Processing 1 helix: Transmembrane helix 1 from 141 (in) to 156 (out) : -100.984 Score = -0.096383 Processing 1 helix: Transmembrane helix 1 from 142 (out) to 157 (in) : -89.562 Score = -0.093199 Processing 8 helices: Transmembrane helix 1 from 12 (in) to 27 (out) : -903.693 Transmembrane helix 2 from 31 (out) to 46 (in) : -276.36 Transmembrane helix 3 from 50 (in) to 65 (out) : -501.745 Transmembrane helix 4 from 69 (out) to 84 (in) : -405.012 Transmembrane helix 5 from 88 (in) to 103 (out) : -726.862 Transmembrane helix 6 from 107 (out) to 122 (in) : -460.581 Transmembrane helix 7 from 126 (in) to 141 (out) : -342.643 Transmembrane helix 8 from 145 (out) to 160 (in) : -98.162 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -322.929 Transmembrane helix 2 from 46 (in) to 61 (out) : -426.92 Transmembrane helix 3 from 68 (out) to 83 (in) : -401.219 Transmembrane helix 4 from 88 (in) to 103 (out) : -726.862 Transmembrane helix 5 from 107 (out) to 122 (in) : -460.581 Transmembrane helix 6 from 126 (in) to 141 (out) : -342.643 Transmembrane helix 7 from 145 (out) to 160 (in) : -98.162 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -316.371 Transmembrane helix 2 from 46 (out) to 61 (in) : -436.88 Transmembrane helix 3 from 65 (in) to 80 (out) : -406.613 Transmembrane helix 4 from 107 (out) to 122 (in) : -460.581 Transmembrane helix 5 from 126 (in) to 141 (out) : -342.643 Transmembrane helix 6 from 145 (out) to 160 (in) : -98.162 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -272.23 Transmembrane helix 2 from 65 (in) to 80 (out) : -406.613 Transmembrane helix 3 from 107 (out) to 122 (in) : -460.581 Transmembrane helix 4 from 126 (in) to 141 (out) : -342.643 Transmembrane helix 5 from 145 (out) to 160 (in) : -98.162 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 32 (in) to 47 (out) : -267.569 Transmembrane helix 2 from 107 (out) to 122 (in) : -460.581 Transmembrane helix 3 from 126 (in) to 141 (out) : -342.643 Transmembrane helix 4 from 145 (out) to 160 (in) : -98.162 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -272.23 Transmembrane helix 2 from 67 (in) to 82 (out) : -401.12 Transmembrane helix 3 from 143 (out) to 158 (in) : -92.102 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -265.444 Transmembrane helix 2 from 142 (out) to 157 (in) : -89.562 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 12 (out) to 27 (in) : -915.407 Transmembrane helix 2 from 31 (in) to 46 (out) : -276.34 Transmembrane helix 3 from 50 (out) to 65 (in) : -498.855 Transmembrane helix 4 from 69 (in) to 84 (out) : -403.788 Transmembrane helix 5 from 88 (out) to 103 (in) : -733.738 Transmembrane helix 6 from 107 (in) to 122 (out) : -465.019 Transmembrane helix 7 from 126 (out) to 141 (in) : -335.657 Transmembrane helix 8 from 145 (in) to 160 (out) : -110.938 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -316.371 Transmembrane helix 2 from 46 (out) to 61 (in) : -436.88 Transmembrane helix 3 from 65 (in) to 80 (out) : -406.613 Transmembrane helix 4 from 88 (out) to 103 (in) : -733.738 Transmembrane helix 5 from 107 (in) to 122 (out) : -465.019 Transmembrane helix 6 from 126 (out) to 141 (in) : -335.657 Transmembrane helix 7 from 145 (in) to 160 (out) : -110.938 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 27 (out) to 42 (in) : -322.929 Transmembrane helix 2 from 46 (in) to 61 (out) : -426.92 Transmembrane helix 3 from 68 (out) to 83 (in) : -401.219 Transmembrane helix 4 from 107 (in) to 122 (out) : -465.019 Transmembrane helix 5 from 126 (out) to 141 (in) : -335.657 Transmembrane helix 6 from 145 (in) to 160 (out) : -110.938 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 27 (in) to 42 (out) : -316.371 Transmembrane helix 2 from 46 (out) to 61 (in) : -436.88 Transmembrane helix 3 from 65 (in) to 80 (out) : -406.613 Transmembrane helix 4 from 126 (out) to 141 (in) : -335.657 Transmembrane helix 5 from 145 (in) to 160 (out) : -110.938 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 35 (out) to 50 (in) : -272.23 Transmembrane helix 2 from 65 (in) to 80 (out) : -406.613 Transmembrane helix 3 from 126 (out) to 141 (in) : -335.657 Transmembrane helix 4 from 145 (in) to 160 (out) : -110.938 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 33 (in) to 48 (out) : -265.444 Transmembrane helix 2 from 126 (out) to 141 (in) : -335.657 Transmembrane helix 3 from 145 (in) to 160 (out) : -110.938 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 33 (out) to 48 (in) : -268.356 Transmembrane helix 2 from 141 (in) to 156 (out) : -100.984 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.096383 1 helix (-) : Score = -0.093199 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 142-157 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.093199