BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (97 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q31US3 Uncharacterized protein yicS n=28 Tax=Enterobact...   204   8e-52
UniRef50_Q0TB41 Uncharacterized protein yicS n=62 Tax=Enterobact...   181   5e-45
UniRef50_A4W4V0 Putative uncharacterized protein n=2 Tax=Enterob...    81   9e-15
UniRef50_C4X3B7 Putative secreted protein n=4 Tax=Klebsiella Rep...    68   7e-11
UniRef50_C8QEH1 Putative uncharacterized protein n=1 Tax=Pantoea...    52   9e-06

>UniRef50_Q31US3 Uncharacterized protein yicS n=28 Tax=Enterobacteriaceae
          RepID=YICS_SHIBS
          Length = 97

 Score =  204 bits (518), Expect = 8e-52,   Method: Compositional matrix adjust.
 Identities = 97/97 (100%), Positives = 97/97 (100%)

Query: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
          MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM
Sbjct: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60

Query: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97
          LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM
Sbjct: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97


>UniRef50_Q0TB41 Uncharacterized protein yicS n=62 Tax=Enterobacteriaceae
          RepID=YICS_ECOL5
          Length = 97

 Score =  181 bits (460), Expect = 5e-45,   Method: Compositional matrix adjust.
 Identities = 87/97 (89%), Positives = 88/97 (90%)

Query: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
          MKPT LL+I  F   P I  AESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM
Sbjct: 1  MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60

Query: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97
          LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM
Sbjct: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97


>UniRef50_A4W4V0 Putative uncharacterized protein n=2 Tax=Enterobacter
          RepID=A4W4V0_ENT38
          Length = 98

 Score = 81.3 bits (199), Expect = 9e-15,   Method: Compositional matrix adjust.
 Identities = 35/94 (37%), Positives = 57/94 (60%)

Query: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
          MK   L  +          +A+      +  K K  V++D++K+C+PQ+  +D+ W+ ++
Sbjct: 1  MKVAHLFCLVVCLLFAAFAHAKETRDPAKDEKIKQVVMKDIKKVCSPQSKQTDKQWQTMI 60

Query: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVEC 94
          LS E NK  I+ A++AMER+N  NYWEA+GKV+C
Sbjct: 61 LSSEANKLLIKNAVLAMERDNLDNYWEAVGKVDC 94


>UniRef50_C4X3B7 Putative secreted protein n=4 Tax=Klebsiella RepID=C4X3B7_KLEPN
          Length = 116

 Score = 68.2 bits (165), Expect = 7e-11,   Method: Compositional matrix adjust.
 Identities = 35/95 (36%), Positives = 56/95 (58%), Gaps = 1/95 (1%)

Query: 1   MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
           MK   ++ + T  +     +AESP  SLQ  ++K  VL+ +++ C P + LSD  +   +
Sbjct: 21  MKACWMVCLVTALSATS-AWAESPLQSLQFEQQKQQVLKAVKEKCAPASHLSDNDFANKV 79

Query: 61  LSDENNKQHIREAIVAMERNNQSNYWEALGKVECP 95
           L+ + NK  +REA +A ERNNQ +Y  A+ K+ CP
Sbjct: 80  LATDGNKNAVREATLAKERNNQKSYQAAIDKIVCP 114


>UniRef50_C8QEH1 Putative uncharacterized protein n=1 Tax=Pantoea sp. At-9b
          RepID=C8QEH1_9ENTR
          Length = 102

 Score = 51.6 bits (122), Expect = 9e-06,   Method: Compositional matrix adjust.
 Identities = 25/93 (26%), Positives = 50/93 (53%), Gaps = 2/93 (2%)

Query: 4  TTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLMLSD 63
          T L+LI      PG+  A+S + +L  A  +  ++ DL+  C   A ++DE   ++ L+ 
Sbjct: 5  TLLVLIINLCPFPGM--AKSAYDNLAYALREQQIIGDLKLHCHIPAGVTDEHIRQVFLNS 62

Query: 64 ENNKQHIREAIVAMERNNQSNYWEALGKVECPD 96
          ++N   + +A  A++  +  +Y + + +V CPD
Sbjct: 63 KDNHDAVIDAASALKAQHHDSYQQQIARVRCPD 95


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q31US3 Uncharacterized protein yicS n=28 Tax=Enterobact...   150   1e-35
UniRef50_Q0TB41 Uncharacterized protein yicS n=62 Tax=Enterobact...   145   4e-34
UniRef50_A4W4V0 Putative uncharacterized protein n=2 Tax=Enterob...   127   1e-28
UniRef50_C8QEH1 Putative uncharacterized protein n=1 Tax=Pantoea...   126   2e-28
UniRef50_C4X3B7 Putative secreted protein n=4 Tax=Klebsiella Rep...   121   8e-27

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_Q31US3 Uncharacterized protein yicS n=28 Tax=Enterobacteriaceae
          RepID=YICS_SHIBS
          Length = 97

 Score =  150 bits (379), Expect = 1e-35,   Method: Composition-based stats.
 Identities = 97/97 (100%), Positives = 97/97 (100%)

Query: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
          MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM
Sbjct: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60

Query: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97
          LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM
Sbjct: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97


>UniRef50_Q0TB41 Uncharacterized protein yicS n=62 Tax=Enterobacteriaceae
          RepID=YICS_ECOL5
          Length = 97

 Score =  145 bits (365), Expect = 4e-34,   Method: Composition-based stats.
 Identities = 87/97 (89%), Positives = 88/97 (90%)

Query: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
          MKPT LL+I  F   P I  AESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM
Sbjct: 1  MKPTMLLMITVFLIFPAISQAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60

Query: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97
          LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM
Sbjct: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM 97


>UniRef50_A4W4V0 Putative uncharacterized protein n=2 Tax=Enterobacter
          RepID=A4W4V0_ENT38
          Length = 98

 Score =  127 bits (318), Expect = 1e-28,   Method: Composition-based stats.
 Identities = 35/94 (37%), Positives = 57/94 (60%)

Query: 1  MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
          MK   L  +          +A+      +  K K  V++D++K+C+PQ+  +D+ W+ ++
Sbjct: 1  MKVAHLFCLVVCLLFAAFAHAKETRDPAKDEKIKQVVMKDIKKVCSPQSKQTDKQWQTMI 60

Query: 61 LSDENNKQHIREAIVAMERNNQSNYWEALGKVEC 94
          LS E NK  I+ A++AMER+N  NYWEA+GKV+C
Sbjct: 61 LSSEANKLLIKNAVLAMERDNLDNYWEAVGKVDC 94


>UniRef50_C8QEH1 Putative uncharacterized protein n=1 Tax=Pantoea sp. At-9b
          RepID=C8QEH1_9ENTR
          Length = 102

 Score =  126 bits (316), Expect = 2e-28,   Method: Composition-based stats.
 Identities = 25/93 (26%), Positives = 50/93 (53%), Gaps = 2/93 (2%)

Query: 4  TTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLMLSD 63
          T L+LI      PG+  A+S + +L  A  +  ++ DL+  C   A ++DE   ++ L+ 
Sbjct: 5  TLLVLIINLCPFPGM--AKSAYDNLAYALREQQIIGDLKLHCHIPAGVTDEHIRQVFLNS 62

Query: 64 ENNKQHIREAIVAMERNNQSNYWEALGKVECPD 96
          ++N   + +A  A++  +  +Y + + +V CPD
Sbjct: 63 KDNHDAVIDAASALKAQHHDSYQQQIARVRCPD 95


>UniRef50_C4X3B7 Putative secreted protein n=4 Tax=Klebsiella RepID=C4X3B7_KLEPN
          Length = 116

 Score =  121 bits (303), Expect = 8e-27,   Method: Composition-based stats.
 Identities = 35/95 (36%), Positives = 56/95 (58%), Gaps = 1/95 (1%)

Query: 1   MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKICTPQASLSDEAWEKLM 60
           MK   ++ + T  +     +AESP  SLQ  ++K  VL+ +++ C P + LSD  +   +
Sbjct: 21  MKACWMVCLVTALSATS-AWAESPLQSLQFEQQKQQVLKAVKEKCAPASHLSDNDFANKV 79

Query: 61  LSDENNKQHIREAIVAMERNNQSNYWEALGKVECP 95
           L+ + NK  +REA +A ERNNQ +Y  A+ K+ CP
Sbjct: 80  LATDGNKNAVREATLAKERNNQKSYQAAIDKIVCP 114


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.305    0.123    0.314 

Lambda     K      H
   0.267   0.0389    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 304,010,630
Number of Sequences: 3077464
Number of extensions: 8393372
Number of successful extensions: 27584
Number of sequences better than 1.0e-01: 5
Number of HSP's better than  0.1 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 27571
Number of HSP's gapped (non-prelim): 10
length of query: 97
length of database: 1,040,396,356
effective HSP length: 66
effective length of query: 31
effective length of database: 837,283,732
effective search space: 25955795692
effective search space used: 25955795692
T: 11
A: 40
X1: 16 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.0 bits)
S2: 87 (38.2 bits)
