BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (263 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P76612 Uncharacterized protein ypjB n=7 Tax=Enterobacte...   535   e-151
UniRef50_C3SYI8 Putative uncharacterized protein n=6 Tax=Escheri...   459   e-128

>UniRef50_P76612 Uncharacterized protein ypjB n=7 Tax=Enterobacteriaceae
           RepID=YPJB_ECOLI
          Length = 263

 Score =  535 bits (1377), Expect = e-151,   Method: Compositional matrix adjust.
 Identities = 263/263 (100%), Positives = 263/263 (100%)

Query: 1   MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC 60
           MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC
Sbjct: 1   MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC 60

Query: 61  YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK 120
           YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK
Sbjct: 61  YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK 120

Query: 121 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP 180
           NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP
Sbjct: 121 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP 180

Query: 181 CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE 240
           CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE
Sbjct: 181 CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE 240

Query: 241 DDPVENSPVVTSPVVSSSKSSFQ 263
           DDPVENSPVVTSPVVSSSKSSFQ
Sbjct: 241 DDPVENSPVVTSPVVSSSKSSFQ 263


>UniRef50_C3SYI8 Putative uncharacterized protein n=6 Tax=Escherichia coli
           RepID=C3SYI8_ECOLX
          Length = 449

 Score =  459 bits (1181), Expect = e-128,   Method: Compositional matrix adjust.
 Identities = 228/255 (89%), Positives = 241/255 (94%)

Query: 1   MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC 60
           ME RNSYENKIDE+SSLSES+EHPIDIQ+  DAFV EFKG+LFDKN RSSELLFNFYECC
Sbjct: 195 MEPRNSYENKIDEVSSLSESEEHPIDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECC 254

Query: 61  YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK 120
           Y FLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNN+GFDFKLFPEVKLSGEHLETVFKYK
Sbjct: 255 YTFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYK 314

Query: 121 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP 180
           NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWG SLFDVDTP
Sbjct: 315 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTP 374

Query: 181 CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE 240
           CIFN PA ++S EKSLKPVSENGL+GVL+DRN KIK+ITGVAPFDDILFMDDDFDD+S E
Sbjct: 375 CIFNTPANHESYEKSLKPVSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPE 434

Query: 241 DDPVENSPVVTSPVV 255
           D P+ENSPVV SP+V
Sbjct: 435 DAPIENSPVVNSPLV 449


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P76612 Uncharacterized protein ypjB n=7 Tax=Enterobacte...   494   e-138
UniRef50_C3SYI8 Putative uncharacterized protein n=6 Tax=Escheri...   492   e-138

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P76612 Uncharacterized protein ypjB n=7 Tax=Enterobacteriaceae
           RepID=YPJB_ECOLI
          Length = 263

 Score =  494 bits (1272), Expect = e-138,   Method: Composition-based stats.
 Identities = 263/263 (100%), Positives = 263/263 (100%)

Query: 1   MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC 60
           MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC
Sbjct: 1   MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC 60

Query: 61  YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK 120
           YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK
Sbjct: 61  YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK 120

Query: 121 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP 180
           NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP
Sbjct: 121 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP 180

Query: 181 CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE 240
           CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE
Sbjct: 181 CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE 240

Query: 241 DDPVENSPVVTSPVVSSSKSSFQ 263
           DDPVENSPVVTSPVVSSSKSSFQ
Sbjct: 241 DDPVENSPVVTSPVVSSSKSSFQ 263


>UniRef50_C3SYI8 Putative uncharacterized protein n=6 Tax=Escherichia coli
           RepID=C3SYI8_ECOLX
          Length = 449

 Score =  492 bits (1266), Expect = e-138,   Method: Composition-based stats.
 Identities = 228/255 (89%), Positives = 241/255 (94%)

Query: 1   MESRNSYENKIDEISSLSESKEHPIDIQEKKDAFVNEFKGVLFDKNTRSSELLFNFYECC 60
           ME RNSYENKIDE+SSLSES+EHPIDIQ+  DAFV EFKG+LFDKN RSSELLFNFYECC
Sbjct: 195 MEPRNSYENKIDEVSSLSESEEHPIDIQKITDAFVKEFKGILFDKNGRSSELLFNFYECC 254

Query: 61  YKFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNIGFDFKLFPEVKLSGEHLETVFKYK 120
           Y FLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNN+GFDFKLFPEVKLSGEHLETVFKYK
Sbjct: 255 YTFLPRAQPQDKIDSYNSALQAFSIFCSSTLTHNNVGFDFKLFPEVKLSGEHLETVFKYK 314

Query: 121 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGTSLFDVDTP 180
           NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWG SLFDVDTP
Sbjct: 315 NGDDVREIAKINITLQKEEGGLYNLRGLDFKGCFFSGQNFSNYDIQYVNWGMSLFDVDTP 374

Query: 181 CIFNAPAYNKSNEKSLKPVSENGLSGVLTDRNNKIKLITGVAPFDDILFMDDDFDDSSSE 240
           CIFN PA ++S EKSLKPVSENGL+GVL+DRN KIK+ITGVAPFDDILFMDDDFDD+S E
Sbjct: 375 CIFNTPANHESYEKSLKPVSENGLNGVLSDRNKKIKMITGVAPFDDILFMDDDFDDNSPE 434

Query: 241 DDPVENSPVVTSPVV 255
           D P+ENSPVV SP+V
Sbjct: 435 DAPIENSPVVNSPLV 449


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.312    0.140    0.426 

Lambda     K      H
   0.267   0.0437    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,147,008,288
Number of Sequences: 3077464
Number of extensions: 52415655
Number of successful extensions: 111173
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 111161
Number of HSP's gapped (non-prelim): 6
length of query: 263
length of database: 1,040,396,356
effective HSP length: 126
effective length of query: 137
effective length of database: 652,635,892
effective search space: 89411117204
effective search space used: 89411117204
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 92 (40.0 bits)