Processing 1 helix: Transmembrane helix 1 from 85 (in) to 103 (out) : 464.991 Score = 0.464991 Processing 1 helix: Transmembrane helix 1 from 85 (out) to 103 (in) : 477.209 Score = 0.477209 Processing 21 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 61 (out) to 76 (in) : -1255.04 Transmembrane helix 5 from 80 (in) to 95 (out) : 247.978 Transmembrane helix 6 from 99 (out) to 114 (in) : 69.501 Transmembrane helix 7 from 118 (in) to 133 (out) : -300.273 Transmembrane helix 8 from 137 (out) to 152 (in) : -529.277 Transmembrane helix 9 from 156 (in) to 171 (out) : -876.963 Transmembrane helix 10 from 175 (out) to 190 (in) : -738.854 Transmembrane helix 11 from 194 (in) to 209 (out) : -808.088 Transmembrane helix 12 from 213 (out) to 228 (in) : -98.152 Transmembrane helix 13 from 232 (in) to 247 (out) : -788.882 Transmembrane helix 14 from 251 (out) to 266 (in) : -777.014 Transmembrane helix 15 from 270 (in) to 285 (out) : -203.192 Transmembrane helix 16 from 292 (out) to 307 (in) : -808.191 Transmembrane helix 17 from 311 (in) to 326 (out) : -490.866 Transmembrane helix 18 from 330 (out) to 345 (in) : -615.11 Transmembrane helix 19 from 349 (in) to 364 (out) : -604.013 Transmembrane helix 20 from 368 (out) to 383 (in) : -1101.66 Transmembrane helix 21 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 74 (in) to 89 (out) : -121.134 Transmembrane helix 5 from 93 (out) to 108 (in) : 193.047 Transmembrane helix 6 from 112 (in) to 127 (out) : -71.331 Transmembrane helix 7 from 131 (out) to 146 (in) : -544.58 Transmembrane helix 8 from 150 (in) to 165 (out) : -793.412 Transmembrane helix 9 from 169 (out) to 184 (in) : -727.82 Transmembrane helix 10 from 188 (in) to 203 (out) : -764.429 Transmembrane helix 11 from 207 (out) to 222 (in) : -339.374 Transmembrane helix 12 from 226 (in) to 241 (out) : -467.193 Transmembrane helix 13 from 245 (out) to 260 (in) : -932.748 Transmembrane helix 14 from 264 (in) to 279 (out) : -201.858 Transmembrane helix 15 from 283 (out) to 298 (in) : -860.907 Transmembrane helix 16 from 302 (in) to 317 (out) : -654.548 Transmembrane helix 17 from 321 (out) to 336 (in) : -476.859 Transmembrane helix 18 from 340 (in) to 355 (out) : -568.992 Transmembrane helix 19 from 359 (out) to 374 (in) : -866.092 Transmembrane helix 20 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 75 (out) to 90 (in) : -25.164 Transmembrane helix 5 from 94 (in) to 109 (out) : 186.874 Transmembrane helix 6 from 113 (out) to 128 (in) : -120.297 Transmembrane helix 7 from 132 (in) to 147 (out) : -549.902 Transmembrane helix 8 from 151 (out) to 166 (in) : -805.92 Transmembrane helix 9 from 171 (in) to 186 (out) : -708.279 Transmembrane helix 10 from 191 (out) to 206 (in) : -747.671 Transmembrane helix 11 from 210 (in) to 225 (out) : -186.23 Transmembrane helix 12 from 229 (out) to 244 (in) : -641.544 Transmembrane helix 13 from 250 (in) to 265 (out) : -816.343 Transmembrane helix 14 from 269 (out) to 284 (in) : -163.7 Transmembrane helix 15 from 292 (in) to 307 (out) : -808.509 Transmembrane helix 16 from 311 (out) to 326 (in) : -487.734 Transmembrane helix 17 from 330 (in) to 345 (out) : -613.09 Transmembrane helix 18 from 349 (out) to 364 (in) : -605.387 Transmembrane helix 19 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 5 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 6 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 7 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 8 from 167 (in) to 182 (out) : -765.485 Transmembrane helix 9 from 186 (out) to 201 (in) : -728.213 Transmembrane helix 10 from 206 (in) to 221 (out) : -377.131 Transmembrane helix 11 from 225 (out) to 240 (in) : -411.702 Transmembrane helix 12 from 250 (in) to 265 (out) : -816.343 Transmembrane helix 13 from 269 (out) to 284 (in) : -163.7 Transmembrane helix 14 from 292 (in) to 307 (out) : -808.509 Transmembrane helix 15 from 311 (out) to 326 (in) : -487.734 Transmembrane helix 16 from 330 (in) to 345 (out) : -613.09 Transmembrane helix 17 from 349 (out) to 364 (in) : -605.387 Transmembrane helix 18 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 5 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 6 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 7 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 8 from 167 (out) to 182 (in) : -754.815 Transmembrane helix 9 from 186 (in) to 201 (out) : -717.087 Transmembrane helix 10 from 206 (out) to 221 (in) : -387.669 Transmembrane helix 11 from 225 (in) to 240 (out) : -406.698 Transmembrane helix 12 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 13 from 292 (in) to 307 (out) : -808.509 Transmembrane helix 14 from 311 (out) to 326 (in) : -487.734 Transmembrane helix 15 from 330 (in) to 345 (out) : -613.09 Transmembrane helix 16 from 349 (out) to 364 (in) : -605.387 Transmembrane helix 17 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 5 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 6 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 7 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 8 from 181 (in) to 196 (out) : -657.719 Transmembrane helix 9 from 206 (out) to 221 (in) : -387.669 Transmembrane helix 10 from 225 (in) to 240 (out) : -406.698 Transmembrane helix 11 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 12 from 292 (in) to 307 (out) : -808.509 Transmembrane helix 13 from 311 (out) to 326 (in) : -487.734 Transmembrane helix 14 from 330 (in) to 345 (out) : -613.09 Transmembrane helix 15 from 349 (out) to 364 (in) : -605.387 Transmembrane helix 16 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 5 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 6 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 7 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 8 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 9 from 206 (in) to 221 (out) : -377.131 Transmembrane helix 10 from 225 (out) to 240 (in) : -411.702 Transmembrane helix 11 from 267 (in) to 282 (out) : -144.791 Transmembrane helix 12 from 301 (out) to 316 (in) : -657.833 Transmembrane helix 13 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 14 from 341 (out) to 356 (in) : -569.155 Transmembrane helix 15 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 7 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 8 from 206 (in) to 221 (out) : -377.131 Transmembrane helix 9 from 225 (out) to 240 (in) : -411.702 Transmembrane helix 10 from 267 (in) to 282 (out) : -144.791 Transmembrane helix 11 from 301 (out) to 316 (in) : -657.833 Transmembrane helix 12 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 13 from 341 (out) to 356 (in) : -569.155 Transmembrane helix 14 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 5 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 6 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 7 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 8 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 9 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 10 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 11 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 12 from 341 (out) to 356 (in) : -569.155 Transmembrane helix 13 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 7 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 8 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 9 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 10 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 11 from 341 (out) to 356 (in) : -569.155 Transmembrane helix 12 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 29 (out) to 44 (in) : -430.646 Transmembrane helix 3 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 4 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 5 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 6 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 7 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 8 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 9 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 10 from 341 (out) to 356 (in) : -569.155 Transmembrane helix 11 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 7 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 8 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 9 from 341 (out) to 356 (in) : -569.155 Transmembrane helix 10 from 387 (in) to 402 (out) : -591.826 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 29 (out) to 44 (in) : -430.646 Transmembrane helix 3 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 4 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 5 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 6 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 7 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 8 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 9 from 321 (in) to 336 (out) : -466.941 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 7 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 8 from 321 (in) to 336 (out) : -466.941 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 3 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 4 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 5 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 6 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 7 from 321 (in) to 336 (out) : -466.941 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 85 (out) to 103 (in) : 477.209 Transmembrane helix 4 from 108 (in) to 123 (out) : 39.93 Transmembrane helix 5 from 216 (out) to 231 (in) : -62.455 Transmembrane helix 6 from 267 (in) to 282 (out) : -144.791 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 85 (out) to 103 (in) : 477.209 Transmembrane helix 3 from 108 (in) to 123 (out) : 39.93 Transmembrane helix 4 from 216 (out) to 231 (in) : -62.455 Transmembrane helix 5 from 267 (in) to 282 (out) : -144.791 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : 232.489 Transmembrane helix 2 from 85 (in) to 103 (out) : 464.991 Transmembrane helix 3 from 108 (out) to 123 (in) : 27.87 Transmembrane helix 4 from 216 (in) to 231 (out) : -53.845 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 85 (out) to 103 (in) : 477.209 Transmembrane helix 3 from 108 (in) to 123 (out) : 39.93 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : 232.489 Transmembrane helix 2 from 85 (in) to 103 (out) : 464.991 Score = 0.699202 Processing 21 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 61 (in) to 76 (out) : -1246.76 Transmembrane helix 5 from 80 (out) to 95 (in) : 246.022 Transmembrane helix 6 from 99 (in) to 114 (out) : 73.899 Transmembrane helix 7 from 118 (out) to 133 (in) : -301.427 Transmembrane helix 8 from 137 (in) to 152 (out) : -527.123 Transmembrane helix 9 from 156 (out) to 171 (in) : -875.437 Transmembrane helix 10 from 175 (in) to 190 (out) : -739.346 Transmembrane helix 11 from 194 (out) to 209 (in) : -787.512 Transmembrane helix 12 from 213 (in) to 228 (out) : -88.448 Transmembrane helix 13 from 232 (out) to 247 (in) : -782.318 Transmembrane helix 14 from 251 (in) to 266 (out) : -777.286 Transmembrane helix 15 from 270 (out) to 285 (in) : -202.908 Transmembrane helix 16 from 292 (in) to 307 (out) : -808.509 Transmembrane helix 17 from 311 (out) to 326 (in) : -487.734 Transmembrane helix 18 from 330 (in) to 345 (out) : -613.09 Transmembrane helix 19 from 349 (out) to 364 (in) : -605.387 Transmembrane helix 20 from 368 (in) to 383 (out) : -1106.94 Transmembrane helix 21 from 387 (out) to 402 (in) : -594.674 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 74 (out) to 89 (in) : -101.366 Transmembrane helix 5 from 93 (in) to 108 (out) : 187.053 Transmembrane helix 6 from 112 (out) to 127 (in) : -79.369 Transmembrane helix 7 from 131 (in) to 146 (out) : -542.42 Transmembrane helix 8 from 150 (out) to 165 (in) : -788.788 Transmembrane helix 9 from 169 (in) to 184 (out) : -735.48 Transmembrane helix 10 from 188 (out) to 203 (in) : -769.871 Transmembrane helix 11 from 207 (in) to 222 (out) : -327.026 Transmembrane helix 12 from 226 (out) to 241 (in) : -471.207 Transmembrane helix 13 from 245 (in) to 260 (out) : -919.552 Transmembrane helix 14 from 264 (out) to 279 (in) : -194.542 Transmembrane helix 15 from 283 (in) to 298 (out) : -864.293 Transmembrane helix 16 from 302 (out) to 317 (in) : -657.052 Transmembrane helix 17 from 321 (in) to 336 (out) : -466.941 Transmembrane helix 18 from 340 (out) to 355 (in) : -572.708 Transmembrane helix 19 from 359 (in) to 374 (out) : -871.808 Transmembrane helix 20 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 75 (in) to 90 (out) : -41.736 Transmembrane helix 5 from 94 (out) to 109 (in) : 189.626 Transmembrane helix 6 from 113 (in) to 128 (out) : -114.303 Transmembrane helix 7 from 132 (out) to 147 (in) : -550.398 Transmembrane helix 8 from 151 (in) to 166 (out) : -811.38 Transmembrane helix 9 from 171 (out) to 186 (in) : -704.121 Transmembrane helix 10 from 190 (in) to 205 (out) : -751.738 Transmembrane helix 11 from 209 (out) to 224 (in) : -243.698 Transmembrane helix 12 from 228 (in) to 243 (out) : -592.58 Transmembrane helix 13 from 251 (out) to 266 (in) : -777.014 Transmembrane helix 14 from 270 (in) to 285 (out) : -203.192 Transmembrane helix 15 from 292 (out) to 307 (in) : -808.191 Transmembrane helix 16 from 311 (in) to 326 (out) : -490.866 Transmembrane helix 17 from 330 (out) to 345 (in) : -615.11 Transmembrane helix 18 from 349 (in) to 364 (out) : -604.013 Transmembrane helix 19 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 5 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 6 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 7 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 8 from 167 (out) to 182 (in) : -754.815 Transmembrane helix 9 from 186 (in) to 201 (out) : -717.087 Transmembrane helix 10 from 206 (out) to 221 (in) : -387.669 Transmembrane helix 11 from 225 (in) to 240 (out) : -406.698 Transmembrane helix 12 from 251 (out) to 266 (in) : -777.014 Transmembrane helix 13 from 270 (in) to 285 (out) : -203.192 Transmembrane helix 14 from 292 (out) to 307 (in) : -808.191 Transmembrane helix 15 from 311 (in) to 326 (out) : -490.866 Transmembrane helix 16 from 330 (out) to 345 (in) : -615.11 Transmembrane helix 17 from 349 (in) to 364 (out) : -604.013 Transmembrane helix 18 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 5 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 6 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 7 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 8 from 167 (in) to 182 (out) : -765.485 Transmembrane helix 9 from 186 (out) to 201 (in) : -728.213 Transmembrane helix 10 from 206 (in) to 221 (out) : -377.131 Transmembrane helix 11 from 225 (out) to 240 (in) : -411.702 Transmembrane helix 12 from 250 (in) to 265 (out) : -816.343 Transmembrane helix 13 from 269 (out) to 284 (in) : -163.7 Transmembrane helix 14 from 309 (in) to 324 (out) : -534.503 Transmembrane helix 15 from 328 (out) to 343 (in) : -587.034 Transmembrane helix 16 from 347 (in) to 362 (out) : -569.383 Transmembrane helix 17 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 5 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 6 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 7 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 8 from 167 (out) to 182 (in) : -754.815 Transmembrane helix 9 from 186 (in) to 201 (out) : -717.087 Transmembrane helix 10 from 206 (out) to 221 (in) : -387.669 Transmembrane helix 11 from 225 (in) to 240 (out) : -406.698 Transmembrane helix 12 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 13 from 309 (in) to 324 (out) : -534.503 Transmembrane helix 14 from 328 (out) to 343 (in) : -587.034 Transmembrane helix 15 from 347 (in) to 362 (out) : -569.383 Transmembrane helix 16 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : 94.518 Transmembrane helix 2 from 23 (in) to 38 (out) : -328.255 Transmembrane helix 3 from 42 (out) to 57 (in) : -678.665 Transmembrane helix 4 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 5 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 6 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 7 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 8 from 181 (in) to 196 (out) : -657.719 Transmembrane helix 9 from 206 (out) to 221 (in) : -387.669 Transmembrane helix 10 from 225 (in) to 240 (out) : -406.698 Transmembrane helix 11 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 12 from 309 (in) to 324 (out) : -534.503 Transmembrane helix 13 from 328 (out) to 343 (in) : -587.034 Transmembrane helix 14 from 347 (in) to 362 (out) : -569.383 Transmembrane helix 15 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : 86.682 Transmembrane helix 2 from 23 (out) to 38 (in) : -326.645 Transmembrane helix 3 from 42 (in) to 57 (out) : -665.535 Transmembrane helix 4 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 5 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 6 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 7 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 8 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 9 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 10 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 11 from 309 (in) to 324 (out) : -534.503 Transmembrane helix 12 from 328 (out) to 343 (in) : -587.034 Transmembrane helix 13 from 347 (in) to 362 (out) : -569.383 Transmembrane helix 14 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 7 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 8 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 9 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 10 from 309 (in) to 324 (out) : -534.503 Transmembrane helix 11 from 328 (out) to 343 (in) : -587.034 Transmembrane helix 12 from 347 (in) to 362 (out) : -569.383 Transmembrane helix 13 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 29 (out) to 44 (in) : -430.646 Transmembrane helix 3 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 4 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 5 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 6 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 7 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 8 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 9 from 309 (in) to 324 (out) : -534.503 Transmembrane helix 10 from 328 (out) to 343 (in) : -587.034 Transmembrane helix 11 from 347 (in) to 362 (out) : -569.383 Transmembrane helix 12 from 386 (out) to 401 (in) : -596.43 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 138 (in) to 153 (out) : -525.601 Transmembrane helix 7 from 181 (out) to 196 (in) : -660.881 Transmembrane helix 8 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 9 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 10 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 11 from 341 (out) to 356 (in) : -569.155 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 29 (out) to 44 (in) : -430.646 Transmembrane helix 3 from 77 (in) to 92 (out) : 62.311 Transmembrane helix 4 from 96 (out) to 111 (in) : 171.666 Transmembrane helix 5 from 115 (in) to 130 (out) : -184.177 Transmembrane helix 6 from 138 (out) to 153 (in) : -527.099 Transmembrane helix 7 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 8 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 9 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 10 from 341 (out) to 356 (in) : -569.155 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 7 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 8 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 9 from 341 (out) to 356 (in) : -569.155 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 3 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 4 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 5 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 6 from 267 (out) to 282 (in) : -141.809 Transmembrane helix 7 from 322 (in) to 337 (out) : -467.942 Transmembrane helix 8 from 341 (out) to 356 (in) : -569.155 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 9 (out) to 24 (in) : 239.167 Transmembrane helix 2 from 32 (in) to 47 (out) : -420.8 Transmembrane helix 3 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 4 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 5 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 6 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 7 from 267 (out) to 282 (in) : -141.809 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 77 (out) to 92 (in) : 70.589 Transmembrane helix 3 from 96 (in) to 111 (out) : 172.034 Transmembrane helix 4 from 115 (out) to 130 (in) : -188.623 Transmembrane helix 5 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 6 from 267 (out) to 282 (in) : -141.809 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : 232.489 Transmembrane helix 2 from 85 (in) to 103 (out) : 464.991 Transmembrane helix 3 from 108 (out) to 123 (in) : 27.87 Transmembrane helix 4 from 216 (in) to 231 (out) : -53.845 Transmembrane helix 5 from 267 (out) to 282 (in) : -141.809 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 85 (out) to 103 (in) : 477.209 Transmembrane helix 3 from 108 (in) to 123 (out) : 39.93 Transmembrane helix 4 from 216 (out) to 231 (in) : -62.455 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 8 (out) to 23 (in) : 232.489 Transmembrane helix 2 from 85 (in) to 103 (out) : 464.991 Transmembrane helix 3 from 108 (out) to 123 (in) : 27.87 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 9 (in) to 24 (out) : 233.733 Transmembrane helix 2 from 85 (out) to 103 (in) : 477.209 Score = 0.718457 Summary of topology analysis: 1 helix (+) : Score = 0.464991 1 helix (-) : Score = 0.477209 2 helices (+) : Score = 0.718457 2 helices (-) : Score = 0.699202 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 9-24,85-103 Re-entrant helices: Not detected. Helix count: 2 N-terminal: in Score: 0.718457