BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (63 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P0AAN7 Uncharacterized protein yaiA n=103 Tax=Enterobac...   129   2e-29
UniRef50_C9XX52 Uncharacterized protein yaiA n=2 Tax=Cronobacter...   107   1e-22
UniRef50_A8GAI5 Putative uncharacterized protein n=2 Tax=Serrati...    48   9e-05

>UniRef50_P0AAN7 Uncharacterized protein yaiA n=103 Tax=Enterobacteriaceae
          RepID=YAIA_ECO57
          Length = 63

 Score =  129 bits (325), Expect = 2e-29,   Method: Compositional matrix adjust.
 Identities = 63/63 (100%), Positives = 63/63 (100%)

Query: 1  MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60
          MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP
Sbjct: 1  MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60

Query: 61 DKE 63
          DKE
Sbjct: 61 DKE 63


>UniRef50_C9XX52 Uncharacterized protein yaiA n=2 Tax=Cronobacter
          RepID=C9XX52_CROTZ
          Length = 63

 Score =  107 bits (267), Expect = 1e-22,   Method: Compositional matrix adjust.
 Identities = 50/62 (80%), Positives = 57/62 (91%)

Query: 1  MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60
          MPT+PPYPREA +V +EKG PG+TVT YQLRADHP+PDSLISEHPT QEA+DAK+RYEDP
Sbjct: 1  MPTRPPYPREARVVPVEKGVPGKTVTSYQLRADHPEPDSLISEHPTEQEALDAKRRYEDP 60

Query: 61 DK 62
          DK
Sbjct: 61 DK 62


>UniRef50_A8GAI5 Putative uncharacterized protein n=2 Tax=Serratia
          RepID=A8GAI5_SERP5
          Length = 42

 Score = 48.1 bits (113), Expect = 9e-05,   Method: Compositional matrix adjust.
 Identities = 20/33 (60%), Positives = 28/33 (84%)

Query: 28 YQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60
          +++R D  +P++L+SEHPT QEA+DAK RYEDP
Sbjct: 6  FEVRTDDIEPNTLLSEHPTEQEALDAKHRYEDP 38


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P0AAN7 Uncharacterized protein yaiA n=103 Tax=Enterobac...    96   4e-19
UniRef50_C9XX52 Uncharacterized protein yaiA n=2 Tax=Cronobacter...    90   3e-17
UniRef50_A8GAI5 Putative uncharacterized protein n=2 Tax=Serrati...    56   3e-07

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P0AAN7 Uncharacterized protein yaiA n=103 Tax=Enterobacteriaceae
          RepID=YAIA_ECO57
          Length = 63

 Score = 95.6 bits (236), Expect = 4e-19,   Method: Composition-based stats.
 Identities = 63/63 (100%), Positives = 63/63 (100%)

Query: 1  MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60
          MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP
Sbjct: 1  MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60

Query: 61 DKE 63
          DKE
Sbjct: 61 DKE 63


>UniRef50_C9XX52 Uncharacterized protein yaiA n=2 Tax=Cronobacter
          RepID=C9XX52_CROTZ
          Length = 63

 Score = 89.8 bits (221), Expect = 3e-17,   Method: Composition-based stats.
 Identities = 50/62 (80%), Positives = 57/62 (91%)

Query: 1  MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEHPTAQEAMDAKKRYEDP 60
          MPT+PPYPREA +V +EKG PG+TVT YQLRADHP+PDSLISEHPT QEA+DAK+RYEDP
Sbjct: 1  MPTRPPYPREARVVPVEKGVPGKTVTSYQLRADHPEPDSLISEHPTEQEALDAKRRYEDP 60

Query: 61 DK 62
          DK
Sbjct: 61 DK 62


>UniRef50_A8GAI5 Putative uncharacterized protein n=2 Tax=Serratia
          RepID=A8GAI5_SERP5
          Length = 42

 Score = 56.3 bits (134), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 21/36 (58%), Positives = 29/36 (80%)

Query: 28 YQLRADHPKPDSLISEHPTAQEAMDAKKRYEDPDKE 63
          +++R D  +P++L+SEHPT QEA+DAK RYEDP  E
Sbjct: 6  FEVRTDDIEPNTLLSEHPTEQEALDAKHRYEDPALE 41


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.293    0.129    0.385 

Lambda     K      H
   0.267   0.0400    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 289,356,034
Number of Sequences: 3077464
Number of extensions: 9164706
Number of successful extensions: 15770
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 15764
Number of HSP's gapped (non-prelim): 6
length of query: 63
length of database: 1,040,396,356
effective HSP length: 35
effective length of query: 28
effective length of database: 932,685,116
effective search space: 26115183248
effective search space used: 26115183248
T: 11
A: 40
X1: 16 ( 6.8 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (20.7 bits)
S2: 87 (38.2 bits)