BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (99 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P64491 Uncharacterized protein yoaC n=86 Tax=Enterobact...   204   9e-52
UniRef50_A4WEQ0 Putative uncharacterized protein n=2 Tax=Enterob...   157   1e-37

>UniRef50_P64491 Uncharacterized protein yoaC n=86 Tax=Enterobacteriaceae
          RepID=YOAC_ECO57
          Length = 99

 Score =  204 bits (518), Expect = 9e-52,   Method: Compositional matrix adjust.
 Identities = 99/99 (100%), Positives = 99/99 (100%)

Query: 1  MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP 60
          MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP
Sbjct: 1  MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP 60

Query: 61 GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV 99
          GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV
Sbjct: 61 GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV 99


>UniRef50_A4WEQ0 Putative uncharacterized protein n=2 Tax=Enterobacteriaceae
          RepID=A4WEQ0_ENT38
          Length = 99

 Score =  157 bits (397), Expect = 1e-37,   Method: Compositional matrix adjust.
 Identities = 72/99 (72%), Positives = 85/99 (85%)

Query: 1  MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP 60
          MPAVIDKALDFIG MD SA  P SM+ESTAKG+FKYLKE+GVPASA D+TAR  QEGW+ 
Sbjct: 1  MPAVIDKALDFIGGMDTSASAPQSMDESTAKGMFKYLKEIGVPASADDVTARGTQEGWDA 60

Query: 61 GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV 99
          GFT+K+ GWA+K+E+G   VIKNPEYFS YM+E+L+ALV
Sbjct: 61 GFTQKVAGWAEKIESGSHIVIKNPEYFSAYMREQLRALV 99


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_A4WEQ0 Putative uncharacterized protein n=2 Tax=Enterob...   181   7e-45
UniRef50_P64491 Uncharacterized protein yoaC n=86 Tax=Enterobact...   180   2e-44

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_A4WEQ0 Putative uncharacterized protein n=2 Tax=Enterobacteriaceae
          RepID=A4WEQ0_ENT38
          Length = 99

 Score =  181 bits (459), Expect = 7e-45,   Method: Composition-based stats.
 Identities = 72/99 (72%), Positives = 85/99 (85%)

Query: 1  MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP 60
          MPAVIDKALDFIG MD SA  P SM+ESTAKG+FKYLKE+GVPASA D+TAR  QEGW+ 
Sbjct: 1  MPAVIDKALDFIGGMDTSASAPQSMDESTAKGMFKYLKEIGVPASADDVTARGTQEGWDA 60

Query: 61 GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV 99
          GFT+K+ GWA+K+E+G   VIKNPEYFS YM+E+L+ALV
Sbjct: 61 GFTQKVAGWAEKIESGSHIVIKNPEYFSAYMREQLRALV 99


>UniRef50_P64491 Uncharacterized protein yoaC n=86 Tax=Enterobacteriaceae
          RepID=YOAC_ECO57
          Length = 99

 Score =  180 bits (456), Expect = 2e-44,   Method: Composition-based stats.
 Identities = 99/99 (100%), Positives = 99/99 (100%)

Query: 1  MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP 60
          MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP
Sbjct: 1  MPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKYLKELGVPASAADITARADQEGWNP 60

Query: 61 GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV 99
          GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV
Sbjct: 61 GFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEELKALV 99


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.309    0.135    0.388 

Lambda     K      H
   0.267   0.0394    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 359,908,418
Number of Sequences: 3077464
Number of extensions: 12129748
Number of successful extensions: 26036
Number of sequences better than 1.0e-01: 2
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 26032
Number of HSP's gapped (non-prelim): 4
length of query: 99
length of database: 1,040,396,356
effective HSP length: 68
effective length of query: 31
effective length of database: 831,128,804
effective search space: 25764992924
effective search space used: 25764992924
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.6 bits)
S2: 87 (38.2 bits)