BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (197 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P76547 Uncharacterized protein yffP n=3 Tax=Escherichia...   415   e-115
UniRef50_C4TIH9 Predicted protein n=1 Tax=Escherichia coli O157:...    97   4e-19

>UniRef50_P76547 Uncharacterized protein yffP n=3 Tax=Escherichia coli K-12
           RepID=YFFP_ECOLI
          Length = 197

 Score =  415 bits (1067), Expect = e-115,   Method: Compositional matrix adjust.
 Identities = 197/197 (100%), Positives = 197/197 (100%)

Query: 1   MSLIRTETRDTKRAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYTDTSSPFGIGMT 60
           MSLIRTETRDTKRAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYTDTSSPFGIGMT
Sbjct: 1   MSLIRTETRDTKRAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYTDTSSPFGIGMT 60

Query: 61  FDYTECWEIGVRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVS 120
           FDYTECWEIGVRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVS
Sbjct: 61  FDYTECWEIGVRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVS 120

Query: 121 LSHKDTFTGAKRFQYFQTMYDMERQINANLPVGGLPNVDTERTGKLFQRDDFSEDSHAND 180
           LSHKDTFTGAKRFQYFQTMYDMERQINANLPVGGLPNVDTERTGKLFQRDDFSEDSHAND
Sbjct: 121 LSHKDTFTGAKRFQYFQTMYDMERQINANLPVGGLPNVDTERTGKLFQRDDFSEDSHAND 180

Query: 181 PKLVGDDYVPQAPEQIN 197
           PKLVGDDYVPQAPEQIN
Sbjct: 181 PKLVGDDYVPQAPEQIN 197


>UniRef50_C4TIH9 Predicted protein n=1 Tax=Escherichia coli O157:H7
           RepID=C4TIH9_ECO57
          Length = 196

 Score = 96.7 bits (239), Expect = 4e-19,   Method: Compositional matrix adjust.
 Identities = 62/189 (32%), Positives = 98/189 (51%), Gaps = 10/189 (5%)

Query: 13  RAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYT--DTSSPFGIGMTFDYTECWEIG 70
           R  D    L +K FS++ ++++R+   ID  LE   T  +  S   +    D     E+ 
Sbjct: 13  RLEDGYSTLENKKFSDYTDEDLRKIGTIDVALELYSTNYEQGSQMALLEMADKINNHEVA 72

Query: 71  VRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVSLSHKDTFTGA 130
           + D+CL M R+ P  P    +   K V+ D     A  W G+ KV+A  SLS+KD+F   
Sbjct: 73  IIDNCLCMRRIAPPLPGMIHN---KAVLTDPA--FAYMWTGFVKVIAVHSLSYKDSFKAT 127

Query: 131 KRFQYFQTMYDMERQINANLPVGGLPNVDTE--RTGKLFQRDDFSEDSHAN-DPKLVGDD 187
            R +Y++ + D E+++N +LP+GG   VD +      L  R D+S++ H + +   V +D
Sbjct: 128 DRARYWRIVRDHEKRLNEDLPIGGYDAVDLQGIALANLRARPDYSDNPHKDCEGMFVPED 187

Query: 188 YVPQAPEQI 196
           YVP  PEQI
Sbjct: 188 YVPVPPEQI 196


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P76547 Uncharacterized protein yffP n=3 Tax=Escherichia...   348   8e-95
UniRef50_C4TIH9 Predicted protein n=1 Tax=Escherichia coli O157:...   283   2e-75

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P76547 Uncharacterized protein yffP n=3 Tax=Escherichia coli K-12
           RepID=YFFP_ECOLI
          Length = 197

 Score =  348 bits (892), Expect = 8e-95,   Method: Composition-based stats.
 Identities = 197/197 (100%), Positives = 197/197 (100%)

Query: 1   MSLIRTETRDTKRAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYTDTSSPFGIGMT 60
           MSLIRTETRDTKRAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYTDTSSPFGIGMT
Sbjct: 1   MSLIRTETRDTKRAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYTDTSSPFGIGMT 60

Query: 61  FDYTECWEIGVRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVS 120
           FDYTECWEIGVRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVS
Sbjct: 61  FDYTECWEIGVRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVS 120

Query: 121 LSHKDTFTGAKRFQYFQTMYDMERQINANLPVGGLPNVDTERTGKLFQRDDFSEDSHAND 180
           LSHKDTFTGAKRFQYFQTMYDMERQINANLPVGGLPNVDTERTGKLFQRDDFSEDSHAND
Sbjct: 121 LSHKDTFTGAKRFQYFQTMYDMERQINANLPVGGLPNVDTERTGKLFQRDDFSEDSHAND 180

Query: 181 PKLVGDDYVPQAPEQIN 197
           PKLVGDDYVPQAPEQIN
Sbjct: 181 PKLVGDDYVPQAPEQIN 197


>UniRef50_C4TIH9 Predicted protein n=1 Tax=Escherichia coli O157:H7
           RepID=C4TIH9_ECO57
          Length = 196

 Score =  283 bits (725), Expect = 2e-75,   Method: Composition-based stats.
 Identities = 62/189 (32%), Positives = 98/189 (51%), Gaps = 10/189 (5%)

Query: 13  RAADPLHDLRSKPFSEWGEDEIRRFNLIDALLEFVYT--DTSSPFGIGMTFDYTECWEIG 70
           R  D    L +K FS++ ++++R+   ID  LE   T  +  S   +    D     E+ 
Sbjct: 13  RLEDGYSTLENKKFSDYTDEDLRKIGTIDVALELYSTNYEQGSQMALLEMADKINNHEVA 72

Query: 71  VRDDCLVMTRVKPVHPEYAKHWNMKGVMNDKTRFHADKWVGYSKVLAWVSLSHKDTFTGA 130
           + D+CL M R+ P  P    +   K V+ D     A  W G+ KV+A  SLS+KD+F   
Sbjct: 73  IIDNCLCMRRIAPPLPGMIHN---KAVLTDPA--FAYMWTGFVKVIAVHSLSYKDSFKAT 127

Query: 131 KRFQYFQTMYDMERQINANLPVGGLPNVDTE--RTGKLFQRDDFSEDSHAN-DPKLVGDD 187
            R +Y++ + D E+++N +LP+GG   VD +      L  R D+S++ H + +   V +D
Sbjct: 128 DRARYWRIVRDHEKRLNEDLPIGGYDAVDLQGIALANLRARPDYSDNPHKDCEGMFVPED 187

Query: 188 YVPQAPEQI 196
           YVP  PEQI
Sbjct: 188 YVPVPPEQI 196


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.312    0.135    0.376 

Lambda     K      H
   0.267   0.0401    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 791,713,917
Number of Sequences: 3077464
Number of extensions: 30479608
Number of successful extensions: 61049
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 61040
Number of HSP's gapped (non-prelim): 4
length of query: 197
length of database: 1,040,396,356
effective HSP length: 122
effective length of query: 75
effective length of database: 664,945,748
effective search space: 49870931100
effective search space used: 49870931100
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 89 (38.9 bits)