Processing 1 helix: Transmembrane helix 1 from 106 (in) to 133 (out) : 3910.94 Score = 3.910940 Processing 1 helix: Transmembrane helix 1 from 106 (out) to 136 (in) : 3907.62 Score = 3.907622 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 1272.46 Transmembrane helix 2 from 23 (out) to 38 (in) : -361.143 Transmembrane helix 3 from 44 (in) to 59 (out) : 729.762 Transmembrane helix 4 from 63 (out) to 78 (in) : 1476.53 Transmembrane helix 5 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 6 from 103 (out) to 118 (in) : 1322.85 Transmembrane helix 7 from 122 (in) to 146 (out) : 2337.41 Transmembrane helix 8 from 150 (out) to 165 (in) : -7.585 Transmembrane helix 9 from 169 (in) to 184 (out) : -11.464 Transmembrane helix 10 from 188 (out) to 203 (in) : -1.767 Transmembrane helix 11 from 207 (in) to 222 (out) : 1.106 Transmembrane helix 12 from 226 (out) to 241 (in) : -1.155 Transmembrane helix 13 from 245 (in) to 260 (out) : 8.602 Transmembrane helix 14 from 264 (out) to 279 (in) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 44 (in) to 59 (out) : 729.762 Transmembrane helix 3 from 63 (out) to 78 (in) : 1476.53 Transmembrane helix 4 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 5 from 103 (out) to 118 (in) : 1322.85 Transmembrane helix 6 from 122 (in) to 146 (out) : 2337.41 Transmembrane helix 7 from 150 (out) to 165 (in) : -7.585 Transmembrane helix 8 from 169 (in) to 184 (out) : -11.464 Transmembrane helix 9 from 188 (out) to 203 (in) : -1.767 Transmembrane helix 10 from 207 (in) to 222 (out) : 1.106 Transmembrane helix 11 from 226 (out) to 241 (in) : -1.155 Transmembrane helix 12 from 245 (in) to 260 (out) : 8.602 Transmembrane helix 13 from 264 (out) to 279 (in) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 103 (out) to 118 (in) : 1322.85 Transmembrane helix 5 from 122 (in) to 146 (out) : 2337.41 Transmembrane helix 6 from 150 (out) to 165 (in) : -7.585 Transmembrane helix 7 from 169 (in) to 184 (out) : -11.464 Transmembrane helix 8 from 188 (out) to 203 (in) : -1.767 Transmembrane helix 9 from 207 (in) to 222 (out) : 1.106 Transmembrane helix 10 from 226 (out) to 241 (in) : -1.155 Transmembrane helix 11 from 245 (in) to 260 (out) : 8.602 Transmembrane helix 12 from 264 (out) to 279 (in) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 137 (out) to 152 (in) : -15.142 Transmembrane helix 6 from 156 (in) to 171 (out) : -11.233 Transmembrane helix 7 from 175 (out) to 190 (in) : 0.766 Transmembrane helix 8 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 9 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 10 from 245 (in) to 260 (out) : 8.602 Transmembrane helix 11 from 264 (out) to 279 (in) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 7 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 8 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 9 from 245 (in) to 260 (out) : 8.602 Transmembrane helix 10 from 264 (out) to 279 (in) : 0 Re-entrant helix 1 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 6 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 7 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 8 from 245 (in) to 260 (out) : 8.602 Transmembrane helix 9 from 264 (out) to 279 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 183 (in) : 24.313 Transmembrane helix 7 from 244 (in) to 260 (out) : 8.934 Transmembrane helix 8 from 264 (out) to 279 (in) : 0 Re-entrant helix 1 from 187 (in) to 221 (in) : 2450 Re-entrant helix 2 from 222 (in) to 240 (in) : 1330 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 183 (in) : 24.313 Transmembrane helix 6 from 244 (in) to 260 (out) : 8.934 Transmembrane helix 7 from 264 (out) to 279 (in) : 0 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 187 (in) to 221 (in) : 2450 Re-entrant helix 3 from 222 (in) to 240 (in) : 1330 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 187 (in) : 26.723 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 187 (in) : 26.723 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Score = 9.896234 Processing 3 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 61 (in) to 91 (out) : 3132.32 Transmembrane helix 3 from 106 (out) to 136 (in) : 3907.62 Score = 8.652244 Processing 2 helices: Transmembrane helix 1 from 61 (in) to 91 (out) : 3132.32 Transmembrane helix 2 from 106 (out) to 136 (in) : 3907.62 Score = 7.040433 Processing 14 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 1290.34 Transmembrane helix 2 from 23 (in) to 38 (out) : -354.257 Transmembrane helix 3 from 42 (out) to 57 (in) : 406.578 Transmembrane helix 4 from 61 (in) to 76 (out) : 1811.76 Transmembrane helix 5 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 6 from 103 (in) to 118 (out) : 1341.35 Transmembrane helix 7 from 122 (out) to 137 (in) : 2371.05 Transmembrane helix 8 from 141 (in) to 156 (out) : 14.721 Transmembrane helix 9 from 163 (out) to 178 (in) : 19.064 Transmembrane helix 10 from 182 (in) to 197 (out) : 7.482 Transmembrane helix 11 from 201 (out) to 216 (in) : -2.422 Transmembrane helix 12 from 220 (in) to 235 (out) : 1.655 Transmembrane helix 13 from 239 (out) to 254 (in) : 2.718 Transmembrane helix 14 from 258 (in) to 273 (out) : -0.596 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 42 (out) to 57 (in) : 406.578 Transmembrane helix 3 from 61 (in) to 76 (out) : 1811.76 Transmembrane helix 4 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 5 from 103 (in) to 118 (out) : 1341.35 Transmembrane helix 6 from 122 (out) to 137 (in) : 2371.05 Transmembrane helix 7 from 141 (in) to 156 (out) : 14.721 Transmembrane helix 8 from 163 (out) to 178 (in) : 19.064 Transmembrane helix 9 from 182 (in) to 197 (out) : 7.482 Transmembrane helix 10 from 201 (out) to 216 (in) : -2.422 Transmembrane helix 11 from 220 (in) to 235 (out) : 1.655 Transmembrane helix 12 from 239 (out) to 254 (in) : 2.718 Transmembrane helix 13 from 258 (in) to 273 (out) : -0.596 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 103 (in) to 118 (out) : 1341.35 Transmembrane helix 5 from 122 (out) to 137 (in) : 2371.05 Transmembrane helix 6 from 141 (in) to 156 (out) : 14.721 Transmembrane helix 7 from 163 (out) to 178 (in) : 19.064 Transmembrane helix 8 from 182 (in) to 197 (out) : 7.482 Transmembrane helix 9 from 201 (out) to 216 (in) : -2.422 Transmembrane helix 10 from 220 (in) to 235 (out) : 1.655 Transmembrane helix 11 from 239 (out) to 254 (in) : 2.718 Transmembrane helix 12 from 258 (in) to 273 (out) : -0.596 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 163 (out) to 178 (in) : 19.064 Transmembrane helix 7 from 182 (in) to 197 (out) : 7.482 Transmembrane helix 8 from 201 (out) to 216 (in) : -2.422 Transmembrane helix 9 from 220 (in) to 235 (out) : 1.655 Transmembrane helix 10 from 239 (out) to 254 (in) : 2.718 Transmembrane helix 11 from 258 (in) to 273 (out) : -0.596 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 163 (out) to 178 (in) : 19.064 Transmembrane helix 6 from 182 (in) to 197 (out) : 7.482 Transmembrane helix 7 from 201 (out) to 216 (in) : -2.422 Transmembrane helix 8 from 220 (in) to 235 (out) : 1.655 Transmembrane helix 9 from 239 (out) to 254 (in) : 2.718 Transmembrane helix 10 from 258 (in) to 273 (out) : -0.596 Re-entrant helix 1 from 137 (out) to 159 (out) : 1610 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 7 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 8 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 9 from 248 (in) to 263 (out) : 8.572 Re-entrant helix 1 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 179 (in) : 18.514 Transmembrane helix 6 from 203 (in) to 218 (out) : 3.203 Transmembrane helix 7 from 222 (out) to 237 (in) : -1.584 Transmembrane helix 8 from 248 (in) to 263 (out) : 8.572 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 183 (in) to 199 (in) : 1190 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 155 (out) : 15.47 Transmembrane helix 6 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 7 from 246 (in) to 263 (out) : 10.385 Re-entrant helix 1 from 189 (in) to 223 (in) : 2450 Re-entrant helix 2 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Transmembrane helix 5 from 164 (out) to 185 (in) : 26.161 Transmembrane helix 6 from 246 (in) to 263 (out) : 10.385 Re-entrant helix 1 from 137 (out) to 160 (out) : 1680 Re-entrant helix 2 from 189 (in) to 223 (in) : 2450 Re-entrant helix 3 from 224 (in) to 242 (in) : 1330 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 50 (out) to 76 (in) : 2594.29 Transmembrane helix 3 from 82 (in) to 99 (out) : 1869.84 Transmembrane helix 4 from 106 (out) to 136 (in) : 3907.62 Transmembrane helix 5 from 140 (in) to 163 (out) : 27.097 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 4 (out) to 25 (in) : 1589.87 Transmembrane helix 2 from 50 (in) to 75 (out) : 2618.48 Transmembrane helix 3 from 82 (out) to 99 (in) : 1892.36 Transmembrane helix 4 from 106 (in) to 133 (out) : 3910.94 Score = 10.045128 Processing 3 helices: Transmembrane helix 1 from 4 (in) to 25 (out) : 1556.13 Transmembrane helix 2 from 61 (out) to 91 (in) : 3110.58 Transmembrane helix 3 from 106 (in) to 133 (out) : 3910.94 Score = 8.555220 Processing 2 helices: Transmembrane helix 1 from 61 (out) to 91 (in) : 3110.58 Transmembrane helix 2 from 106 (in) to 133 (out) : 3910.94 Score = 7.021029 Summary of topology analysis: 1 helix (+) : Score = 3.91094 1 helix (-) : Score = 3.90762 2 helices (+) : Score = 7.04043 2 helices (-) : Score = 7.02103 3 helices (+) : Score = 8.55522 3 helices (-) : Score = 8.65224 4 helices (+) : Score = 9.89623 4 helices (-) : Score = 10.0451 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0.249 Topology: 4-25,50-75,82-99,106-133 Re-entrant helices: Not detected. Helix count: 4 N-terminal: out Score: 10.0451