BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (70 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AAQ1 Uncharacterized protein yaiZ n=70 Tax=Enterobact...   143   2e-33
UniRef50_A7MLV4 Putative uncharacterized protein n=32 Tax=Entero...   117   2e-25

>UniRef50_P0AAQ1 Uncharacterized protein yaiZ n=70 Tax=Enterobacteriaceae
          RepID=YAIZ_ECOL6
          Length = 70

 Score =  143 bits (360), Expect = 2e-33,   Method: Compositional matrix adjust.
 Identities = 70/70 (100%), Positives = 70/70 (100%)

Query: 1  MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR 60
          MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR
Sbjct: 1  MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR 60

Query: 61 RRDEETENAQ 70
          RRDEETENAQ
Sbjct: 61 RRDEETENAQ 70


>UniRef50_A7MLV4 Putative uncharacterized protein n=32 Tax=Enterobacteriaceae
          RepID=A7MLV4_ENTS8
          Length = 84

 Score =  117 bits (292), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 53/70 (75%), Positives = 61/70 (87%)

Query: 1  MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR 60
          M L  KIRRDWH+YA A+GLIFI+NGV+GLLGFEAKGWQTYAVGLVTW+I FW+AG IIR
Sbjct: 14 MQLSAKIRRDWHFYAVALGLIFIMNGVIGLLGFEAKGWQTYAVGLVTWIICFWIAGFIIR 73

Query: 61 RRDEETENAQ 70
          RR EE+  A+
Sbjct: 74 RRPEESNAAE 83


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_A7MLV4 Putative uncharacterized protein n=32 Tax=Entero...   117   1e-25
UniRef50_P0AAQ1 Uncharacterized protein yaiZ n=70 Tax=Enterobact...   113   1e-24

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_A7MLV4 Putative uncharacterized protein n=32 Tax=Enterobacteriaceae
          RepID=A7MLV4_ENTS8
          Length = 84

 Score =  117 bits (293), Expect = 1e-25,   Method: Composition-based stats.
 Identities = 53/70 (75%), Positives = 61/70 (87%)

Query: 1  MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR 60
          M L  KIRRDWH+YA A+GLIFI+NGV+GLLGFEAKGWQTYAVGLVTW+I FW+AG IIR
Sbjct: 14 MQLSAKIRRDWHFYAVALGLIFIMNGVIGLLGFEAKGWQTYAVGLVTWIICFWIAGFIIR 73

Query: 61 RRDEETENAQ 70
          RR EE+  A+
Sbjct: 74 RRPEESNAAE 83


>UniRef50_P0AAQ1 Uncharacterized protein yaiZ n=70 Tax=Enterobacteriaceae
          RepID=YAIZ_ECOL6
          Length = 70

 Score =  113 bits (284), Expect = 1e-24,   Method: Composition-based stats.
 Identities = 70/70 (100%), Positives = 70/70 (100%)

Query: 1  MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR 60
          MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR
Sbjct: 1  MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVGLVTWVISFWLAGLIIR 60

Query: 61 RRDEETENAQ 70
          RRDEETENAQ
Sbjct: 61 RRDEETENAQ 70


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.328    0.154    0.514 

Lambda     K      H
   0.267   0.0460    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 351,563,126
Number of Sequences: 3077464
Number of extensions: 13192834
Number of successful extensions: 42560
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 42556
Number of HSP's gapped (non-prelim): 4
length of query: 70
length of database: 1,040,396,356
effective HSP length: 42
effective length of query: 28
effective length of database: 911,142,868
effective search space: 25512000304
effective search space used: 25512000304
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 87 (38.0 bits)