BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (47 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_A4WBI4 Protein mgrB homolog n=89 Tax=Enterobacteriaceae...    82   7e-15
UniRef50_D2TM43 Putative exported protein n=1 Tax=Citrobacter ro...    80   2e-14

>UniRef50_A4WBI4 Protein mgrB homolog n=89 Tax=Enterobacteriaceae RepID=MGRB_ENT38
          Length = 47

 Score = 81.6 bits (200), Expect = 7e-15,   Method: Compositional matrix adjust.
 Identities = 35/47 (74%), Positives = 43/47 (91%)

Query: 1  MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQFIPW 47
          MKK RWV+L++V++AC++LW Q  N+MCDQDVQFFSGICAINQFIPW
Sbjct: 1  MKKIRWVILIIVLIACVILWTQTINVMCDQDVQFFSGICAINQFIPW 47


>UniRef50_D2TM43 Putative exported protein n=1 Tax=Citrobacter rodentium ICC168
          RepID=D2TM43_CITRO
          Length = 48

 Score = 80.5 bits (197), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 40/48 (83%), Positives = 44/48 (91%), Gaps = 1/48 (2%)

Query: 1  MKKFRWVVLV-VVVLACLLLWAQVFNMMCDQDVQFFSGICAINQFIPW 47
          MKKFRW++LV VVV  CLLLWAQV N+MCDQDVQFFSGICAIN+FIPW
Sbjct: 1  MKKFRWIILVMVVVPVCLLLWAQVVNLMCDQDVQFFSGICAINKFIPW 48


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_A4WBI4 Protein mgrB homolog n=89 Tax=Enterobacteriaceae...    56   3e-07
UniRef50_D2TM43 Putative exported protein n=1 Tax=Citrobacter ro...    54   2e-06

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_A4WBI4 Protein mgrB homolog n=89 Tax=Enterobacteriaceae RepID=MGRB_ENT38
          Length = 47

 Score = 56.0 bits (134), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 35/47 (74%), Positives = 43/47 (91%)

Query: 1  MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQFIPW 47
          MKK RWV+L++V++AC++LW Q  N+MCDQDVQFFSGICAINQFIPW
Sbjct: 1  MKKIRWVILIIVLIACVILWTQTINVMCDQDVQFFSGICAINQFIPW 47


>UniRef50_D2TM43 Putative exported protein n=1 Tax=Citrobacter rodentium ICC168
          RepID=D2TM43_CITRO
          Length = 48

 Score = 53.7 bits (128), Expect = 2e-06,   Method: Composition-based stats.
 Identities = 40/48 (83%), Positives = 44/48 (91%), Gaps = 1/48 (2%)

Query: 1  MKKFRWVVLV-VVVLACLLLWAQVFNMMCDQDVQFFSGICAINQFIPW 47
          MKKFRW++LV VVV  CLLLWAQV N+MCDQDVQFFSGICAIN+FIPW
Sbjct: 1  MKKFRWIILVMVVVPVCLLLWAQVVNLMCDQDVQFFSGICAINKFIPW 48


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.338    0.154    0.583 

Lambda     K      H
   0.267   0.0463    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 230,127,573
Number of Sequences: 3077464
Number of extensions: 6726967
Number of successful extensions: 36374
Number of sequences better than 1.0e-01: 6
Number of HSP's better than  0.1 without gapping: 9
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 36363
Number of HSP's gapped (non-prelim): 9
length of query: 47
length of database: 1,040,396,356
effective HSP length: 20
effective length of query: 27
effective length of database: 978,847,076
effective search space: 26428871052
effective search space used: 26428871052
T: 11
A: 40
X1: 16 ( 7.8 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.7 bits)
S2: 87 (37.9 bits)