BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (86 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q79CP2 Putative uncharacterized protein ygiA n=18 Tax=E...   180   1e-44
UniRef50_UPI000190F518 hypothetical protein SentesTyph_05442 n=1...    56   4e-07

>UniRef50_Q79CP2 Putative uncharacterized protein ygiA n=18 Tax=Enterobacteriaceae
          RepID=YGIA_ECOLI
          Length = 86

 Score =  180 bits (457), Expect = 1e-44,   Method: Compositional matrix adjust.
 Identities = 86/86 (100%), Positives = 86/86 (100%)

Query: 1  MTTTGLRPRLNVRQRKDTGYLPHSSPFSLQFRPAILYSDGYLPLVPEDKNETDKIHTPRI 60
          MTTTGLRPRLNVRQRKDTGYLPHSSPFSLQFRPAILYSDGYLPLVPEDKNETDKIHTPRI
Sbjct: 1  MTTTGLRPRLNVRQRKDTGYLPHSSPFSLQFRPAILYSDGYLPLVPEDKNETDKIHTPRI 60

Query: 61 VPQKLERTPSDTSRSRGCHCFYAGWL 86
          VPQKLERTPSDTSRSRGCHCFYAGWL
Sbjct: 61 VPQKLERTPSDTSRSRGCHCFYAGWL 86


>UniRef50_UPI000190F518 hypothetical protein SentesTyph_05442 n=1 Tax=Salmonella enterica
          subsp. enterica serovar Typhi str. E98-0664
          RepID=UPI000190F518
          Length = 58

 Score = 55.8 bits (133), Expect = 4e-07,   Method: Compositional matrix adjust.
 Identities = 26/42 (61%), Positives = 28/42 (66%)

Query: 45 VPEDKNETDKIHTPRIVPQKLERTPSDTSRSRGCHCFYAGWL 86
          V EDK ETDKIH  RI+ QKLER   +  R  G  CFYAGWL
Sbjct: 17 VLEDKYETDKIHPSRIISQKLERAAFNAGRPGGYGCFYAGWL 58


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q79CP2 Putative uncharacterized protein ygiA n=18 Tax=E...   167   1e-40
UniRef50_UPI000190F518 hypothetical protein SentesTyph_05442 n=1...    88   8e-17

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_Q79CP2 Putative uncharacterized protein ygiA n=18 Tax=Enterobacteriaceae
          RepID=YGIA_ECOLI
          Length = 86

 Score =  167 bits (422), Expect = 1e-40,   Method: Composition-based stats.
 Identities = 86/86 (100%), Positives = 86/86 (100%)

Query: 1  MTTTGLRPRLNVRQRKDTGYLPHSSPFSLQFRPAILYSDGYLPLVPEDKNETDKIHTPRI 60
          MTTTGLRPRLNVRQRKDTGYLPHSSPFSLQFRPAILYSDGYLPLVPEDKNETDKIHTPRI
Sbjct: 1  MTTTGLRPRLNVRQRKDTGYLPHSSPFSLQFRPAILYSDGYLPLVPEDKNETDKIHTPRI 60

Query: 61 VPQKLERTPSDTSRSRGCHCFYAGWL 86
          VPQKLERTPSDTSRSRGCHCFYAGWL
Sbjct: 61 VPQKLERTPSDTSRSRGCHCFYAGWL 86


>UniRef50_UPI000190F518 hypothetical protein SentesTyph_05442 n=1 Tax=Salmonella enterica
          subsp. enterica serovar Typhi str. E98-0664
          RepID=UPI000190F518
          Length = 58

 Score = 88.1 bits (217), Expect = 8e-17,   Method: Composition-based stats.
 Identities = 26/42 (61%), Positives = 28/42 (66%)

Query: 45 VPEDKNETDKIHTPRIVPQKLERTPSDTSRSRGCHCFYAGWL 86
          V EDK ETDKIH  RI+ QKLER   +  R  G  CFYAGWL
Sbjct: 17 VLEDKYETDKIHPSRIISQKLERAAFNAGRPGGYGCFYAGWL 58


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.317    0.141    0.430 

Lambda     K      H
   0.267   0.0432    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 387,821,765
Number of Sequences: 3077464
Number of extensions: 15375837
Number of successful extensions: 29149
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 29145
Number of HSP's gapped (non-prelim): 4
length of query: 86
length of database: 1,040,396,356
effective HSP length: 56
effective length of query: 30
effective length of database: 868,058,372
effective search space: 26041751160
effective search space used: 26041751160
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 87 (38.0 bits)