Processing 1 helix: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Score = 2.124184 Processing 1 helix: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Score = 2.139816 Processing 20 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -89.768 Transmembrane helix 2 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 3 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 4 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 5 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 6 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 7 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 8 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 9 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 10 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 11 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 12 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 13 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 14 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 15 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 16 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 17 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 18 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 19 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 20 from 1 (out) to 0 (in) : 3.779 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 20 (out) to 35 (in) : -262.603 Transmembrane helix 2 from 39 (in) to 54 (out) : 1467.02 Transmembrane helix 3 from 58 (out) to 73 (in) : -919.544 Transmembrane helix 4 from 77 (in) to 92 (out) : -299.569 Transmembrane helix 5 from 96 (out) to 111 (in) : 773.705 Transmembrane helix 6 from 115 (in) to 130 (out) : 580.383 Transmembrane helix 7 from 134 (out) to 149 (in) : 733.44 Transmembrane helix 8 from 153 (in) to 168 (out) : 590.692 Transmembrane helix 9 from 172 (out) to 187 (in) : 212.181 Transmembrane helix 10 from 191 (in) to 206 (out) : -334.882 Transmembrane helix 11 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 12 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 13 from 248 (out) to 263 (in) : -319.709 Transmembrane helix 14 from 267 (in) to 282 (out) : 1472.31 Transmembrane helix 15 from 286 (out) to 301 (in) : -351.976 Transmembrane helix 16 from 305 (in) to 320 (out) : 3.437 Transmembrane helix 17 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 18 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 19 from 362 (out) to 377 (in) : 0.185 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 32 (in) to 47 (out) : 1980.84 Transmembrane helix 2 from 51 (out) to 66 (in) : -709.754 Transmembrane helix 3 from 70 (in) to 85 (out) : -545.079 Transmembrane helix 4 from 89 (out) to 104 (in) : 246.23 Transmembrane helix 5 from 108 (in) to 123 (out) : 1138.21 Transmembrane helix 6 from 127 (out) to 142 (in) : 277.679 Transmembrane helix 7 from 146 (in) to 161 (out) : 569.963 Transmembrane helix 8 from 165 (out) to 180 (in) : 920.47 Transmembrane helix 9 from 191 (in) to 206 (out) : -334.882 Transmembrane helix 10 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 11 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 12 from 248 (out) to 263 (in) : -319.709 Transmembrane helix 13 from 267 (in) to 282 (out) : 1472.31 Transmembrane helix 14 from 286 (out) to 301 (in) : -351.976 Transmembrane helix 15 from 305 (in) to 320 (out) : 3.437 Transmembrane helix 16 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 17 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 18 from 362 (out) to 377 (in) : 0.185 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 69 (in) to 84 (out) : -581.951 Transmembrane helix 3 from 88 (out) to 103 (in) : 183.412 Transmembrane helix 4 from 107 (in) to 122 (out) : 1197.59 Transmembrane helix 5 from 126 (out) to 141 (in) : 234.175 Transmembrane helix 6 from 145 (in) to 160 (out) : 635.762 Transmembrane helix 7 from 164 (out) to 179 (in) : 957.29 Transmembrane helix 8 from 191 (in) to 206 (out) : -334.882 Transmembrane helix 9 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 10 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 11 from 248 (out) to 263 (in) : -319.709 Transmembrane helix 12 from 267 (in) to 282 (out) : 1472.31 Transmembrane helix 13 from 286 (out) to 301 (in) : -351.976 Transmembrane helix 14 from 305 (in) to 320 (out) : 3.437 Transmembrane helix 15 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 16 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 17 from 362 (out) to 377 (in) : 0.185 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 85 (out) to 100 (in) : 9.95799 Transmembrane helix 3 from 104 (in) to 119 (out) : 1221.65 Transmembrane helix 4 from 123 (out) to 138 (in) : 219.925 Transmembrane helix 5 from 142 (in) to 157 (out) : 843.664 Transmembrane helix 6 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 7 from 191 (in) to 206 (out) : -334.882 Transmembrane helix 8 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 9 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 10 from 248 (out) to 263 (in) : -319.709 Transmembrane helix 11 from 267 (in) to 282 (out) : 1472.31 Transmembrane helix 12 from 286 (out) to 301 (in) : -351.976 Transmembrane helix 13 from 305 (in) to 320 (out) : 3.437 Transmembrane helix 14 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 15 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 16 from 362 (out) to 377 (in) : 0.185 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 85 (in) to 100 (out) : 2.54199 Transmembrane helix 3 from 104 (out) to 119 (in) : 1218.15 Transmembrane helix 4 from 123 (in) to 138 (out) : 214.275 Transmembrane helix 5 from 142 (out) to 157 (in) : 838.036 Transmembrane helix 6 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 7 from 191 (out) to 206 (in) : -338.718 Transmembrane helix 8 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 9 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 10 from 248 (in) to 263 (out) : -308.391 Transmembrane helix 11 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 12 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 13 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 14 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 15 from 362 (out) to 377 (in) : 0.185 Re-entrant helix 1 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 85 (out) to 100 (in) : 9.95799 Transmembrane helix 3 from 104 (in) to 119 (out) : 1221.65 Transmembrane helix 4 from 123 (out) to 138 (in) : 219.925 Transmembrane helix 5 from 142 (in) to 157 (out) : 843.664 Transmembrane helix 6 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 7 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 8 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 9 from 248 (in) to 263 (out) : -308.391 Transmembrane helix 10 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 11 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 12 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 13 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 14 from 362 (out) to 377 (in) : 0.185 Re-entrant helix 1 from 183 (in) to 206 (in) : 1680 Re-entrant helix 2 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 85 (in) to 100 (out) : 2.54199 Transmembrane helix 3 from 104 (out) to 119 (in) : 1218.15 Transmembrane helix 4 from 123 (in) to 138 (out) : 214.275 Transmembrane helix 5 from 142 (out) to 157 (in) : 838.036 Transmembrane helix 6 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 7 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 8 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 9 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 10 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 11 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 12 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 13 from 362 (out) to 377 (in) : 0.185 Re-entrant helix 1 from 183 (out) to 206 (out) : 1680 Re-entrant helix 2 from 248 (out) to 263 (out) : 1120 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 88 (out) to 103 (in) : 183.412 Transmembrane helix 3 from 107 (in) to 124 (out) : 1232.01 Transmembrane helix 4 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 5 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 6 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 7 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 8 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 9 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 10 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 11 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 12 from 362 (out) to 377 (in) : 0.185 Re-entrant helix 1 from 183 (out) to 206 (out) : 1680 Re-entrant helix 2 from 248 (out) to 263 (out) : 1120 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 4 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 5 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 6 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 7 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 8 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 9 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 10 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 11 from 362 (out) to 377 (in) : 0.185 Re-entrant helix 1 from 183 (out) to 206 (out) : 1680 Re-entrant helix 2 from 248 (out) to 263 (out) : 1120 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 4 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 5 from 211 (in) to 238 (out) : 1013.06 Transmembrane helix 6 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 7 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 8 from 324 (out) to 339 (in) : -10.153 Transmembrane helix 9 from 343 (in) to 358 (out) : -2.892 Transmembrane helix 10 from 362 (out) to 377 (in) : 0.185 Re-entrant helix 1 from 183 (in) to 207 (in) : 1750 Re-entrant helix 2 from 242 (out) to 263 (out) : 1540 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 4 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 5 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 6 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 7 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 8 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 9 from 336 (out) to 351 (in) : 15.245 Re-entrant helix 1 from 183 (out) to 206 (out) : 1680 Re-entrant helix 2 from 248 (out) to 263 (out) : 1120 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Re-entrant helix 4 from 321 (out) to 332 (out) : 840 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 4 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 5 from 211 (in) to 238 (out) : 1013.06 Transmembrane helix 6 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 7 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 8 from 336 (out) to 351 (in) : 15.245 Re-entrant helix 1 from 183 (in) to 207 (in) : 1750 Re-entrant helix 2 from 242 (out) to 263 (out) : 1540 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Re-entrant helix 4 from 321 (out) to 332 (out) : 840 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 4 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 5 from 211 (out) to 237 (in) : 1011.55 Transmembrane helix 6 from 267 (in) to 286 (out) : 1631.73 Transmembrane helix 7 from 336 (out) to 351 (in) : 15.245 Re-entrant helix 1 from 183 (out) to 207 (out) : 1750 Re-entrant helix 2 from 241 (in) to 263 (in) : 1610 Re-entrant helix 3 from 290 (out) to 324 (out) : 2450 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 4 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 5 from 211 (in) to 238 (out) : 1013.06 Transmembrane helix 6 from 267 (out) to 286 (in) : 1647.47 Re-entrant helix 1 from 183 (in) to 207 (in) : 1750 Re-entrant helix 2 from 242 (out) to 263 (out) : 1540 Score = 8.450550 Processing 5 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 146 (out) to 176 (in) : 1457.03 Transmembrane helix 4 from 211 (in) to 238 (out) : 1013.06 Transmembrane helix 5 from 267 (out) to 286 (in) : 1647.47 Re-entrant helix 1 from 128 (out) to 142 (out) : 1050 Re-entrant helix 2 from 180 (in) to 207 (in) : 1960 Re-entrant helix 3 from 242 (out) to 263 (out) : 1540 Score = 7.814737 Processing 4 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 146 (in) to 176 (out) : 1448.87 Transmembrane helix 4 from 267 (out) to 286 (in) : 1647.47 Re-entrant helix 1 from 128 (in) to 142 (in) : 1050 Re-entrant helix 2 from 180 (out) to 214 (out) : 2450 Re-entrant helix 3 from 215 (out) to 249 (out) : 2450 Re-entrant helix 4 from 250 (out) to 263 (out) : 980 Score = 6.804632 Processing 3 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 267 (out) to 286 (in) : 1647.47 Re-entrant helix 1 from 128 (out) to 162 (out) : 2450 Re-entrant helix 2 from 163 (out) to 197 (out) : 2450 Re-entrant helix 3 from 198 (out) to 232 (out) : 2450 Re-entrant helix 4 from 233 (out) to 263 (out) : 2170 Score = 5.349584 Processing 2 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 267 (out) to 286 (in) : 1647.47 Re-entrant helix 1 from 112 (out) to 146 (out) : 2450 Re-entrant helix 2 from 147 (out) to 181 (out) : 2450 Re-entrant helix 3 from 182 (out) to 216 (out) : 2450 Re-entrant helix 4 from 217 (out) to 251 (out) : 2450 Re-entrant helix 5 from 252 (out) to 263 (out) : 840 Score = 3.771653 Processing 20 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -82.325 Transmembrane helix 2 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 3 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 4 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 5 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 6 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 7 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 8 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 9 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 10 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 11 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 12 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 13 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 14 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 15 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 16 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 17 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 18 from 1 (in) to 0 (out) : -4.46 Transmembrane helix 19 from 1 (out) to 0 (in) : 3.779 Transmembrane helix 20 from 1 (in) to 0 (out) : -4.46 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 20 (in) to 35 (out) : -274.097 Transmembrane helix 2 from 39 (out) to 54 (in) : 1445.48 Transmembrane helix 3 from 58 (in) to 73 (out) : -940.756 Transmembrane helix 4 from 77 (out) to 92 (in) : -305.631 Transmembrane helix 5 from 96 (in) to 111 (out) : 777.295 Transmembrane helix 6 from 115 (out) to 130 (in) : 590.417 Transmembrane helix 7 from 134 (in) to 149 (out) : 740.56 Transmembrane helix 8 from 153 (out) to 168 (in) : 592.008 Transmembrane helix 9 from 172 (in) to 187 (out) : 220.219 Transmembrane helix 10 from 191 (out) to 206 (in) : -338.718 Transmembrane helix 11 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 12 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 13 from 248 (in) to 263 (out) : -308.391 Transmembrane helix 14 from 267 (out) to 282 (in) : 1481.29 Transmembrane helix 15 from 286 (in) to 301 (out) : -344.224 Transmembrane helix 16 from 305 (out) to 320 (in) : -3.437 Transmembrane helix 17 from 324 (in) to 339 (out) : 10.153 Transmembrane helix 18 from 343 (out) to 358 (in) : 2.892 Transmembrane helix 19 from 362 (in) to 378 (out) : 0.344 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 32 (out) to 47 (in) : 1979.56 Transmembrane helix 2 from 51 (in) to 66 (out) : -719.446 Transmembrane helix 3 from 77 (out) to 92 (in) : -305.631 Transmembrane helix 4 from 96 (in) to 111 (out) : 777.295 Transmembrane helix 5 from 115 (out) to 130 (in) : 590.417 Transmembrane helix 6 from 134 (in) to 149 (out) : 740.56 Transmembrane helix 7 from 153 (out) to 168 (in) : 592.008 Transmembrane helix 8 from 172 (in) to 187 (out) : 220.219 Transmembrane helix 9 from 191 (out) to 206 (in) : -338.718 Transmembrane helix 10 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 11 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 12 from 248 (in) to 263 (out) : -308.391 Transmembrane helix 13 from 267 (out) to 282 (in) : 1481.29 Transmembrane helix 14 from 286 (in) to 301 (out) : -344.224 Transmembrane helix 15 from 305 (out) to 320 (in) : -3.437 Transmembrane helix 16 from 324 (in) to 339 (out) : 10.153 Transmembrane helix 17 from 343 (out) to 358 (in) : 2.892 Transmembrane helix 18 from 362 (in) to 378 (out) : 0.344 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 77 (out) to 92 (in) : -305.631 Transmembrane helix 3 from 96 (in) to 111 (out) : 777.295 Transmembrane helix 4 from 115 (out) to 130 (in) : 590.417 Transmembrane helix 5 from 134 (in) to 149 (out) : 740.56 Transmembrane helix 6 from 153 (out) to 168 (in) : 592.008 Transmembrane helix 7 from 172 (in) to 187 (out) : 220.219 Transmembrane helix 8 from 191 (out) to 206 (in) : -338.718 Transmembrane helix 9 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 10 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 11 from 248 (in) to 263 (out) : -308.391 Transmembrane helix 12 from 267 (out) to 282 (in) : 1481.29 Transmembrane helix 13 from 286 (in) to 301 (out) : -344.224 Transmembrane helix 14 from 305 (out) to 320 (in) : -3.437 Transmembrane helix 15 from 324 (in) to 339 (out) : 10.153 Transmembrane helix 16 from 343 (out) to 358 (in) : 2.892 Transmembrane helix 17 from 362 (in) to 378 (out) : 0.344 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 85 (in) to 100 (out) : 2.54199 Transmembrane helix 3 from 104 (out) to 119 (in) : 1218.15 Transmembrane helix 4 from 123 (in) to 138 (out) : 214.275 Transmembrane helix 5 from 142 (out) to 157 (in) : 838.036 Transmembrane helix 6 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 7 from 191 (out) to 206 (in) : -338.718 Transmembrane helix 8 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 9 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 10 from 248 (in) to 263 (out) : -308.391 Transmembrane helix 11 from 267 (out) to 282 (in) : 1481.29 Transmembrane helix 12 from 286 (in) to 301 (out) : -344.224 Transmembrane helix 13 from 305 (out) to 320 (in) : -3.437 Transmembrane helix 14 from 324 (in) to 339 (out) : 10.153 Transmembrane helix 15 from 343 (out) to 358 (in) : 2.892 Transmembrane helix 16 from 362 (in) to 378 (out) : 0.344 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 85 (out) to 100 (in) : 9.95799 Transmembrane helix 3 from 104 (in) to 119 (out) : 1221.65 Transmembrane helix 4 from 123 (out) to 138 (in) : 219.925 Transmembrane helix 5 from 142 (in) to 157 (out) : 843.664 Transmembrane helix 6 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 7 from 191 (in) to 206 (out) : -334.882 Transmembrane helix 8 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 9 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 10 from 249 (out) to 264 (in) : -308.652 Transmembrane helix 11 from 268 (in) to 286 (out) : 1622.02 Transmembrane helix 12 from 302 (out) to 317 (in) : -5.367 Transmembrane helix 13 from 321 (in) to 336 (out) : 8.528 Transmembrane helix 14 from 340 (out) to 355 (in) : 8.7 Transmembrane helix 15 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 290 (out) to 298 (out) : 630 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 85 (in) to 100 (out) : 2.54199 Transmembrane helix 3 from 104 (out) to 119 (in) : 1218.15 Transmembrane helix 4 from 123 (in) to 138 (out) : 214.275 Transmembrane helix 5 from 142 (out) to 157 (in) : 838.036 Transmembrane helix 6 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 7 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 8 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 9 from 249 (out) to 264 (in) : -308.652 Transmembrane helix 10 from 268 (in) to 286 (out) : 1622.02 Transmembrane helix 11 from 302 (out) to 317 (in) : -5.367 Transmembrane helix 12 from 321 (in) to 336 (out) : 8.528 Transmembrane helix 13 from 340 (out) to 355 (in) : 8.7 Transmembrane helix 14 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (out) to 206 (out) : 1680 Re-entrant helix 2 from 290 (out) to 298 (out) : 630 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 85 (out) to 100 (in) : 9.95799 Transmembrane helix 3 from 104 (in) to 119 (out) : 1221.65 Transmembrane helix 4 from 123 (out) to 138 (in) : 219.925 Transmembrane helix 5 from 142 (in) to 157 (out) : 843.664 Transmembrane helix 6 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 7 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 8 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 9 from 267 (in) to 286 (out) : 1631.73 Transmembrane helix 10 from 302 (out) to 317 (in) : -5.367 Transmembrane helix 11 from 321 (in) to 336 (out) : 8.528 Transmembrane helix 12 from 340 (out) to 355 (in) : 8.7 Transmembrane helix 13 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (in) to 206 (in) : 1680 Re-entrant helix 2 from 248 (in) to 263 (in) : 1120 Re-entrant helix 3 from 290 (out) to 298 (out) : 630 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 88 (in) to 103 (out) : 178.588 Transmembrane helix 3 from 107 (out) to 124 (in) : 1237.39 Transmembrane helix 4 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 5 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 6 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 7 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 8 from 267 (in) to 286 (out) : 1631.73 Transmembrane helix 9 from 302 (out) to 317 (in) : -5.367 Transmembrane helix 10 from 321 (in) to 336 (out) : 8.528 Transmembrane helix 11 from 340 (out) to 355 (in) : 8.7 Transmembrane helix 12 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (in) to 206 (in) : 1680 Re-entrant helix 2 from 248 (in) to 263 (in) : 1120 Re-entrant helix 3 from 290 (out) to 298 (out) : 630 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 4 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 5 from 210 (in) to 225 (out) : 784.577 Transmembrane helix 6 from 229 (out) to 244 (in) : 174.128 Transmembrane helix 7 from 267 (in) to 286 (out) : 1631.73 Transmembrane helix 8 from 302 (out) to 317 (in) : -5.367 Transmembrane helix 9 from 321 (in) to 336 (out) : 8.528 Transmembrane helix 10 from 340 (out) to 355 (in) : 8.7 Transmembrane helix 11 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (in) to 206 (in) : 1680 Re-entrant helix 2 from 248 (in) to 263 (in) : 1120 Re-entrant helix 3 from 290 (out) to 298 (out) : 630 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 4 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 5 from 210 (out) to 225 (in) : 792.623 Transmembrane helix 6 from 229 (in) to 244 (out) : 173.572 Transmembrane helix 7 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 8 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 9 from 336 (out) to 351 (in) : 15.245 Transmembrane helix 10 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (out) to 206 (out) : 1680 Re-entrant helix 2 from 248 (out) to 263 (out) : 1120 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Re-entrant helix 4 from 321 (out) to 332 (out) : 840 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 4 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 5 from 211 (in) to 238 (out) : 1013.06 Transmembrane helix 6 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 7 from 302 (in) to 317 (out) : 5.367 Transmembrane helix 8 from 336 (out) to 351 (in) : 15.245 Transmembrane helix 9 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (in) to 207 (in) : 1750 Re-entrant helix 2 from 242 (out) to 263 (out) : 1540 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Re-entrant helix 4 from 321 (out) to 332 (out) : 840 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 4 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 5 from 211 (out) to 237 (in) : 1011.55 Transmembrane helix 6 from 267 (in) to 286 (out) : 1631.73 Transmembrane helix 7 from 336 (out) to 351 (in) : 15.245 Transmembrane helix 8 from 362 (in) to 378 (out) : 0.344 Re-entrant helix 1 from 183 (out) to 207 (out) : 1750 Re-entrant helix 2 from 241 (in) to 263 (in) : 1610 Re-entrant helix 3 from 290 (out) to 324 (out) : 2450 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 134 (in) to 156 (out) : 1107.48 Transmembrane helix 4 from 163 (out) to 179 (in) : 976.346 Transmembrane helix 5 from 211 (in) to 238 (out) : 1013.06 Transmembrane helix 6 from 267 (out) to 286 (in) : 1647.47 Transmembrane helix 7 from 302 (in) to 318 (out) : 5.532 Re-entrant helix 1 from 183 (in) to 207 (in) : 1750 Re-entrant helix 2 from 242 (out) to 263 (out) : 1540 Re-entrant helix 3 from 290 (in) to 298 (in) : 630 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 134 (out) to 156 (in) : 1095.92 Transmembrane helix 4 from 163 (in) to 179 (out) : 964.854 Transmembrane helix 5 from 211 (out) to 237 (in) : 1011.55 Transmembrane helix 6 from 267 (in) to 286 (out) : 1631.73 Re-entrant helix 1 from 183 (out) to 207 (out) : 1750 Re-entrant helix 2 from 241 (in) to 263 (in) : 1610 Score = 8.393095 Processing 5 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 146 (in) to 176 (out) : 1448.87 Transmembrane helix 4 from 211 (out) to 237 (in) : 1011.55 Transmembrane helix 5 from 267 (in) to 286 (out) : 1631.73 Re-entrant helix 1 from 128 (in) to 142 (in) : 1050 Re-entrant helix 2 from 180 (out) to 207 (out) : 1960 Re-entrant helix 3 from 241 (in) to 263 (in) : 1610 Score = 7.790207 Processing 4 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 95 (in) to 124 (out) : 1564.89 Transmembrane helix 3 from 146 (out) to 176 (in) : 1457.03 Transmembrane helix 4 from 267 (in) to 286 (out) : 1631.73 Re-entrant helix 1 from 128 (out) to 142 (out) : 1050 Re-entrant helix 2 from 180 (in) to 214 (in) : 2450 Re-entrant helix 3 from 215 (in) to 249 (in) : 2450 Re-entrant helix 4 from 250 (in) to 263 (in) : 980 Score = 6.774468 Processing 3 helices: Transmembrane helix 1 from 32 (in) to 52 (out) : 2140 Transmembrane helix 2 from 95 (out) to 124 (in) : 1565.11 Transmembrane helix 3 from 267 (in) to 286 (out) : 1631.73 Re-entrant helix 1 from 128 (in) to 162 (in) : 2450 Re-entrant helix 2 from 163 (in) to 197 (in) : 2450 Re-entrant helix 3 from 198 (in) to 232 (in) : 2450 Re-entrant helix 4 from 233 (in) to 263 (in) : 2170 Score = 5.323617 Processing 2 helices: Transmembrane helix 1 from 32 (out) to 52 (in) : 2124 Transmembrane helix 2 from 267 (in) to 286 (out) : 1631.73 Re-entrant helix 1 from 112 (in) to 146 (in) : 2450 Re-entrant helix 2 from 147 (in) to 181 (in) : 2450 Re-entrant helix 3 from 182 (in) to 216 (in) : 2450 Re-entrant helix 4 from 217 (in) to 251 (in) : 2450 Re-entrant helix 5 from 252 (in) to 263 (in) : 840 Score = 3.771547 Summary of topology analysis: 1 helix (+) : Score = 2.12418 1 helix (-) : Score = 2.13982 2 helices (+) : Score = 3.77165 2 helices (-) : Score = 3.77155 3 helices (+) : Score = 5.32362 3 helices (-) : Score = 5.34958 4 helices (+) : Score = 6.80463 4 helices (-) : Score = 6.77447 5 helices (+) : Score = 7.79021 5 helices (-) : Score = 7.81474 6 helices (+) : Score = 8.45055 6 helices (-) : Score = 8.3931 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 32-52,95-124,134-156,163-179,211-238,267-286 Re-entrant helices: 183-207,242-263 Helix count: 6 N-terminal: in Score: 8.45055