288 (out) : 24.9511 Transmembrane helix 16 from 292 (out) to 307 (in) : 2582.69 Transmembrane helix 17 from 311 (in) to 326 (out) : 2143.76 Transmembrane helix 18 from 330 (out) to 345 (in) : -97.385 Transmembrane helix 19 from 349 (in) to 364 (out) : 512.969 Transmembrane helix 20 from 368 (out) to 383 (in) : 1332.68 Transmembrane helix 21 from 387 (in) to 402 (out) : 2098 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 51 (out) to 66 (in) : 611.855 Transmembrane helix 4 from 70 (in) to 85 (out) : 16.87 Transmembrane helix 5 from 89 (out) to 104 (in) : 1447.76 Transmembrane helix 6 from 108 (in) to 123 (out) : 1073.32 Transmembrane helix 7 from 127 (out) to 142 (in) : -170.696 Transmembrane helix 8 from 146 (in) to 161 (out) : 34.172 Transmembrane helix 9 from 165 (out) to 180 (in) : 910.145 Transmembrane helix 10 from 184 (in) to 199 (out) : 1106.63 Transmembrane helix 11 from 208 (out) to 223 (in) : -535.212 Transmembrane helix 12 from 227 (in) to 243 (out) : 2491.47 Transmembrane helix 13 from 247 (out) to 262 (in) : 269.691 Transmembrane helix 14 from 266 (in) to 281 (out) : 1099.54 Transmembrane helix 15 from 289 (out) to 306 (in) : 2696.13 Transmembrane helix 16 from 310 (in) to 325 (out) : 2045.67 Transmembrane helix 17 from 329 (out) to 344 (in) : 1.932 Transmembrane helix 18 from 348 (in) to 363 (out) : 477.693 Transmembrane helix 19 from 367 (out) to 382 (in) : 1220.95 Transmembrane helix 20 from 386 (in) to 401 (out) : 2353.37 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : 221.303 Transmembrane helix 2 from 32 (out) to 47 (in) : 596.877 Transmembrane helix 3 from 51 (in) to 66 (out) : 602.345 Transmembrane helix 4 from 70 (out) to 85 (in) : 38.33 Transmembrane helix 5 from 89 (in) to 104 (out) : 1479.84 Transmembrane helix 6 from 108 (out) to 123 (in) : 1091.38 Transmembrane helix 7 from 127 (in) to 142 (out) : -184.804 Transmembrane helix 8 from 146 (out) to 161 (in) : 30.228 Transmembrane helix 9 from 165 (in) to 180 (out) : 927.455 Transmembrane helix 10 from 184 (out) to 199 (in) : 1139.46 Transmembrane helix 11 from 208 (in) to 223 (out) : -533.088 Transmembrane helix 12 from 227 (out) to 242 (in) : 2350.24 Transmembrane helix 13 from 246 (in) to 261 (out) : 250.035 Transmembrane helix 14 from 265 (out) to 280 (in) : 1249.72 Transmembrane helix 15 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 16 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 17 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 18 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 19 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 68 (in) : 741.611 Transmembrane helix 4 from 72 (in) to 87 (out) : 66.86 Transmembrane helix 5 from 91 (out) to 106 (in) : 1323.86 Transmembrane helix 6 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 7 from 139 (out) to 154 (in) : -324.838 Transmembrane helix 8 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 9 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 10 from 208 (in) to 223 (out) : -533.088 Transmembrane helix 11 from 227 (out) to 242 (in) : 2350.24 Transmembrane helix 12 from 246 (in) to 261 (out) : 250.035 Transmembrane helix 13 from 265 (out) to 280 (in) : 1249.72 Transmembrane helix 14 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 15 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 16 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 17 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 18 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : 221.303 Transmembrane helix 2 from 32 (out) to 47 (in) : 596.877 Transmembrane helix 3 from 51 (in) to 66 (out) : 602.345 Transmembrane helix 4 from 70 (out) to 85 (in) : 38.33 Transmembrane helix 5 from 89 (in) to 104 (out) : 1479.84 Transmembrane helix 6 from 108 (out) to 123 (in) : 1091.38 Transmembrane helix 7 from 127 (in) to 142 (out) : -184.804 Transmembrane helix 8 from 146 (out) to 161 (in) : 30.228 Transmembrane helix 9 from 165 (in) to 180 (out) : 927.455 Transmembrane helix 10 from 184 (out) to 199 (in) : 1139.46 Transmembrane helix 11 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 12 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 13 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 14 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 15 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 16 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 17 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 68 (in) : 741.611 Transmembrane helix 4 from 72 (in) to 87 (out) : 66.86 Transmembrane helix 5 from 91 (out) to 106 (in) : 1323.86 Transmembrane helix 6 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 7 from 139 (out) to 154 (in) : -324.838 Transmembrane helix 8 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 9 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 10 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 11 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 12 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 13 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 14 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 15 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 16 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 368.117 Transmembrane helix 2 from 34 (out) to 49 (in) : 457.255 Transmembrane helix 3 from 53 (in) to 68 (out) : 722.989 Transmembrane helix 4 from 72 (out) to 87 (in) : 72.14 Transmembrane helix 5 from 91 (in) to 106 (out) : 1353.94 Transmembrane helix 6 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 7 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 8 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 9 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 10 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 11 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 12 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 13 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 14 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 15 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 4 from 84 (in) to 104 (out) : 1615.73 Transmembrane helix 5 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 6 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 7 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 8 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 9 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 10 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 11 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 12 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 13 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 14 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 20 (in) to 44 (out) : 1151.63 Transmembrane helix 2 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 3 from 84 (in) to 104 (out) : 1615.73 Transmembrane helix 4 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 5 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 6 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 7 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 8 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 9 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 10 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 11 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 12 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 13 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 20 (out) to 45 (in) : 1104.74 Transmembrane helix 2 from 53 (in) to 74 (out) : 820.253 Transmembrane helix 3 from 84 (out) to 104 (in) : 1570.07 Transmembrane helix 4 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 5 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 6 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 7 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 8 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 9 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 10 from 339 (in) to 354 (out) : 23.805 Transmembrane helix 11 from 358 (out) to 373 (in) : 675.087 Transmembrane helix 12 from 377 (in) to 397 (out) : 3602.58 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 20 (in) to 44 (out) : 1151.63 Transmembrane helix 2 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 3 from 84 (in) to 104 (out) : 1615.73 Transmembrane helix 4 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 5 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 6 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 7 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 8 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 9 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 10 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 11 from 366 (in) to 396 (out) : 4023.24 Score = 21.493031 Processing 10 helices: Transmembrane helix 1 from 20 (out) to 45 (in) : 1104.74 Transmembrane helix 2 from 53 (in) to 74 (out) : 820.253 Transmembrane helix 3 from 84 (out) to 104 (in) : 1570.07 Transmembrane helix 4 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 5 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 6 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 7 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 8 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 9 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 10 from 366 (in) to 396 (out) : 4023.24 Score = 21.044302 Processing 9 helices: Transmembrane helix 1 from 20 (in) to 44 (out) : 1151.63 Transmembrane helix 2 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 3 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 4 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 5 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 6 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 7 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 8 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 9 from 366 (in) to 396 (out) : 4023.24 Score = 20.890034 Processing 8 helices: Transmembrane helix 1 from 20 (out) to 45 (in) : 1104.74 Transmembrane helix 2 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 3 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 4 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 5 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 6 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 7 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 8 from 366 (in) to 396 (out) : 4023.24 Score = 19.848276 Processing 7 helices: Transmembrane helix 1 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 2 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 3 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 4 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 5 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 6 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 7 from 366 (in) to 396 (out) : 4023.24 Score = 18.821802 Processing 6 helices: Transmembrane helix 1 from 91 (out) to 121 (in) : 2352.93 Transmembrane helix 2 from 163 (in) to 193 (out) : 2466.44 Transmembrane helix 3 from 226 (out) to 249 (in) : 2826.85 Transmembrane helix 4 from 260 (in) to 276 (out) : 1533.66 Transmembrane helix 5 from 293 (out) to 323 (in) : 4301.94 Transmembrane helix 6 from 366 (in) to 396 (out) : 4023.24 Score = 17.234606 Processing 5 helices: Transmembrane helix 1 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 2 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 3 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 4 from 293 (out) to 323 (in) : 4301.94 Transmembrane helix 5 from 366 (in) to 396 (out) : 4023.24 Score = 16.103275 Processing 4 helices: Transmembrane helix 1 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 2 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 3 from 293 (out) to 323 (in) : 4301.94 Transmembrane helix 4 from 366 (in) to 396 (out) : 4023.24 Score = 13.697668 Processing 3 helices: Transmembrane helix 1 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 2 from 293 (out) to 323 (in) : 4301.94 Transmembrane helix 3 from 366 (in) to 396 (out) : 4023.24 Score = 11.074715 Processing 2 helices: Transmembrane helix 1 from 293 (out) to 323 (in) : 4301.94 Transmembrane helix 2 from 366 (in) to 396 (out) : 4023.24 Score = 8.247215 Processing 21 helices: Transmembrane helix 1 from 7 (out) to 22 (in) : -301.381 Transmembrane helix 2 from 26 (in) to 41 (out) : 809.913 Transmembrane helix 3 from 45 (out) to 60 (in) : 276.021 Transmembrane helix 4 from 64 (in) to 79 (out) : 270.664 Transmembrane helix 5 from 83 (out) to 98 (in) : 1149.91 Transmembrane helix 6 from 102 (in) to 117 (out) : 981.294 Transmembrane helix 7 from 121 (out) to 136 (in) : 184.689 Transmembrane helix 8 from 140 (in) to 155 (out) : -304.793 Transmembrane helix 9 from 159 (out) to 174 (in) : 809.038 Transmembrane helix 10 from 178 (in) to 193 (out) : 1735.66 Transmembrane helix 11 from 197 (out) to 212 (in) : -1057.02 Transmembrane helix 12 from 216 (in) to 231 (out) : 465.758 Transmembrane helix 13 from 235 (out) to 250 (in) : 1901.53 Transmembrane helix 14 from 254 (in) to 269 (out) : 841.937 Transmembrane helix 15 from 273 (out) to 288 (in) : 30.7491 Transmembrane helix 16 from 292 (in) to 307 (out) : 2624.51 Transmembrane helix 17 from 311 (out) to 326 (in) : 2165.04 Transmembrane helix 18 from 330 (in) to 345 (out) : -108.715 Transmembrane helix 19 from 349 (out) to 364 (in) : 506.031 Transmembrane helix 20 from 368 (in) to 383 (out) : 1334.82 Transmembrane helix 21 from 387 (out) to 402 (in) : 2107.1 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : 221.303 Transmembrane helix 2 from 32 (out) to 47 (in) : 596.877 Transmembrane helix 3 from 51 (in) to 66 (out) : 602.345 Transmembrane helix 4 from 70 (out) to 85 (in) : 38.33 Transmembrane helix 5 from 89 (in) to 104 (out) : 1479.84 Transmembrane helix 6 from 108 (out) to 123 (in) : 1091.38 Transmembrane helix 7 from 127 (in) to 142 (out) : -184.804 Transmembrane helix 8 from 146 (out) to 161 (in) : 30.228 Transmembrane helix 9 from 165 (in) to 180 (out) : 927.455 Transmembrane helix 10 from 184 (out) to 199 (in) : 1139.46 Transmembrane helix 11 from 208 (in) to 223 (out) : -533.088 Transmembrane helix 12 from 227 (out) to 242 (in) : 2350.24 Transmembrane helix 13 from 246 (in) to 261 (out) : 250.035 Transmembrane helix 14 from 265 (out) to 280 (in) : 1249.72 Transmembrane helix 15 from 289 (in) to 306 (out) : 2741.87 Transmembrane helix 16 from 310 (out) to 325 (in) : 2063.33 Transmembrane helix 17 from 329 (in) to 344 (out) : -10.632 Transmembrane helix 18 from 348 (out) to 363 (in) : 469.707 Transmembrane helix 19 from 367 (in) to 382 (out) : 1224.55 Transmembrane helix 20 from 386 (out) to 401 (in) : 2366.23 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 68 (in) : 741.611 Transmembrane helix 4 from 72 (in) to 87 (out) : 66.86 Transmembrane helix 5 from 91 (out) to 106 (in) : 1323.86 Transmembrane helix 6 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 7 from 139 (out) to 154 (in) : -324.838 Transmembrane helix 8 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 9 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 10 from 208 (in) to 223 (out) : -533.088 Transmembrane helix 11 from 227 (out) to 242 (in) : 2350.24 Transmembrane helix 12 from 246 (in) to 261 (out) : 250.035 Transmembrane helix 13 from 265 (out) to 280 (in) : 1249.72 Transmembrane helix 14 from 289 (in) to 306 (out) : 2741.87 Transmembrane helix 15 from 310 (out) to 325 (in) : 2063.33 Transmembrane helix 16 from 329 (in) to 344 (out) : -10.632 Transmembrane helix 17 from 348 (out) to 363 (in) : 469.707 Transmembrane helix 18 from 367 (in) to 382 (out) : 1224.55 Transmembrane helix 19 from 386 (out) to 401 (in) : 2366.23 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : 221.303 Transmembrane helix 2 from 32 (out) to 47 (in) : 596.877 Transmembrane helix 3 from 51 (in) to 66 (out) : 602.345 Transmembrane helix 4 from 70 (out) to 85 (in) : 38.33 Transmembrane helix 5 from 89 (in) to 104 (out) : 1479.84 Transmembrane helix 6 from 108 (out) to 123 (in) : 1091.38 Transmembrane helix 7 from 127 (in) to 142 (out) : -184.804 Transmembrane helix 8 from 146 (out) to 161 (in) : 30.228 Transmembrane helix 9 from 165 (in) to 180 (out) : 927.455 Transmembrane helix 10 from 184 (out) to 199 (in) : 1139.46 Transmembrane helix 11 from 225 (in) to 240 (out) : 2160.62 Transmembrane helix 12 from 244 (out) to 259 (in) : 368.753 Transmembrane helix 13 from 263 (in) to 278 (out) : 1405.14 Transmembrane helix 14 from 289 (out) to 307 (in) : 2749.23 Transmembrane helix 15 from 311 (in) to 332 (out) : 2477.21 Transmembrane helix 16 from 339 (out) to 354 (in) : -0.204981 Transmembrane helix 17 from 358 (in) to 373 (out) : 675.413 Transmembrane helix 18 from 377 (out) to 397 (in) : 3660.22 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 68 (in) : 741.611 Transmembrane helix 4 from 72 (in) to 87 (out) : 66.86 Transmembrane helix 5 from 91 (out) to 106 (in) : 1323.86 Transmembrane helix 6 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 7 from 139 (out) to 154 (in) : -324.838 Transmembrane helix 8 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 9 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 10 from 225 (in) to 240 (out) : 2160.62 Transmembrane helix 11 from 244 (out) to 259 (in) : 368.753 Transmembrane helix 12 from 263 (in) to 278 (out) : 1405.14 Transmembrane helix 13 from 289 (out) to 307 (in) : 2749.23 Transmembrane helix 14 from 311 (in) to 332 (out) : 2477.21 Transmembrane helix 15 from 339 (out) to 354 (in) : -0.204981 Transmembrane helix 16 from 358 (in) to 373 (out) : 675.413 Transmembrane helix 17 from 377 (out) to 397 (in) : 3660.22 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : 221.303 Transmembrane helix 2 from 32 (out) to 47 (in) : 596.877 Transmembrane helix 3 from 51 (in) to 66 (out) : 602.345 Transmembrane helix 4 from 70 (out) to 85 (in) : 38.33 Transmembrane helix 5 from 89 (in) to 104 (out) : 1479.84 Transmembrane helix 6 from 108 (out) to 123 (in) : 1091.38 Transmembrane helix 7 from 127 (in) to 142 (out) : -184.804 Transmembrane helix 8 from 146 (out) to 161 (in) : 30.228 Transmembrane helix 9 from 165 (in) to 180 (out) : 927.455 Transmembrane helix 10 from 184 (out) to 199 (in) : 1139.46 Transmembrane helix 11 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 12 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 13 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 14 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 15 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 16 from 375 (out) to 397 (in) : 3683.74 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 68 (in) : 741.611 Transmembrane helix 4 from 72 (in) to 87 (out) : 66.86 Transmembrane helix 5 from 91 (out) to 106 (in) : 1323.86 Transmembrane helix 6 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 7 from 139 (out) to 154 (in) : -324.838 Transmembrane helix 8 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 9 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 10 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 11 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 12 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 13 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 14 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 15 from 375 (out) to 397 (in) : 3683.74 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 368.117 Transmembrane helix 2 from 34 (out) to 49 (in) : 457.255 Transmembrane helix 3 from 53 (in) to 68 (out) : 722.989 Transmembrane helix 4 from 72 (out) to 87 (in) : 72.14 Transmembrane helix 5 from 91 (in) to 106 (out) : 1353.94 Transmembrane helix 6 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 7 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 8 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 9 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 10 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 11 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 12 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 13 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 14 from 375 (out) to 397 (in) : 3683.74 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : 126.792 Transmembrane helix 2 from 31 (in) to 46 (out) : 667.972 Transmembrane helix 3 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 4 from 84 (in) to 104 (out) : 1615.73 Transmembrane helix 5 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 6 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 7 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 8 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 9 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 10 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 11 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 12 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 13 from 375 (out) to 397 (in) : 3683.74 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 20 (in) to 44 (out) : 1151.63 Transmembrane helix 2 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 3 from 84 (in) to 104 (out) : 1615.73 Transmembrane helix 4 from 111 (out) to 131 (in) : 1237.07 Transmembrane helix 5 from 158 (in) to 173 (out) : 821.119 Transmembrane helix 6 from 177 (out) to 194 (in) : 1849.58 Transmembrane helix 7 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 8 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 9 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 10 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 11 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 12 from 375 (out) to 397 (in) : 3683.74 Score = 22.220995 Processing 11 helices: Transmembrane helix 1 from 20 (out) to 45 (in) : 1104.74 Transmembrane helix 2 from 53 (in) to 74 (out) : 820.253 Transmembrane helix 3 from 84 (out) to 104 (in) : 1570.07 Transmembrane helix 4 from 111 (in) to 131 (out) : 1207.83 Transmembrane helix 5 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 6 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 7 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 8 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 9 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 10 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 11 from 375 (out) to 397 (in) : 3683.74 Score = 21.772268 Processing 10 helices: Transmembrane helix 1 from 20 (in) to 44 (out) : 1151.63 Transmembrane helix 2 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 3 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 4 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 5 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 6 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 7 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 8 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 9 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 10 from 375 (out) to 397 (in) : 3683.74 Score = 21.617998 Processing 9 helices: Transmembrane helix 1 from 20 (out) to 45 (in) : 1104.74 Transmembrane helix 2 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 3 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 4 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 5 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 6 from 289 (in) to 307 (out) : 2802.77 Transmembrane helix 7 from 311 (out) to 332 (in) : 2518.59 Transmembrane helix 8 from 349 (in) to 371 (out) : 940.377 Transmembrane helix 9 from 375 (out) to 397 (in) : 3683.74 Score = 20.576241 Processing 8 helices: Transmembrane helix 1 from 20 (in) to 44 (out) : 1151.63 Transmembrane helix 2 from 53 (out) to 75 (in) : 845.237 Transmembrane helix 3 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 4 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 5 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 6 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 7 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 8 from 366 (out) to 396 (in) : 4080.06 Score = 20.137119 Processing 7 helices: Transmembrane helix 1 from 20 (out) to 45 (in) : 1104.74 Transmembrane helix 2 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 3 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 4 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 5 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 6 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 7 from 366 (out) to 396 (in) : 4080.06 Score = 19.095362 Processing 6 helices: Transmembrane helix 1 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 2 from 163 (out) to 193 (in) : 2525.76 Transmembrane helix 3 from 226 (in) to 249 (out) : 2875.15 Transmembrane helix 4 from 259 (out) to 276 (in) : 1574.71 Transmembrane helix 5 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 6 from 366 (out) to 396 (in) : 4080.06 Score = 18.068890 Processing 5 helices: Transmembrane helix 1 from 91 (out) to 121 (in) : 2352.93 Transmembrane helix 2 from 163 (in) to 193 (out) : 2466.44 Transmembrane helix 3 from 226 (out) to 249 (in) : 2826.85 Transmembrane helix 4 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 5 from 366 (out) to 396 (in) : 4080.06 Score = 16.093214 Processing 4 helices: Transmembrane helix 1 from 89 (in) to 119 (out) : 2376.22 Transmembrane helix 2 from 226 (out) to 249 (in) : 2826.85 Transmembrane helix 3 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 4 from 366 (out) to 396 (in) : 4080.06 Score = 13.772587 Processing 3 helices: Transmembrane helix 1 from 226 (out) to 249 (in) : 2826.85 Transmembrane helix 2 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 3 from 366 (out) to 396 (in) : 4080.06 Score = 11.396364 Processing 2 helices: Transmembrane helix 1 from 292 (in) to 322 (out) : 4363.7 Transmembrane helix 2 from 366 (out) to 396 (in) : 4080.06 Score = 8.523789 Summary of topology analysis: 1 helix (+) : Score = 4.3637 1 helix (-) : Score = 4.30194 2 helices (+) : Score = 8.52379 2 helices (-) : Score = 8.24722 3 helices (+) : Score = 11.0747 3 helices (-) : Score = 11.3964 4 helices (+) : Score = 13.7726 4 helices (-) : Score = 13.6977 5 helices (+) : Score = 16.1033 5 helices (-) : Score = 16.0932 6 helices (+) : Score = 18.0689 6 helices (-) : Score = 17.2346 7 helices (+) : Score = 18.8218 7 helices (-) : Score = 19.0954 8 helices (+) : Score = 20.1371 8 helices (-) : Score = 19.8483 9 helices (+) : Score = 20.89 9 helices (-) : Score = 20.5762 10 helices (+) : Score = 21.618 10 helices (-) : Score = 21.0443 11 helices (+) : Score = 21.493 11 helices (-) : Score = 21.7723 12 helices (+) : Score = 22.221 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 1.935 Topology: 20-44,53-75,84-104,111-131,158-173,177-194,226-249,259-276,289-307,311-332,349-371,375-397 Re-entrant helices: Not detected. Helix count: 12 N-terminal: in Score: 22.221