BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (231 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_B8E544 7-cyano-7-deazaguanine synthase n=198 Tax=Bacter...   332   8e-90
UniRef50_Q1LTK2 7-cyano-7-deazaguanine synthase n=234 Tax=Bacter...   328   6e-89
UniRef50_Q8K986 7-cyano-7-deazaguanine synthase n=1 Tax=Buchnera...   244   2e-63
UniRef50_A6LIS9 7-cyano-7-deazaguanine synthase n=88 Tax=Bacteri...   214   1e-54
UniRef50_A6VQW6 7-cyano-7-deazaguanine synthase n=22 Tax=Pasteur...   212   9e-54
UniRef50_B4E7W5 7-cyano-7-deazaguanine synthase n=73 Tax=Bacteri...   200   4e-50
UniRef50_C1D6L0 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria...   183   5e-45
UniRef50_A5A3S4 BcepGomrgp35 n=1 Tax=Burkholderia phage BcepGomr...   182   6e-45
UniRef50_Q7M8H4 7-cyano-7-deazaguanine synthase n=2 Tax=Campylob...   182   7e-45
UniRef50_Q0BQ87 7-cyano-7-deazaguanine synthase n=76 Tax=Bacteri...   179   8e-44
UniRef50_C6XGK1 ExsB protein n=9 Tax=Bacteria RepID=C6XGK1_LIBAP      177   3e-43
UniRef50_Q7NCE2 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria...   172   1e-41
UniRef50_Q1CTN3 7-cyano-7-deazaguanine synthase n=12 Tax=Helicob...   168   1e-40
UniRef50_D0MDN1 ExsB protein n=1 Tax=Rhodothermus marinus DSM 42...   162   6e-39
UniRef50_B2KDX5 ExsB protein n=1 Tax=Elusimicrobium minutum Pei1...   148   2e-34
UniRef50_Q1QCP3 7-cyano-7-deazaguanine synthase n=122 Tax=Bacter...   142   8e-33
UniRef50_A7HHA2 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria...   141   2e-32
UniRef50_A0LJR5 7-cyano-7-deazaguanine synthase n=4 Tax=Bacteria...   135   1e-30
UniRef50_B2I4Y4 7-cyano-7-deazaguanine synthase n=16 Tax=Bacteri...   133   5e-30
UniRef50_UPI0001C171C7 ExsB n=2 Tax=Nostocaceae RepID=UPI0001C171C7   132   7e-30
UniRef50_A8GPK3 7-cyano-7-deazaguanine synthase n=18 Tax=Proteob...   132   8e-30
UniRef50_D1R584 Putative uncharacterized protein n=1 Tax=Parachl...   132   1e-29
UniRef50_UPI00016988F1 exsB protein n=1 Tax=Endoriftia persephon...   130   4e-29
UniRef50_A4XFR8 7-cyano-7-deazaguanine synthase n=2 Tax=cellular...   127   2e-28
UniRef50_B0TFM0 Exsb protein, putative n=1 Tax=Heliobacterium mo...   127   3e-28
UniRef50_Q1QVW9 7-cyano-7-deazaguanine synthase n=23 Tax=Proteob...   126   8e-28
UniRef50_Q2N612 7-cyano-7-deazaguanine synthase n=56 Tax=Bacteri...   124   3e-27
UniRef50_B0S9S9 7-cyano-7-deazaguanine synthase n=13 Tax=Bacteri...   123   4e-27
UniRef50_C8R2H0 ExsB protein n=1 Tax=Desulfurivibrio alkaliphilu...   123   6e-27
UniRef50_A8EWF5 7-cyano-7-deazaguanine synthase n=27 Tax=Epsilon...   122   1e-26
UniRef50_C0QST3 7-cyano-7-deazaguanine synthase n=7 Tax=Aquifica...   119   6e-26
UniRef50_Q8YM45 7-cyano-7-deazaguanine synthase n=14 Tax=cellula...   117   2e-25
UniRef50_Q6ARX9 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria...   116   7e-25
UniRef50_B9MMB0 7-cyano-7-deazaguanine synthase n=8 Tax=Bacteria...   116   7e-25
UniRef50_B9Z9V1 ExsB protein n=2 Tax=Halobacteriaceae RepID=B9Z9...   116   7e-25
UniRef50_Q55468 7-cyano-7-deazaguanine synthase n=43 Tax=Bacteri...   114   4e-24
UniRef50_A3ZMY4 Predicted PP-loop superfamily ATPase n=1 Tax=Bla...   113   5e-24
UniRef50_B8HQ99 7-cyano-7-deazaguanine synthase n=1 Tax=Cyanothe...   111   2e-23
UniRef50_A5ULR3 7-cyano-7-deazaguanine synthase n=5 Tax=Methanob...   110   3e-23
UniRef50_B3G2Q8 Putative uncharacterized protein n=1 Tax=Pseudom...   110   4e-23
UniRef50_A6DLD5 ExsB protein n=1 Tax=Lentisphaera araneosa HTCC2...   110   4e-23
UniRef50_B9L187 ExsB protein n=1 Tax=Thermomicrobium roseum DSM ...   109   6e-23
UniRef50_C0CIL2 Putative uncharacterized protein n=1 Tax=Blautia...   107   3e-22
UniRef50_A9A531 ExsB family protein n=2 Tax=Thaumarchaeota RepID...   106   6e-22
UniRef50_Q3AR02 7-cyano-7-deazaguanine synthase n=12 Tax=Bacteri...   106   8e-22
UniRef50_O29807 7-cyano-7-deazaguanine synthase n=1 Tax=Archaeog...   105   1e-21
UniRef50_B8GJL3 7-cyano-7-deazaguanine synthase n=6 Tax=Euryarch...   105   2e-21
UniRef50_Q1IHK6 7-cyano-7-deazaguanine synthase n=9 Tax=Bacteria...   104   2e-21
UniRef50_D2RGV6 ExsB protein n=2 Tax=Archaeoglobaceae RepID=D2RG...   104   3e-21
UniRef50_Q2IUE0 7-cyano-7-deazaguanine synthase 2 n=1 Tax=Rhodop...   103   4e-21
UniRef50_Q8U4N3 7-cyano-7-deazaguanine synthase n=3 Tax=Pyrococc...   103   5e-21
UniRef50_UPI0001AF28A8 succinoglycan biosynthesis regulator n=2 ...   102   7e-21
UniRef50_A6UV33 7-cyano-7-deazaguanine synthase n=12 Tax=Methano...   102   1e-20
UniRef50_C1YMB1 PreQ(0) biosynthesis protein QueC n=1 Tax=Nocard...   100   6e-20
UniRef50_B3QPB3 7-cyano-7-deazaguanine synthase n=4 Tax=cellular...   100   7e-20
UniRef50_A2STX4 7-cyano-7-deazaguanine synthase n=3 Tax=cellular...    99   1e-19
UniRef50_B3T674 Putative ExsB n=1 Tax=uncultured marine crenarch...    98   2e-19
UniRef50_B6CWJ6 ToyM n=3 Tax=Bacteria RepID=B6CWJ6_STRRM               98   2e-19
UniRef50_D1R557 Putative uncharacterized protein n=1 Tax=Parachl...    98   2e-19
UniRef50_C7ML00 ExsB protein n=9 Tax=Bacteria RepID=C7ML00_CRYCD       97   3e-19
UniRef50_UPI0001C418E0 queuosine biosynthesis protein QueC n=1 T...    97   5e-19
UniRef50_Q6WI28 ExsB protein n=1 Tax=Vibrio phage KVP40 RepID=Q6...    94   4e-18
UniRef50_Q5JHU5 7-cyano-7-deazaguanine synthase n=6 Tax=Thermoco...    94   4e-18
UniRef50_C4Z7K4 Queuosine biosynthesis protein QueC n=5 Tax=Bact...    93   8e-18
UniRef50_B2UN88 ExsB protein n=6 Tax=Bacteria RepID=B2UN88_AKKM8       92   1e-17
UniRef50_Q8UAM7 7-cyano-7-deazaguanine synthase n=71 Tax=Alphapr...    92   2e-17
UniRef50_B9M1I5 ExsB family protein n=4 Tax=Proteobacteria RepID...    90   6e-17
UniRef50_Q857D1 Gp3 n=2 Tax=unclassified Siphoviridae RepID=Q857...    89   2e-16
UniRef50_B1XTB6 ExsB protein n=1 Tax=Polynucleobacter necessariu...    88   2e-16
UniRef50_Q12WI3 7-cyano-7-deazaguanine synthase n=6 Tax=cellular...    87   4e-16
UniRef50_D2RLP8 ExsB protein n=3 Tax=Bacteria RepID=D2RLP8_ACIFE       86   8e-16
UniRef50_C8PJP9 Queuosine biosynthesis protein QueC n=1 Tax=Camp...    84   3e-15
UniRef50_D1PYH8 Transcription regulator ExsB n=5 Tax=Prevotella ...    83   6e-15
UniRef50_B2RJS2 7-cyano-7-deazaguanine synthase n=5 Tax=Bacteria...    81   2e-14
UniRef50_C0WAE7 Archaeosine biosynthesis protein queC n=3 Tax=Ba...    78   2e-13
UniRef50_A0B6A6 7-cyano-7-deazaguanine synthase n=4 Tax=Archaea ...    76   7e-13
UniRef50_Q0W7U0 Succinoglycan biosynthesis regulator n=1 Tax=unc...    76   9e-13
UniRef50_A8AAU7 ExsB protein n=1 Tax=Ignicoccus hospitalis KIN4/...    72   1e-11
UniRef50_C3XFP7 Queuosine biosynthesis protein queC n=1 Tax=Heli...    70   5e-11
UniRef50_A4J5C7 7-cyano-7-deazaguanine synthase n=4 Tax=Clostrid...    68   3e-10
UniRef50_Q6MKZ5 7-cyano-7-deazaguanine synthase n=1 Tax=Bdellovi...    67   5e-10
UniRef50_A8V022 Trans-regulatory protein ExsB n=1 Tax=Hydrogeniv...    66   7e-10
UniRef50_C9YE01 Putative uncharacterized protein n=1 Tax=Curviba...    64   5e-09
UniRef50_Q981C9 7-cyano-7-deazaguanine synthase n=13 Tax=Thermop...    62   1e-08
UniRef50_Q8TWG9 7-cyano-7-deazaguanine synthase n=1 Tax=Methanop...    58   2e-07
UniRef50_A8URJ3 Putative uncharacterized protein n=1 Tax=Hydroge...    55   1e-06
UniRef50_Q8ZVX7 7-cyano-7-deazaguanine synthase n=7 Tax=Thermopr...    54   4e-06
UniRef50_Q24ZT5 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Desulf...    54   4e-06
UniRef50_O67015 Putative uncharacterized protein n=1 Tax=Aquifex...    53   1e-05
UniRef50_Q13AQ3 ExsB n=6 Tax=Proteobacteria RepID=Q13AQ3_RHOPS         51   3e-05
UniRef50_A9U3Q1 Predicted protein n=1 Tax=Physcomitrella patens ...    51   4e-05
UniRef50_B8GP80 ExsB family protein n=1 Tax=Thioalkalivibrio sp....    50   5e-05
UniRef50_Q96ZD9 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Sulfol...    49   1e-04
UniRef50_Q8DNP8 NADPH-dependent 7-cyano-7-deazaguanine reductase...    49   2e-04
UniRef50_Q0BZ62 Putative uncharacterized protein n=1 Tax=Hyphomo...    48   2e-04
UniRef50_Q989H2 Mll6423 protein n=2 Tax=Proteobacteria RepID=Q98...    48   3e-04
UniRef50_D2L9Z8 ExsB family protein n=1 Tax=Desulfovibrio sp. FW...    47   4e-04
UniRef50_D2PHM6 ExsB family protein n=3 Tax=Sulfolobus islandicu...    47   4e-04
UniRef50_A4YH21 ExsB family protein n=1 Tax=Metallosphaera sedul...    46   8e-04
UniRef50_D2R400 ExsB family protein n=1 Tax=Pirellula staleyi DS...    45   0.002
UniRef50_D0JAY8 Bifunctional argininosuccinate synthase/acetyltr...    45   0.002
UniRef50_C9YE00 Putative uncharacterized protein (Fragment) n=2 ...    45   0.002
UniRef50_B4U7S9 ExsB family protein n=1 Tax=Hydrogenobaculum sp....    44   0.004
UniRef50_UPI00016C5260 ExsB n=1 Tax=Gemmata obscuriglobus UQM 22...    43   0.010
UniRef50_A6L476 Putative sulfate adenylate transferase, subunit ...    40   0.060

>UniRef50_B8E544 7-cyano-7-deazaguanine synthase n=198 Tax=Bacteria RepID=QUEC_SHEB2
          Length = 240

 Score =  332 bits (850), Expect = 8e-90,   Method: Compositional matrix adjust.
 Identities = 154/222 (69%), Positives = 186/222 (83%), Gaps = 1/222 (0%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AVVVFSGGQDSTTCL+QAL QYDEVH +TFDYGQRHR EI+VA+ LA +L   +HKV+D
Sbjct: 13  KAVVVFSGGQDSTTCLIQALTQYDEVHGITFDYGQRHREEIEVAKSLAKRLKITSHKVMD 72

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           VTLLNELA+S+LTRD+IPV  +E   +G+PNTFVPGRNILFLTLA IYAYQ+ AEA+ITG
Sbjct: 73  VTLLNELAISALTRDAIPV-SHELMENGLPNTFVPGRNILFLTLAGIYAYQLGAEAIITG 131

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           VCETDFSGYPDCR++FVKA+  A+  GM K ++  TPLMW++KA+TWALAD Y +LDLVR
Sbjct: 132 VCETDFSGYPDCRNDFVKAMESALVQGMDKQLKIITPLMWLNKAQTWALADKYQQLDLVR 191

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           + TLTCYNG  GDGCG C AC+LR  GL+ Y+ DK  VMA++
Sbjct: 192 HHTLTCYNGVIGDGCGDCPACHLRKRGLDEYMQDKTAVMASL 233


>UniRef50_Q1LTK2 7-cyano-7-deazaguanine synthase n=234 Tax=Bacteria RepID=QUEC_BAUCH
          Length = 238

 Score =  328 bits (842), Expect = 6e-89,   Method: Compositional matrix adjust.
 Identities = 154/231 (66%), Positives = 187/231 (80%), Gaps = 1/231 (0%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +K+ VVVFSGGQDSTTCL+QAL QYD+V+C+TFDYGQRHRAEI +AR L + LGA  H++
Sbjct: 7   IKKTVVVFSGGQDSTTCLIQALTQYDQVYCITFDYGQRHRAEICIARRLTITLGAYDHQI 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEP-EADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +D + LN LA SSLTR++I VP  E    +   +TFVPGRNI+FLTLAAIYAYQVKAE +
Sbjct: 67  IDTSALNVLAASSLTRENIIVPLAEKNHMNSSLSTFVPGRNIVFLTLAAIYAYQVKAEVI 126

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           ITGVCETDFSGYPDCR++F++ALN AV+LGMA +I F +PLMW++KAETWALADYY KLD
Sbjct: 127 ITGVCETDFSGYPDCREKFIQALNKAVNLGMAYNIHFISPLMWLNKAETWALADYYQKLD 186

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTGL 230
           LV NETLTCYNG +G GCG C +C+LRA GL  Y       M+++K KTGL
Sbjct: 187 LVCNETLTCYNGIQGKGCGFCTSCSLRARGLETYKQQPKLTMSSLKAKTGL 237


>UniRef50_Q8K986 7-cyano-7-deazaguanine synthase n=1 Tax=Buchnera aphidicola
           (Schizaphis graminum) RepID=QUEC_BUCAP
          Length = 232

 Score =  244 bits (623), Expect = 2e-63,   Method: Compositional matrix adjust.
 Identities = 104/227 (45%), Positives = 152/227 (66%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+ +++FSGGQDSTTCL+     Y E++C+TFDY Q H++EID AR ++     + H  
Sbjct: 1   MKKILIIFSGGQDSTTCLIHYTNIYKEIYCITFDYNQLHKSEIDSARFISNYFNVKKHIF 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+  L  L++SSLT + I + D  P    +P+TFVPGRNILFLTL++IYA+  +  +++
Sbjct: 61  VDLKCLKNLSISSLTDEKISILDNHPLNFSLPSTFVPGRNILFLTLSSIYAFNHQINSIV 120

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            GV E DFSGYPDCR+ F+K +N  V +GM   I F++PL+ + KAE WAL+DY+     
Sbjct: 121 LGVNEIDFSGYPDCRNAFLKKMNDVVQIGMNCKINFQSPLINLSKAEIWALSDYWNSTQF 180

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQK 227
           + N T+TCY G +G GCG C +C LR +G N + ++    M  +K+K
Sbjct: 181 ILNNTVTCYQGIQGKGCGQCQSCILRNDGFNKWKSNPSYYMKKLKEK 227


>UniRef50_A6LIS9 7-cyano-7-deazaguanine synthase n=88 Tax=Bacteria RepID=QUEC_PARD8
          Length = 224

 Score =  214 bits (546), Expect = 1e-54,   Method: Compositional matrix adjust.
 Identities = 111/215 (51%), Positives = 142/215 (66%), Gaps = 3/215 (1%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A+V FSGGQDSTTCL  A + +  V  V F YGQ+H  EI+VAR++A        ++LDV
Sbjct: 7   ALVCFSGGQDSTTCLFWAKKHFSRVEAVCFTYGQKHSLEIEVARKIAADADV-PFQLLDV 65

Query: 64  TLLNELAVS-SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +L+++L  + SLT  SI +   +PE D  PNTFVPGRN++FLT AAI A       ++TG
Sbjct: 66  SLISQLDPNCSLTNASIEMDQEKPE-DSYPNTFVPGRNMVFLTFAAILARGKGIYHLVTG 124

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           V E D+SGYPDCRD FV++LN  ++L M +     TPLM  DK+E W L+D  G  DLVR
Sbjct: 125 VSEADYSGYPDCRDTFVRSLNVTLNLAMDEQFVIHTPLMDRDKSEVWELSDELGVFDLVR 184

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADK 217
            +TLTCYNG   +GCGHC AC LR +GL  YL  K
Sbjct: 185 TQTLTCYNGVMAEGCGHCPACKLRKDGLEKYLKRK 219


>UniRef50_A6VQW6 7-cyano-7-deazaguanine synthase n=22 Tax=Pasteurellaceae
           RepID=QUEC_ACTSZ
          Length = 235

 Score =  212 bits (539), Expect = 9e-54,   Method: Compositional matrix adjust.
 Identities = 107/221 (48%), Positives = 144/221 (65%), Gaps = 8/221 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++AVV+FSGGQDSTTCL+QA++ Y  + V  VTF YGQRH  E+  A+ +A  LG +   
Sbjct: 10  RKAVVIFSGGQDSTTCLLQAIRDYGPNNVEAVTFQYGQRHAIELQKAQWIAQDLGVK-QT 68

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++D +++  +  +++  D+      + + +G+PNTFV GRN LFL  AAIYA       +
Sbjct: 69  LIDTSVIKTITTNAMMSDA----QIKQDENGMPNTFVDGRNALFLLYAAIYAKGQGITDI 124

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           ITGVCETDFSGYPDCR+ F+K++N  ++L M       TPLM++ KA+TW LAD  G LD
Sbjct: 125 ITGVCETDFSGYPDCRNVFIKSMNVTLNLAMDYQFNIRTPLMYLTKAQTWQLADELGALD 184

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
            VR  T TCY G +G GCG C +C LR  GL  YLA K  V
Sbjct: 185 YVRQYTHTCYLGVEG-GCGTCPSCLLREKGLKQYLAQKSAV 224


>UniRef50_B4E7W5 7-cyano-7-deazaguanine synthase n=73 Tax=Bacteria RepID=QUEC_BURCJ
          Length = 244

 Score =  200 bits (508), Expect = 4e-50,   Method: Compositional matrix adjust.
 Identities = 104/229 (45%), Positives = 148/229 (64%), Gaps = 22/229 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID----VARELALKLGARAHK 59
           A+V+FSGGQDS TC+  AL++Y  V  + FDYGQRHR E++    V   L  +  A + +
Sbjct: 10  ALVLFSGGQDSATCVAWALERYQTVETLGFDYGQRHRVELECREGVREALKHRFPAWSDR 69

Query: 60  V-----LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
           +     +D+++L  ++ +++TR      + E  A+G+PNTFVPGRN++F+T+AA  AY+ 
Sbjct: 70  LGDDHMIDLSVLGAISDTAMTR----TIEIETTANGLPNTFVPGRNLMFMTIAAAIAYRR 125

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADY 174
               ++ G+CETDFSGYPDCRD+ +KAL  A++LGM   +  ETPLMW+DKA+TW LA+ 
Sbjct: 126 GLRVLVGGMCETDFSGYPDCRDDTMKALQVALNLGMDTRMVLETPLMWLDKAQTWQLAEQ 185

Query: 175 YGK---LDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHYL 214
            G    ++L+R ET TCY G +      G GCG C AC LR  G   YL
Sbjct: 186 LGGDALVELIRVETHTCYVGERAELHDWGFGCGECPACKLRKRGYEAYL 234


>UniRef50_C1D6L0 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria RepID=QUEC_LARHH
          Length = 229

 Score =  183 bits (464), Expect = 5e-45,   Method: Compositional matrix adjust.
 Identities = 96/210 (45%), Positives = 133/210 (63%), Gaps = 5/210 (2%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + A+VV SGGQDSTTCL  AL ++ +V  ++FDYGQRHR E+D AR +A   G   H ++
Sbjct: 8   EHALVVLSGGQDSTTCLYWALSRFAQVSAISFDYGQRHRVELDAARTIAAMAGV-GHTII 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGI-PNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
            +   + L  ++LT D    PD  P+A+ + PNTFVPGRN++FLT AA +A+      ++
Sbjct: 67  PINTFSALGGNALT-DQQMSPDTGPDAETLLPNTFVPGRNLVFLTFAAAWAWPRGIRHIV 125

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           TGV +TD+SGYPDCR+  ++AL  A++LGM   +R   PLM++ KA+T  LA   G +  
Sbjct: 126 TGVAQTDYSGYPDCRENTLRALELAINLGMESRMRLHMPLMFLSKADTVTLARTVGAMPA 185

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGL 210
           +     TCY G     CG CAAC LRA G 
Sbjct: 186 LAFSH-TCYAG-AVPPCGQCAACVLRAKGF 213


>UniRef50_A5A3S4 BcepGomrgp35 n=1 Tax=Burkholderia phage BcepGomr RepID=A5A3S4_9CAUD
          Length = 301

 Score =  182 bits (463), Expect = 6e-45,   Method: Compositional matrix adjust.
 Identities = 99/231 (42%), Positives = 132/231 (57%), Gaps = 18/231 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K AV+VFSGGQDSTT L +A+ +   V  + F YGQ+H  E++ A ++    G    K++
Sbjct: 4   KTAVIVFSGGQDSTTILGKAIAEGYRVFPIAFTYGQKHAIELEQAAKIVDHFGLAGLKIV 63

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D++ L E+  S+L      V         +P +FVP RN  FLTLA  YA QV A+ + T
Sbjct: 64  DLSALGEVTTSALIGTDGDVNKPSDANPSLPASFVPNRNAAFLTLAHAYAQQVGADELWT 123

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GVC TD+SGYPDCRD F+ AL   +++G    I  +TPLM+IDKAET+ +A+  G LD+V
Sbjct: 124 GVCMTDYSGYPDCRDSFINALAATLNIGYDTSITIKTPLMYIDKAETFRMAELAGCLDVV 183

Query: 182 RNETLTCYNGFK------------------GDGCGHCAACNLRANGLNHYL 214
              + TCYNG +                  G GCG C AC LRA G   YL
Sbjct: 184 IEMSHTCYNGVRSNGEVPTNPSEGGGFFDWGHGCGECPACKLRAGGWREYL 234


>UniRef50_Q7M8H4 7-cyano-7-deazaguanine synthase n=2 Tax=Campylobacterales
           RepID=QUEC_WOLSU
          Length = 233

 Score =  182 bits (462), Expect = 7e-45,   Method: Compositional matrix adjust.
 Identities = 100/221 (45%), Positives = 133/221 (60%), Gaps = 10/221 (4%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A+VVFSGGQDSTTCL  A++ +  V  +TFDYGQRHR EI+ +R +A KLG +   +L+ 
Sbjct: 14  ALVVFSGGQDSTTCLGWAIKHFKSVEAITFDYGQRHRIEIEQSRLIAQKLGIKQF-ILEF 72

Query: 64  TLLNELAVSSL--TRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            L ++L  S+L  T   +    +  +   +P +FVP RN LF TLA  YA +     +IT
Sbjct: 73  DLFSKLGDSALLETSSDLKASPHRTKPH-LPASFVPNRNALFFTLAHAYAQREGLTHIIT 131

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+ E D+SGYPDCR+EF+  L   ++LG    I F  PL+  +KA+T+ALA   G LDLV
Sbjct: 132 GISEADYSGYPDCREEFITHLERTLNLGSDSSILFAYPLLHRNKAQTFALAKECGVLDLV 191

Query: 182 RNETLTCYNGFK------GDGCGHCAACNLRANGLNHYLAD 216
             E+ TCYNG +      G GC  C AC LR  G   Y A 
Sbjct: 192 LEESHTCYNGDRSHRYEWGYGCKECPACGLREKGWREYQAS 232


>UniRef50_Q0BQ87 7-cyano-7-deazaguanine synthase n=76 Tax=Bacteria RepID=QUEC_GRABC
          Length = 252

 Score =  179 bits (453), Expect = 8e-44,   Method: Compositional matrix adjust.
 Identities = 100/233 (42%), Positives = 138/233 (59%), Gaps = 27/233 (11%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV---ARELALKLGARA--- 57
           A+V+FSGGQDSTTCL  AL+++  V  + F YGQRH  E++     R++   +  R+   
Sbjct: 18  ALVLFSGGQDSTTCLAWALERFAHVETIGFTYGQRHSVEMECRGRVRQVLAHMSGRSGAD 77

Query: 58  -------HKVLDV-TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
                    VLD+   L+ +  S+LT  S P+   E    G+P +FVPGRN++FLT AA 
Sbjct: 78  WGSRLGDDHVLDLGNALHHVGQSALTGRS-PI---EIGQGGLPTSFVPGRNLIFLTYAAA 133

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
             ++     ++ G+CETD+SGYPDCRD+ +KA+  A++ GM      ETPLMW+ KAETW
Sbjct: 134 LGWRRGLRRMVGGMCETDYSGYPDCRDDTIKAMQLALNTGMEAHFVLETPLMWLTKAETW 193

Query: 170 ALADYYGK---LDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
           ALAD  G    + L+  ET TCY G +      G GCG C AC+LRA G   +
Sbjct: 194 ALADSLGGPALVALIEEETHTCYLGDRSQRHPWGYGCGECPACSLRAEGWREW 246


>UniRef50_C6XGK1 ExsB protein n=9 Tax=Bacteria RepID=C6XGK1_LIBAP
          Length = 240

 Score =  177 bits (449), Expect = 3e-43,   Method: Compositional matrix adjust.
 Identities = 94/229 (41%), Positives = 140/229 (61%), Gaps = 24/229 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV---ARELALKLGARAH-- 58
           A+++FSGGQDS+TCL  AL ++D V  ++FDYGQR++ E++     R+  ++L  +    
Sbjct: 11  ALLLFSGGQDSSTCLSWALDRFDRVETLSFDYGQRNKVELECRLCVRKKIVELMPKWKDS 70

Query: 59  ----KVLDVTLLNELAVSSLTRD-SIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
                +L + +L +++ SSLT++ ++ + D     + +PNTFVPGRNI+FL  AA  AY+
Sbjct: 71  LGEDHILPLAILGDISHSSLTKNVAMKIQD-----NNLPNTFVPGRNIIFLVFAATLAYR 125

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           +    ++ GVCETD+SGYPDCR + ++A+  A++LGM   +   TPLMW+ K ETW LA 
Sbjct: 126 LGITNIVIGVCETDYSGYPDCRHDTIRAIETAINLGMESHVTVHTPLMWLKKYETWKLAQ 185

Query: 174 YYGKLDLVR---NETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
             G  DLV     E+ TCY G +      G GC  C AC LR  G   +
Sbjct: 186 DIGGQDLVNLILEESHTCYLGKRDKRYEWGYGCNSCPACYLRQKGWMEF 234


>UniRef50_Q7NCE2 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria RepID=QUEC_GLOVI
          Length = 234

 Score =  172 bits (435), Expect = 1e-41,   Method: Compositional matrix adjust.
 Identities = 103/226 (45%), Positives = 135/226 (59%), Gaps = 11/226 (4%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+A+VVFSGGQDSTTC   A ++YDEVH VTF+Y QRH  E++ AR +   LG   H+ 
Sbjct: 1   MKKALVVFSGGQDSTTCAALACREYDEVHAVTFEYNQRHAIELESARAVGQALGLTGHEF 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEAD---GIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           + +  L +   S L  D+ P+  Y   A+   G+  TFVPGRNILFLTLAA  A+ +   
Sbjct: 61  IRLGPLLK-GTSPLVSDA-PLGQYASAAELPAGVEPTFVPGRNILFLTLAANRAFCLGTG 118

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD---IRFETPLMWIDKAETWALA-D 173
            ++ GVCE DF+GY DCR  FV+A+  A+  G+  D   IR  TPLM + KAET  L+ +
Sbjct: 119 DIVIGVCEADFAGYWDCRQVFVEAMARALGEGIYGDANAIRIHTPLMRLTKAETVKLSVE 178

Query: 174 YYG-KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
             G + + V   + TCY G +G GCG C AC LR  G        P
Sbjct: 179 VLGERFEEVLALSHTCYAGVRG-GCGRCHACILRDRGFREAGVPDP 223


>UniRef50_Q1CTN3 7-cyano-7-deazaguanine synthase n=12 Tax=Helicobacter
           RepID=QUEC_HELPH
          Length = 228

 Score =  168 bits (425), Expect = 1e-40,   Method: Compositional matrix adjust.
 Identities = 85/216 (39%), Positives = 133/216 (61%), Gaps = 8/216 (3%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           VV FSGGQDSTT  V A +++ +V+ V FDY Q+H  E++ A+++A  L    ++++ + 
Sbjct: 7   VVSFSGGQDSTTLAVWAKKRFKKVYLVGFDYAQKHSVELECAQKIA-SLLQLPYEIISLD 65

Query: 65  LLNELAVSSLTRDSIPVPDYEP-EADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            L  +  S+L ++S  +  +   +   +PN+FVP RN +F+TL   YA ++ A  +  GV
Sbjct: 66  FLENITRSALFKNSNDLMGHSHVQNKDLPNSFVPNRNAIFITLLHSYAQKIGASNIALGV 125

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
            + DFSGYPDC+++F+K++ HA++LG    I+  TPLM+++KA+ + +A   G LDLV  
Sbjct: 126 SQADFSGYPDCKEDFIKSIEHALNLGSNTAIKILTPLMFLNKAQEFQMAKDLGVLDLVIK 185

Query: 184 ETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
           ET TCY G +      G GC  C AC LR  G   +
Sbjct: 186 ETHTCYQGERKILHAYGYGCNECPACQLRKKGYEEF 221


>UniRef50_D0MDN1 ExsB protein n=1 Tax=Rhodothermus marinus DSM 4252
           RepID=D0MDN1_RHOM4
          Length = 238

 Score =  162 bits (411), Expect = 6e-39,   Method: Compositional matrix adjust.
 Identities = 96/212 (45%), Positives = 125/212 (58%), Gaps = 9/212 (4%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A+V+ SGGQDSTTCL  AL  + +V  + F YGQ+H  E++ AR++A + G     VLD+
Sbjct: 19  ALVLLSGGQDSTTCLYWALHYFPKVEALGFHYGQKHAVELEQARKIADRAGV-PFTVLDL 77

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             L  L  S+LT     V    P A  +P  FVPGRN LFLTLAA Y +      ++ G+
Sbjct: 78  RGL--LRGSALTEHEQDVSAAHPLAPHLPAAFVPGRNALFLTLAASYGFTRGIHDLVGGM 135

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
           C+TD+SGYPDCR EF+ A+  A+SL +   IR  TPLM + KAETW LA   G LD++  
Sbjct: 136 CQTDYSGYPDCRREFIDAMERALSLALDTPIRIHTPLMHLTKAETWRLARELGILDVIVE 195

Query: 184 ETLTCYNGFKGD----GCGHC--AACNLRANG 209
            T T YNG + +    G G     A  LRA G
Sbjct: 196 LTHTDYNGDRSERHPWGYGRLDNPASILRARG 227


>UniRef50_B2KDX5 ExsB protein n=1 Tax=Elusimicrobium minutum Pei191
           RepID=B2KDX5_ELUMP
          Length = 484

 Score =  148 bits (373), Expect = 2e-34,   Method: Compositional matrix adjust.
 Identities = 87/212 (41%), Positives = 124/212 (58%), Gaps = 8/212 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K+AVV+FSGG DST CL  AL++  E + ++  YGQ+H  EI+VA+ LA + G   HK +
Sbjct: 269 KKAVVLFSGGLDSTVCLYWALKEGYECYALSVAYGQKHLKEIEVAQRLAKQAGV-THKHI 327

Query: 62  DVTLLNELAVSSLTRDS-IPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++ L      +SL   + IP    E     IP+T+VP RN++F++L A +A  + AEAV+
Sbjct: 328 EINLPWLAEATSLVGGAEIPATPMEQIGKSIPSTYVPARNLIFVSLGASWADSIGAEAVV 387

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE--TPLMWIDKAETWALADYYGKL 178
            G    D+SGYPDC  +F K L+ AV +G     +FE  TP++ ++KAE   L     KL
Sbjct: 388 LGPNAVDYSGYPDCTPQFYKPLSEAVRVGTRLGKKFEILTPIIKLNKAEIVKLGR---KL 444

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
                +T TCYNG +   CG C +C LR  G 
Sbjct: 445 GAPLEQTWTCYNGGE-KPCGVCESCKLRRKGF 475


>UniRef50_Q1QCP3 7-cyano-7-deazaguanine synthase n=122 Tax=Bacteria RepID=QUEC_PSYCK
          Length = 259

 Score =  142 bits (359), Expect = 8e-33,   Method: Compositional matrix adjust.
 Identities = 86/235 (36%), Positives = 121/235 (51%), Gaps = 22/235 (9%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + AVV+ SGG DS TCL  A  +Y  V  V+FDYGQRH +E+  A+ +A   G   H+++
Sbjct: 26  QNAVVLLSGGLDSVTCLYWAKARYASVTAVSFDYGQRHNSELVAAKAIAETAGVN-HRII 84

Query: 62  DVTLLNELAVSSLTRDSIPVPDY-----------EPEADGIPNTFVPGRNILFLTLAAIY 110
           D+ +  +L  SSLT  S+ VPD            E + D IPNT+VP RN +FL+ A   
Sbjct: 85  DIDIA-QLGGSSLTDHSMIVPDGDTDKFPDKKRDEIDNDAIPNTYVPARNTIFLSYALAV 143

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLG-----MAKDIRFETPLMWIDK 165
           A    +  ++ GV   D+SGYPDCR E++ A  H  +L          +  +TPL  + K
Sbjct: 144 AEVTDSNHIVIGVSSVDYSGYPDCRPEYIAAFQHMANLATKAGVTGHHLSIQTPLQQLSK 203

Query: 166 AETWALADYYGKLDLVRNETLTCYNG-FKGDGCGHCAACNLRANGLNHYLADKPT 219
           A+T  L      L +   +T++CY     G  CG C +C LR  G        PT
Sbjct: 204 AKTIELG---LSLGVDYGQTISCYQADANGLACGVCDSCALRRQGFAQAGVADPT 255


>UniRef50_A7HHA2 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria RepID=QUEC_ANADF
          Length = 233

 Score =  141 bits (356), Expect = 2e-32,   Method: Compositional matrix adjust.
 Identities = 90/227 (39%), Positives = 120/227 (52%), Gaps = 12/227 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           KRAVV+ SGG DSTTCL  A +   E HC++ DYGQRH+ E+  AR L+  LGA  H+V+
Sbjct: 7   KRAVVLLSGGLDSTTCLAVARRDGFEAHCLSVDYGQRHKGELARARRLSRALGAADHRVV 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            V  L+    S+LT  +I VP        A  IP T+VP RN + L LA  +A  + AE 
Sbjct: 67  KVD-LSAFGGSALTDRAIAVPKGRSGRRRAAEIPVTYVPARNTVLLALALAHAETLGAED 125

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALAD 173
           V  GV   D+SGYPDCR  F++A     ++        + +R   PL+ + KA    L  
Sbjct: 126 VYVGVNAIDYSGYPDCRPAFLRAFERLATVATKAGVEGRPLRIHAPLLRLTKAGIVRLGT 185

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
             G   +    TL+CY+  +G  CG C AC LR  G        PT+
Sbjct: 186 SLG---VPYRVTLSCYDPVRGRACGACDACVLRRKGFAEAGIPDPTL 229


>UniRef50_A0LJR5 7-cyano-7-deazaguanine synthase n=4 Tax=Bacteria RepID=QUEC_SYNFM
          Length = 234

 Score =  135 bits (339), Expect = 1e-30,   Method: Compositional matrix adjust.
 Identities = 87/228 (38%), Positives = 125/228 (54%), Gaps = 15/228 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           ++AVV+FSGG DSTTCL  A  +  E + ++F Y QRH  E++ AR  A  LGAR H VL
Sbjct: 7   RKAVVLFSGGIDSTTCLAVAKAEGFEAYALSFFYHQRHGVEVEAARRSAPALGAREHLVL 66

Query: 62  DVTLLNELAVSSLT-RDSIP--VPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           ++  L+ +  S+LT  + +P  VP  E  +D IP T+VP RN +FL+ A  +A  + A  
Sbjct: 67  ELP-LDRIGGSALTGHEEVPKDVP-VEKMSDRIPATYVPARNTIFLSFALAWAEVLGAAD 124

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD------IRFETPLMWIDKAETWALA 172
           +  GV   D+SGYPDCR E++ A     +L + +       I+  TPL+ + K E     
Sbjct: 125 IFIGVNALDYSGYPDCRPEYIAAFERMANLAIRETVEGRMRIKVHTPLIRLTKGEIIKRG 184

Query: 173 DYYGKLDLVRNETLTCYN-GFKGDGCGHCAACNLRANGLNHYLADKPT 219
               +L L  + T +CY+   +G  CGHC +C LR  G        PT
Sbjct: 185 L---ELGLDYSLTHSCYDPDARGLACGHCDSCILRKKGFMEAGIPDPT 229


>UniRef50_B2I4Y4 7-cyano-7-deazaguanine synthase n=16 Tax=Bacteria RepID=QUEC_XYLF2
          Length = 230

 Score =  133 bits (334), Expect = 5e-30,   Method: Compositional matrix adjust.
 Identities = 87/225 (38%), Positives = 117/225 (52%), Gaps = 13/225 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AV++ SGG DS      A  Q   VH ++  YGQRH +E+D A  +A  L   AHKV
Sbjct: 1   MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKV 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +DV  L  +  S+LT D I +PD   E  GIP T+VP RN + L+LA  +A  + A  + 
Sbjct: 61  VDVD-LRSIGGSALT-DDIEIPDAGGE--GIPVTYVPARNTIMLSLALGWAEVIGAADIF 116

Query: 121 TGVCETDFSGYPDCRDEFVKAL----NHAVSLGM-AKDIRFETPLMWIDKAETWALADYY 175
            GV   D+SGYPDCR +F+ A     N A  +G+    +    PL ++ KAE       +
Sbjct: 117 CGVNAVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAPLQFLSKAEIVHEGLLH 176

Query: 176 GKLDLVRNETLTCYNG-FKGDGCGHCAACNLRANGLNHYLADKPT 219
           G +D     T++CY     G  CG C AC LR  G        PT
Sbjct: 177 G-VDF--GLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPT 218


>UniRef50_UPI0001C171C7 ExsB n=2 Tax=Nostocaceae RepID=UPI0001C171C7
          Length = 282

 Score =  132 bits (333), Expect = 7e-30,   Method: Compositional matrix adjust.
 Identities = 81/232 (34%), Positives = 118/232 (50%), Gaps = 13/232 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + +AV++ SGG DS+T L QA     E + ++FDY QRHR E+  A  +A  L    H++
Sbjct: 56  IMKAVILLSGGLDSSTVLYQARADGYECYVISFDYHQRHRRELQSAISIAQSLEVVEHQL 115

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +   L      S+LT +SIPVP   P    +  IP T+VP RN +FL+ A  YA  ++AE
Sbjct: 116 VTFDL-RSWGGSALTDNSIPVPQNRPLDQMSGSIPITYVPARNTIFLSFALAYAEAIRAE 174

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALA 172
            V  GV   D+SGYPDCR +++ A+     LG       K I    PL+ + K++   L 
Sbjct: 175 RVYIGVNALDYSGYPDCRPDYIHAMQEVFRLGTKQGREGKRIDIVAPLINLTKSQIIQLG 234

Query: 173 DYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           +  G   +    T +CY+G     CG C +C LR    +    + P     M
Sbjct: 235 NLLG---VPWELTWSCYSG-DQQPCGVCDSCQLRWAAFHELGLEDPVKQQGM 282


>UniRef50_A8GPK3 7-cyano-7-deazaguanine synthase n=18 Tax=Proteobacteria
           RepID=QUEC_RICAH
          Length = 227

 Score =  132 bits (332), Expect = 8e-30,   Method: Compositional matrix adjust.
 Identities = 80/221 (36%), Positives = 123/221 (55%), Gaps = 17/221 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AV++ SGG DS T L  A +   E+H ++F+YGQR+ AE+   +EL  +   + HK+
Sbjct: 1   MKKAVMLVSGGADSATVLAMAREIGYEIHSMSFNYGQRNNAELRKVKELIKEYNVKQHKI 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYE-----PEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
           +D+  L     S+LT D+I VP Y      PE   +P T+VP RN +FL+ A  YA  + 
Sbjct: 61  VDID-LRAFGGSALTDDNIDVPHYHDVNAVPE--DVPVTYVPSRNTIFLSYALGYAEVIG 117

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKAL----NHAVSLGM-AKDIRFETPLMWIDKAETWA 170
           A+ +  GV  +D + YPDCR E++K+     N A S+G+  + I   TPL+ + K +   
Sbjct: 118 AKDIFIGVHTSDSANYPDCRPEYIKSFEKMANLATSIGIQGQKITIHTPLIDMTKEQIIR 177

Query: 171 LADYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGL 210
                 KL +    T++CY   + D  CG+C AC +R +  
Sbjct: 178 TG---LKLGVDYKNTISCYEPTEDDSSCGNCLACMIRLDAF 215


>UniRef50_D1R584 Putative uncharacterized protein n=1 Tax=Parachlamydia
           acanthamoebae str. Hall's coccus RepID=D1R584_9CHLA
          Length = 231

 Score =  132 bits (332), Expect = 1e-29,   Method: Compositional matrix adjust.
 Identities = 80/224 (35%), Positives = 123/224 (54%), Gaps = 11/224 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+AVV+ SGG DS+ CL  A++ +  + V  ++F Y QRH +E++ A+++        H 
Sbjct: 5   KKAVVIHSGGMDSSICLALAIRTFSVENVLSLSFIYDQRHSSELEQAKKICQSWNVD-HI 63

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +L +  L E+  ++LT  S+P+     EA   PNT V GRN L   L AI+A+++ A  +
Sbjct: 64  ILSIPCLKEITTNALTDTSLPIQCGNGEA---PNTLVVGRNGLMAQLGAIHAHELGAHCI 120

Query: 120 ITGV--CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRF--ETPLMWIDKAETWALADYY 175
             G+   E + SGY DC   ++  L   +      D  F  ETPL+ + K +T  LA   
Sbjct: 121 YMGIIGVEGNHSGYRDCSRAYMD-LKQTLLRLDLDDHLFTIETPLVDMSKKKTLELAQEM 179

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
           G L+ + NET+TCY G +  GC  C AC LR  G+  + A+ P+
Sbjct: 180 GVLEFLLNETITCYEGLEKLGCQKCPACILRNEGIRQFSAENPS 223


>UniRef50_UPI00016988F1 exsB protein n=1 Tax=Endoriftia persephone 'Hot96_1+Hot96_2'
           RepID=UPI00016988F1
          Length = 213

 Score =  130 bits (327), Expect = 4e-29,   Method: Compositional matrix adjust.
 Identities = 72/163 (44%), Positives = 99/163 (60%), Gaps = 8/163 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           KRAVV+ SGG DS T L  A QQ  E H ++  YGQRH AE+D AR++A  LGA  HK++
Sbjct: 5   KRAVVLLSGGLDSATVLAMARQQGFECHALSMLYGQRHAAELDAARQVAQALGAVDHKLI 64

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++  L+++  S+LT + I VP    E  GIP T+VP RN +FL+LA  +A  ++A+ +  
Sbjct: 65  NIG-LDDIGGSALTDEQIAVPKTPSE--GIPPTYVPARNTVFLSLALGWAEVLEAQDIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETP 159
           GV   D+SGYPDCR EF+ A     +L        + I   TP
Sbjct: 122 GVNAVDYSGYPDCRPEFIVAFEQLANLATKAGVEGRRIHIHTP 164


>UniRef50_A4XFR8 7-cyano-7-deazaguanine synthase n=2 Tax=cellular organisms
           RepID=QUEC_CALS8
          Length = 225

 Score =  127 bits (320), Expect = 2e-28,   Method: Compositional matrix adjust.
 Identities = 73/209 (34%), Positives = 112/209 (53%), Gaps = 5/209 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVVV SGG DSTT L     Q  E + ++F YGQ+H  E++ A++   +L    HK++D
Sbjct: 2   RAVVVLSGGMDSTTLLYDVKNQGYETYTISFLYGQKHSKELEFAKK-TCELLKVPHKIVD 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++   +LA S+LT+ S  VP+     + +  T VP RN++FL+LA  YA  +KA+ +  G
Sbjct: 61  ISFFADLAPSALTKSSWSVPEGYYTDESMKQTVVPNRNMVFLSLATSYAISLKAQKLFYG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+A+  ++ L   + +  E P + + K +   +     KL +  
Sbjct: 121 AHAGDHPIYPDCRKEFVEAMKRSILLCDYQIVELEAPYVDLKKEDILKIG---LKLGVDY 177

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLN 211
           + T +CY G +   CG C  C  R     
Sbjct: 178 SLTWSCYKGGE-KACGRCGTCTERIEAFR 205


>UniRef50_B0TFM0 Exsb protein, putative n=1 Tax=Heliobacterium modesticaldum Ice1
           RepID=B0TFM0_HELMI
          Length = 247

 Score =  127 bits (319), Expect = 3e-28,   Method: Compositional matrix adjust.
 Identities = 86/236 (36%), Positives = 121/236 (51%), Gaps = 33/236 (13%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +RAVV+ SGG DSTTC+  A  + +++  +TF Y QRH  EI+ A+ +A   GA  H+++
Sbjct: 6   ERAVVILSGGLDSTTCMALADCRGEDIFALTFAYNQRHIREIEAAQAVACHYGAH-HQII 64

Query: 62  DVTLLNELAVSSLT----RDSIPVP---------------DYEPEA--DGIPNTFVPGRN 100
           D+  +   A S+LT    R  +  P               +  P A    IP T+VP RN
Sbjct: 65  DLGAV--FAASALTSTEIRPEVAAPLGGSAVAKMEAMGCAEKNPVAIATAIPATYVPARN 122

Query: 101 ILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIR 155
           ILFL  A  +A +V A  + TGV   D  GYPDCR EF+ A    + +G       + + 
Sbjct: 123 ILFLAYALSFAEKVGARRIYTGVNALDNPGYPDCRPEFIAAFQRMIDVGTKAGTEGRSVE 182

Query: 156 FETPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLN 211
             TPL+ ++KA+   LA     LD   + TLTCY G +   CG C +C LR  G  
Sbjct: 183 VFTPLIEMNKADIIRLAL---DLDAPLHLTLTCYYGER-PACGECNSCRLRLEGFR 234


>UniRef50_Q1QVW9 7-cyano-7-deazaguanine synthase n=23 Tax=Proteobacteria
           RepID=QUEC_CHRSD
          Length = 251

 Score =  126 bits (316), Expect = 8e-28,   Method: Compositional matrix adjust.
 Identities = 77/205 (37%), Positives = 115/205 (56%), Gaps = 6/205 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AVV++SGG DS T L +AL+   EVH ++F YGQRH  E++ A ++  +LG  AH+V+D
Sbjct: 30  KAVVIYSGGMDSYTVLHRALRAGFEVHALSFHYGQRHSRELETAHDVCQRLGI-AHQVVD 88

Query: 63  VTLLNEL-AVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +  ++ L   S+LT  +  +PD + +AD +  T VP RN++ L+LA  +A  + A     
Sbjct: 89  IRAIHGLIGNSALTDATQTMPDGDYDADNMAATVVPNRNMILLSLAIGHAVNIGANVCFY 148

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G    D   YPDCR EFV+ +N   ++     +R   P +   K E   LAD    + L 
Sbjct: 149 GAHGGDHVLYPDCRPEFVERMNDVAAIADFTPVRIAAPYLHASKEEI--LADGL-AMGLD 205

Query: 182 RNETLTCYNGFKGDGCGHCAACNLR 206
             +T TCY G +   CGHC +C  R
Sbjct: 206 YAQTWTCYLGAE-RSCGHCGSCRER 229


>UniRef50_Q2N612 7-cyano-7-deazaguanine synthase n=56 Tax=Bacteria RepID=QUEC_ERYLH
          Length = 239

 Score =  124 bits (310), Expect = 3e-27,   Method: Compositional matrix adjust.
 Identities = 85/234 (36%), Positives = 118/234 (50%), Gaps = 21/234 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K A ++ SGG DS      A +Q   VH +T DYGQRH+ E+  AR +A K+GA  H  +
Sbjct: 7   KAAAILLSGGLDSMVTAAIAQEQGYAVHALTIDYGQRHKVELQSARLIAAKIGAERHVEI 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +  L   L  S+LT D I VP      D IP T+VP RN++FL+L   +A    A  +  
Sbjct: 67  EADL-RALGGSALT-DDIDVPKCGVGND-IPVTYVPARNLVFLSLTTAFAEASGARDIFI 123

Query: 122 GVCETDFSGYPDCRDEFVKAL----NHAVSLGMAKDIRFE--TPLMWIDKAETWALADYY 175
           GV   D+SGYPDCR EF+++     N A   G+ +   F+   PL  + KA+   +A   
Sbjct: 124 GVNALDYSGYPDCRPEFIESFAVTANLATKAGVEESAPFKIHAPLQHMTKAD---IAREC 180

Query: 176 GKLDLVRNETLTCYNGFKG-DGCGHCAACNLRANGLNH--------YLADKPTV 220
            +L L    + +CY+   G   CG C +C LR  G           Y AD P +
Sbjct: 181 ARLGLESAWSWSCYDPLPGLKPCGACDSCRLRRKGFAEAGLADGLDYPADAPPI 234


>UniRef50_B0S9S9 7-cyano-7-deazaguanine synthase n=13 Tax=Bacteria RepID=QUEC_LEPBA
          Length = 248

 Score =  123 bits (309), Expect = 4e-27,   Method: Compositional matrix adjust.
 Identities = 81/229 (35%), Positives = 115/229 (50%), Gaps = 26/229 (11%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD-------EVHCVTFDYGQRHRAEIDVARELALKLG 54
           K A+V+ SGG DSTTCL  A + +         +  ++FDY Q+H+ E+  ++++A  LG
Sbjct: 16  KGAIVLLSGGLDSTTCLYLAAKDFGYPKNKKLPLLALSFDYSQKHKIELTKSKKIAKTLG 75

Query: 55  ARAHKVLDVTLLNELAVSSLTRDSIPVPD-----YEPEADGIPNTFVPGRNILFLTLAAI 109
            +   V+          SSLT   I V       +  +   IPNT+VPGRNILFL+ A  
Sbjct: 76  IK--HVIQKLDPGFFLGSSLTESKIKVRKNAKSLFSGDETEIPNTYVPGRNILFLSFALS 133

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAK-------DIRFETPLMW 162
            A     +++  GV   D+SGYPDCR EF+ +     +LG  K        I+ +TPL+ 
Sbjct: 134 LAEGHGYDSIYIGVNALDYSGYPDCRPEFIDSFQKMANLGTKKGVSGTGDSIQIKTPLLH 193

Query: 163 IDKAETWALADYYG-KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
           + K E   L    G  L L    T +CY+   G  CG C +C LRA G 
Sbjct: 194 LGKKEIIELGMSVGAPLGL----THSCYDPVSGKPCGKCDSCILRAKGF 238


>UniRef50_C8R2H0 ExsB protein n=1 Tax=Desulfurivibrio alkaliphilus AHT2
           RepID=C8R2H0_9DELT
          Length = 258

 Score =  123 bits (308), Expect = 6e-27,   Method: Compositional matrix adjust.
 Identities = 90/245 (36%), Positives = 126/245 (51%), Gaps = 25/245 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVV+ SGG DSTT L  A       HC++F YGQRH  E+++AR  A   GA  H +L++
Sbjct: 18  AVVLLSGGLDSTTVLALAKAAGYRCHCLSFRYGQRHTQELELARANARHWGAARHLILNI 77

Query: 64  TLLNELAVSSLTRDSIPVPD-----------YEPEADG----IPNTFVPGRNILFLTLAA 108
            L + +  S+LT D + VP             E +A G    +P T+VPGRN +FL  AA
Sbjct: 78  EL-DAIGGSALTGDELEVPKDNLSPLDSPDGAETDAPGMAGEVPVTYVPGRNTIFLAYAA 136

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWI 163
            +A  + A  +  GV   DFSGYPDCR ++++AL  A++LG       +  R + PL+ +
Sbjct: 137 SWAEVLGARDIFIGVNAVDFSGYPDCRPDYLEALAAALNLGSQCGREGRPFRLQAPLLHL 196

Query: 164 DKAETWALADYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVMA 222
            KAE  A     G +D  R  T +CY+  +    CG C AC LR  G        PT   
Sbjct: 197 SKAEIIAKGLALG-VDYAR--THSCYDPDQQHRACGRCDACILRRRGFAAAGCPDPTTYQ 253

Query: 223 AMKQK 227
             + +
Sbjct: 254 PARSE 258


>UniRef50_A8EWF5 7-cyano-7-deazaguanine synthase n=27 Tax=Epsilonproteobacteria
           RepID=QUEC_ARCB4
          Length = 228

 Score =  122 bits (306), Expect = 1e-26,   Method: Compositional matrix adjust.
 Identities = 75/214 (35%), Positives = 117/214 (54%), Gaps = 8/214 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKV 60
           K+A+ + SGG DST     A +   E+  V F+YGQR    E+   R++  +L  ++   
Sbjct: 7   KKAICILSGGMDSTLSSYIAKKDGFEIIAVHFNYGQRTQNRELKAFRDICNELDIKSKYE 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+    ++  ++LT  SI VP    EA G+P T+VP RN +FL + A  A +  A A+ 
Sbjct: 67  IDIPFFTQIGANALTDMSIDVPTGGLEA-GVPITYVPFRNGIFLAITAAIAEKEGATAMY 125

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE--TPLMWIDKAETWALADYYGKL 178
            GV + D SGYPDC +EF++ +  A++ G  +D + E  TPL+ + KA+    A    KL
Sbjct: 126 IGVVQEDSSGYPDCTEEFIQDMKKAINQGTKEDTKIEIITPLVHLSKAQIVQEAI---KL 182

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNH 212
           ++    T +CY   + + CG C +C LR NG   
Sbjct: 183 NVPLELTWSCYKE-EEEACGVCDSCRLRLNGFEQ 215


>UniRef50_C0QST3 7-cyano-7-deazaguanine synthase n=7 Tax=Aquificales
           RepID=QUEC_PERMH
          Length = 227

 Score =  119 bits (299), Expect = 6e-26,   Method: Compositional matrix adjust.
 Identities = 79/221 (35%), Positives = 117/221 (52%), Gaps = 14/221 (6%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           +++ SGG DS   L  A +++D+V+ V+FDYGQ+HR E+D A++L  + G   H + +V 
Sbjct: 8   IILVSGGMDSAALLWLAKKEFDKVYAVSFDYGQKHRVELDFAKKLTEEAGVEEHFIAEVP 67

Query: 65  LLNELAVSSLTRDSIPVPDYE-PEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            L  +  S+LT  S+ +P  E PE  G P T VP RN+ FL++AA +A   + E +  GV
Sbjct: 68  HLKGIKGSALTDRSVEIPSEEYPE--GPPVTTVPMRNLNFLSIAASFADIYEIENIGIGV 125

Query: 124 CETDFSGYPDCRDEFVKALNHAV---SLGMAKD---IRFETPLMWIDKAETWALADYYGK 177
              D S YPDCR EFV A   A+   S+ +AK    I   TP + + K +   +     +
Sbjct: 126 HSVD-SPYPDCRAEFVSAAEAAINASSVMVAKKKNRITVYTPFLGMTKTDVLRIGL---E 181

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
           L +   +T +CY G     CG CA C  R         + P
Sbjct: 182 LGVPYEKTYSCYRG-TIPPCGECATCRQRIEAFRSVGLEDP 221


>UniRef50_Q8YM45 7-cyano-7-deazaguanine synthase n=14 Tax=cellular organisms
           RepID=QUEC_ANASP
          Length = 229

 Score =  117 bits (294), Expect = 2e-25,   Method: Compositional matrix adjust.
 Identities = 82/232 (35%), Positives = 120/232 (51%), Gaps = 15/232 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV++ SGG DS+T L QA     E + ++FDY QRHR E+  A  +A   G   H+V++
Sbjct: 2   KAVILLSGGLDSSTILYQAKADGCECYSISFDYQQRHRRELHSAFLVAQTAGIVQHQVIN 61

Query: 63  VTLLNELAVSSLTRDSIPVP---DYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
             L      S+LT D+I +P     +  +  IP T+VP RN +FL+ A  YA  + A+ V
Sbjct: 62  FDL-RLWGGSALTDDNIDLPQERSLDAMSQNIPVTYVPARNTIFLSFALAYAEAIAAQRV 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADY 174
             GV   D+SGYPDCR ++++A+     LG       + I    PL+ + K E   L + 
Sbjct: 121 YIGVNALDYSGYPDCRPDYIEAMQEVFRLGTKQGREGQPINIVAPLINLKKTEIIQLGN- 179

Query: 175 YGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVMAAMK 225
             +L +  N T +CY G  GD  CG C +C LR         + P   A +K
Sbjct: 180 --QLGVPWNLTWSCYAG--GDVACGVCDSCRLRLAAFAELGLEDPLPYAYLK 227


>UniRef50_Q6ARX9 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria RepID=QUEC_DESPS
          Length = 219

 Score =  116 bits (290), Expect = 7e-25,   Method: Compositional matrix adjust.
 Identities = 75/217 (34%), Positives = 106/217 (48%), Gaps = 14/217 (6%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLG-----A 55
           M  +++V SGG DS   L Q L++      ++FDYG +H       REL + +G     A
Sbjct: 1   MVDSIIVLSGGMDSAVLLAQTLKEGKTAQAISFDYGSKHND-----RELPMAVGLCEEFA 55

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
             H+++ +  + EL  SSL      +PD     + + +T VP RN + L +AA YA  V+
Sbjct: 56  IKHQIIKLPFIGELFSSSLLTGGEEIPDGAYGQENMKSTVVPFRNGIMLAIAAGYAESVQ 115

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
           A  V+ G    D   YPDCR EF  A   AV+LG    +    P   + K +   + D  
Sbjct: 116 AGEVLLGSHSGDHHIYPDCRPEFNNAFAEAVNLGTDGQVAIRFPFSEMTKRD---IGDLG 172

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNH 212
             LDL   +T TCY G +   CG CAAC+ R   L H
Sbjct: 173 RTLDLDFAKTWTCYKGGELH-CGVCAACDERKEALRH 208


>UniRef50_B9MMB0 7-cyano-7-deazaguanine synthase n=8 Tax=Bacteria RepID=QUEC_ANATD
          Length = 221

 Score =  116 bits (290), Expect = 7e-25,   Method: Compositional matrix adjust.
 Identities = 73/211 (34%), Positives = 108/211 (51%), Gaps = 9/211 (4%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVVV SGG DSTT L     Q  EV  ++F YGQ+H  E++ A++    L +  +K++D
Sbjct: 2   RAVVVLSGGMDSTTLLYDVKSQGYEVFAISFLYGQKHSKELEFAKK-TCSLLSIPYKIVD 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++   ELA S+LT     VP+       +  T VP RN++ L+++A YA  + A+ +  G
Sbjct: 61  ISFFAELAPSALTTPDWNVPEGYYTDSTMKQTVVPNRNMVLLSISAAYAISLGAKKLFYG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+A+ +A+ L     +  E P + + K +          L+L  
Sbjct: 121 AHAGDHPIYPDCRKEFVEAMKNALYLADYIGLELEAPYVDMKKEDILRRG-----LELGV 175

Query: 183 NETLT--CYNGFKGDGCGHCAACNLRANGLN 211
           N +LT  CY G +   CG C  C  R     
Sbjct: 176 NYSLTWSCYKGGQ-KACGRCGTCTERIEAFK 205


>UniRef50_B9Z9V1 ExsB protein n=2 Tax=Halobacteriaceae RepID=B9Z9V1_NATMA
          Length = 251

 Score =  116 bits (290), Expect = 7e-25,   Method: Compositional matrix adjust.
 Identities = 73/208 (35%), Positives = 106/208 (50%), Gaps = 7/208 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKV 60
            RAVV+ SGG DS T   +A  Q  +++ +   YGQR    E++ AR+LA +  A     
Sbjct: 23  NRAVVLLSGGMDSATAAAEARSQGYDIYALHTSYGQRTETRELECARQLADEFEANDFLQ 82

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++   L+ +  SSLT D + V D + ++D IP+++VP RN   L +A  YA     EAV 
Sbjct: 83  IETGHLSAIGASSLTDDEMAVADADMDSDEIPSSYVPFRNANLLAMAVSYAEANDCEAVF 142

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKL 178
            G    DFSGYPDCR EF +A      +G     +I  E P +   K +   +A +  +L
Sbjct: 143 IGAHSEDFSGYPDCRPEFFEAFERVADVGTKPETEISIEAPFVEWSKTD---IAAHGTEL 199

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLR 206
            +    T +CY   +   CG C AC  R
Sbjct: 200 GVPYEHTWSCYRDDQP-ACGTCDACAYR 226


>UniRef50_Q55468 7-cyano-7-deazaguanine synthase n=43 Tax=Bacteria RepID=QUEC_SYNY3
          Length = 232

 Score =  114 bits (284), Expect = 4e-24,   Method: Compositional matrix adjust.
 Identities = 73/214 (34%), Positives = 109/214 (50%), Gaps = 10/214 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K AVV+ SGG DS T    A ++   V  ++F+YGQRH  E+  A ++   LG   H  +
Sbjct: 3   KTAVVLLSGGLDSATVAAIAKREGYRVIALSFNYGQRHDRELRAAADIVQALGIPEHFSI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++ L  +   SSLT     +P    E D IP+T+VPGRN +F+ L    A    AEAV  
Sbjct: 63  NLDL-AQWGGSSLTDRQQTLPQTGVEPDIIPSTYVPGRNTVFIALGLSLAEAKGAEAVFL 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADYYG 176
           G+   D+SGYPDCR E++       +L        + I+   PL+ + K +   +     
Sbjct: 122 GINAIDYSGYPDCRPEYLATYQQLAALSSKVGVEGRPIQLLAPLIELSKVD---IVHKAL 178

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
           +L +   +T +CY G + + CG C +C LR   L
Sbjct: 179 ELGVPIAQTWSCYAGGE-EPCGRCDSCRLRDQAL 211


>UniRef50_A3ZMY4 Predicted PP-loop superfamily ATPase n=1 Tax=Blastopirellula marina
           DSM 3645 RepID=A3ZMY4_9PLAN
          Length = 219

 Score =  113 bits (283), Expect = 5e-24,   Method: Compositional matrix adjust.
 Identities = 74/224 (33%), Positives = 109/224 (48%), Gaps = 7/224 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M + V V SGG DS T L   +    +V  ++ +YGQRH  E+D AR+L  K+G     V
Sbjct: 1   MSKVVAVVSGGMDSATLLYHLIDAGHDVRAISINYGQRHVKELDYARQLCDKVGV-PQIV 59

Query: 61  LDVTLLNEL-AVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
            D++ +N L   +SL+   I +P+     + +  T VP RN++ L+++  +A   +  AV
Sbjct: 60  ADLSAINPLFGANSLSGREIEIPEGHYAEENMKLTVVPNRNMILLSVSIAWAISQECVAV 119

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G    D + YPDCR EF  A++ A  L     +    P + IDK +   +A    +L 
Sbjct: 120 AYGAHSGDHAIYPDCRPEFAAAMDQAAKLCDWSAVELWRPFVDIDKGD---IAQRGSELG 176

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGL-NHYLADKPTVMA 222
           +    T TCY G   + CG C AC  R      H L D    MA
Sbjct: 177 VPYELTWTCYKGL-AEHCGKCGACVERKEAFAKHGLVDPTAYMA 219


>UniRef50_B8HQ99 7-cyano-7-deazaguanine synthase n=1 Tax=Cyanothece sp. PCC 7425
           RepID=QUEC_CYAP4
          Length = 229

 Score =  111 bits (277), Expect = 2e-23,   Method: Compositional matrix adjust.
 Identities = 71/215 (33%), Positives = 112/215 (52%), Gaps = 14/215 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL- 61
           + V++ SGG DSTT L  A     + + ++F+YGQRH+ E+  A  +A   G   H+V+ 
Sbjct: 2   KIVILLSGGLDSTTVLYLARSLGHDCYALSFNYGQRHKQELAAAIAVAQLAGVIQHQVIS 61

Query: 62  -DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
            D+ L    A++  T D      ++  A+ IP T+VP RN +FL+ A  YA  + A  V 
Sbjct: 62  FDLGLWGGSALTDYTLDLPQNRAFKEMAEQIPITYVPARNTIFLSFALSYAEAIGATQVY 121

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADYY 175
            G+   D+SGYPDCR +++ A+     LG       + I+  TPL+ + K     L +  
Sbjct: 122 AGMNAIDYSGYPDCRPDYIAAMQEVYRLGTKQGREGEPIQIITPLIDLTKPAIIQLGN-- 179

Query: 176 GKLDLVRNETLTCYNGFKGD----GCGHCAACNLR 206
            +L +   +T +CY+   G+     CG C +C LR
Sbjct: 180 -RLGVPWAKTWSCYSDGGGNDPPVACGVCDSCRLR 213


>UniRef50_A5ULR3 7-cyano-7-deazaguanine synthase n=5 Tax=Methanobacteriaceae
           RepID=QUEC_METS3
          Length = 227

 Score =  110 bits (276), Expect = 3e-23,   Method: Compositional matrix adjust.
 Identities = 71/223 (31%), Positives = 121/223 (54%), Gaps = 16/223 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           K+A+ V SGG DS        + Y E+H +TF+YGQ+  + E+  + E+  K+G   H+V
Sbjct: 4   KKAITVLSGGLDSCVATCVYAEDY-EIHGITFNYGQKSFKQELKASEEICKKMGF-THEV 61

Query: 61  LDVTLLNELAVSSLTRDS-IPVPDYEPE------ADGIPNTFVPGRNILFLTLAAIYAYQ 113
           +D+  LN+++ SSLT D+ IP   +E         D   + +VPGRNI+F ++A  YA  
Sbjct: 62  IDLPWLNKISNSSLTSDNEIPTLTHEELDDLDICEDTASSVWVPGRNIVFTSIATSYAES 121

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           + AE +I G  + + + +PD   EF+++ N+ +++G  ++I  + P + ++K E   L D
Sbjct: 122 IGAEIIIVGWDKEEAATFPDNSKEFLESFNNMLNVGSPQNIEIKAPAIDLNKDEIVKLGD 181

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
              ++      + +CY G +   CG C +C  R       LAD
Sbjct: 182 ---EIKAPMELSYSCYTGHEKH-CGVCESCMRRKRAFK--LAD 218


>UniRef50_B3G2Q8 Putative uncharacterized protein n=1 Tax=Pseudomonas aeruginosa
           RepID=B3G2Q8_PSEAE
          Length = 222

 Score =  110 bits (275), Expect = 4e-23,   Method: Compositional matrix adjust.
 Identities = 69/208 (33%), Positives = 100/208 (48%), Gaps = 14/208 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           ++ VV+ SGG DST CL+ A  +  EV  +   Y Q H  E+D A     + G    K++
Sbjct: 16  RKIVVLHSGGLDSTVCLLLAHSRGHEVVSLGVSYNQHHHVELDYAAAQCARYGIE-RKIV 74

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            V       V    R    +        G+ + F+PGRN +FL LA+  A  + A+ V T
Sbjct: 75  SVAWSKPARVIPKNRSMAEI------KKGVSSAFLPGRNGIFLMLASAEAAGIGADEVWT 128

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+   DFSGYPDCR EF+++    +S  + K  +   PL    K +   LA    +L L 
Sbjct: 129 GINSVDFSGYPDCRPEFIESFRKMLSYAIPKGPKLSAPLQKKSKPQIAKLAH---RLGLQ 185

Query: 182 RNETLTCYNGFKGDG----CGHCAACNL 205
           R++T +CY     +     CG C AC L
Sbjct: 186 RHDTWSCYRPKIAEAGFVPCGACDACKL 213


>UniRef50_A6DLD5 ExsB protein n=1 Tax=Lentisphaera araneosa HTCC2155
           RepID=A6DLD5_9BACT
          Length = 226

 Score =  110 bits (275), Expect = 4e-23,   Method: Compositional matrix adjust.
 Identities = 75/227 (33%), Positives = 113/227 (49%), Gaps = 14/227 (6%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLG-ARA 57
           MK+A V+ SGG DSTT L   ++   YDEV+ ++FDYGQ+H  E+ VA+     L   + 
Sbjct: 1   MKKAAVLISGGLDSTTLLHHVIKSLGYDEVYALSFDYGQKHSRELIVAKAQCDSLPEVKE 60

Query: 58  HKVLDVTLLNEL--AVSSLTRDSIPVPDYEPEADG---IPNTFVPGRNILFLTLAAIYAY 112
           H VLD++ + +   + S+L +  + VPD +  A+G    P T+VP RN++ L++AA +A 
Sbjct: 61  HNVLDISYMGDFLGSSSALVKGGVEVPDLKDIAEGDLDQPVTYVPNRNMMLLSIAASFAE 120

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 +  G    D  GY DC + F+  +N   SL     +  E P + + K     + 
Sbjct: 121 SRDCRQLYYGAQAQDEYGYWDCTESFLARMNDVFSLNRRTGVHVEAPFVNMSKGHVAKIG 180

Query: 173 DYYGKLDLVRNETLTCYNGFKGDG-CGHCAACNLRANGLNHYLADKP 218
              G   +   +T TCY G  GD  C  C +C  RA        D P
Sbjct: 181 LALG---IDYAQTWTCYRG--GDKPCEVCPSCVERALAFKECGIDDP 222


>UniRef50_B9L187 ExsB protein n=1 Tax=Thermomicrobium roseum DSM 5159
           RepID=B9L187_THERP
          Length = 226

 Score =  109 bits (273), Expect = 6e-23,   Method: Compositional matrix adjust.
 Identities = 79/216 (36%), Positives = 111/216 (51%), Gaps = 16/216 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEV-HCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +RAVV+ SGG DSTT L   L  Y  V + ++F YGQRH  E++ AR +A KL    H+V
Sbjct: 8   QRAVVLLSGGLDSTT-LAYLLDAYGFVLYGLSFRYGQRHERELEAARAIAEKLRFAEHRV 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + + L      S +    IP         GIP+T+VP RN++FL +A  YA  + A+AV 
Sbjct: 67  VTIDLAQWGGSSLVGSGEIP----RQPTSGIPSTYVPVRNLVFLAIAHAYAEAIDADAVA 122

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLG------MAKDIRFETPLMWIDKAETWALADY 174
             V + D+SGYPDCR EF+ A   A  L         + I    P + + K+   A+   
Sbjct: 123 IAVSQVDYSGYPDCRGEFLAAYQQAADLASRRFVETGRRIPVLAPFLDVPKS---AIVRL 179

Query: 175 YGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
             +L +   ET +CY G +   C  C +C LRA   
Sbjct: 180 GLRLGVDYAETWSCYRGGE-RPCQECDSCRLRAQAF 214


>UniRef50_C0CIL2 Putative uncharacterized protein n=1 Tax=Blautia hydrogenotrophica
           DSM 10507 RepID=C0CIL2_9FIRM
          Length = 231

 Score =  107 bits (268), Expect = 3e-22,   Method: Compositional matrix adjust.
 Identities = 74/221 (33%), Positives = 115/221 (52%), Gaps = 16/221 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++A+V+FSGG DSTTCL  A+++Y    V  ++  YGQ+H  E++ AR +    G    +
Sbjct: 3   EKAMVLFSGGVDSTTCLGLAIEKYGVQNVIPLSVQYGQKHEKEVEAARRILEYYGIEGME 62

Query: 60  VLDVTLL---NELAVSSLTRDSIPVPDYEP---EADGIP-NTFVPGRNILFLTLAAIYAY 112
            LD+T +   ++ ++ S +   IP   Y     E +G+P +T+VP RN LFL  AA  A 
Sbjct: 63  -LDLTPIFAYSDCSLLSHSEKEIPEASYAEQLRENEGVPVSTYVPFRNGLFLACAASIAL 121

Query: 113 QVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
             +   +  G    D +G  YPDC + F +++N AV  G  + IR E P + ++KA+  A
Sbjct: 122 SRECSCIYYGAHRDDAAGSAYPDCSESFFESMNRAVYEGSGQQIRIEAPFIKMNKAQIVA 181

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLN 211
                 +L +    T +CY G K   CG C  C  R+    
Sbjct: 182 EGL---RLKVPYEFTWSCYEG-KEKPCGVCGTCIDRSEAFK 218


>UniRef50_A9A531 ExsB family protein n=2 Tax=Thaumarchaeota RepID=A9A531_NITMS
          Length = 224

 Score =  106 bits (265), Expect = 6e-22,   Method: Compositional matrix adjust.
 Identities = 77/217 (35%), Positives = 109/217 (50%), Gaps = 21/217 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AVVVFSGG DS  C V  L+   E++ +TF YGQ+   EI   +  A KLG + HK+
Sbjct: 1   MKKAVVVFSGGVDSV-CAVSFLKSKYELYGITFSYGQKANMEISATKSFAKKLGLKQHKI 59

Query: 61  LDVTLLNELAVSS--LTRDSIPVPD-YEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +D+  + EL   S  LT     +P  +E       +  VP RN +FL++A+ +AY + A 
Sbjct: 60  IDIGFMKELYGDSNVLTSSKRKIPSKFE------YSIVVPIRNAVFLSVASAWAYTLNAS 113

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSL----GMAKDIRFETPLMWIDKAETWALAD 173
            V+ G    D   YPDCR  F K L  A +     G+   IR +  + W    E  + +D
Sbjct: 114 LVVYGAHTGD-KYYPDCRPAFAKKLESAFNQGEIDGINSKIRKKIKI-WSPYREGLSKSD 171

Query: 174 YYGKLDLVRNE----TLTCYNGFKGDGCGHCAACNLR 206
              K   V  +    T +CY+  K   CG C +CN R
Sbjct: 172 LLKKGKKVLGDSIFRTWSCYSN-KKLHCGVCESCNNR 207


>UniRef50_Q3AR02 7-cyano-7-deazaguanine synthase n=12 Tax=Bacteria RepID=QUEC_CHLCH
          Length = 228

 Score =  106 bits (264), Expect = 8e-22,   Method: Compositional matrix adjust.
 Identities = 68/219 (31%), Positives = 105/219 (47%), Gaps = 7/219 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS      A Q   E+  +  +YGQR  + E++  R +      +    +
Sbjct: 2   KAILLLSGGMDSLVTTAIAQQAGFELAAMHVNYGQRTMQRELNSFRAICSHYSIQQRLEI 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +   L ++  SSLT  S+PV     E+  IP ++VP RN  FL++A  +A  + AE +  
Sbjct: 62  NADFLGKIGGSSLTDLSMPVSVANLESHAIPASYVPFRNAGFLSMAVSWAEVIGAERIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE--TPLMWIDKAETWALADYYGKLD 179
           G  E D SGYPDCR  F +A N  + LG   +   E  TPL+ + K   W +     +L 
Sbjct: 122 GAVEEDSSGYPDCRKIFYEAFNRVIELGTKPETHIEVVTPLIALKK---WEIVRKGIELH 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
                + +CY   +G  CG C +C LR         + P
Sbjct: 179 APFAFSWSCYKN-EGQACGVCDSCALRLRAFEQAGMEDP 216


>UniRef50_O29807 7-cyano-7-deazaguanine synthase n=1 Tax=Archaeoglobus fulgidus
           RepID=QUEC_ARCFU
          Length = 239

 Score =  105 bits (262), Expect = 1e-21,   Method: Compositional matrix adjust.
 Identities = 79/217 (36%), Positives = 113/217 (52%), Gaps = 15/217 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV++ SGG DS+T L   L    EVH +TF YGQ+H  EI+ A ++A     R  KV  
Sbjct: 2   KAVMLLSGGIDSSTLLYYLLDGGYEVHALTFFYGQKHSKEIESAEKVAKAAKVRHLKVDI 61

Query: 63  VTLLNELAVSSLT-RDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            T+ + ++  +LT  + +P   Y  E      T VP RN++ L++AA YA ++ A+ V  
Sbjct: 62  STIHDLISYGALTGEEEVPKAFYSEEVQ--RRTIVPNRNMILLSIAAGYAVKIGAKEVHY 119

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLG-MAKDIRFETPLMWIDKAETWALADYYGKLDL 180
               +D+S YPDCR EFVKAL+ AV L  +   +    P + + KA+   L     KL +
Sbjct: 120 AAHLSDYSIYPDCRKEFVKALDTAVYLANIWTPVEVRAPFVDMTKADIVRLG---LKLGV 176

Query: 181 VRNETLTCYNGFKGD-GCGHCAACNLR-----ANGLN 211
               T +CY G  GD  C  C  C  R     ANG+ 
Sbjct: 177 PYELTWSCYEG--GDRPCLSCGTCLERTEAFLANGVK 211


>UniRef50_B8GJL3 7-cyano-7-deazaguanine synthase n=6 Tax=Euryarchaeota
           RepID=QUEC_METPE
          Length = 221

 Score =  105 bits (261), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 66/207 (31%), Positives = 99/207 (47%), Gaps = 7/207 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +AV + SGG DS+T    A      +H +  +YGQR  + E+  A  +A  L A     L
Sbjct: 2   KAVCLLSGGMDSSTLAYVAKDMGYHIHALHLNYGQRTEKKELQSATAIARSLSAEEFIPL 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+        SSLT  +I V  ++     +PNT+VP RN   L++A  +A    A+A+  
Sbjct: 62  DLGYFKAFGKSSLTDPAIDVDVFDENRPELPNTYVPFRNANLLSIATSFAESRDADAIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE--TPLMWIDKAETWALADYYGKLD 179
           GV   D+SGYPDCR  F++A    + LG A     +  TP + + K +          L 
Sbjct: 122 GVQALDYSGYPDCRPAFIQAFQQVMDLGTADGTHIDLLTPFIGLTKTDILRKG---LDLG 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLR 206
           +    T +CY   +   CG C +C+ R
Sbjct: 179 VPYEHTWSCYQN-EEKACGVCGSCHFR 204


>UniRef50_Q1IHK6 7-cyano-7-deazaguanine synthase n=9 Tax=Bacteria RepID=QUEC_ACIBL
          Length = 237

 Score =  104 bits (260), Expect = 2e-21,   Method: Compositional matrix adjust.
 Identities = 79/211 (37%), Positives = 107/211 (50%), Gaps = 13/211 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRA-EIDVARELALKLGARAHK 59
           +AVVV SGG DST C   A+++Y  + +  +   YGQR  A E      +A +LG +   
Sbjct: 10  KAVVVLSGGMDSTVCATLAVREYGAENIGALHVSYGQRTAAREKQAFAAVAERLGIQTRL 69

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDY-EPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            ++      +  S+LT  +I VPD  E     IP T+VP RN   L +A  +A  + A  
Sbjct: 70  AVETPFFRAIGGSALTDANIAVPDAGELIGHEIPVTYVPFRNAHLLAMAVSWAEVLGASK 129

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGM-AKDIRFETPLMWIDKAETWALADYYG- 176
           +  G    D SGYPDCR EF +A N AV  G  A DI   TPL+ + K E  +L    G 
Sbjct: 130 IYIGAVAQDSSGYPDCRPEFYEAYNLAVRRGTKAGDIEVVTPLIALRKHEIVSLGLELGA 189

Query: 177 KLDLVRNETLTCYNGFKGD-GCGHCAACNLR 206
             DL    T +CY+  + D  CG C +C LR
Sbjct: 190 PFDL----TWSCYS--REDCACGVCDSCVLR 214


>UniRef50_D2RGV6 ExsB protein n=2 Tax=Archaeoglobaceae RepID=D2RGV6_ARCPR
          Length = 233

 Score =  104 bits (259), Expect = 3e-21,   Method: Compositional matrix adjust.
 Identities = 66/207 (31%), Positives = 104/207 (50%), Gaps = 8/207 (3%)

Query: 8   FSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARA---HKVLDVT 64
            SGG DS+T L   +++YDE++ +T+ YGQRH  E++ A+ +  K   +A   H++ D++
Sbjct: 1   MSGGIDSSTLLWYLMKRYDEIYVITYIYGQRHAREVEHAKMIVEKAREKAKIHHEIADIS 60

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
            +  L        +  VP+     +    T VP RN++ L +A  YA +V    V     
Sbjct: 61  CIQRLIAKGALTGNEEVPEGFYTDESQKKTIVPNRNMILLAIAVGYAVKVGEREVYYAAH 120

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKD-IRFETPLMWIDKAETWALADYYGKLDLVRN 183
           ++D++ YPDCR EFVKAL+ AV L    D +  + P + + KA+   L     KL +   
Sbjct: 121 KSDYATYPDCRKEFVKALDTAVYLATIFDPVVIKAPFVDMTKADIVKLG---LKLGVPYE 177

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGL 210
            T +CY G +   C  C  C  R    
Sbjct: 178 LTWSCYRG-EERPCLRCGTCLERTEAF 203


>UniRef50_Q2IUE0 7-cyano-7-deazaguanine synthase 2 n=1 Tax=Rhodopseudomonas
           palustris HaA2 RepID=QUEC2_RHOP2
          Length = 210

 Score =  103 bits (257), Expect = 4e-21,   Method: Compositional matrix adjust.
 Identities = 71/208 (34%), Positives = 100/208 (48%), Gaps = 14/208 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K+ +V+ SGG DSTTCL+QA  +  +V  +  DYGQR   E+  A     K     H V+
Sbjct: 4   KKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQRLLVEMMFAEGQCEKYSIPRH-VI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +V          L R        E  A  +   F+PGRNI+FL L   +A  + A+ +  
Sbjct: 63  NVNWQKAERQIPLNRS------VEEMAHSVSPAFLPGRNIVFLGLGHAHAAGIGADELQI 116

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+   DFSGYPDC  +F  +    +++G     +   PL+ + K E   LA   G   L 
Sbjct: 117 GLNCVDFSGYPDCTTQFYDSYCTMLNIGNPGGPKLVAPLLKMSKPEIARLASTLG---LQ 173

Query: 182 RNETLTCYNGFKGDG----CGHCAACNL 205
           RN+T +CY     +G    CG C AC L
Sbjct: 174 RNDTWSCYRPQIREGSIVACGECDACKL 201


>UniRef50_Q8U4N3 7-cyano-7-deazaguanine synthase n=3 Tax=Pyrococcus RepID=QUEC_PYRFU
          Length = 240

 Score =  103 bits (257), Expect = 5e-21,   Method: Compositional matrix adjust.
 Identities = 74/237 (31%), Positives = 116/237 (48%), Gaps = 20/237 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG-QRHRAEIDVARELALKLGARAHKV 60
           +RAVV+FSGG DST CL  A +QYDEV  +T +YG    R    VA   + +L  R  K+
Sbjct: 3   RRAVVLFSGGLDSTACLYWAKKQYDEVIMLTVNYGSNEERVTNKVAEYFSKELDVRL-KI 61

Query: 61  LDVTLLNELAV---SSLTRDSIP------VPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           + +  L E +    SSL    +P      + D E  ++   + ++P RN++ +++AA   
Sbjct: 62  VKLDFLKEFSEIRGSSLVGGEVPRVTAEELEDIEKASETARSVWIPARNLVLISVAASLL 121

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             +    +I G    + + +PD   EFV+ LN A+       ++   PL+ +DK     +
Sbjct: 122 DALGGGDIIVGFNAEEATTFPDNSREFVEKLNEALRFATLNPVKVVAPLIDLDKR---GI 178

Query: 172 ADYYGKLDLVRNETLTCYN--GFKGDG----CGHCAACNLRANGLNHYLADKPTVMA 222
           A    +L+     + +CYN  GF  DG    CG C +C  R  GL   + +  TV A
Sbjct: 179 AKLLKELNAKYEYSNSCYNPKGFTEDGRPIHCGECESCVRRHKGLIEGIGEDKTVYA 235


>UniRef50_UPI0001AF28A8 succinoglycan biosynthesis regulator n=2 Tax=Streptomyces
           filamentosus RepID=UPI0001AF28A8
          Length = 223

 Score =  102 bits (255), Expect = 7e-21,   Method: Compositional matrix adjust.
 Identities = 72/208 (34%), Positives = 104/208 (50%), Gaps = 11/208 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M R + + SGG DS         +  EVH ++ DYGQRHR E D AR  A ++GA  H++
Sbjct: 1   MARVIAIVSGGLDSVVLAHHLHAEGHEVHLLSIDYGQRHRKEHDYARIAADRIGAE-HEI 59

Query: 61  LDVT-LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +D++ + + L   SLT   + VPD    A G  N  VP RN++ L++A   A  VKA+ V
Sbjct: 60  VDLSNVASMLPGYSLTDSQVDVPDQHYTAPGSVNV-VPNRNVILLSVAYGKAAAVKADQV 118

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMA----KDIRFETPLMWIDKAETWALADYY 175
             G    D S  PDC  EF+ + N    +  A      +    P + + K +   L +  
Sbjct: 119 AIGALAGDASTAPDCSAEFISSFNAMEKIACAGYAPAHLEVVAPFIDLHKHDVIRLGE-- 176

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAAC 203
            KL +   ET +C+ G +   CG CAAC
Sbjct: 177 -KLGVDWTETWSCFKGTEVH-CGTCAAC 202


>UniRef50_A6UV33 7-cyano-7-deazaguanine synthase n=12 Tax=Methanococcales
           RepID=QUEC_META3
          Length = 234

 Score =  102 bits (253), Expect = 1e-20,   Method: Compositional matrix adjust.
 Identities = 65/233 (27%), Positives = 115/233 (49%), Gaps = 20/233 (8%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           +AV V SGG DS  C+  A  +  E++ +TFDYGQR  + EI  +++L+   GA  HKV+
Sbjct: 2   KAVCVLSGGLDSAVCMAMAKSEGLELYSITFDYGQRAVKKEIIASKKLSELFGA-VHKVV 60

Query: 62  DVTLLNELAVSSLTRDSIPVP--------DYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           ++  + + + S+LT+    VP        + E   + +   +VP RN++  ++++ +A  
Sbjct: 61  ELPFVKDFSSSALTKKEEEVPTIKNSELDNLEKATETMEKVWVPARNMILFSISSGFAEF 120

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           V A+ + TG+   + + +PD  +EF+   N  +  G    ++ ++PL    K E   +  
Sbjct: 121 VDAKYIYTGLNVEEGATFPDNTEEFLNRFNSVLEYGTLNKVKMKSPLYKYSKKEIVKIGK 180

Query: 174 YYGKLDLVRN-----ETLTCYNGFKGD--GCGHCAACNLRANGLNHYLADKPT 219
              KL+   N      + +CY+  K D   CG C +C  R         + PT
Sbjct: 181 ---KLEEKLNVEFLKYSYSCYHDNKTDFLHCGTCESCMRRKRAFKEAGVEDPT 230


>UniRef50_C1YMB1 PreQ(0) biosynthesis protein QueC n=1 Tax=Nocardiopsis dassonvillei
           subsp. dassonvillei DSM 43111 RepID=C1YMB1_NOCDA
          Length = 243

 Score = 99.8 bits (247), Expect = 6e-20,   Method: Compositional matrix adjust.
 Identities = 80/219 (36%), Positives = 100/219 (45%), Gaps = 10/219 (4%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           VVVFSGG DSTT L         +  VT +YGQRH  E+D AR +A   GA  H +    
Sbjct: 19  VVVFSGGMDSTTLLAHFAALRFRLVAVTVEYGQRHGKEVDAARAIARHYGAEHHILGIAG 78

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
               L  S+LT   + VPD     + + +T VP RN +   LAA  A   +A  V  G+ 
Sbjct: 79  FGPLLRGSALTDARVEVPDGHYAEESMRDTVVPNRNAVLANLAASVAVARRAGTVALGMH 138

Query: 125 ETDFSGYPDCRDEFVKALNHAVSL---GMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
             D   YPDCR  FV AL H V +   G A   R E P M   KA+   +A +  +L   
Sbjct: 139 SGDHFVYPDCRPAFVDALRHLVGVANEGFAAP-RVEAPFMAWSKAD---IARHGVRLGAP 194

Query: 182 RNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPT 219
              + +CY G  GD  CG C  C  R           PT
Sbjct: 195 LEHSWSCYKG--GDIHCGTCGTCYERREAFQEAGLPDPT 231


>UniRef50_B3QPB3 7-cyano-7-deazaguanine synthase n=4 Tax=cellular organisms
           RepID=QUEC_CHLP8
          Length = 227

 Score = 99.8 bits (247), Expect = 7e-20,   Method: Compositional matrix adjust.
 Identities = 66/207 (31%), Positives = 100/207 (48%), Gaps = 7/207 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +AV++ SGG DS      A     E+  +  +YGQR  + E++  R++    G      +
Sbjct: 2   KAVLLVSGGMDSLVATALAHHHGFELAAMHVNYGQRTWQKELECFRQICDHYGIEKRLEI 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D   L  +  SSLT  +IPV   +     IP ++VP RN  FL++A  ++  + A  +  
Sbjct: 62  DAGFLGAIGGSSLTDAAIPVGPADLAGTDIPTSYVPFRNANFLSMAVSWSEVIGANRIYI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE--TPLMWIDKAETWALADYYGKLD 179
           G  E D SGYPDCR  F  A N  +  G   +   E  TPL+ + KAE   +     +LD
Sbjct: 122 GAVEEDSSGYPDCRKVFYDAFNQVIEHGTRPETSIEIVTPLIDMSKAE---IVKRGMELD 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLR 206
              + + +CY   +G  CG C +C  R
Sbjct: 179 APFHFSWSCYKS-EGKACGVCDSCARR 204


>UniRef50_A2STX4 7-cyano-7-deazaguanine synthase n=3 Tax=cellular organisms
           RepID=QUEC_METLZ
          Length = 222

 Score = 98.6 bits (244), Expect = 1e-19,   Method: Compositional matrix adjust.
 Identities = 66/209 (31%), Positives = 104/209 (49%), Gaps = 7/209 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           MK+AV + SGG DSTT    A +   ++  +  +YGQR  R E++ A+++A  LG     
Sbjct: 1   MKKAVCLLSGGMDSTTLAFVAKKMGYDILALHVNYGQRTERKELEAAKKVADVLGVVEFL 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
            + +    +   SSLT  +I V          PNT+VP RN   +++A  Y      EA+
Sbjct: 61  EISLDYFKKFGASSLTDMNIEVEAGVLGRADNPNTYVPFRNGNLISIATSYCESRDGEAI 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMA--KDIRFETPLMWIDKAETWALADYYGK 177
             GV   D +GYPDC   F++A+ HA+ +G    K I   TP + ++K +         +
Sbjct: 121 FIGVQSGDHTGYPDCTPRFIEAMQHAIYVGTQTEKSIELLTPFVMMNKTDILKEG---ME 177

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLR 206
           L +    T +CY+    + CG C++C  R
Sbjct: 178 LCVPYEHTWSCYSE-NDEACGVCSSCLAR 205


>UniRef50_B3T674 Putative ExsB n=1 Tax=uncultured marine crenarchaeote
           HF4000_ANIW141O9 RepID=B3T674_9ARCH
          Length = 233

 Score = 98.2 bits (243), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 70/216 (32%), Positives = 104/216 (48%), Gaps = 19/216 (8%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AVVVFSGG DS +      Q+Y E++ ++F YGQ+   EI  A+  A  LG + HKV
Sbjct: 10  MKKAVVVFSGGIDSVSTCTYLKQKY-ELYGISFLYGQKANQEIKKAKSFAKILGLKEHKV 68

Query: 61  LDVTLLNELA--VSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +D+  + +L    ++LT     +P     +    +  VP RN +FL++A  +A+ + A  
Sbjct: 69  VDINFMKKLYGDTNALTSSKKKIP-----SKFNYSVVVPIRNGIFLSIATAWAFSINASL 123

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHA--------VSLGMAKDIRFETPLMWIDKAETWA 170
           V  G    D   YPDCR  F K +  A        +  G+ K I+  +P       +   
Sbjct: 124 VAYGAHMGD-KNYPDCRPAFSKKIEFAFNQGEIDGIKSGIRKRIQIWSPYKAKLSKKQLL 182

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLR 206
              Y    D + N T +CY   K   CG C +CN R
Sbjct: 183 KKGYDTLGDKIFN-TWSCYKNGKVQ-CGKCESCNNR 216


>UniRef50_B6CWJ6 ToyM n=3 Tax=Bacteria RepID=B6CWJ6_STRRM
          Length = 238

 Score = 98.2 bits (243), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 78/220 (35%), Positives = 105/220 (47%), Gaps = 10/220 (4%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVVV SGG DSTT L         +  VT DYGQRHR EID AR +A   GA  H V+D+
Sbjct: 13  AVVVLSGGMDSTTLLAHYAMLRYRLTAVTVDYGQRHRREIDAARTIAGHYGAE-HHVVDL 71

Query: 64  TLLNE-LAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           + +   L  S+LT   + VP      + +  T VP RN +   +A   A   +A  V  G
Sbjct: 72  SGIGVLLGGSALTDSEVDVPSGHYAEESMRATVVPNRNAILANVAVGAAVARQAGIVALG 131

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSL---GMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +   D   YPDCR  FV+AL+  V +   G A   R E P +   K +   +A Y  +L+
Sbjct: 132 MHAGDHFVYPDCRPAFVEALDGLVRVANEGFATP-RVEAPFIHWSKTD---IARYGSRLE 187

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
              +++ +CY G +   CG C  C  R           PT
Sbjct: 188 APLDKSWSCYRGGELH-CGTCGTCYERREAFRDADVPDPT 226


>UniRef50_D1R557 Putative uncharacterized protein n=1 Tax=Parachlamydia
           acanthamoebae str. Hall's coccus RepID=D1R557_9CHLA
          Length = 221

 Score = 97.8 bits (242), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 72/217 (33%), Positives = 104/217 (47%), Gaps = 13/217 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +A+V+ SGG DS   L +  +Q  E   ++FDYGQRHR E++ A+++A   G   H+V+ 
Sbjct: 2   KAIVLLSGGLDSAVMLARLHEQQIETLAISFDYGQRHRVELEFAKKIADFYGIE-HEVIP 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPE---ADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +  L     S+LT  + P      E      IP T+VP RN LFL  A   A    A+ +
Sbjct: 61  IDPLIFRGGSALTSSATPQKGKSKEDILKQSIPFTYVPARNTLFLAYALAKAEMHGADQI 120

Query: 120 ITGVCETDFSGYPDCRDEFVKA----LNHAVSLGM-AKDIRFETPLMWIDKAETWALADY 174
             G    DF  YPDCR  + +A     N A    +  K  +  TPL+   K E   + + 
Sbjct: 121 HFGSNAMDFLSYPDCRPGYFEAAQELFNRATKQSVEGKAPQIVTPLLKWTKEEI--VKEG 178

Query: 175 YGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGL 210
           Y +L +    T +CY+    G  C  C AC LR +  
Sbjct: 179 Y-RLKVPFELTSSCYDPTANGQPCQQCDACTLRQDAF 214


>UniRef50_C7ML00 ExsB protein n=9 Tax=Bacteria RepID=C7ML00_CRYCD
          Length = 240

 Score = 97.4 bits (241), Expect = 3e-19,   Method: Compositional matrix adjust.
 Identities = 80/225 (35%), Positives = 114/225 (50%), Gaps = 22/225 (9%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDE--VHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
            A+V+ SGG DSTTCL  A+ ++ +  V  V+F YGQRH  E+D A  +A   G   H V
Sbjct: 14  HALVLSSGGVDSTTCLALAVDRFGQANVSTVSFFYGQRHSRELDAAAAVAEHYGVH-HYV 72

Query: 61  LDVTLLNELAVSSLTRDS---IPVPDY--EPEADGIPNTFVPGRNILFLTLAAIYA---Y 112
           LD+  +   + ++L   S   +P   Y  + E DG PNT+VP RN L L+ AA  A   +
Sbjct: 73  LDLASVMHYSNNALMATSTQHVPHGSYAQQMEDDGHPNTYVPFRNGLMLSAAAALAASLF 132

Query: 113 QVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
              + A+  G    D +G  YPDC   F   +  A+S G   ++R ETP +  +KA+  A
Sbjct: 133 PDTSCALYLGAHADDAAGNAYPDCSPAFTAHMGRAISFGTYGNVRLETPFVNQNKAQVVA 192

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAAC-----NLRANGL 210
                 +L +    T +CY G +   CG CA C        ANG+
Sbjct: 193 EGL---RLGVPYEMTWSCYEGGE-KPCGTCATCLDRIAAFEANGV 233


>UniRef50_UPI0001C418E0 queuosine biosynthesis protein QueC n=1 Tax=Methanobrevibacter
           ruminantium M1 RepID=UPI0001C418E0
          Length = 211

 Score = 96.7 bits (239), Expect = 5e-19,   Method: Compositional matrix adjust.
 Identities = 69/210 (32%), Positives = 103/210 (49%), Gaps = 12/210 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K  V++ SGG DSTT L + L + + V  ++F+YGQ+   EI+ A E+  K+    H V 
Sbjct: 3   KDVVLILSGGLDSTTLLYKLLNEGNNVTALSFNYGQKAAIEIERASEIC-KMNDVNHFVF 61

Query: 62  DVTLLNELAVSSLT-RDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           D+  + +L  SSLT +D+  V   EP++     T VP RN + L LA  +A     E V 
Sbjct: 62  DIRNIVDLLSSSLTDKDNDNV--QEPKS-----TVVPSRNTILLELATAFAISNNLEEVY 114

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G  + D   YPD   EF+K +N    +   + I    P +  DK +   LA    +L +
Sbjct: 115 YGAIKADTGDYPDTTPEFLKQINELNKVNNYEYIPVCAPFIDTDKKDVVKLA---LELGV 171

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGL 210
              +T +CY    G  CG C +C  R   +
Sbjct: 172 PIEKTWSCYVNNDGTPCGECFSCKSRIEAI 201


>UniRef50_Q6WI28 ExsB protein n=1 Tax=Vibrio phage KVP40 RepID=Q6WI28_BPKV4
          Length = 238

 Score = 94.0 bits (232), Expect = 4e-18,   Method: Compositional matrix adjust.
 Identities = 63/211 (29%), Positives = 106/211 (50%), Gaps = 11/211 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + V V SGG DST    + + ++  D V  ++F YGQ+   E++ A+    KLG   H++
Sbjct: 2   KVVSVLSGGLDSTILTYKLVDEFGKDNVFALSFYYGQKQSIELEKAKLTCQKLGIE-HRL 60

Query: 61  LDVTLLNELAV---SSLTRDSIPVPDYEPE-ADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
           +D++ L E+     +++    + +P  +    D  P T VP RN+L  T+A  +A     
Sbjct: 61  IDISFLGEVVAPVCANIAGSDVAMPTIQDVLGDPQPPTEVPYRNMLLNTIAFSFAQANGC 120

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            AV TG+   D  GY DC  +F+  +N   +L    +I    P   + KA+  A+     
Sbjct: 121 SAVFTGIQVHDAYGYWDCTQDFLDGMNKISALNRQHEITLHAPFAQMSKADEIAIGV--- 177

Query: 177 KLDLVRNETLTCYNGFK-GDGCGHCAACNLR 206
           +L +   ++LTCY+  + G+ CG C +C  R
Sbjct: 178 ELGVPFEDSLTCYDPNEAGESCGKCPSCAER 208


>UniRef50_Q5JHU5 7-cyano-7-deazaguanine synthase n=6 Tax=Thermococcus
           RepID=QUEC_PYRKO
          Length = 239

 Score = 93.6 bits (231), Expect = 4e-18,   Method: Compositional matrix adjust.
 Identities = 68/237 (28%), Positives = 108/237 (45%), Gaps = 18/237 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MKRAVV+FSGG DST CL  A + YDEV  +T +YG       +   E   K      K+
Sbjct: 1   MKRAVVLFSGGLDSTACLYWAKKNYDEVIMLTVNYGSNEEKVTNKVAEFFSKELDVPLKI 60

Query: 61  LDVTLLNELAV---SSLTRDSIP------VPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           + +  L E +    ++L     P      + + E   +   + +VP RN++ + +AA   
Sbjct: 61  VRLDFLEEFSKLRGTTLVGGETPKVTGEELENIEVAQETAKSVWVPARNVVLIAVAASLL 120

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             +    ++ G    + + +PD   EFV+ +N  +  G   ++R   PL+ +DK     +
Sbjct: 121 DALGGGDIVVGFNAEEGATFPDNTPEFVEKMNEMLKYGTMAEVRVVAPLINLDKR---GI 177

Query: 172 ADYYGKLDLVRNETLTCY--NGFKGDG----CGHCAACNLRANGLNHYLADKPTVMA 222
           A    +L      + +CY   GF  DG    CG C +C  R  GL   + +  TV A
Sbjct: 178 AKLLKELGAKYEYSNSCYMPKGFTEDGKPIHCGECESCVRRHRGLIEGIGEDKTVYA 234


>UniRef50_C4Z7K4 Queuosine biosynthesis protein QueC n=5 Tax=Bacteria
           RepID=C4Z7K4_EUBE2
          Length = 227

 Score = 92.8 bits (229), Expect = 8e-18,   Method: Compositional matrix adjust.
 Identities = 71/217 (32%), Positives = 106/217 (48%), Gaps = 17/217 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +A+V+ SGG DSTT L  A+ +Y  D V  ++  YGQ+H  EI  A+ ++   G      
Sbjct: 2   KALVLSSGGVDSTTALALAVSRYGKDNVIALSVSYGQKHDKEIQAAKAVSAFYGVE-QLF 60

Query: 61  LDVTLLNELAVSSLTRDS---IPVPDY-----EPEADGIPNTFVPGRNILFLTLAAIYAY 112
           LD++ + + +  SL + S   IP   Y     + E +   +T+VP RN LFL+ AA  A 
Sbjct: 61  LDLSKIFQYSNCSLLQQSTEEIPEESYARQIEKTEGEKPVSTYVPFRNGLFLSSAASIAL 120

Query: 113 QVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
                 ++ G    D +G  YPDC   F  A+N A+  G    ++ E P + + KAE   
Sbjct: 121 SKDCSVILYGAHADDSAGFAYPDCSPMFNDAMNQAIFEGSGHQLKVEAPFVNMTKAEVVK 180

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRA 207
           +     +L+     T +CY G K   CG C  C  RA
Sbjct: 181 MGL---ELNAPYELTWSCYEG-KDVPCGKCGTCIDRA 213


>UniRef50_B2UN88 ExsB protein n=6 Tax=Bacteria RepID=B2UN88_AKKM8
          Length = 223

 Score = 92.4 bits (228), Expect = 1e-17,   Method: Compositional matrix adjust.
 Identities = 66/218 (30%), Positives = 102/218 (46%), Gaps = 6/218 (2%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVLDVT 64
           V+ SGG DS+  L  A + +     ++FDY   H   E+  A   A  LG   H+V+D++
Sbjct: 8   VLLSGGLDSSVALHWAHRNHRVRVALSFDYASNHAERELKCAAWQAASLGIE-HRVIDIS 66

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
            ++    S+L   +  +PD   + + +  T VP RN +FL++AA  A    A+ ++    
Sbjct: 67  SISSHLKSALLSGADDIPDGSYDEELMKQTVVPFRNGIFLSIAAGVAESCGAQQLVIAAH 126

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNE 184
             D S YPDCR+EF+ A+  A+ LG    +    P + + K    A+    G +D  R  
Sbjct: 127 SGDHSIYPDCREEFMAAMTEAIRLGTYAGLGILRPFIHMSKGGIAAMGQDLG-VDFSR-- 183

Query: 185 TLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           T +CY G     CG C+ C  R           PT  A
Sbjct: 184 TYSCYKG-GAVHCGVCSTCMERREAFREAGIPDPTAYA 220


>UniRef50_Q8UAM7 7-cyano-7-deazaguanine synthase n=71 Tax=Alphaproteobacteria
           RepID=QUEC_AGRT5
          Length = 236

 Score = 91.7 bits (226), Expect = 2e-17,   Method: Compositional matrix adjust.
 Identities = 70/219 (31%), Positives = 103/219 (47%), Gaps = 7/219 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + +V+ SGG DS +   +   +++ +  V+FDYGQRH+ E+D A + A +LG   H ++D
Sbjct: 2   KTIVICSGGLDSVSLAHKIAAEHELLALVSFDYGQRHKKELDFAADCAKRLGV-PHHLID 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +  +      S   D I VPD     + + +T VP RN + LT+A   A   +A+AV   
Sbjct: 61  IGTIGAHLTGSALTDDIDVPDGHYAEETMRSTVVPNRNAIMLTIAFGLAAAQQADAVAIA 120

Query: 123 VCETDFSGYPDCRDEFVKALN--HAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           V   D   YPDCR  F+ + N   A +L    D+    P + + KA     A  YG    
Sbjct: 121 VHGGDHFIYPDCRPGFIGSFNAMQAHALEGYADVTLFAPYVTVSKAAIVTDAVKYGT--- 177

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
              ET +CY G     CG C  C  R    +      PT
Sbjct: 178 PFGETWSCYKGGLRH-CGRCGTCVERREAFHLAGVTDPT 215


>UniRef50_B9M1I5 ExsB family protein n=4 Tax=Proteobacteria RepID=B9M1I5_GEOSF
          Length = 196

 Score = 89.7 bits (221), Expect = 6e-17,   Method: Compositional matrix adjust.
 Identities = 70/203 (34%), Positives = 100/203 (49%), Gaps = 20/203 (9%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEV--HCVTFDYGQRHR-AEIDVARELALKLGARAHKVL 61
           V + SGG DST  L+  L Q + +  H +  DYGQR R  E+   R    +LG    +V 
Sbjct: 4   VTLVSGGLDST--LIAKLAQEEGLIQHPLFVDYGQRARDQELAACRRGMSELGLPEPRVA 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D++    L  S LT  S+         D + + F PGRN+LFL +AA YA QVKA+A+  
Sbjct: 62  DLSGFGALIHSGLTDSSL---------DIVKDAFTPGRNMLFLLIAAAYAVQVKADAISI 112

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+     S +PD    F++     +   M +DI+   PL    K +  ALA    KL  +
Sbjct: 113 GLLHESTSLFPDQTSAFLRDAEVLIRRCMNRDIKLLAPLASFLKTDVVALA----KLKRI 168

Query: 182 RNETLTCYNGFKGDGCGHCAACN 204
              T +C+ G + + CG C AC 
Sbjct: 169 -GATYSCHTGNE-ETCGTCIACR 189


>UniRef50_Q857D1 Gp3 n=2 Tax=unclassified Siphoviridae RepID=Q857D1_9CAUD
          Length = 235

 Score = 88.6 bits (218), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 74/223 (33%), Positives = 103/223 (46%), Gaps = 10/223 (4%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAH 58
            +  +V+ SGG DS+T L  AL +   D+   +  DYGQRH  E   ++ +A   GA A 
Sbjct: 4   FRSPLVLLSGGMDSSTLLAWALDRPDVDQASALFVDYGQRHVVERASSQLVAQHYGA-AW 62

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
             LD+        S+LT  +  VP      D +  T VP RN   ++ AA  A  +  +A
Sbjct: 63  SELDLRAFGASVNSALTVMAREVPHGHYAEDNMVQTVVPNRNATLISAAAGIAASLGHDA 122

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           V+T V   D + YPDCR EFV A++ A   G    +    P    DKA+   +A   G+L
Sbjct: 123 VLTAVHVGDHAVYPDCRPEFVAAIDMATFAGCG--VHVVAPFQTGDKAD---IARLGGQL 177

Query: 179 DLVRNETLTCYNGFKGDG--CGHCAACNLRANGLNHYLADKPT 219
            +  + T +CY G   D   CG C  C  RA          PT
Sbjct: 178 GVPYHLTWSCYEGGLNDARHCGRCGTCVERAEAFAEAGVPDPT 220


>UniRef50_B1XTB6 ExsB protein n=1 Tax=Polynucleobacter necessarius subsp.
           necessarius STIR1 RepID=B1XTB6_POLNS
          Length = 208

 Score = 88.2 bits (217), Expect = 2e-16,   Method: Compositional matrix adjust.
 Identities = 53/173 (30%), Positives = 82/173 (47%), Gaps = 10/173 (5%)

Query: 13  DSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTLLNELAVS 72
           DSTT L  A       + ++  YGQRH +E+  A+ +A ++G   H+V+++ L      S
Sbjct: 23  DSTTVLALAKDSDYTPYALSVGYGQRHSSELAAAKHIAKQIGVARHEVVNLDL-TRFGGS 81

Query: 73  SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVCETDFSGYP 132
           +LT   I VP    +   IP T+VP RN + L+LA  +   +    +  G    D+SGYP
Sbjct: 82  ALTDTKIAVPTSPGKDHEIPVTYVPARNTILLSLALGWTESLGGLDIFYGANSVDYSGYP 141

Query: 133 DCRDEFVKALNHAVSLGMAKDI---------RFETPLMWIDKAETWALADYYG 176
           DCR E V +  H  +L     +         R   P++ + KA+   L    G
Sbjct: 142 DCRPECVASFEHMANLATKAGVEAINNKNRFRVHAPIVSLTKAQIIQLGSTMG 194


>UniRef50_Q12WI3 7-cyano-7-deazaguanine synthase n=6 Tax=cellular organisms
           RepID=QUEC_METBU
          Length = 233

 Score = 87.4 bits (215), Expect = 4e-16,   Method: Compositional matrix adjust.
 Identities = 61/215 (28%), Positives = 104/215 (48%), Gaps = 16/215 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           R++++ S G DS   L  AL   D    +TFDYGQR  + EI+ +R +    G   H+++
Sbjct: 2   RSILLLSSGLDSVAALAIALDNSDVFMALTFDYGQRSVKKEIEYSRMVCEHYGIE-HRII 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDYE-------PE---ADGIPNTFVPGRNILFLTLAAIYA 111
            ++ L+ +  +SL    + VP+         P+    D   + +VP RN +F+ +AA +A
Sbjct: 61  TLSWLSSITNTSLVNKDLDVPELSINDLIDGPDNITEDSAKSVWVPNRNGVFINIAASFA 120

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
                E VI G    +   +PD   EFV A++   S   A  ++   PL+ +DK    ++
Sbjct: 121 ESHGCEYVIAGFNGEEAKTFPDNSKEFVNAVDECFSYSTANGVKVLAPLIDMDKV---SI 177

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLR 206
                +L      + +CY+G + + CG C +C  R
Sbjct: 178 VRKAMELGAPLEYSWSCYHGAE-EPCGVCESCMRR 211


>UniRef50_D2RLP8 ExsB protein n=3 Tax=Bacteria RepID=D2RLP8_ACIFE
          Length = 232

 Score = 86.3 bits (212), Expect = 8e-16,   Method: Compositional matrix adjust.
 Identities = 72/225 (32%), Positives = 110/225 (48%), Gaps = 23/225 (10%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+A+V+ SGG DSTTC+  A++++  + V  V+  YGQ+HR E++ AR++A   G   H 
Sbjct: 3   KKALVLSSGGVDSTTCVSVAVEEFGKENVSTVSIFYGQKHRKELECARKVAEYYG-LPHY 61

Query: 60  VLDVTLLNELAVSSL---TRDSIPVPDYEPE----ADGIPNTFVPGRNILFLTLAAIYA- 111
            LD++ + + +  SL   + + I    YE +     +G  +T+VP RN L L+  A  A 
Sbjct: 62  ELDLSKIFQYSNCSLLDHSTEEIVHESYEQQIAENGEGKVSTYVPFRNGLMLSAVASLAM 121

Query: 112 --YQVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
             Y+    ++  G    D +G  Y DC + F+ A+  A+SLG         P     KA 
Sbjct: 122 SIYEKDDVSIFIGAHADDAAGNAYADCSEAFIGAMGKAISLGTYNQCHIVAPFASCSKAG 181

Query: 168 TWALA-DYYGKLDLVRNETLTCYNGFKGDG-CGHCAACNLRANGL 210
                 + +   DL    T +CY G  GD  CG C  C  RA   
Sbjct: 182 VVKRGLELHTPYDL----TWSCYEG--GDKPCGTCGTCIDRARAF 220


>UniRef50_C8PJP9 Queuosine biosynthesis protein QueC n=1 Tax=Campylobacter gracilis
           RM3268 RepID=C8PJP9_9PROT
          Length = 391

 Score = 84.3 bits (207), Expect = 3e-15,   Method: Compositional matrix adjust.
 Identities = 52/142 (36%), Positives = 76/142 (53%), Gaps = 8/142 (5%)

Query: 72  SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVCETDFSGY 131
           S    D I     E     +P+T+VP RN +FL++AA  A + + +A+  GV + D SGY
Sbjct: 240 SQSQSDKISPSSGESAFSDLPSTYVPFRNGVFLSIAAALAEKERCDAIFIGVVQEDSSGY 299

Query: 132 PDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETWALADYYG-KLDLVRNETLTC 188
           PDC   F+ A   A+ LG ++D   R +TPL+ + KA+  +LA   G  L+L    T +C
Sbjct: 300 PDCGASFIAAFERALELGTSRDFHPRIKTPLVHLSKAQIVSLALEIGVPLEL----TWSC 355

Query: 189 YNGFKGDGCGHCAACNLRANGL 210
           Y   +   CG C +C LR  G 
Sbjct: 356 YE-REDAACGLCDSCLLRLKGF 376



 Score = 45.1 bits (105), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 30/93 (32%), Positives = 46/93 (49%), Gaps = 1/93 (1%)

Query: 3  RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKVL 61
          RA+ V SGG DS TC   A ++  E+  + FDY QR    E +   ++   LG     +L
Sbjct: 2  RALCVISGGMDSATCAYIARREGYEIVALHFDYDQRTMGKERECFGKICDDLGVAKRLIL 61

Query: 62 DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT 94
          D   + ++  S+LT  ++P+     E  G  NT
Sbjct: 62 DARFIAQIGGSALTDGTLPIRKGAAELCGAANT 94


>UniRef50_D1PYH8 Transcription regulator ExsB n=5 Tax=Prevotella RepID=D1PYH8_9BACT
          Length = 242

 Score = 83.2 bits (204), Expect = 6e-15,   Method: Compositional matrix adjust.
 Identities = 63/210 (30%), Positives = 98/210 (46%), Gaps = 8/210 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKV 60
           + A+++ SGG DS T L +   +      ++FDYG  H A EI  A     +LG   H +
Sbjct: 26  RDAIIIVSGGMDSITMLYEFQDRI--ALGLSFDYGSNHNAREIPYAEMHCKRLGI-PHII 82

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  ++    SSL   +  +P+ +    G+ +T VP RN + L++AA  A     + ++
Sbjct: 83  IPLDFMSRYFKSSLLEGADAIPEGDYNEVGMKSTVVPFRNGIMLSIAAGMAESQNLKYIM 142

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D   YPDCR EF++A++ A   G    I+   P   I K +   +A     L L
Sbjct: 143 MANHSGDHIIYPDCRPEFIRAMSKATQTGTFPGIKILAPYTDITKGD---IACRGKALGL 199

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGL 210
              ET +CY G +   CG C  C  R   L
Sbjct: 200 DYTETWSCYKGGERH-CGKCGTCLERIAAL 228


>UniRef50_B2RJS2 7-cyano-7-deazaguanine synthase n=5 Tax=Bacteria RepID=QUEC_PORG3
          Length = 220

 Score = 81.3 bits (199), Expect = 2e-14,   Method: Compositional matrix adjust.
 Identities = 63/221 (28%), Positives = 99/221 (44%), Gaps = 12/221 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKV 60
           K +++V SGG DS T + +  ++      ++FDYG +H A E+  AR  A +LG   H +
Sbjct: 5   KDSLIVVSGGMDSVTLMYE--KRASIALALSFDYGSKHNARELSFARLHAERLGVE-HLI 61

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  + +   S L      +P+   + + + +T VP RN + L +AA  A       + 
Sbjct: 62  IPLDFIGQYFQSDLLLSGGDIPEGRYDEENMKSTVVPFRNGIMLAVAAGLAESRGLRRIY 121

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D + YPDCR  F++ +  AV  G    +  E P   I K +   +A     L +
Sbjct: 122 IANHFGDHAIYPDCRASFIRPMTEAVRCGTTNGVLIEAPYTDITKTD---IARIGASLGI 178

Query: 181 VRNETLTCYNG--FKGDGCGHCAACNLRANGLNHYLADKPT 219
              ET +CY G  F    CG C  C  R   L+      PT
Sbjct: 179 DYAETWSCYKGGVFH---CGVCGTCVERKEALHDAGIPDPT 216


>UniRef50_C0WAE7 Archaeosine biosynthesis protein queC n=3 Tax=Bacteria
           RepID=C0WAE7_9FIRM
          Length = 233

 Score = 78.2 bits (191), Expect = 2e-13,   Method: Compositional matrix adjust.
 Identities = 68/224 (30%), Positives = 108/224 (48%), Gaps = 21/224 (9%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+A+V+ SGG DSTTC+  A+ +   + V  V+  YGQ+HR E+D A+++A       H 
Sbjct: 3   KKALVLSSGGVDSTTCVSVAVHELGRENVATVSIFYGQKHRKELDAAQKVA-DFYHVPHY 61

Query: 60  VLDVTLLNELAVSSL---TRDSIPVPDYEPE----ADGIPNTFVPGRNILFLTLAAIYA- 111
            LD++ + + +  SL   + + I    YE +     +G  +T+VP RN L L+  A  A 
Sbjct: 62  ELDLSKIFQYSNCSLLSHSTEEIIHESYEDQIAQNGEGKVSTYVPFRNGLMLSAVASLAM 121

Query: 112 --YQVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
             ++     +  G    D +G  Y DC + F  A+  A+S+G  +      P + ++KA 
Sbjct: 122 SLFEKDDVDIYLGAHADDAAGNAYADCSEAFTDAMGKAISIGTYEQCHLVAPFVEVNKA- 180

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGL 210
              +     +L      T +CY G  GD  CG C  C  RA   
Sbjct: 181 --GIVKKGLELGTPYELTWSCYEG--GDRPCGTCGTCIDRARAF 220


>UniRef50_A0B6A6 7-cyano-7-deazaguanine synthase n=4 Tax=Archaea RepID=QUEC_METTP
          Length = 224

 Score = 76.3 bits (186), Expect = 7e-13,   Method: Compositional matrix adjust.
 Identities = 68/228 (29%), Positives = 104/228 (45%), Gaps = 17/228 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           M RAV + SGG DST     A +   +V+ +   YGQ+  R EI+    +A  +GA    
Sbjct: 1   MGRAVCLCSGGLDSTVAATIARRSGMDVYLIHVSYGQQAERREIEAIERIADAIGASDLM 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
              + L     +S+LT     +P  E    +++  P T+V  RN + L++AA YA  + A
Sbjct: 61  CSRIDLFRN--ISALTTQGARIPRGEEVSLDSESTPPTWVYCRNTVLLSMAAAYAEYLGA 118

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMA---KDIRFETPLMWIDKAETWAL-A 172
            ++  G    +   YPD R EFV+  N  +   +A   +  +   PL+ + K +   L A
Sbjct: 119 HSIYVGFNAEEAMSYPDNRPEFVEQFNALLEKAVASFSRPPKVVAPLVDMRKKDIVRLGA 178

Query: 173 DYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPT 219
           D    L+L    T +CY    G+  CG C +C  R  G        PT
Sbjct: 179 DIKAPLEL----TWSCY--LNGEIHCGTCESCQHRRRGFVEAGIPDPT 220


>UniRef50_Q0W7U0 Succinoglycan biosynthesis regulator n=1 Tax=uncultured
           methanogenic archaeon RC-I RepID=Q0W7U0_UNCMA
          Length = 222

 Score = 76.3 bits (186), Expect = 9e-13,   Method: Compositional matrix adjust.
 Identities = 59/226 (26%), Positives = 103/226 (45%), Gaps = 19/226 (8%)

Query: 7   VFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKVLDVTL 65
           + S G DS     +   ++     +TFDYGQR  + EI++AR++  +   R H+V+++  
Sbjct: 1   MLSSGLDSVVAFKKEHDEHGVALAITFDYGQRAASKEIEMARQICARYHVR-HEVIELPW 59

Query: 66  LNELAVSSLTRDSIPVPD-YEPEADGIPN--------TFVPGRNILFLTLAAIYAYQVKA 116
           L ++  ++L      VP+  E E D I           +VP RN  F+ +AA +      
Sbjct: 60  LKQITRTALVSRQAEVPEPTEAELDEIKGRALETAHLVWVPNRNGAFINIAAAFGDAYGY 119

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA-DYY 175
             ++ G    + + +PD    +V A+N +++L   K ++   P++  DK E         
Sbjct: 120 NRIVCGFNAEEGATFPDNTPAYVDAVNKSLALSCEKQVQVVAPVIHFDKEEIIREGVRIE 179

Query: 176 GKLDLVRNETLTCYNGFKGDG-CGHCAACNLRANGLNHYLADKPTV 220
             LDL    + +CY  F G+  CG C +C  RA          P++
Sbjct: 180 APLDL----SWSCY--FAGEKPCGKCESCMRRARAFKRAGVPDPSL 219


>UniRef50_A8AAU7 ExsB protein n=1 Tax=Ignicoccus hospitalis KIN4/I
           RepID=A8AAU7_IGNH4
          Length = 260

 Score = 72.4 bits (176), Expect = 1e-11,   Method: Compositional matrix adjust.
 Identities = 74/232 (31%), Positives = 106/232 (45%), Gaps = 36/232 (15%)

Query: 5   VVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           V + SGG DS T    A+ ++  D    +T  YGQRH  E+  A E+A   GA+ H V+D
Sbjct: 4   VGLVSGGMDSATAFADAINKFNPDVKVALTVVYGQRHARELKAAAEVAKFYGAQ-HLVVD 62

Query: 63  VT-LLNELAVSSLTRDSIPVPDYEPEADG-IPNTFVPGRNILFLTLAAIYAYQVKAE--- 117
           ++ L  ++   S       VP+ E    G +P T+VP RN + L  A     ++  E   
Sbjct: 63  LSDLFEKVKEVSALLGGRGVPEAEELVPGLVPPTYVPQRNAVLLASAGAILEKLMIERND 122

Query: 118 ---AVITGVCETDFSG----YPDCRDEFVKALNHAVSLGMAKDIRFE-----------TP 159
               +  G   TD++     YPD R EFV+A+  A++ G +    F+            P
Sbjct: 123 GLGVISVGFHGTDYAPGEPVYPDTRPEFVEAMERALNEGSSVVFNFKRAGREAKITIYAP 182

Query: 160 LMWIDKAETWALADYYG-KLDLVRNETLTCYNGFKGD-GCGHCAAC--NLRA 207
            + + K +    A   G  L+L    T TCY G  GD  CG C AC   LRA
Sbjct: 183 FVRLKKKDVVKRALELGVPLEL----TWTCYRG--GDRPCGTCPACVSRLRA 228


>UniRef50_C3XFP7 Queuosine biosynthesis protein queC n=1 Tax=Helicobacter bilis ATCC
           43879 RepID=C3XFP7_9HELI
          Length = 213

 Score = 70.1 bits (170), Expect = 5e-11,   Method: Compositional matrix adjust.
 Identities = 60/203 (29%), Positives = 98/203 (48%), Gaps = 15/203 (7%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVLDV 63
           +V+FSGG DSTT L  + ++    H + F Y   H + EID A ++  K    ++ +LD+
Sbjct: 3   LVLFSGGVDSTTALFWSKKEC-ATHALIFKYPSLHNKREIDAAVQIC-KDENVSYTLLDM 60

Query: 64  TLLNELAVSSL---TRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +     S+L   + +SIP   Y  E+  +    VP RN +FL++AA  A  +  + ++
Sbjct: 61  SSVFVGFRSALLQNSSESIPQDSYNLES--MAKLVVPFRNGIFLSVAAGLAESLGYKEIV 118

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D   YPDC   F+ ++N ++  G    ++  +P    DK E   L    G +D 
Sbjct: 119 LANHAGDHPLYPDCTQSFIDSMNESIMQGSGGAVKLCSPFCNWDKKEIVKLGLSLG-VDY 177

Query: 181 VRNETLTCYNGFKGDGCGHCAAC 203
            +  T +CY G    G  HC  C
Sbjct: 178 TK--TYSCYRG----GEKHCNTC 194


>UniRef50_A4J5C7 7-cyano-7-deazaguanine synthase n=4 Tax=Clostridia RepID=QUEC_DESRM
          Length = 228

 Score = 67.8 bits (164), Expect = 3e-10,   Method: Compositional matrix adjust.
 Identities = 54/208 (25%), Positives = 93/208 (44%), Gaps = 12/208 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           +VV+ SGG DS   L  A  + +    +T +Y QR   +   A     +  A  H+V+++
Sbjct: 3   SVVLLSGGLDSAVNLAFARTEGNVKLALTINYRQRAAHKEIAAAAQLARYYAIPHRVIEL 62

Query: 64  TLLNELAVSSLTRDSIPVPDYEPE--------ADGIPNTFVPGRNILFLTLAAIYAYQVK 115
             L E+  ++L R    +P+ + E               +VP RN LF+ +AA +A  + 
Sbjct: 63  PWLAEITKTALVRSESQLPEPKTEELDQLELAGKTAAEVWVPNRNGLFVNIAACFAETLH 122

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
            E V+ G    + + +PD   EF  A + A+    A  ++  +    ++K +  AL    
Sbjct: 123 CEIVVAGFNCEEAATFPDNTPEFALAASAAMQYSTANQVKVLSYTGRLNKKDIVALGQ-- 180

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAAC 203
            +L L      +CY G +   CG C +C
Sbjct: 181 -RLQLPWELIWSCYRG-EALMCGKCESC 206


>UniRef50_Q6MKZ5 7-cyano-7-deazaguanine synthase n=1 Tax=Bdellovibrio bacteriovorus
           RepID=QUEC_BDEBA
          Length = 231

 Score = 67.0 bits (162), Expect = 5e-10,   Method: Compositional matrix adjust.
 Identities = 59/213 (27%), Positives = 101/213 (47%), Gaps = 17/213 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDE-VHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           K+ VV+ S G DST    +A++ + E V  +TF+YGQR  + E++ +  +A  LG   HK
Sbjct: 5   KKVVVLLSAGLDSTVNAYEAIKHHHEIVLALTFNYGQRAAKKELEASANIAKHLGI-PHK 63

Query: 60  VLDVTLLNELAVSSL--------TRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           V+++    +   SSL        T  ++ + + +   +   + +VP RN +FL +AA YA
Sbjct: 64  VVELPWFKDFNKSSLLVEDQAVPTGSAVEIDNQQKSEETAKSVWVPNRNGIFLNIAAAYA 123

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             + A+AVI G    + + +PD   EF++    ++    +  +        + K +   L
Sbjct: 124 EALGADAVIPGFNAEEAATFPDNSREFLEQATKSLWYSTSNHVTVGCYTAHLKKPDIVRL 183

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDG-CGHCAAC 203
                 L +       CY  F GD  CG C +C
Sbjct: 184 GQ---GLKVPWELIWPCY--FSGDKWCGQCESC 211


>UniRef50_A8V022 Trans-regulatory protein ExsB n=1 Tax=Hydrogenivirga sp. 128-5-R1-1
           RepID=A8V022_9AQUI
          Length = 166

 Score = 66.2 bits (160), Expect = 7e-10,   Method: Compositional matrix adjust.
 Identities = 53/169 (31%), Positives = 81/169 (47%), Gaps = 12/169 (7%)

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++D+  L  +  S+LT + + +P  E   D  P T VP RN+ FL++AA +    + E +
Sbjct: 1   MVDIPHLKGIEGSALTDEKVDIPS-EDYPDQPPITTVPMRNLNFLSIAASFCDVYEIENI 59

Query: 120 ITGVCETDFSGYPDCRDEF---VKALNHAVSLGMAKD---IRFETPLMWIDKAETWALAD 173
             G+   D S YPDCR EF    +A+ +A S+ +AK    I+  TP + + K +   L  
Sbjct: 60  GIGIHSVD-SPYPDCRAEFASSAEAVINASSVMVAKKKNRIKVYTPFLGMTKTDVLKLG- 117

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
              +L +   +T +CY G     CG CA C  R           P V A
Sbjct: 118 --LELGVPYEKTYSCYRG-TIPPCGECATCRQRQEAFESVGVKDPLVKA 163


>UniRef50_C9YE01 Putative uncharacterized protein n=1 Tax=Curvibacter putative
          symbiont of Hydra magnipapillata RepID=C9YE01_9BURK
          Length = 143

 Score = 63.5 bits (153), Expect = 5e-09,   Method: Compositional matrix adjust.
 Identities = 35/80 (43%), Positives = 50/80 (62%), Gaps = 9/80 (11%)

Query: 4  AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID----VARELALKLGARAHK 59
          A+V+FSGGQDSTTCL  AL +Y+ V  + FDY QRH  E++    V RE+  +    A K
Sbjct: 5  ALVLFSGGQDSTTCLAHALARYERVETIAFDYRQRHNVELEARLNVLREIKTRFPQWAAK 64

Query: 60 -----VLDVTLLNELAVSSL 74
               +LD+ +L E++ +SL
Sbjct: 65 LGEDHLLDLAVLGEVSETSL 84


>UniRef50_Q981C9 7-cyano-7-deazaguanine synthase n=13 Tax=Thermoprotei
           RepID=QUEC_SULSO
          Length = 464

 Score = 62.4 bits (150), Expect = 1e-08,   Method: Compositional matrix adjust.
 Identities = 58/212 (27%), Positives = 94/212 (44%), Gaps = 16/212 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL- 61
           R +VV SGG DST    + L++  EV  + F+Y   H+AE +  RE   K+       L 
Sbjct: 243 RVLVVASGGLDSTVAATKLLREGHEVTLIHFNY--HHKAE-EKEREAVRKIAEYLQIPLL 299

Query: 62  --DVTLLNELAVSSLTRDSIPVPDYEPEADG--IPNTFVPGRNILFLTLAAIYAYQVKAE 117
             +  L   +  ++L +    +       +G    + +VP RN++F +++   A     +
Sbjct: 300 EINTDLFKIIGHATLIKGGGEIVKDRKGEEGAEFAHEWVPARNLIFFSVSLAIAEAYGYD 359

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNH--AVSLGMAKDIRFETPLMWIDKAETWALADYY 175
           A+ +G+   +   YPD   EF++ LN     + G  K I    P+  + K E   L    
Sbjct: 360 AIASGINLEEAGAYPDNEMEFIRMLNKLSPYATGPNKRIEILMPVGNLVKHEIVKLGYEI 419

Query: 176 G-KLDLVRNETLTCYNGFKGDGCGHCAACNLR 206
           G  L L    T +CY G +   CG C  C +R
Sbjct: 420 GAPLHL----TWSCYEGGQKH-CGKCGPCYMR 446


>UniRef50_Q8TWG9 7-cyano-7-deazaguanine synthase n=1 Tax=Methanopyrus kandleri
           RepID=QUEC_METKA
          Length = 229

 Score = 58.2 bits (139), Expect = 2e-07,   Method: Compositional matrix adjust.
 Identities = 59/232 (25%), Positives = 93/232 (40%), Gaps = 20/232 (8%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYD-EVHCVTFDYGQRHRA-EIDVARELALKLGARAH 58
           M   VV+ SGG DS+     A+++    V C+  DYGQR+   E   A  +A  +GA   
Sbjct: 1   MATTVVLCSGGLDSSVIAKWAVEELGGRVICLFVDYGQRNAPFERRAAERIAEAVGAEFE 60

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            V    L      + +    +P    E   + +   ++P RN   L +AA     +  E 
Sbjct: 61  TVGTFWLRRLCPDNPMFAGRLP---REAGTEDLSANWLPARNWNLLGVAAALCDHLYLEG 117

Query: 119 ------VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 ++ G+   +   +PD   EF  A+  A+  G+    R  +PL  + K     L 
Sbjct: 118 EDDEFHIVWGINAEEAERFPDNTKEFADAVAEALKRGLPSRPRLHSPLAELYKPGIVRLG 177

Query: 173 DYYG-KLDLVRNETLTCYNGFKGDG----CGHCAACNLRANGLNHYLADKPT 219
              G  ++L    +++CYN    D     CG C AC  R         + PT
Sbjct: 178 SELGAPMEL----SVSCYNPIWEDDTPVHCGECEACYHRKRAFERAGIEDPT 225


>UniRef50_A8URJ3 Putative uncharacterized protein n=1 Tax=Hydrogenivirga sp.
           128-5-R1-1 RepID=A8URJ3_9AQUI
          Length = 203

 Score = 55.5 bits (132), Expect = 1e-06,   Method: Compositional matrix adjust.
 Identities = 54/220 (24%), Positives = 85/220 (38%), Gaps = 23/220 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           MK+  V+FS G +ST+ LV  L++ Y  +           R E+  +  L   L  R   
Sbjct: 1   MKKVGVLFSAGVESTSLLVYYLERGYLTLPLYARCGLSWERTELKWSERLWAFLKYRYKN 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++ +              + P    +P    I    +P RNI  +  A ++AY      V
Sbjct: 61  LMPL-------------KTFPASGLQPSKGSIE---IPLRNITLVMGAVVHAYVKGIRKV 104

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G        +PD   +++  L   +S G  ++I  ETP M ++K E   + ++Y K  
Sbjct: 105 ALG--SLGIYPFPDNNRDYLDRLEALISEGARENISLETPFMGMEKWEV--VREFYRKAP 160

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
                T +C     G  CG C  C  R  G        PT
Sbjct: 161 Y--RLTFSCVKPIIGKHCGKCEKCLERKEGFLKAGVPDPT 198


>UniRef50_Q8ZVX7 7-cyano-7-deazaguanine synthase n=7 Tax=Thermoprotei
           RepID=QUEC_PYRAE
          Length = 269

 Score = 53.9 bits (128), Expect = 4e-06,   Method: Compositional matrix adjust.
 Identities = 65/231 (28%), Positives = 97/231 (41%), Gaps = 34/231 (14%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARA----- 57
           R V V SGG DST      L++  +VH ++F YGQ+   E++ A+ L  +L   A     
Sbjct: 8   RVVAVVSGGPDSTCYTALWLKRGCDVHALSFLYGQKAVVEVERAQLLLRRLDGIAAERGW 67

Query: 58  -----HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
                H+V+D++ L EL   +   D    P    E    P   VP RN++   +A  YAY
Sbjct: 68  GRVVEHRVVDLSSLGELWRGTQLTD----PSVSVEQSYTPTVVVPIRNVVMAAVATAYAY 123

Query: 113 QVKAEA-----VITGVCETDFSG--------YPDCRDEFVKALNHAVSLGMAKDIRFETP 159
            V   A     VI G    D           YPDC  E V+A+  A  +   +  R    
Sbjct: 124 TVGRNAGAKTYVILGSHYDDIKPREDTWEPLYPDCSPECVEAMQTAFRICHFRGERGVE- 182

Query: 160 LMWIDKAE----TWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLR 206
            +W    E    +  L   + ++  +  +T +CY   +   CG C +C  R
Sbjct: 183 -IWTPSREGLRKSQLLKMCHQEVGDLIYDTWSCYKSGEAH-CGSCESCKNR 231


>UniRef50_Q24ZT5 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Desulfitobacterium
           hafniense Y51 RepID=QUEC1_DESHY
          Length = 220

 Score = 53.9 bits (128), Expect = 4e-06,   Method: Compositional matrix adjust.
 Identities = 47/210 (22%), Positives = 94/210 (44%), Gaps = 12/210 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKV- 60
           + V++FSGG DST  L   L +  E+  +  +YGQ+ +  E++   ++   L  + + + 
Sbjct: 5   KVVLLFSGGIDSTVLLFWLLSRNYEIFPLFINYGQKSYEGELEAINKILKDLNTKNNLLT 64

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           L++  L  +   +L  +  P  +     +   + F P RN++ L++AA Y Y+++   + 
Sbjct: 65  LNMPELQLVGSGALVGE-YP-KNISSHNEWYASEFFPNRNMILLSIAATYGYKLQISKIA 122

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            GV       Y D    F++A+   ++  +A   R+E    +        + + Y +L +
Sbjct: 123 IGVVG---DSYQDTTRTFLEAMEMTLAQSIA---RYELIAPFAGHPRQKVIEEAY-RLQV 175

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGL 210
               T +C N      C  C +C  R   +
Sbjct: 176 PLKSTFSC-NAMGNRHCLLCTSCYEREKAI 204


>UniRef50_O67015 Putative uncharacterized protein n=1 Tax=Aquifex aeolicus
           RepID=O67015_AQUAE
          Length = 110

 Score = 52.8 bits (125), Expect = 1e-05,   Method: Compositional matrix adjust.
 Identities = 33/115 (28%), Positives = 48/115 (41%), Gaps = 10/115 (8%)

Query: 99  RNILFLTLAAIYAYQVKAEAVITGVCETDFSGYP--DCRDEFVKALNHAVSLGMAKDIRF 156
           RN +  T  A+Y           G        YP  D + EF + L+  +S+ + K+   
Sbjct: 2   RNFILCTAGALYGVPKGINTCAIG----SLGMYPFEDNKKEFFEKLSETISMSLGKEFYI 57

Query: 157 ETPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLN 211
           ETP M + K E       +  L+L    +LTC N   G+ CG C  C  R   L+
Sbjct: 58  ETPFMGMHKHEVIKRYGKFIPLEL----SLTCINPVNGEPCGKCIKCKEREEALS 108


>UniRef50_Q13AQ3 ExsB n=6 Tax=Proteobacteria RepID=Q13AQ3_RHOPS
          Length = 206

 Score = 51.2 bits (121), Expect = 3e-05,   Method: Compositional matrix adjust.
 Identities = 54/215 (25%), Positives = 96/215 (44%), Gaps = 18/215 (8%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLD 62
           A+++ SGG DSTT     +++      + FDYGQ     E +  +E+ L +      +L 
Sbjct: 3   ALLLCSGGLDSTTVGYWLVEKNISFQPLFFDYGQHCVEKEWETLQEV-LPISGLVSPLLR 61

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +  ++E+   S +R  I  P+   E     + ++P R ++F ++ A  A     +AV  G
Sbjct: 62  IN-ISEIFQHSPSR-LIAEPNLWTEHVEDDDLYIPYRTLMFFSVGACIAQTRGLDAVYAG 119

Query: 123 VCETDFSGYPDCRDEFVKALNH-AVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              ++ +   DC   F+  L+  + SLG    +RFE P         W+ AD   + + +
Sbjct: 120 FINSNHAKELDCSAAFLNNLDSLSRSLG---PVRFELPF------RDWSKADVVRRANEL 170

Query: 182 R---NETLTCYNGFKGDGCGHCAACNLRANGLNHY 213
           R    +T +C   F    CG C  C  R + +  +
Sbjct: 171 RVPVGKTYSC-QLFSDTPCGACPNCVERISAIESF 204


>UniRef50_A9U3Q1 Predicted protein n=1 Tax=Physcomitrella patens subsp. patens
           RepID=A9U3Q1_PHYPA
          Length = 290

 Score = 50.8 bits (120), Expect = 4e-05,   Method: Compositional matrix adjust.
 Identities = 52/205 (25%), Positives = 79/205 (38%), Gaps = 22/205 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           +VV+ SGG +S   L         +H +  +YGQR               GA   +    
Sbjct: 6   SVVLMSGGIESAVLLYNWRSSSALLHPLYINYGQR---------------GAAMEERASD 50

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP--GRNILFLTLAAIYAYQVKAEAVIT 121
            + N L +     D   V +   E      +FVP   RN++ L LAA YA QV +  V  
Sbjct: 51  KICNNLGLELSRIDMSAVGNKFRELQHGAKSFVPLPHRNLVILGLAASYASQVGSSKVAL 110

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+ + D   Y      F+ +   + +  +   +    PL    KAE   L     +L + 
Sbjct: 111 GLNKDDLGSYSSASQNFMDSF-RSTAASLEPPVTLIAPLSHFTKAEVIRLGK---RLGVP 166

Query: 182 RNETLTCYNGFKGDGCGHCAACNLR 206
             +T +C  G +   CG C  C  R
Sbjct: 167 FADTYSCMVG-RPVHCGRCHQCRSR 190


>UniRef50_B8GP80 ExsB family protein n=1 Tax=Thioalkalivibrio sp. HL-EbGR7
           RepID=B8GP80_THISH
          Length = 213

 Score = 50.4 bits (119), Expect = 5e-05,   Method: Compositional matrix adjust.
 Identities = 58/204 (28%), Positives = 88/204 (43%), Gaps = 28/204 (13%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVLD 62
           ++++ SGG +S T L    +Q   V  +  DYGQR    E D AR         +H +  
Sbjct: 12  SLLLLSGGIESATLLALESRQR-PVTPLVVDYGQRGALRERDAARA-----QCESHGLTP 65

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V L +  A     RD +            P+  +P RN++ L+LA  YA +   + V+  
Sbjct: 66  VEL-DLSATGGTFRDGL---------LKRPHVPLPHRNLVILSLAVSYAARGGIDRVVLA 115

Query: 123 VCETDFSGYPDCRDEFV---KALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +   D + YP     F+   +AL H +  G+       TPL+  DKA    L    G +D
Sbjct: 116 LNRDDLAAYPSASPGFLEHFRALIHDLQPGL----EITTPLIDRDKAGVIRLGVSLG-VD 170

Query: 180 LVRNETLTCYNGFKGDGCGHCAAC 203
             R  T +C  G++   CG C  C
Sbjct: 171 FAR--TWSCLLGYETH-CGACPQC 191


>UniRef50_Q96ZD9 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Sulfolobus tokodaii
           RepID=QUEC1_SULTO
          Length = 202

 Score = 49.3 bits (116), Expect = 1e-04,   Method: Compositional matrix adjust.
 Identities = 53/218 (24%), Positives = 93/218 (42%), Gaps = 24/218 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS++      ++  +  C+  +YGQR  R ++  ++ +  KL  +   V 
Sbjct: 2   KALLLMSGGLDSSSAAYYYTRRGLDFDCLFINYGQRSARMQLRSSKIICEKLNKKLL-VA 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+  + EL +S      I +  +EP         +  RN++ + +A  +A +   E +I 
Sbjct: 61  DIRKIRELFIS-----DIWLKPHEP---------ITHRNLVIIPIAIAFAKEKGYEEIII 106

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              + D   Y   R E +K L +   LG    ++  TP   + K+    L    G   L 
Sbjct: 107 ASVKEDCE-YEQNRIEIIKELKN---LGEILKVKVSTPFAGMPKSFLLKLGVSAG---LD 159

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
            + T +C  G K   CG C+ C  R           PT
Sbjct: 160 PSLTYSCLLGHKYH-CGQCSQCLKRKEAFKSANIQDPT 196


>UniRef50_Q8DNP8 NADPH-dependent 7-cyano-7-deazaguanine reductase n=26
          Tax=Streptococcus pneumoniae RepID=QUEF_STRR6
          Length = 154

 Score = 48.5 bits (114), Expect = 2e-04,   Method: Compositional matrix adjust.
 Identities = 22/49 (44%), Positives = 32/49 (65%), Gaps = 1/49 (2%)

Query: 22 LQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTLLNELA 70
          +Q Y+ V  VTF YGQ H  EI + RE+A + G R H +LD++LL ++ 
Sbjct: 1  MQHYETVEAVTFAYGQHHHLEIQITREIAKEQGIR-HHILDMSLLGQIT 48


>UniRef50_Q0BZ62 Putative uncharacterized protein n=1 Tax=Hyphomonas neptunium ATCC
           15444 RepID=Q0BZ62_HYPNA
          Length = 188

 Score = 48.1 bits (113), Expect = 2e-04,   Method: Compositional matrix adjust.
 Identities = 56/205 (27%), Positives = 86/205 (41%), Gaps = 35/205 (17%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV---L 61
           VV+ SGG DS  C             V  DYG  H A I+   E      A A KV   L
Sbjct: 10  VVLVSGGIDSAACAAMYANNGGSTTGVFVDYG--HPAAIE---EF-----ASAEKVCAAL 59

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            + LL     +S + D I  P             + GRN L L  +A++A  +K   +  
Sbjct: 60  GIPLLK----TSFSTDRITGPGE-----------IVGRNAL-LIWSAVFASGIKKGVIAI 103

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GV     + Y DC   F+  +++ ++      +R   P +   K+E ++   Y  +  + 
Sbjct: 104 GVHAG--TTYYDCSSTFITRMDNMLAESTNGLVRISAPFVNASKSEVFS---YLQRSKVE 158

Query: 182 RNETLTCYNGFKGDGCGHCAACNLR 206
            + T +C +G K + CG CA+C  R
Sbjct: 159 ASLTYSCESGSK-EPCGICASCQDR 182


>UniRef50_Q989H2 Mll6423 protein n=2 Tax=Proteobacteria RepID=Q989H2_RHILO
          Length = 198

 Score = 47.8 bits (112), Expect = 3e-04,   Method: Compositional matrix adjust.
 Identities = 59/204 (28%), Positives = 88/204 (43%), Gaps = 21/204 (10%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLDVT 64
           ++ SGG DS +    A     +V  +T DYGQ+   AEID A  +A  LG  +H+V+ V 
Sbjct: 5   LLLSGGMDSVSI---AWWLRPDV-ALTIDYGQKPAEAEIDAAGHVAQLLGI-SHEVIRVD 59

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
                  SSL   S  +    P+A    + + P RN L +TLA + A +     ++ G  
Sbjct: 60  ------CSSL--GSGDMAGTAPDAAAPVSEWWPFRNQLLVTLAGMAAVRHGVSRLLIGAL 111

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNE 184
            TD   + D R EFV+  +  +++     I  E P + +D  E   L    G        
Sbjct: 112 ATD-GAHADGRPEFVERASQLMAM-QEGGIVVEAPAIELDAVE---LVRRSGIDQGTLAW 166

Query: 185 TLTCYNGFKGDGCGHCAACNLRAN 208
             +C+ G     CG C  C    N
Sbjct: 167 AHSCHTGNY--ACGRCRGCTKHFN 188


>UniRef50_D2L9Z8 ExsB family protein n=1 Tax=Desulfovibrio sp. FW1012B
           RepID=D2L9Z8_9DELT
          Length = 201

 Score = 47.4 bits (111), Expect = 4e-04,   Method: Compositional matrix adjust.
 Identities = 55/206 (26%), Positives = 91/206 (44%), Gaps = 23/206 (11%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           +K + ++ SGG DST+        +      T DYGQ+   AEI  AR++A ++G  +H 
Sbjct: 2   VKNSAILLSGGMDSTSLAFWKKPNF----AFTIDYGQKAAMAEIRAARQVAKEIGI-SHD 56

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT-FVPGRNILFLTLAAIYAYQVKAEA 118
            + +        SSL    + +    P A   P+T + P RN L +TLAA+ A       
Sbjct: 57  TIAID------CSSLGSGDMAMAAPLPNA---PSTDWWPFRNQLLITLAAMKAICRNINL 107

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           +  G+ ++D S Y D   +FV  ++  +S     +I    P + +  AE   L +     
Sbjct: 108 LYIGLVKSD-SSYKDGCSDFVDKISRTLSF-QEGNIEVMAPAIDMTAAE---LVNMSKIP 162

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACN 204
             +   + +C+       CG C  CN
Sbjct: 163 RTLLAWSHSCH--VSDFACGRCRGCN 186


>UniRef50_D2PHM6 ExsB family protein n=3 Tax=Sulfolobus islandicus
           RepID=D2PHM6_SULIS
          Length = 213

 Score = 47.4 bits (111), Expect = 4e-04,   Method: Compositional matrix adjust.
 Identities = 51/219 (23%), Positives = 88/219 (40%), Gaps = 24/219 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           + +++FS G DS++      ++  +  C+  +YGQ   R ++   + +  KL  R     
Sbjct: 16  KFLLLFSSGLDSSSVAYYYAKKSLDFDCLFINYGQSSVRMQLKYGKIICEKLKRR----- 70

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            + ++N   +     ++  +  +EP         +  RN++ + +A  YA Q   E VI 
Sbjct: 71  -LIIINAKNLGEAFVNNDWLRPHEP---------IIHRNLVIVPIAIAYAKQNGYERVIM 120

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
                D    P+ RD     +     LG    +RFETP +   K   + L     K  L 
Sbjct: 121 ATIREDCEYEPNRRD----VMEKLKELGEILGVRFETPFVNFPK---YLLLKIGIKSGLD 173

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
            + T +C  G K   CG C+ C  R           PT+
Sbjct: 174 PSLTYSCLLGHKYH-CGKCSQCLKRIEAFKQAKIRDPTI 211


>UniRef50_A4YH21 ExsB family protein n=1 Tax=Metallosphaera sedula DSM 5348
           RepID=A4YH21_METS5
          Length = 199

 Score = 46.2 bits (108), Expect = 8e-04,   Method: Compositional matrix adjust.
 Identities = 54/206 (26%), Positives = 84/206 (40%), Gaps = 28/206 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKV 60
           +++ + SGG DST  + +  +  YD   C+T DYGQR  + ++  AR    KLG R  +V
Sbjct: 2   KSLFLVSGGLDSTAGMYRFREMDYD---CMTIDYGQRAFKEQVKYARINCEKLGKRLIEV 58

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                 N   V    R+   +  +EP         +  RN + +  A  YA ++   AV 
Sbjct: 59  ------NMKKVGDRFREGKSLIPHEP---------IRHRNAVTIPFALTYASEMGYSAVY 103

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 +   Y   + E + AL     L  A  I    P + + KA    +   +G   L
Sbjct: 104 VFTVSEECQ-YESNKPEIISALR---KLAEALRIGLIFPFLGLSKAHVLRMGIEHGMDPL 159

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLR 206
               T +C  G +   CG C+ C  R
Sbjct: 160 ---NTYSCLLGHRAH-CGRCSQCESR 181


>UniRef50_D2R400 ExsB family protein n=1 Tax=Pirellula staleyi DSM 6068
           RepID=D2R400_9PLAN
          Length = 236

 Score = 45.1 bits (105), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 55/227 (24%), Positives = 87/227 (38%), Gaps = 19/227 (8%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKLGA---RAHKVL 61
           V+ SGG DS+  + + L++   VH +    G      E+   +     +     R  K+L
Sbjct: 12  VLMSGGLDSSILVGKFLREGRTVHPIYIRTGLFWQSGELPAVQAFLAAIATANLRPLKIL 71

Query: 62  DVTLLNELAVS--SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++ L ++L     SLT    P  D   EA      ++PGRN L L  AA+Y  Q+     
Sbjct: 72  ELPL-DDLYTDHWSLTGSGTPADDTPDEA-----VYLPGRNALLLVKAAVYC-QLHGIGQ 124

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +        S + D   EF      A++ G +  +    P   + K E   L      + 
Sbjct: 125 L-ACAPLGSSPFEDASSEFFTEYQSAMNRGSSSPVVLLRPFGELHKEEVMRLG-----VG 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQ 226
           L    T +C +   G  CG C  C  R         +  TV  A ++
Sbjct: 179 LPLELTFSCIHPVDGTHCGTCNKCFERKEAFRITGIEDKTVYTASRR 225


>UniRef50_D0JAY8 Bifunctional argininosuccinate synthase/acetyltransferase activity
           of N-acetylglutamate synthase n=4 Tax=Bacteroidetes
           RepID=D0JAY8_BLASB
          Length = 588

 Score = 44.7 bits (104), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 18/60 (30%), Positives = 38/60 (63%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V+ +SGG D++ CL   +Q+  EVH V  + G  ++ E++   + A+ +G+++HK ++
Sbjct: 193 KIVLAYSGGLDTSYCLKYLIQEKYEVHTVIINTGGFNKDELEEIEDRAISIGSKSHKTIE 252


>UniRef50_C9YE00 Putative uncharacterized protein (Fragment) n=2 Tax=Proteobacteria
           RepID=C9YE00_9BURK
          Length = 82

 Score = 44.7 bits (104), Expect = 0.002,   Method: Compositional matrix adjust.
 Identities = 32/79 (40%), Positives = 39/79 (49%), Gaps = 18/79 (22%)

Query: 162 WIDKAETWALADYYGK-----------LDLVRNETLTCYNGFK------GDGCGHCAACN 204
           WI+KA+TW LA   G+           +DLV   T TCY G +      G GCG C AC 
Sbjct: 5   WINKAQTWELAHELGERSGVPEGGQALVDLVVEHTHTCYLGDRTHRHAWGYGCGTCPACE 64

Query: 205 LRANGLNHYLADKPTVMAA 223
           LRA G   ++ DK    AA
Sbjct: 65  LRAKGYAGFV-DKVNKAAA 82


>UniRef50_B4U7S9 ExsB family protein n=1 Tax=Hydrogenobaculum sp. Y04AAS1
           RepID=B4U7S9_HYDS0
          Length = 196

 Score = 43.9 bits (102), Expect = 0.004,   Method: Compositional matrix adjust.
 Identities = 50/202 (24%), Positives = 79/202 (39%), Gaps = 16/202 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + S G DS + L   + +  E++ +    G +   E     EL        H    
Sbjct: 2   KVVSLVSSGFDSISLLNYYINKNYEIYPIYVKSGFKWEEE-----ELKHLKDIIDHISKS 56

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEA-DGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
             L+  L VS    D     D+   A     +  +P RN+  L  A  YAY V A  +  
Sbjct: 57  YKLIKPLKVSHAPNDFFE--DFNERAFINDEDVEIPFRNLDLLISALKYAYAVDANLIAI 114

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+       + D    F+K +N   S  M  D + +TP + + K + +   D Y   +L 
Sbjct: 115 GI--MGLVAFKDNSYNFIKTINDLFS--MYGDYKVDTPFLGMSKKDVF---DRYFIKELF 167

Query: 182 RNETLTCYNGFKGDGCGHCAAC 203
            ++   C N   G  CG C+ C
Sbjct: 168 -DKAFCCMNPTDGKECGVCSKC 188


>UniRef50_UPI00016C5260 ExsB n=1 Tax=Gemmata obscuriglobus UQM 2246 RepID=UPI00016C5260
          Length = 232

 Score = 42.7 bits (99), Expect = 0.010,   Method: Compositional matrix adjust.
 Identities = 57/228 (25%), Positives = 82/228 (35%), Gaps = 35/228 (15%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYG------QRHRAEIDVARELALKLGARAHK 59
           V+ SGG DS   L +A++    V  +    G      +R   E  +A   A  L  R  +
Sbjct: 21  VLVSGGLDSAILLAEAVRACPVVFPLYVRSGLHWEAVERAYLERFLAAIAAPNL--RPLR 78

Query: 60  VLDVTLLNELAVS-SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           VLD    +      S T + +P  D   EA      F+PGRN+L L    I+ +      
Sbjct: 79  VLDQPTADVYGAHWSTTGEGVPGADSADEA-----VFLPGRNVLLLAKPLIWCHLNAVPF 133

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           V T    T+   +PD    F       VS  +  D+R   P   +             K+
Sbjct: 134 VATAPLGTN--PFPDATPAFYDGFAGIVSSAVRGDVRVLRPYAALH------------KV 179

Query: 179 DLVR-------NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
           D++R         T +C    +G  CG C  C  R           PT
Sbjct: 180 DVMRRGRGRPLEHTFSCIRPVRGLHCGTCNKCAERRQAFRDAHMTDPT 227


>UniRef50_A6L476 Putative sulfate adenylate transferase, subunit 2 n=4
          Tax=Bacteroides RepID=A6L476_BACV8
          Length = 289

 Score = 40.0 bits (92), Expect = 0.060,   Method: Compositional matrix adjust.
 Identities = 25/83 (30%), Positives = 38/83 (45%), Gaps = 7/83 (8%)

Query: 3  RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
          + +V FSGG+DS  CL+QA +QY  D++  V  D G  H        ++  ++G R    
Sbjct: 2  KIIVSFSGGKDSQACLIQAAKQYGADKIEAVFCDTGWEHPDTYQHITDVCKQMGVRI--- 58

Query: 61 LDVTLLNELAVSSLTRDSIPVPD 83
             TL  E     + +     PD
Sbjct: 59 --TTLKPEFNFIEICKKYRRFPD 79


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_B8E544 7-cyano-7-deazaguanine synthase n=198 Tax=Bacter...   310   2e-83
UniRef50_Q1LTK2 7-cyano-7-deazaguanine synthase n=234 Tax=Bacter...   303   3e-81
UniRef50_UPI0001C171C7 ExsB n=2 Tax=Nostocaceae RepID=UPI0001C171C7   292   9e-78
UniRef50_A4XFR8 7-cyano-7-deazaguanine synthase n=2 Tax=cellular...   289   7e-77
UniRef50_A0LJR5 7-cyano-7-deazaguanine synthase n=4 Tax=Bacteria...   285   7e-76
UniRef50_B2KDX5 ExsB protein n=1 Tax=Elusimicrobium minutum Pei1...   284   1e-75
UniRef50_B9MMB0 7-cyano-7-deazaguanine synthase n=8 Tax=Bacteria...   282   7e-75
UniRef50_A2STX4 7-cyano-7-deazaguanine synthase n=3 Tax=cellular...   279   4e-74
UniRef50_Q8K986 7-cyano-7-deazaguanine synthase n=1 Tax=Buchnera...   278   1e-73
UniRef50_C1D6L0 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria...   277   2e-73
UniRef50_B8GJL3 7-cyano-7-deazaguanine synthase n=6 Tax=Euryarch...   277   2e-73
UniRef50_Q8YM45 7-cyano-7-deazaguanine synthase n=14 Tax=cellula...   276   3e-73
UniRef50_A8EWF5 7-cyano-7-deazaguanine synthase n=27 Tax=Epsilon...   275   7e-73
UniRef50_A3ZMY4 Predicted PP-loop superfamily ATPase n=1 Tax=Bla...   274   1e-72
UniRef50_Q1QCP3 7-cyano-7-deazaguanine synthase n=122 Tax=Bacter...   274   2e-72
UniRef50_A8GPK3 7-cyano-7-deazaguanine synthase n=18 Tax=Proteob...   273   4e-72
UniRef50_B0S9S9 7-cyano-7-deazaguanine synthase n=13 Tax=Bacteri...   271   1e-71
UniRef50_B2I4Y4 7-cyano-7-deazaguanine synthase n=16 Tax=Bacteri...   271   2e-71
UniRef50_C8R2H0 ExsB protein n=1 Tax=Desulfurivibrio alkaliphilu...   269   7e-71
UniRef50_B3QPB3 7-cyano-7-deazaguanine synthase n=4 Tax=cellular...   269   8e-71
UniRef50_Q1QVW9 7-cyano-7-deazaguanine synthase n=23 Tax=Proteob...   269   8e-71
UniRef50_B9Z9V1 ExsB protein n=2 Tax=Halobacteriaceae RepID=B9Z9...   268   1e-70
UniRef50_B8HQ99 7-cyano-7-deazaguanine synthase n=1 Tax=Cyanothe...   268   1e-70
UniRef50_Q55468 7-cyano-7-deazaguanine synthase n=43 Tax=Bacteri...   268   1e-70
UniRef50_A7HHA2 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria...   267   1e-70
UniRef50_O29807 7-cyano-7-deazaguanine synthase n=1 Tax=Archaeog...   267   2e-70
UniRef50_B2UN88 ExsB protein n=6 Tax=Bacteria RepID=B2UN88_AKKM8      266   3e-70
UniRef50_A6DLD5 ExsB protein n=1 Tax=Lentisphaera araneosa HTCC2...   264   1e-69
UniRef50_C0QST3 7-cyano-7-deazaguanine synthase n=7 Tax=Aquifica...   263   2e-69
UniRef50_A6VQW6 7-cyano-7-deazaguanine synthase n=22 Tax=Pasteur...   261   1e-68
UniRef50_Q3AR02 7-cyano-7-deazaguanine synthase n=12 Tax=Bacteri...   260   2e-68
UniRef50_A5A3S4 BcepGomrgp35 n=1 Tax=Burkholderia phage BcepGomr...   259   5e-68
UniRef50_Q1IHK6 7-cyano-7-deazaguanine synthase n=9 Tax=Bacteria...   259   6e-68
UniRef50_Q2N612 7-cyano-7-deazaguanine synthase n=56 Tax=Bacteri...   259   7e-68
UniRef50_A6LIS9 7-cyano-7-deazaguanine synthase n=88 Tax=Bacteri...   258   1e-67
UniRef50_B2RJS2 7-cyano-7-deazaguanine synthase n=5 Tax=Bacteria...   257   2e-67
UniRef50_Q7NCE2 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria...   257   3e-67
UniRef50_D0MDN1 ExsB protein n=1 Tax=Rhodothermus marinus DSM 42...   257   3e-67
UniRef50_Q7M8H4 7-cyano-7-deazaguanine synthase n=2 Tax=Campylob...   256   6e-67
UniRef50_Q6ARX9 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria...   255   9e-67
UniRef50_C1YMB1 PreQ(0) biosynthesis protein QueC n=1 Tax=Nocard...   254   1e-66
UniRef50_B6CWJ6 ToyM n=3 Tax=Bacteria RepID=B6CWJ6_STRRM              254   2e-66
UniRef50_C0CIL2 Putative uncharacterized protein n=1 Tax=Blautia...   253   4e-66
UniRef50_Q1CTN3 7-cyano-7-deazaguanine synthase n=12 Tax=Helicob...   249   4e-65
UniRef50_A5ULR3 7-cyano-7-deazaguanine synthase n=5 Tax=Methanob...   249   6e-65
UniRef50_B9L187 ExsB protein n=1 Tax=Thermomicrobium roseum DSM ...   249   6e-65
UniRef50_C4Z7K4 Queuosine biosynthesis protein QueC n=5 Tax=Bact...   248   7e-65
UniRef50_D2RGV6 ExsB protein n=2 Tax=Archaeoglobaceae RepID=D2RG...   248   1e-64
UniRef50_A6UV33 7-cyano-7-deazaguanine synthase n=12 Tax=Methano...   248   1e-64
UniRef50_D1PYH8 Transcription regulator ExsB n=5 Tax=Prevotella ...   248   1e-64
UniRef50_A0B6A6 7-cyano-7-deazaguanine synthase n=4 Tax=Archaea ...   248   1e-64
UniRef50_Q12WI3 7-cyano-7-deazaguanine synthase n=6 Tax=cellular...   247   3e-64
UniRef50_D1R557 Putative uncharacterized protein n=1 Tax=Parachl...   244   2e-63
UniRef50_B4E7W5 7-cyano-7-deazaguanine synthase n=73 Tax=Bacteri...   243   4e-63
UniRef50_B0TFM0 Exsb protein, putative n=1 Tax=Heliobacterium mo...   242   8e-63
UniRef50_UPI0001AF28A8 succinoglycan biosynthesis regulator n=2 ...   240   2e-62
UniRef50_C6XGK1 ExsB protein n=9 Tax=Bacteria RepID=C6XGK1_LIBAP      239   7e-62
UniRef50_UPI0001C418E0 queuosine biosynthesis protein QueC n=1 T...   238   9e-62
UniRef50_Q857D1 Gp3 n=2 Tax=unclassified Siphoviridae RepID=Q857...   237   3e-61
UniRef50_Q0BQ87 7-cyano-7-deazaguanine synthase n=76 Tax=Bacteri...   236   5e-61
UniRef50_Q5JHU5 7-cyano-7-deazaguanine synthase n=6 Tax=Thermoco...   236   6e-61
UniRef50_Q8U4N3 7-cyano-7-deazaguanine synthase n=3 Tax=Pyrococc...   232   6e-60
UniRef50_D2RLP8 ExsB protein n=3 Tax=Bacteria RepID=D2RLP8_ACIFE      231   2e-59
UniRef50_Q8UAM7 7-cyano-7-deazaguanine synthase n=71 Tax=Alphapr...   231   2e-59
UniRef50_C0WAE7 Archaeosine biosynthesis protein queC n=3 Tax=Ba...   231   2e-59
UniRef50_Q6WI28 ExsB protein n=1 Tax=Vibrio phage KVP40 RepID=Q6...   228   9e-59
UniRef50_C7ML00 ExsB protein n=9 Tax=Bacteria RepID=C7ML00_CRYCD      228   1e-58
UniRef50_A9A531 ExsB family protein n=2 Tax=Thaumarchaeota RepID...   226   5e-58
UniRef50_A8AAU7 ExsB protein n=1 Tax=Ignicoccus hospitalis KIN4/...   225   1e-57
UniRef50_Q0W7U0 Succinoglycan biosynthesis regulator n=1 Tax=unc...   225   1e-57
UniRef50_D1R584 Putative uncharacterized protein n=1 Tax=Parachl...   222   1e-56
UniRef50_Q2IUE0 7-cyano-7-deazaguanine synthase 2 n=1 Tax=Rhodop...   221   2e-56
UniRef50_B3G2Q8 Putative uncharacterized protein n=1 Tax=Pseudom...   219   7e-56
UniRef50_UPI00016988F1 exsB protein n=1 Tax=Endoriftia persephon...   218   2e-55
UniRef50_B3T674 Putative ExsB n=1 Tax=uncultured marine crenarch...   217   3e-55
UniRef50_C3XFP7 Queuosine biosynthesis protein queC n=1 Tax=Heli...   215   1e-54
UniRef50_Q6MKZ5 7-cyano-7-deazaguanine synthase n=1 Tax=Bdellovi...   213   3e-54
UniRef50_Q981C9 7-cyano-7-deazaguanine synthase n=13 Tax=Thermop...   212   6e-54
UniRef50_Q8TWG9 7-cyano-7-deazaguanine synthase n=1 Tax=Methanop...   212   8e-54
UniRef50_A4J5C7 7-cyano-7-deazaguanine synthase n=4 Tax=Clostrid...   211   2e-53
UniRef50_B1XTB6 ExsB protein n=1 Tax=Polynucleobacter necessariu...   198   2e-49
UniRef50_Q96ZD9 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Sulfol...   193   5e-48
UniRef50_D2R400 ExsB family protein n=1 Tax=Pirellula staleyi DS...   187   2e-46
UniRef50_D2PHM6 ExsB family protein n=3 Tax=Sulfolobus islandicu...   182   1e-44
UniRef50_B8GP80 ExsB family protein n=1 Tax=Thioalkalivibrio sp....   180   4e-44
UniRef50_A8V022 Trans-regulatory protein ExsB n=1 Tax=Hydrogeniv...   177   3e-43
UniRef50_Q8ZVX7 7-cyano-7-deazaguanine synthase n=7 Tax=Thermopr...   176   5e-43
UniRef50_C8PJP9 Queuosine biosynthesis protein QueC n=1 Tax=Camp...   176   6e-43
UniRef50_Q24ZT5 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Desulf...   175   9e-43
UniRef50_A9U3Q1 Predicted protein n=1 Tax=Physcomitrella patens ...   175   1e-42
UniRef50_A8URJ3 Putative uncharacterized protein n=1 Tax=Hydroge...   175   1e-42
UniRef50_B9M1I5 ExsB family protein n=4 Tax=Proteobacteria RepID...   175   2e-42
UniRef50_Q13AQ3 ExsB n=6 Tax=Proteobacteria RepID=Q13AQ3_RHOPS        173   5e-42
UniRef50_A4YH21 ExsB family protein n=1 Tax=Metallosphaera sedul...   167   3e-40
UniRef50_D2L9Z8 ExsB family protein n=1 Tax=Desulfovibrio sp. FW...   155   1e-36
UniRef50_Q989H2 Mll6423 protein n=2 Tax=Proteobacteria RepID=Q98...   150   3e-35
UniRef50_Q0BZ62 Putative uncharacterized protein n=1 Tax=Hyphomo...   142   1e-32
UniRef50_O67015 Putative uncharacterized protein n=1 Tax=Aquifex...   106   8e-22
UniRef50_C9YE01 Putative uncharacterized protein n=1 Tax=Curviba...    84   3e-15
UniRef50_Q8DNP8 NADPH-dependent 7-cyano-7-deazaguanine reductase...    55   2e-06

Sequences not found previously or not previously below threshold:

UniRef50_B9ZKA9 ExsB family protein n=1 Tax=Thioalkalivibrio sp....   154   2e-36
UniRef50_UPI00016C5260 ExsB n=1 Tax=Gemmata obscuriglobus UQM 22...   146   4e-34
UniRef50_C7RWC1 ExsB family protein n=4 Tax=Proteobacteria RepID...   136   6e-31
UniRef50_B7GTM4 ExsB family protein n=2 Tax=Actinobacteridae Rep...   133   5e-30
UniRef50_B9LWH7 ExsB family protein n=1 Tax=Halorubrum lacusprof...   126   4e-28
UniRef50_Q2RGB7 ExsB n=1 Tax=Moorella thermoacetica ATCC 39073 R...   126   6e-28
UniRef50_B1ZBL1 ExsB family protein n=4 Tax=Proteobacteria RepID...   119   1e-25
UniRef50_C3NEW9 ExsB family protein n=5 Tax=Sulfolobus RepID=C3N...   114   3e-24
UniRef50_Q12C85 ExsB n=1 Tax=Polaromonas sp. JS666 RepID=Q12C85_...   112   9e-24
UniRef50_C6NX81 Archaeosine biosynthesis protein queC n=1 Tax=Ac...   109   8e-23
UniRef50_A1SFM8 ExsB family protein n=1 Tax=Nocardioides sp. JS6...   106   4e-22
UniRef50_B4U7S9 ExsB family protein n=1 Tax=Hydrogenobaculum sp....   104   3e-21
UniRef50_C5PNW4 ExsB superfamily protein n=1 Tax=Sphingobacteriu...   102   9e-21
UniRef50_B8JF98 PP-loop superfamily ATPase-like protein n=1 Tax=...    79   9e-14
UniRef50_A8L9C0 ExsB family protein n=2 Tax=Actinomycetales RepI...    79   1e-13
UniRef50_Q9RZH7 Putative uncharacterized protein n=1 Tax=Deinoco...    76   7e-13
UniRef50_UPI0001B54B61 PP-loop superfamily ATPase-like protein n...    75   2e-12
UniRef50_C9Q119 Putative uncharacterized protein n=1 Tax=Prevote...    73   1e-11
UniRef50_A3CVZ1 ExsB family protein n=1 Tax=Methanoculleus maris...    72   2e-11
UniRef50_UPI0000382ADB COG0603: Predicted PP-loop superfamily AT...    71   2e-11
UniRef50_Q9V1S6 ThiI thiamin biosynthesis protein thiI n=3 Tax=P...    67   4e-10
UniRef50_A9GG06 Putative uncharacterized protein n=1 Tax=Sorangi...    67   4e-10
UniRef50_Q4UTY2 Putative uncharacterized protein n=2 Tax=Gammapr...    67   6e-10
UniRef50_C6ALB1 Putative uncharacterized protein n=2 Tax=Proteob...    66   8e-10
UniRef50_B5CVE8 Putative uncharacterized protein n=1 Tax=Bactero...    66   8e-10
UniRef50_Q9UWQ2 Putative thiamin biosynthesis protein thiI n=1 T...    66   8e-10
UniRef50_C5CE34 Thiamine biosynthesis/tRNA modification protein ...    66   1e-09
UniRef50_Q8R5Z5 tRNA-specific 2-thiouridylase mnmA 2 n=12 Tax=Fu...    65   2e-09
UniRef50_C6MK22 Putative uncharacterized protein n=2 Tax=Deltapr...    65   2e-09
UniRef50_Q6WHP6 Putative uncharacterized protein n=1 Tax=Vibrio ...    64   3e-09
UniRef50_Q3JDB4 Putative uncharacterized protein n=1 Tax=Nitroso...    63   9e-09
UniRef50_B2IBH3 tRNA-specific 2-thiouridylase mnmA n=141 Tax=roo...    63   1e-08
UniRef50_B0V938 Putative uncharacterized protein n=4 Tax=Acineto...    63   1e-08
UniRef50_Q8PWQ6 Conserved protein n=3 Tax=Methanosarcina RepID=Q...    62   1e-08
UniRef50_Q12C76 Putative uncharacterized protein n=1 Tax=Polarom...    62   2e-08
UniRef50_A5INK8 ExsB family protein n=6 Tax=Thermotogaceae RepID...    62   2e-08
UniRef50_A8S2P7 Putative uncharacterized protein n=9 Tax=Firmicu...    61   2e-08
UniRef50_D0XY41 Putative uncharacterized protein n=1 Tax=Cauloba...    61   2e-08
UniRef50_D0LKW7 Queuosine synthesis-like protein n=1 Tax=Haliang...    61   3e-08
UniRef50_C6J374 Thiamine biosynthesis/tRNA modification protein ...    61   3e-08
UniRef50_Q5JCY1 Probable tRNA sulfurtransferase n=14 Tax=Thermoc...    61   3e-08
UniRef50_B1M991 Putative uncharacterized protein n=2 Tax=Proteob...    61   3e-08
UniRef50_A0PSW2 Putative uncharacterized protein n=1 Tax=Mycobac...    61   4e-08
UniRef50_A0YNH9 Putative uncharacterized protein n=2 Tax=Oscilla...    61   4e-08
UniRef50_Q5WAJ2 Putative uncharacterized protein n=4 Tax=Bacillu...    60   5e-08
UniRef50_C6BXJ5 ExsB family protein n=1 Tax=Desulfovibrio salexi...    60   6e-08
UniRef50_A2BKB5 Probable thiamine biosynthesis protein, ThiI n=1...    60   8e-08
UniRef50_B6YWN1 ThiI-related protein n=5 Tax=Euryarchaeota RepID...    59   8e-08
UniRef50_B9L3Q7 Thiamine biosynthesis/tRNA modification protein ...    59   9e-08
UniRef50_Q3SW10 Putative uncharacterized protein n=1 Tax=Nitroba...    59   9e-08
UniRef50_A8AM74 Putative uncharacterized protein n=8 Tax=Gammapr...    59   9e-08
UniRef50_Q0C5H6 Putative uncharacterized protein n=1 Tax=Hyphomo...    59   1e-07
UniRef50_Q9ZJQ0 tRNA-specific 2-thiouridylase mnmA n=22 Tax=Camp...    59   1e-07
UniRef50_Q8TWX3 ATP pyrophosphatase involved in thiamine biosynt...    59   1e-07
UniRef50_B9QRC5 Putative uncharacterized protein n=1 Tax=Labrenz...    59   1e-07
UniRef50_A6LQW7 ExsB family protein n=4 Tax=Clostridium RepID=A6...    59   1e-07
UniRef50_Q13XN6 Putative uncharacterized protein n=1 Tax=Burkhol...    59   1e-07
UniRef50_C9YE00 Putative uncharacterized protein (Fragment) n=2 ...    59   2e-07
UniRef50_O29382 Probable tRNA sulfurtransferase n=2 Tax=Archaeog...    59   2e-07
UniRef50_B5YCZ9 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    58   2e-07
UniRef50_A4T1U6 Putative uncharacterized protein n=2 Tax=Actinom...    58   2e-07
UniRef50_Q72HP1 Probable tRNA sulfurtransferase n=3 Tax=Thermus ...    58   2e-07
UniRef50_Q5P5F5 Putative uncharacterized protein n=1 Tax=Aromato...    58   2e-07
UniRef50_Q65G52 Probable tRNA sulfurtransferase n=20 Tax=Bacilla...    58   3e-07
UniRef50_Q9Y933 GMP synthase [glutamine-hydrolyzing] n=5 Tax=Arc...    58   3e-07
UniRef50_C5A5U2 Predicted ATPase of the PP-loop superfamily n=8 ...    58   3e-07
UniRef50_A3DLP8 Thiamine biosynthesis protein n=1 Tax=Staphyloth...    58   3e-07
UniRef50_A6KXY6 Argininosuccinate synthase n=32 Tax=Bacteroidale...    57   6e-07
UniRef50_Q0A9U9 Thiamine biosynthesis/tRNA modification protein ...    56   7e-07
UniRef50_C5A340 P-loop ATPase, MJ1599 type n=8 Tax=Thermococcace...    56   8e-07
UniRef50_A0B2R8 Putative uncharacterized protein n=4 Tax=Burkhol...    56   1e-06
UniRef50_UPI0001BC5A6B tRNA (5-methylaminomethyl-2-thiouridylate...    56   1e-06
UniRef50_A6Q239 tRNA-specific 2-thiouridylase mnmA n=2 Tax=Epsil...    56   1e-06
UniRef50_B2A8M8 Probable tRNA sulfurtransferase n=2 Tax=Clostrid...    55   2e-06
UniRef50_B9JNY0 Putative uncharacterized protein n=1 Tax=Agrobac...    55   2e-06
UniRef50_Q1LLD1 Putative uncharacterized protein n=1 Tax=Cupriav...    55   2e-06
UniRef50_A3DEB6 Probable tRNA sulfurtransferase n=34 Tax=Bacteri...    54   3e-06
UniRef50_B7H703 Probable tRNA sulfurtransferase n=75 Tax=Bacteri...    54   3e-06
UniRef50_D1UGP9 Putative uncharacterized protein n=1 Tax=Burkhol...    54   3e-06
UniRef50_Q12W34 ATPase of the PP-loop superfamily n=1 Tax=Methan...    54   3e-06
UniRef50_C7X359 Putative uncharacterized protein n=1 Tax=Enteroc...    54   3e-06
UniRef50_B1L455 ExsB family protein n=1 Tax=Candidatus Korarchae...    54   4e-06
UniRef50_D1B2I0 Queuosine synthesis-like protein n=2 Tax=Campylo...    54   5e-06
UniRef50_C9RQ01 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    54   5e-06
UniRef50_B3QQJ9 Putative uncharacterized protein n=1 Tax=Chlorob...    54   5e-06
UniRef50_A9A452 ExsB family protein n=2 Tax=Thaumarchaeota RepID...    53   6e-06
UniRef50_A8AA65 Argininosuccinate synthase n=5 Tax=Archaea RepID...    53   7e-06
UniRef50_Q0AM89 Putative uncharacterized protein n=1 Tax=Maricau...    53   7e-06
UniRef50_Q7URE0 Putative uncharacterized protein n=1 Tax=Rhodopi...    53   8e-06
UniRef50_D0MEN6 Asparagine synthase (Glutamine-hydrolyzing) n=1 ...    53   8e-06
UniRef50_B8D4I6 tRNA sulfurtransferase n=1 Tax=Desulfurococcus k...    53   9e-06
UniRef50_C8WW19 Thiamine biosynthesis/tRNA modification protein ...    53   1e-05
UniRef50_A8EUQ0 Thiamine biosynthesis protein ThiI n=4 Tax=Prote...    53   1e-05
UniRef50_A1U6U9 tRNA sulfurtransferase n=11 Tax=Gammaproteobacte...    53   1e-05
UniRef50_Q5SH30 NH(3)-dependent NAD(+) synthetase n=3 Tax=Thermu...    53   1e-05
UniRef50_B5XVJ1 Putative uncharacterized protein n=1 Tax=Klebsie...    53   1e-05
UniRef50_A1ZJJ3 Asparagine synthase family n=1 Tax=Microscilla m...    53   1e-05
UniRef50_C8WPE1 ExsB family protein n=1 Tax=Eggerthella lenta DS...    52   1e-05
UniRef50_C9YDI6 Putative uncharacterized protein n=1 Tax=Curviba...    52   1e-05
UniRef50_D1CE79 Argininosuccinate synthase n=2 Tax=Bacteria RepI...    52   2e-05
UniRef50_UPI0001C31893 argininosuccinate synthase n=1 Tax=Conexi...    52   2e-05
UniRef50_A5CYW2 ATP-utilizing enzymes of the PP-loop superfamily...    52   2e-05
UniRef50_C7M0G9 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl...    52   2e-05
UniRef50_A7HL33 Thiamine biosynthesis/tRNA modification protein ...    52   2e-05
UniRef50_D2RFP8 ExsB family protein n=1 Tax=Archaeoglobus profun...    52   2e-05
UniRef50_C9LN41 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    52   2e-05
UniRef50_Q8TUR4 Predicted ATPase of the PP-loop superfamily impl...    51   2e-05
UniRef50_B7J5X6 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Acidi...    51   2e-05
UniRef50_C7DBG3 tRNA(Ile)-lysidine synthase n=1 Tax=Thalassiobiu...    51   2e-05
UniRef50_C6CRM5 tRNA(Ile)-lysidine synthetase n=1 Tax=Paenibacil...    51   2e-05
UniRef50_P49915 GMP synthase [glutamine-hydrolyzing] n=87 Tax=Eu...    51   3e-05
UniRef50_Q2LWA6 tRNA-specific 2-thiouridylase mnmA 1 n=1 Tax=Syn...    51   3e-05
UniRef50_Q1AS35 Argininosuccinate synthase n=69 Tax=cellular org...    51   3e-05
UniRef50_A7GTS8 Probable tRNA sulfurtransferase n=5 Tax=Firmicut...    51   3e-05
UniRef50_A8ES35 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Eps...    51   4e-05
UniRef50_B9B2T1 Putative uncharacterized protein n=2 Tax=Burkhol...    51   4e-05
UniRef50_C6HVS1 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    51   4e-05
UniRef50_B6BIH0 tRNA(Ile)-lysidine synthetase n=1 Tax=Campylobac...    51   4e-05
UniRef50_D2RUT9 Argininosuccinate synthase n=1 Tax=Haloterrigena...    51   5e-05
UniRef50_C1XLL9 Asparagine synthase, glutamine-hydrolyzing n=1 T...    51   5e-05
UniRef50_Q64E49 PP-ATPase subunit of GMP synthase n=1 Tax=uncult...    50   5e-05
UniRef50_D0NFB6 tRNA(Ile)-lysidine synthase, putative n=1 Tax=Ph...    50   5e-05
UniRef50_C7DGN0 Thiamine biosynthesis protein n=1 Tax=Candidatus...    50   5e-05
UniRef50_Q2NEC4 Argininosuccinate synthase n=4 Tax=Methanobacter...    50   6e-05
UniRef50_C7N321 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    50   6e-05
UniRef50_D1N6V4 Thiamine biosynthesis/tRNA modification protein ...    50   6e-05
UniRef50_UPI000194D842 PREDICTED: argininosuccinate synthetase 1...    50   6e-05
UniRef50_Q09580 Probable GMP synthase [glutamine-hydrolyzing] n=...    50   7e-05
UniRef50_C5EME3 Asparagine synthase n=4 Tax=Bacteria RepID=C5EME...    50   7e-05
UniRef50_Q5LIS5 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Bac...    50   7e-05
UniRef50_A0LE34 Argininosuccinate synthase n=5 Tax=Bacteria RepI...    50   8e-05
UniRef50_A6D100 Putative uncharacterized protein n=1 Tax=Vibrio ...    50   8e-05
UniRef50_D1J9C4 Putative uncharacterized protein n=1 Tax=uncultu...    50   8e-05
UniRef50_Q8R5X3 tRNA-specific 2-thiouridylase mnmA 1 n=16 Tax=Fu...    50   8e-05
UniRef50_A4X6Q9 Asparagine synthase (Glutamine-hydrolyzing) n=3 ...    50   8e-05
UniRef50_A5GE36 tRNA-specific 2-thiouridylase mnmA n=20 Tax=Bact...    49   9e-05
UniRef50_B1I677 tRNA-specific 2-thiouridylase mnmA n=1 Tax=Candi...    49   9e-05
UniRef50_C0GFP8 Asparagine synthase n=1 Tax=Dethiobacter alkalip...    49   9e-05
UniRef50_C9RAV2 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    49   1e-04
UniRef50_A9BIS3 tRNA-specific 2-thiouridylase mnmA n=1 Tax=Petro...    49   1e-04
UniRef50_P59606 Argininosuccinate synthase n=22 Tax=Proteobacter...    49   1e-04
UniRef50_A5UKF9 ATP-utilizing enzyme, PP-loop superfamily n=3 Ta...    49   1e-04
UniRef50_A8AAD7 PP-loop domain protein n=1 Tax=Ignicoccus hospit...    49   1e-04
UniRef50_C7RPK0 Putative uncharacterized protein n=1 Tax=Candida...    49   1e-04
UniRef50_B5YGH6 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    49   1e-04
UniRef50_UPI0001C41AAB PP-loop family protein n=1 Tax=Methanobre...    49   1e-04
UniRef50_B3T2X9 Putative tRNA methyl transferase n=3 Tax=environ...    49   1e-04
UniRef50_A5FHA9 tRNA-specific 2-thiouridylase mnmA n=26 Tax=Bact...    49   1e-04
UniRef50_B1VAX7 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Bacte...    49   1e-04
UniRef50_Q9YEW8 Probable tRNA sulfurtransferase n=1 Tax=Aeropyru...    49   1e-04

>UniRef50_B8E544 7-cyano-7-deazaguanine synthase n=198 Tax=Bacteria RepID=QUEC_SHEB2
          Length = 240

 Score =  310 bits (796), Expect = 2e-83,   Method: Composition-based stats.
 Identities = 154/222 (69%), Positives = 186/222 (83%), Gaps = 1/222 (0%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AVVVFSGGQDSTTCL+QAL QYDEVH +TFDYGQRHR EI+VA+ LA +L   +HKV+D
Sbjct: 13  KAVVVFSGGQDSTTCLIQALTQYDEVHGITFDYGQRHREEIEVAKSLAKRLKITSHKVMD 72

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           VTLLNELA+S+LTRD+IPV  +E   +G+PNTFVPGRNILFLTLA IYAYQ+ AEA+ITG
Sbjct: 73  VTLLNELAISALTRDAIPV-SHELMENGLPNTFVPGRNILFLTLAGIYAYQLGAEAIITG 131

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           VCETDFSGYPDCR++FVKA+  A+  GM K ++  TPLMW++KA+TWALAD Y +LDLVR
Sbjct: 132 VCETDFSGYPDCRNDFVKAMESALVQGMDKQLKIITPLMWLNKAQTWALADKYQQLDLVR 191

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           + TLTCYNG  GDGCG C AC+LR  GL+ Y+ DK  VMA++
Sbjct: 192 HHTLTCYNGVIGDGCGDCPACHLRKRGLDEYMQDKTAVMASL 233


>UniRef50_Q1LTK2 7-cyano-7-deazaguanine synthase n=234 Tax=Bacteria RepID=QUEC_BAUCH
          Length = 238

 Score =  303 bits (777), Expect = 3e-81,   Method: Composition-based stats.
 Identities = 154/231 (66%), Positives = 187/231 (80%), Gaps = 1/231 (0%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +K+ VVVFSGGQDSTTCL+QAL QYD+V+C+TFDYGQRHRAEI +AR L + LGA  H++
Sbjct: 7   IKKTVVVFSGGQDSTTCLIQALTQYDQVYCITFDYGQRHRAEICIARRLTITLGAYDHQI 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEA-DGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +D + LN LA SSLTR++I VP  E    +   +TFVPGRNI+FLTLAAIYAYQVKAE +
Sbjct: 67  IDTSALNVLAASSLTRENIIVPLAEKNHMNSSLSTFVPGRNIVFLTLAAIYAYQVKAEVI 126

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           ITGVCETDFSGYPDCR++F++ALN AV+LGMA +I F +PLMW++KAETWALADYY KLD
Sbjct: 127 ITGVCETDFSGYPDCREKFIQALNKAVNLGMAYNIHFISPLMWLNKAETWALADYYQKLD 186

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTGL 230
           LV NETLTCYNG +G GCG C +C+LRA GL  Y       M+++K KTGL
Sbjct: 187 LVCNETLTCYNGIQGKGCGFCTSCSLRARGLETYKQQPKLTMSSLKAKTGL 237


>UniRef50_UPI0001C171C7 ExsB n=2 Tax=Nostocaceae RepID=UPI0001C171C7
          Length = 282

 Score =  292 bits (747), Expect = 9e-78,   Method: Composition-based stats.
 Identities = 81/232 (34%), Positives = 119/232 (51%), Gaps = 13/232 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + +AV++ SGG DS+T L QA     E + ++FDY QRHR E+  A  +A  L    H++
Sbjct: 56  IMKAVILLSGGLDSSTVLYQARADGYECYVISFDYHQRHRRELQSAISIAQSLEVVEHQL 115

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +    L     S+LT +SIPVP   P    +  IP T+VP RN +FL+ A  YA  ++AE
Sbjct: 116 VTFD-LRSWGGSALTDNSIPVPQNRPLDQMSGSIPITYVPARNTIFLSFALAYAEAIRAE 174

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALA 172
            V  GV   D+SGYPDCR +++ A+     LG       K I    PL+ + K++   L 
Sbjct: 175 RVYIGVNALDYSGYPDCRPDYIHAMQEVFRLGTKQGREGKRIDIVAPLINLTKSQIIQLG 234

Query: 173 DYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           +    L +    T +CY+G +   CG C +C LR    +    + P     M
Sbjct: 235 NL---LGVPWELTWSCYSGDQ-QPCGVCDSCQLRWAAFHELGLEDPVKQQGM 282


>UniRef50_A4XFR8 7-cyano-7-deazaguanine synthase n=2 Tax=cellular organisms
           RepID=QUEC_CALS8
          Length = 225

 Score =  289 bits (739), Expect = 7e-77,   Method: Composition-based stats.
 Identities = 74/219 (33%), Positives = 112/219 (51%), Gaps = 5/219 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVVV SGG DSTT L     Q  E + ++F YGQ+H  E++ A++    L    HK++D
Sbjct: 2   RAVVVLSGGMDSTTLLYDVKNQGYETYTISFLYGQKHSKELEFAKKTCELLKV-PHKIVD 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++   +LA S+LT+ S  VP+     + +  T VP RN++FL+LA  YA  +KA+ +  G
Sbjct: 61  ISFFADLAPSALTKSSWSVPEGYYTDESMKQTVVPNRNMVFLSLATSYAISLKAQKLFYG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+A+  ++ L   + +  E P + + K +   +     KL +  
Sbjct: 121 AHAGDHPIYPDCRKEFVEAMKRSILLCDYQIVELEAPYVDLKKEDILKIG---LKLGVDY 177

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           + T +CY G +   CG C  C  R           P   
Sbjct: 178 SLTWSCYKGGE-KACGRCGTCTERIEAFRKIGVKDPIEY 215


>UniRef50_A0LJR5 7-cyano-7-deazaguanine synthase n=4 Tax=Bacteria RepID=QUEC_SYNFM
          Length = 234

 Score =  285 bits (730), Expect = 7e-76,   Method: Composition-based stats.
 Identities = 86/230 (37%), Positives = 122/230 (53%), Gaps = 15/230 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           ++AVV+FSGG DSTTCL  A  +  E + ++F Y QRH  E++ AR  A  LGAR H VL
Sbjct: 7   RKAVVLFSGGIDSTTCLAVAKAEGFEAYALSFFYHQRHGVEVEAARRSAPALGAREHLVL 66

Query: 62  DVTLLNELAVSSLTRDSIPVP---DYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           ++  L+ +  S+LT     VP     E  +D IP T+VP RN +FL+ A  +A  + A  
Sbjct: 67  ELP-LDRIGGSALTGHE-EVPKDVPVEKMSDRIPATYVPARNTIFLSFALAWAEVLGAAD 124

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAK------DIRFETPLMWIDKAETWALA 172
           +  GV   D+SGYPDCR E++ A     +L + +       I+  TPL+ + K E     
Sbjct: 125 IFIGVNALDYSGYPDCRPEYIAAFERMANLAIRETVEGRMRIKVHTPLIRLTKGEIIKRG 184

Query: 173 DYYGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGLNHYLADKPTVM 221
               +L L  + T +CY+   +G  CGHC +C LR  G        PT  
Sbjct: 185 ---LELGLDYSLTHSCYDPDARGLACGHCDSCILRKKGFMEAGIPDPTRY 231


>UniRef50_B2KDX5 ExsB protein n=1 Tax=Elusimicrobium minutum Pei191
           RepID=B2KDX5_ELUMP
          Length = 484

 Score =  284 bits (728), Expect = 1e-75,   Method: Composition-based stats.
 Identities = 86/216 (39%), Positives = 121/216 (56%), Gaps = 8/216 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K+AVV+FSGG DST CL  AL++  E + ++  YGQ+H  EI+VA+ LA + G   HK +
Sbjct: 269 KKAVVLFSGGLDSTVCLYWALKEGYECYALSVAYGQKHLKEIEVAQRLAKQAGV-THKHI 327

Query: 62  DVTLLNELAVSSLTRD-SIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++ L      +SL     IP    E     IP+T+VP RN++F++L A +A  + AEAV+
Sbjct: 328 EINLPWLAEATSLVGGAEIPATPMEQIGKSIPSTYVPARNLIFVSLGASWADSIGAEAVV 387

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKL 178
            G    D+SGYPDC  +F K L+ AV +G    K     TP++ ++KAE   L     KL
Sbjct: 388 LGPNAVDYSGYPDCTPQFYKPLSEAVRVGTRLGKKFEILTPIIKLNKAEIVKLGR---KL 444

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYL 214
                +T TCYNG +   CG C +C LR  G     
Sbjct: 445 GAPLEQTWTCYNGGE-KPCGVCESCKLRRKGFEDAG 479


>UniRef50_B9MMB0 7-cyano-7-deazaguanine synthase n=8 Tax=Bacteria RepID=QUEC_ANATD
          Length = 221

 Score =  282 bits (722), Expect = 7e-75,   Method: Composition-based stats.
 Identities = 71/219 (32%), Positives = 107/219 (48%), Gaps = 5/219 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVVV SGG DSTT L     Q  EV  ++F YGQ+H  E++ A++    L    +K++D
Sbjct: 2   RAVVVLSGGMDSTTLLYDVKSQGYEVFAISFLYGQKHSKELEFAKKTCSLLSI-PYKIVD 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++   ELA S+LT     VP+       +  T VP RN++ L+++A YA  + A+ +  G
Sbjct: 61  ISFFAELAPSALTTPDWNVPEGYYTDSTMKQTVVPNRNMVLLSISAAYAISLGAKKLFYG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+A+ +A+ L     +  E P + + K +         +L +  
Sbjct: 121 AHAGDHPIYPDCRKEFVEAMKNALYLADYIGLELEAPYVDMKKEDILRRG---LELGVNY 177

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           + T +CY G +   CG C  C  R           P   
Sbjct: 178 SLTWSCYKGGQ-KACGRCGTCTERIEAFKKVGVKDPIEY 215


>UniRef50_A2STX4 7-cyano-7-deazaguanine synthase n=3 Tax=cellular organisms
           RepID=QUEC_METLZ
          Length = 222

 Score =  279 bits (715), Expect = 4e-74,   Method: Composition-based stats.
 Identities = 67/226 (29%), Positives = 106/226 (46%), Gaps = 7/226 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           MK+AV + SGG DSTT    A +   ++  +  +YGQR  R E++ A+++A  LG     
Sbjct: 1   MKKAVCLLSGGMDSTTLAFVAKKMGYDILALHVNYGQRTERKELEAAKKVADVLGVVEFL 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
            + +    +   SSLT  +I V          PNT+VP RN   +++A  Y      EA+
Sbjct: 61  EISLDYFKKFGASSLTDMNIEVEAGVLGRADNPNTYVPFRNGNLISIATSYCESRDGEAI 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLG--MAKDIRFETPLMWIDKAETWALADYYGK 177
             GV   D +GYPDC   F++A+ HA+ +G    K I   TP + ++K +         +
Sbjct: 121 FIGVQSGDHTGYPDCTPRFIEAMQHAIYVGTQTEKSIELLTPFVMMNKTDILKEG---ME 177

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           L +    T +CY+    + CG C++C  R         + P     
Sbjct: 178 LCVPYEHTWSCYS-ENDEACGVCSSCLARLKAFADLGMEDPIPYKK 222


>UniRef50_Q8K986 7-cyano-7-deazaguanine synthase n=1 Tax=Buchnera aphidicola
           (Schizaphis graminum) RepID=QUEC_BUCAP
          Length = 232

 Score =  278 bits (711), Expect = 1e-73,   Method: Composition-based stats.
 Identities = 104/227 (45%), Positives = 152/227 (66%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+ +++FSGGQDSTTCL+     Y E++C+TFDY Q H++EID AR ++     + H  
Sbjct: 1   MKKILIIFSGGQDSTTCLIHYTNIYKEIYCITFDYNQLHKSEIDSARFISNYFNVKKHIF 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+  L  L++SSLT + I + D  P    +P+TFVPGRNILFLTL++IYA+  +  +++
Sbjct: 61  VDLKCLKNLSISSLTDEKISILDNHPLNFSLPSTFVPGRNILFLTLSSIYAFNHQINSIV 120

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            GV E DFSGYPDCR+ F+K +N  V +GM   I F++PL+ + KAE WAL+DY+     
Sbjct: 121 LGVNEIDFSGYPDCRNAFLKKMNDVVQIGMNCKINFQSPLINLSKAEIWALSDYWNSTQF 180

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQK 227
           + N T+TCY G +G GCG C +C LR +G N + ++    M  +K+K
Sbjct: 181 ILNNTVTCYQGIQGKGCGQCQSCILRNDGFNKWKSNPSYYMKKLKEK 227


>UniRef50_C1D6L0 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria RepID=QUEC_LARHH
          Length = 229

 Score =  277 bits (710), Expect = 2e-73,   Method: Composition-based stats.
 Identities = 92/219 (42%), Positives = 129/219 (58%), Gaps = 3/219 (1%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + A+VV SGGQDSTTCL  AL ++ +V  ++FDYGQRHR E+D AR +A   G   H ++
Sbjct: 8   EHALVVLSGGQDSTTCLYWALSRFAQVSAISFDYGQRHRVELDAARTIAAMAGV-GHTII 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            +   + L  ++LT   +           +PNTFVPGRN++FLT AA +A+      ++T
Sbjct: 67  PINTFSALGGNALTDQQMSPDTGPDAETLLPNTFVPGRNLVFLTFAAAWAWPRGIRHIVT 126

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GV +TD+SGYPDCR+  ++AL  A++LGM   +R   PLM++ KA+T  LA   G +   
Sbjct: 127 GVAQTDYSGYPDCRENTLRALELAINLGMESRMRLHMPLMFLSKADTVTLARTVGAMPA- 185

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
              + TCY G     CG CAAC LRA G        P +
Sbjct: 186 LAFSHTCYAGA-VPPCGQCAACVLRAKGFAEAGIPDPLL 223


>UniRef50_B8GJL3 7-cyano-7-deazaguanine synthase n=6 Tax=Euryarchaeota
           RepID=QUEC_METPE
          Length = 221

 Score =  277 bits (709), Expect = 2e-73,   Method: Composition-based stats.
 Identities = 67/222 (30%), Positives = 99/222 (44%), Gaps = 7/222 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +AV + SGG DS+T    A      +H +  +YGQR  + E+  A  +A  L A     L
Sbjct: 2   KAVCLLSGGMDSSTLAYVAKDMGYHIHALHLNYGQRTEKKELQSATAIARSLSAEEFIPL 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+        SSLT  +I V  ++     +PNT+VP RN   L++A  +A    A+A+  
Sbjct: 62  DLGYFKAFGKSSLTDPAIDVDVFDENRPELPNTYVPFRNANLLSIATSFAESRDADAIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKLD 179
           GV   D+SGYPDCR  F++A    + LG      I   TP + + K +          L 
Sbjct: 122 GVQALDYSGYPDCRPAFIQAFQQVMDLGTADGTHIDLLTPFIGLTKTDILRKGLD---LG 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           +    T +CY   +   CG C +C+ R           P   
Sbjct: 179 VPYEHTWSCYQ-NEEKACGVCGSCHFRQKAFAELGLSDPIPY 219


>UniRef50_Q8YM45 7-cyano-7-deazaguanine synthase n=14 Tax=cellular organisms
           RepID=QUEC_ANASP
          Length = 229

 Score =  276 bits (707), Expect = 3e-73,   Method: Composition-based stats.
 Identities = 80/231 (34%), Positives = 118/231 (51%), Gaps = 13/231 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV++ SGG DS+T L QA     E + ++FDY QRHR E+  A  +A   G   H+V++
Sbjct: 2   KAVILLSGGLDSSTILYQAKADGCECYSISFDYQQRHRRELHSAFLVAQTAGIVQHQVIN 61

Query: 63  VTLLNELAVSSLTRDSIPVP---DYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
              L     S+LT D+I +P     +  +  IP T+VP RN +FL+ A  YA  + A+ V
Sbjct: 62  FD-LRLWGGSALTDDNIDLPQERSLDAMSQNIPVTYVPARNTIFLSFALAYAEAIAAQRV 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADY 174
             GV   D+SGYPDCR ++++A+     LG       + I    PL+ + K E   L + 
Sbjct: 121 YIGVNALDYSGYPDCRPDYIEAMQEVFRLGTKQGREGQPINIVAPLINLKKTEIIQLGN- 179

Query: 175 YGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
             +L +  N T +CY G     CG C +C LR         + P   A +K
Sbjct: 180 --QLGVPWNLTWSCYAGGDV-ACGVCDSCRLRLAAFAELGLEDPLPYAYLK 227


>UniRef50_A8EWF5 7-cyano-7-deazaguanine synthase n=27 Tax=Epsilonproteobacteria
           RepID=QUEC_ARCB4
          Length = 228

 Score =  275 bits (705), Expect = 7e-73,   Method: Composition-based stats.
 Identities = 75/224 (33%), Positives = 115/224 (51%), Gaps = 8/224 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKV 60
           K+A+ + SGG DST     A +   E+  V F+YGQR    E+   R++  +L  ++   
Sbjct: 7   KKAICILSGGMDSTLSSYIAKKDGFEIIAVHFNYGQRTQNRELKAFRDICNELDIKSKYE 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+    ++  ++LT  SI VP    EA G+P T+VP RN +FL + A  A +  A A+ 
Sbjct: 67  IDIPFFTQIGANALTDMSIDVPTGGLEA-GVPITYVPFRNGIFLAITAAIAEKEGATAMY 125

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKL 178
            GV + D SGYPDC +EF++ +  A++ G      I   TPL+ + KA+    A    KL
Sbjct: 126 IGVVQEDSSGYPDCTEEFIQDMKKAINQGTKEDTKIEIITPLVHLSKAQIVQEA---IKL 182

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           ++    T +CY   + + CG C +C LR NG        P    
Sbjct: 183 NVPLELTWSCYK-EEEEACGVCDSCRLRLNGFEQAGKKDPIKYK 225


>UniRef50_A3ZMY4 Predicted PP-loop superfamily ATPase n=1 Tax=Blastopirellula marina
           DSM 3645 RepID=A3ZMY4_9PLAN
          Length = 219

 Score =  274 bits (702), Expect = 1e-72,   Method: Composition-based stats.
 Identities = 71/224 (31%), Positives = 106/224 (47%), Gaps = 6/224 (2%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M + V V SGG DS T L   +    +V  ++ +YGQRH  E+D AR+L  K+G     V
Sbjct: 1   MSKVVAVVSGGMDSATLLYHLIDAGHDVRAISINYGQRHVKELDYARQLCDKVGV-PQIV 59

Query: 61  LDVTLLN-ELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
            D++ +N     +SL+   I +P+     + +  T VP RN++ L+++  +A   +  AV
Sbjct: 60  ADLSAINPLFGANSLSGREIEIPEGHYAEENMKLTVVPNRNMILLSVSIAWAISQECVAV 119

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G    D + YPDCR EF  A++ A  L     +    P + IDK +    A    +L 
Sbjct: 120 AYGAHSGDHAIYPDCRPEFAAAMDQAAKLCDWSAVELWRPFVDIDKGDI---AQRGSELG 176

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           +    T TCY G   + CG C AC  R      +    PT   A
Sbjct: 177 VPYELTWTCYKGL-AEHCGKCGACVERKEAFAKHGLVDPTAYMA 219


>UniRef50_Q1QCP3 7-cyano-7-deazaguanine synthase n=122 Tax=Bacteria RepID=QUEC_PSYCK
          Length = 259

 Score =  274 bits (701), Expect = 2e-72,   Method: Composition-based stats.
 Identities = 86/239 (35%), Positives = 122/239 (51%), Gaps = 22/239 (9%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + AVV+ SGG DS TCL  A  +Y  V  V+FDYGQRH +E+  A+ +A   G   H+++
Sbjct: 26  QNAVVLLSGGLDSVTCLYWAKARYASVTAVSFDYGQRHNSELVAAKAIAETAGV-NHRII 84

Query: 62  DVTLLNELAVSSLTRDSIPVPDY-----------EPEADGIPNTFVPGRNILFLTLAAIY 110
           D+  + +L  SSLT  S+ VPD            E + D IPNT+VP RN +FL+ A   
Sbjct: 85  DID-IAQLGGSSLTDHSMIVPDGDTDKFPDKKRDEIDNDAIPNTYVPARNTIFLSYALAV 143

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR-----FETPLMWIDK 165
           A    +  ++ GV   D+SGYPDCR E++ A  H  +L     +       +TPL  + K
Sbjct: 144 AEVTDSNHIVIGVSSVDYSGYPDCRPEYIAAFQHMANLATKAGVTGHHLSIQTPLQQLSK 203

Query: 166 AETWALADYYGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           A+T  L      L +   +T++CY     G  CG C +C LR  G        PT   +
Sbjct: 204 AKTIELGLS---LGVDYGQTISCYQADANGLACGVCDSCALRRQGFAQAGVADPTHYQS 259


>UniRef50_A8GPK3 7-cyano-7-deazaguanine synthase n=18 Tax=Proteobacteria
           RepID=QUEC_RICAH
          Length = 227

 Score =  273 bits (698), Expect = 4e-72,   Method: Composition-based stats.
 Identities = 76/230 (33%), Positives = 119/230 (51%), Gaps = 13/230 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AV++ SGG DS T L  A +   E+H ++F+YGQR+ AE+   +EL  +   + HK+
Sbjct: 1   MKKAVMLVSGGADSATVLAMAREIGYEIHSMSFNYGQRNNAELRKVKELIKEYNVKQHKI 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEA---DGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +D+  L     S+LT D+I VP Y       + +P T+VP RN +FL+ A  YA  + A+
Sbjct: 61  VDID-LRAFGGSALTDDNIDVPHYHDVNAVPEDVPVTYVPSRNTIFLSYALGYAEVIGAK 119

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALA 172
            +  GV  +D + YPDCR E++K+     +L        + I   TPL+ + K +     
Sbjct: 120 DIFIGVHTSDSANYPDCRPEYIKSFEKMANLATSIGIQGQKITIHTPLIDMTKEQIIRTG 179

Query: 173 DYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVM 221
               KL +    T++CY   + D  CG+C AC +R +         P   
Sbjct: 180 ---LKLGVDYKNTISCYEPTEDDSSCGNCLACMIRLDAFKKNNIQDPIKY 226


>UniRef50_B0S9S9 7-cyano-7-deazaguanine synthase n=13 Tax=Bacteria RepID=QUEC_LEPBA
          Length = 248

 Score =  271 bits (693), Expect = 1e-71,   Method: Composition-based stats.
 Identities = 79/238 (33%), Positives = 115/238 (48%), Gaps = 24/238 (10%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD-------EVHCVTFDYGQRHRAEIDVARELALKLG 54
           K A+V+ SGG DSTTCL  A + +         +  ++FDY Q+H+ E+  ++++A  LG
Sbjct: 16  KGAIVLLSGGLDSTTCLYLAAKDFGYPKNKKLPLLALSFDYSQKHKIELTKSKKIAKTLG 75

Query: 55  ARAHKVLDVTLLNELAVSSLTRDSIPVPD-----YEPEADGIPNTFVPGRNILFLTLAAI 109
            +   V+          SSLT   I V       +  +   IPNT+VPGRNILFL+ A  
Sbjct: 76  IK--HVIQKLDPGFFLGSSLTESKIKVRKNAKSLFSGDETEIPNTYVPGRNILFLSFALS 133

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD-------IRFETPLMW 162
            A     +++  GV   D+SGYPDCR EF+ +     +LG  K        I+ +TPL+ 
Sbjct: 134 LAEGHGYDSIYIGVNALDYSGYPDCRPEFIDSFQKMANLGTKKGVSGTGDSIQIKTPLLH 193

Query: 163 IDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
           + K E   L      +      T +CY+   G  CG C +C LRA G        P +
Sbjct: 194 LGKKEIIELGMS---VGAPLGLTHSCYDPVSGKPCGKCDSCILRAKGFLEVGLKDPAL 248


>UniRef50_B2I4Y4 7-cyano-7-deazaguanine synthase n=16 Tax=Bacteria RepID=QUEC_XYLF2
          Length = 230

 Score =  271 bits (693), Expect = 2e-71,   Method: Composition-based stats.
 Identities = 83/230 (36%), Positives = 114/230 (49%), Gaps = 13/230 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AV++ SGG DS      A  Q   VH ++  YGQRH +E+D A  +A  L   AHKV
Sbjct: 1   MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKV 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +DV  L  +  S+LT D I +PD     +GIP T+VP RN + L+LA  +A  + A  + 
Sbjct: 61  VDVD-LRSIGGSALTDD-IEIPDA--GGEGIPVTYVPARNTIMLSLALGWAEVIGAADIF 116

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADYY 175
            GV   D+SGYPDCR +F+ A     +L          +    PL ++ KAE       +
Sbjct: 117 CGVNAVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAPLQFLSKAEIVHEGLLH 176

Query: 176 GKLDLVRNETLTCYNG-FKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           G   +    T++CY     G  CG C AC LR  G        PT    +
Sbjct: 177 G---VDFGLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMEL 223


>UniRef50_C8R2H0 ExsB protein n=1 Tax=Desulfurivibrio alkaliphilus AHT2
           RepID=C8R2H0_9DELT
          Length = 258

 Score =  269 bits (687), Expect = 7e-71,   Method: Composition-based stats.
 Identities = 87/245 (35%), Positives = 122/245 (49%), Gaps = 25/245 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVV+ SGG DSTT L  A       HC++F YGQRH  E+++AR  A   GA  H +L++
Sbjct: 18  AVVLLSGGLDSTTVLALAKAAGYRCHCLSFRYGQRHTQELELARANARHWGAARHLILNI 77

Query: 64  TLLNELAVSSLTRDSIPVP---------------DYEPEADGIPNTFVPGRNILFLTLAA 108
             L+ +  S+LT D + VP               D    A  +P T+VPGRN +FL  AA
Sbjct: 78  E-LDAIGGSALTGDELEVPKDNLSPLDSPDGAETDAPGMAGEVPVTYVPGRNTIFLAYAA 136

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWI 163
            +A  + A  +  GV   DFSGYPDCR ++++AL  A++LG       +  R + PL+ +
Sbjct: 137 SWAEVLGARDIFIGVNAVDFSGYPDCRPDYLEALAAALNLGSQCGREGRPFRLQAPLLHL 196

Query: 164 DKAETWALADYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVMA 222
            KAE  A       L +    T +CY+  +    CG C AC LR  G        PT   
Sbjct: 197 SKAEIIAKGLA---LGVDYARTHSCYDPDQQHRACGRCDACILRRRGFAAAGCPDPTTYQ 253

Query: 223 AMKQK 227
             + +
Sbjct: 254 PARSE 258


>UniRef50_B3QPB3 7-cyano-7-deazaguanine synthase n=4 Tax=cellular organisms
           RepID=QUEC_CHLP8
          Length = 227

 Score =  269 bits (687), Expect = 8e-71,   Method: Composition-based stats.
 Identities = 67/222 (30%), Positives = 100/222 (45%), Gaps = 7/222 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +AV++ SGG DS      A     E+  +  +YGQR  + E++  R++    G      +
Sbjct: 2   KAVLLVSGGMDSLVATALAHHHGFELAAMHVNYGQRTWQKELECFRQICDHYGIEKRLEI 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D   L  +  SSLT  +IPV   +     IP ++VP RN  FL++A  ++  + A  +  
Sbjct: 62  DAGFLGAIGGSSLTDAAIPVGPADLAGTDIPTSYVPFRNANFLSMAVSWSEVIGANRIYI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKD--IRFETPLMWIDKAETWALADYYGKLD 179
           G  E D SGYPDCR  F  A N  +  G   +  I   TPL+ + KAE         +LD
Sbjct: 122 GAVEEDSSGYPDCRKVFYDAFNQVIEHGTRPETSIEIVTPLIDMSKAEIVKRG---MELD 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              + + +CY   +G  CG C +C  R           P   
Sbjct: 179 APFHFSWSCYK-SEGKACGVCDSCARRLRAFASVGIADPVEY 219


>UniRef50_Q1QVW9 7-cyano-7-deazaguanine synthase n=23 Tax=Proteobacteria
           RepID=QUEC_CHRSD
          Length = 251

 Score =  269 bits (687), Expect = 8e-71,   Method: Composition-based stats.
 Identities = 77/223 (34%), Positives = 115/223 (51%), Gaps = 6/223 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AVV++SGG DS T L +AL+   EVH ++F YGQRH  E++ A ++  +LG  AH+V+D
Sbjct: 30  KAVVIYSGGMDSYTVLHRALRAGFEVHALSFHYGQRHSRELETAHDVCQRLGI-AHQVVD 88

Query: 63  VTLL-NELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +  +   +  S+LT  +  +PD + +AD +  T VP RN++ L+LA  +A  + A     
Sbjct: 89  IRAIHGLIGNSALTDATQTMPDGDYDADNMAATVVPNRNMILLSLAIGHAVNIGANVCFY 148

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G    D   YPDCR EFV+ +N   ++     +R   P +   K E  A       + L 
Sbjct: 149 GAHGGDHVLYPDCRPEFVERMNDVAAIADFTPVRIAAPYLHASKEEILADGLA---MGLD 205

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
             +T TCY G +   CGHC +C  R           P V A  
Sbjct: 206 YAQTWTCYLGAE-RSCGHCGSCRERLAAFAAQGVTDPLVYAGA 247


>UniRef50_B9Z9V1 ExsB protein n=2 Tax=Halobacteriaceae RepID=B9Z9V1_NATMA
          Length = 251

 Score =  268 bits (685), Expect = 1e-70,   Method: Composition-based stats.
 Identities = 75/223 (33%), Positives = 107/223 (47%), Gaps = 7/223 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           RAVV+ SGG DS T   +A  Q  +++ +   YGQR    E++ AR+LA +  A     +
Sbjct: 24  RAVVLLSGGMDSATAAAEARSQGYDIYALHTSYGQRTETRELECARQLADEFEANDFLQI 83

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +   L+ +  SSLT D + V D + ++D IP+++VP RN   L +A  YA     EAV  
Sbjct: 84  ETGHLSAIGASSLTDDEMAVADADMDSDEIPSSYVPFRNANLLAMAVSYAEANDCEAVFI 143

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKLD 179
           G    DFSGYPDCR EF +A      +G     +I  E P +   K +    A +  +L 
Sbjct: 144 GAHSEDFSGYPDCRPEFFEAFERVADVGTKPETEISIEAPFVEWSKTDI---AAHGTELG 200

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           +    T +CY   +   CG C AC  R           P   A
Sbjct: 201 VPYEHTWSCYRDDQ-PACGTCDACAYRLQAFQRIGVRDPIEYA 242


>UniRef50_B8HQ99 7-cyano-7-deazaguanine synthase n=1 Tax=Cyanothece sp. PCC 7425
           RepID=QUEC_CYAP4
          Length = 229

 Score =  268 bits (685), Expect = 1e-70,   Method: Composition-based stats.
 Identities = 72/231 (31%), Positives = 114/231 (49%), Gaps = 16/231 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V++ SGG DSTT L  A     + + ++F+YGQRH+ E+  A  +A   G   H+V+ 
Sbjct: 2   KIVILLSGGLDSTTVLYLARSLGHDCYALSFNYGQRHKQELAAAIAVAQLAGVIQHQVIS 61

Query: 63  VTLLNELAVSSLTRDSIPVPD---YEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
              L     S+LT  ++ +P    ++  A+ IP T+VP RN +FL+ A  YA  + A  V
Sbjct: 62  FD-LGLWGGSALTDYTLDLPQNRAFKEMAEQIPITYVPARNTIFLSFALSYAEAIGATQV 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADY 174
             G+   D+SGYPDCR +++ A+     LG       + I+  TPL+ + K     L + 
Sbjct: 121 YAGMNAIDYSGYPDCRPDYIAAMQEVYRLGTKQGREGEPIQIITPLIDLTKPAIIQLGN- 179

Query: 175 YGKLDLVRNETLTCYNGFKGD----GCGHCAACNLRANGLNHYLADKPTVM 221
             +L +   +T +CY+   G+     CG C +C LR           P   
Sbjct: 180 --RLGVPWAKTWSCYSDGGGNDPPVACGVCDSCRLRLAAFAELSLTDPLPY 228


>UniRef50_Q55468 7-cyano-7-deazaguanine synthase n=43 Tax=Bacteria RepID=QUEC_SYNY3
          Length = 232

 Score =  268 bits (685), Expect = 1e-70,   Method: Composition-based stats.
 Identities = 74/220 (33%), Positives = 109/220 (49%), Gaps = 10/220 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K AVV+ SGG DS T    A ++   V  ++F+YGQRH  E+  A ++   LG   H  +
Sbjct: 3   KTAVVLLSGGLDSATVAAIAKREGYRVIALSFNYGQRHDRELRAAADIVQALGIPEHFSI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++  L +   SSLT     +P    E D IP+T+VPGRN +F+ L    A    AEAV  
Sbjct: 63  NLD-LAQWGGSSLTDRQQTLPQTGVEPDIIPSTYVPGRNTVFIALGLSLAEAKGAEAVFL 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADYYG 176
           G+   D+SGYPDCR E++       +L        + I+   PL+ + K +    A    
Sbjct: 122 GINAIDYSGYPDCRPEYLATYQQLAALSSKVGVEGRPIQLLAPLIELSKVDIVHKA---L 178

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
           +L +   +T +CY G + + CG C +C LR   L      
Sbjct: 179 ELGVPIAQTWSCYAGGE-EPCGRCDSCRLRDQALIEAGHP 217


>UniRef50_A7HHA2 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria RepID=QUEC_ANADF
          Length = 233

 Score =  267 bits (684), Expect = 1e-70,   Method: Composition-based stats.
 Identities = 90/228 (39%), Positives = 120/228 (52%), Gaps = 12/228 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           KRAVV+ SGG DSTTCL  A +   E HC++ DYGQRH+ E+  AR L+  LGA  H+V+
Sbjct: 7   KRAVVLLSGGLDSTTCLAVARRDGFEAHCLSVDYGQRHKGELARARRLSRALGAADHRVV 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPE---ADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            V  L+    S+LT  +I VP        A  IP T+VP RN + L LA  +A  + AE 
Sbjct: 67  KVD-LSAFGGSALTDRAIAVPKGRSGRRRAAEIPVTYVPARNTVLLALALAHAETLGAED 125

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALAD 173
           V  GV   D+SGYPDCR  F++A     ++        + +R   PL+ + KA    L  
Sbjct: 126 VYVGVNAIDYSGYPDCRPAFLRAFERLATVATKAGVEGRPLRIHAPLLRLTKAGIVRLGT 185

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
               L +    TL+CY+  +G  CG C AC LR  G        PT+ 
Sbjct: 186 S---LGVPYRVTLSCYDPVRGRACGACDACVLRRKGFAEAGIPDPTLY 230


>UniRef50_O29807 7-cyano-7-deazaguanine synthase n=1 Tax=Archaeoglobus fulgidus
           RepID=QUEC_ARCFU
          Length = 239

 Score =  267 bits (683), Expect = 2e-70,   Method: Composition-based stats.
 Identities = 74/219 (33%), Positives = 108/219 (49%), Gaps = 6/219 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV++ SGG DS+T L   L    EVH +TF YGQ+H  EI+ A ++A     R  KV  
Sbjct: 2   KAVMLLSGGIDSSTLLYYLLDGGYEVHALTFFYGQKHSKEIESAEKVAKAAKVRHLKVDI 61

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
            T+ + ++  +LT +   VP      +    T VP RN++ L++AA YA ++ A+ V   
Sbjct: 62  STIHDLISYGALTGEE-EVPKAFYSEEVQRRTIVPNRNMILLSIAAGYAVKIGAKEVHYA 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLG-MAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              +D+S YPDCR EFVKAL+ AV L  +   +    P + + KA+   L     KL + 
Sbjct: 121 AHLSDYSIYPDCRKEFVKALDTAVYLANIWTPVEVRAPFVDMTKADIVRLG---LKLGVP 177

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
              T +CY G     C  C  C  R           P +
Sbjct: 178 YELTWSCYEGG-DRPCLSCGTCLERTEAFLANGVKDPLL 215


>UniRef50_B2UN88 ExsB protein n=6 Tax=Bacteria RepID=B2UN88_AKKM8
          Length = 223

 Score =  266 bits (681), Expect = 3e-70,   Method: Composition-based stats.
 Identities = 66/226 (29%), Positives = 105/226 (46%), Gaps = 7/226 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           M+ A V+ SGG DS+  L  A + +     ++FDY   H   E+  A   A  LG   H+
Sbjct: 4   META-VLLSGGLDSSVALHWAHRNHRVRVALSFDYASNHAERELKCAAWQAASLGIE-HR 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V+D++ ++    S+L   +  +PD   + + +  T VP RN +FL++AA  A    A+ +
Sbjct: 62  VIDISSISSHLKSALLSGADDIPDGSYDEELMKQTVVPFRNGIFLSIAAGVAESCGAQQL 121

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +      D S YPDCR+EF+ A+  A+ LG    +    P + + K    A+      L 
Sbjct: 122 VIAAHSGDHSIYPDCREEFMAAMTEAIRLGTYAGLGILRPFIHMSKGGIAAMGQD---LG 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
           +  + T +CY G     CG C+ C  R           PT  A  +
Sbjct: 179 VDFSRTYSCYKGG-AVHCGVCSTCMERREAFREAGIPDPTAYAPAE 223


>UniRef50_A6DLD5 ExsB protein n=1 Tax=Lentisphaera araneosa HTCC2155
           RepID=A6DLD5_9BACT
          Length = 226

 Score =  264 bits (676), Expect = 1e-69,   Method: Composition-based stats.
 Identities = 74/230 (32%), Positives = 111/230 (48%), Gaps = 12/230 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKL-GARA 57
           MK+A V+ SGG DSTT L   ++   YDEV+ ++FDYGQ+H  E+ VA+     L   + 
Sbjct: 1   MKKAAVLISGGLDSTTLLHHVIKSLGYDEVYALSFDYGQKHSRELIVAKAQCDSLPEVKE 60

Query: 58  HKVLDVTLLNELAVSS--LTRDSIPVPDYEPEADG---IPNTFVPGRNILFLTLAAIYAY 112
           H VLD++ + +   SS  L +  + VPD +  A+G    P T+VP RN++ L++AA +A 
Sbjct: 61  HNVLDISYMGDFLGSSSALVKGGVEVPDLKDIAEGDLDQPVTYVPNRNMMLLSIAASFAE 120

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 +  G    D  GY DC + F+  +N   SL     +  E P + + K     + 
Sbjct: 121 SRDCRQLYYGAQAQDEYGYWDCTESFLARMNDVFSLNRRTGVHVEAPFVNMSKGHVAKIG 180

Query: 173 DYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                L +   +T TCY G     C  C +C  RA        D P +  
Sbjct: 181 LA---LGIDYAQTWTCYRGG-DKPCEVCPSCVERALAFKECGIDDPLLKK 226


>UniRef50_C0QST3 7-cyano-7-deazaguanine synthase n=7 Tax=Aquificales
           RepID=QUEC_PERMH
          Length = 227

 Score =  263 bits (674), Expect = 2e-69,   Method: Composition-based stats.
 Identities = 76/227 (33%), Positives = 116/227 (51%), Gaps = 15/227 (6%)

Query: 1   MKRA---VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARA 57
           MK++   +++ SGG DS   L  A +++D+V+ V+FDYGQ+HR E+D A++L  + G   
Sbjct: 1   MKQSSSIIILVSGGMDSAALLWLAKKEFDKVYAVSFDYGQKHRVELDFAKKLTEEAGVEE 60

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           H + +V  L  +  S+LT  S+ +P  E   +G P T VP RN+ FL++AA +A   + E
Sbjct: 61  HFIAEVPHLKGIKGSALTDRSVEIPSEEY-PEGPPVTTVPMRNLNFLSIAASFADIYEIE 119

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGM------AKDIRFETPLMWIDKAETWAL 171
            +  GV   D S YPDCR EFV A   A++            I   TP + + K +   +
Sbjct: 120 NIGIGVHSVD-SPYPDCRAEFVSAAEAAINASSVMVAKKKNRITVYTPFLGMTKTDVLRI 178

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
                +L +   +T +CY G     CG CA C  R         + P
Sbjct: 179 G---LELGVPYEKTYSCYRGTI-PPCGECATCRQRIEAFRSVGLEDP 221


>UniRef50_A6VQW6 7-cyano-7-deazaguanine synthase n=22 Tax=Pasteurellaceae
           RepID=QUEC_ACTSZ
          Length = 235

 Score =  261 bits (668), Expect = 1e-68,   Method: Composition-based stats.
 Identities = 107/225 (47%), Positives = 144/225 (64%), Gaps = 8/225 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++AVV+FSGGQDSTTCL+QA++ Y    V  VTF YGQRH  E+  A+ +A  LG +   
Sbjct: 10  RKAVVIFSGGQDSTTCLLQAIRDYGPNNVEAVTFQYGQRHAIELQKAQWIAQDLGVKQ-T 68

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++D +++  +  +++  D+      + + +G+PNTFV GRN LFL  AAIYA       +
Sbjct: 69  LIDTSVIKTITTNAMMSDA----QIKQDENGMPNTFVDGRNALFLLYAAIYAKGQGITDI 124

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           ITGVCETDFSGYPDCR+ F+K++N  ++L M       TPLM++ KA+TW LAD  G LD
Sbjct: 125 ITGVCETDFSGYPDCRNVFIKSMNVTLNLAMDYQFNIRTPLMYLTKAQTWQLADELGALD 184

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
            VR  T TCY G +G GCG C +C LR  GL  YLA K  V  + 
Sbjct: 185 YVRQYTHTCYLGVEG-GCGTCPSCLLREKGLKQYLAQKSAVEKSS 228


>UniRef50_Q3AR02 7-cyano-7-deazaguanine synthase n=12 Tax=Bacteria RepID=QUEC_CHLCH
          Length = 228

 Score =  260 bits (666), Expect = 2e-68,   Method: Composition-based stats.
 Identities = 68/222 (30%), Positives = 103/222 (46%), Gaps = 7/222 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS      A Q   E+  +  +YGQR  + E++  R +      +    +
Sbjct: 2   KAILLLSGGMDSLVTTAIAQQAGFELAAMHVNYGQRTMQRELNSFRAICSHYSIQQRLEI 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +   L ++  SSLT  S+PV     E+  IP ++VP RN  FL++A  +A  + AE +  
Sbjct: 62  NADFLGKIGGSSLTDLSMPVSVANLESHAIPASYVPFRNAGFLSMAVSWAEVIGAERIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKLD 179
           G  E D SGYPDCR  F +A N  + LG      I   TPL+ + K E         +L 
Sbjct: 122 GAVEEDSSGYPDCRKIFYEAFNRVIELGTKPETHIEVVTPLIALKKWEIVRKG---IELH 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                + +CY   +G  CG C +C LR         + P   
Sbjct: 179 APFAFSWSCYK-NEGQACGVCDSCALRLRAFEQAGMEDPIDY 219


>UniRef50_A5A3S4 BcepGomrgp35 n=1 Tax=Burkholderia phage BcepGomr RepID=A5A3S4_9CAUD
          Length = 301

 Score =  259 bits (663), Expect = 5e-68,   Method: Composition-based stats.
 Identities = 99/231 (42%), Positives = 132/231 (57%), Gaps = 18/231 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K AV+VFSGGQDSTT L +A+ +   V  + F YGQ+H  E++ A ++    G    K++
Sbjct: 4   KTAVIVFSGGQDSTTILGKAIAEGYRVFPIAFTYGQKHAIELEQAAKIVDHFGLAGLKIV 63

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D++ L E+  S+L      V         +P +FVP RN  FLTLA  YA QV A+ + T
Sbjct: 64  DLSALGEVTTSALIGTDGDVNKPSDANPSLPASFVPNRNAAFLTLAHAYAQQVGADELWT 123

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GVC TD+SGYPDCRD F+ AL   +++G    I  +TPLM+IDKAET+ +A+  G LD+V
Sbjct: 124 GVCMTDYSGYPDCRDSFINALAATLNIGYDTSITIKTPLMYIDKAETFRMAELAGCLDVV 183

Query: 182 RNETLTCYNGFK------------------GDGCGHCAACNLRANGLNHYL 214
              + TCYNG +                  G GCG C AC LRA G   YL
Sbjct: 184 IEMSHTCYNGVRSNGEVPTNPSEGGGFFDWGHGCGECPACKLRAGGWREYL 234


>UniRef50_Q1IHK6 7-cyano-7-deazaguanine synthase n=9 Tax=Bacteria RepID=QUEC_ACIBL
          Length = 237

 Score =  259 bits (662), Expect = 6e-68,   Method: Composition-based stats.
 Identities = 78/226 (34%), Positives = 108/226 (47%), Gaps = 9/226 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           +AVVV SGG DST C   A+++Y  + +  +   YGQR    E      +A +LG +   
Sbjct: 10  KAVVVLSGGMDSTVCATLAVREYGAENIGALHVSYGQRTAAREKQAFAAVAERLGIQTRL 69

Query: 60  VLDVTLLNELAVSSLTRDSIPVPD-YEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            ++      +  S+LT  +I VPD  E     IP T+VP RN   L +A  +A  + A  
Sbjct: 70  AVETPFFRAIGGSALTDANIAVPDAGELIGHEIPVTYVPFRNAHLLAMAVSWAEVLGASK 129

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGM-AKDIRFETPLMWIDKAETWALADYYGK 177
           +  G    D SGYPDCR EF +A N AV  G  A DI   TPL+ + K E  +L     +
Sbjct: 130 IYIGAVAQDSSGYPDCRPEFYEAYNLAVRRGTKAGDIEVVTPLIALRKHEIVSLG---LE 186

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           L    + T +CY+  +   CG C +C LR        A  P   A 
Sbjct: 187 LGAPFDLTWSCYS-REDCACGVCDSCVLRLRAFEGAGAVDPVPYAP 231


>UniRef50_Q2N612 7-cyano-7-deazaguanine synthase n=56 Tax=Bacteria RepID=QUEC_ERYLH
          Length = 239

 Score =  259 bits (662), Expect = 7e-68,   Method: Composition-based stats.
 Identities = 78/223 (34%), Positives = 109/223 (48%), Gaps = 13/223 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K A ++ SGG DS      A +Q   VH +T DYGQRH+ E+  AR +A K+GA  H  +
Sbjct: 7   KAAAILLSGGLDSMVTAAIAQEQGYAVHALTIDYGQRHKVELQSARLIAAKIGAERHVEI 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +   L  L  S+LT D I VP      D IP T+VP RN++FL+L   +A    A  +  
Sbjct: 67  EAD-LRALGGSALTDD-IDVPKCGVGND-IPVTYVPARNLVFLSLTTAFAEASGARDIFI 123

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI------RFETPLMWIDKAETWALADYY 175
           GV   D+SGYPDCR EF+++     +L     +      +   PL  + KA+    A   
Sbjct: 124 GVNALDYSGYPDCRPEFIESFAVTANLATKAGVEESAPFKIHAPLQHMTKADI---AREC 180

Query: 176 GKLDLVRNETLTCYNGFKG-DGCGHCAACNLRANGLNHYLADK 217
            +L L    + +CY+   G   CG C +C LR  G        
Sbjct: 181 ARLGLESAWSWSCYDPLPGLKPCGACDSCRLRRKGFAEAGLAD 223


>UniRef50_A6LIS9 7-cyano-7-deazaguanine synthase n=88 Tax=Bacteria RepID=QUEC_PARD8
          Length = 224

 Score =  258 bits (659), Expect = 1e-67,   Method: Composition-based stats.
 Identities = 111/215 (51%), Positives = 141/215 (65%), Gaps = 3/215 (1%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A+V FSGGQDSTTCL  A + +  V  V F YGQ+H  EI+VAR++A        ++LDV
Sbjct: 7   ALVCFSGGQDSTTCLFWAKKHFSRVEAVCFTYGQKHSLEIEVARKIAADADV-PFQLLDV 65

Query: 64  TLLNEL-AVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +L+++L    SLT  SI +   +PE D  PNTFVPGRN++FLT AAI A       ++TG
Sbjct: 66  SLISQLDPNCSLTNASIEMDQEKPE-DSYPNTFVPGRNMVFLTFAAILARGKGIYHLVTG 124

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           V E D+SGYPDCRD FV++LN  ++L M +     TPLM  DK+E W L+D  G  DLVR
Sbjct: 125 VSEADYSGYPDCRDTFVRSLNVTLNLAMDEQFVIHTPLMDRDKSEVWELSDELGVFDLVR 184

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADK 217
            +TLTCYNG   +GCGHC AC LR +GL  YL  K
Sbjct: 185 TQTLTCYNGVMAEGCGHCPACKLRKDGLEKYLKRK 219


>UniRef50_B2RJS2 7-cyano-7-deazaguanine synthase n=5 Tax=Bacteria RepID=QUEC_PORG3
          Length = 220

 Score =  257 bits (658), Expect = 2e-67,   Method: Composition-based stats.
 Identities = 60/223 (26%), Positives = 96/223 (43%), Gaps = 8/223 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKV 60
           K +++V SGG DS T + +  ++      ++FDYG +H   E+  AR  A +LG   H +
Sbjct: 5   KDSLIVVSGGMDSVTLMYE--KRASIALALSFDYGSKHNARELSFARLHAERLGVE-HLI 61

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  + +   S L      +P+   + + + +T VP RN + L +AA  A       + 
Sbjct: 62  IPLDFIGQYFQSDLLLSGGDIPEGRYDEENMKSTVVPFRNGIMLAVAAGLAESRGLRRIY 121

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D + YPDCR  F++ +  AV  G    +  E P   I K +   +      L +
Sbjct: 122 IANHFGDHAIYPDCRASFIRPMTEAVRCGTTNGVLIEAPYTDITKTDIARIG---ASLGI 178

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
              ET +CY G     CG C  C  R   L+      PT    
Sbjct: 179 DYAETWSCYKGG-VFHCGVCGTCVERKEALHDAGIPDPTEYEG 220


>UniRef50_Q7NCE2 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria RepID=QUEC_GLOVI
          Length = 234

 Score =  257 bits (656), Expect = 3e-67,   Method: Composition-based stats.
 Identities = 102/226 (45%), Positives = 132/226 (58%), Gaps = 11/226 (4%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+A+VVFSGGQDSTTC   A ++YDEVH VTF+Y QRH  E++ AR +   LG   H+ 
Sbjct: 1   MKKALVVFSGGQDSTTCAALACREYDEVHAVTFEYNQRHAIELESARAVGQALGLTGHEF 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEAD---GIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           + +  L     S L  D+ P+  Y   A+   G+  TFVPGRNILFLTLAA  A+ +   
Sbjct: 61  IRLGPL-LKGTSPLVSDA-PLGQYASAAELPAGVEPTFVPGRNILFLTLAANRAFCLGTG 118

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD---IRFETPLMWIDKAETWALADY 174
            ++ GVCE DF+GY DCR  FV+A+  A+  G+  D   IR  TPLM + KAET  L+  
Sbjct: 119 DIVIGVCEADFAGYWDCRQVFVEAMARALGEGIYGDANAIRIHTPLMRLTKAETVKLSVE 178

Query: 175 YG--KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
               + + V   + TCY G +G GCG C AC LR  G        P
Sbjct: 179 VLGERFEEVLALSHTCYAGVRG-GCGRCHACILRDRGFREAGVPDP 223


>UniRef50_D0MDN1 ExsB protein n=1 Tax=Rhodothermus marinus DSM 4252
           RepID=D0MDN1_RHOM4
          Length = 238

 Score =  257 bits (656), Expect = 3e-67,   Method: Composition-based stats.
 Identities = 96/221 (43%), Positives = 126/221 (57%), Gaps = 9/221 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + A+V+ SGGQDSTTCL  AL  + +V  + F YGQ+H  E++ AR++A + G     VL
Sbjct: 17  ETALVLLSGGQDSTTCLYWALHYFPKVEALGFHYGQKHAVELEQARKIADRAGV-PFTVL 75

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+     L  S+LT     V    P A  +P  FVPGRN LFLTLAA Y +      ++ 
Sbjct: 76  DLR--GLLRGSALTEHEQDVSAAHPLAPHLPAAFVPGRNALFLTLAASYGFTRGIHDLVG 133

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+C+TD+SGYPDCR EF+ A+  A+SL +   IR  TPLM + KAETW LA   G LD++
Sbjct: 134 GMCQTDYSGYPDCRREFIDAMERALSLALDTPIRIHTPLMHLTKAETWRLARELGILDVI 193

Query: 182 RNETLTCYNGFKGD----GCGHC--AACNLRANGLNHYLAD 216
              T T YNG + +    G G     A  LRA G     A 
Sbjct: 194 VELTHTDYNGDRSERHPWGYGRLDNPASILRARGYEEAKAR 234


>UniRef50_Q7M8H4 7-cyano-7-deazaguanine synthase n=2 Tax=Campylobacterales
           RepID=QUEC_WOLSU
          Length = 233

 Score =  256 bits (654), Expect = 6e-67,   Method: Composition-based stats.
 Identities = 99/220 (45%), Positives = 130/220 (59%), Gaps = 8/220 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
            A+VVFSGGQDSTTCL  A++ +  V  +TFDYGQRHR EI+ +R +A KLG +   +L+
Sbjct: 13  HALVVFSGGQDSTTCLGWAIKHFKSVEAITFDYGQRHRIEIEQSRLIAQKLGIKQF-ILE 71

Query: 63  VTLLNELAVSSLTRDSIPVP-DYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
             L ++L  S+L   S  +          +P +FVP RN LF TLA  YA +     +IT
Sbjct: 72  FDLFSKLGDSALLETSSDLKASPHRTKPHLPASFVPNRNALFFTLAHAYAQREGLTHIIT 131

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+ E D+SGYPDCR+EF+  L   ++LG    I F  PL+  +KA+T+ALA   G LDLV
Sbjct: 132 GISEADYSGYPDCREEFITHLERTLNLGSDSSILFAYPLLHRNKAQTFALAKECGVLDLV 191

Query: 182 RNETLTCYNGFKGD------GCGHCAACNLRANGLNHYLA 215
             E+ TCYNG +        GC  C AC LR  G   Y A
Sbjct: 192 LEESHTCYNGDRSHRYEWGYGCKECPACGLREKGWREYQA 231


>UniRef50_Q6ARX9 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria RepID=QUEC_DESPS
          Length = 219

 Score =  255 bits (652), Expect = 9e-67,   Method: Composition-based stats.
 Identities = 74/222 (33%), Positives = 106/222 (47%), Gaps = 6/222 (2%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHK 59
           M  +++V SGG DS   L Q L++      ++FDYG +H   E+ +A  L  +   + H+
Sbjct: 1   MVDSIIVLSGGMDSAVLLAQTLKEGKTAQAISFDYGSKHNDRELPMAVGLCEEFAIK-HQ 59

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++ +  + EL  SSL      +PD     + + +T VP RN + L +AA YA  V+A  V
Sbjct: 60  IIKLPFIGELFSSSLLTGGEEIPDGAYGQENMKSTVVPFRNGIMLAIAAGYAESVQAGEV 119

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           + G    D   YPDCR EF  A   AV+LG    +    P   + K +     D    LD
Sbjct: 120 LLGSHSGDHHIYPDCRPEFNNAFAEAVNLGTDGQVAIRFPFSEMTKRDI---GDLGRTLD 176

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           L   +T TCY G +   CG CAAC+ R   L H      T  
Sbjct: 177 LDFAKTWTCYKGGE-LHCGVCAACDERKEALRHPEGLDVTKY 217


>UniRef50_C1YMB1 PreQ(0) biosynthesis protein QueC n=1 Tax=Nocardiopsis dassonvillei
           subsp. dassonvillei DSM 43111 RepID=C1YMB1_NOCDA
          Length = 243

 Score =  254 bits (650), Expect = 1e-66,   Method: Composition-based stats.
 Identities = 76/220 (34%), Positives = 95/220 (43%), Gaps = 6/220 (2%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
            VVVFSGG DSTT L         +  VT +YGQRH  E+D AR +A   GA  H +   
Sbjct: 18  VVVVFSGGMDSTTLLAHFAALRFRLVAVTVEYGQRHGKEVDAARAIARHYGAEHHILGIA 77

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
                L  S+LT   + VPD     + + +T VP RN +   LAA  A   +A  V  G+
Sbjct: 78  GFGPLLRGSALTDARVEVPDGHYAEESMRDTVVPNRNAVLANLAASVAVARRAGTVALGM 137

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAK--DIRFETPLMWIDKAETWALADYYGKLDLV 181
              D   YPDCR  FV AL H V +        R E P M   KA+    A +  +L   
Sbjct: 138 HSGDHFVYPDCRPAFVDALRHLVGVANEGFAAPRVEAPFMAWSKADI---ARHGVRLGAP 194

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              + +CY G     CG C  C  R           PT  
Sbjct: 195 LEHSWSCYKGGDIH-CGTCGTCYERREAFQEAGLPDPTEY 233


>UniRef50_B6CWJ6 ToyM n=3 Tax=Bacteria RepID=B6CWJ6_STRRM
          Length = 238

 Score =  254 bits (650), Expect = 2e-66,   Method: Composition-based stats.
 Identities = 76/221 (34%), Positives = 102/221 (46%), Gaps = 8/221 (3%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVVV SGG DSTT L         +  VT DYGQRHR EID AR +A   GA  H V+D+
Sbjct: 13  AVVVLSGGMDSTTLLAHYAMLRYRLTAVTVDYGQRHRREIDAARTIAGHYGAE-HHVVDL 71

Query: 64  TLLN-ELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           + +   L  S+LT   + VP      + +  T VP RN +   +A   A   +A  V  G
Sbjct: 72  SGIGVLLGGSALTDSEVDVPSGHYAEESMRATVVPNRNAILANVAVGAAVARQAGIVALG 131

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAK--DIRFETPLMWIDKAETWALADYYGKLDL 180
           +   D   YPDCR  FV+AL+  V +        R E P +   K +    A Y  +L+ 
Sbjct: 132 MHAGDHFVYPDCRPAFVEALDGLVRVANEGFATPRVEAPFIHWSKTDI---ARYGSRLEA 188

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
             +++ +CY G +   CG C  C  R           PT  
Sbjct: 189 PLDKSWSCYRGGE-LHCGTCGTCYERREAFRDADVPDPTEY 228


>UniRef50_C0CIL2 Putative uncharacterized protein n=1 Tax=Blautia hydrogenotrophica
           DSM 10507 RepID=C0CIL2_9FIRM
          Length = 231

 Score =  253 bits (646), Expect = 4e-66,   Method: Composition-based stats.
 Identities = 70/229 (30%), Positives = 111/229 (48%), Gaps = 14/229 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++A+V+FSGG DSTTCL  A+++Y    V  ++  YGQ+H  E++ AR +    G    +
Sbjct: 3   EKAMVLFSGGVDSTTCLGLAIEKYGVQNVIPLSVQYGQKHEKEVEAARRILEYYGIEGME 62

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEP-----EADGIP-NTFVPGRNILFLTLAAIYAYQ 113
           +    +      S L+     +P+        E +G+P +T+VP RN LFL  AA  A  
Sbjct: 63  LDLTPIFAYSDCSLLSHSEKEIPEASYAEQLRENEGVPVSTYVPFRNGLFLACAASIALS 122

Query: 114 VKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
            +   +  G    D +G  YPDC + F +++N AV  G  + IR E P + ++KA+  A 
Sbjct: 123 RECSCIYYGAHRDDAAGSAYPDCSESFFESMNRAVYEGSGQQIRIEAPFIKMNKAQIVAE 182

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
                +L +    T +CY G +   CG C  C  R+        + P  
Sbjct: 183 G---LRLKVPYEFTWSCYEGKE-KPCGVCGTCIDRSEAFKVNGVEDPAY 227


>UniRef50_Q1CTN3 7-cyano-7-deazaguanine synthase n=12 Tax=Helicobacter
           RepID=QUEC_HELPH
          Length = 228

 Score =  249 bits (637), Expect = 4e-65,   Method: Composition-based stats.
 Identities = 85/216 (39%), Positives = 133/216 (61%), Gaps = 8/216 (3%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           VV FSGGQDSTT  V A +++ +V+ V FDY Q+H  E++ A+++A  L    ++++ + 
Sbjct: 7   VVSFSGGQDSTTLAVWAKKRFKKVYLVGFDYAQKHSVELECAQKIASLLQL-PYEIISLD 65

Query: 65  LLNELAVSSLTRDSIPVPDY-EPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            L  +  S+L ++S  +  +   +   +PN+FVP RN +F+TL   YA ++ A  +  GV
Sbjct: 66  FLENITRSALFKNSNDLMGHSHVQNKDLPNSFVPNRNAIFITLLHSYAQKIGASNIALGV 125

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
            + DFSGYPDC+++F+K++ HA++LG    I+  TPLM+++KA+ + +A   G LDLV  
Sbjct: 126 SQADFSGYPDCKEDFIKSIEHALNLGSNTAIKILTPLMFLNKAQEFQMAKDLGVLDLVIK 185

Query: 184 ETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
           ET TCY G +      G GC  C AC LR  G   +
Sbjct: 186 ETHTCYQGERKILHAYGYGCNECPACQLRKKGYEEF 221


>UniRef50_A5ULR3 7-cyano-7-deazaguanine synthase n=5 Tax=Methanobacteriaceae
           RepID=QUEC_METS3
          Length = 227

 Score =  249 bits (636), Expect = 6e-65,   Method: Composition-based stats.
 Identities = 67/228 (29%), Positives = 119/228 (52%), Gaps = 14/228 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           K+A+ V SGG DS        + Y E+H +TF+YGQ+  + E+  + E+  K+G   H+V
Sbjct: 4   KKAITVLSGGLDSCVATCVYAEDY-EIHGITFNYGQKSFKQELKASEEICKKMGF-THEV 61

Query: 61  LDVTLLNELAVSSLTRDSI-------PVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           +D+  LN+++ SSLT D+         + D +   D   + +VPGRNI+F ++A  YA  
Sbjct: 62  IDLPWLNKISNSSLTSDNEIPTLTHEELDDLDICEDTASSVWVPGRNIVFTSIATSYAES 121

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           + AE +I G  + + + +PD   EF+++ N+ +++G  ++I  + P + ++K E   L D
Sbjct: 122 IGAEIIIVGWDKEEAATFPDNSKEFLESFNNMLNVGSPQNIEIKAPAIDLNKDEIVKLGD 181

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              ++      + +CY G +   CG C +C  R           P+  
Sbjct: 182 ---EIKAPMELSYSCYTGHE-KHCGVCESCMRRKRAFKLADVKDPSEY 225


>UniRef50_B9L187 ExsB protein n=1 Tax=Thermomicrobium roseum DSM 5159
           RepID=B9L187_THERP
          Length = 226

 Score =  249 bits (636), Expect = 6e-65,   Method: Composition-based stats.
 Identities = 77/226 (34%), Positives = 111/226 (49%), Gaps = 14/226 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +RAVV+ SGG DSTT           ++ ++F YGQRH  E++ AR +A KL    H+V+
Sbjct: 8   QRAVVLLSGGLDSTTLAYLLDAYGFVLYGLSFRYGQRHERELEAARAIAEKLRFAEHRVV 67

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            +  L +   SSL      +P       GIP+T+VP RN++FL +A  YA  + A+AV  
Sbjct: 68  TID-LAQWGGSSLVGSG-EIP--RQPTSGIPSTYVPVRNLVFLAIAHAYAEAIDADAVAI 123

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLG------MAKDIRFETPLMWIDKAETWALADYY 175
            V + D+SGYPDCR EF+ A   A  L         + I    P + + K+    L    
Sbjct: 124 AVSQVDYSGYPDCRGEFLAAYQQAADLASRRFVETGRRIPVLAPFLDVPKSAIVRLG--- 180

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            +L +   ET +CY G +   C  C +C LRA          P + 
Sbjct: 181 LRLGVDYAETWSCYRGGE-RPCQECDSCRLRAQAFAEAGVPDPLLQ 225


>UniRef50_C4Z7K4 Queuosine biosynthesis protein QueC n=5 Tax=Bacteria
           RepID=C4Z7K4_EUBE2
          Length = 227

 Score =  248 bits (635), Expect = 7e-65,   Method: Composition-based stats.
 Identities = 70/231 (30%), Positives = 105/231 (45%), Gaps = 17/231 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +A+V+ SGG DSTT L  A+ +Y  D V  ++  YGQ+H  EI  A+ ++   G      
Sbjct: 2   KALVLSSGGVDSTTALALAVSRYGKDNVIALSVSYGQKHDKEIQAAKAVSAFYGVEQ-LF 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYE--------PEADGIPNTFVPGRNILFLTLAAIYAY 112
           LD++ + + +  SL + S      E         E +   +T+VP RN LFL+ AA  A 
Sbjct: 61  LDLSKIFQYSNCSLLQQSTEEIPEESYARQIEKTEGEKPVSTYVPFRNGLFLSSAASIAL 120

Query: 113 QVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
                 ++ G    D +G  YPDC   F  A+N A+  G    ++ E P + + KAE   
Sbjct: 121 SKDCSVILYGAHADDSAGFAYPDCSPMFNDAMNQAIFEGSGHQLKVEAPFVNMTKAEVVK 180

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           +     +L+     T +CY G K   CG C  C  RA          P + 
Sbjct: 181 MG---LELNAPYELTWSCYEG-KDVPCGKCGTCIDRAAAFAANGVSDPAIK 227


>UniRef50_D2RGV6 ExsB protein n=2 Tax=Archaeoglobaceae RepID=D2RGV6_ARCPR
          Length = 233

 Score =  248 bits (634), Expect = 1e-64,   Method: Composition-based stats.
 Identities = 66/217 (30%), Positives = 105/217 (48%), Gaps = 8/217 (3%)

Query: 8   FSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARA---HKVLDVT 64
            SGG DS+T L   +++YDE++ +T+ YGQRH  E++ A+ +  K   +A   H++ D++
Sbjct: 1   MSGGIDSSTLLWYLMKRYDEIYVITYIYGQRHAREVEHAKMIVEKAREKAKIHHEIADIS 60

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
            +  L        +  VP+     +    T VP RN++ L +A  YA +V    V     
Sbjct: 61  CIQRLIAKGALTGNEEVPEGFYTDESQKKTIVPNRNMILLAIAVGYAVKVGEREVYYAAH 120

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGM-AKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
           ++D++ YPDCR EFVKAL+ AV L      +  + P + + KA+   L     KL +   
Sbjct: 121 KSDYATYPDCRKEFVKALDTAVYLATIFDPVVIKAPFVDMTKADIVKLG---LKLGVPYE 177

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
            T +CY G +   C  C  C  R           P +
Sbjct: 178 LTWSCYRG-EERPCLRCGTCLERTEAFLINGVKDPAL 213


>UniRef50_A6UV33 7-cyano-7-deazaguanine synthase n=12 Tax=Methanococcales
           RepID=QUEC_META3
          Length = 234

 Score =  248 bits (634), Expect = 1e-64,   Method: Composition-based stats.
 Identities = 64/232 (27%), Positives = 115/232 (49%), Gaps = 14/232 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           +AV V SGG DS  C+  A  +  E++ +TFDYGQR  + EI  +++L+   GA  HKV+
Sbjct: 2   KAVCVLSGGLDSAVCMAMAKSEGLELYSITFDYGQRAVKKEIIASKKLSELFGAV-HKVV 60

Query: 62  DVTLLNELAVSSLTRDSIPVP--------DYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           ++  + + + S+LT+    VP        + E   + +   +VP RN++  ++++ +A  
Sbjct: 61  ELPFVKDFSSSALTKKEEEVPTIKNSELDNLEKATETMEKVWVPARNMILFSISSGFAEF 120

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           V A+ + TG+   + + +PD  +EF+   N  +  G    ++ ++PL    K E   +  
Sbjct: 121 VDAKYIYTGLNVEEGATFPDNTEEFLNRFNSVLEYGTLNKVKMKSPLYKYSKKEIVKIGK 180

Query: 174 YY-GKLDLVR-NETLTCYNGFKGD--GCGHCAACNLRANGLNHYLADKPTVM 221
               KL++     + +CY+  K D   CG C +C  R         + PT  
Sbjct: 181 KLEEKLNVEFLKYSYSCYHDNKTDFLHCGTCESCMRRKRAFKEAGVEDPTEY 232


>UniRef50_D1PYH8 Transcription regulator ExsB n=5 Tax=Prevotella RepID=D1PYH8_9BACT
          Length = 242

 Score =  248 bits (634), Expect = 1e-64,   Method: Composition-based stats.
 Identities = 63/221 (28%), Positives = 97/221 (43%), Gaps = 8/221 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKV 60
           + A+++ SGG DS T L +   +      ++FDYG  H   EI  A     +LG   H +
Sbjct: 26  RDAIIIVSGGMDSITMLYEFQDR--IALGLSFDYGSNHNAREIPYAEMHCKRLGI-PHII 82

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  ++    SSL   +  +P+ +    G+ +T VP RN + L++AA  A     + ++
Sbjct: 83  IPLDFMSRYFKSSLLEGADAIPEGDYNEVGMKSTVVPFRNGIMLSIAAGMAESQNLKYIM 142

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D   YPDCR EF++A++ A   G    I+   P   I K +    A     L L
Sbjct: 143 MANHSGDHIIYPDCRPEFIRAMSKATQTGTFPGIKILAPYTDITKGDI---ACRGKALGL 199

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              ET +CY G +   CG C  C  R   L        T  
Sbjct: 200 DYTETWSCYKGGE-RHCGKCGTCLERIAALKDAGIKDLTEY 239


>UniRef50_A0B6A6 7-cyano-7-deazaguanine synthase n=4 Tax=Archaea RepID=QUEC_METTP
          Length = 224

 Score =  248 bits (633), Expect = 1e-64,   Method: Composition-based stats.
 Identities = 63/229 (27%), Positives = 99/229 (43%), Gaps = 13/229 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           M RAV + SGG DST     A +   +V+ +   YGQ+  R EI+    +A  +GA    
Sbjct: 1   MGRAVCLCSGGLDSTVAATIARRSGMDVYLIHVSYGQQAERREIEAIERIADAIGASDLM 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
              + L   +  S+LT     +P  E    +++  P T+V  RN + L++AA YA  + A
Sbjct: 61  CSRIDLFRNI--SALTTQGARIPRGEEVSLDSESTPPTWVYCRNTVLLSMAAAYAEYLGA 118

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMA---KDIRFETPLMWIDKAETWALAD 173
            ++  G    +   YPD R EFV+  N  +   +A   +  +   PL+ + K +   L  
Sbjct: 119 HSIYVGFNAEEAMSYPDNRPEFVEQFNALLEKAVASFSRPPKVVAPLVDMRKKDIVRLG- 177

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
               +      T +CY   +   CG C +C  R  G        PT   
Sbjct: 178 --ADIKAPLELTWSCYLNGEIH-CGTCESCQHRRRGFVEAGIPDPTEYQ 223


>UniRef50_Q12WI3 7-cyano-7-deazaguanine synthase n=6 Tax=cellular organisms
           RepID=QUEC_METBU
          Length = 233

 Score =  247 bits (630), Expect = 3e-64,   Method: Composition-based stats.
 Identities = 62/231 (26%), Positives = 104/231 (45%), Gaps = 16/231 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           R++++ S G DS   L  AL   D    +TFDYGQR  + EI+ +R +    G   H+++
Sbjct: 2   RSILLLSSGLDSVAALAIALDNSDVFMALTFDYGQRSVKKEIEYSRMVCEHYGIE-HRII 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPE----------ADGIPNTFVPGRNILFLTLAAIYA 111
            ++ L+ +  +SL    + VP+               D   + +VP RN +F+ +AA +A
Sbjct: 61  TLSWLSSITNTSLVNKDLDVPELSINDLIDGPDNITEDSAKSVWVPNRNGVFINIAASFA 120

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
                E VI G    +   +PD   EFV A++   S   A  ++   PL+ +DK      
Sbjct: 121 ESHGCEYVIAGFNGEEAKTFPDNSKEFVNAVDECFSYSTANGVKVLAPLIDMDKVSIVRK 180

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           A    +L      + +CY+G + + CG C +C  R         + P +  
Sbjct: 181 A---MELGAPLEYSWSCYHGAE-EPCGVCESCMRRKRAFLASGFEDPHIKK 227


>UniRef50_D1R557 Putative uncharacterized protein n=1 Tax=Parachlamydia
           acanthamoebae str. Hall's coccus RepID=D1R557_9CHLA
          Length = 221

 Score =  244 bits (623), Expect = 2e-63,   Method: Composition-based stats.
 Identities = 68/217 (31%), Positives = 99/217 (45%), Gaps = 13/217 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +A+V+ SGG DS   L +  +Q  E   ++FDYGQRHR E++ A+++A   G   H+V+ 
Sbjct: 2   KAIVLLSGGLDSAVMLARLHEQQIETLAISFDYGQRHRVELEFAKKIADFYGIE-HEVIP 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEA---DGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +  L     S+LT  + P      E      IP T+VP RN LFL  A   A    A+ +
Sbjct: 61  IDPLIFRGGSALTSSATPQKGKSKEDILKQSIPFTYVPARNTLFLAYALAKAEMHGADQI 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR-----FETPLMWIDKAETWALADY 174
             G    DF  YPDCR  + +A     +    + +        TPL+   K E       
Sbjct: 121 HFGSNAMDFLSYPDCRPGYFEAAQELFNRATKQSVEGKAPQIVTPLLKWTKEEIVKEGY- 179

Query: 175 YGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGL 210
             +L +    T +CY+    G  C  C AC LR +  
Sbjct: 180 --RLKVPFELTSSCYDPTANGQPCQQCDACTLRQDAF 214


>UniRef50_B4E7W5 7-cyano-7-deazaguanine synthase n=73 Tax=Bacteria RepID=QUEC_BURCJ
          Length = 244

 Score =  243 bits (620), Expect = 4e-63,   Method: Composition-based stats.
 Identities = 103/229 (44%), Positives = 145/229 (63%), Gaps = 22/229 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGAR------- 56
           A+V+FSGGQDS TC+  AL++Y  V  + FDYGQRHR E++    +   L  R       
Sbjct: 10  ALVLFSGGQDSATCVAWALERYQTVETLGFDYGQRHRVELECREGVREALKHRFPAWSDR 69

Query: 57  --AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
                ++D+++L  ++ +++TR      + E  A+G+PNTFVPGRN++F+T+AA  AY+ 
Sbjct: 70  LGDDHMIDLSVLGAISDTAMTRT----IEIETTANGLPNTFVPGRNLMFMTIAAAIAYRR 125

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADY 174
               ++ G+CETDFSGYPDCRD+ +KAL  A++LGM   +  ETPLMW+DKA+TW LA+ 
Sbjct: 126 GLRVLVGGMCETDFSGYPDCRDDTMKALQVALNLGMDTRMVLETPLMWLDKAQTWQLAEQ 185

Query: 175 YGK---LDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHYL 214
            G    ++L+R ET TCY G +      G GCG C AC LR  G   YL
Sbjct: 186 LGGDALVELIRVETHTCYVGERAELHDWGFGCGECPACKLRKRGYEAYL 234


>UniRef50_B0TFM0 Exsb protein, putative n=1 Tax=Heliobacterium modesticaldum Ice1
           RepID=B0TFM0_HELMI
          Length = 247

 Score =  242 bits (618), Expect = 8e-63,   Method: Composition-based stats.
 Identities = 85/248 (34%), Positives = 118/248 (47%), Gaps = 33/248 (13%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +RAVV+ SGG DSTTC+  A  + +++  +TF Y QRH  EI+ A+ +A   GA  H+++
Sbjct: 6   ERAVVILSGGLDSTTCMALADCRGEDIFALTFAYNQRHIREIEAAQAVACHYGA-HHQII 64

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADG---------------------IPNTFVPGRN 100
           D+  +   A S+LT   I      P                         IP T+VP RN
Sbjct: 65  DLGAV--FAASALTSTEIRPEVAAPLGGSAVAKMEAMGCAEKNPVAIATAIPATYVPARN 122

Query: 101 ILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIR 155
           ILFL  A  +A +V A  + TGV   D  GYPDCR EF+ A    + +G       + + 
Sbjct: 123 ILFLAYALSFAEKVGARRIYTGVNALDNPGYPDCRPEFIAAFQRMIDVGTKAGTEGRSVE 182

Query: 156 FETPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
             TPL+ ++KA+   LA     LD   + TLTCY G +   CG C +C LR  G      
Sbjct: 183 VFTPLIEMNKADIIRLALD---LDAPLHLTLTCYYG-ERPACGECNSCRLRLEGFRLLGK 238

Query: 216 DKPTVMAA 223
             P     
Sbjct: 239 GDPIAYRK 246


>UniRef50_UPI0001AF28A8 succinoglycan biosynthesis regulator n=2 Tax=Streptomyces
           filamentosus RepID=UPI0001AF28A8
          Length = 223

 Score =  240 bits (614), Expect = 2e-62,   Method: Composition-based stats.
 Identities = 75/226 (33%), Positives = 107/226 (47%), Gaps = 11/226 (4%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M R + + SGG DS         +  EVH ++ DYGQRHR E D AR  A ++GA  H++
Sbjct: 1   MARVIAIVSGGLDSVVLAHHLHAEGHEVHLLSIDYGQRHRKEHDYARIAADRIGAE-HEI 59

Query: 61  LDVTLL-NELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +D++ + + L   SLT   + VPD    A G  N  VP RN++ L++A   A  VKA+ V
Sbjct: 60  VDLSNVASMLPGYSLTDSQVDVPDQHYTAPGSVN-VVPNRNVILLSVAYGKAAAVKADQV 118

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAK----DIRFETPLMWIDKAETWALADYY 175
             G    D S  PDC  EF+ + N    +  A      +    P + + K +   L +  
Sbjct: 119 AIGALAGDASTAPDCSAEFISSFNAMEKIACAGYAPAHLEVVAPFIDLHKHDVIRLGE-- 176

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            KL +   ET +C+ G +   CG CAAC  R            TV 
Sbjct: 177 -KLGVDWTETWSCFKGTEVH-CGTCAACVDRIQAFERAETTDTTVY 220


>UniRef50_C6XGK1 ExsB protein n=9 Tax=Bacteria RepID=C6XGK1_LIBAP
          Length = 240

 Score =  239 bits (610), Expect = 7e-62,   Method: Composition-based stats.
 Identities = 90/231 (38%), Positives = 136/231 (58%), Gaps = 26/231 (11%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAH----- 58
           A+++FSGGQDS+TCL  AL ++D V  ++FDYGQR++ E++    L ++           
Sbjct: 11  ALLLFSGGQDSSTCLSWALDRFDRVETLSFDYGQRNKVELEC--RLCVRKKIVELMPKWK 68

Query: 59  ------KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
                  +L + +L +++ SSLT++       + + + +PNTFVPGRNI+FL  AA  AY
Sbjct: 69  DSLGEDHILPLAILGDISHSSLTKN----VAMKIQDNNLPNTFVPGRNIIFLVFAATLAY 124

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
           ++    ++ GVCETD+SGYPDCR + ++A+  A++LGM   +   TPLMW+ K ETW LA
Sbjct: 125 RLGITNIVIGVCETDYSGYPDCRHDTIRAIETAINLGMESHVTVHTPLMWLKKYETWKLA 184

Query: 173 DYYGK---LDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHYL 214
              G    ++L+  E+ TCY G +      G GC  C AC LR  G   + 
Sbjct: 185 QDIGGQDLVNLILEESHTCYLGKRDKRYEWGYGCNSCPACYLRQKGWMEFK 235


>UniRef50_UPI0001C418E0 queuosine biosynthesis protein QueC n=1 Tax=Methanobrevibacter
           ruminantium M1 RepID=UPI0001C418E0
          Length = 211

 Score =  238 bits (609), Expect = 9e-62,   Method: Composition-based stats.
 Identities = 65/215 (30%), Positives = 97/215 (45%), Gaps = 10/215 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K  V++ SGG DSTT L + L + + V  ++F+YGQ+   EI+ A E+  K+    H V 
Sbjct: 3   KDVVLILSGGLDSTTLLYKLLNEGNNVTALSFNYGQKAAIEIERASEIC-KMNDVNHFVF 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+  + +L  SSLT                 +T VP RN + L LA  +A     E V  
Sbjct: 62  DIRNIVDLLSSSLTDKD------NDNVQEPKSTVVPSRNTILLELATAFAISNNLEEVYY 115

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G  + D   YPD   EF+K +N    +   + I    P +  DK +   LA    +L + 
Sbjct: 116 GAIKADTGDYPDTTPEFLKQINELNKVNNYEYIPVCAPFIDTDKKDVVKLA---LELGVP 172

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
             +T +CY    G  CG C +C  R   +     +
Sbjct: 173 IEKTWSCYVNNDGTPCGECFSCKSRIEAIKEAKEE 207


>UniRef50_Q857D1 Gp3 n=2 Tax=unclassified Siphoviridae RepID=Q857D1_9CAUD
          Length = 235

 Score =  237 bits (604), Expect = 3e-61,   Method: Composition-based stats.
 Identities = 73/222 (32%), Positives = 101/222 (45%), Gaps = 10/222 (4%)

Query: 5   VVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +V+ SGG DS+T L  AL +   D+   +  DYGQRH  E   ++ +A   GA   + LD
Sbjct: 8   LVLLSGGMDSSTLLAWALDRPDVDQASALFVDYGQRHVVERASSQLVAQHYGAAWSE-LD 66

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +        S+LT  +  VP      D +  T VP RN   ++ AA  A  +  +AV+T 
Sbjct: 67  LRAFGASVNSALTVMAREVPHGHYAEDNMVQTVVPNRNATLISAAAGIAASLGHDAVLTA 126

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           V   D + YPDCR EFV A++ A   G    +    P    DKA+   L    G+L +  
Sbjct: 127 VHVGDHAVYPDCRPEFVAAIDMATFAGC--GVHVVAPFQTGDKADIARLG---GQLGVPY 181

Query: 183 NETLTCYNGF--KGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           + T +CY G       CG C  C  RA          PT  A
Sbjct: 182 HLTWSCYEGGLNDARHCGRCGTCVERAEAFAEAGVPDPTEYA 223


>UniRef50_Q0BQ87 7-cyano-7-deazaguanine synthase n=76 Tax=Bacteria RepID=QUEC_GRABC
          Length = 252

 Score =  236 bits (603), Expect = 5e-61,   Method: Composition-based stats.
 Identities = 99/234 (42%), Positives = 136/234 (58%), Gaps = 27/234 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV---ARELALKLGARA-- 57
            A+V+FSGGQDSTTCL  AL+++  V  + F YGQRH  E++     R++   +  R+  
Sbjct: 17  HALVLFSGGQDSTTCLAWALERFAHVETIGFTYGQRHSVEMECRGRVRQVLAHMSGRSGA 76

Query: 58  --------HKVLDV-TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
                     VLD+   L+ +  S+LT  S      E    G+P +FVPGRN++FLT AA
Sbjct: 77  DWGSRLGDDHVLDLGNALHHVGQSALTGRS----PIEIGQGGLPTSFVPGRNLIFLTYAA 132

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAET 168
              ++     ++ G+CETD+SGYPDCRD+ +KA+  A++ GM      ETPLMW+ KAET
Sbjct: 133 ALGWRRGLRRMVGGMCETDYSGYPDCRDDTIKAMQLALNTGMEAHFVLETPLMWLTKAET 192

Query: 169 WALADYYGK---LDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
           WALAD  G    + L+  ET TCY G +      G GCG C AC+LRA G   +
Sbjct: 193 WALADSLGGPALVALIEEETHTCYLGDRSQRHPWGYGCGECPACSLRAEGWREW 246


>UniRef50_Q5JHU5 7-cyano-7-deazaguanine synthase n=6 Tax=Thermococcus
           RepID=QUEC_PYRKO
          Length = 239

 Score =  236 bits (602), Expect = 6e-61,   Method: Composition-based stats.
 Identities = 65/237 (27%), Positives = 104/237 (43%), Gaps = 18/237 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MKRAVV+FSGG DST CL  A + YDEV  +T +YG       +   E   K      K+
Sbjct: 1   MKRAVVLFSGGLDSTACLYWAKKNYDEVIMLTVNYGSNEEKVTNKVAEFFSKELDVPLKI 60

Query: 61  LDVTLLNELA---VSSLTRDSIP------VPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           + +  L E +    ++L     P      + + E   +   + +VP RN++ + +AA   
Sbjct: 61  VRLDFLEEFSKLRGTTLVGGETPKVTGEELENIEVAQETAKSVWVPARNVVLIAVAASLL 120

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             +    ++ G    + + +PD   EFV+ +N  +  G   ++R   PL+ +DK     L
Sbjct: 121 DALGGGDIVVGFNAEEGATFPDNTPEFVEKMNEMLKYGTMAEVRVVAPLINLDKRGIAKL 180

Query: 172 ADYYGKLDLVRNETLTCYNG------FKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                +L      + +CY         K   CG C +C  R  GL   + +  TV A
Sbjct: 181 ---LKELGAKYEYSNSCYMPKGFTEDGKPIHCGECESCVRRHRGLIEGIGEDKTVYA 234


>UniRef50_Q8U4N3 7-cyano-7-deazaguanine synthase n=3 Tax=Pyrococcus RepID=QUEC_PYRFU
          Length = 240

 Score =  232 bits (593), Expect = 6e-60,   Method: Composition-based stats.
 Identities = 72/239 (30%), Positives = 113/239 (47%), Gaps = 21/239 (8%)

Query: 1   MKR-AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAH 58
           MKR AVV+FSGG DST CL  A +QYDEV  +T +YG    R    VA   + +L  R  
Sbjct: 1   MKRRAVVLFSGGLDSTACLYWAKKQYDEVIMLTVNYGSNEERVTNKVAEYFSKELDVR-L 59

Query: 59  KVLDVTLLNELA---VSSLTRDSIP------VPDYEPEADGIPNTFVPGRNILFLTLAAI 109
           K++ +  L E +    SSL    +P      + D E  ++   + ++P RN++ +++AA 
Sbjct: 60  KIVKLDFLKEFSEIRGSSLVGGEVPRVTAEELEDIEKASETARSVWIPARNLVLISVAAS 119

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
               +    +I G    + + +PD   EFV+ LN A+       ++   PL+ +DK    
Sbjct: 120 LLDALGGGDIIVGFNAEEATTFPDNSREFVEKLNEALRFATLNPVKVVAPLIDLDKRGIA 179

Query: 170 ALADYYGKLDLVRNETLTCYNG------FKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
            L     +L+     + +CYN        +   CG C +C  R  GL   + +  TV A
Sbjct: 180 KL---LKELNAKYEYSNSCYNPKGFTEDGRPIHCGECESCVRRHKGLIEGIGEDKTVYA 235


>UniRef50_D2RLP8 ExsB protein n=3 Tax=Bacteria RepID=D2RLP8_ACIFE
          Length = 232

 Score =  231 bits (589), Expect = 2e-59,   Method: Composition-based stats.
 Identities = 69/233 (29%), Positives = 106/233 (45%), Gaps = 19/233 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+A+V+ SGG DSTTC+  A++++  + V  V+  YGQ+HR E++ AR++A   G   H 
Sbjct: 3   KKALVLSSGGVDSTTCVSVAVEEFGKENVSTVSIFYGQKHRKELECARKVAEYYGL-PHY 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYE-------PEADGIPNTFVPGRNILFLTLAAIYA- 111
            LD++ + + +  SL   S     +E          +G  +T+VP RN L L+  A  A 
Sbjct: 62  ELDLSKIFQYSNCSLLDHSTEEIVHESYEQQIAENGEGKVSTYVPFRNGLMLSAVASLAM 121

Query: 112 --YQVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
             Y+    ++  G    D +G  Y DC + F+ A+  A+SLG         P     KA 
Sbjct: 122 SIYEKDDVSIFIGAHADDAAGNAYADCSEAFIGAMGKAISLGTYNQCHIVAPFASCSKAG 181

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
                    +L    + T +CY G     CG C  C  RA          P +
Sbjct: 182 VVKRG---LELHTPYDLTWSCYEGG-DKPCGTCGTCIDRARAFAANGVKDPAL 230


>UniRef50_Q8UAM7 7-cyano-7-deazaguanine synthase n=71 Tax=Alphaproteobacteria
           RepID=QUEC_AGRT5
          Length = 236

 Score =  231 bits (589), Expect = 2e-59,   Method: Composition-based stats.
 Identities = 70/221 (31%), Positives = 103/221 (46%), Gaps = 7/221 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + +V+ SGG DS +   +   +++ +  V+FDYGQRH+ E+D A + A +LG   H ++D
Sbjct: 2   KTIVICSGGLDSVSLAHKIAAEHELLALVSFDYGQRHKKELDFAADCAKRLGV-PHHLID 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +  +      S   D I VPD     + + +T VP RN + LT+A   A   +A+AV   
Sbjct: 61  IGTIGAHLTGSALTDDIDVPDGHYAEETMRSTVVPNRNAIMLTIAFGLAAAQQADAVAIA 120

Query: 123 VCETDFSGYPDCRDEFVKALN--HAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           V   D   YPDCR  F+ + N   A +L    D+    P + + KA     A  YG    
Sbjct: 121 VHGGDHFIYPDCRPGFIGSFNAMQAHALEGYADVTLFAPYVTVSKAAIVTDAVKYGT--- 177

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              ET +CY G     CG C  C  R    +      PT  
Sbjct: 178 PFGETWSCYKGGL-RHCGRCGTCVERREAFHLAGVTDPTEY 217


>UniRef50_C0WAE7 Archaeosine biosynthesis protein queC n=3 Tax=Bacteria
           RepID=C0WAE7_9FIRM
          Length = 233

 Score =  231 bits (589), Expect = 2e-59,   Method: Composition-based stats.
 Identities = 67/233 (28%), Positives = 104/233 (44%), Gaps = 19/233 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+A+V+ SGG DSTTC+  A+ +   + V  V+  YGQ+HR E+D A+++A       H 
Sbjct: 3   KKALVLSSGGVDSTTCVSVAVHELGRENVATVSIFYGQKHRKELDAAQKVADFYHV-PHY 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIP-------NTFVPGRNILFLTLAAIYA- 111
            LD++ + + +  SL   S     +E   D I        +T+VP RN L L+  A  A 
Sbjct: 62  ELDLSKIFQYSNCSLLSHSTEEIIHESYEDQIAQNGEGKVSTYVPFRNGLMLSAVASLAM 121

Query: 112 --YQVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
             ++     +  G    D +G  Y DC + F  A+  A+S+G  +      P + ++KA 
Sbjct: 122 SLFEKDDVDIYLGAHADDAAGNAYADCSEAFTDAMGKAISIGTYEQCHLVAPFVEVNKAG 181

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
                    +L      T +CY G     CG C  C  RA          P +
Sbjct: 182 IVKKG---LELGTPYELTWSCYEGG-DRPCGTCGTCIDRARAFAANGVPDPAL 230


>UniRef50_Q6WI28 ExsB protein n=1 Tax=Vibrio phage KVP40 RepID=Q6WI28_BPKV4
          Length = 238

 Score =  228 bits (583), Expect = 9e-59,   Method: Composition-based stats.
 Identities = 64/227 (28%), Positives = 108/227 (47%), Gaps = 11/227 (4%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + V V SGG DST    + + ++  D V  ++F YGQ+   E++ A+    KLG   H++
Sbjct: 2   KVVSVLSGGLDSTILTYKLVDEFGKDNVFALSFYYGQKQSIELEKAKLTCQKLGIE-HRL 60

Query: 61  LDVTLLNELAV---SSLTRDSIPVPDYEPE-ADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
           +D++ L E+     +++    + +P  +    D  P T VP RN+L  T+A  +A     
Sbjct: 61  IDISFLGEVVAPVCANIAGSDVAMPTIQDVLGDPQPPTEVPYRNMLLNTIAFSFAQANGC 120

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            AV TG+   D  GY DC  +F+  +N   +L    +I    P   + KA+  A+     
Sbjct: 121 SAVFTGIQVHDAYGYWDCTQDFLDGMNKISALNRQHEITLHAPFAQMSKADEIAIGV--- 177

Query: 177 KLDLVRNETLTCYNGFK-GDGCGHCAACNLRANGLNHYLADKPTVMA 222
           +L +   ++LTCY+  + G+ CG C +C  R           P   +
Sbjct: 178 ELGVPFEDSLTCYDPNEAGESCGKCPSCAERIMNFGKAGIVDPAPYS 224


>UniRef50_C7ML00 ExsB protein n=9 Tax=Bacteria RepID=C7ML00_CRYCD
          Length = 240

 Score =  228 bits (583), Expect = 1e-58,   Method: Composition-based stats.
 Identities = 77/231 (33%), Positives = 109/231 (47%), Gaps = 17/231 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
            A+V+ SGG DSTTCL  A+ ++    V  V+F YGQRH  E+D A  +A   G   H V
Sbjct: 14  HALVLSSGGVDSTTCLALAVDRFGQANVSTVSFFYGQRHSRELDAAAAVAEHYGV-HHYV 72

Query: 61  LDVTLLNELAVSSLTRDSIPVPDY-----EPEADGIPNTFVPGRNILFLTLAAIYA---Y 112
           LD+  +   + ++L   S     +     + E DG PNT+VP RN L L+ AA  A   +
Sbjct: 73  LDLASVMHYSNNALMATSTQHVPHGSYAQQMEDDGHPNTYVPFRNGLMLSAAAALAASLF 132

Query: 113 QVKAEAVITGVCETDFSG--YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
              + A+  G    D +G  YPDC   F   +  A+S G   ++R ETP +  +KA+  A
Sbjct: 133 PDTSCALYLGAHADDAAGNAYPDCSPAFTAHMGRAISFGTYGNVRLETPFVNQNKAQVVA 192

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                 +L +    T +CY G +   CG CA C  R           P   
Sbjct: 193 EG---LRLGVPYEMTWSCYEGGE-KPCGTCATCLDRIAAFEANGVKDPVSY 239


>UniRef50_A9A531 ExsB family protein n=2 Tax=Thaumarchaeota RepID=A9A531_NITMS
          Length = 224

 Score =  226 bits (576), Expect = 5e-58,   Method: Composition-based stats.
 Identities = 75/232 (32%), Positives = 106/232 (45%), Gaps = 21/232 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AVVVFSGG DS  C V  L+   E++ +TF YGQ+   EI   +  A KLG + HK+
Sbjct: 1   MKKAVVVFSGGVDS-VCAVSFLKSKYELYGITFSYGQKANMEISATKSFAKKLGLKQHKI 59

Query: 61  LDVTLLNELAVSS--LTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +D+  + EL   S  LT     +P          +  VP RN +FL++A+ +AY + A  
Sbjct: 60  IDIGFMKELYGDSNVLTSSKRKIPSKFE-----YSIVVPIRNAVFLSVASAWAYTLNASL 114

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLG--------MAKDIRFETPLMW-IDKAETW 169
           V+ G    D   YPDCR  F K L  A + G        + K I+  +P    + K++  
Sbjct: 115 VVYGAHTGD-KYYPDCRPAFAKKLESAFNQGEIDGINSKIRKKIKIWSPYREGLSKSDLL 173

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                   L      T +CY+  K   CG C +CN R            T  
Sbjct: 174 KKGKKV--LGDSIFRTWSCYS-NKKLHCGVCESCNNRKIAFEKAGVTDKTKY 222


>UniRef50_A8AAU7 ExsB protein n=1 Tax=Ignicoccus hospitalis KIN4/I
           RepID=A8AAU7_IGNH4
          Length = 260

 Score =  225 bits (573), Expect = 1e-57,   Method: Composition-based stats.
 Identities = 67/246 (27%), Positives = 97/246 (39%), Gaps = 30/246 (12%)

Query: 5   VVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           V + SGG DS T    A+ ++  D    +T  YGQRH  E+  A E+A   GA  H V+D
Sbjct: 4   VGLVSGGMDSATAFADAINKFNPDVKVALTVVYGQRHARELKAAAEVAKFYGA-QHLVVD 62

Query: 63  VTLL--NELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV------ 114
           ++ L      VS+L          E     +P T+VP RN + L  A     ++      
Sbjct: 63  LSDLFEKVKEVSALLGGRGVPEAEELVPGLVPPTYVPQRNAVLLASAGAILEKLMIERND 122

Query: 115 KAEAVITGVCETDFSG----YPDCRDEFVKALNHAVSLGM-----------AKDIRFETP 159
               +  G   TD++     YPD R EFV+A+  A++ G               I    P
Sbjct: 123 GLGVISVGFHGTDYAPGEPVYPDTRPEFVEAMERALNEGSSVVFNFKRAGREAKITIYAP 182

Query: 160 LMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
            + + K +    A    +L +    T TCY G     CG C AC  R           P 
Sbjct: 183 FVRLKKKDVVKRAL---ELGVPLELTWTCYRGG-DRPCGTCPACVSRLRAFMEAGVPDPL 238

Query: 220 VMAAMK 225
             +  +
Sbjct: 239 TESYER 244


>UniRef50_Q0W7U0 Succinoglycan biosynthesis regulator n=1 Tax=uncultured
           methanogenic archaeon RC-I RepID=Q0W7U0_UNCMA
          Length = 222

 Score =  225 bits (573), Expect = 1e-57,   Method: Composition-based stats.
 Identities = 54/224 (24%), Positives = 100/224 (44%), Gaps = 15/224 (6%)

Query: 7   VFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVLDVTL 65
           + S G DS     +   ++     +TFDYGQR    EI++AR++  +   R H+V+++  
Sbjct: 1   MLSSGLDSVVAFKKEHDEHGVALAITFDYGQRAASKEIEMARQICARYHVR-HEVIELPW 59

Query: 66  LNELAVSSLTRDSIPVP-DYEPEADGIPN--------TFVPGRNILFLTLAAIYAYQVKA 116
           L ++  ++L      VP   E E D I           +VP RN  F+ +AA +      
Sbjct: 60  LKQITRTALVSRQAEVPEPTEAELDEIKGRALETAHLVWVPNRNGAFINIAAAFGDAYGY 119

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
             ++ G    + + +PD    +V A+N +++L   K ++   P++  DK E         
Sbjct: 120 NRIVCGFNAEEGATFPDNTPAYVDAVNKSLALSCEKQVQVVAPVIHFDKEEIIREGV--- 176

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
           +++   + + +CY   +   CG C +C  RA          P++
Sbjct: 177 RIEAPLDLSWSCYFAGE-KPCGKCESCMRRARAFKRAGVPDPSL 219


>UniRef50_D1R584 Putative uncharacterized protein n=1 Tax=Parachlamydia
           acanthamoebae str. Hall's coccus RepID=D1R584_9CHLA
          Length = 231

 Score =  222 bits (565), Expect = 1e-56,   Method: Composition-based stats.
 Identities = 77/227 (33%), Positives = 119/227 (52%), Gaps = 9/227 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+AVV+ SGG DS+ CL  A++ +  + V  ++F Y QRH +E++ A+++        H 
Sbjct: 5   KKAVVIHSGGMDSSICLALAIRTFSVENVLSLSFIYDQRHSSELEQAKKICQSWNV-DHI 63

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +L +  L E+  ++LT  S+P+     EA   PNT V GRN L   L AI+A+++ A  +
Sbjct: 64  ILSIPCLKEITTNALTDTSLPIQCGNGEA---PNTLVVGRNGLMAQLGAIHAHELGAHCI 120

Query: 120 ITGV--CETDFSGYPDCRDEFVK-ALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
             G+   E + SGY DC   ++                  ETPL+ + K +T  LA   G
Sbjct: 121 YMGIIGVEGNHSGYRDCSRAYMDLKQTLLRLDLDDHLFTIETPLVDMSKKKTLELAQEMG 180

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
            L+ + NET+TCY G +  GC  C AC LR  G+  + A+ P+    
Sbjct: 181 VLEFLLNETITCYEGLEKLGCQKCPACILRNEGIRQFSAENPSFKLP 227


>UniRef50_Q2IUE0 7-cyano-7-deazaguanine synthase 2 n=1 Tax=Rhodopseudomonas
           palustris HaA2 RepID=QUEC2_RHOP2
          Length = 210

 Score =  221 bits (563), Expect = 2e-56,   Method: Composition-based stats.
 Identities = 71/215 (33%), Positives = 100/215 (46%), Gaps = 14/215 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K+ +V+ SGG DSTTCL+QA  +  +V  +  DYGQR   E+  A     K     H V+
Sbjct: 4   KKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQRLLVEMMFAEGQCEKYSIPRH-VI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +V          L R        E  A  +   F+PGRNI+FL L   +A  + A+ +  
Sbjct: 63  NVNWQKAERQIPLNRS------VEEMAHSVSPAFLPGRNIVFLGLGHAHAAGIGADELQI 116

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+   DFSGYPDC  +F  +    +++G     +   PL+ + K E   LA     L L 
Sbjct: 117 GLNCVDFSGYPDCTTQFYDSYCTMLNIGNPGGPKLVAPLLKMSKPEIARLAST---LGLQ 173

Query: 182 RNETLTCYNGFKGDG----CGHCAACNLRANGLNH 212
           RN+T +CY     +G    CG C AC L       
Sbjct: 174 RNDTWSCYRPQIREGSIVACGECDACKLHEFAWQE 208


>UniRef50_B3G2Q8 Putative uncharacterized protein n=1 Tax=Pseudomonas aeruginosa
           RepID=B3G2Q8_PSEAE
          Length = 222

 Score =  219 bits (558), Expect = 7e-56,   Method: Composition-based stats.
 Identities = 69/214 (32%), Positives = 100/214 (46%), Gaps = 14/214 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           ++ VV+ SGG DST CL+ A  +  EV  +   Y Q H  E+D A     + G    K++
Sbjct: 16  RKIVVLHSGGLDSTVCLLLAHSRGHEVVSLGVSYNQHHHVELDYAAAQCARYGIE-RKIV 74

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            V       V    R    +        G+ + F+PGRN +FL LA+  A  + A+ V T
Sbjct: 75  SVAWSKPARVIPKNRSMAEI------KKGVSSAFLPGRNGIFLMLASAEAAGIGADEVWT 128

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+   DFSGYPDCR EF+++    +S  + K  +   PL    K +   LA    +L L 
Sbjct: 129 GINSVDFSGYPDCRPEFIESFRKMLSYAIPKGPKLSAPLQKKSKPQIAKLAH---RLGLQ 185

Query: 182 RNETLTCYNGFKGD----GCGHCAACNLRANGLN 211
           R++T +CY     +     CG C AC L      
Sbjct: 186 RHDTWSCYRPKIAEAGFVPCGACDACKLHEFAWQ 219


>UniRef50_UPI00016988F1 exsB protein n=1 Tax=Endoriftia persephone 'Hot96_1+Hot96_2'
           RepID=UPI00016988F1
          Length = 213

 Score =  218 bits (555), Expect = 2e-55,   Method: Composition-based stats.
 Identities = 71/163 (43%), Positives = 100/163 (61%), Gaps = 8/163 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           KRAVV+ SGG DS T L  A QQ  E H ++  YGQRH AE+D AR++A  LGA  HK++
Sbjct: 5   KRAVVLLSGGLDSATVLAMARQQGFECHALSMLYGQRHAAELDAARQVAQALGAVDHKLI 64

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++  L+++  S+LT + I VP     ++GIP T+VP RN +FL+LA  +A  ++A+ +  
Sbjct: 65  NIG-LDDIGGSALTDEQIAVPKTP--SEGIPPTYVPARNTVFLSLALGWAEVLEAQDIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETP 159
           GV   D+SGYPDCR EF+ A     +L        + I   TP
Sbjct: 122 GVNAVDYSGYPDCRPEFIVAFEQLANLATKAGVEGRRIHIHTP 164


>UniRef50_B3T674 Putative ExsB n=1 Tax=uncultured marine crenarchaeote
           HF4000_ANIW141O9 RepID=B3T674_9ARCH
          Length = 233

 Score =  217 bits (553), Expect = 3e-55,   Method: Composition-based stats.
 Identities = 73/232 (31%), Positives = 107/232 (46%), Gaps = 21/232 (9%)

Query: 1   MKRAVVVFSGGQDS-TTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           MK+AVVVFSGG DS +TC    L+Q  E++ ++F YGQ+   EI  A+  A  LG + HK
Sbjct: 10  MKKAVVVFSGGIDSVSTCTY--LKQKYELYGISFLYGQKANQEIKKAKSFAKILGLKEHK 67

Query: 60  VLDVTLLNELAV--SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           V+D+  + +L    ++LT     +P          +  VP RN +FL++A  +A+ + A 
Sbjct: 68  VVDINFMKKLYGDTNALTSSKKKIPS-----KFNYSVVVPIRNGIFLSIATAWAFSINAS 122

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSL--------GMAKDIRFETPLMWIDKAETW 169
            V  G    D   YPDCR  F K +  A +         G+ K I+  +P      ++  
Sbjct: 123 LVAYGAHMGD-KNYPDCRPAFSKKIEFAFNQGEIDGIKSGIRKRIQIWSPYKAKL-SKKQ 180

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            L   Y  L      T +CY   K   CG C +CN R         +  T  
Sbjct: 181 LLKKGYDTLGDKIFNTWSCYKNGKVQ-CGKCESCNNRKAVFLEAGIEDKTPY 231


>UniRef50_C3XFP7 Queuosine biosynthesis protein queC n=1 Tax=Helicobacter bilis ATCC
           43879 RepID=C3XFP7_9HELI
          Length = 213

 Score =  215 bits (547), Expect = 1e-54,   Method: Composition-based stats.
 Identities = 54/213 (25%), Positives = 94/213 (44%), Gaps = 8/213 (3%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVLDV 63
           +V+FSGG DSTT L  + ++    H + F Y   H + EID A ++       ++ +LD+
Sbjct: 3   LVLFSGGVDSTTALFWSKKECAT-HALIFKYPSLHNKREIDAAVQICKDENV-SYTLLDM 60

Query: 64  TLLNELAVSSLTRDSIP-VPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           + +     S+L ++S   +P      + +    VP RN +FL++AA  A  +  + ++  
Sbjct: 61  SSVFVGFRSALLQNSSESIPQDSYNLESMAKLVVPFRNGIFLSVAAGLAESLGYKEIVLA 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDC   F+ ++N ++  G    ++  +P    DK E   L      L +  
Sbjct: 121 NHAGDHPLYPDCTQSFIDSMNESIMQGSGGAVKLCSPFCNWDKKEIVKLGLS---LGVDY 177

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
            +T +CY G +   C  C  C            
Sbjct: 178 TKTYSCYRGGE-KHCNTCPTCIESNESFLANGI 209


>UniRef50_Q6MKZ5 7-cyano-7-deazaguanine synthase n=1 Tax=Bdellovibrio bacteriovorus
           RepID=QUEC_BDEBA
          Length = 231

 Score =  213 bits (543), Expect = 3e-54,   Method: Composition-based stats.
 Identities = 57/225 (25%), Positives = 99/225 (44%), Gaps = 15/225 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDE-VHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           K+ VV+ S G DST    +A++ + E V  +TF+YGQR  + E++ +  +A  LG   HK
Sbjct: 5   KKVVVLLSAGLDSTVNAYEAIKHHHEIVLALTFNYGQRAAKKELEASANIAKHLGI-PHK 63

Query: 60  VLDVTLLNELAVSSL--------TRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           V+++    +   SSL        T  ++ + + +   +   + +VP RN +FL +AA YA
Sbjct: 64  VVELPWFKDFNKSSLLVEDQAVPTGSAVEIDNQQKSEETAKSVWVPNRNGIFLNIAAAYA 123

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             + A+AVI G    + + +PD   EF++    ++    +  +        + K +   L
Sbjct: 124 EALGADAVIPGFNAEEAATFPDNSREFLEQATKSLWYSTSNHVTVGCYTAHLKKPDIVRL 183

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
                 L +       CY       CG C +C            D
Sbjct: 184 GQ---GLKVPWELIWPCYFSG-DKWCGQCESCLRSKRAFASANID 224


>UniRef50_Q981C9 7-cyano-7-deazaguanine synthase n=13 Tax=Thermoprotei
           RepID=QUEC_SULSO
          Length = 464

 Score =  212 bits (541), Expect = 6e-54,   Method: Composition-based stats.
 Identities = 53/224 (23%), Positives = 94/224 (41%), Gaps = 10/224 (4%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           R +VV SGG DST    + L++  EV  + F+Y  +    E +  R++A  L       +
Sbjct: 243 RVLVVASGGLDSTVAATKLLREGHEVTLIHFNYHHKAEEKEREAVRKIAEYLQI-PLLEI 301

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGI--PNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +  L   +  ++L +    +       +G    + +VP RN++F +++   A     +A+
Sbjct: 302 NTDLFKIIGHATLIKGGGEIVKDRKGEEGAEFAHEWVPARNLIFFSVSLAIAEAYGYDAI 361

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHA--VSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
            +G+   +   YPD   EF++ LN     + G  K I    P+  + K E   L     +
Sbjct: 362 ASGINLEEAGAYPDNEMEFIRMLNKLSPYATGPNKRIEILMPVGNLVKHEIVKLGY---E 418

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           +    + T +CY G +   CG C  C +R           P   
Sbjct: 419 IGAPLHLTWSCYEGGQ-KHCGKCGPCYMRKMAFRINGLKDPVEY 461


>UniRef50_Q8TWG9 7-cyano-7-deazaguanine synthase n=1 Tax=Methanopyrus kandleri
           RepID=QUEC_METKA
          Length = 229

 Score =  212 bits (540), Expect = 8e-54,   Method: Composition-based stats.
 Identities = 58/233 (24%), Positives = 91/233 (39%), Gaps = 18/233 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRA-EIDVARELALKLGARAH 58
           M   VV+ SGG DS+     A+++    V C+  DYGQR+   E   A  +A  +GA   
Sbjct: 1   MATTVVLCSGGLDSSVIAKWAVEELGGRVICLFVDYGQRNAPFERRAAERIAEAVGAEFE 60

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE- 117
            V    L      + +    +P    E   + +   ++P RN   L +AA     +  E 
Sbjct: 61  TVGTFWLRRLCPDNPMFAGRLP---REAGTEDLSANWLPARNWNLLGVAAALCDHLYLEG 117

Query: 118 -----AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 ++ G+   +   +PD   EF  A+  A+  G+    R  +PL  + K     L 
Sbjct: 118 EDDEFHIVWGINAEEAERFPDNTKEFADAVAEALKRGLPSRPRLHSPLAELYKPGIVRLG 177

Query: 173 DYYGKLDLVRNETLTCYNGFKGD----GCGHCAACNLRANGLNHYLADKPTVM 221
               +L      +++CYN    D     CG C AC  R         + PT  
Sbjct: 178 S---ELGAPMELSVSCYNPIWEDDTPVHCGECEACYHRKRAFERAGIEDPTEY 227


>UniRef50_A4J5C7 7-cyano-7-deazaguanine synthase n=4 Tax=Clostridia RepID=QUEC_DESRM
          Length = 228

 Score =  211 bits (537), Expect = 2e-53,   Method: Composition-based stats.
 Identities = 55/223 (24%), Positives = 96/223 (43%), Gaps = 12/223 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           +VV+ SGG DS   L  A  + +    +T +Y QR   +   A     +  A  H+V+++
Sbjct: 3   SVVLLSGGLDSAVNLAFARTEGNVKLALTINYRQRAAHKEIAAAAQLARYYAIPHRVIEL 62

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEA--------DGIPNTFVPGRNILFLTLAAIYAYQVK 115
             L E+  ++L R    +P+ + E               +VP RN LF+ +AA +A  + 
Sbjct: 63  PWLAEITKTALVRSESQLPEPKTEELDQLELAGKTAAEVWVPNRNGLFVNIAACFAETLH 122

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
            E V+ G    + + +PD   EF  A + A+    A  ++  +    ++K +  AL    
Sbjct: 123 CEIVVAGFNCEEAATFPDNTPEFALAASAAMQYSTANQVKVLSYTGRLNKKDIVALGQ-- 180

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
            +L L      +CY G +   CG C +C       +    + P
Sbjct: 181 -RLQLPWELIWSCYRG-EALMCGKCESCQRMIRAFHSLGLNLP 221


>UniRef50_B1XTB6 ExsB protein n=1 Tax=Polynucleobacter necessarius subsp.
           necessarius STIR1 RepID=B1XTB6_POLNS
          Length = 208

 Score =  198 bits (503), Expect = 2e-49,   Method: Composition-based stats.
 Identities = 53/183 (28%), Positives = 86/183 (46%), Gaps = 13/183 (7%)

Query: 12  QDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTLLNELAV 71
            DSTT L  A       + ++  YGQRH +E+  A+ +A ++G   H+V+++ L      
Sbjct: 22  IDSTTVLALAKDSDYTPYALSVGYGQRHSSELAAAKHIAKQIGVARHEVVNLDLTR-FGG 80

Query: 72  SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVCETDFSGY 131
           S+LT   I VP    +   IP T+VP RN + L+LA  +   +    +  G    D+SGY
Sbjct: 81  SALTDTKIAVPTSPGKDHEIPVTYVPARNTILLSLALGWTESLGGLDIFYGANSVDYSGY 140

Query: 132 PDCRDEFVKALNHAVSLGMAKDI---------RFETPLMWIDKAETWALADYYGKLDLVR 182
           PDCR E V +  H  +L     +         R   P++ + KA+   L      + +  
Sbjct: 141 PDCRPECVASFEHMANLATKAGVEAINNKNRFRVHAPIVSLTKAQIIQLGST---MGVDY 197

Query: 183 NET 185
           ++T
Sbjct: 198 SQT 200


>UniRef50_Q96ZD9 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Sulfolobus tokodaii
           RepID=QUEC1_SULTO
          Length = 202

 Score =  193 bits (490), Expect = 5e-48,   Method: Composition-based stats.
 Identities = 52/220 (23%), Positives = 91/220 (41%), Gaps = 24/220 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS++      ++  +  C+  +YGQR  R ++  ++ +  KL  +   V 
Sbjct: 2   KALLLMSGGLDSSSAAYYYTRRGLDFDCLFINYGQRSARMQLRSSKIICEKLN-KKLLVA 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+  + E     L    I +  +EP         +  RN++ + +A  +A +   E +I 
Sbjct: 61  DIRKIRE-----LFISDIWLKPHEP---------ITHRNLVIIPIAIAFAKEKGYEEIII 106

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              + D   Y   R E +K L    +LG    ++  TP   + K+    L    G   L 
Sbjct: 107 ASVKEDCE-YEQNRIEIIKELK---NLGEILKVKVSTPFAGMPKSFLLKLGVSAG---LD 159

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            + T +C  G K   CG C+ C  R           PT  
Sbjct: 160 PSLTYSCLLGHKYH-CGQCSQCLKRKEAFKSANIQDPTKY 198


>UniRef50_D2R400 ExsB family protein n=1 Tax=Pirellula staleyi DSM 6068
           RepID=D2R400_9PLAN
          Length = 236

 Score =  187 bits (476), Expect = 2e-46,   Method: Composition-based stats.
 Identities = 53/226 (23%), Positives = 83/226 (36%), Gaps = 17/226 (7%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKL---GARAHKVL 61
           V+ SGG DS+  + + L++   VH +    G      E+   +     +     R  K+L
Sbjct: 12  VLMSGGLDSSILVGKFLREGRTVHPIYIRTGLFWQSGELPAVQAFLAAIATANLRPLKIL 71

Query: 62  DVTLLNELAVS-SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++ L +      SLT    P  D   EA      ++PGRN L L  AA+Y        + 
Sbjct: 72  ELPLDDLYTDHWSLTGSGTPADDTPDEA-----VYLPGRNALLLVKAAVYCQLHGIGQLA 126

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                   S + D   EF      A++ G +  +    P   + K E   L      + L
Sbjct: 127 CA--PLGSSPFEDASSEFFTEYQSAMNRGSSSPVVLLRPFGELHKEEVMRLG-----VGL 179

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQ 226
               T +C +   G  CG C  C  R         +  TV  A ++
Sbjct: 180 PLELTFSCIHPVDGTHCGTCNKCFERKEAFRITGIEDKTVYTASRR 225


>UniRef50_D2PHM6 ExsB family protein n=3 Tax=Sulfolobus islandicus
           RepID=D2PHM6_SULIS
          Length = 213

 Score =  182 bits (462), Expect = 1e-44,   Method: Composition-based stats.
 Identities = 49/223 (21%), Positives = 87/223 (39%), Gaps = 24/223 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           + + +++FS G DS++      ++  +  C+  +YGQ   R ++   + +  KL  R   
Sbjct: 14  IMKFLLLFSSGLDSSSVAYYYAKKSLDFDCLFINYGQSSVRMQLKYGKIICEKLKRR--- 70

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
              + ++N   +     ++  +  +EP         +  RN++ + +A  YA Q   E V
Sbjct: 71  ---LIIINAKNLGEAFVNNDWLRPHEP---------IIHRNLVIVPIAIAYAKQNGYERV 118

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           I      D    P+ R      +     LG    +RFETP +   K     +     K  
Sbjct: 119 IMATIREDCEYEPNRR----DVMEKLKELGEILGVRFETPFVNFPKYLLLKIG---IKSG 171

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           L  + T +C  G K   CG C+ C  R           PT+  
Sbjct: 172 LDPSLTYSCLLGHKYH-CGKCSQCLKRIEAFKQAKIRDPTIYK 213


>UniRef50_B8GP80 ExsB family protein n=1 Tax=Thioalkalivibrio sp. HL-EbGR7
           RepID=B8GP80_THISH
          Length = 213

 Score =  180 bits (456), Expect = 4e-44,   Method: Composition-based stats.
 Identities = 54/219 (24%), Positives = 85/219 (38%), Gaps = 22/219 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVLD 62
           ++++ SGG +S T L    +Q   V  +  DYGQR    E D AR      G    + LD
Sbjct: 12  SLLLLSGGIESATLLALESRQR-PVTPLVVDYGQRGALRERDAARAQCESHGLTPVE-LD 69

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++         L +               P+  +P RN++ L+LA  YA +   + V+  
Sbjct: 70  LSATGGTFRDGLLKR--------------PHVPLPHRNLVILSLAVSYAARGGIDRVVLA 115

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           +   D + YP     F++    A+   +   +   TPL+  DKA    L      L +  
Sbjct: 116 LNRDDLAAYPSASPGFLEHF-RALIHDLQPGLEITTPLIDRDKAGVIRLGVS---LGVDF 171

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
             T +C  G++   CG C  C  R           PT  
Sbjct: 172 ARTWSCLLGYETH-CGACPQCLHRRAAFRAAGIADPTRY 209


>UniRef50_A8V022 Trans-regulatory protein ExsB n=1 Tax=Hydrogenivirga sp. 128-5-R1-1
           RepID=A8V022_9AQUI
          Length = 166

 Score =  177 bits (449), Expect = 3e-43,   Method: Composition-based stats.
 Identities = 48/171 (28%), Positives = 74/171 (43%), Gaps = 12/171 (7%)

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+  L  +  S+LT + + +P  E   D  P T VP RN+ FL++AA +    + E + 
Sbjct: 2   VDIPHLKGIEGSALTDEKVDIPS-EDYPDQPPITTVPMRNLNFLSIAASFCDVYEIENIG 60

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM------AKDIRFETPLMWIDKAETWALADY 174
            G+   D S YPDCR EF  +    ++            I+  TP + + K +   L   
Sbjct: 61  IGIHSVD-SPYPDCRAEFASSAEAVINASSVMVAKKKNRIKVYTPFLGMTKTDVLKLG-- 117

Query: 175 YGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
             +L +   +T +CY G     CG CA C  R           P V A  +
Sbjct: 118 -LELGVPYEKTYSCYRGTI-PPCGECATCRQRQEAFESVGVKDPLVKADER 166


>UniRef50_Q8ZVX7 7-cyano-7-deazaguanine synthase n=7 Tax=Thermoprotei
           RepID=QUEC_PYRAE
          Length = 269

 Score =  176 bits (447), Expect = 5e-43,   Method: Composition-based stats.
 Identities = 64/246 (26%), Positives = 99/246 (40%), Gaps = 34/246 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLG-------- 54
           R V V SGG DST      L++  +VH ++F YGQ+   E++ A+ L  +L         
Sbjct: 8   RVVAVVSGGPDSTCYTALWLKRGCDVHALSFLYGQKAVVEVERAQLLLRRLDGIAAERGW 67

Query: 55  --ARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
                H+V+D++ L EL   +   D    P    E    P   VP RN++   +A  YAY
Sbjct: 68  GRVVEHRVVDLSSLGELWRGTQLTD----PSVSVEQSYTPTVVVPIRNVVMAAVATAYAY 123

Query: 113 QVKAEA-----VITGVCETDFSG--------YPDCRDEFVKALNHAVSLGM---AKDIRF 156
            V   A     VI G    D           YPDC  E V+A+  A  +      + +  
Sbjct: 124 TVGRNAGAKTYVILGSHYDDIKPREDTWEPLYPDCSPECVEAMQTAFRICHFRGERGVEI 183

Query: 157 ETPLMW-IDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
            TP    + K++   +   + ++  +  +T +CY   +   CG C +C  R         
Sbjct: 184 WTPSREGLRKSQLLKM--CHQEVGDLIYDTWSCYKSGEAH-CGSCESCKNRHAAFIEAGL 240

Query: 216 DKPTVM 221
              T  
Sbjct: 241 PDCTAY 246


>UniRef50_C8PJP9 Queuosine biosynthesis protein QueC n=1 Tax=Campylobacter gracilis
           RM3268 RepID=C8PJP9_9PROT
          Length = 391

 Score =  176 bits (446), Expect = 6e-43,   Method: Composition-based stats.
 Identities = 54/179 (30%), Positives = 87/179 (48%), Gaps = 8/179 (4%)

Query: 48  ELALKLGARAHKVLDVTLLNELAV--SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLT 105
           E++ + GA+  +   ++         S    D I     E     +P+T+VP RN +FL+
Sbjct: 214 EISSQSGAQPQQGDKISSKRAEGEIPSQSQSDKISPSSGESAFSDLPSTYVPFRNGVFLS 273

Query: 106 LAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWI 163
           +AA  A + + +A+  GV + D SGYPDC   F+ A   A+ LG ++D   R +TPL+ +
Sbjct: 274 IAAALAEKERCDAIFIGVVQEDSSGYPDCGASFIAAFERALELGTSRDFHPRIKTPLVHL 333

Query: 164 DKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
            KA+  +LA    ++ +    T +CY   +   CG C +C LR  G     A      A
Sbjct: 334 SKAQIVSLA---LEIGVPLELTWSCYE-REDAACGLCDSCLLRLKGFAGAGAKDKIPYA 388



 Score =  102 bits (255), Expect = 9e-21,   Method: Composition-based stats.
 Identities = 30/93 (32%), Positives = 46/93 (49%), Gaps = 1/93 (1%)

Query: 3  RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVL 61
          RA+ V SGG DS TC   A ++  E+  + FDY QR    E +   ++   LG     +L
Sbjct: 2  RALCVISGGMDSATCAYIARREGYEIVALHFDYDQRTMGKERECFGKICDDLGVAKRLIL 61

Query: 62 DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT 94
          D   + ++  S+LT  ++P+     E  G  NT
Sbjct: 62 DARFIAQIGGSALTDGTLPIRKGAAELCGAANT 94


>UniRef50_Q24ZT5 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Desulfitobacterium
           hafniense Y51 RepID=QUEC1_DESHY
          Length = 220

 Score =  175 bits (445), Expect = 9e-43,   Method: Composition-based stats.
 Identities = 45/211 (21%), Positives = 91/211 (43%), Gaps = 12/211 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKV- 60
           + V++FSGG DST  L   L +  E+  +  +YGQ+ +  E++   ++   L  + + + 
Sbjct: 5   KVVLLFSGGIDSTVLLFWLLSRNYEIFPLFINYGQKSYEGELEAINKILKDLNTKNNLLT 64

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           L++  L  +   +L  +     +     +   + F P RN++ L++AA Y Y+++   + 
Sbjct: 65  LNMPELQLVGSGALVGEY--PKNISSHNEWYASEFFPNRNMILLSIAATYGYKLQISKIA 122

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            GV       Y D    F++A+   ++  +A+      P     + +    A    +L +
Sbjct: 123 IGVVGD---SYQDTTRTFLEAMEMTLAQSIARY-ELIAPFAGHPRQKVIEEAY---RLQV 175

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLN 211
               T +C N      C  C +C  R   + 
Sbjct: 176 PLKSTFSC-NAMGNRHCLLCTSCYEREKAIQ 205


>UniRef50_A9U3Q1 Predicted protein n=1 Tax=Physcomitrella patens subsp. patens
           RepID=A9U3Q1_PHYPA
          Length = 290

 Score =  175 bits (444), Expect = 1e-42,   Method: Composition-based stats.
 Identities = 52/219 (23%), Positives = 82/219 (37%), Gaps = 24/219 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLD 62
           +VV+ SGG +S   L         +H +  +YGQR    E   + ++   LG    ++  
Sbjct: 6   SVVLMSGGIESAVLLYNWRSSSALLHPLYINYGQRGAAMEERASDKICNNLGLELSRI-- 63

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP--GRNILFLTLAAIYAYQVKAEAVI 120
                         D   V +   E      +FVP   RN++ L LAA YA QV +  V 
Sbjct: 64  --------------DMSAVGNKFRELQHGAKSFVPLPHRNLVILGLAASYASQVGSSKVA 109

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G+ + D   Y      F+ +   + +  +   +    PL    KAE   L     +L +
Sbjct: 110 LGLNKDDLGSYSSASQNFMDSF-RSTAASLEPPVTLIAPLSHFTKAEVIRLGK---RLGV 165

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
              +T +C  G +   CG C  C  R          +P 
Sbjct: 166 PFADTYSCMVG-RPVHCGRCHQCRSRQLAFASASEQEPA 203


>UniRef50_A8URJ3 Putative uncharacterized protein n=1 Tax=Hydrogenivirga sp.
           128-5-R1-1 RepID=A8URJ3_9AQUI
          Length = 203

 Score =  175 bits (444), Expect = 1e-42,   Method: Composition-based stats.
 Identities = 52/225 (23%), Positives = 80/225 (35%), Gaps = 23/225 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG-QRHRAEIDVARELALKLGARAHK 59
           MK+  V+FS G +ST+ LV  L++      +    G    R E+  +  L   L  R   
Sbjct: 1   MKKVGVLFSAGVESTSLLVYYLERGYLTLPLYARCGLSWERTELKWSERLWAFLKYRYKN 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++ +                P    +P    I    +P RNI  +  A ++AY      V
Sbjct: 61  LMPLKTF-------------PASGLQPSKGSIE---IPLRNITLVMGAVVHAYVKGIRKV 104

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G        +PD   +++  L   +S G  ++I  ETP M ++K E            
Sbjct: 105 ALG--SLGIYPFPDNNRDYLDRLEALISEGARENISLETPFMGMEKWEVVREFYR----K 158

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
                T +C     G  CG C  C  R  G        PT   + 
Sbjct: 159 APYRLTFSCVKPIIGKHCGKCEKCLERKEGFLKAGVPDPTSYVSS 203


>UniRef50_B9M1I5 ExsB family protein n=4 Tax=Proteobacteria RepID=B9M1I5_GEOSF
          Length = 196

 Score =  175 bits (443), Expect = 2e-42,   Method: Composition-based stats.
 Identities = 65/202 (32%), Positives = 94/202 (46%), Gaps = 16/202 (7%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVLDV 63
           V + SGG DST     A ++    H +  DYGQR R  E+   R    +LG    +V D+
Sbjct: 4   VTLVSGGLDSTLIAKLAQEEGLIQHPLFVDYGQRARDQELAACRRGMSELGLPEPRVADL 63

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
           +    L  S LT  S+ +         + + F PGRN+LFL +AA YA QVKA+A+  G+
Sbjct: 64  SGFGALIHSGLTDSSLDI---------VKDAFTPGRNMLFLLIAAAYAVQVKADAISIGL 114

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
                S +PD    F++     +   M +DI+   PL    K +  ALA           
Sbjct: 115 LHESTSLFPDQTSAFLRDAEVLIRRCMNRDIKLLAPLASFLKTDVVALAKLKR-----IG 169

Query: 184 ETLTCYNGFKGDGCGHCAACNL 205
            T +C+ G + + CG C AC  
Sbjct: 170 ATYSCHTGNE-ETCGTCIACRE 190


>UniRef50_Q13AQ3 ExsB n=6 Tax=Proteobacteria RepID=Q13AQ3_RHOPS
          Length = 206

 Score =  173 bits (439), Expect = 5e-42,   Method: Composition-based stats.
 Identities = 49/212 (23%), Positives = 90/212 (42%), Gaps = 12/212 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLG-ARAHKVL 61
           A+++ SGG DSTT     +++      + FDYGQ     E +  +E+    G       +
Sbjct: 3   ALLLCSGGLDSTTVGYWLVEKNISFQPLFFDYGQHCVEKEWETLQEVLPISGLVSPLLRI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +++ + + + S L    I  P+   E     + ++P R ++F ++ A  A     +AV  
Sbjct: 63  NISEIFQHSPSRL----IAEPNLWTEHVEDDDLYIPYRTLMFFSVGACIAQTRGLDAVYA 118

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G   ++ +   DC   F+  L+          +RFE P     KA+    A+   +L + 
Sbjct: 119 GFINSNHAKELDCSAAFLNNLDSLSR--SLGPVRFELPFRDWSKADVVRRAN---ELRVP 173

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHY 213
             +T +C   F    CG C  C  R + +  +
Sbjct: 174 VGKTYSC-QLFSDTPCGACPNCVERISAIESF 204


>UniRef50_A4YH21 ExsB family protein n=1 Tax=Metallosphaera sedula DSM 5348
           RepID=A4YH21_METS5
          Length = 199

 Score =  167 bits (423), Expect = 3e-40,   Method: Composition-based stats.
 Identities = 55/220 (25%), Positives = 84/220 (38%), Gaps = 26/220 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-IDVARELALKLGARAHKVL 61
           +++ + SGG DST  + +  +   +  C+T DYGQR   E +  AR    KLG R  +V 
Sbjct: 2   KSLFLVSGGLDSTAGMYRFREMDYD--CMTIDYGQRAFKEQVKYARINCEKLGKRLIEV- 58

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                N   V    R+   +  +EP         +  RN + +  A  YA ++   AV  
Sbjct: 59  -----NMKKVGDRFREGKSLIPHEP---------IRHRNAVTIPFALTYASEMGYSAVYV 104

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
                +   Y   + E + AL     L  A  I    P + + KA    +   +G   L 
Sbjct: 105 FTVSEECQ-YESNKPEIISALRK---LAEALRIGLIFPFLGLSKAHVLRMGIEHGMDPL- 159

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              T +C  G +   CG C+ C  R         D  T  
Sbjct: 160 --NTYSCLLGHRAH-CGRCSQCESRKMAFKIAGVDDKTKY 196


>UniRef50_D2L9Z8 ExsB family protein n=1 Tax=Desulfovibrio sp. FW1012B
           RepID=D2L9Z8_9DELT
          Length = 201

 Score =  155 bits (393), Expect = 1e-36,   Method: Composition-based stats.
 Identities = 55/209 (26%), Positives = 91/209 (43%), Gaps = 23/209 (11%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHK 59
           +K + ++ SGG DST+        +      T DYGQ+   AEI  AR++A ++G  +H 
Sbjct: 2   VKNSAILLSGGMDSTSLAFWKKPNF----AFTIDYGQKAAMAEIRAARQVAKEIGI-SHD 56

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT-FVPGRNILFLTLAAIYAYQVKAEA 118
            + +        SSL    + +    P A   P+T + P RN L +TLAA+ A       
Sbjct: 57  TIAIDC------SSLGSGDMAMAAPLPNA---PSTDWWPFRNQLLITLAAMKAICRNINL 107

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           +  G+ ++D S Y D   +FV  ++  +S     +I    P + +  AE   ++     L
Sbjct: 108 LYIGLVKSDSS-YKDGCSDFVDKISRTLSF-QEGNIEVMAPAIDMTAAELVNMSKIPRTL 165

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRA 207
                 + +C+       CG C  CN   
Sbjct: 166 ---LAWSHSCH--VSDFACGRCRGCNKHR 189


>UniRef50_B9ZKA9 ExsB family protein n=1 Tax=Thioalkalivibrio sp. K90mix
           RepID=B9ZKA9_9GAMM
          Length = 217

 Score =  154 bits (390), Expect = 2e-36,   Method: Composition-based stats.
 Identities = 48/218 (22%), Positives = 79/218 (36%), Gaps = 21/218 (9%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLDV 63
           +++ SGG +S+  L Q   Q   +  +  DYGQR    E    R L    G    + +D+
Sbjct: 15  LLLLSGGIESSALLAQRHAQGRPLRALWVDYGQRNAERERAAIRALGADYGVDPVE-MDL 73

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             L +                   ++ + +  +P RN+L L LA   A    A  ++  +
Sbjct: 74  RALRQAFA--------------KRSEWVAHVPLPQRNLLVLALAINLAEHQGAGRILLAL 119

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
              D    P  R  F+  L  A++  +   +  E PL+   KA+          L +  +
Sbjct: 120 NREDQGHGPGSRPSFIGRL-AALADELVPGLAIEAPLIAQSKADII---YTTDPLGIDWS 175

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            T +C    +   CG C  C  R           PT  
Sbjct: 176 RTWSCLL-AQSLHCGRCPQCQARRAAFASAGVPDPTDY 212


>UniRef50_Q989H2 Mll6423 protein n=2 Tax=Proteobacteria RepID=Q989H2_RHILO
          Length = 198

 Score =  150 bits (380), Expect = 3e-35,   Method: Composition-based stats.
 Identities = 57/215 (26%), Positives = 87/215 (40%), Gaps = 22/215 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           M  A+ + SGG DS +              +T DYGQ+   AEID A  +A  LG  +H+
Sbjct: 1   MTTAL-LLSGGMDSVSIAWWLRPD----VALTIDYGQKPAEAEIDAAGHVAQLLGI-SHE 54

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V+ V        SSL   S  +    P+A    + + P RN L +TLA + A +     +
Sbjct: 55  VIRVDC------SSL--GSGDMAGTAPDAAAPVSEWWPFRNQLLVTLAGMAAVRHGVSRL 106

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           + G   TD + + D R EFV+  +  +++     I  E P + +   +   L    G   
Sbjct: 107 LIGALATDGA-HADGRPEFVERASQLMAM-QEGGIVVEAPAIEL---DAVELVRRSGIDQ 161

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYL 214
                  +C+ G     CG C  C    N      
Sbjct: 162 GTLAWAHSCHTGN--YACGRCRGCTKHFNTWQALG 194


>UniRef50_UPI00016C5260 ExsB n=1 Tax=Gemmata obscuriglobus UQM 2246 RepID=UPI00016C5260
          Length = 232

 Score =  146 bits (370), Expect = 4e-34,   Method: Composition-based stats.
 Identities = 52/223 (23%), Positives = 73/223 (32%), Gaps = 19/223 (8%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKLGA---RAHKVL 61
           V+ SGG DS   L +A++    V  +    G      E          + A   R  +VL
Sbjct: 21  VLVSGGLDSAILLAEAVRACPVVFPLYVRSGLHWEAVERAYLERFLAAIAAPNLRPLRVL 80

Query: 62  DVTLLNELAVS-SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV- 119
           D    +      S T + +P  D   EA      F+PGRN+L L    I+       AV 
Sbjct: 81  DQPTADVYGAHWSTTGEGVPGADSADEA-----VFLPGRNVLLLAKPLIWC---HLNAVP 132

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
                    + +PD    F       VS  +  D+R   P   + K +            
Sbjct: 133 FVATAPLGTNPFPDATPAFYDGFAGIVSSAVRGDVRVLRPYAALHKVDVMRRGR-----G 187

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                T +C    +G  CG C  C  R           PT  A
Sbjct: 188 RPLEHTFSCIRPVRGLHCGTCNKCAERRQAFRDAHMTDPTEYA 230


>UniRef50_Q0BZ62 Putative uncharacterized protein n=1 Tax=Hyphomonas neptunium ATCC
           15444 RepID=Q0BZ62_HYPNA
          Length = 188

 Score =  142 bits (358), Expect = 1e-32,   Method: Composition-based stats.
 Identities = 51/207 (24%), Positives = 83/207 (40%), Gaps = 35/207 (16%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID---VARELALKLGARAHKVL 61
           VV+ SGG DS  C             V  DYG  H A I+    A ++   LG    K  
Sbjct: 10  VVLVSGGIDSAACAAMYANNGGSTTGVFVDYG--HPAAIEEFASAEKVCAALGIPLLK-- 65

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                     +S + D I  P             + GRN L +  +A++A  +K   +  
Sbjct: 66  ----------TSFSTDRITGPGE-----------IVGRNALLI-WSAVFASGIKKGVIAI 103

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GV     + Y DC   F+  +++ ++      +R   P +   K+E ++   Y  +  + 
Sbjct: 104 GVHAG--TTYYDCSSTFITRMDNMLAESTNGLVRISAPFVNASKSEVFS---YLQRSKVE 158

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRAN 208
            + T +C +G K + CG CA+C  R  
Sbjct: 159 ASLTYSCESGSK-EPCGICASCQDRTK 184


>UniRef50_C7RWC1 ExsB family protein n=4 Tax=Proteobacteria RepID=C7RWC1_9PROT
          Length = 201

 Score =  136 bits (343), Expect = 6e-31,   Method: Composition-based stats.
 Identities = 49/214 (22%), Positives = 80/214 (37%), Gaps = 21/214 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLD 62
           A ++ SGG DS +              +T DYGQ    AEI  ++ +  +L    H VL 
Sbjct: 3   AALLLSGGMDSISIAWWKHPD----IAITIDYGQLPAAAEITASQAICRRLRI-PHHVLQ 57

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V   +          S  +   EP A      + P RN L +TLAA+ A  ++   +  G
Sbjct: 58  VDCHSL--------GSGDMAGLEPNALAPATDWWPYRNQLLITLAAMKAVTLEVRQLWLG 109

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
              +D S + D    F+ A++  +++     +    P + +    T  L    G    + 
Sbjct: 110 TVASDAS-HRDGTPNFLSAMSALLAV-QEGGLTVSAPAIGMS---TVDLVRQSGVPLEIL 164

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
               +C+       CG C  CN     L     +
Sbjct: 165 AWAHSCHKA--DVACGACRGCNKYFEVLRELGNE 196


>UniRef50_B7GTM4 ExsB family protein n=2 Tax=Actinobacteridae RepID=B7GTM4_BIFLI
          Length = 251

 Score =  133 bits (335), Expect = 5e-30,   Method: Composition-based stats.
 Identities = 49/231 (21%), Positives = 78/231 (33%), Gaps = 23/231 (9%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVL 61
           R  ++ SGG DSTT L   L Q  E++ VTF++G+     E   A+ +   L +      
Sbjct: 7   RVALLLSGGPDSTTLLYDLLNQGKEIYAVTFNFGETEGCNESQHAQRIVTNLHSERLHHY 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFV---------PGRNILFLTLAAIYAY 112
                     S   +    +P  +          V         P  + + L L A +A 
Sbjct: 67  QYDF------SEQFKRFYELPQPQFLRKAFGPFVVAPNKEEHVQPFGSAIALMLMASWAL 120

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVS--LGMAKDIRFETPLMWIDKAETWA 170
           +     V   V   D + + D    + +AL+       G    + F TP + I+K     
Sbjct: 121 KHDIVDVYYAVHSND-AVFHDNHASYFEALHTVTVECEGDEYGVTFHTPYLSINKCAVIQ 179

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                  L +   +T +C  G     CG C  C  R           P + 
Sbjct: 180 KG---MGLGVPFGDTWSCALGGTVH-CGVCDPCRDRRTSFEILGIKDPVLY 226


>UniRef50_B9LWH7 ExsB family protein n=1 Tax=Halorubrum lacusprofundi ATCC 49239
           RepID=B9LWH7_HALLT
          Length = 195

 Score =  126 bits (318), Expect = 4e-28,   Method: Composition-based stats.
 Identities = 50/209 (23%), Positives = 81/209 (38%), Gaps = 21/209 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           M  +V++ SGG DS T              VT DYGQ    AEI  + ++A +L    H+
Sbjct: 1   MPESVLLLSGGLDSATLAYTHRPD----LTVTVDYGQCCADAEIQASAQIADQLNLE-HE 55

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V++V   N  A +        +   +    G    + P RN L +TL A+   +  A+ +
Sbjct: 56  VIEVDCENLGAGT--------MAGQQETDLGDAPEWWPYRNQLVVTLVAMKVVRRGADRL 107

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           + G    D S + D   EF + ++  +S     D+    P +     E           +
Sbjct: 108 LVGAVADDQS-HADGEAEFFELMDSLLSF-QEGDLGVVAPAIDRTTEEFV---CEVNPPE 162

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRAN 208
            +   T +C        CG C +C  R  
Sbjct: 163 SLLGWTHSC--TASNTACGECRSCKKRQR 189


>UniRef50_Q2RGB7 ExsB n=1 Tax=Moorella thermoacetica ATCC 39073 RepID=Q2RGB7_MOOTA
          Length = 192

 Score =  126 bits (317), Expect = 6e-28,   Method: Composition-based stats.
 Identities = 40/214 (18%), Positives = 69/214 (32%), Gaps = 29/214 (13%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M   +V+ SGG DS   +     +   V+ +   YGQ         RE        AH  
Sbjct: 1   MNPVLVLLSGGIDSAALVHYYRSKNTSVYGLHVQYGQPV-----AEREAQAAKAIAAHYG 55

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+ + +           I  P      + +       RN +F+ +A           V 
Sbjct: 56  IDLRIAH-----------IQPPLARKGEEYL------CRNAIFVLIACANLSP-GINRVA 97

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G+     + Y DC   F+  +   +    +  +  E P +   K E +    Y     +
Sbjct: 98  LGIHSG--TIYYDCSQGFMADMQRLLDGYFSGAVTVEAPFLRWSKLEVYQ---YCTSHGV 152

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYL 214
             + T +C      + CG C +C  R        
Sbjct: 153 PVDLTYSCER-NNDEPCGQCPSCYDRRRLYEAGG 185


>UniRef50_B1ZBL1 ExsB family protein n=4 Tax=Proteobacteria RepID=B1ZBL1_METPB
          Length = 214

 Score =  119 bits (298), Expect = 1e-25,   Method: Composition-based stats.
 Identities = 51/213 (23%), Positives = 73/213 (34%), Gaps = 22/213 (10%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLDV 63
           V++FSGG DST              C+  DYGQR    E   A  +A + G R       
Sbjct: 3   VLLFSGGLDSTALAWWQRPD----ACLFVDYGQRPAAGERAAATSIAAEFGLR------- 51

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             L  L V        P+   EP A      + P RN + +TLA +         ++ G 
Sbjct: 52  --LETLQVDLSGLGLGPLAGGEPAALARAPEWWPYRNQILVTLAGMRFVAEGLSEILIGT 109

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
             TD   + D R  F++ L+  + L     +R   P   +       LA           
Sbjct: 110 VATD--VHADGRAPFLRTLDRLMRL-QEGGVRVVAPARHLSGP--ALLAASGLPASA-LG 163

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
            T +C+       CG C  C      L  +   
Sbjct: 164 ATFSCH--VMEYACGQCRGCEKHRETLRSFGLR 194


>UniRef50_C3NEW9 ExsB family protein n=5 Tax=Sulfolobus RepID=C3NEW9_SULIY
          Length = 244

 Score =  114 bits (285), Expect = 3e-24,   Method: Composition-based stats.
 Identities = 43/221 (19%), Positives = 78/221 (35%), Gaps = 37/221 (16%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG---QRHRAEIDVARELALKLGARAHKV 60
           A++  +GG DST        +Y  +H     YG   Q    E+++ +EL+ KL     K+
Sbjct: 5   AIIQLTGGIDSTVLAYYLKDKY-VLHGTFIYYGYPPQ--IRELELVKELSKKLDV-PLKI 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D          S    S  +P  +     I   FV       + + A ++       + 
Sbjct: 61  IDF---------SSYIKSFDLPPIDSIQYIIKYQFV-------IEILASFSAYPNLNTMF 104

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G  + ++S     +   +K +   +        +   P   + K+E   L +   +L +
Sbjct: 105 VGWLKDEWS----NKMSILKGIESLM------GFKVMAPFHTMVKSEVIKLGN---ELGV 151

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              +T +C    K   CG C  C  R           PT+ 
Sbjct: 152 DFTKTHSCIVSGKMH-CGICMPCRSRRRAFEEAKVKDPTIY 191


>UniRef50_Q12C85 ExsB n=1 Tax=Polaromonas sp. JS666 RepID=Q12C85_POLSJ
          Length = 282

 Score =  112 bits (281), Expect = 9e-24,   Method: Composition-based stats.
 Identities = 54/240 (22%), Positives = 87/240 (36%), Gaps = 29/240 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVL 61
           +A+VVFSGG DS    + A  +  +   +TF + QR +  E+  +  LA  LG    +++
Sbjct: 32  KAIVVFSGGPDSLAAALWAKSKKYDPWLLTFQFKQREQGGEMFASMLLAKTLGF-PQEII 90

Query: 62  DVTLLN-------ELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
           D             + + + T  +     +     G           + +  A  +A   
Sbjct: 91  DFKSPMHSFEPTVHVLMHAGTTTTADRAQHHRMEFGA---------GMVMATACTFAVYH 141

Query: 115 KAEAVITGVCETDFSG----YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
           + + V+ G  + D  G    Y     EF  A  + VS    +  R   P     K +   
Sbjct: 142 QIDDVVWGATKDDVFGGRFEY---SQEFADAFANLVSCTTGRPFRIHVPFASKSKHQV-- 196

Query: 171 LADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTGL 230
           LA++ GK DL    + +C  G     CG C AC  R            T       K+ L
Sbjct: 197 LAEFSGKEDL-FAMSWSCKVGSSIQ-CGTCTACVARRVAAQASGVKDATSYHEQNYKSPL 254


>UniRef50_C6NX81 Archaeosine biosynthesis protein queC n=1 Tax=Acidithiobacillus
           caldus ATCC 51756 RepID=C6NX81_9GAMM
          Length = 206

 Score =  109 bits (273), Expect = 8e-23,   Method: Composition-based stats.
 Identities = 47/215 (21%), Positives = 73/215 (33%), Gaps = 24/215 (11%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-IDVARELALKLGARAHKV 60
           +R++++ SGG +S T           ++    DYGQR  AE     + LA  L     + 
Sbjct: 5   ERSLLLCSGGVESFTLTHAFGTDSG-LYLFFLDYGQRAAAEEWKRVQNLARNLRLERERF 63

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                        L      V    P+   +P    P RN+L +  AA  A  +    V+
Sbjct: 64  ------------DLRDFGDAVGRLRPQRYHVPT---PHRNLLAVAAAANVAESLGRNTVV 108

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G    D     +    F+ AL   +S   +  +    PL    KA    L      L +
Sbjct: 109 LGTTADDGRTDRNANRAFLSALAATLS---SSGLYLALPLAQYRKAAVIRLGQD---LGV 162

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
               + +C  G++   CG C  C  R         
Sbjct: 163 PWEMSYSCIVGYEAQ-CGRCPQCIRRREAFREAGL 196


>UniRef50_A1SFM8 ExsB family protein n=1 Tax=Nocardioides sp. JS614
           RepID=A1SFM8_NOCSJ
          Length = 183

 Score =  106 bits (266), Expect = 4e-22,   Method: Composition-based stats.
 Identities = 46/203 (22%), Positives = 66/203 (32%), Gaps = 36/203 (17%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLDVT 64
           V+ SGG DS   L     +      V  DYGQ     E   +R +A   G+   +V+   
Sbjct: 10  VLLSGGVDSALVLAMLSNRSP--VSVWVDYGQPVADVERQASRAIANHFGSPWMEVVIKG 67

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
           L    +   L                       GRN L +  A   A       V  G  
Sbjct: 68  LAPPASGEFL-----------------------GRNDLLVAAALSVA---GGGFVAIGTH 101

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNE 184
               +GY DC   +  +    +            PL  +DKA+ + LA   G   +    
Sbjct: 102 AG--TGYADCSLAWAGSWQALLDAQHGGTAGLLAPLSNLDKAQVYELAVANG---VPIEL 156

Query: 185 TLTCYNGFKGDGCGHCAACNLRA 207
           T +C        CG C++C  R 
Sbjct: 157 THSC--ETTAAPCGSCSSCADRR 177


>UniRef50_O67015 Putative uncharacterized protein n=1 Tax=Aquifex aeolicus
           RepID=O67015_AQUAE
          Length = 110

 Score =  106 bits (264), Expect = 8e-22,   Method: Composition-based stats.
 Identities = 29/112 (25%), Positives = 44/112 (39%), Gaps = 6/112 (5%)

Query: 99  RNILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFET 158
           RN +  T  A+Y           G        + D + EF + L+  +S+ + K+   ET
Sbjct: 2   RNFILCTAGALYGVPKGINTCAIG--SLGMYPFEDNKKEFFEKLSETISMSLGKEFYIET 59

Query: 159 PLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
           P M + K E       +    +    +LTC N   G+ CG C  C  R   L
Sbjct: 60  PFMGMHKHEVIKRYGKF----IPLELSLTCINPVNGEPCGKCIKCKEREEAL 107


>UniRef50_B4U7S9 ExsB family protein n=1 Tax=Hydrogenobaculum sp. Y04AAS1
           RepID=B4U7S9_HYDS0
          Length = 196

 Score =  104 bits (259), Expect = 3e-21,   Method: Composition-based stats.
 Identities = 39/204 (19%), Positives = 75/204 (36%), Gaps = 16/204 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG-QRHRAEIDVARELALKLGARAHKVL 61
           + V + S G DS + L   + +  E++ +    G +    E+   +++   +      + 
Sbjct: 2   KVVSLVSSGFDSISLLNYYINKNYEIYPIYVKSGFKWEEEELKHLKDIIDHISKSYKLIK 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            + +       S   +       E       +  +P RN+  L  A  YAY V A  +  
Sbjct: 62  PLKV-------SHAPNDFFEDFNERAFINDEDVEIPFRNLDLLISALKYAYAVDANLIAI 114

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+       + D    F+K +N   S  M  D + +TP + + K + +           +
Sbjct: 115 GI--MGLVAFKDNSYNFIKTINDLFS--MYGDYKVDTPFLGMSKKDVFDRYFI----KEL 166

Query: 182 RNETLTCYNGFKGDGCGHCAACNL 205
            ++   C N   G  CG C+ C  
Sbjct: 167 FDKAFCCMNPTDGKECGVCSKCKE 190


>UniRef50_C5PNW4 ExsB superfamily protein n=1 Tax=Sphingobacterium spiritivorum ATCC
           33861 RepID=C5PNW4_9SPHI
          Length = 198

 Score =  102 bits (255), Expect = 9e-21,   Method: Composition-based stats.
 Identities = 41/207 (19%), Positives = 73/207 (35%), Gaps = 21/207 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKLGARAHKVL 61
           +  ++ SGG DS                 T +YGQ     EI V+R +   L    HKV+
Sbjct: 5   KQGLLLSGGVDSICLAYGLKPD----IAYTINYGQIPASREIYVSRYICNLLRI-QHKVI 59

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +V   +  +   +  + + +   +         + P RN L +TLAA+ A + K   +  
Sbjct: 60  EVNCKSLGSGILINEERLDISPSQE--------WWPYRNQLLITLAAMQAIKDKIGVLHL 111

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              ++D S + D   +F   +N  V      +I            E     +   +   +
Sbjct: 112 ASVKSD-SFHKDGTPQFYDLINELVKY-QEGNISIVCESTGYYSHELVKKYNVPLE---I 166

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRAN 208
                +C+       CG C  C  +  
Sbjct: 167 IAFAHSCH--ISNTACGQCPGCKKQIK 191


>UniRef50_C9YE01 Putative uncharacterized protein n=1 Tax=Curvibacter putative
          symbiont of Hydra magnipapillata RepID=C9YE01_9BURK
          Length = 143

 Score = 84.1 bits (207), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 36/82 (43%), Positives = 50/82 (60%), Gaps = 13/82 (15%)

Query: 4  AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV----AREL-------ALK 52
          A+V+FSGGQDSTTCL  AL +Y+ V  + FDY QRH  E++      RE+       A K
Sbjct: 5  ALVLFSGGQDSTTCLAHALARYERVETIAFDYRQRHNVELEARLNVLREIKTRFPQWAAK 64

Query: 53 LGARAHKVLDVTLLNELAVSSL 74
          LG     +LD+ +L E++ +SL
Sbjct: 65 LG--EDHLLDLAVLGEVSETSL 84


>UniRef50_B8JF98 PP-loop superfamily ATPase-like protein n=1 Tax=Anaeromyxobacter
           dehalogenans 2CP-1 RepID=B8JF98_ANAD2
          Length = 447

 Score = 79.5 bits (195), Expect = 9e-14,   Method: Composition-based stats.
 Identities = 42/236 (17%), Positives = 71/236 (30%), Gaps = 39/236 (16%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V +FSGG DS        +   ++  V+      H  ++  + +       +      V 
Sbjct: 142 VGLFSGGLDSIVGTYLRSRTAKKLIVVSV-----HGIQVRASAQQRAITMLQRASFFSVP 196

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
           L        L     P                  R  LFL++ A  A  V A  + T   
Sbjct: 197 LHWVSFDHQLRHAHPPEKSQR------------CRGFLFLSIGAAVARTVGASTLHTYEN 244

Query: 125 ETDFSGYP---------DCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                  P         + R      + +L   + +     +R E P ++  K E    A
Sbjct: 245 GIGALNPPMNLAQVGAQNTRAMHPGTLASLEGILEVVQDDPVRLEAPFLFSTKGEMCRDA 304

Query: 173 DYYGKLDLVRNE-TLTCYNGFKG-----DGCGHCAACNLRANG-LNHYLADKPTVM 221
             +    +   + + +C  G  G     + CG C +C LR +          PT  
Sbjct: 305 REHL---IPLAQASNSCDEGEHGKPDPAEHCGLCTSCLLRRSALFAALGDKDPTPY 357


>UniRef50_A8L9C0 ExsB family protein n=2 Tax=Actinomycetales RepID=A8L9C0_FRASN
          Length = 372

 Score = 79.1 bits (194), Expect = 1e-13,   Method: Composition-based stats.
 Identities = 40/215 (18%), Positives = 72/215 (33%), Gaps = 32/215 (14%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V++FS G DS      A     +   + FD G R+R +   A                  
Sbjct: 132 VLLFSAGLDS----FPAWHYLGKPPALYFDLGHRYRDQERAA---------------ITA 172

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
           L++   +       + +  +E +        +P RN+    LAA  A ++    V     
Sbjct: 173 LVDRCGIKVTVSQELDLSQWEADD-----AIIPLRNVYLAMLAANRAERIWCVGV----- 222

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNE 184
           + D +   D   +    ++  ++    + +  ++P   + K E  A     G        
Sbjct: 223 KGDATA--DKSPQAFHQISQMITDLSGRPVWLDSPFWQMTKTEIVAWYLGQGLPAGDLLL 280

Query: 185 TLTCYNGFKG-DGCGHCAACNLRANGLNHYLADKP 218
           T +C         CG C +C  R   L +   D P
Sbjct: 281 TFSCSRTDSAMTHCGQCPSCLRRWISLINNGIDAP 315


>UniRef50_Q9RZH7 Putative uncharacterized protein n=1 Tax=Deinococcus radiodurans
           RepID=Q9RZH7_DEIRA
          Length = 423

 Score = 76.4 bits (187), Expect = 7e-13,   Method: Composition-based stats.
 Identities = 44/237 (18%), Positives = 70/237 (29%), Gaps = 46/237 (19%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V + SGG DS   ++  + +      V+    Q    +    RE+A  LG   H  L + 
Sbjct: 140 VALLSGGLDSLVGMIDLVAEGHNPLAVS----QLVAGDAAKQREIATDLGRTTH--LQLN 193

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA------YQVKAEA 118
              E                 P  D   +     R ++FL  A + A       Q +A  
Sbjct: 194 HAAECPT--------------PGEDSQRS-----RTLIFLAYAVLAATATTRFQQARAVP 234

Query: 119 VIT---GVCETDFSGYP------DCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKA 166
           +     G    +    P        R     F++ L   +   +  ++    P     K 
Sbjct: 235 IYINENGFIALNPPLTPMRIGSLSTRTAHPVFLQQLQAVLQ-AVGLNVALINPYALKTKG 293

Query: 167 ETWALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           E          L    + T +C  Y  F    CG C  C +R      +     T  
Sbjct: 294 EMLTECANQTVLSRWASATTSCGRYRRFGYKHCGRCIPCQVRRAAFLAWGQPDRTEY 350


>UniRef50_UPI0001B54B61 PP-loop superfamily ATPase-like protein n=1 Tax=Streptomyces sp.
           AA4 RepID=UPI0001B54B61
          Length = 423

 Score = 74.9 bits (183), Expect = 2e-12,   Method: Composition-based stats.
 Identities = 45/224 (20%), Positives = 72/224 (32%), Gaps = 39/224 (17%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVA---RELALKLGARAHKVLD 62
           V+FSGG DS+  L   L     +  V+       R   D+A     +  +L   + +VL 
Sbjct: 118 VLFSGGLDSSAGLALRL-GGPPLLPVSV------RTNNDMAGVQERVLSRLRGVSREVLP 170

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI-- 120
                     +LT      P  + E           R +LFL      A  +    +   
Sbjct: 171 AASFRV----NLTESRTRAPGAKQENSQRS------RGLLFLAAGIAVAITLGKGRLWFL 220

Query: 121 ---TGVC-------ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
               G         +             V+A+         +D   ETPL+ + KAE  +
Sbjct: 221 ENGIGAINLPYLRSQVGSQATRSAHPRTVQAMARLAGTLTGQDFAVETPLLGLTKAEAVS 280

Query: 171 LADYYGKLDLVRNETLTCYNGFKGD-----GCGHCAACNLRANG 209
                 + +     +++C  GF         CG C +C LR   
Sbjct: 281 --AVPPEYEHAIACSVSCDTGFASRISGHPPCGTCTSCLLRRQA 322


>UniRef50_C9Q119 Putative uncharacterized protein n=1 Tax=Prevotella sp. oral taxon
           472 str. F0295 RepID=C9Q119_9BACT
          Length = 432

 Score = 72.5 bits (177), Expect = 1e-11,   Method: Composition-based stats.
 Identities = 45/247 (18%), Positives = 77/247 (31%), Gaps = 43/247 (17%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHKVLDVT 64
            + SGG DS    +  L+   E   +    YG                 G +  KV   +
Sbjct: 156 CMLSGGLDSFIGAINLLKDGKE--PIFVSHYG-----------------GGKGVKVYQDS 196

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE-AVIT-- 121
           ++N L +            Y    +G  +T    R+++F   A + A  +     +    
Sbjct: 197 VVNSLKIQYAVDSKRFFRFYAASQNGAEDTT-RSRSLMFFAHAIVLASGIGHHVDLYVPE 255

Query: 122 -GVCETDFS------GYPDCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
            GV   +        G    R     F+      +  G+  DI    P     K E    
Sbjct: 256 NGVISLNIPLTVMRLGSLSTRTTHPYFMGMFQKLL-TGLDIDISLVNPFQMQTKGEMMKT 314

Query: 172 ADYYGKLDLVRNETLTCYNGFKGD-------GCGHCAACNLRANGLNHYLADKPTVMA-A 223
                 L+    +TL+C +  +G         CG C  C +R   +     +  T     
Sbjct: 315 CKDLDFLNAHYQDTLSCSHPDQGRWTGDAVGHCGECLPCTIRKAAIKAAGLEDVTTYRHH 374

Query: 224 MKQKTGL 230
            + +TG+
Sbjct: 375 YETETGI 381


>UniRef50_A3CVZ1 ExsB family protein n=1 Tax=Methanoculleus marisnigri JR1
           RepID=A3CVZ1_METMJ
          Length = 257

 Score = 71.8 bits (175), Expect = 2e-11,   Method: Composition-based stats.
 Identities = 37/175 (21%), Positives = 59/175 (33%), Gaps = 33/175 (18%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
             V  SGG DS+  L  A     +V  ++ D G     E+  AR+L+ +LG   H V+ +
Sbjct: 17  VAVALSGGTDSSVLLALARHHGIQVIAISVDTGLTPPGELAAARKLSERLGL-PHVVIPL 75

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            +L              +P            +V  R  +   +    A +     V+ G 
Sbjct: 76  DML-------------DIPAVRENRPD--RCYVCKR-AMMEAI-VAEARRQGCRTVVDGT 118

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMW--IDKAETWALADYYG 176
              D       R      +     LG       ++P     + KA+  ALA   G
Sbjct: 119 HADD-------RPAARPGMRVLSELG------IKSPFAECGMGKADVEALARELG 160


>UniRef50_UPI0000382ADB COG0603: Predicted PP-loop superfamily ATPase n=1
           Tax=Magnetospirillum magnetotacticum MS-1
           RepID=UPI0000382ADB
          Length = 178

 Score = 71.4 bits (174), Expect = 2e-11,   Method: Composition-based stats.
 Identities = 35/169 (20%), Positives = 56/169 (33%), Gaps = 28/169 (16%)

Query: 40  RAEIDVARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGR 99
           R E+D AR+ A +L A     L +        S L R                  + P R
Sbjct: 14  RRELDFARDRAGRLSA-----LALARSRGAQASDLAR---------------APEWWPYR 53

Query: 100 NILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETP 159
           N + +TLA +         ++ G   TD   + D R  F++ ++  + L     +R   P
Sbjct: 54  NQMLVTLAGMRFVAEGLTEILLGAIRTD--VHADGRAPFLRTVDRLMHL-QEGGVRVMAP 110

Query: 160 LMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRAN 208
              +   E    +     L      T +C+       CG C  C     
Sbjct: 111 ARHLSALELVRRSGIPRSL---LGATFSCH--VMEYACGRCRGCEKHQE 154


>UniRef50_Q9V1S6 ThiI thiamin biosynthesis protein thiI n=3 Tax=Pyrococcus
           RepID=Q9V1S6_PYRAB
          Length = 198

 Score = 67.2 bits (163), Expect = 4e-10,   Method: Composition-based stats.
 Identities = 34/183 (18%), Positives = 66/183 (36%), Gaps = 20/183 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + S G DS   +     +  EV+ + F   Q    E  V R +         KV D
Sbjct: 2   KVVALLSSGIDSPVAIHLVASKGAEVYPLHFK--QDDVKENKVRRIVKRLQEIHGDKVKD 59

Query: 63  VTLLNELA-VSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
             +++       +      +   +        T           +A  YA ++KA+A++T
Sbjct: 60  PRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYT--------MYIVATSYAREIKAKAIVT 111

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G      +          + L++ + +  A ++    PL+ +DK E   +A   G  D+ 
Sbjct: 112 GDSLGQVAS---------QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDIS 162

Query: 182 RNE 184
             +
Sbjct: 163 TEK 165


>UniRef50_A9GG06 Putative uncharacterized protein n=1 Tax=Sorangium cellulosum 'So
           ce 56' RepID=A9GG06_SORC5
          Length = 435

 Score = 67.2 bits (163), Expect = 4e-10,   Method: Composition-based stats.
 Identities = 48/249 (19%), Positives = 87/249 (34%), Gaps = 37/249 (14%)

Query: 4   AVVVFSGGQDSTTCLVQALQ--QYDEVHCVTFDYGQRHRAEIDV--ARELALKLGARAHK 59
           AV +FSGG DS    +  L+  +   V  V   Y   H        A EL  + G    +
Sbjct: 117 AVCLFSGGVDSLIGAINLLESDRKSRVVLVG-HYDSTHTPGTQKVLADELIQEYGDE--R 173

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK-AEA 118
           VL + +L  +  + +       P    +     +     R+++FL L    A  +  A  
Sbjct: 174 VLPLRVL--VRPAPIRDGVQAYPLPAVKESTTRS-----RSLVFLGLGMAVAAALGPAVP 226

Query: 119 VIT---GVCETD----FSGYPDCR-----DEFVKALNHAVSL-GMAKDIRFETPLMWIDK 165
           +     G    +     S    C        F+  + +A+ + G+   I    P ++  K
Sbjct: 227 LYIPENGFIAINVPLHGSRVGSCSTRTTHPYFLDRIANALGVVGVKNPI--VNPYVYKTK 284

Query: 166 AETWALADYYGKLDLVRNETLTCYNGFKGDG-------CGHCAACNLRANGLNHYLADKP 218
            E          L  V + T++C +  +          CG+C  C +R    N    +  
Sbjct: 285 GEMVRECKNQDLLRRVAHITVSCAHPERKQNETNTWPNCGYCYPCLIRRAAFNWAGFESS 344

Query: 219 TVMAAMKQK 227
           ++ A  ++ 
Sbjct: 345 SMYARYRRD 353


>UniRef50_Q4UTY2 Putative uncharacterized protein n=2 Tax=Gammaproteobacteria
           RepID=Q4UTY2_XANC8
          Length = 428

 Score = 66.8 bits (162), Expect = 6e-10,   Method: Composition-based stats.
 Identities = 36/240 (15%), Positives = 65/240 (27%), Gaps = 45/240 (18%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + +V + SGG DS    +   +       V+                    +    H   
Sbjct: 141 EDSVCLLSGGMDSLIGAIDLAESGRRSIAVS------------------NTVPGDGHNQH 182

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA--- 118
                       L  ++ P  D   E           R+I+FL    + A  ++A     
Sbjct: 183 SFAEAVGFKHLPLNHNASPPSDGSREPSQR------ARSIIFLAFGVLAATSLQAYQDGA 236

Query: 119 ---VIT---GVCETDFS------GYPDCR---DEFVKALNHAVSLGMAKDIRFETPLMWI 163
              +     G    +        G    R    EF+  +   +       +    P    
Sbjct: 237 QVPLFICENGFIALNPPLTGTRLGSLSTRTAHPEFLHGVQSVLD-AAGLRVNLINPYQHK 295

Query: 164 DKAETWALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            K E          L  +   +++C  Y  +  + CG C  C +R +    +    PT  
Sbjct: 296 TKGEMSRECADQDLLRNLAASSVSCGRYRRYGYNQCGRCIPCQIRRSAYLAWGQPDPTDY 355


>UniRef50_C6ALB1 Putative uncharacterized protein n=2 Tax=Proteobacteria
           RepID=C6ALB1_AGGAN
          Length = 438

 Score = 66.4 bits (161), Expect = 8e-10,   Method: Composition-based stats.
 Identities = 42/241 (17%), Positives = 81/241 (33%), Gaps = 42/241 (17%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDY-GQRHRAEIDVARELALKLGARAHKV 60
           K  V +FSGG DS    +  L+Q      V+  Y G + R      + +  +L    +  
Sbjct: 147 KDCVCLFSGGLDSAIGTIDLLEQGHSPVLVSHSYKGDKSRQ-----QAIIQQLNQNGY-- 199

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRN--ILFLTLAAIYAYQVK--- 115
             +   ++    +           +P  +   +T +  R   + FL  A + AY ++   
Sbjct: 200 --INQFSQFNAIA-----------QPHLNNGKSTEITMRTRSLNFLAFAIVSAYALQKVA 246

Query: 116 ---AEAVIT--GVCETDFS------GYPDCR---DEFVKALNHAVSLGMAKDIRFETPLM 161
               + ++   GV   +        G    R     F++ +       +        P  
Sbjct: 247 QKKIDILVPENGVISINAPLTVRRVGTLSTRTTHPYFIQEIQKLF-TAVNIPFTLRNPYQ 305

Query: 162 WIDKAETWALADYYGKLDLVRNETLTC-YNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
           +  K +  A       L  +  +T++C +   K   CG C  C +R   L++      T 
Sbjct: 306 FKTKGQMIAECRNLPLLQQIIPDTVSCSHWKRKNQQCGVCVPCLIRRASLHYAGITNDTQ 365

Query: 221 M 221
            
Sbjct: 366 Y 366


>UniRef50_B5CVE8 Putative uncharacterized protein n=1 Tax=Bacteroides plebeius DSM
           17135 RepID=B5CVE8_9BACE
          Length = 233

 Score = 66.4 bits (161), Expect = 8e-10,   Method: Composition-based stats.
 Identities = 34/225 (15%), Positives = 67/225 (29%), Gaps = 51/225 (22%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHC-VTFDYGQRHRAEIDVARELALKLGARAHKV 60
           ++ V+++SGG DS       + +  +    +  D G ++  E         ++      V
Sbjct: 7   EKKVLLYSGGMDS-----WLIDKIWKPDVKLYVDMGTKYSQE---------EIKRLPADV 52

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +               + + +  +E E        +P RN+  + +A  Y        + 
Sbjct: 53  V--------------VEKLDLSKWEREDK-----IIPLRNMYLIGIATNYG-----NEIC 88

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSL-------GMAKDIRFETPLMWIDKAETWA--L 171
            G    D     D    F       ++           + I+         KAE     +
Sbjct: 89  LGATAGDRVL--DKSPVFADIYEQLLNYLYQKQHWTEERKIKINLDFKRYTKAELVRAFI 146

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
           A   G +D   + + +CY    G  C  C  C  +          
Sbjct: 147 AQ-GGDMDEAFSSSFSCYTPEHGKECWSCKPCFRKFIAFALNGYP 190


>UniRef50_Q9UWQ2 Putative thiamin biosynthesis protein thiI n=1 Tax=Pyrococcus
           furiosus RepID=Q9UWQ2_PYRFU
          Length = 363

 Score = 66.0 bits (160), Expect = 8e-10,   Method: Composition-based stats.
 Identities = 34/191 (17%), Positives = 62/191 (32%), Gaps = 20/191 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + SGG DS       L++  EV  + FD G   +  ++   E+         +++ 
Sbjct: 167 KVVALISGGIDSPVAAFLMLKRGAEVIALHFDQGTNAKKVVEKVVEILSDYSPEPIELIV 226

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                 L         +   ++      I            L  AA  A +  A  ++TG
Sbjct: 227 ENHFEILKPYVSVLAKLQKREWTCILCKIA----------MLKRAAEIAKEEGALGIVTG 276

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L++     ++ D     PL+  DK E  ++A   G  D   
Sbjct: 277 DSLGQVAS---------QTLSNLFIETISVDYPIYRPLIGFDKEEIVSIAKRIGTYDAFL 327

Query: 183 NETLTCYNGFK 193
                C   F+
Sbjct: 328 EYPY-CECPFR 337


>UniRef50_C5CE34 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Kosmotoga olearia TBF 19.5.1 RepID=C5CE34_KOSOT
          Length = 404

 Score = 66.0 bits (160), Expect = 1e-09,   Method: Composition-based stats.
 Identities = 38/177 (21%), Positives = 69/177 (38%), Gaps = 28/177 (15%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---Y-GQRH-RAEIDVARELALKLGARAH 58
           A+++ SGG DS      AL++   +H + F    Y G++     +D+A+ L    G R  
Sbjct: 177 ALLLLSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDM 236

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            +  V           T+  + +     E+  +    V  R  +   +A   A +   +A
Sbjct: 237 LLYRVHF---------TKLQLAIHRNVRESLSL----VIQRRAMM-RIATKIAKEHGYKA 282

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
           ++TG      +          + + +  ++G A DI    PL   DK ET  +A   
Sbjct: 283 IVTGENLGQVAS---------QTIENLHTIGSATDILVLRPLTGFDKIETIQIAKKI 330


>UniRef50_Q8R5Z5 tRNA-specific 2-thiouridylase mnmA 2 n=12 Tax=Fusobacterium
           RepID=MNMA2_FUSNN
          Length = 343

 Score = 65.2 bits (158), Expect = 2e-09,   Method: Composition-based stats.
 Identities = 45/241 (18%), Positives = 77/241 (31%), Gaps = 63/241 (26%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARA 57
           MK+ V+  SGG DS+       +Q  EV  +T +  Q    +  +I+  +++  KLG   
Sbjct: 1   MKKVVIGMSGGVDSSVSAYLLKEQGYEVIGITLN--QHLEENSKDIEDTKKVCDKLGII- 57

Query: 58  HKVLDV-----TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
           H+V+++      ++ +  +        P P    + +            +   +    A 
Sbjct: 58  HEVVNIRKDFENIVIKYFLEGYNSGKTPSPCIICDDE------------IKFKILFDIAD 105

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNH----------------AVSLGMAKDIRF 156
           +  AE V TG   T          E+ +  +                    L   K  R 
Sbjct: 106 KYNAEYVATG-HYT--------SVEYSEVFSKYLLKSVHSIIKDQSYMLYRLSPDKLERL 156

Query: 157 ETPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
             PL    K E   +A     L +            K D  G C A      G   +L +
Sbjct: 157 IFPLKDYSKQEIREIA-----LKIDLEVH------DKKDSQGVCFA----KEGYKEFLKE 201

Query: 217 K 217
            
Sbjct: 202 N 202


>UniRef50_C6MK22 Putative uncharacterized protein n=2 Tax=Deltaproteobacteria
           RepID=C6MK22_9DELT
          Length = 700

 Score = 64.8 bits (157), Expect = 2e-09,   Method: Composition-based stats.
 Identities = 49/242 (20%), Positives = 85/242 (35%), Gaps = 56/242 (23%)

Query: 5   VVVFSGGQDSTTCLV-QALQQYDEVHCVT------FDYGQRHRAEIDVARELALKLGARA 57
           VV+FSGG DS    + + L +   V  VT       +  +RHR  ++  + +A K G  A
Sbjct: 157 VVMFSGGLDSLAGAIDEVLNEKHRVVLVTHKSTPKLN--KRHRR-LE--KMIADKAGENA 211

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
              + V +     ++           YE         +V        ++ A  A  +  +
Sbjct: 212 PLHIGVRVNKNKGLN-----------YEYTQRSRSFLYV--------SIGATIAKMINLK 252

Query: 118 AVIT---GVCETDFSGYPDCR------------DEFVKALNHAVSLGMAKDIRFETPLMW 162
           +V     GV   +    P C                ++     +SL   +    E P +W
Sbjct: 253 SVRFYENGVISLNL---PVCAQVVGGRATRTTHPRVIRGFQEIISLVAGEPFTIENPYIW 309

Query: 163 IDKAETWALADYYGKLDLVRNETLTCYNGFK----GDGCGHCAACNLRANGLNHYLAD-- 216
             KA+   +    G  +++   T TC + ++       CG C+ C  R   +    AD  
Sbjct: 310 KTKADVVGVITKAGCQEMIAAST-TCTHTWEMTNHHTHCGTCSQCIDRRFAVIAAKADQH 368

Query: 217 KP 218
            P
Sbjct: 369 DP 370


>UniRef50_Q6WHP6 Putative uncharacterized protein n=1 Tax=Vibrio phage KVP40
           RepID=Q6WHP6_BPKV4
          Length = 228

 Score = 64.5 bits (156), Expect = 3e-09,   Method: Composition-based stats.
 Identities = 38/201 (18%), Positives = 69/201 (34%), Gaps = 32/201 (15%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           +++ S G DS   L + L++  + H V F +G           ++ +        ++++ 
Sbjct: 7   IILVS-GLDSLV-LYEFLER--KPHPVYFKWG-------FAIDDIEMAALPEETTIIEMP 55

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
            +  L                 E     N  + GRN L    +  +A++     +  G+ 
Sbjct: 56  EILHLKHDP------------YEFKTPRNPDLIGRNTLMFN-SVFHAFRP--NVIYRGML 100

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNE 184
           + D   Y D        +    S  + K+ R E P     K +   +A  YG        
Sbjct: 101 KGDDFYY-DASPTHSDEIAAMWSKMLNKECRIEQPFKDKTKRDIMQMAVEYGIDANSLQH 159

Query: 185 TLTCYNGFKGDGCGHCAACNL 205
              CYN  +   CG C+ C  
Sbjct: 160 ---CYN--REHNCGRCSKCVD 175


>UniRef50_Q3JDB4 Putative uncharacterized protein n=1 Tax=Nitrosococcus oceani ATCC
           19707 RepID=Q3JDB4_NITOC
          Length = 425

 Score = 62.9 bits (152), Expect = 9e-09,   Method: Composition-based stats.
 Identities = 37/240 (15%), Positives = 64/240 (26%), Gaps = 45/240 (18%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V + SGG DS    +  +        V+    Q    +     + A K+G   + +    
Sbjct: 141 VTLLSGGLDSFVGAIDLVADGKAPFAVS----QVAAGDKQSQADFAAKIGGGLNHL---- 192

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLA--AIYAYQVKAE----A 118
                        ++  P     +          R+ +FL     A  A +   +     
Sbjct: 193 ---------QLNHNVKCPGENERSQR-------ARSFIFLAYGVLAASAQKHYHDGERVD 236

Query: 119 VIT---GVCETDFSGYP------DCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKA 166
           +     G+   +    P        R     F+      + +     I    P  +  K 
Sbjct: 237 LYICENGLISINPPLTPARLGSLSTRTTHPVFLGLFQRLLDVA-ELRIAVRNPYQFRTKG 295

Query: 167 ETWALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           E          L     ET +C  Y       CG C  C +R    + +  D  T     
Sbjct: 296 EMLLECKDQAFLRKHAAETTSCGRYARNGWQHCGRCFPCLIRRAAFHAWGKDDRTKYVYA 355


>UniRef50_B2IBH3 tRNA-specific 2-thiouridylase mnmA n=141 Tax=root RepID=MNMA_BEII9
          Length = 431

 Score = 62.5 bits (151), Expect = 1e-08,   Method: Composition-based stats.
 Identities = 43/209 (20%), Positives = 77/209 (36%), Gaps = 43/209 (20%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVT---FDYGQR-HRA-------EIDVARELAL 51
           R VV  SGG DS+       ++  EV  VT   +D+G+  HR        +I  AR++A 
Sbjct: 31  RVVVAMSGGVDSSVVAALLKREGYEVIGVTLQLYDHGEAVHRKGACCAGQDIQDARQVAD 90

Query: 52  KLGARAHKVLDV------TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLT 105
           +LG   H VLD        +++  A S  T    P+P     ++              + 
Sbjct: 91  QLGI-PHYVLDYEERFRRKVIDPFAQSYAT-GETPIPCVSCNSE--------------IK 134

Query: 106 LAAIY--AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLG------MAKDIRFE 157
            A ++  A  + A+ + TG          D      ++L+ +              +   
Sbjct: 135 FADLFETAQHLGADVLATG-HYVSSKAEADGGRALYRSLDSSRDQSYFLFATTNAQLELL 193

Query: 158 -TPLMWIDKAETWALADYYGKLDLVRNET 185
             PL  + K +   +A   G +   + ++
Sbjct: 194 RFPLGDLAKPDVRQMARQLGLVVADKADS 222


>UniRef50_B0V938 Putative uncharacterized protein n=4 Tax=Acinetobacter baumannii
           RepID=B0V938_ACIBY
          Length = 442

 Score = 62.5 bits (151), Expect = 1e-08,   Method: Composition-based stats.
 Identities = 40/242 (16%), Positives = 70/242 (28%), Gaps = 43/242 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
            +V +FSGG DST   +    Q  +   V+  Y                    + H   D
Sbjct: 149 DSVCLFSGGLDSTIGAIDLKAQGKKSVLVSHAY--------------PKDRQKQDHVYYD 194

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA-------YQVK 115
           +   N             + +     +   +  +  R+  F+   A+ A       +  K
Sbjct: 195 LQFDNARFQ--------VLANPRKVKEIPADVQMRTRSFNFIAFGALIATALSKNHFSNK 246

Query: 116 AEAVIT---GVCETDFSGYP------DCR---DEFVKALNHAVSLGMAKDIRFETPLMWI 163
              +     G+   +    P        R     F++ LN      +   +  E P  + 
Sbjct: 247 IIKLYIPENGLISINPPLTPRRIGALSTRTTHPYFLQKLNILFK-NIGLPVEIENPYQYK 305

Query: 164 DKAETWALADYYGKLDLVRNETLTCYNGFK-GDGCGHCAACNLRANGLNHYLADKPTVMA 222
            K E          L  +   T++C    + G  CG C  C +R    + Y     T   
Sbjct: 306 TKGEMMLECKDQSSLKKIAATTVSCGKWKRSGMQCGKCLPCLIRRASFDKYQYKDLTQYQ 365

Query: 223 AM 224
             
Sbjct: 366 YS 367


>UniRef50_Q8PWQ6 Conserved protein n=3 Tax=Methanosarcina RepID=Q8PWQ6_METMA
          Length = 275

 Score = 62.1 bits (150), Expect = 1e-08,   Method: Composition-based stats.
 Identities = 37/172 (21%), Positives = 62/172 (36%), Gaps = 31/172 (18%)

Query: 4   AVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           AV+ FSGG DSTT    A ++  D    VT D     R +++ A + A ++G   HK+L 
Sbjct: 19  AVIAFSGGVDSTTLAALAFEELGDRALAVTIDSPLFPRHQLETASQAACEIGI-QHKILP 77

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
            +    L        +     Y  +                L     +A +    AV+ G
Sbjct: 78  FS----LNSVPYFTANTINRCYFCKKA-------------LLETLLEFAEKTGYNAVLEG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI--DKAETWALA 172
              ++  G    R  + +A+  A         +  +P +     K E   +A
Sbjct: 121 TNASEIKGE--NRPGY-RAIQEA-------GEKIYSPFVEFNVTKEEIREIA 162


>UniRef50_Q12C76 Putative uncharacterized protein n=1 Tax=Polaromonas sp. JS666
           RepID=Q12C76_POLSJ
          Length = 466

 Score = 61.8 bits (149), Expect = 2e-08,   Method: Composition-based stats.
 Identities = 39/235 (16%), Positives = 61/235 (25%), Gaps = 43/235 (18%)

Query: 9   SGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTLLNE 68
           S G DS     Q  +       +  +        +  A           H++ +   L  
Sbjct: 154 SNGLDSFALANQVQRDSSTTELILIN--------VQAA-----------HRLTNWKNLGR 194

Query: 69  LAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY------------QVKA 116
                +    + V   EP          P    +     A  A              +  
Sbjct: 195 ARDKDMQAVQVAVFFNEPHRTEPSFRSRPFIYDMLAGYGASLAQPAKVVIPENGQGSLGG 254

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW-ALADYY 175
             V  G    D + +  C   F   L   V+      +RFE P ++  K +    LA   
Sbjct: 255 SLVPLG----DEAPHRSCHPGFTSRLTRLVNALTNVTVRFEHPALFQTKGQVLAQLASQK 310

Query: 176 GKLDLVRNETLTC-Y------NGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
              +       +C Y         +   CG C  C LR   L     +  T   A
Sbjct: 311 SASEAWLAGHRSCSYDARHSSQDKRSMHCGVCGNCLLRRVALKWTGIEDTTDYRA 365


>UniRef50_A5INK8 ExsB family protein n=6 Tax=Thermotogaceae RepID=A5INK8_THEP1
          Length = 268

 Score = 61.8 bits (149), Expect = 2e-08,   Method: Composition-based stats.
 Identities = 42/189 (22%), Positives = 65/189 (34%), Gaps = 42/189 (22%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           K+ VV+FSGG DST       +   +    +T D     R EI+ A+ LA  +G R H+ 
Sbjct: 15  KKVVVMFSGGVDSTLLAKLTHEALGENSVALTIDSPVIPRKEIEKAKRLAGLIGIR-HEF 73

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYE-PEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +++  L        +R  I  P         + ++ V             +A +   E +
Sbjct: 74  IELNELK-------SRHLIENPPDRCYLCRKLRDSVVKN-----------WAKEHGFEVI 115

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI--DKAETWALADYYGK 177
             G+  +D   Y   R          V       I    P +     K E     D+  K
Sbjct: 116 ADGLNFSDLQDY---RPG--------VKASTEDGI--WHPFIEFEVTKEEI---RDFSRK 159

Query: 178 LDLVRNETL 186
           L L    T 
Sbjct: 160 LSLP---TW 165


>UniRef50_A8S2P7 Putative uncharacterized protein n=9 Tax=Firmicutes
           RepID=A8S2P7_9CLOT
          Length = 288

 Score = 61.4 bits (148), Expect = 2e-08,   Method: Composition-based stats.
 Identities = 47/179 (26%), Positives = 67/179 (37%), Gaps = 45/179 (25%)

Query: 8   FSGGQDSTTCLVQALQQ----YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           FSGG DS+  L  A         +V+ VTFD       ++++AR +A +LG   H+V+ V
Sbjct: 38  FSGGVDSSLLLKTAADAAAHTGRKVYAVTFDSRLHPSCDLEIARRVAGELGGI-HEVITV 96

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL----FLTLAAIYAYQVKAEAV 119
                                E E + I N  V  R  L      +  A  A       +
Sbjct: 97  D--------------------ELEQESIKNNPV-NRCYLCKRHLFSRLAELAEARGIRYI 135

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL--MWIDKAETWALADYYG 176
           + G  E D   Y   R   ++AL               +PL  + I KA+  ALA  YG
Sbjct: 136 LDGTNEDDMHVY---RPG-IRALKEL---------GIISPLAELHITKAQVKALASEYG 181


>UniRef50_D0XY41 Putative uncharacterized protein n=1 Tax=Caulobacter segnis ATCC
           21756 RepID=D0XY41_9CAUL
          Length = 458

 Score = 61.4 bits (148), Expect = 2e-08,   Method: Composition-based stats.
 Identities = 33/134 (24%), Positives = 47/134 (35%), Gaps = 17/134 (12%)

Query: 101 ILFLTLAAIYAYQVKAEAVITG------VCETDFSGYPDCR--DEFVKALNHAVSLGMAK 152
            +   +AA+ A   K     +G      V       YPD R    F   +    +  +  
Sbjct: 218 AVITGIAAVLAKADKIVVTESGQGALGPVLTVSGQAYPDYRVHPAFTMRVERLFATLLGH 277

Query: 153 DIRFETPLMWIDKAETWALADYYGKLDLVRNETLTCYN-------GFKGDGCGHCAACNL 205
              ++ P +W  K ET A A           ET +C+        G K   CG CAAC L
Sbjct: 278 SAHYDFPRLWSTKGETIAEAARATPQ--PWAETRSCWQQSRQVGMGGKQRQCGVCAACML 335

Query: 206 RANGLNHYLADKPT 219
           R   L+     +P 
Sbjct: 336 RRMSLHAAGVVEPA 349


>UniRef50_D0LKW7 Queuosine synthesis-like protein n=1 Tax=Haliangium ochraceum DSM
           14365 RepID=D0LKW7_HALO1
          Length = 296

 Score = 61.0 bits (147), Expect = 3e-08,   Method: Composition-based stats.
 Identities = 41/176 (23%), Positives = 60/176 (34%), Gaps = 30/176 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLG-ARAHKV 60
           R +V FSGG DS   L  A  +   + H +T       R+E++ AR L  +LG A  H V
Sbjct: 32  RVLVAFSGGVDSAFLLKIASDELGADCHALTCVSVTMARSEVEDARALGDELGLAERHHV 91

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++   L +   +        +   E      P                  A Q+  E + 
Sbjct: 92  VESNELEQPGFADNPTHRCALCKTELMDVARP-----------------LAEQLGVEVIA 134

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            G    D     D R     A   A  + M      E     + KA+  AL+   G
Sbjct: 135 IGTNIDDLG---DFRPGIAAAKERAARMPM-----VEA---GLSKADIRALSRQLG 179


>UniRef50_C6J374 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Bacillales RepID=C6J374_9BACL
          Length = 410

 Score = 61.0 bits (147), Expect = 3e-08,   Method: Composition-based stats.
 Identities = 42/190 (22%), Positives = 71/190 (37%), Gaps = 33/190 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYG--QR--HRAEIDVARELALKLGARA 57
           +A+++ SGG DS      AL++  E+ CV F  Y    +   +  ID+A  LA   G   
Sbjct: 180 KAMLLLSGGIDSPVAGWSALRRGLEIECVHFHSYPFTSKKAEQKVIDLANVLAQYSGFIK 239

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             ++  T              I     +   D +  T    R    L +A   A +  A 
Sbjct: 240 LHLVPFT-------------EIQTKLAQTGQDNLIITM--MRRA-MLRIATKLAERNGAL 283

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A++TG      +          + L+   ++G   ++    PL+  DK +  A+A   G 
Sbjct: 284 ALVTGDSLGQVAS---------QTLSSMNAIGRVTNLPILRPLVMTDKVDIIAMAQQIGT 334

Query: 178 LD---LVRNE 184
            D   L   +
Sbjct: 335 YDLSILPYED 344


>UniRef50_Q5JCY1 Probable tRNA sulfurtransferase n=14 Tax=Thermococcaceae
           RepID=THII_PYRKO
          Length = 381

 Score = 61.0 bits (147), Expect = 3e-08,   Method: Composition-based stats.
 Identities = 28/183 (15%), Positives = 60/183 (32%), Gaps = 29/183 (15%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELAL-----KLGARA 57
           + V + SGG DS       +++  EV  V    G      ++  R++         G +A
Sbjct: 182 KVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMG---EKTLEKVRKIWNQLKKYHYGGKA 238

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             ++    +       + +    +   +                + +  A   A +  A+
Sbjct: 239 ELIV----VKPQNREEMLKKIKELGKEKYTCVLCK--------FMMVKHADRIAKEFGAK 286

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
            ++ G      +          + L +   +  A D+    PL+ +DK E   +A   G 
Sbjct: 287 GIVMGDSLGQVAS---------QTLENMYIVSQASDLPIYRPLIGLDKEEIVDIAKKIGT 337

Query: 178 LDL 180
            +L
Sbjct: 338 FEL 340


>UniRef50_B1M991 Putative uncharacterized protein n=2 Tax=Proteobacteria
           RepID=B1M991_METRJ
          Length = 435

 Score = 61.0 bits (147), Expect = 3e-08,   Method: Composition-based stats.
 Identities = 41/232 (17%), Positives = 72/232 (31%), Gaps = 30/232 (12%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVT------FDYGQRHRAEIDVARELALKLGARA 57
           +  +FSGG DS    +  + Q +    V+        Y  R    + VA     +  A A
Sbjct: 150 SACLFSGGLDSYIGALDLIAQGERPLLVSHAYRGDRRYQDRTAHALPVA---CARFSANA 206

Query: 58  HKV----LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT-FVPGRNILFLTLAAIYAY 112
           H       DV+ +   + + L   +           G   T +VP  N   + L A    
Sbjct: 207 HPTWSGPSDVS-MRTRSFNFLAYGAAAATAISQMRAGNRVTLWVPE-NG-LIALNAPLTP 263

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
           +        G   T  +        F++++ + +   +   +    PL    K E     
Sbjct: 264 RR------IGSHSTRTTH-----PYFLQSMQNILD-AVGLPVAIVNPLRHQTKGEMVLRH 311

Query: 173 DYYGKLDLVRNETLTCYNGFK-GDGCGHCAACNLRANGLNHYLADKPTVMAA 223
                       T++C    + G  CG C  C +R    +       T   +
Sbjct: 312 ARDPAFVAQAVRTVSCGKWKRDGTQCGRCVPCLIRRASFHAADIADTTGYRS 363


>UniRef50_A0PSW2 Putative uncharacterized protein n=1 Tax=Mycobacterium ulcerans
           Agy99 RepID=A0PSW2_MYCUA
          Length = 420

 Score = 60.6 bits (146), Expect = 4e-08,   Method: Composition-based stats.
 Identities = 32/144 (22%), Positives = 41/144 (28%), Gaps = 22/144 (15%)

Query: 99  RNILFLTLAAIYAYQVKAEAV------ITGVC---ETDFSGYPDCR---DEFVKALNHAV 146
           R +LF  LA   A     + V       TG+      D  G    R      +  +   +
Sbjct: 207 RALLFYALAVALADARGIDRVEVSENGFTGLNLSLGNDRGGVLSTRSTHPWTMHLMQRLL 266

Query: 147 SLGMAKDIRFETPLMWIDKAETWALA-DYYGKLDLVRNETLTC-------YNGFK-GDGC 197
                 DI    P  W  K E    A D           TL+C       Y G      C
Sbjct: 267 DDA-GIDIELVNPYEWQTKGELVRAAADVCPAFAEGLVTTLSCAKLDGRTYKGGNPNLNC 325

Query: 198 GHCAACNLRANGLNHYLADKPTVM 221
           G C AC  R   +     +  T  
Sbjct: 326 GLCVACLTRRASIRAAGLEDKTPY 349


>UniRef50_A0YNH9 Putative uncharacterized protein n=2 Tax=Oscillatoriales
           RepID=A0YNH9_9CYAN
          Length = 488

 Score = 60.6 bits (146), Expect = 4e-08,   Method: Composition-based stats.
 Identities = 45/239 (18%), Positives = 78/239 (32%), Gaps = 44/239 (18%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARAHKV- 60
           V ++SGG D+   L   LQ    V  + F  G     +  +  V + +        +   
Sbjct: 158 VALWSGGLDALAGLYTRLQTSHTVPFMLFGTGSSKSVYARQEKVFQAIIKSFPTHLYLCR 217

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  LN    S L R +                    R ++F  L A  AY +    + 
Sbjct: 218 VPINFLN----SKLLRKNKITR---------------ARGVVFTLLGAACAYLMGQRTLS 258

Query: 121 -----TGV---------CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKA 166
                 G             D S         +  ++  VS  + ++ R   P +++ KA
Sbjct: 259 VYENGIGAINLPYRASAVGLDHSR--SVHPLTLLMVSDVVSELLGENFRVYNPFLFLTKA 316

Query: 167 ETWALADYYGKLDLVRNETLTC----YNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           +         + DLV   T +C        +   CG+C++C LR   L     +  T  
Sbjct: 317 QICRALAENQENDLV-AITQSCDSRHRKSQQPTQCGYCSSCILRKQALAASKIEDKTRY 374


>UniRef50_Q5WAJ2 Putative uncharacterized protein n=4 Tax=Bacillus
           RepID=Q5WAJ2_BACSK
          Length = 430

 Score = 60.2 bits (145), Expect = 5e-08,   Method: Composition-based stats.
 Identities = 37/241 (15%), Positives = 75/241 (31%), Gaps = 41/241 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHKVL 61
           + V +FSGG DS    ++ L    E   +    YG      I   +++  +     +++ 
Sbjct: 145 KKVCMFSGGLDSFIGAIELLDDTSENDTLFVSHYG--GGKGIKEYQDILREELISKYQLT 202

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK--AEAV 119
           +    +  A              +   +    T    R+ +F + A + A  V      V
Sbjct: 203 ESNFYSFYA------------SAKNGVEDTTRT----RSFMFFSHAVVLASSVDRPVNLV 246

Query: 120 IT--GVCETDFS------GYPDCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKAET 168
           I   G+   +        G    R     ++      +   +   ++   P  +  K E 
Sbjct: 247 IPENGLISLNIPLTNSRLGSSSTRTTHPFYLSLFQKLLD-NLKIMVKILNPYQFKTKGEM 305

Query: 169 WALADYYGKLDLVRNETLTCYNGFKGD--------GCGHCAACNLRANGLNHYLADKPTV 220
                    L      T++C +   G          CG+C  C +R + + +      T+
Sbjct: 306 IRECSSIEFLQKNITNTMSCSHPDWGRWAGESEVCHCGYCLPCVIRRSAIKNAGIQDETI 365

Query: 221 M 221
            
Sbjct: 366 Y 366


>UniRef50_C6BXJ5 ExsB family protein n=1 Tax=Desulfovibrio salexigens DSM 2638
           RepID=C6BXJ5_DESAD
          Length = 269

 Score = 60.2 bits (145), Expect = 6e-08,   Method: Composition-based stats.
 Identities = 40/189 (21%), Positives = 67/189 (35%), Gaps = 48/189 (25%)

Query: 4   AVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFD--Y-GQRHRAEIDVARELALKLGARAHK 59
           AV+ FSGG DST  L  A +   D+    +    Y  Q  + E   A+E A ++  + H 
Sbjct: 21  AVIAFSGGVDSTLLLHTAKEALGDKAIAASISTPYVPQWEQGE---AKEFAEQMEVK-HV 76

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V+D+    EL ++         P++                 +  +     A +   E V
Sbjct: 77  VVDMGFPEELRMNP--------PEHCYTCKN-----------ILFSKLLEIAQENDIEHV 117

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLM--WIDKAETWALADYYGK 177
           + G    D S Y   R   +KAL               +P +   + K E   ++    +
Sbjct: 118 LEGTNIDDLSDY---RPG-IKALREL---------EIRSPFVEAELTKQEIRDISR---R 161

Query: 178 LDLVRNETL 186
            +L    T 
Sbjct: 162 FNLP---TW 167


>UniRef50_A2BKB5 Probable thiamine biosynthesis protein, ThiI n=1 Tax=Hyperthermus
           butylicus DSM 5456 RepID=A2BKB5_HYPBU
          Length = 388

 Score = 59.8 bits (144), Expect = 8e-08,   Method: Composition-based stats.
 Identities = 37/185 (20%), Positives = 59/185 (31%), Gaps = 40/185 (21%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RA+V+FSGG DS        ++  EV  + F         I      A        ++ +
Sbjct: 181 RALVLFSGGLDSPVAAWYTAKRGVEVDLLHF---------ILAVPASARDAERVGRRLAE 231

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNI------LFLTLAAIY-----A 111
           + L        L                 P T     N       + L LA  Y     A
Sbjct: 232 LWLYGYKPKLFLVD-------------FRPVTVTIASNTRRGYEQIVLRLAMYYAAQLIA 278

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
            +   +AV+TG      S       + +K +  A++  +   +    PL  +DK E   +
Sbjct: 279 EKHGYDAVVTGESIGQVSS------QTLKNM-KAMAQALPLRLPLIRPLAGMDKEEIVTV 331

Query: 172 ADYYG 176
               G
Sbjct: 332 CRRIG 336


>UniRef50_B6YWN1 ThiI-related protein n=5 Tax=Euryarchaeota RepID=B6YWN1_THEON
          Length = 196

 Score = 59.5 bits (143), Expect = 8e-08,   Method: Composition-based stats.
 Identities = 36/183 (19%), Positives = 67/183 (36%), Gaps = 24/183 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVAR--ELALKLGARAHKV 60
           +AV + S G DS   +   L++  E+  V F   Q  + E  V    E+  K G     V
Sbjct: 2   KAVALLSSGIDSPVAIYLMLRKGMEITPVHFK--QSDKKESKVLELCEILSKYGKLNEPV 59

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + V    E   + +    + +   +        T         L  A    +++ A+A++
Sbjct: 60  I-VDAYEE--QAPVFSKLVEIGKAKWTCLFCKYT--------MLRKACRVGHEIGAKAIV 108

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           TG      +          + L++ + +  A D+    PL+  DK E   +A   G  ++
Sbjct: 109 TGDSLGQVAS---------QTLDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEI 159

Query: 181 VRN 183
              
Sbjct: 160 SIE 162


>UniRef50_B9L3Q7 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Thermomicrobia (class) RepID=B9L3Q7_THERP
          Length = 407

 Score = 59.5 bits (143), Expect = 9e-08,   Method: Composition-based stats.
 Identities = 40/178 (22%), Positives = 68/178 (38%), Gaps = 27/178 (15%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGA---RAH 58
           R V + SGG DS     + +++   V  V F  Y       I+ A ELA  L       +
Sbjct: 182 RVVALLSGGFDSPVAAYRLMKRGCFVTFVHFHAYPYVRPISIEKAVELARHLSRFQPPKN 241

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +++ V + +   V +L             A+      +  R  L L +A   A++  A A
Sbjct: 242 RLIVVPIGDAQRVIAL------------AAEPSLRVVLYRR--LMLRIATAIAHEEGAGA 287

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
           ++TG      +          + L +  ++G   D+    PL+  +K E  A A   G
Sbjct: 288 LVTGESLGQVAS---------QTLENMRAIGAVTDLPLLRPLVGNNKDEIIAEAVRIG 336


>UniRef50_Q3SW10 Putative uncharacterized protein n=1 Tax=Nitrobacter winogradskyi
           Nb-255 RepID=Q3SW10_NITWN
          Length = 452

 Score = 59.5 bits (143), Expect = 9e-08,   Method: Composition-based stats.
 Identities = 23/96 (23%), Positives = 37/96 (38%), Gaps = 11/96 (11%)

Query: 131 YPD--CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNETLTC 188
           YPD      F   +   ++    + +RF  P +W  KAET    +Y        ++T +C
Sbjct: 249 YPDYRNHPLFTSRMERFLNALFRRPVRFVFPRLWHTKAETLR--EYAALPGSKWDDTKSC 306

Query: 189 YNGFKGDG-------CGHCAACNLRANGLNHYLADK 217
           +   +          CG CAAC LR   ++      
Sbjct: 307 WRNARWSALNGNLVQCGICAACMLRRMSIHTAGLVD 342


>UniRef50_A8AM74 Putative uncharacterized protein n=8 Tax=Gammaproteobacteria
           RepID=A8AM74_CITK8
          Length = 446

 Score = 59.5 bits (143), Expect = 9e-08,   Method: Composition-based stats.
 Identities = 41/242 (16%), Positives = 77/242 (31%), Gaps = 53/242 (21%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDY-GQRHRAEIDVARELALKLGARAHKVLDV 63
           V +FSGG DS   ++  L    +   ++  Y G + +                AHK+ + 
Sbjct: 157 VSLFSGGLDSAIGVIDFLSSGRKPLLISHSYKGDKTKQ------------DQIAHKIREK 204

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
              + L  S+      P+         +       R++ FL  A + AY VK        
Sbjct: 205 GTFSRLRSSA-----NPIGRGMTRDITMRT-----RSLNFLAFALVGAYAVKQIN----- 249

Query: 124 CETDFSGY-PDC----------------------RDEFVKALNHAVSLGMAKDIRFETPL 160
              D S + P+                          F+  +    +  +   +R   P 
Sbjct: 250 NHKDLSIFVPENGFISLNAPLTHRRVGSLSTRTTHPYFIGMIQELFN-NLGFGVRLINPY 308

Query: 161 MWIDKAETWALADYYGKLDLVRNETLTC-YNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
            ++ K +  +       L  + + T++C +   K   CG+C  C +R   L      +  
Sbjct: 309 QFMTKGQMVSNCKDSKMLSEIVDLTVSCSHWKRKNQQCGYCVPCMIRRAALMKGTLKESI 368

Query: 220 VM 221
             
Sbjct: 369 SY 370


>UniRef50_Q0C5H6 Putative uncharacterized protein n=1 Tax=Hyphomonas neptunium ATCC
           15444 RepID=Q0C5H6_HYPNA
          Length = 456

 Score = 59.5 bits (143), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 40/135 (29%), Positives = 48/135 (35%), Gaps = 19/135 (14%)

Query: 99  RNILFLTLAAIYA-YQVKAEAVITGVCETDFSG--------YPDCR--DEFVKALNHAVS 147
           R  LF  +AA  A     AE V+    +  F          +PD R    F  AL   VS
Sbjct: 215 RGFLFGAVAATAAGIMKGAEIVVPESGQGTFGPALTVIGHEHPDVRMSPIFTSALGRFVS 274

Query: 148 LGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNETLTC-------YNGFKGDGCGHC 200
                 IRF  P +W  K ET A A    +L      T++C         G     CG C
Sbjct: 275 RIFESPIRFVHPRVWSTKGETLA-ALQIAQLSEGWEVTVSCSRSSRRKIQGSVAKHCGVC 333

Query: 201 AACNLRANGLNHYLA 215
           A C LR   L     
Sbjct: 334 ANCLLRRQSLQVAGL 348


>UniRef50_Q9ZJQ0 tRNA-specific 2-thiouridylase mnmA n=22 Tax=Campylobacterales
           RepID=MNMA_HELPJ
          Length = 342

 Score = 59.1 bits (142), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 46/195 (23%), Positives = 70/195 (35%), Gaps = 40/195 (20%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE------IDVARELALKLGAR 56
           +  V+ SGG DS+       +Q  E+  +   Y + H +E      I  A++    LG  
Sbjct: 2   KIAVLLSGGVDSSYSAYSLKEQGHELVGI---YLKLHASEKKHDLYIKNAQKACEFLGI- 57

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGR--NILF-LTLAAIYAYQ 113
             +VLD     +   S++  + I       E    PN   P    N L    LA  +A +
Sbjct: 58  PLEVLD---FQKDFKSAVYDEFINA----YEEGQTPN---PCALCNPLMKFGLALDHALK 107

Query: 114 VKAEAVITG----VCETDFSGY----PDCRDE---FVKALNHAVSLGMAKDIRFETPLMW 162
           +  E + TG    V E D   Y     D   +   F+ AL H          +   PL  
Sbjct: 108 LGCEKIATGHYARVKEIDKVSYIQEALDKTKDQSYFLYALEH------EVIAKLVFPLGD 161

Query: 163 IDKAETWALADYYGK 177
           + K +   LA     
Sbjct: 162 LLKKDIKPLALNAMP 176


>UniRef50_Q8TWX3 ATP pyrophosphatase involved in thiamine biosynthesis n=1
           Tax=Methanopyrus kandleri RepID=Q8TWX3_METKA
          Length = 374

 Score = 59.1 bits (142), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 37/186 (19%), Positives = 62/186 (33%), Gaps = 27/186 (14%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---YGQRHRAEIDVARELALKLGARAHK 59
           R V + SGG DS      A ++  EV  +  D   YG     E+D    LA K       
Sbjct: 167 RVVCLVSGGIDSPVAAWYAARRGCEVIWLHLDRGKYGS----EVDAVERLAGKFSEWLPS 222

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
            +++ + +         D +   +            +  R ++    A      V A AV
Sbjct: 223 DVELLIED-------FEDFMESLEGLEGESARYRCVLCKREMI--RRACDVCENVGAVAV 273

Query: 120 ITG-VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           + G V     S  PD     +  ++               PL+  DK E   L++  G  
Sbjct: 274 VMGDVIGQVASQIPDN----LAVIDRV------ARFPVFRPLLGFDKNEVQRLSERLGLF 323

Query: 179 DLVRNE 184
           ++ +  
Sbjct: 324 EVSKEH 329


>UniRef50_B9QRC5 Putative uncharacterized protein n=1 Tax=Labrenzia alexandrii
           DFL-11 RepID=B9QRC5_9RHOB
          Length = 457

 Score = 59.1 bits (142), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 30/131 (22%), Positives = 46/131 (35%), Gaps = 19/131 (14%)

Query: 103 FLTLAAIYAYQVKAEAVIT-----GVCETDFSG----YPD--CRDEFVKALNHAVSLGMA 151
           F  ++ I AY   A  +I      G      +     YPD      F + +   +     
Sbjct: 216 FALISGIAAYLSGASEIIIPESGQGAIGPAIAPVVHAYPDYRNHPRFTQRMERLLKALFG 275

Query: 152 KDIRFETPLMWIDKAETWALADYYGKLDLVRNETLTCYN-------GFKGDGCGHCAACN 204
           K+IR+  P +W  K ET   +          + T +C+          K   CG CAAC 
Sbjct: 276 KEIRYVFPRLWNTKGETLR-SFATLPEGSNWHTTRSCWRSNRWCSVNGKRRQCGVCAACM 334

Query: 205 LRANGLNHYLA 215
           LR   ++    
Sbjct: 335 LRRLSVHAAGL 345


>UniRef50_A6LQW7 ExsB family protein n=4 Tax=Clostridium RepID=A6LQW7_CLOB8
          Length = 274

 Score = 58.7 bits (141), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 45/179 (25%), Positives = 68/179 (37%), Gaps = 40/179 (22%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFD--YGQRHRAEIDVARELALKLGARAHK 59
           + V+ FSGG DST  L  A +   D V  VT    Y  +   EI  AREL  +LG   H+
Sbjct: 19  KVVLAFSGGVDSTFLLRVAKEALGDNVKAVTILSPYIPKW--EIAEARELVRELGV-QHE 75

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +++  +++ +  +              +   +  T V        T+    A +     V
Sbjct: 76  IIEAPIIDSIKFNP------------EDRCYLCKTAV-------FTMILNVAKEQGYNCV 116

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMW--IDKAETWALADYYG 176
           I G    D S Y   R   +KAL               +PL+   + KAE  A +   G
Sbjct: 117 IDGTNFDDISDY---RPG-LKALKEL---------EVRSPLLECKLTKAEIRAFSKELG 162


>UniRef50_Q13XN6 Putative uncharacterized protein n=1 Tax=Burkholderia xenovorans
           LB400 RepID=Q13XN6_BURXL
          Length = 458

 Score = 58.7 bits (141), Expect = 1e-07,   Method: Composition-based stats.
 Identities = 45/242 (18%), Positives = 68/242 (28%), Gaps = 50/242 (20%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K   V +S G DS      +  +  E  C+                    K G      +
Sbjct: 146 KTFAVAYSNGLDSRAVSALSGDEG-EALCIRV-----------AGNRQPRKDGDSYFTHI 193

Query: 62  DVTLLNELAVSSLTRDSI----PVPDYEPEADGIPNTFVP-----GRNILFLTLAAIYAY 112
              +       S  R        V     +   +    VP         + L L  +YA 
Sbjct: 194 PFNISGYRGNESSFRSRGFQFAAVTAIAAQLSNVTRIVVPESGQGALGPVLLPLYGVYA- 252

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW-AL 171
                         D+  YP     F + +   +       +RFE P +W  K +T  A 
Sbjct: 253 --------------DYRNYP----TFFRKMERFIKALQGYQVRFEQPRLWFTKGQTLSAF 294

Query: 172 ADYYGKLDLVRNETLTCYNG------FKGDGCGHCAACNLRANGLNHYLA---DKPTVMA 222
               GK       T +C+             CG CAAC LR   L+           V++
Sbjct: 295 LRIPGKSGEHLKSTRSCWQTRRVVNVGSRKQCGLCAACLLRRLSLHAAGVSEAPDTYVVS 354

Query: 223 AM 224
            +
Sbjct: 355 DL 356


>UniRef50_C9YE00 Putative uncharacterized protein (Fragment) n=2 Tax=Proteobacteria
           RepID=C9YE00_9BURK
          Length = 82

 Score = 58.7 bits (141), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 30/80 (37%), Positives = 35/80 (43%), Gaps = 19/80 (23%)

Query: 162 WIDKAETWALADYYGK-----------LDLVRNETLTCYNGFK------GDGCGHCAACN 204
           WI+KA+TW LA   G+           +DLV   T TCY G +      G GCG C AC 
Sbjct: 5   WINKAQTWELAHELGERSGVPEGGQALVDLVVEHTHTCYLGDRTHRHAWGYGCGTCPACE 64

Query: 205 LRANGLNHYLADKPTVMAAM 224
           LRA G            AA 
Sbjct: 65  LRAKGY--AGFVDKVNKAAA 82


>UniRef50_O29382 Probable tRNA sulfurtransferase n=2 Tax=Archaeoglobaceae
           RepID=THII_ARCFU
          Length = 374

 Score = 58.7 bits (141), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 39/187 (20%), Positives = 68/187 (36%), Gaps = 27/187 (14%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ--RHRAEIDVARELALKLGARAHKV 60
           + V + SGG DS      A+++  EV  V F   Q        +  R LA KL     K+
Sbjct: 167 KVVALISGGIDSPVAAFMAMKRGCEVVAVHFF-NQTMHSPKVREKIRMLAEKLAEYQGKI 225

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                  +L +         + +  P    +    V  R  +   +A + A +   +AV+
Sbjct: 226 -------KLYMVPFKEVQFEIINRVPPKLRM----VVYRRSMM-RMANLIAEKEGCKAVV 273

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD- 179
           TG   +  +       + +  +N   S   A  +    PL+ +DK E  A+A      + 
Sbjct: 274 TGDSLSQVAS------QTLDNINVIYS---ASKLAVLPPLIGMDKEEIVAIAKRIETYEI 324

Query: 180 --LVRNE 184
             L   +
Sbjct: 325 SILPYED 331


>UniRef50_B5YCZ9 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=2
           Tax=Dictyoglomus RepID=B5YCZ9_DICT6
          Length = 345

 Score = 58.3 bits (140), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 40/190 (21%), Positives = 67/190 (35%), Gaps = 35/190 (18%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD--YGQRHRAEIDVARELALKLGARAHKV 60
           +  V  SGG DS+T      ++  EV  +T +  Y       I  A ++A  L    H  
Sbjct: 4   KVAVGMSGGVDSSTTAYLLKKEGYEVIGLTMNIAY----SDSIKDAEKIAKYLNI-PHFY 58

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP------GRNILFLTLAAIYAYQV 114
           +D+            R        +   D   + + P       R I+   L    A ++
Sbjct: 59  VDL------------RKEFEEQIIKYFIDSYKSGYTPNPCTFCNR-IIKFGLLLKKAQEL 105

Query: 115 KAEAVITG-----VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE---TPLMWIDKA 166
            AE + TG     V + +   +   R + +K  + +  L      + E    PL  + K 
Sbjct: 106 GAEYLATGHYVRKVWDEEKKVFLLKRAKDLKK-DQSYFLAYLTQEQIEKSLFPLGDLTKN 164

Query: 167 ETWALADYYG 176
           E   LA+  G
Sbjct: 165 EVRKLAEEIG 174


>UniRef50_A4T1U6 Putative uncharacterized protein n=2 Tax=Actinomycetales
           RepID=A4T1U6_MYCGI
          Length = 454

 Score = 58.3 bits (140), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 53/243 (21%), Positives = 90/243 (37%), Gaps = 36/243 (14%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDE--VHCVTFDYG-QRHRAEIDVARELALKLGARAHKV 60
           AV +FSGG DS T ++  L++  +  +  ++ + G Q   A+  +   L    G      
Sbjct: 144 AVSLFSGGLDSLTGVIDLLEEDPDRRLCLISHNEGDQASTAQKTLTAALREHYG---QDR 200

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA-V 119
           L++  L      ++   + P+P           T    R++LFLT     A  V  +  V
Sbjct: 201 LELRSLFLRPAPAVAGQARPLPPERE-------TTTRARSLLFLTAGLALAASVGPDVPV 253

Query: 120 IT---GVCETDF------SGYPDCR---DEFVKALNHAV-SLGMAKDIRFETPLMWIDKA 166
                G    +       SG    R    +F++ L  A  ++G+   I    P     K 
Sbjct: 254 YIPENGFIGINVPLTRARSGSYSTRTTHPQFMQLLRTAADAVGVCNPI--LNPFRLKTKG 311

Query: 167 ETWALADYYGKLDLVRNETLTC-------YNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
           E  A +     L  +   +++C       Y   K   CG+C  C +R + +     D P 
Sbjct: 312 EILASSRNPELLRSLAPNSISCSHPEAARYAVRKQGNCGYCFPCLIRRSSMARVGWDSPE 371

Query: 220 VMA 222
             A
Sbjct: 372 GYA 374


>UniRef50_Q72HP1 Probable tRNA sulfurtransferase n=3 Tax=Thermus RepID=THII_THET2
          Length = 406

 Score = 58.3 bits (140), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 42/180 (23%), Positives = 66/180 (36%), Gaps = 32/180 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR------AEIDVARELALKLGAR 56
           R V + SGG DS     + +++  EV  V F     H       A  + A+ LA +L   
Sbjct: 176 RVVALLSGGIDSPVAAYRLMRRGAEVVLVHF-----HPFPLLSGASREKAKALAERLARF 230

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H++     L+ +  S + R  I       EA       +  R    L +A   A +  A
Sbjct: 231 QHRL----RLHLVPFSEVQRHII------VEAPTAYRVVLYRR--YMLRIAEAIAREEGA 278

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A+ TG      +          + L +  ++  A  +    PL+  DK E  A A   G
Sbjct: 279 LALCTGDSLGQVAS---------QTLENLHAVNQAATLPVFRPLIGWDKEEIVAEAQRIG 329


>UniRef50_Q5P5F5 Putative uncharacterized protein n=1 Tax=Aromatoleum aromaticum
           EbN1 RepID=Q5P5F5_AZOSE
          Length = 457

 Score = 57.9 bits (139), Expect = 2e-07,   Method: Composition-based stats.
 Identities = 24/111 (21%), Positives = 39/111 (35%), Gaps = 12/111 (10%)

Query: 130 GYPD--CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNETLT 187
           GY D      F   +    +  +   I++  P +W+ K ET          +     T +
Sbjct: 250 GYEDYRNHPVFTALMARFFNALLGYKIQYRFPRLWMTKGETLREFVDVCGSEARWATTRS 309

Query: 188 CYN-------GFKGDGCGHCAACNLRANGLNHYL---ADKPTVMAAMKQKT 228
           C+          +   CG CAAC LR   ++        K  V  ++K  T
Sbjct: 310 CWQQSRQVAVSGERRQCGVCAACILRRLSVHAAGLNELPKTYVWESLKAAT 360


>UniRef50_Q65G52 Probable tRNA sulfurtransferase n=20 Tax=Bacillales
           RepID=THII_BACLD
          Length = 401

 Score = 57.9 bits (139), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 34/178 (19%), Positives = 59/178 (33%), Gaps = 30/178 (16%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---YGQRHRAE--IDVARELALKLGARA 57
           +A+++ SGG DS      A+++  E+  V F    Y      +  ID+ + L    G   
Sbjct: 179 KAMLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIK 238

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             ++             T+    +    PE   +  T    R  L L +A     +  A 
Sbjct: 239 LHIVPF-----------TKTQELIQKQIPENYSMTAT----R-RLMLQIADKLRERHNAL 282

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
           A+ TG      +          + L    ++          PL+ +DK E    A   
Sbjct: 283 AIFTGESLGQVAS---------QTLESMYAINAVTSTPVLRPLIGMDKTEIIEKAKEI 331


>UniRef50_Q9Y933 GMP synthase [glutamine-hydrolyzing] n=5 Tax=Archaea
           RepID=GUAA_AERPE
          Length = 512

 Score = 57.9 bits (139), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 42/191 (21%), Positives = 69/191 (36%), Gaps = 33/191 (17%)

Query: 4   AVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           AV   SGG DST   + A +     ++ V  D+G     E++   +L  +LG     V  
Sbjct: 221 AVAAVSGGVDSTVAALIAKKAIGSRLYPVFIDHGLHPEGEVERVVKLLSRLGLEPVMV-- 278

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                E  +++L     P               V GR  ++  +    A  + AE ++ G
Sbjct: 279 --DAGEEFLAALEGVGDPEEKRR----------VVGR--VYAEVLERAARDIGAEYLVQG 324

Query: 123 VCETDFSGYPD-----CRD--EFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
                   YPD      R   + +K  ++   L     ++   PL +  K E   LA+  
Sbjct: 325 TI------YPDVIESGARPGADTIKTHHNVGGLPKDMRLKLVEPLRYFYKDEVRLLAE-- 376

Query: 176 GKLDLVRNETL 186
            KL + R    
Sbjct: 377 -KLGVPRELIW 386


>UniRef50_C5A5U2 Predicted ATPase of the PP-loop superfamily n=8 Tax=Thermococcaceae
           RepID=C5A5U2_THEGJ
          Length = 262

 Score = 57.9 bits (139), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 36/176 (20%), Positives = 56/176 (31%), Gaps = 27/176 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG-QRHRAEIDVARELALKLGARAHKV 60
           KR +V+FSGG+DS+  L    +   +V  +TF +    H   +  A +   KLG   H +
Sbjct: 21  KRILVLFSGGKDSSLTLYLLKEAGLDVSALTFFHRWSWHET-LRWAMKFTRKLGVE-HYL 78

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+           T   +           I    V       L  A  +A     E + 
Sbjct: 79  VDI-----------TDGLLREAVGRKGPVCIHCKRV------MLWNAKWFALNNGFEILA 121

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETWALADY 174
            G    D           +      + L     I      PL+     E   LA+ 
Sbjct: 122 KGDNANDKII-----GALLDQCKGDIRLCRIPRIGLPILRPLIKYTAEEVERLAEE 172


>UniRef50_A3DLP8 Thiamine biosynthesis protein n=1 Tax=Staphylothermus marinus F1
           RepID=A3DLP8_STAMF
          Length = 375

 Score = 57.5 bits (138), Expect = 3e-07,   Method: Composition-based stats.
 Identities = 33/178 (18%), Positives = 57/178 (32%), Gaps = 31/178 (17%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           VV+ SGG DS+     A+++   +  V  D G ++ +E    R     L      V    
Sbjct: 168 VVLLSGGVDSSLAAWYAMKRGARIIPVFIDLG-KYWSE-QARRRFYEALSLVYEWV---P 222

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGR------NILFLTLAAIYAYQVKAEA 118
                       D I           + +T +P R            +A+I A + +   
Sbjct: 223 W-----------DKIKTYIVRGAEKILESTNIPARLRCLLCKANMYRIASIIADKEECLG 271

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
           ++TG      +          + L +   L          P+ ++DK E    A   G
Sbjct: 272 IVTGEAVGQVAS---------QTLRNLYILSSISPKPIYRPVAFMDKLEIIEQARKLG 320


>UniRef50_A6KXY6 Argininosuccinate synthase n=32 Tax=Bacteroidales
           RepID=A6KXY6_BACV8
          Length = 402

 Score = 56.8 bits (136), Expect = 6e-07,   Method: Composition-based stats.
 Identities = 34/123 (27%), Positives = 52/123 (42%), Gaps = 9/123 (7%)

Query: 2   KRAVVVFSGGQD-STTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           K+ VV FSGG D S T +  A ++  EV+    + G     ++    E A KLGA  +  
Sbjct: 6   KKVVVAFSGGLDTSFTVMYLAKEKGYEVYAACANTGGFSEEQLKQNEENAYKLGAVKY-- 63

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI-YAYQVKAEAV 119
           + + +  E    SL      V          P +    R  +F  LA   YA ++ A+A+
Sbjct: 64  VTLDVTREYYEKSL---KYMVFGNVLRNGTYPISVSSER--IFQGLAIARYANEIGADAI 118

Query: 120 ITG 122
             G
Sbjct: 119 AHG 121


>UniRef50_Q0A9U9 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Alkalilimnicola ehrlichii MLHE-1 RepID=Q0A9U9_ALHEH
          Length = 422

 Score = 56.4 bits (135), Expect = 7e-07,   Method: Composition-based stats.
 Identities = 36/184 (19%), Positives = 65/184 (35%), Gaps = 32/184 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG---QRHRAE---IDVARELALKLGAR 56
           RA+ + SGG DS        ++   V  + F      QR  A+   I +AR+L+   G  
Sbjct: 205 RALTLLSGGIDSPVAAFLMAKRGCRVDFLHFTASPSQQRADADHTVIRLARQLSRYTGHS 264

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
              ++     +                            V  R  +  T     A +++A
Sbjct: 265 RLYMVPYDHFDMALHGDQRGHE----------------VVLFRRFVART-GEALAKRLRA 307

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A+I+G      +          + + + VS  +A  +    PL+ +DKA+  A+    G
Sbjct: 308 LALISGDSLGQVAS---------QTMENMVSTSLAVRMPIMRPLVGLDKADIVAIGRRIG 358

Query: 177 KLDL 180
             D+
Sbjct: 359 TYDI 362


>UniRef50_C5A340 P-loop ATPase, MJ1599 type n=8 Tax=Thermococcaceae
           RepID=C5A340_THEGJ
          Length = 276

 Score = 56.4 bits (135), Expect = 8e-07,   Method: Composition-based stats.
 Identities = 26/85 (30%), Positives = 42/85 (49%), Gaps = 7/85 (8%)

Query: 1   MK---RAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGA 55
           MK   +A+V +SGG+DS   L  A + Y   E+  V  D+G      I+ AR +A  LG 
Sbjct: 49  MKNVDKAIVAYSGGKDSVVALYLAKEVYKVPELEAVMIDHGLMAEEAIENARRIAEHLGV 108

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIP 80
              +VL     +++  ++L +   P
Sbjct: 109 -PFRVLRYDY-SDIFRNALLKGQSP 131


>UniRef50_A0B2R8 Putative uncharacterized protein n=4 Tax=Burkholderia cenocepacia
           RepID=A0B2R8_BURCH
          Length = 428

 Score = 56.0 bits (134), Expect = 1e-06,   Method: Composition-based stats.
 Identities = 42/236 (17%), Positives = 70/236 (29%), Gaps = 36/236 (15%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL--- 61
           V + SGG DS    +   ++      V+    Q  + + +     A+ LG     +    
Sbjct: 142 VCLLSGGLDSLVGALDLTEEGRHPLLVS----QTAKGDKETQSRFAIGLGGNDRHLQWNQ 197

Query: 62  -DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT----------FVPGRNILFLTLAAIY 110
                + ++  S+  R           AD +  T          FVP   ++ L +    
Sbjct: 198 NIRPKVEDIEGSTRGRSIGFFAFAAVAADHLATTITALPRPVEVFVPENGLISLNI---- 253

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAV-SLGMAKDIRFETPLMWIDKAETW 169
              +    V  G   T  +        F+  L      LG+   +R   P   + K E  
Sbjct: 254 --PLNPGRV--GSLSTKTTH-----PVFMARLQALWDELGIRAVLRL--PYAAMTKGEMM 302

Query: 170 ALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           A       L  V +E  +C  +       CG C  C +R            T    
Sbjct: 303 AECRASTVLADVASEATSCGRFVRNGYKHCGRCVPCLVRRASFIRANLRDDTTYVY 358


>UniRef50_UPI0001BC5A6B tRNA (5-methylaminomethyl-2-thiouridylate) -methyltransferase n=2
           Tax=Fusobacterium RepID=UPI0001BC5A6B
          Length = 345

 Score = 55.6 bits (133), Expect = 1e-06,   Method: Composition-based stats.
 Identities = 39/196 (19%), Positives = 60/196 (30%), Gaps = 38/196 (19%)

Query: 1   MKRAVVV-FSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR--AEIDVARELALKL---- 53
           MK  V++  SGG DS        ++  EV  V F          E+   +++A K     
Sbjct: 1   MKEKVILGMSGGVDSAVAAYLLQKEGYEVIAVHFSVHNTEATQKELQDCQKIADKFSLTL 60

Query: 54  -----------GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL 102
                         ++ + +       +   L  DSI       EA+     +V      
Sbjct: 61  YSYSLEKEFREKIISYYLSETGKGKTPSPCPLCDDSIKFHLLFQEAEKRKANYV------ 114

Query: 103 FLTLAAI-YAYQVKAEAVITGVCETDFSGYPD-CRDEFVKALNHAVSLGMAKDIRFETPL 160
               A   YA   +     T + E     + D C             L   K  +   PL
Sbjct: 115 ----ATGHYASISRNNICNTTLLEVKHHIHKDQC--------YMLYRLSPKKLNKILFPL 162

Query: 161 MWIDKAETWALADYYG 176
             ++KAE   LA   G
Sbjct: 163 ASLEKAEVRELARDIG 178


>UniRef50_A6Q239 tRNA-specific 2-thiouridylase mnmA n=2 Tax=Epsilonproteobacteria
           RepID=MNMA_NITSB
          Length = 339

 Score = 55.6 bits (133), Expect = 1e-06,   Method: Composition-based stats.
 Identities = 38/193 (19%), Positives = 58/193 (30%), Gaps = 24/193 (12%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARAH 58
           K+ VV  SGG DS+        +  EV  V   +  R   H+  I   +++A  LG   H
Sbjct: 3   KKIVVAMSGGVDSSYTANLLQSRGYEVIGVYMKFHPREEYHQKNIANIQKVAKHLGIEYH 62

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            +       E          +      P A          R + F  L   +A ++  + 
Sbjct: 63  VLDRTKEFQERVYQPFVDSYVAGLTPNPCAMC-------NRVMKFTEL-IEFADELGVDN 114

Query: 119 VITG--VCETDFSGYP--DCRDE---FVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
           V TG          Y   D   +   F+       +L      R   PL    K      
Sbjct: 115 VATGHYAKTDGQFIYEATDKSKDQSYFL------FNLKKEFLPRIIFPLGDWYKENVKKE 168

Query: 172 ADYYGKLDLVRNE 184
           A     L  +  +
Sbjct: 169 AMKILLLKSIAEQ 181


>UniRef50_B2A8M8 Probable tRNA sulfurtransferase n=2 Tax=Clostridia RepID=THII_NATTJ
          Length = 413

 Score = 55.2 bits (132), Expect = 2e-06,   Method: Composition-based stats.
 Identities = 35/189 (18%), Positives = 73/189 (38%), Gaps = 26/189 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYG---QRHRAEIDVARELALKLGARAH 58
           + V++ SGG DS      A+++  +V  + F  Y    QR   +++   ++  + G    
Sbjct: 185 KGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYGT--- 241

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
                     +     T     + +    ++ +  T    R  +F  +A   A + +A  
Sbjct: 242 --GPTGGFKLITNH-FTDIQKAIQN--YCSESMWVTV--MRRFMFY-IANRMAQKEQAMT 293

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           V+TG      +       + ++++ HAVS     ++    PL  +DK E  + A+  G  
Sbjct: 294 VVTGENVGQVAS------QTLESM-HAVSQ-DVVNLPILRPLAGLDKKEIMSKAETIGTY 345

Query: 179 DL---VRNE 184
           D+      +
Sbjct: 346 DISIRPYED 354


>UniRef50_B9JNY0 Putative uncharacterized protein n=1 Tax=Agrobacterium radiobacter
           K84 RepID=B9JNY0_AGRRK
          Length = 457

 Score = 54.8 bits (131), Expect = 2e-06,   Method: Composition-based stats.
 Identities = 44/235 (18%), Positives = 70/235 (29%), Gaps = 45/235 (19%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V  FS G DS                                R + +++GA+     D
Sbjct: 142 KVVTPFSDGLDSRAVTAMMTAGG------------------RSDRLIRVRVGAKT----D 179

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT- 121
                E   +      IP      +     +T V  R   F  L+ I AY +    ++  
Sbjct: 180 DRPRTEHRKAPFLT--IPFKVKSGDRPFAEST-VRSRGFKFTLLSGIAAYLLGTHDIVMP 236

Query: 122 ----GV-----CETDFSGYPD--CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
               G       +T   GY D      F   +   V+      + +  P +W  K +T  
Sbjct: 237 ESGQGALGPSLVQTSH-GYEDYRNHPLFFGRMERLVAAIFNYQVSYRLPRLWKTKGQTLG 295

Query: 171 LADYYGKLDLVRNETLTCYN-------GFKGDGCGHCAACNLRANGLNHYLADKP 218
                        +T +C+          K   CG CAAC LR    +    ++P
Sbjct: 296 EFAALPVNPNEWVKTRSCWQQNRNSSVDGKRRQCGICAACMLRRLSFHAAGLEEP 350


>UniRef50_Q8DNP8 NADPH-dependent 7-cyano-7-deazaguanine reductase n=26
          Tax=Streptococcus pneumoniae RepID=QUEF_STRR6
          Length = 154

 Score = 54.8 bits (131), Expect = 2e-06,   Method: Composition-based stats.
 Identities = 22/50 (44%), Positives = 32/50 (64%), Gaps = 1/50 (2%)

Query: 22 LQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTLLNELAV 71
          +Q Y+ V  VTF YGQ H  EI + RE+A + G R H +LD++LL ++  
Sbjct: 1  MQHYETVEAVTFAYGQHHHLEIQITREIAKEQGIR-HHILDMSLLGQITA 49


>UniRef50_Q1LLD1 Putative uncharacterized protein n=1 Tax=Cupriavidus metallidurans
           CH34 RepID=Q1LLD1_RALME
          Length = 419

 Score = 54.8 bits (131), Expect = 2e-06,   Method: Composition-based stats.
 Identities = 39/235 (16%), Positives = 65/235 (27%), Gaps = 42/235 (17%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V + SGG DS    + A+ +      +     Q    + +  R+ A  LG  A       
Sbjct: 140 VALLSGGLDSLIGGIDAVAKMRR--PIFVS--QLAYEDSERQRQYAAALGGAAWH----- 190

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLA---AIYAYQVKAEAVIT 121
                               +P A   P+T    R++ F  LA              ++ 
Sbjct: 191 -------------QQWSHRIDPAAPREPST--RARSMGFYALAVLATSLLTTTGPAEILV 235

Query: 122 ---GVCETDFSGYP------DCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
              G    +    P        R     F+K L   +   +        P  +  K E  
Sbjct: 236 PENGFISVNPPLLPGRMASLSTRTTHPLFMKQLQGLLK-AVGIPANLSMPYRFKTKGEMM 294

Query: 170 ALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                   L    +++ +C  Y  +    CG C  C +R      +     T   
Sbjct: 295 MECLDQKLLAHFASDSTSCGRYRTYNRTHCGRCVPCMVRRAAFLRWGQADDTRYK 349


>UniRef50_A3DEB6 Probable tRNA sulfurtransferase n=34 Tax=Bacteria RepID=THII_CLOTH
          Length = 392

 Score = 54.4 bits (130), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 34/190 (17%), Positives = 64/190 (33%), Gaps = 33/190 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---YGQRHRAE--IDVARELALKLGARA 57
           +AV++ SGG DS        ++  E+  V F    Y      E  I++ + LA       
Sbjct: 181 KAVLLLSGGIDSPVAGWMIAKRGVEIEAVHFYSYPYTSERAKEKVIELTKILATYCQKIN 240

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             ++             T   + + +  P  +      +  R  +   +A I A +  A 
Sbjct: 241 LHIVPF-----------TEIQLEINEKCPHEELT----IIMRRAMM-RIAEIIANKTGAL 284

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A++TG      +          + +   V       +    PL+ +DK E   +A   G 
Sbjct: 285 ALVTGESVGQVAS---------QTIQSLVVTNAVVSLPVFRPLIGMDKNEVVDIAKKIGT 335

Query: 178 LD---LVRNE 184
            +   L   +
Sbjct: 336 FETSILPYED 345


>UniRef50_B7H703 Probable tRNA sulfurtransferase n=75 Tax=Bacteria RepID=THII_BACC4
          Length = 404

 Score = 54.4 bits (130), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 33/190 (17%), Positives = 65/190 (34%), Gaps = 33/190 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF----DYGQRHR-AEIDVARELALKLGARA 57
           + +V+ SGG DS       +++   V  V F       +R +   ID+A+EL        
Sbjct: 180 KVMVLLSGGIDSPVAAYLTMKRGVSVEAVHFHSPPFTSERAKQKVIDLAQELTKYCKRVT 239

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             ++  T + +      T +      Y           +  R           A +  A 
Sbjct: 240 LHLVPFTEVQK------TINKEIPSSYSMTVMRRMMMRITER----------IAEERNAL 283

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A+ TG      +       + + ++ H ++      +    PL+ +DK E   +A+  G 
Sbjct: 284 AITTGESLGQVAS------QTLDSM-HTINEVTNYPV--IRPLITMDKLEIIKIAEEIGT 334

Query: 178 LDL---VRNE 184
            D+      +
Sbjct: 335 YDISIRPYED 344


>UniRef50_D1UGP9 Putative uncharacterized protein n=1 Tax=Burkholderia sp. CCGE1001
           RepID=D1UGP9_9BURK
          Length = 460

 Score = 54.1 bits (129), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 22/96 (22%), Positives = 33/96 (34%), Gaps = 8/96 (8%)

Query: 134 CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW-ALADYYGKLDLVRNETLTC---- 188
           C   F K L+  +     K I F  P ++  KA+    L  +   L  V  +  +C    
Sbjct: 259 CYPGFTKQLSRFLEKLTRKSIDFYHPALFQTKAQVLMQLEGHGVGLGQVLAQHWSCSYDS 318

Query: 189 ---YNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              +   +   CG C  C LR     +  A   T  
Sbjct: 319 RWAHAEHRKVHCGICGNCLLRRVAETNAQATGATAY 354


>UniRef50_Q12W34 ATPase of the PP-loop superfamily n=1 Tax=Methanococcoides burtonii
           DSM 6242 RepID=Q12W34_METBU
          Length = 267

 Score = 54.1 bits (129), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 42/194 (21%), Positives = 72/194 (37%), Gaps = 41/194 (21%)

Query: 2   KRAVVVFSGGQDSTT-CLVQALQQYDEVHCVTFDY----GQRHRAEIDVARELALKLGAR 56
           ++ +V FSGG DS+T   +      D    VT  Y     Q   +E++ A+  A  +G +
Sbjct: 18  EKVLVAFSGGVDSSTLAALAFAALGDNALAVTL-YSPMIPQ---SELEYAKRTAEVIGIK 73

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H VLD  +        L+ + I + D +                 F+      A +   
Sbjct: 74  -HIVLDHDI--------LSDERIRINDPQRCYYCKKQ---------FVKKLKGVASEEGL 115

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI--DKAETWALADY 174
             ++ G   ++ +G    R   ++AL  A            TP +     K E   +A Y
Sbjct: 116 NVIMEGTNASEITG---RRPG-LEALREA-------GSSIFTPYVDFDVSKREVRQMASY 164

Query: 175 YGKLDLVRNETLTC 188
            G LD+    + +C
Sbjct: 165 LG-LDVANRPSNSC 177


>UniRef50_C7X359 Putative uncharacterized protein n=1 Tax=Enterococcus faecalis
           Merz96 RepID=C7X359_ENTFA
          Length = 427

 Score = 54.1 bits (129), Expect = 3e-06,   Method: Composition-based stats.
 Identities = 39/243 (16%), Positives = 74/243 (30%), Gaps = 40/243 (16%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YG--QRHRAEIDVARELALKLGARAHKVL 61
           V +FSGG DS    +  L+     + +    YG   +               G +   V 
Sbjct: 140 VCMFSGGLDSFIGSIDILRGQTTSNTLFVSHYGGGSKGTK------------GYQDLLVK 187

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++         S            P     P T    R+  F+  A   A       +I 
Sbjct: 188 EMINHFSSLSLSNFFSFYSAAMKTPTGQDEPTT----RSRSFMFFAHAIALTPDNAKLII 243

Query: 122 ---GVCETDFSGY------PDCR---DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
              G    +   Y         R     ++      +++ +  ++R E P  ++ K +  
Sbjct: 244 PENGFVSLNIPLYFSRNGSSSTRTTHPFYMNLFQKILNI-LGINVRLENPYQFLTKGQMM 302

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGD--------GCGHCAACNLRANGLNHYLADKPTVM 221
                   L      T++C +  +           CG+C  C +R   +N + +  PT+ 
Sbjct: 303 NNCLDKEFLSRNIINTMSCSHPEQARWSGCSDPIHCGYCIPCTIRRASINEFCSPDPTLY 362

Query: 222 AAM 224
             +
Sbjct: 363 RYL 365


>UniRef50_B1L455 ExsB family protein n=1 Tax=Candidatus Korarchaeum cryptofilum OPF8
           RepID=B1L455_KORCO
          Length = 260

 Score = 54.1 bits (129), Expect = 4e-06,   Method: Composition-based stats.
 Identities = 32/176 (18%), Positives = 60/176 (34%), Gaps = 37/176 (21%)

Query: 4   AVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
            ++  SGG DS+T      +   D+   VT     +   EI+ AR +A ++G R H +++
Sbjct: 16  VIIALSGGLDSSTLAALCKKLLGDKAIAVTVASELQPEEEIEGARRVAEEIGIR-HILIE 74

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V         SL ++ +     +         F   R           A ++    +  G
Sbjct: 75  V---------SLLKEEVARNGPDRCYHCKKAMFRRIR---------ELAEELGVREIFDG 116

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWID--KAETWALADYYG 176
              +D       R            +G  +++   +P   +   K E   +A   G
Sbjct: 117 TNASDSW-----RPG----------IGALRELGIRSPWAELGFRKEEIREIARNLG 157


>UniRef50_D1B2I0 Queuosine synthesis-like protein n=2 Tax=Campylobacterales
           RepID=D1B2I0_SULD5
          Length = 261

 Score = 53.7 bits (128), Expect = 5e-06,   Method: Composition-based stats.
 Identities = 39/178 (21%), Positives = 65/178 (36%), Gaps = 36/178 (20%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQ-QYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +R +V FSGG DS+  L  A     + V  ++        +EI+ AR +A ++GAR H V
Sbjct: 16  ERLIVAFSGGVDSSFLLKTAYDVLGENVLAISLQTPYIAESEIEEARRIADEIGAR-HLV 74

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           L    + EL  +   ++   +  +                 LF +L   +A Q     + 
Sbjct: 75  LVKPWMEELRTNP--KERCYLCKH----------------ALFSSL-TTFAQQRDFRVIA 115

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMW--IDKAETWALADYYG 176
            G    D              +          ++   TPL+   + KAE   L+   G
Sbjct: 116 EGSNVDDT-------------MEKRPGRVALLELSILTPLLDVGLSKAEIRLLSKAVG 160


>UniRef50_C9RQ01 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Fibrobacter succinogenes subsp. succinogenes S85
           RepID=C9RQ01_FIBSS
          Length = 366

 Score = 53.7 bits (128), Expect = 5e-06,   Method: Composition-based stats.
 Identities = 47/203 (23%), Positives = 66/203 (32%), Gaps = 39/203 (19%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-----------------DYGQRHRAEID 44
           KR  V  SGG DS        +Q  EV  +T                   YG      I+
Sbjct: 5   KRVAVGLSGGVDSALSAYLLKKQGYEVVGMTMATWDDSVKMPAVEGREGCYGPSEDKNIE 64

Query: 45  VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP-GRNILF 103
            A+ +A +LG   H V+ V            R+ +     E  A   PN  V   ++I F
Sbjct: 65  EAKLVAERLGI-PHYVVPV-------AEDYKREVLDYFRAEYRAGRTPNPCVRCNQSIKF 116

Query: 104 LTLAAIYAYQVKAEAVITGV---CETDFSGYPDCRDEFVKALNH-------AVSLGMAKD 153
             L    A ++  +            DF   PD      +AL+           L   + 
Sbjct: 117 GALQHA-ARKLGIDFDYFATGHYARLDFKN-PD-VPFLYEALDEHKDQTYFLSRLSAEQL 173

Query: 154 IRFETPLMWIDKAETWALADYYG 176
                PL  + KA+  ALA   G
Sbjct: 174 STVIFPLGGMQKADVKALAKEIG 196


>UniRef50_B3QQJ9 Putative uncharacterized protein n=1 Tax=Chlorobaculum parvum NCIB
           8327 RepID=B3QQJ9_CHLP8
          Length = 190

 Score = 53.7 bits (128), Expect = 5e-06,   Method: Composition-based stats.
 Identities = 30/110 (27%), Positives = 38/110 (34%), Gaps = 17/110 (15%)

Query: 108 AIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI------------- 154
           A YA       +  GV     S YP  R  + +     VS G +  I             
Sbjct: 58  ARYASWKDLCEIEIGV--EGASSYP-RRPVYAQLEKELVSQGDSFIIGDTLDDDLFAFFR 114

Query: 155 RFETPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACN 204
            F  P++   K     LA   G  DL+ N T  C+N   G  CG C  C 
Sbjct: 115 NFSFPIINKPKFRLLQLAKDQGFYDLMLN-TWFCHNPRNGKPCGKCLPCR 163


>UniRef50_A9A452 ExsB family protein n=2 Tax=Thaumarchaeota RepID=A9A452_NITMS
          Length = 265

 Score = 53.3 bits (127), Expect = 6e-06,   Method: Composition-based stats.
 Identities = 36/189 (19%), Positives = 66/189 (34%), Gaps = 35/189 (18%)

Query: 3   RAVVVFSGGQDSTTCLVQA-LQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + ++  SGG DS      A  +  D    VT DY      E+  A+++  ++G     + 
Sbjct: 16  KVMIALSGGVDSALVAYAAYQKLGDFAIAVTADYKTLSEEELQTAKQICSEIGITQFFL- 74

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                +EL     T++      +     G+            L L    A +   + ++ 
Sbjct: 75  ---DYDELENEEFTKNDSTRCFHCRLELGV----------HLLKL----AKEHHVDVIVD 117

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLM--WIDKAETWALADYYGKLD 179
           G    D   Y   R   + AL           IR  +PL+   + K++   +A   G L 
Sbjct: 118 GTNLDDLGEY---RPG-IDALRQ-------NGIR--SPLVETNLSKSQIRHIAKSVG-LS 163

Query: 180 LVRNETLTC 188
           +    + +C
Sbjct: 164 VHDKPSNSC 172


>UniRef50_A8AA65 Argininosuccinate synthase n=5 Tax=Archaea RepID=ASSY_IGNH4
          Length = 396

 Score = 53.3 bits (127), Expect = 7e-06,   Method: Composition-based stats.
 Identities = 22/64 (34%), Positives = 34/64 (53%), Gaps = 5/64 (7%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHR-AEIDVARELALKLGARAH 58
          M + V+ +SGG D++  +    ++   EV  VT D GQ+    EI+   E A K GA  H
Sbjct: 1  MSKIVLAYSGGLDTSVAVAWLKEKFGAEVITVTVDVGQKEDFKEIE---ERAYKAGASKH 57

Query: 59 KVLD 62
           ++D
Sbjct: 58 YLID 61


>UniRef50_Q0AM89 Putative uncharacterized protein n=1 Tax=Maricaulis maris MCS10
           RepID=Q0AM89_MARMM
          Length = 439

 Score = 53.3 bits (127), Expect = 7e-06,   Method: Composition-based stats.
 Identities = 39/234 (16%), Positives = 64/234 (27%), Gaps = 37/234 (15%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTF----DYGQRHRAEIDVARELALKLGARAHKV 60
           V +FSGG DS    +  +        V+     D G            L   L       
Sbjct: 149 VCLFSGGMDSLIGAIDRIADGHRPLLVSRSTRGDGG--------YQTYLHGMLPEVPSLR 200

Query: 61  LDVTLLNELAVSSLTRDSIPVPDY----------EPEADGIPNTFVPGRNILFLTLAAIY 110
           ++  +  EL     TR    V                 D I    +P  N  F++L    
Sbjct: 201 INDDIRGELQGEPSTRTRSIVFLALAACAASAISRLHNDSIVPLIIPE-NG-FISLNPPL 258

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
             +        G   T           F+ +L       +      E P  +  K E   
Sbjct: 259 TVRR------IGALSTRTVH-----PHFISSLQAIFDE-VGIPAAIENPYFFKTKGEALR 306

Query: 171 LADYYGKLDLVRNETLTCYNGFKG-DGCGHCAACNLRANGLNHYLADKPTVMAA 223
                  +  + ++T +C    +    CG C  C +R +  +       T  + 
Sbjct: 307 ECRDQPLVTKLISKTTSCGKWKRYWQQCGRCVPCLIRRSAFHAAGIADETATSK 360


>UniRef50_Q7URE0 Putative uncharacterized protein n=1 Tax=Rhodopirellula baltica
           RepID=Q7URE0_RHOBA
          Length = 316

 Score = 52.9 bits (126), Expect = 8e-06,   Method: Composition-based stats.
 Identities = 37/177 (20%), Positives = 56/177 (31%), Gaps = 40/177 (22%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARA 57
           M   VV FSGG DS+     A +   +V  VT    Q     R ++D AR +A ++G   
Sbjct: 28  MGGLVVAFSGGVDSSVVAAAAHRTGRDVVAVT---AQSPAVARWQLDWARRVAEQVGI-T 83

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           H+ +             +  S    ++         T                A Q  A 
Sbjct: 84  HQFVSTDE---------SDRSSYRRNHSDRCFYCKQT--------LYEFLTPIAEQRGAT 126

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI--DKAETWALA 172
            +++G    D     D R                   R +TPL  +   K+    LA
Sbjct: 127 -IVSGTNADDLG---DHRPGITA----------GTQARVQTPLADLGIGKSAVRQLA 169


>UniRef50_D0MEN6 Asparagine synthase (Glutamine-hydrolyzing) n=1 Tax=Rhodothermus
           marinus DSM 4252 RepID=D0MEN6_RHOM4
          Length = 554

 Score = 52.9 bits (126), Expect = 8e-06,   Method: Composition-based stats.
 Identities = 39/202 (19%), Positives = 64/202 (31%), Gaps = 39/202 (19%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +R  V+ SGG DST      + +  EV       GQ   +++ + + LA  LG   H V 
Sbjct: 249 RRVAVLLSGGVDSTLLAKALVDRGLEVTGYCV--GQPEASDVQLVKRLARTLGLSVH-VT 305

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++T     A       +I +         IP           + LA   A +     + +
Sbjct: 306 ELTPELVAAELPEIIRAIEMNGPVQVGAAIP-----------MYLATKTAARDGHRVLYS 354

Query: 122 GVCETD-FSGY--------------------PDCRDEFVKALNHAVSLGMAKDIRFETPL 160
           G    + F+GY                     D    +V  L       MA  +    P 
Sbjct: 355 GQAADELFAGYDWYREVLQREGPLALHARLWEDIDALYVDTLEREDRTSMAHSVELRAPF 414

Query: 161 MWIDKAETWALADYYG-KLDLV 181
           +     +   +A     +L L 
Sbjct: 415 LD---RDLLRVAMRMDPRLKLP 433


>UniRef50_B8D4I6 tRNA sulfurtransferase n=1 Tax=Desulfurococcus kamchatkensis 1221n
           RepID=THII_DESK1
          Length = 482

 Score = 52.9 bits (126), Expect = 9e-06,   Method: Composition-based stats.
 Identities = 43/201 (21%), Positives = 70/201 (34%), Gaps = 48/201 (23%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH--RAEIDVARELALK-LGARAHK 59
           RA+V+FSGG DS        ++  EV  + +  G     R    VAR+L+ + L +   +
Sbjct: 174 RALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGSSEVSRQAFSVARKLSEEWLSSYNPR 233

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTF--VPGRNILFLTLAAIYAYQVKAE 117
            + V                  P      + I  ++  V  R  L   +A   A ++   
Sbjct: 234 FITVDF---------------TPLIAEIEERIEWSYRQVVLR-ALMYMVADKIATELGYN 277

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFET----------PLMWIDKAE 167
            ++TG                 +AL+ A S  +A  +  E+          PL+  DK E
Sbjct: 278 TIVTG-----------------EALSQASSQTLANLVAVESAVSPRSIILRPLIGFDKEE 320

Query: 168 TWALADYYGKLDLVRNETLTC 188
               +   G  D       TC
Sbjct: 321 IIEYSRRIGLYDYSSRVAETC 341


>UniRef50_C8WW19 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Alicyclobacillus acidocaldarius RepID=C8WW19_ALIAD
          Length = 400

 Score = 52.5 bits (125), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 40/184 (21%), Positives = 72/184 (39%), Gaps = 32/184 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF----DYGQRHRAEIDVARELALKLGA--R 56
           + +V+ SGG DS     +A+++   +  V F       +R + +++    +  K G   +
Sbjct: 179 KGLVLLSGGIDSPVAAWKAMKRGLALELVHFHSFPFTSERAQQKVEDLAAILTKWGGPMK 238

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H V    +  E+  S+               +G+  T +  R  + +  A+  A +V A
Sbjct: 239 LHLVSVTAIQAEIQRSA--------------PEGLR-TILLRR--MMVRAASRIADEVGA 281

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A++TG      +       + + ALN       A D     PL+  DK E   LA   G
Sbjct: 282 GALVTGDSLGQVAS------QTLSALNAVDR---ATDHTILRPLIAEDKLEIIELAKRMG 332

Query: 177 KLDL 180
             D+
Sbjct: 333 TYDI 336


>UniRef50_A8EUQ0 Thiamine biosynthesis protein ThiI n=4 Tax=Proteobacteria
           RepID=A8EUQ0_ARCB4
          Length = 488

 Score = 52.5 bits (125), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 33/182 (18%), Positives = 57/182 (31%), Gaps = 38/182 (20%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
            + + SGG DST      +++  + H + F+ G            LA ++G +       
Sbjct: 190 VISLMSGGFDSTVASYLTMKRGIKTHFIFFNLG-----------GLAHEIGVKQ----VA 234

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPG------RNILFLTLAAIYAYQVKAE 117
             L     SS     I VP  +  +    +   P       R  L L  +   A  +  +
Sbjct: 235 YYLWNKFGSSHRVSFISVPFEDVVSQIFKDVSQPYMGVMLKR--LMLLASEKIADSLGID 292

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR---FETPLMWIDKAETWALADY 174
           A++TG      S                 +L +           PL  + K +   +A  
Sbjct: 293 ALVTGESVAQVSS------------QTLRNLALIDQCTNKLVLRPLSMMSKPDIIEIAQK 340

Query: 175 YG 176
            G
Sbjct: 341 IG 342


>UniRef50_A1U6U9 tRNA sulfurtransferase n=11 Tax=Gammaproteobacteria
           RepID=THII_MARAV
          Length = 484

 Score = 52.5 bits (125), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 38/185 (20%), Positives = 61/185 (32%), Gaps = 38/185 (20%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           ++  + + SGG DS+      +++    H + F+                  LG  AH+V
Sbjct: 178 VETVMTLISGGYDSSVAAYLMMRRGIRSHYLFFN------------------LGGAAHEV 219

Query: 61  L---DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNI------LFLTLAAIYA 111
                   L E   SS     I VP     A+ + +  V  R+       + L  AA  A
Sbjct: 220 GVRQVAHYLWERYGSSHNVKFISVPFEGVVAEIMRS--VNHRHWGVVLKRMMLKAAAEIA 277

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
               A  ++ G      S       + +  LN               PL+ +DK E   +
Sbjct: 278 RDYNASGLVMGDAVAQVSS------QTLTNLNVVDRASDE---VVLRPLIAMDKQEIIRI 328

Query: 172 ADYYG 176
           A   G
Sbjct: 329 AKDIG 333


>UniRef50_Q5SH30 NH(3)-dependent NAD(+) synthetase n=3 Tax=Thermus
           RepID=Q5SH30_THET8
          Length = 281

 Score = 52.5 bits (125), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 38/178 (21%), Positives = 61/178 (34%), Gaps = 33/178 (18%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++A+V  SGG DS T L  A++    + VH +   + +      + A  +A   G    +
Sbjct: 38  EKAIVAVSGGVDSATTLALAVRALGAERVHALFLPHRESSPTSREHAYLVAETFGVALEE 97

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIY-AYQVKAEA 118
           V    ++     ++LT D  P       A          R I+    +  Y A  +    
Sbjct: 98  VDITPMVE--GYAALTPDLTPHRKGNVMAR--------ARMIVLFDKSEAYRALPLG--- 144

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
             TG       GY      F          G         PL  + K + W LA++ G
Sbjct: 145 --TGNKTERLFGY------F-------TWHGDDTPP--VNPLGDLYKTQVWRLAEHLG 185


>UniRef50_B5XVJ1 Putative uncharacterized protein n=1 Tax=Klebsiella pneumoniae 342
           RepID=B5XVJ1_KLEP3
          Length = 421

 Score = 52.5 bits (125), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 38/234 (16%), Positives = 74/234 (31%), Gaps = 46/234 (19%)

Query: 7   VFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKL-GARAHKVLDVTL 65
           + SGG DS    +  + +  +   +     Q    + +  R  A  L G  +H       
Sbjct: 143 LLSGGLDSLIGGIDLVAKGRK--PLFVS--QLAHKDSERQRAYASMLMGNGSHY------ 192

Query: 66  LNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA--VIT-- 121
             + +     + S             P+T   GR++ F   A + + ++      +    
Sbjct: 193 --QWSHGISFKGSRE-----------PST--RGRSLAFYAFALLASTKINLPKPTIFIPE 237

Query: 122 -GVCETDFSGYP------DCR---DEFVKALNHAV-SLGMAKDIRFETPLMWIDKAETWA 170
            GV   +    P        R     F+  L   + +LG+   ++ E P  +  K E   
Sbjct: 238 NGVISINPPLVPGRVSSLSTRTTHPMFIDMLQRVLDNLGI--HVQLELPYRFKTKGEMMR 295

Query: 171 LADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLAD-KPTVM 221
                  L     ++ +C  +  +    CG C  C +R      +  +   T  
Sbjct: 296 ECLDQDLLQKWAADSTSCGRFRTYNRQHCGRCVPCLIRRAAFIAWKPEGDTTSY 349


>UniRef50_A1ZJJ3 Asparagine synthase family n=1 Tax=Microscilla marina ATCC 23134
           RepID=A1ZJJ3_9SPHI
          Length = 563

 Score = 52.5 bits (125), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 16/60 (26%), Positives = 31/60 (51%), Gaps = 1/60 (1%)

Query: 2   KRAVVVFSGGQDSTTCLV-QALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           K   V  SGG DS+      A     ++H  +  +G+++R E+  A+++A +   + H+V
Sbjct: 221 KEIGVFLSGGLDSSILAARVAQLSSKKIHTYSIHFGKKYRNELHYAQKVAQRYQTQHHEV 280


>UniRef50_C8WPE1 ExsB family protein n=1 Tax=Eggerthella lenta DSM 2243
           RepID=C8WPE1_EGGLE
          Length = 210

 Score = 52.1 bits (124), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 48/197 (24%), Positives = 72/197 (36%), Gaps = 42/197 (21%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH----RAEIDVARELALKLGARAHKVL 61
           V FSGG DS+  L  AL+   +V      YG R       EI+ A+ LA +LGA   +++
Sbjct: 27  VAFSGGCDSSFLLAAALRAGCDVRA----YGVRTAFQPAFEIEDAQRLAAELGA-DFELV 81

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D  +L +  + +            P+      TF      +F T+ A  A     E +  
Sbjct: 82  DADVLAQGEICA----------NGPDRCYRCKTF------IFSTILARMAED-GFEVLAD 124

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL--MWIDKAETWALADYYGKLD 179
           G   TD    P  R  F         LG        +PL    + K +  A +   G   
Sbjct: 125 GTNVTDD---PANRPGF----QALAELG------VVSPLRRAGMTKDDVRAASRELGLFT 171

Query: 180 LVRNETLTCYNGFKGDG 196
                + +C     G+G
Sbjct: 172 AD-KPSFSCIAVHVGEG 187


>UniRef50_C9YDI6 Putative uncharacterized protein n=1 Tax=Curvibacter putative
           symbiont of Hydra magnipapillata RepID=C9YDI6_9BURK
          Length = 647

 Score = 52.1 bits (124), Expect = 1e-05,   Method: Composition-based stats.
 Identities = 29/130 (22%), Positives = 51/130 (39%), Gaps = 21/130 (16%)

Query: 8   FSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-----IDVARELALKLGARAHKVLD 62
            SGG DS+  +  A +Q  ++ C + D  Q  + E     +  AR +A  LG   H V+ 
Sbjct: 264 LSGGLDSSAVVAFAREQNPDIQCYSIDV-QGAQDEGFADDLPYARRVAQHLGVPLH-VVQ 321

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V   +           +  P  +P A  +   ++           +  A Q   + +++G
Sbjct: 322 VDPASMAQDLQAMVIQLDEPLADPAALNVR--YI-----------SALARQQGIKVLLSG 368

Query: 123 VCETD-FSGY 131
               D F+GY
Sbjct: 369 TAGDDLFTGY 378


>UniRef50_D1CE79 Argininosuccinate synthase n=2 Tax=Bacteria RepID=D1CE79_THET1
          Length = 417

 Score = 52.1 bits (124), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 29/123 (23%), Positives = 50/123 (40%), Gaps = 11/123 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++  + +SGG DS  CLV   ++Y  +E+  +T D GQ  + E++ + + A  LG     
Sbjct: 18  RKVALAYSGGLDSALCLVLLREKYGAEEIIPITVDVGQ-GQDEVEQSFKKAEVLGVEPLL 76

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
              +   +E     LT+       Y         T    R  L     A  A ++  +A+
Sbjct: 77  ---IDAKSEFTNEWLTKAIRANSSYNGYPVSTSMT----R-QLIAAKVAQKAVELGCDAL 128

Query: 120 ITG 122
             G
Sbjct: 129 AEG 131


>UniRef50_UPI0001C31893 argininosuccinate synthase n=1 Tax=Conexibacter woesei DSM 14684
           RepID=UPI0001C31893
          Length = 431

 Score = 51.7 bits (123), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 32/132 (24%), Positives = 56/132 (42%), Gaps = 24/132 (18%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYD-EVHCVTFDYGQRHRAE-IDVARELALKLGARAH 58
           ++R ++++SGG D++  L     +Y  EV C+T + GQ    E  DV ++ A ++GA   
Sbjct: 29  VRRVLLLYSGGLDTSVMLKWIQDEYRAEVVCLTVNLGQ--PGEDYDVIKDKARRIGAIDT 86

Query: 59  KVLDVT-------LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
            V+D         +   +  ++L     P+      A G P         L   LA  YA
Sbjct: 87  HVVDAREVFAHEFIKPAIKANALYGGGYPL----FTALGRP---------LIAKLAVEYA 133

Query: 112 YQVKAEAVITGV 123
            +   + +  G 
Sbjct: 134 RRTGCDTIAHGC 145


>UniRef50_A5CYW2 ATP-utilizing enzymes of the PP-loop superfamily n=25 Tax=cellular
           organisms RepID=A5CYW2_PELTS
          Length = 359

 Score = 51.7 bits (123), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 44/182 (24%), Positives = 63/182 (34%), Gaps = 46/182 (25%)

Query: 4   AVVVFSGGQDSTTCLVQALQ-QYDEVHCVTFDYGQRHRAEI----DVAR--ELALKLGAR 56
            +V FSGG DST  L  A     D+V  VT        +EI    + A   ELA  +GA 
Sbjct: 108 VLVAFSGGADSTLLLKVARDVLGDKVLAVT------AASEIYPPGEAAEAGELARAIGAE 161

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H V     L     S+  R++ P   Y  + +                     A +   
Sbjct: 162 -HIVFTAKGLK----SAAFRNNPPDRCYHCKKEIYTEL-------------LKLARERGI 203

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL--MWIDKAETWALADY 174
           + V  GV   D + Y   R          +  G+   ++   PL    + K E  AL+  
Sbjct: 204 KCVADGVNADDAADY---RPG--------IRAGIELGVK--APLKEAGLTKQEIHALSRQ 250

Query: 175 YG 176
            G
Sbjct: 251 LG 252


>UniRef50_C7M0G9 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase n=1
           Tax=Acidimicrobium ferrooxidans DSM 10331
           RepID=C7M0G9_ACIFD
          Length = 341

 Score = 51.7 bits (123), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 48/198 (24%), Positives = 69/198 (34%), Gaps = 41/198 (20%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-------DYGQRHRAEIDVARELALKL 53
           M R VV  SGG DS+   + A +   +V  VT        D G     +++ AR +A  L
Sbjct: 1   MARVVVAMSGGVDSSVAALMAREAGHDVIGVTLRLTEAAGDRGCCSVGDVEDARTVAALL 60

Query: 54  GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP------GRNILFLTLA 107
           G   H V D+            R        EP  D     + P       R  + +T  
Sbjct: 61  GI-NHIVADM------------RAEFHRAVVEPYLDAHRRGWTPNPCMSCNR-YVKVTAL 106

Query: 108 AIYAYQVKAEAVITGVCET--DFSGYPDCRDEFV----KALNHAVSLGMAKD---IRFET 158
           A +A ++ A  ++TG   T  D S     R   V    +  + +  L         R   
Sbjct: 107 AAWAQRLGASRLVTGHYATVLDGS-----RPMLVRGRDRRKDQSYVLADVPPEVLARLWL 161

Query: 159 PLMWIDKAETWALADYYG 176
           PL  + K E    A   G
Sbjct: 162 PLGELSKDEVRTRAHAAG 179


>UniRef50_A7HL33 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Thermotogaceae RepID=A7HL33_FERNB
          Length = 389

 Score = 51.7 bits (123), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 38/178 (21%), Positives = 72/178 (40%), Gaps = 19/178 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RA+++ SGG DS      A+++  E+  +TF             +++   LG    + L 
Sbjct: 176 RALLLLSGGIDSPVAGWYAMRRGLELQTLTFL--SPPMTTEKSVQKILD-LGRVLARYLP 232

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
             L   +    LT   + + +  P+   +    +  R  +   +A + A +VKA+A+ITG
Sbjct: 233 NGLRMWIV--PLTEVQMYIKENAPDEYSL----ILQRRSMM-RIANMIARRVKAKAIITG 285

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 +          + L +  ++  A  +    PL+  DK ET   A   G  ++
Sbjct: 286 ENLGQVAS---------QTLTNMAAIEDASSLLVLRPLIGFDKIETTNKAKEIGTFEI 334


>UniRef50_D2RFP8 ExsB family protein n=1 Tax=Archaeoglobus profundus DSM 5631
           RepID=D2RFP8_ARCPR
          Length = 259

 Score = 51.7 bits (123), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 37/180 (20%), Positives = 62/180 (34%), Gaps = 43/180 (23%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTF------DYGQRHRAEIDVARELALKLGARA 57
            VV+FSGG DS T           V  VT        Y       ++ A+ +A ++G   
Sbjct: 19  VVVMFSGGVDSVTLTKLCKDVIKRVFAVTVVSEVLSKYD------VEYAKSVAKEIGV-D 71

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           H +L++ LLN+         +     Y  +                + +   +A  V  E
Sbjct: 72  HYILEIDLLNDEN----FVRNDENRCYFCKKK-------------MIRMVKEFASAVGVE 114

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD-IRFETPLMWIDKAETWALADYYG 176
            ++ G   ++ + Y   R  +       V  G+    I        IDK E  ++A   G
Sbjct: 115 CIVEGTNVSELNEY---RPGY----RAVVEEGVYSPWIEV-----GIDKEEIRSIAKKLG 162


>UniRef50_C9LN41 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
          Tax=Dialister invisus DSM 15470 RepID=C9LN41_9FIRM
          Length = 350

 Score = 51.7 bits (123), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 21/63 (33%), Positives = 32/63 (50%), Gaps = 3/63 (4%)

Query: 2  KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
          K+ VV  SGG DST       +   +V+  T +  Q    ++  A+++A  LG   HKV+
Sbjct: 5  KKVVVAMSGGVDSTLTARLLQEAGYDVYGATMN--QFDGQDMTEAQKIADFLGI-PHKVI 61

Query: 62 DVT 64
          DV 
Sbjct: 62 DVR 64


>UniRef50_Q8TUR4 Predicted ATPase of the PP-loop superfamily implicated in cell
           cycle control n=1 Tax=Methanopyrus kandleri
           RepID=Q8TUR4_METKA
          Length = 294

 Score = 51.4 bits (122), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 25/82 (30%), Positives = 36/82 (43%), Gaps = 6/82 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVAREL--ALKL-GARAH 58
           ++ +V  SGG DS   L  AL+  DEV  +T D G  H    +  R +  A ++ G    
Sbjct: 52  EKVLVAVSGGLDSLAALGLALRICDEVVALTVDTGALHP---EAWRRIILARRMSGIEWE 108

Query: 59  KVLDVTLLNELAVSSLTRDSIP 80
            + D     +L    LTR   P
Sbjct: 109 IIGDQKPFLKLFEERLTRAESP 130


>UniRef50_B7J5X6 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Acidithiobacillus
           RepID=MNMA_ACIF2
          Length = 352

 Score = 51.4 bits (122), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 46/200 (23%), Positives = 71/200 (35%), Gaps = 49/200 (24%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR----AEIDVARELALKLGARA 57
           K+A++  SGG DS    +    Q  E+  VT     R R     +I+ A E+  +LG   
Sbjct: 4   KKALIALSGGVDSAVAALLMQGQGYELTGVTMRLWPRSRCCDEKDIEDAAEICARLGI-P 62

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP-GRNILFLTLAAIYA--YQV 114
           + VLD          +  R  + V   E +A   PN   P  R   FL   A+ A   ++
Sbjct: 63  YTVLD-------YREAFRRQVVDVFVAEYQAGRTPN---PCARCNQFLKFDALLAEGEKL 112

Query: 115 KAEAVITGV------------------CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRF 156
            A  + TG                      D S +               ++G     R 
Sbjct: 113 GATMLATGHYARLAETSSGTALLRGRDHAKDQSYF-------------LFAIGAGILSRL 159

Query: 157 ETPLMWIDKAETWALADYYG 176
             P+  ++K E  A+A  +G
Sbjct: 160 RFPVGGMNKDEVRAMARKHG 179


>UniRef50_C7DBG3 tRNA(Ile)-lysidine synthase n=1 Tax=Thalassiobium sp. R2A62
           RepID=C7DBG3_9RHOB
          Length = 422

 Score = 51.4 bits (122), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 25/71 (35%), Positives = 31/71 (43%), Gaps = 7/71 (9%)

Query: 4   AVVVFSGGQDSTTCLV----QALQQYDEVHCVTFDYGQR--HRAEIDVARELALKLGARA 57
             V  SGG DST  L+     A      VH VT D+GQR   + E     EL  +LG   
Sbjct: 32  VGVAVSGGSDSTALLLACVEWAKSNGVAVHAVTVDHGQRVEAKQEAIAVSELTAELGVE- 90

Query: 58  HKVLDVTLLNE 68
           H +L +  L  
Sbjct: 91  HSILTLETLEA 101


>UniRef50_C6CRM5 tRNA(Ile)-lysidine synthetase n=1 Tax=Paenibacillus sp. JDR-2
           RepID=C6CRM5_PAESJ
          Length = 475

 Score = 51.4 bits (122), Expect = 2e-05,   Method: Composition-based stats.
 Identities = 35/195 (17%), Positives = 65/195 (33%), Gaps = 43/195 (22%)

Query: 3   RAVVVFSGGQDSTTCLVQ----ALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGA 55
           R V   SGG DS   L      A Q+   V     ++G R    R E +V R+ A KLG 
Sbjct: 27  RIVAAVSGGPDSMALLHLLWQAARQEQLTVIAAHVNHGFRKDASRQEAEVVRQYAEKLGV 86

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
              +++++ +   +  + +   S                                A +  
Sbjct: 87  -PFEMIELDMPAYIEETGINAQSAAREKRYAFLHET-------------------AVKYG 126

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMA--------KDIRFETPLMWIDKAE 167
           A A+       D +         ++ +  A   G+A        K++    PL+ ++K++
Sbjct: 127 ASAIALAHHADDQA-----ETVLMRVIRGAGLTGLAGMAPKRKEKNVELIRPLLRMNKSD 181

Query: 168 TWALADYYGKLDLVR 182
                 Y  + D+  
Sbjct: 182 IVR---YCSERDVPY 193


>UniRef50_P49915 GMP synthase [glutamine-hydrolyzing] n=87 Tax=Eumetazoa
           RepID=GUAA_HUMAN
          Length = 693

 Score = 51.0 bits (121), Expect = 3e-05,   Method: Composition-based stats.
 Identities = 22/97 (22%), Positives = 37/97 (38%), Gaps = 3/97 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQ--QYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + +V+ SGG DST C     +    ++V  V  D G   + E     E   KLG +  KV
Sbjct: 238 KVLVLLSGGVDSTVCTALLNRALNQEQVIAVHIDNGFMRKRESQSVEEALKKLGIQV-KV 296

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP 97
           ++         ++L          +  +  +  T  P
Sbjct: 297 INAAHSFYNGTTTLPISDEDRTPRKRISKTLNMTTSP 333


>UniRef50_Q2LWA6 tRNA-specific 2-thiouridylase mnmA 1 n=1 Tax=Syntrophus
          aciditrophicus SB RepID=MNMA1_SYNAS
          Length = 372

 Score = 51.0 bits (121), Expect = 3e-05,   Method: Composition-based stats.
 Identities = 23/70 (32%), Positives = 31/70 (44%), Gaps = 10/70 (14%)

Query: 2  KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR--------AE-IDVARELALK 52
          KR ++  SGG DS+   +   ++  EV  VT   G R           E I+ AR +   
Sbjct: 21 KRVLLAISGGVDSSVAALLLKEEGYEVAGVTMCLGVREEENKVRCCGREAIEDARGVCEI 80

Query: 53 LGARAHKVLD 62
          LG   H VLD
Sbjct: 81 LGI-PHYVLD 89


>UniRef50_Q1AS35 Argininosuccinate synthase n=69 Tax=cellular organisms
          RepID=ASSY_RUBXD
          Length = 409

 Score = 51.0 bits (121), Expect = 3e-05,   Method: Composition-based stats.
 Identities = 24/61 (39%), Positives = 34/61 (55%), Gaps = 2/61 (3%)

Query: 2  KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
          K+ V+ +SGG D++ CL    +Q  E + +  D GQ   AE    RE AL++GAR   V 
Sbjct: 5  KKVVLAYSGGLDTSVCLKWFEEQGAEPYALYLDLGQGEPAE--DVREKALRIGAREVFVW 62

Query: 62 D 62
          D
Sbjct: 63 D 63


>UniRef50_A7GTS8 Probable tRNA sulfurtransferase n=5 Tax=Firmicutes RepID=THII_BACCN
          Length = 403

 Score = 51.0 bits (121), Expect = 3e-05,   Method: Composition-based stats.
 Identities = 33/190 (17%), Positives = 65/190 (34%), Gaps = 33/190 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF----DYGQRHR-AEIDVARELALKLGARA 57
           + +V+ SGG DS       +++   V  V F       +R +   ID+A+EL        
Sbjct: 180 KVMVLLSGGIDSPVAAYLTMKRGVSVEAVHFHSPPFTSERAKQKVIDLAQELTKYCKRVT 239

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             ++  T + +      T +      Y           +  R           A +  A 
Sbjct: 240 LHLVPFTEVQK------TINKEIPSSYTMTVMRRMMMRITER----------IAEERNAL 283

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A+ TG      +       + + ++ H ++      +    PL+ +DK E   +A+  G 
Sbjct: 284 AITTGESLGQVAS------QTLDSM-HTINEVTNYPV--LRPLITMDKLEIIKIANEIGT 334

Query: 178 LDL---VRNE 184
            D+      +
Sbjct: 335 YDISIRPYED 344


>UniRef50_A8ES35 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Epsilonproteobacteria
           RepID=MNMA1_ARCB4
          Length = 345

 Score = 51.0 bits (121), Expect = 4e-05,   Method: Composition-based stats.
 Identities = 37/199 (18%), Positives = 65/199 (32%), Gaps = 38/199 (19%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR----HRAEIDVARELALKLGARA 57
           K+ +V  SGG DS+        +  EV  +      R    H   +    ++A  L  + 
Sbjct: 4   KKVMVGMSGGIDSSVTAYMLQNEGYEVEGIYLKLHNRTDGYHEKNLGYIEDVAKFLNIKY 63

Query: 58  HKVLDVTLLN----ELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           + +      +    +  V S    + P P  +             R I F  +   +A Q
Sbjct: 64  YILDLADKFSKEVYDYFVDSYLTGTTPNPCVKC-----------NRQIKFGAM-LDFAKQ 111

Query: 114 VKAEAVITG-VCETDFSGYP---DCRDE---FVKALNH-AVSLGMAKDIRFETPLMWIDK 165
             A  + TG   +TD   +    D   +   F+  ++  A+   M        PL    K
Sbjct: 112 HGASYLATGHYAKTDGKFFYEADDKTKDQSYFLSQVDKDALKYMMF-------PLSTYKK 164

Query: 166 AETWALADYYGKLDLVRNE 184
            +         KLD+   +
Sbjct: 165 EDIVKFG---AKLDVAYKK 180


>UniRef50_B9B2T1 Putative uncharacterized protein n=2 Tax=Burkholderia
           RepID=B9B2T1_9BURK
          Length = 468

 Score = 51.0 bits (121), Expect = 4e-05,   Method: Composition-based stats.
 Identities = 24/117 (20%), Positives = 36/117 (30%), Gaps = 12/117 (10%)

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA-L 171
            +    ++TG  E        C   F   L+  V     K ++F  P ++  K      L
Sbjct: 252 SIGGSLIVTG-HEAKHRS---CYPGFTTKLSRFVEALTGKRVKFYHPALFETKGAVLRAL 307

Query: 172 ADYYGKLDLVRNETLTCY-------NGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           AD    +  V     +C           +   CG C  C LR    +    D  T  
Sbjct: 308 ADAGENVRAVLGRHRSCSCDARLTSRDEQMFHCGVCGNCLLRRTAEHSANLDGATEY 364


>UniRef50_C6HVS1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Leptospirillum ferrodiazotrophum RepID=C6HVS1_9BACT
          Length = 372

 Score = 50.6 bits (120), Expect = 4e-05,   Method: Composition-based stats.
 Identities = 35/208 (16%), Positives = 63/208 (30%), Gaps = 49/208 (23%)

Query: 1   MK--RAVVV-FSGGQDSTTCLVQALQQYDEVHCVTFD------------YGQRHRAEIDV 45
           MK  +++V+  SGG DS        ++  +V  V               +G+R    + +
Sbjct: 1   MKRNKSIVIGMSGGVDSAVSAWLLKKEGYDVTGVILRLWGKDSSEASKGWGERTCCHVPM 60

Query: 46  ARELALKLGARAHKVLDV-----TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRN 100
              L  K+    + ++D      + + +   S+      P P                  
Sbjct: 61  VEYLCQKILNIPYAIVDAESAFKSDVVDSFKSAYRTGETPNPCTSCNEK----------- 109

Query: 101 ILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEF-VKALNHAVSLGMAK------- 152
            + L L   +A +     V TG        Y    ++F V  L  A  L   +       
Sbjct: 110 -VKLPLLRRWAEENGIPKVATG-----HYTYWKRSEDFNVSGLAMAHDLAKDQSYFLSRT 163

Query: 153 ----DIRFETPLMWIDKAETWALADYYG 176
                     P+  + K +   LA   G
Sbjct: 164 PDLCPENTVFPVGRMSKPQVRELAREAG 191


>UniRef50_B6BIH0 tRNA(Ile)-lysidine synthetase n=1 Tax=Campylobacterales bacterium
          GD 1 RepID=B6BIH0_9PROT
          Length = 333

 Score = 50.6 bits (120), Expect = 4e-05,   Method: Composition-based stats.
 Identities = 19/74 (25%), Positives = 34/74 (45%), Gaps = 2/74 (2%)

Query: 2  KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR--AEIDVARELALKLGARAHK 59
          K+ ++ FSGG DST      L+Q  +      +Y +R +   E++ A+ELA K     H 
Sbjct: 13 KKNLLAFSGGADSTALFFLLLKQNIKFDIAIVNYNERVQSIKEVEYAKELASKYNLECHT 72

Query: 60 VLDVTLLNELAVSS 73
          ++   +       +
Sbjct: 73 LVSPKISKNFESKA 86


>UniRef50_D2RUT9 Argininosuccinate synthase n=1 Tax=Haloterrigena turkmenica DSM
          5511 RepID=D2RUT9_9EURY
          Length = 429

 Score = 50.6 bits (120), Expect = 5e-05,   Method: Composition-based stats.
 Identities = 26/64 (40%), Positives = 34/64 (53%), Gaps = 4/64 (6%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAH 58
          M R  + FSGG D+T C+    ++  YD+V  VT D GQ    E + A E A  LG   H
Sbjct: 1  MTRVALAFSGGLDTTVCVPLLEEEYGYDDVIGVTVDVGQPAS-EFEEAEETADALGLE-H 58

Query: 59 KVLD 62
           V+D
Sbjct: 59 YVVD 62


>UniRef50_C1XLL9 Asparagine synthase, glutamine-hydrolyzing n=1 Tax=Meiothermus
           ruber DSM 1279 RepID=C1XLL9_MEIRU
          Length = 611

 Score = 50.6 bits (120), Expect = 5e-05,   Method: Composition-based stats.
 Identities = 26/57 (45%), Positives = 31/57 (54%), Gaps = 4/57 (7%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYG--QRHRAEIDVARELALKLGARAHKV 60
           V  SGG DS+     A QQ++ V  V F YG  Q  R+E  +ARELA K G   H V
Sbjct: 273 VSLSGGLDSSLVAALAAQQHERV--VGFCYGHPQAPRSEGPLARELAQKAGIDVHFV 327


>UniRef50_Q64E49 PP-ATPase subunit of GMP synthase n=1 Tax=uncultured archaeon
           GZfos14B8 RepID=Q64E49_9ARCH
          Length = 310

 Score = 50.2 bits (119), Expect = 5e-05,   Method: Composition-based stats.
 Identities = 41/191 (21%), Positives = 62/191 (32%), Gaps = 34/191 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +AV+  SGG DST C V   +   D +  V  D G     E   A ++     AR     
Sbjct: 23  KAVIAVSGGVDSTVCAVLTHRAVGDRLVAVFIDDGLMREGE---AEQVMNFFKARNMNAR 79

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            V   NE            + D E +           RN  F ++ A    +  A  ++ 
Sbjct: 80  QVDAANEF-----FDALKGIVDPEEKRKA-------FRNT-FYSVLARVVREENANCLVQ 126

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI-------RFETPLMWIDKAETWALADY 174
           G    D +       E VK +    ++     I           PL  I K E   +A  
Sbjct: 127 GTIAADVA-------ETVKGIKTQHNILEQIGIDPGSYGLTIVEPLREIYKPEVREVARS 179

Query: 175 YGKLDLVRNET 185
           +G   +    +
Sbjct: 180 FG---VPPEFS 187


>UniRef50_D0NFB6 tRNA(Ile)-lysidine synthase, putative n=1 Tax=Phytophthora
           infestans T30-4 RepID=D0NFB6_PHYIN
          Length = 665

 Score = 50.2 bits (119), Expect = 5e-05,   Method: Composition-based stats.
 Identities = 40/187 (21%), Positives = 63/187 (33%), Gaps = 42/187 (22%)

Query: 6   VVFSGGQDSTTCLVQAL----QQYD-EVHCVTFDYGQR--HRAEIDVARELALKLGARAH 58
           V  SGG DS            +  D  +  V  DYG R    AE D  R    + G   H
Sbjct: 229 VSLSGGVDSMVVAYLMHKLKEKHGDFTIVAVHLDYGNRPESGAECDYVRRWCERFGIVFH 288

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            V  +  +      + TR      DYE  +  I  +               YA  ++   
Sbjct: 289 -VRRIDEVKR----ATTRRD----DYEKISREIRYS--------------TYAEVMQKYH 325

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSL---------GMAKDIRFETPLMWIDKAETW 169
           +  G+C     G  D ++  +  +   +SL          +   +R   PL+ ++K   +
Sbjct: 326 I-PGMCFGHHRG--DVQENVISNMMKGLSLLNLNGMQASSVVNGVRIWRPLLDLEKDVIF 382

Query: 170 ALADYYG 176
             A  YG
Sbjct: 383 EFAHRYG 389


>UniRef50_C7DGN0 Thiamine biosynthesis protein n=1 Tax=Candidatus Micrarchaeum
           acidiphilum ARMAN-2 RepID=C7DGN0_9EURY
          Length = 395

 Score = 50.2 bits (119), Expect = 5e-05,   Method: Composition-based stats.
 Identities = 39/186 (20%), Positives = 65/186 (34%), Gaps = 37/186 (19%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRH----RAEIDVARELALKLGARA 57
           RAVV+ SGG DS      A+++  +V  +    Y   H        ++  +LA   G   
Sbjct: 177 RAVVLLSGGIDSPVAAFYAMRRGFDVVYLHIHGYDDPHTAMASKMHELVGQLAKYSGKTR 236

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
                  +    A+ S        P YE          V  +  ++L L+   A ++  +
Sbjct: 237 VYYAPFHIFQMAAMGS-------APRYEL---------VLFKRFIYL-LSEKMADEMNID 279

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAK---DIRFETPLMWIDKAETWALADY 174
           A++TG      +                 +LG A+         PL+  DK E   +A  
Sbjct: 280 AIVTGESLGQVAS------------QTVSNLGSAQSGVRHLIFRPLIGFDKNEIIEMARR 327

Query: 175 YGKLDL 180
            G  D+
Sbjct: 328 IGTYDI 333


>UniRef50_Q2NEC4 Argininosuccinate synthase n=4 Tax=Methanobacteriaceae
          RepID=ASSY_METST
          Length = 392

 Score = 50.2 bits (119), Expect = 6e-05,   Method: Composition-based stats.
 Identities = 21/63 (33%), Positives = 33/63 (52%), Gaps = 2/63 (3%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQYD-EVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
          M++ V+ FSGG D++ C+    ++Y+ EV     D GQ    EI      A K+G+  H 
Sbjct: 1  MEKVVLAFSGGLDTSVCVKLLQEKYNMEVITACVDVGQPED-EIKKPEAAAQKIGSDKHY 59

Query: 60 VLD 62
           +D
Sbjct: 60 TID 62


>UniRef50_C7N321 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Slackia heliotrinireducens DSM 20476
           RepID=C7N321_SLAHD
          Length = 353

 Score = 49.8 bits (118), Expect = 6e-05,   Method: Composition-based stats.
 Identities = 49/201 (24%), Positives = 77/201 (38%), Gaps = 41/201 (20%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-Y------------GQRHRAEIDVAR 47
           ++R +V  SGG DS+      L+Q  EV   T + Y            G     +++ AR
Sbjct: 2   VQRVLVGMSGGVDSSVTAYLLLRQGYEVVGATMNLYDPGRLGVPDVRDG--ESNDLEDAR 59

Query: 48  ELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP----GRNILF 103
            +A +LG  AH VLD              +++  P  +  ADG+  T  P     R++ F
Sbjct: 60  SVANRLGI-AHHVLDFGCE--------FENNVVRPFAQAYADGL--TPNPCIACNRHLKF 108

Query: 104 -LTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLG-------MAKDIR 155
            L L A  A ++  + + TG      +   D R   +K L+ A             +   
Sbjct: 109 GLMLKA--ARELGCDGIATG-HYARIAQRLDGRFVLMKGLDDAKDQAYVLYHMTQDQLAH 165

Query: 156 FETPLMWIDKAETWALADYYG 176
              PL    K +  A+A   G
Sbjct: 166 TVLPLGEYTKQQVRAIAAEQG 186


>UniRef50_D1N6V4 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Victivallis vadensis ATCC BAA-548 RepID=D1N6V4_9BACT
          Length = 400

 Score = 49.8 bits (118), Expect = 6e-05,   Method: Composition-based stats.
 Identities = 39/185 (21%), Positives = 65/185 (35%), Gaps = 34/185 (18%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA------EIDVARELALKLGA- 55
           + + + SGG DS     Q +++      +TF     H A       +D  R +A  L   
Sbjct: 192 KVLTLLSGGIDSPVAAWQIMKRGCPTDFITF-----HSAPYTPPETVDKVRGIAEHLNTF 246

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
           + H VL +  L             P+     +        V  R    L +A   A    
Sbjct: 247 QQHGVLHLCNLA------------PMQKLIRDNCNERFRTVLYRRA-MLRIAEQVAKNTG 293

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
             A++TG      +       + +  +N    +  A D+    PL+  DK E+  +A   
Sbjct: 294 CRALVTGEAVGQVAS------QTIVNMNT---INRAVDMLVLRPLVGADKLESIRIAGEI 344

Query: 176 GKLDL 180
           G L+L
Sbjct: 345 GTLEL 349


>UniRef50_UPI000194D842 PREDICTED: argininosuccinate synthetase 1 n=5 Tax=Amniota
          RepID=UPI000194D842
          Length = 397

 Score = 49.8 bits (118), Expect = 6e-05,   Method: Composition-based stats.
 Identities = 22/64 (34%), Positives = 34/64 (53%), Gaps = 3/64 (4%)

Query: 2  KRAVVV-FSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
          K  VV+ +SGG D++  LV   +Q  +V     + GQ+   E   AR  AL LGA+   +
Sbjct: 5  KGTVVLAYSGGLDTSCILVWLKEQGYDVVAYLANIGQKEDFE--AARRKALALGAKKVYI 62

Query: 61 LDVT 64
           D++
Sbjct: 63 EDIS 66


>UniRef50_Q09580 Probable GMP synthase [glutamine-hydrolyzing] n=4 Tax=Chromadorea
           RepID=GUAA_CAEEL
          Length = 792

 Score = 49.8 bits (118), Expect = 7e-05,   Method: Composition-based stats.
 Identities = 17/61 (27%), Positives = 24/61 (39%), Gaps = 4/61 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYG-QRHRAEIDVARELALKLGARAH 58
           K+ +V+ SGG DS  C     +      V  +  D G  RH  E D   +    L    H
Sbjct: 320 KKVLVMVSGGVDSAVCAALLRRALGPNRVTAIHIDNGFMRHE-ESDAVEKSLAALDLPIH 378

Query: 59  K 59
           +
Sbjct: 379 R 379


>UniRef50_C5EME3 Asparagine synthase n=4 Tax=Bacteria RepID=C5EME3_9FIRM
          Length = 273

 Score = 49.8 bits (118), Expect = 7e-05,   Method: Composition-based stats.
 Identities = 41/175 (23%), Positives = 69/175 (39%), Gaps = 37/175 (21%)

Query: 8   FSGGQDSTTCLVQALQQ----YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           FSGG DS+  L  A         +V+ VTFD       ++++AR +A +LG   H+V++V
Sbjct: 25  FSGGVDSSLLLKVAADAAVETGKKVYAVTFDSRLHPSCDLEIARRVAGELGGI-HQVIEV 83

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
                            +   E   + +   ++  R+ LF++L    A +     ++ G 
Sbjct: 84  D---------------ELEQEEIRMNPVNRCYLCKRH-LFMSL-KKLAGEYGVSRILDGT 126

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL--MWIDKAETWALADYYG 176
            E D   Y   R   ++AL               +PL  + I K     +A  YG
Sbjct: 127 NEDDMHVY---RPG-IQALKEL---------GIISPLAELHITKDAVKQMAAEYG 168


>UniRef50_Q5LIS5 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Bacteroides
           RepID=MNMA1_BACFN
          Length = 364

 Score = 49.8 bits (118), Expect = 7e-05,   Method: Composition-based stats.
 Identities = 44/179 (24%), Positives = 69/179 (38%), Gaps = 27/179 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYGQRHRAE-IDVARELALKLGARAHK 59
           KR ++  SGG DS+   +  L+   EV  VTF  Y      E ++ AR LA +LG   H 
Sbjct: 6   KRVLLGMSGGTDSSVAAMLLLEAGYEVTGVTFRFYEFNGSTEYLEDARALAARLGI-GHI 64

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP---GRNILFLTLAAIYAYQVKA 116
             D   + +            + DY  +     +T VP     N L   L A  A ++  
Sbjct: 65  TYDARKVFQ----------EQIIDYFIDEYMSGHTPVPCTLCNNQLKWPLLAKIADEMGI 114

Query: 117 EAVITG------VCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAE 167
             + TG        + ++   P    E V         G+ ++I  R   P+  + K+E
Sbjct: 115 FYLATGHYVRKQWIDGNYYIAP---AEDVDKDQSFFLWGLRQEILQRMLLPMGGMTKSE 170


>UniRef50_A0LE34 Argininosuccinate synthase n=5 Tax=Bacteria RepID=ASSY_MAGSM
          Length = 407

 Score = 49.8 bits (118), Expect = 8e-05,   Method: Composition-based stats.
 Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 3/62 (4%)

Query: 3  RAVVVFSGGQDSTTCLVQALQQY-DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
          + V+ +SGG D++  L     +Y  EV     D GQ    E++ AR  A K G +   + 
Sbjct: 7  KVVLAYSGGLDTSIILKWLQDEYQCEVVAFCADLGQ--AEELEPARAKAEKFGVKEIYID 64

Query: 62 DV 63
          D+
Sbjct: 65 DL 66


>UniRef50_A6D100 Putative uncharacterized protein n=1 Tax=Vibrio shilonii AK1
           RepID=A6D100_9VIBR
          Length = 372

 Score = 49.8 bits (118), Expect = 8e-05,   Method: Composition-based stats.
 Identities = 43/263 (16%), Positives = 74/263 (28%), Gaps = 62/263 (23%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-----DYGQRHRAEIDVARELALKLGARA 57
           +AV + SGG DS    +  L    ++  +       D  Q ++ +++ A         + 
Sbjct: 47  KAVSLLSGGLDSFIGAIDLLNS--DIDTLFVGHYAKDGTQHYQNQVEQA--------LKG 96

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK-- 115
           H               L  D         E D   +     R+ +FL++AA  A  +   
Sbjct: 97  HYP---DYFKGYVKGCLIFDKSVFKGGNQEIDSENSQ--RSRSFMFLSMAAYLASSMGEL 151

Query: 116 AEAVI----------------TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETP 159
            + ++                 G   T           F+  +       +  +I    P
Sbjct: 152 CKLIVPENGFIALNVPLDPLRIGANSTKTVH-----PNFMSKMGEIFDF-LGLNIGLINP 205

Query: 160 LMWIDKAETWALADYYGKLDLVRNETLTCYNGFK------GDGCGH------------CA 201
              + K E  A       L    +ETL+C +  K      G                 C 
Sbjct: 206 YRHMTKGEMMANCKNKSLLTKHAHETLSCASQSKMRWDQGGFKANRLGGSGGNGNCGYCY 265

Query: 202 ACNLRANGLNHYLADKPTVMAAM 224
            C +R       L    T   A+
Sbjct: 266 PCLIRKASFQKALITDNTEYVAI 288


>UniRef50_D1J9C4 Putative uncharacterized protein n=1 Tax=uncultured archaeon
           RepID=D1J9C4_9ARCH
          Length = 711

 Score = 49.8 bits (118), Expect = 8e-05,   Method: Composition-based stats.
 Identities = 27/134 (20%), Positives = 53/134 (39%), Gaps = 16/134 (11%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDY---GQRHRAEIDVARELALKLGARAH 58
           +R  V+FSGG DST       +   +  C T      G +   ++D A+++A  LG +  
Sbjct: 232 ERFGVLFSGGLDSTLIAYLCKELGADFVCYTVAVEEPGMKDAEDLDYAKQIAADLGLKLK 291

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
               + +  ++    L      + D +     +          L L +A  +A     + 
Sbjct: 292 L---IKMRADVLEKPLHEVVSWIDDTDVVKVSV---------ALPLYVACEHARAEGIKT 339

Query: 119 VITGVCETD-FSGY 131
           ++ G+   + F+GY
Sbjct: 340 LLYGLGTEELFAGY 353


>UniRef50_Q8R5X3 tRNA-specific 2-thiouridylase mnmA 1 n=16 Tax=Fusobacterium
          RepID=MNMA1_FUSNN
          Length = 362

 Score = 49.8 bits (118), Expect = 8e-05,   Method: Composition-based stats.
 Identities = 20/62 (32%), Positives = 28/62 (45%), Gaps = 4/62 (6%)

Query: 3  RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
          R  V  SGG DS+T      QQ  ++  VT       + E   A+++   LG   H +LD
Sbjct: 25 RVGVAMSGGVDSSTVAYLLKQQGYDIFGVTMKT---FKDEDSDAKKVCDDLGIE-HYILD 80

Query: 63 VT 64
          V 
Sbjct: 81 VR 82


>UniRef50_A4X6Q9 Asparagine synthase (Glutamine-hydrolyzing) n=3
           Tax=Micromonosporaceae RepID=A4X6Q9_SALTO
          Length = 536

 Score = 49.8 bits (118), Expect = 8e-05,   Method: Composition-based stats.
 Identities = 20/60 (33%), Positives = 31/60 (51%), Gaps = 3/60 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
             VV SGG DS+  L+ A + + +   VT   G     ++  AR LA  LG   H+V+++
Sbjct: 230 VGVVLSGGLDSSLALLHARELHPDCVAVTV--GVPESPDLLYARRLAKDLGV-PHEVVEL 286


>UniRef50_A5GE36 tRNA-specific 2-thiouridylase mnmA n=20 Tax=Bacteria
           RepID=MNMA_GEOUR
          Length = 375

 Score = 49.4 bits (117), Expect = 9e-05,   Method: Composition-based stats.
 Identities = 45/198 (22%), Positives = 67/198 (33%), Gaps = 33/198 (16%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF---DYGQRHRAE-------------IDV 45
           KR V+  SGG DS+       ++  EV  ++    DY  R  AE             I  
Sbjct: 8   KRVVIAMSGGVDSSVAAALLKEEGHEVIGISMQVWDY-TRFTAEEGEKFDTCCSLDDIHD 66

Query: 46  ARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLT 105
           AR +A +L      V++     E   + +  D +     E      PN  V     +   
Sbjct: 67  ARRVAEQLEI-PFYVVN---FEEEFQNLVIDDFVN----EYLLGRTPNPCVRCNQWIKFE 118

Query: 106 LAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLG----MAKDIRF---ET 158
           L    A  + A+ + TG          D R   +K ++ A             +      
Sbjct: 119 LLLKKARGLGADMIATG-HYARTEQDADGRYRLMKGVDPAKDQSYFLFTLTQEQLAMTLF 177

Query: 159 PLMWIDKAETWALADYYG 176
           PL  + K E  ALA  YG
Sbjct: 178 PLGGMTKQEVRALAAGYG 195


>UniRef50_B1I677 tRNA-specific 2-thiouridylase mnmA n=1 Tax=Candidatus
          Desulforudis audaxviator MP104C RepID=MNMA_DESAP
          Length = 343

 Score = 49.4 bits (117), Expect = 9e-05,   Method: Composition-based stats.
 Identities = 22/68 (32%), Positives = 30/68 (44%), Gaps = 4/68 (5%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
          M    V  SGG DS+T  +   +    V  +T     R  AE   A  ++  LG   H V
Sbjct: 1  MATVAVALSGGVDSSTTALLMKEAGHRVFTLTMATNDRVAAE---AARVSAFLGL-PHHV 56

Query: 61 LDVTLLNE 68
          LD++ L E
Sbjct: 57 LDISGLFE 64


>UniRef50_C0GFP8 Asparagine synthase n=1 Tax=Dethiobacter alkaliphilus AHT 1
           RepID=C0GFP8_9FIRM
          Length = 483

 Score = 49.4 bits (117), Expect = 9e-05,   Method: Composition-based stats.
 Identities = 17/54 (31%), Positives = 28/54 (51%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           V  SGG DS+  L  A +   ++   T  + +RH  +++ A+ LA +LG   H 
Sbjct: 206 VALSGGLDSSIILAVAREFDKDIAAFTATFAERHGEDLEYAKLLADQLGVDHHI 259


>UniRef50_C9RAV2 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
          Tax=Ammonifex degensii KC4 RepID=C9RAV2_AMMDK
          Length = 344

 Score = 49.4 bits (117), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 25/65 (38%), Positives = 28/65 (43%), Gaps = 6/65 (9%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHK 59
          M R  V  SGG DS T     LQ   EV  VT    G     E + A E+A KL    H 
Sbjct: 1  MTRVAVALSGGVDSATAAFLLLQAGYEVFGVTMLVTG----EEGEKAAEVAAKLKI-PHF 55

Query: 60 VLDVT 64
          V D+ 
Sbjct: 56 VFDLR 60


>UniRef50_A9BIS3 tRNA-specific 2-thiouridylase mnmA n=1 Tax=Petrotoga mobilis SJ95
          RepID=MNMA_PETMO
          Length = 367

 Score = 49.4 bits (117), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 9/32 (28%), Positives = 16/32 (50%)

Query: 2  KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF 33
          K+ +++ SGG DS+       +Q   V  + F
Sbjct: 4  KKVLMLMSGGVDSSVAAYLLKEQNYHVIGLHF 35


>UniRef50_P59606 Argininosuccinate synthase n=22 Tax=Proteobacteria
          RepID=ASSY_XYLFT
          Length = 401

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 21/61 (34%), Positives = 30/61 (49%)

Query: 2  KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
          K  V+ FSGG D++ C+    +Q   VH V  D G     E D   + A +LG  +H  +
Sbjct: 4  KNIVLAFSGGLDTSFCIPYLKEQGYAVHTVFADTGGVDEDERDFIEKRAAELGVASHLTV 63

Query: 62 D 62
          D
Sbjct: 64 D 64


>UniRef50_A5UKF9 ATP-utilizing enzyme, PP-loop superfamily n=3
           Tax=Methanobrevibacter smithii RepID=A5UKF9_METS3
          Length = 336

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 36/190 (18%), Positives = 70/190 (36%), Gaps = 38/190 (20%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH---RAEIDVARELALKLGARAH 58
           ++  + FSGG DST     A +  D+   +T D    H      I   ++ A + G R H
Sbjct: 17  RKVAIAFSGGADSTLLAYLASEVSDDPIAITID---NHIFPTGFIKHCKKTAKEFGIR-H 72

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +++D+                        A+     FV  R++++  +   YA +   E+
Sbjct: 73  QIIDLNFY---------------EHENLLANKSDRCFV-CRDLMYGNI-KKYAEKNGYES 115

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           V+ G   +D     D R   +    +            E+P +   K  +  + +Y  + 
Sbjct: 116 VLDGNNISDLVL--D-RPGILITYKN----------NIESPFIHA-KLTSKEIHEYLNRH 161

Query: 179 DLVRNETLTC 188
            +  + + TC
Sbjct: 162 SIPFSRSTTC 171


>UniRef50_A8AAD7 PP-loop domain protein n=1 Tax=Ignicoccus hospitalis KIN4/I
           RepID=A8AAD7_IGNH4
          Length = 383

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 29/133 (21%), Positives = 49/133 (36%), Gaps = 22/133 (16%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD----YGQRHRAEIDVARELALKLGARA 57
           KR ++  SGG+DS   L    +   EV   T D    Y      E ++A+ +A +LG   
Sbjct: 47  KRVLLGLSGGKDSVALLYLLKKVGTEVVAATVDTVPNY---TSLEAELAKRVAEELGVTH 103

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           + V    L      ++L+   +        +          R+ L L      A ++  E
Sbjct: 104 YTVYAKEL---YGFTALSYKKLRRAPCSICSKLR-------RHALEL-----LARRLGIE 148

Query: 118 AVITGVCETDFSG 130
            + TG    D + 
Sbjct: 149 YIATGHNMDDMAS 161


>UniRef50_C7RPK0 Putative uncharacterized protein n=1 Tax=Candidatus Accumulibacter
           phosphatis clade IIA str. UW-1 RepID=C7RPK0_9PROT
          Length = 458

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 53/246 (21%), Positives = 83/246 (33%), Gaps = 42/246 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
            ++ +FSGG DS    +  LQ       ++      H  E   +     KL A+  K   
Sbjct: 150 DSLCLFSGGLDSLIGAINLLQAESAPLLIS------HFGESATSDAQ-NKLFAKLKKQYA 202

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPG----------RNILFLTLA--AIY 110
            +  + L V     D + V D   E      +F+            RN   L +    + 
Sbjct: 203 RSAFDRLRVGMTFDDGL-VKDVGSENSTRGRSFLFFALGVFAGTGLRNGFTLKVPENGLI 261

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAV-SLGMAKDIRFETPLMWIDKAETW 169
           A  V  + +  G   T  +        F+   N  +  LG+    R E P + + K E  
Sbjct: 262 ALNVPLDPLRLGSNSTRTTH-----PYFMARWNDLLDELGIDG--RVENPYLPMTKGEMV 314

Query: 170 ALADYYGKLDLVRN-ETLTC----------YNGFKGDGCGHCAACNLRANGLNHYLAD-- 216
            LA    KL L     +L+C          + G   + CG+C  C +R   LN    +  
Sbjct: 315 -LACANQKLLLELAPMSLSCAHPANARWQGHKGRGIEHCGYCLPCLIRRAALNRAWGEGG 373

Query: 217 KPTVMA 222
             T   
Sbjct: 374 DRTAYT 379


>UniRef50_B5YGH6 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
          Tax=Thermodesulfovibrio yellowstonii DSM 11347
          RepID=B5YGH6_THEYD
          Length = 342

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 16/62 (25%), Positives = 28/62 (45%), Gaps = 7/62 (11%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVT---FDYGQRHRAEIDVARELALKLGARA 57
          M++ ++  SGG DS+       ++   V  V    FD      A I++A++ A  L  + 
Sbjct: 1  MEKVLLAMSGGIDSSVAAYLLREKGMAVEGVFFILFD----EPANIELAKQTANFLNIKL 56

Query: 58 HK 59
          H 
Sbjct: 57 HI 58


>UniRef50_UPI0001C41AAB PP-loop family protein n=1 Tax=Methanobrevibacter ruminantium M1
           RepID=UPI0001C41AAB
          Length = 359

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 34/188 (18%), Positives = 70/188 (37%), Gaps = 34/188 (18%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           K+  + FS G DST     A +   +V  +T+D  Q      ++ A+  A +LG + H++
Sbjct: 18  KKIALAFSAGADSTLLAYLAKESDCDVLAITYD-NQIFPEGFLEFAKRRADELGIK-HEI 75

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++ + L  +   +       +         I                   A +   + +I
Sbjct: 76  IENSFL-MIYDVAHNTPQRCLTCRRLMYGSIKQV----------------ANERGYDIII 118

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G   TD     D R   +    +            E+PL+  +  E++ + D+  + D+
Sbjct: 119 DGNNITDLIH--D-RPGILMKYAN----------GIESPLIDAN-LESYEIHDFLEENDI 164

Query: 181 VRNETLTC 188
             +++ TC
Sbjct: 165 EYSKSTTC 172


>UniRef50_B3T2X9 Putative tRNA methyl transferase n=3 Tax=environmental samples
          RepID=B3T2X9_9ARCH
          Length = 268

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 17/60 (28%), Positives = 31/60 (51%), Gaps = 2/60 (3%)

Query: 4  AVVVFSGGQDSTTCLVQA-LQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +V  SGG DS      A  +       VT DY    + E++ A++++L++G + H V++
Sbjct: 19 VLVALSGGVDSALVAYSAYAKLGKSAISVTADYKTLAQEELEYAKKISLEIGIK-HIVIE 77


>UniRef50_A5FHA9 tRNA-specific 2-thiouridylase mnmA n=26 Tax=Bacteria
          RepID=MNMA_FLAJ1
          Length = 395

 Score = 49.1 bits (116), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 14/32 (43%), Positives = 16/32 (50%)

Query: 1  MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVT 32
          MKR VV  SGG DS+       QQ  EV  + 
Sbjct: 1  MKRVVVGLSGGVDSSVAAYLLQQQGYEVIGLF 32


>UniRef50_B1VAX7 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Bacteria
           RepID=MNMA_PHYAS
          Length = 375

 Score = 48.7 bits (115), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 39/207 (18%), Positives = 68/207 (32%), Gaps = 46/207 (22%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVT---------FDYGQRH-------RAEID 44
           MK+ VV  SGG DS    +   +   EV  V          FD+ Q +         E+D
Sbjct: 1   MKKVVVGLSGGVDSAVSALLLKKAGYEVEAVFMRNWDSQLNFDF-QGNPTLNDVCPQELD 59

Query: 45  V--ARELALKLGARAHKVLDVTLLNE----LAVSSLTRDSIPVPDYEPEADGIPNTFVPG 98
              A +++L+LG + H+V  +    +      +++   +  P PD     +         
Sbjct: 60  YKDALKVSLQLGIKLHRVNFIEEYWQKVFLYFINAFKNNLTPNPDILCNNEIKFKA---- 115

Query: 99  RNILFLTLAAI------YAYQVKAEAVITGVCETDFSGYPDCRDE---FVKALNHAVSLG 149
               F+  A         A    A  +     +   +   D   +   F+  L       
Sbjct: 116 ----FINYATSKLKPQYIAMGHYARLIYDKNQKVSLACPLDKNKDQTYFLSQLK------ 165

Query: 150 MAKDIRFETPLMWIDKAETWALADYYG 176
            ++      PL  + K E   +A   G
Sbjct: 166 TSQLKNILFPLADLTKKEVRKIALENG 192


>UniRef50_Q9YEW8 Probable tRNA sulfurtransferase n=1 Tax=Aeropyrum pernix
           RepID=THII_AERPE
          Length = 396

 Score = 48.7 bits (115), Expect = 1e-04,   Method: Composition-based stats.
 Identities = 37/185 (20%), Positives = 69/185 (37%), Gaps = 28/185 (15%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE--IDVARELALK--LGARAHK 59
           ++ ++SGG DS       +++   VH   +D+G     E  ++ A+ LA +   G R   
Sbjct: 181 SLALYSGGFDSGVAAWMIMRRGSPVHLAFYDFGVPEALEVAVEGAKTLAGEWAWGYRPRL 240

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
                 +     ++L  + +  P Y         T V  R  L L  A   A +   EA+
Sbjct: 241 -----YVVNFRGAALIVNGLVKPSYR--------TLVLRR--LMLLHAQDLAAREGFEAL 285

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +TG      +          + + +   +    ++    PL  +DK E    +   G  D
Sbjct: 286 VTGESVGQVAS---------QTVRNLRLISSGLELPVLRPLAGMDKDEIVEKSREIGLYD 336

Query: 180 LVRNE 184
           + R +
Sbjct: 337 IARRQ 341


Searching..................................................done


Results from round 3


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_UPI0001C171C7 ExsB n=2 Tax=Nostocaceae RepID=UPI0001C171C7   244   2e-63
UniRef50_B8E544 7-cyano-7-deazaguanine synthase n=198 Tax=Bacter...   244   2e-63
UniRef50_A4XFR8 7-cyano-7-deazaguanine synthase n=2 Tax=cellular...   239   4e-62
UniRef50_B9MMB0 7-cyano-7-deazaguanine synthase n=8 Tax=Bacteria...   238   8e-62
UniRef50_Q1LTK2 7-cyano-7-deazaguanine synthase n=234 Tax=Bacter...   236   3e-61
UniRef50_B2KDX5 ExsB protein n=1 Tax=Elusimicrobium minutum Pei1...   236   4e-61
UniRef50_A2STX4 7-cyano-7-deazaguanine synthase n=3 Tax=cellular...   236   6e-61
UniRef50_A0LJR5 7-cyano-7-deazaguanine synthase n=4 Tax=Bacteria...   234   2e-60
UniRef50_B8GJL3 7-cyano-7-deazaguanine synthase n=6 Tax=Euryarch...   233   3e-60
UniRef50_A3ZMY4 Predicted PP-loop superfamily ATPase n=1 Tax=Bla...   233   6e-60
UniRef50_Q8YM45 7-cyano-7-deazaguanine synthase n=14 Tax=cellula...   231   1e-59
UniRef50_A8EWF5 7-cyano-7-deazaguanine synthase n=27 Tax=Epsilon...   230   3e-59
UniRef50_A8GPK3 7-cyano-7-deazaguanine synthase n=18 Tax=Proteob...   229   5e-59
UniRef50_B2UN88 ExsB protein n=6 Tax=Bacteria RepID=B2UN88_AKKM8      229   6e-59
UniRef50_B2I4Y4 7-cyano-7-deazaguanine synthase n=16 Tax=Bacteri...   229   7e-59
UniRef50_B8HQ99 7-cyano-7-deazaguanine synthase n=1 Tax=Cyanothe...   227   2e-58
UniRef50_O29807 7-cyano-7-deazaguanine synthase n=1 Tax=Archaeog...   227   3e-58
UniRef50_Q1QVW9 7-cyano-7-deazaguanine synthase n=23 Tax=Proteob...   226   5e-58
UniRef50_Q55468 7-cyano-7-deazaguanine synthase n=43 Tax=Bacteri...   225   1e-57
UniRef50_C8R2H0 ExsB protein n=1 Tax=Desulfurivibrio alkaliphilu...   225   1e-57
UniRef50_B3QPB3 7-cyano-7-deazaguanine synthase n=4 Tax=cellular...   224   1e-57
UniRef50_B0S9S9 7-cyano-7-deazaguanine synthase n=13 Tax=Bacteri...   224   2e-57
UniRef50_A7HHA2 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria...   224   3e-57
UniRef50_C1D6L0 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria...   224   3e-57
UniRef50_B9Z9V1 ExsB protein n=2 Tax=Halobacteriaceae RepID=B9Z9...   223   4e-57
UniRef50_Q8K986 7-cyano-7-deazaguanine synthase n=1 Tax=Buchnera...   222   7e-57
UniRef50_Q1QCP3 7-cyano-7-deazaguanine synthase n=122 Tax=Bacter...   222   7e-57
UniRef50_C1YMB1 PreQ(0) biosynthesis protein QueC n=1 Tax=Nocard...   221   2e-56
UniRef50_B2RJS2 7-cyano-7-deazaguanine synthase n=5 Tax=Bacteria...   220   3e-56
UniRef50_C0QST3 7-cyano-7-deazaguanine synthase n=7 Tax=Aquifica...   218   1e-55
UniRef50_Q2N612 7-cyano-7-deazaguanine synthase n=56 Tax=Bacteri...   218   1e-55
UniRef50_B6CWJ6 ToyM n=3 Tax=Bacteria RepID=B6CWJ6_STRRM              217   2e-55
UniRef50_A0B6A6 7-cyano-7-deazaguanine synthase n=4 Tax=Archaea ...   216   7e-55
UniRef50_Q3AR02 7-cyano-7-deazaguanine synthase n=12 Tax=Bacteri...   216   7e-55
UniRef50_D1PYH8 Transcription regulator ExsB n=5 Tax=Prevotella ...   215   8e-55
UniRef50_Q6ARX9 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria...   214   2e-54
UniRef50_Q12WI3 7-cyano-7-deazaguanine synthase n=6 Tax=cellular...   213   3e-54
UniRef50_C0CIL2 Putative uncharacterized protein n=1 Tax=Blautia...   213   4e-54
UniRef50_C4Z7K4 Queuosine biosynthesis protein QueC n=5 Tax=Bact...   212   9e-54
UniRef50_A5ULR3 7-cyano-7-deazaguanine synthase n=5 Tax=Methanob...   211   2e-53
UniRef50_Q1IHK6 7-cyano-7-deazaguanine synthase n=9 Tax=Bacteria...   211   2e-53
UniRef50_A6UV33 7-cyano-7-deazaguanine synthase n=12 Tax=Methano...   209   5e-53
UniRef50_Q1CTN3 7-cyano-7-deazaguanine synthase n=12 Tax=Helicob...   208   1e-52
UniRef50_D1R557 Putative uncharacterized protein n=1 Tax=Parachl...   208   1e-52
UniRef50_A6DLD5 ExsB protein n=1 Tax=Lentisphaera araneosa HTCC2...   207   2e-52
UniRef50_Q7NCE2 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria...   207   3e-52
UniRef50_UPI0001AF28A8 succinoglycan biosynthesis regulator n=2 ...   206   6e-52
UniRef50_A6VQW6 7-cyano-7-deazaguanine synthase n=22 Tax=Pasteur...   205   8e-52
UniRef50_UPI0001C418E0 queuosine biosynthesis protein QueC n=1 T...   205   1e-51
UniRef50_A5A3S4 BcepGomrgp35 n=1 Tax=Burkholderia phage BcepGomr...   205   1e-51
UniRef50_A6LIS9 7-cyano-7-deazaguanine synthase n=88 Tax=Bacteri...   204   1e-51
UniRef50_Q7M8H4 7-cyano-7-deazaguanine synthase n=2 Tax=Campylob...   204   2e-51
UniRef50_Q857D1 Gp3 n=2 Tax=unclassified Siphoviridae RepID=Q857...   204   2e-51
UniRef50_D0MDN1 ExsB protein n=1 Tax=Rhodothermus marinus DSM 42...   203   4e-51
UniRef50_B0TFM0 Exsb protein, putative n=1 Tax=Heliobacterium mo...   202   6e-51
UniRef50_D2RGV6 ExsB protein n=2 Tax=Archaeoglobaceae RepID=D2RG...   202   7e-51
UniRef50_B9L187 ExsB protein n=1 Tax=Thermomicrobium roseum DSM ...   202   1e-50
UniRef50_Q981C9 7-cyano-7-deazaguanine synthase n=13 Tax=Thermop...   200   2e-50
UniRef50_Q8UAM7 7-cyano-7-deazaguanine synthase n=71 Tax=Alphapr...   199   6e-50
UniRef50_Q5JHU5 7-cyano-7-deazaguanine synthase n=6 Tax=Thermoco...   199   6e-50
UniRef50_A9A531 ExsB family protein n=2 Tax=Thaumarchaeota RepID...   197   2e-49
UniRef50_C7ML00 ExsB protein n=9 Tax=Bacteria RepID=C7ML00_CRYCD      196   6e-49
UniRef50_D2RLP8 ExsB protein n=3 Tax=Bacteria RepID=D2RLP8_ACIFE      194   2e-48
UniRef50_Q8U4N3 7-cyano-7-deazaguanine synthase n=3 Tax=Pyrococc...   193   3e-48
UniRef50_B3T674 Putative ExsB n=1 Tax=uncultured marine crenarch...   191   1e-47
UniRef50_C0WAE7 Archaeosine biosynthesis protein queC n=3 Tax=Ba...   191   2e-47
UniRef50_Q0W7U0 Succinoglycan biosynthesis regulator n=1 Tax=unc...   191   2e-47
UniRef50_Q6WI28 ExsB protein n=1 Tax=Vibrio phage KVP40 RepID=Q6...   191   2e-47
UniRef50_C6XGK1 ExsB protein n=9 Tax=Bacteria RepID=C6XGK1_LIBAP      190   3e-47
UniRef50_B4E7W5 7-cyano-7-deazaguanine synthase n=73 Tax=Bacteri...   189   7e-47
UniRef50_Q8TWG9 7-cyano-7-deazaguanine synthase n=1 Tax=Methanop...   187   2e-46
UniRef50_A8AAU7 ExsB protein n=1 Tax=Ignicoccus hospitalis KIN4/...   186   6e-46
UniRef50_C3XFP7 Queuosine biosynthesis protein queC n=1 Tax=Heli...   185   1e-45
UniRef50_B3G2Q8 Putative uncharacterized protein n=1 Tax=Pseudom...   182   8e-45
UniRef50_Q2IUE0 7-cyano-7-deazaguanine synthase 2 n=1 Tax=Rhodop...   181   1e-44
UniRef50_D1R584 Putative uncharacterized protein n=1 Tax=Parachl...   179   5e-44
UniRef50_Q0BQ87 7-cyano-7-deazaguanine synthase n=76 Tax=Bacteri...   179   7e-44
UniRef50_Q6MKZ5 7-cyano-7-deazaguanine synthase n=1 Tax=Bdellovi...   178   2e-43
UniRef50_A4J5C7 7-cyano-7-deazaguanine synthase n=4 Tax=Clostrid...   174   3e-42
UniRef50_UPI00016988F1 exsB protein n=1 Tax=Endoriftia persephon...   167   2e-40
UniRef50_B8GP80 ExsB family protein n=1 Tax=Thioalkalivibrio sp....   164   2e-39
UniRef50_D2R400 ExsB family protein n=1 Tax=Pirellula staleyi DS...   160   3e-38
UniRef50_B9ZKA9 ExsB family protein n=1 Tax=Thioalkalivibrio sp....   159   6e-38
UniRef50_Q96ZD9 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Sulfol...   158   1e-37
UniRef50_B7GTM4 ExsB family protein n=2 Tax=Actinobacteridae Rep...   156   4e-37
UniRef50_A8URJ3 Putative uncharacterized protein n=1 Tax=Hydroge...   156   5e-37
UniRef50_Q8ZVX7 7-cyano-7-deazaguanine synthase n=7 Tax=Thermopr...   155   1e-36
UniRef50_B9M1I5 ExsB family protein n=4 Tax=Proteobacteria RepID...   154   3e-36
UniRef50_A9U3Q1 Predicted protein n=1 Tax=Physcomitrella patens ...   153   5e-36
UniRef50_B1XTB6 ExsB protein n=1 Tax=Polynucleobacter necessariu...   150   4e-35
UniRef50_D2PHM6 ExsB family protein n=3 Tax=Sulfolobus islandicu...   149   8e-35
UniRef50_Q24ZT5 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Desulf...   148   2e-34
UniRef50_C8PJP9 Queuosine biosynthesis protein QueC n=1 Tax=Camp...   147   2e-34
UniRef50_Q13AQ3 ExsB n=6 Tax=Proteobacteria RepID=Q13AQ3_RHOPS        147   4e-34
UniRef50_A4YH21 ExsB family protein n=1 Tax=Metallosphaera sedul...   146   6e-34
UniRef50_UPI00016C5260 ExsB n=1 Tax=Gemmata obscuriglobus UQM 22...   142   1e-32
UniRef50_D2L9Z8 ExsB family protein n=1 Tax=Desulfovibrio sp. FW...   140   3e-32
UniRef50_A8V022 Trans-regulatory protein ExsB n=1 Tax=Hydrogeniv...   139   5e-32
UniRef50_Q12C85 ExsB n=1 Tax=Polaromonas sp. JS666 RepID=Q12C85_...   139   9e-32
UniRef50_Q989H2 Mll6423 protein n=2 Tax=Proteobacteria RepID=Q98...   137   2e-31
UniRef50_C7RWC1 ExsB family protein n=4 Tax=Proteobacteria RepID...   132   1e-29
UniRef50_C9Q119 Putative uncharacterized protein n=1 Tax=Prevote...   131   2e-29
UniRef50_C3NEW9 ExsB family protein n=5 Tax=Sulfolobus RepID=C3N...   130   3e-29
UniRef50_B9LWH7 ExsB family protein n=1 Tax=Halorubrum lacusprof...   128   2e-28
UniRef50_Q9RZH7 Putative uncharacterized protein n=1 Tax=Deinoco...   125   9e-28
UniRef50_Q2RGB7 ExsB n=1 Tax=Moorella thermoacetica ATCC 39073 R...   124   2e-27
UniRef50_B1ZBL1 ExsB family protein n=4 Tax=Proteobacteria RepID...   124   2e-27
UniRef50_Q0BZ62 Putative uncharacterized protein n=1 Tax=Hyphomo...   121   2e-26
UniRef50_Q3JDB4 Putative uncharacterized protein n=1 Tax=Nitroso...   120   3e-26
UniRef50_C6ALB1 Putative uncharacterized protein n=2 Tax=Proteob...   120   5e-26
UniRef50_B8JF98 PP-loop superfamily ATPase-like protein n=1 Tax=...   119   8e-26
UniRef50_B0V938 Putative uncharacterized protein n=4 Tax=Acineto...   119   1e-25
UniRef50_A9GG06 Putative uncharacterized protein n=1 Tax=Sorangi...   117   2e-25
UniRef50_Q5WAJ2 Putative uncharacterized protein n=4 Tax=Bacillu...   116   7e-25
UniRef50_Q4UTY2 Putative uncharacterized protein n=2 Tax=Gammapr...   116   7e-25
UniRef50_B1M991 Putative uncharacterized protein n=2 Tax=Proteob...   116   7e-25
UniRef50_C6NX81 Archaeosine biosynthesis protein queC n=1 Tax=Ac...   114   2e-24
UniRef50_Q8R5Z5 tRNA-specific 2-thiouridylase mnmA 2 n=12 Tax=Fu...   112   1e-23
UniRef50_B5XVJ1 Putative uncharacterized protein n=1 Tax=Klebsie...   112   1e-23
UniRef50_C5PNW4 ExsB superfamily protein n=1 Tax=Sphingobacteriu...   111   2e-23
UniRef50_C7X359 Putative uncharacterized protein n=1 Tax=Enteroc...   110   4e-23
UniRef50_Q1LLD1 Putative uncharacterized protein n=1 Tax=Cupriav...   108   1e-22
UniRef50_A1SFM8 ExsB family protein n=1 Tax=Nocardioides sp. JS6...   108   2e-22
UniRef50_A8L9C0 ExsB family protein n=2 Tax=Actinomycetales RepI...   107   3e-22
UniRef50_A0B2R8 Putative uncharacterized protein n=4 Tax=Burkhol...   107   3e-22
UniRef50_Q12C76 Putative uncharacterized protein n=1 Tax=Polarom...   107   4e-22
UniRef50_A8AM74 Putative uncharacterized protein n=8 Tax=Gammapr...   107   5e-22
UniRef50_B4U7S9 ExsB family protein n=1 Tax=Hydrogenobaculum sp....   106   6e-22
UniRef50_A6D100 Putative uncharacterized protein n=1 Tax=Vibrio ...   105   1e-21
UniRef50_A6Q239 tRNA-specific 2-thiouridylase mnmA n=2 Tax=Epsil...   104   3e-21
UniRef50_Q9UWQ2 Putative thiamin biosynthesis protein thiI n=1 T...   104   3e-21
UniRef50_Q0AM89 Putative uncharacterized protein n=1 Tax=Maricau...   103   5e-21
UniRef50_A4T1U6 Putative uncharacterized protein n=2 Tax=Actinom...   102   1e-20
UniRef50_B5YCZ9 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...   102   1e-20
UniRef50_Q5JCY1 Probable tRNA sulfurtransferase n=14 Tax=Thermoc...   101   2e-20
UniRef50_A5GE36 tRNA-specific 2-thiouridylase mnmA n=20 Tax=Bact...   100   3e-20
UniRef50_B2IBH3 tRNA-specific 2-thiouridylase mnmA n=141 Tax=roo...   100   3e-20
UniRef50_B5YGH6 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...   100   4e-20
UniRef50_C7N321 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...   100   7e-20
UniRef50_A0YNH9 Putative uncharacterized protein n=2 Tax=Oscilla...    99   8e-20
UniRef50_C4ZB38 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    99   1e-19
UniRef50_C6MK22 Putative uncharacterized protein n=2 Tax=Deltapr...    99   2e-19
UniRef50_Q9V1S6 ThiI thiamin biosynthesis protein thiI n=3 Tax=P...    98   2e-19
UniRef50_A0PSW2 Putative uncharacterized protein n=1 Tax=Mycobac...    98   2e-19
UniRef50_C7RPK0 Putative uncharacterized protein n=1 Tax=Candida...    98   3e-19
UniRef50_Q72HP1 Probable tRNA sulfurtransferase n=3 Tax=Thermus ...    98   3e-19
UniRef50_C9RQ01 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    97   4e-19
UniRef50_UPI0001B54B61 PP-loop superfamily ATPase-like protein n...    97   4e-19
UniRef50_C0B5F7 Putative uncharacterized protein n=2 Tax=Lachnos...    96   8e-19
UniRef50_D0XY41 Putative uncharacterized protein n=1 Tax=Cauloba...    96   1e-18
UniRef50_UPI00016C4B95 tRNA (5-methylaminomethyl-2-thiouridylate...    96   1e-18
UniRef50_B0TFA7 tRNA-specific 2-thiouridylase mnmA n=97 Tax=Bact...    95   2e-18
UniRef50_D0X1F3 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    95   2e-18
UniRef50_B9JNY0 Putative uncharacterized protein n=1 Tax=Agrobac...    95   2e-18
UniRef50_B1VAX7 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Bacte...    94   3e-18
UniRef50_O29382 Probable tRNA sulfurtransferase n=2 Tax=Archaeog...    94   3e-18
UniRef50_Q5LIS5 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Bac...    94   4e-18
UniRef50_B9L3Q7 Thiamine biosynthesis/tRNA modification protein ...    94   4e-18
UniRef50_Q8A2F1 tRNA-specific 2-thiouridylase mnmA 2 n=8 Tax=Bac...    93   9e-18
UniRef50_C5CE34 Thiamine biosynthesis/tRNA modification protein ...    93   9e-18
UniRef50_B6YWN1 ThiI-related protein n=5 Tax=Euryarchaeota RepID...    92   9e-18
UniRef50_Q5P5F5 Putative uncharacterized protein n=1 Tax=Aromato...    92   1e-17
UniRef50_O67015 Putative uncharacterized protein n=1 Tax=Aquifex...    92   1e-17
UniRef50_C4DG87 Predicted PP-loop superfamily ATPase n=1 Tax=Sta...    92   1e-17
UniRef50_UPI0001BC5A6B tRNA (5-methylaminomethyl-2-thiouridylate...    92   2e-17
UniRef50_B7J5X6 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Acidi...    92   2e-17
UniRef50_A6LSF2 tRNA-specific 2-thiouridylase mnmA n=19 Tax=Clos...    91   2e-17
UniRef50_A5FHA9 tRNA-specific 2-thiouridylase mnmA n=26 Tax=Bact...    91   3e-17
UniRef50_Q73PV6 tRNA-specific 2-thiouridylase mnmA n=2 Tax=Trepo...    91   3e-17
UniRef50_A7ZEK1 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Campy...    91   3e-17
UniRef50_UPI000197B781 hypothetical protein BACCOPRO_02286 n=1 T...    90   4e-17
UniRef50_C7M0G9 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl...    90   4e-17
UniRef50_B9QRC5 Putative uncharacterized protein n=1 Tax=Labrenz...    90   4e-17
UniRef50_A1STI9 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Prote...    90   5e-17
UniRef50_C9RAV2 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    90   6e-17
UniRef50_Q2LWA6 tRNA-specific 2-thiouridylase mnmA 1 n=1 Tax=Syn...    90   6e-17
UniRef50_B5CVE8 Putative uncharacterized protein n=1 Tax=Bactero...    90   7e-17
UniRef50_C9L0F1 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    90   8e-17
UniRef50_B2A8M8 Probable tRNA sulfurtransferase n=2 Tax=Clostrid...    89   8e-17
UniRef50_A6KZV1 tRNA-specific 2-thiouridylase mnmA 3 n=23 Tax=Ba...    89   9e-17
UniRef50_A3CVZ1 ExsB family protein n=1 Tax=Methanoculleus maris...    89   1e-16
UniRef50_B4D711 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    89   1e-16
UniRef50_A8WT10 Putative uncharacterized protein n=2 Tax=Caenorh...    88   2e-16
UniRef50_C7DGN0 Thiamine biosynthesis protein n=1 Tax=Candidatus...    88   2e-16
UniRef50_Q2RZN9 tRNA-specific 2-thiouridylase mnmA n=11 Tax=Bact...    88   3e-16
UniRef50_A3DEB6 Probable tRNA sulfurtransferase n=34 Tax=Bacteri...    88   3e-16
UniRef50_Q3SW10 Putative uncharacterized protein n=1 Tax=Nitroba...    88   3e-16
UniRef50_UPI000069DE11 tRNA (5-methylaminomethyl-2-thiouridylate...    87   3e-16
UniRef50_Q0C5H6 Putative uncharacterized protein n=1 Tax=Hyphomo...    87   4e-16
UniRef50_A8ES35 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Eps...    87   4e-16
UniRef50_A5INK8 ExsB family protein n=6 Tax=Thermotogaceae RepID...    87   5e-16
UniRef50_D1N0N9 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    87   5e-16
UniRef50_Q7URE0 Putative uncharacterized protein n=1 Tax=Rhodopi...    87   5e-16
UniRef50_A4RU81 Predicted protein n=3 Tax=Ostreococcus RepID=A4R...    87   6e-16
UniRef50_C6J374 Thiamine biosynthesis/tRNA modification protein ...    87   7e-16
UniRef50_Q9ZJQ0 tRNA-specific 2-thiouridylase mnmA n=22 Tax=Camp...    86   7e-16
UniRef50_A6LQW7 ExsB family protein n=4 Tax=Clostridium RepID=A6...    86   7e-16
UniRef50_B8D4I6 tRNA sulfurtransferase n=1 Tax=Desulfurococcus k...    86   7e-16
UniRef50_Q7U320 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Helic...    86   8e-16
UniRef50_Q8TWX3 ATP pyrophosphatase involved in thiamine biosynt...    86   8e-16
UniRef50_Q13XN6 Putative uncharacterized protein n=1 Tax=Burkhol...    86   9e-16
UniRef50_Q0A9U9 Thiamine biosynthesis/tRNA modification protein ...    86   9e-16
UniRef50_A1U6U9 tRNA sulfurtransferase n=11 Tax=Gammaproteobacte...    85   1e-15
UniRef50_Q9Y933 GMP synthase [glutamine-hydrolyzing] n=5 Tax=Arc...    85   2e-15
UniRef50_D0LKW7 Queuosine synthesis-like protein n=1 Tax=Haliang...    85   2e-15
UniRef50_A6KZ28 tRNA-specific 2-thiouridylase mnmA 2 n=10 Tax=Ba...    85   2e-15
UniRef50_D1N6V4 Thiamine biosynthesis/tRNA modification protein ...    85   2e-15
UniRef50_Q17440 Probable mitochondrial tRNA-specific 2-thiouridy...    85   2e-15
UniRef50_Q09580 Probable GMP synthase [glutamine-hydrolyzing] n=...    85   2e-15
UniRef50_Q65G52 Probable tRNA sulfurtransferase n=20 Tax=Bacilla...    85   3e-15
UniRef50_C8WW19 Thiamine biosynthesis/tRNA modification protein ...    84   3e-15
UniRef50_B9B2T1 Putative uncharacterized protein n=2 Tax=Burkhol...    84   3e-15

Sequences not found previously or not previously below threshold:

UniRef50_A3W978 Putative uncharacterized protein n=2 Tax=Rhodoba...    96   1e-18
UniRef50_B7PXE7 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    93   9e-18
UniRef50_C9KPU1 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    92   9e-18
UniRef50_C7P7P9 Thiamine biosynthesis/tRNA modification protein ...    92   1e-17
UniRef50_A7I9U0 Thiamine biosynthesis protein n=2 Tax=Genera inc...    92   2e-17
UniRef50_B1L665 Thiamine biosynthesis/tRNA modification protein ...    92   2e-17
UniRef50_A8DJE2 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    91   2e-17
UniRef50_B4R9S2 Putative uncharacterized protein n=1 Tax=Phenylo...    91   2e-17
UniRef50_O27720 Probable tRNA sulfurtransferase n=5 Tax=Methanob...    91   2e-17
UniRef50_B0K991 tRNA-specific 2-thiouridylase mnmA 2 n=9 Tax=Clo...    91   3e-17
UniRef50_A6W0E1 tRNA-specific 2-thiouridylase mnmA n=20 Tax=Bact...    90   4e-17
UniRef50_Q2FLN6 Thiamine biosynthesis protein n=1 Tax=Methanospi...    90   4e-17
UniRef50_Q58341 Probable tRNA sulfurtransferase n=11 Tax=Methano...    90   5e-17
UniRef50_Q3DBK5 TRNA methyl transferase (Fragment) n=5 Tax=cellu...    90   5e-17
UniRef50_A5UV64 tRNA-specific 2-thiouridylase mnmA n=7 Tax=Bacte...    90   6e-17
UniRef50_C7NES3 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl...    88   2e-16
UniRef50_D0LWB4 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl...    87   4e-16
UniRef50_Q47DY3 Putative uncharacterized protein n=5 Tax=Proteob...    87   5e-16
UniRef50_C0E8G4 Putative uncharacterized protein n=1 Tax=Clostri...    87   5e-16
UniRef50_C2KV29 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    87   6e-16
UniRef50_A7I2L9 tRNA-specific 2-thiouridylase mnmA n=19 Tax=Camp...    87   6e-16
UniRef50_B5DNY9 GA22395 n=14 Tax=Neoptera RepID=B5DNY9_DROPS           86   8e-16
UniRef50_D0WIU4 tRNA (5-methylaminomethyl-2-thiouridylate)-methy...    86   8e-16
UniRef50_C1A7G2 tRNA-specific 2-thiouridylase MnmA n=1 Tax=Gemma...    86   9e-16
UniRef50_D1BMF0 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl...    86   9e-16
UniRef50_Q64DI2 Thiamine biosynthesis ATP pyrophosphatase n=3 Ta...    86   9e-16
UniRef50_A2PQ54 Putative uncharacterized protein n=1 Tax=Vibrio ...    86   1e-15
UniRef50_B1YTE9 tRNA-specific 2-thiouridylase mnmA n=310 Tax=cel...    86   1e-15
UniRef50_Q9X220 Probable tRNA sulfurtransferase n=6 Tax=Thermoto...    86   1e-15
UniRef50_B1ZZ32 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Verru...    85   1e-15
UniRef50_B3DUT9 tRNA(5-methylaminomethyl-2-thiouridine)-methyltr...    85   1e-15
UniRef50_Q5NVV1 Thiamine biosynthesis protein (ATP pyrophosphata...    85   2e-15
UniRef50_C6D5K7 Thiamine biosynthesis/tRNA modification protein ...    85   2e-15
UniRef50_B3JKC8 Putative uncharacterized protein n=2 Tax=Bactero...    85   2e-15
UniRef50_A6TQ96 Probable tRNA sulfurtransferase n=14 Tax=Clostri...    85   2e-15
UniRef50_D2MP56 Thiamine biosynthesis/tRNA modification protein ...    84   3e-15
UniRef50_Q1NYA2 TRNA (5-methylaminomethyl-2-thiouridylate)-methy...    84   3e-15
UniRef50_Q1AWB1 tRNA-specific 2-thiouridylase mnmA n=1 Tax=Rubro...    84   3e-15

>UniRef50_UPI0001C171C7 ExsB n=2 Tax=Nostocaceae RepID=UPI0001C171C7
          Length = 282

 Score =  244 bits (622), Expect = 2e-63,   Method: Composition-based stats.
 Identities = 81/232 (34%), Positives = 118/232 (50%), Gaps = 13/232 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + +AV++ SGG DS+T L QA     E + ++FDY QRHR E+  A  +A  L    H++
Sbjct: 56  IMKAVILLSGGLDSSTVLYQARADGYECYVISFDYHQRHRRELQSAISIAQSLEVVEHQL 115

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +    L     S+LT +SIPVP   P    +  IP T+VP RN +FL+ A  YA  ++AE
Sbjct: 116 VTFD-LRSWGGSALTDNSIPVPQNRPLDQMSGSIPITYVPARNTIFLSFALAYAEAIRAE 174

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALA 172
            V  GV   D+SGYPDCR +++ A+     LG       K I    PL+ + K++   L 
Sbjct: 175 RVYIGVNALDYSGYPDCRPDYIHAMQEVFRLGTKQGREGKRIDIVAPLINLTKSQIIQLG 234

Query: 173 DYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
                L +    T +CY+G +   CG C +C LR    +    + P     M
Sbjct: 235 ---NLLGVPWELTWSCYSGDQ-QPCGVCDSCQLRWAAFHELGLEDPVKQQGM 282


>UniRef50_B8E544 7-cyano-7-deazaguanine synthase n=198 Tax=Bacteria RepID=QUEC_SHEB2
          Length = 240

 Score =  244 bits (622), Expect = 2e-63,   Method: Composition-based stats.
 Identities = 154/227 (67%), Positives = 186/227 (81%), Gaps = 1/227 (0%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AVVVFSGGQDSTTCL+QAL QYDEVH +TFDYGQRHR EI+VA+ LA +L   +HKV+D
Sbjct: 13  KAVVVFSGGQDSTTCLIQALTQYDEVHGITFDYGQRHREEIEVAKSLAKRLKITSHKVMD 72

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           VTLLNELA+S+LTRD+IPV  +E   +G+PNTFVPGRNILFLTLA IYAYQ+ AEA+ITG
Sbjct: 73  VTLLNELAISALTRDAIPV-SHELMENGLPNTFVPGRNILFLTLAGIYAYQLGAEAIITG 131

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           VCETDFSGYPDCR++FVKA+  A+  GM K ++  TPLMW++KA+TWALAD Y +LDLVR
Sbjct: 132 VCETDFSGYPDCRNDFVKAMESALVQGMDKQLKIITPLMWLNKAQTWALADKYQQLDLVR 191

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTG 229
           + TLTCYNG  GDGCG C AC+LR  GL+ Y+ DK  VMA++     
Sbjct: 192 HHTLTCYNGVIGDGCGDCPACHLRKRGLDEYMQDKTAVMASLDASEP 238


>UniRef50_A4XFR8 7-cyano-7-deazaguanine synthase n=2 Tax=cellular organisms
           RepID=QUEC_CALS8
          Length = 225

 Score =  239 bits (611), Expect = 4e-62,   Method: Composition-based stats.
 Identities = 74/219 (33%), Positives = 112/219 (51%), Gaps = 5/219 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVVV SGG DSTT L     Q  E + ++F YGQ+H  E++ A++    L    HK++D
Sbjct: 2   RAVVVLSGGMDSTTLLYDVKNQGYETYTISFLYGQKHSKELEFAKKTCELLKV-PHKIVD 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++   +LA S+LT+ S  VP+     + +  T VP RN++FL+LA  YA  +KA+ +  G
Sbjct: 61  ISFFADLAPSALTKSSWSVPEGYYTDESMKQTVVPNRNMVFLSLATSYAISLKAQKLFYG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+A+  ++ L   + +  E P + + K +   +     KL +  
Sbjct: 121 AHAGDHPIYPDCRKEFVEAMKRSILLCDYQIVELEAPYVDLKKEDILKIG---LKLGVDY 177

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           + T +CY G +   CG C  C  R           P   
Sbjct: 178 SLTWSCYKGGE-KACGRCGTCTERIEAFRKIGVKDPIEY 215


>UniRef50_B9MMB0 7-cyano-7-deazaguanine synthase n=8 Tax=Bacteria RepID=QUEC_ANATD
          Length = 221

 Score =  238 bits (608), Expect = 8e-62,   Method: Composition-based stats.
 Identities = 71/219 (32%), Positives = 107/219 (48%), Gaps = 5/219 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVVV SGG DSTT L     Q  EV  ++F YGQ+H  E++ A++    L    +K++D
Sbjct: 2   RAVVVLSGGMDSTTLLYDVKSQGYEVFAISFLYGQKHSKELEFAKKTCSLLSI-PYKIVD 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++   ELA S+LT     VP+       +  T VP RN++ L+++A YA  + A+ +  G
Sbjct: 61  ISFFAELAPSALTTPDWNVPEGYYTDSTMKQTVVPNRNMVLLSISAAYAISLGAKKLFYG 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+A+ +A+ L     +  E P + + K +         +L +  
Sbjct: 121 AHAGDHPIYPDCRKEFVEAMKNALYLADYIGLELEAPYVDMKKEDILRRG---LELGVNY 177

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           + T +CY G +   CG C  C  R           P   
Sbjct: 178 SLTWSCYKGGQ-KACGRCGTCTERIEAFKKVGVKDPIEY 215


>UniRef50_Q1LTK2 7-cyano-7-deazaguanine synthase n=234 Tax=Bacteria RepID=QUEC_BAUCH
          Length = 238

 Score =  236 bits (603), Expect = 3e-61,   Method: Composition-based stats.
 Identities = 154/231 (66%), Positives = 187/231 (80%), Gaps = 1/231 (0%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +K+ VVVFSGGQDSTTCL+QAL QYD+V+C+TFDYGQRHRAEI +AR L + LGA  H++
Sbjct: 7   IKKTVVVFSGGQDSTTCLIQALTQYDQVYCITFDYGQRHRAEICIARRLTITLGAYDHQI 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEA-DGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +D + LN LA SSLTR++I VP  E    +   +TFVPGRNI+FLTLAAIYAYQVKAE +
Sbjct: 67  IDTSALNVLAASSLTRENIIVPLAEKNHMNSSLSTFVPGRNIVFLTLAAIYAYQVKAEVI 126

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           ITGVCETDFSGYPDCR++F++ALN AV+LGMA +I F +PLMW++KAETWALADYY KLD
Sbjct: 127 ITGVCETDFSGYPDCREKFIQALNKAVNLGMAYNIHFISPLMWLNKAETWALADYYQKLD 186

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTGL 230
           LV NETLTCYNG +G GCG C +C+LRA GL  Y       M+++K KTGL
Sbjct: 187 LVCNETLTCYNGIQGKGCGFCTSCSLRARGLETYKQQPKLTMSSLKAKTGL 237


>UniRef50_B2KDX5 ExsB protein n=1 Tax=Elusimicrobium minutum Pei191
           RepID=B2KDX5_ELUMP
          Length = 484

 Score =  236 bits (602), Expect = 4e-61,   Method: Composition-based stats.
 Identities = 85/216 (39%), Positives = 120/216 (55%), Gaps = 8/216 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K+AVV+FSGG DST CL  AL++  E + ++  YGQ+H  EI+VA+ LA + G   HK +
Sbjct: 269 KKAVVLFSGGLDSTVCLYWALKEGYECYALSVAYGQKHLKEIEVAQRLAKQAGVT-HKHI 327

Query: 62  DVTLLNELAVSSLTRD-SIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++ L      +SL     IP    E     IP+T+VP RN++F++L A +A  + AEAV+
Sbjct: 328 EINLPWLAEATSLVGGAEIPATPMEQIGKSIPSTYVPARNLIFVSLGASWADSIGAEAVV 387

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKL 178
            G    D+SGYPDC  +F K L+ AV +G    K     TP++ ++KAE   L      L
Sbjct: 388 LGPNAVDYSGYPDCTPQFYKPLSEAVRVGTRLGKKFEILTPIIKLNKAEIVKLGRK---L 444

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYL 214
                +T TCYNG +   CG C +C LR  G     
Sbjct: 445 GAPLEQTWTCYNGGE-KPCGVCESCKLRRKGFEDAG 479


>UniRef50_A2STX4 7-cyano-7-deazaguanine synthase n=3 Tax=cellular organisms
           RepID=QUEC_METLZ
          Length = 222

 Score =  236 bits (601), Expect = 6e-61,   Method: Composition-based stats.
 Identities = 67/226 (29%), Positives = 104/226 (46%), Gaps = 7/226 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           MK+AV + SGG DSTT    A +   ++  +  +YGQR  R E++ A+++A  LG     
Sbjct: 1   MKKAVCLLSGGMDSTTLAFVAKKMGYDILALHVNYGQRTERKELEAAKKVADVLGVVEFL 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
            + +    +   SSLT  +I V          PNT+VP RN   +++A  Y      EA+
Sbjct: 61  EISLDYFKKFGASSLTDMNIEVEAGVLGRADNPNTYVPFRNGNLISIATSYCESRDGEAI 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLG--MAKDIRFETPLMWIDKAETWALADYYGK 177
             GV   D +GYPDC   F++A+ HA+ +G    K I   TP + ++K +          
Sbjct: 121 FIGVQSGDHTGYPDCTPRFIEAMQHAIYVGTQTEKSIELLTPFVMMNKTDILKEGME--- 177

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           L +    T +CY     + CG C++C  R         + P     
Sbjct: 178 LCVPYEHTWSCY-SENDEACGVCSSCLARLKAFADLGMEDPIPYKK 222


>UniRef50_A0LJR5 7-cyano-7-deazaguanine synthase n=4 Tax=Bacteria RepID=QUEC_SYNFM
          Length = 234

 Score =  234 bits (596), Expect = 2e-60,   Method: Composition-based stats.
 Identities = 85/230 (36%), Positives = 121/230 (52%), Gaps = 13/230 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           ++AVV+FSGG DSTTCL  A  +  E + ++F Y QRH  E++ AR  A  LGAR H VL
Sbjct: 7   RKAVVLFSGGIDSTTCLAVAKAEGFEAYALSFFYHQRHGVEVEAARRSAPALGAREHLVL 66

Query: 62  DVTLLNELAVSSLTRDSIPVPD--YEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++  L+ +  S+LT       D   E  +D IP T+VP RN +FL+ A  +A  + A  +
Sbjct: 67  ELP-LDRIGGSALTGHEEVPKDVPVEKMSDRIPATYVPARNTIFLSFALAWAEVLGAADI 125

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAK------DIRFETPLMWIDKAETWALAD 173
             GV   D+SGYPDCR E++ A     +L + +       I+  TPL+ + K E      
Sbjct: 126 FIGVNALDYSGYPDCRPEYIAAFERMANLAIRETVEGRMRIKVHTPLIRLTKGEIIKRG- 184

Query: 174 YYGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGLNHYLADKPTVMA 222
              +L L  + T +CY+   +G  CGHC +C LR  G        PT   
Sbjct: 185 --LELGLDYSLTHSCYDPDARGLACGHCDSCILRKKGFMEAGIPDPTRYV 232


>UniRef50_B8GJL3 7-cyano-7-deazaguanine synthase n=6 Tax=Euryarchaeota
           RepID=QUEC_METPE
          Length = 221

 Score =  233 bits (595), Expect = 3e-60,   Method: Composition-based stats.
 Identities = 67/224 (29%), Positives = 99/224 (44%), Gaps = 7/224 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +AV + SGG DS+T    A      +H +  +YGQR  + E+  A  +A  L A     L
Sbjct: 2   KAVCLLSGGMDSSTLAYVAKDMGYHIHALHLNYGQRTEKKELQSATAIARSLSAEEFIPL 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+        SSLT  +I V  ++     +PNT+VP RN   L++A  +A    A+A+  
Sbjct: 62  DLGYFKAFGKSSLTDPAIDVDVFDENRPELPNTYVPFRNANLLSIATSFAESRDADAIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRF--ETPLMWIDKAETWALADYYGKLD 179
           GV   D+SGYPDCR  F++A    + LG A        TP + + K +          L 
Sbjct: 122 GVQALDYSGYPDCRPAFIQAFQQVMDLGTADGTHIDLLTPFIGLTKTDILRKGLD---LG 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           +    T +CY   +   CG C +C+ R           P     
Sbjct: 179 VPYEHTWSCYQ-NEEKACGVCGSCHFRQKAFAELGLSDPIPYEE 221


>UniRef50_A3ZMY4 Predicted PP-loop superfamily ATPase n=1 Tax=Blastopirellula marina
           DSM 3645 RepID=A3ZMY4_9PLAN
          Length = 219

 Score =  233 bits (593), Expect = 6e-60,   Method: Composition-based stats.
 Identities = 68/223 (30%), Positives = 101/223 (45%), Gaps = 4/223 (1%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M + V V SGG DS T L   +    +V  ++ +YGQRH  E+D AR+L  K+G      
Sbjct: 1   MSKVVAVVSGGMDSATLLYHLIDAGHDVRAISINYGQRHVKELDYARQLCDKVGVPQIVA 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
               +      +SL+   I +P+     + +  T VP RN++ L+++  +A   +  AV 
Sbjct: 61  DLSAINPLFGANSLSGREIEIPEGHYAEENMKLTVVPNRNMILLSVSIAWAISQECVAVA 120

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G    D + YPDCR EF  A++ A  L     +    P + IDK +    A    +L +
Sbjct: 121 YGAHSGDHAIYPDCRPEFAAAMDQAAKLCDWSAVELWRPFVDIDKGDI---AQRGSELGV 177

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
               T TCY G   + CG C AC  R      +    PT   A
Sbjct: 178 PYELTWTCYKGL-AEHCGKCGACVERKEAFAKHGLVDPTAYMA 219


>UniRef50_Q8YM45 7-cyano-7-deazaguanine synthase n=14 Tax=cellular organisms
           RepID=QUEC_ANASP
          Length = 229

 Score =  231 bits (589), Expect = 1e-59,   Method: Composition-based stats.
 Identities = 80/231 (34%), Positives = 116/231 (50%), Gaps = 13/231 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV++ SGG DS+T L QA     E + ++FDY QRHR E+  A  +A   G   H+V++
Sbjct: 2   KAVILLSGGLDSSTILYQAKADGCECYSISFDYQQRHRRELHSAFLVAQTAGIVQHQVIN 61

Query: 63  VTLLNELAVSSLTRDSIPVPDYE---PEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
              L     S+LT D+I +P        +  IP T+VP RN +FL+ A  YA  + A+ V
Sbjct: 62  FD-LRLWGGSALTDDNIDLPQERSLDAMSQNIPVTYVPARNTIFLSFALAYAEAIAAQRV 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADY 174
             GV   D+SGYPDCR ++++A+     LG       + I    PL+ + K E   L + 
Sbjct: 121 YIGVNALDYSGYPDCRPDYIEAMQEVFRLGTKQGREGQPINIVAPLINLKKTEIIQLGNQ 180

Query: 175 YGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
              L +  N T +CY G     CG C +C LR         + P   A +K
Sbjct: 181 ---LGVPWNLTWSCYAGG-DVACGVCDSCRLRLAAFAELGLEDPLPYAYLK 227


>UniRef50_A8EWF5 7-cyano-7-deazaguanine synthase n=27 Tax=Epsilonproteobacteria
           RepID=QUEC_ARCB4
          Length = 228

 Score =  230 bits (586), Expect = 3e-59,   Method: Composition-based stats.
 Identities = 74/225 (32%), Positives = 115/225 (51%), Gaps = 8/225 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKV 60
           K+A+ + SGG DST     A +   E+  V F+YGQR +  E+   R++  +L  ++   
Sbjct: 7   KKAICILSGGMDSTLSSYIAKKDGFEIIAVHFNYGQRTQNRELKAFRDICNELDIKSKYE 66

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+    ++  ++LT  SI VP    EA G+P T+VP RN +FL + A  A +  A A+ 
Sbjct: 67  IDIPFFTQIGANALTDMSIDVPTGGLEA-GVPITYVPFRNGIFLAITAAIAEKEGATAMY 125

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKL 178
            GV + D SGYPDC +EF++ +  A++ G      I   TPL+ + KA+    A     L
Sbjct: 126 IGVVQEDSSGYPDCTEEFIQDMKKAINQGTKEDTKIEIITPLVHLSKAQIVQEAIK---L 182

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           ++    T +CY   + + CG C +C LR NG        P     
Sbjct: 183 NVPLELTWSCYK-EEEEACGVCDSCRLRLNGFEQAGKKDPIKYKE 226


>UniRef50_A8GPK3 7-cyano-7-deazaguanine synthase n=18 Tax=Proteobacteria
           RepID=QUEC_RICAH
          Length = 227

 Score =  229 bits (585), Expect = 5e-59,   Method: Composition-based stats.
 Identities = 76/231 (32%), Positives = 119/231 (51%), Gaps = 13/231 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AV++ SGG DS T L  A +   E+H ++F+YGQR+ AE+   +EL  +   + HK+
Sbjct: 1   MKKAVMLVSGGADSATVLAMAREIGYEIHSMSFNYGQRNNAELRKVKELIKEYNVKQHKI 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEA---DGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +D+  L     S+LT D+I VP Y       + +P T+VP RN +FL+ A  YA  + A+
Sbjct: 61  VDID-LRAFGGSALTDDNIDVPHYHDVNAVPEDVPVTYVPSRNTIFLSYALGYAEVIGAK 119

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALA 172
            +  GV  +D + YPDCR E++K+     +L        + I   TPL+ + K +     
Sbjct: 120 DIFIGVHTSDSANYPDCRPEYIKSFEKMANLATSIGIQGQKITIHTPLIDMTKEQIIRTG 179

Query: 173 DYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVMA 222
               KL +    T++CY   + D  CG+C AC +R +         P    
Sbjct: 180 ---LKLGVDYKNTISCYEPTEDDSSCGNCLACMIRLDAFKKNNIQDPIKYV 227


>UniRef50_B2UN88 ExsB protein n=6 Tax=Bacteria RepID=B2UN88_AKKM8
          Length = 223

 Score =  229 bits (583), Expect = 6e-59,   Method: Composition-based stats.
 Identities = 66/226 (29%), Positives = 105/226 (46%), Gaps = 7/226 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           M+ AV + SGG DS+  L  A + +     ++FDY   H   E+  A   A  LG   H+
Sbjct: 4   METAV-LLSGGLDSSVALHWAHRNHRVRVALSFDYASNHAERELKCAAWQAASLGIE-HR 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V+D++ ++    S+L   +  +PD   + + +  T VP RN +FL++AA  A    A+ +
Sbjct: 62  VIDISSISSHLKSALLSGADDIPDGSYDEELMKQTVVPFRNGIFLSIAAGVAESCGAQQL 121

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +      D S YPDCR+EF+ A+  A+ LG    +    P + + K    A+      L 
Sbjct: 122 VIAAHSGDHSIYPDCREEFMAAMTEAIRLGTYAGLGILRPFIHMSKGGIAAMGQD---LG 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
           +  + T +CY G     CG C+ C  R           PT  A  +
Sbjct: 179 VDFSRTYSCYKGG-AVHCGVCSTCMERREAFREAGIPDPTAYAPAE 223


>UniRef50_B2I4Y4 7-cyano-7-deazaguanine synthase n=16 Tax=Bacteria RepID=QUEC_XYLF2
          Length = 230

 Score =  229 bits (583), Expect = 7e-59,   Method: Composition-based stats.
 Identities = 82/230 (35%), Positives = 112/230 (48%), Gaps = 13/230 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AV++ SGG DS      A  Q   VH ++  YGQRH +E+D A  +A  L   AHKV
Sbjct: 1   MKKAVILLSGGMDSAVVTAIAQSQGFMVHALSIRYGQRHTSELDAAVRIARALNVVAHKV 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +DV  L  +  S+LT D I +PD     +GIP T+VP RN + L+LA  +A  + A  + 
Sbjct: 61  VDVD-LRSIGGSALTDD-IEIPDA--GGEGIPVTYVPARNTIMLSLALGWAEVIGAADIF 116

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADYY 175
            GV   D+SGYPDCR +F+ A     +L          +    PL ++ KAE        
Sbjct: 117 CGVNAVDYSGYPDCRPQFITAFETLANLATKVGVEGTQLHVHAPLQFLSKAEIV---HEG 173

Query: 176 GKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
               +    T++CY     G  CG C AC LR  G        PT    +
Sbjct: 174 LLHGVDFGLTVSCYRADVDGRACGRCDACKLRVAGFADAGVVDPTRYMEL 223


>UniRef50_B8HQ99 7-cyano-7-deazaguanine synthase n=1 Tax=Cyanothece sp. PCC 7425
           RepID=QUEC_CYAP4
          Length = 229

 Score =  227 bits (579), Expect = 2e-58,   Method: Composition-based stats.
 Identities = 72/232 (31%), Positives = 110/232 (47%), Gaps = 16/232 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V++ SGG DSTT L  A     + + ++F+YGQRH+ E+  A  +A   G   H+V+ 
Sbjct: 2   KIVILLSGGLDSTTVLYLARSLGHDCYALSFNYGQRHKQELAAAIAVAQLAGVIQHQVIS 61

Query: 63  VTLLNELAVSSLTRDSIPVPDYE---PEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
              L     S+LT  ++ +P        A+ IP T+VP RN +FL+ A  YA  + A  V
Sbjct: 62  FD-LGLWGGSALTDYTLDLPQNRAFKEMAEQIPITYVPARNTIFLSFALSYAEAIGATQV 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADY 174
             G+   D+SGYPDCR +++ A+     LG       + I+  TPL+ + K     L   
Sbjct: 121 YAGMNAIDYSGYPDCRPDYIAAMQEVYRLGTKQGREGEPIQIITPLIDLTKPAIIQLG-- 178

Query: 175 YGKLDLVRNETLTCYNGFKG----DGCGHCAACNLRANGLNHYLADKPTVMA 222
             +L +   +T +CY+   G      CG C +C LR           P    
Sbjct: 179 -NRLGVPWAKTWSCYSDGGGNDPPVACGVCDSCRLRLAAFAELSLTDPLPYR 229


>UniRef50_O29807 7-cyano-7-deazaguanine synthase n=1 Tax=Archaeoglobus fulgidus
           RepID=QUEC_ARCFU
          Length = 239

 Score =  227 bits (578), Expect = 3e-58,   Method: Composition-based stats.
 Identities = 74/217 (34%), Positives = 107/217 (49%), Gaps = 6/217 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV++ SGG DS+T L   L    EVH +TF YGQ+H  EI+ A ++A     R  KV  
Sbjct: 2   KAVMLLSGGIDSSTLLYYLLDGGYEVHALTFFYGQKHSKEIESAEKVAKAAKVRHLKVDI 61

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
            T+ + ++  +LT +   VP      +    T VP RN++ L++AA YA ++ A+ V   
Sbjct: 62  STIHDLISYGALTGEE-EVPKAFYSEEVQRRTIVPNRNMILLSIAAGYAVKIGAKEVHYA 120

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLG-MAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              +D+S YPDCR EFVKAL+ AV L  +   +    P + + KA+   L     KL + 
Sbjct: 121 AHLSDYSIYPDCRKEFVKALDTAVYLANIWTPVEVRAPFVDMTKADIVRLG---LKLGVP 177

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
              T +CY G     C  C  C  R           P
Sbjct: 178 YELTWSCYEGG-DRPCLSCGTCLERTEAFLANGVKDP 213


>UniRef50_Q1QVW9 7-cyano-7-deazaguanine synthase n=23 Tax=Proteobacteria
           RepID=QUEC_CHRSD
          Length = 251

 Score =  226 bits (576), Expect = 5e-58,   Method: Composition-based stats.
 Identities = 75/222 (33%), Positives = 109/222 (49%), Gaps = 4/222 (1%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AVV++SGG DS T L +AL+   EVH ++F YGQRH  E++ A ++  +LG     V  
Sbjct: 30  KAVVIYSGGMDSYTVLHRALRAGFEVHALSFHYGQRHSRELETAHDVCQRLGIAHQVVDI 89

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
             +   +  S+LT  +  +PD + +AD +  T VP RN++ L+LA  +A  + A     G
Sbjct: 90  RAIHGLIGNSALTDATQTMPDGDYDADNMAATVVPNRNMILLSLAIGHAVNIGANVCFYG 149

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
               D   YPDCR EFV+ +N   ++     +R   P +   K E  A     G   L  
Sbjct: 150 AHGGDHVLYPDCRPEFVERMNDVAAIADFTPVRIAAPYLHASKEEILADGLAMG---LDY 206

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
            +T TCY G +   CGHC +C  R           P V A  
Sbjct: 207 AQTWTCYLGAE-RSCGHCGSCRERLAAFAAQGVTDPLVYAGA 247


>UniRef50_Q55468 7-cyano-7-deazaguanine synthase n=43 Tax=Bacteria RepID=QUEC_SYNY3
          Length = 232

 Score =  225 bits (573), Expect = 1e-57,   Method: Composition-based stats.
 Identities = 74/220 (33%), Positives = 109/220 (49%), Gaps = 10/220 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K AVV+ SGG DS T    A ++   V  ++F+YGQRH  E+  A ++   LG   H  +
Sbjct: 3   KTAVVLLSGGLDSATVAAIAKREGYRVIALSFNYGQRHDRELRAAADIVQALGIPEHFSI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++  L +   SSLT     +P    E D IP+T+VPGRN +F+ L    A    AEAV  
Sbjct: 63  NLD-LAQWGGSSLTDRQQTLPQTGVEPDIIPSTYVPGRNTVFIALGLSLAEAKGAEAVFL 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALADYYG 176
           G+   D+SGYPDCR E++       +L        + I+   PL+ + K +    A    
Sbjct: 122 GINAIDYSGYPDCRPEYLATYQQLAALSSKVGVEGRPIQLLAPLIELSKVDIVHKA---L 178

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
           +L +   +T +CY G + + CG C +C LR   L      
Sbjct: 179 ELGVPIAQTWSCYAGGE-EPCGRCDSCRLRDQALIEAGHP 217


>UniRef50_C8R2H0 ExsB protein n=1 Tax=Desulfurivibrio alkaliphilus AHT2
           RepID=C8R2H0_9DELT
          Length = 258

 Score =  225 bits (573), Expect = 1e-57,   Method: Composition-based stats.
 Identities = 87/245 (35%), Positives = 122/245 (49%), Gaps = 25/245 (10%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVV+ SGG DSTT L  A       HC++F YGQRH  E+++AR  A   GA  H +L++
Sbjct: 18  AVVLLSGGLDSTTVLALAKAAGYRCHCLSFRYGQRHTQELELARANARHWGAARHLILNI 77

Query: 64  TLLNELAVSSLTRDSIPVP---------------DYEPEADGIPNTFVPGRNILFLTLAA 108
             L+ +  S+LT D + VP               D    A  +P T+VPGRN +FL  AA
Sbjct: 78  -ELDAIGGSALTGDELEVPKDNLSPLDSPDGAETDAPGMAGEVPVTYVPGRNTIFLAYAA 136

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWI 163
            +A  + A  +  GV   DFSGYPDCR ++++AL  A++LG       +  R + PL+ +
Sbjct: 137 SWAEVLGARDIFIGVNAVDFSGYPDCRPDYLEALAAALNLGSQCGREGRPFRLQAPLLHL 196

Query: 164 DKAETWALADYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVMA 222
            KAE  A       L +    T +CY+  +    CG C AC LR  G        PT   
Sbjct: 197 SKAEIIAKG---LALGVDYARTHSCYDPDQQHRACGRCDACILRRRGFAAAGCPDPTTYQ 253

Query: 223 AMKQK 227
             + +
Sbjct: 254 PARSE 258


>UniRef50_B3QPB3 7-cyano-7-deazaguanine synthase n=4 Tax=cellular organisms
           RepID=QUEC_CHLP8
          Length = 227

 Score =  224 bits (572), Expect = 1e-57,   Method: Composition-based stats.
 Identities = 67/222 (30%), Positives = 98/222 (44%), Gaps = 7/222 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +AV++ SGG DS      A     E+  +  +YGQR  + E++  R++    G      +
Sbjct: 2   KAVLLVSGGMDSLVATALAHHHGFELAAMHVNYGQRTWQKELECFRQICDHYGIEKRLEI 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D   L  +  SSLT  +IPV   +     IP ++VP RN  FL++A  ++  + A  +  
Sbjct: 62  DAGFLGAIGGSSLTDAAIPVGPADLAGTDIPTSYVPFRNANFLSMAVSWSEVIGANRIYI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKLD 179
           G  E D SGYPDCR  F  A N  +  G      I   TPL+ + KAE          LD
Sbjct: 122 GAVEEDSSGYPDCRKVFYDAFNQVIEHGTRPETSIEIVTPLIDMSKAEIVKRGME---LD 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              + + +CY   +G  CG C +C  R           P   
Sbjct: 179 APFHFSWSCYKS-EGKACGVCDSCARRLRAFASVGIADPVEY 219


>UniRef50_B0S9S9 7-cyano-7-deazaguanine synthase n=13 Tax=Bacteria RepID=QUEC_LEPBA
          Length = 248

 Score =  224 bits (571), Expect = 2e-57,   Method: Composition-based stats.
 Identities = 80/237 (33%), Positives = 114/237 (48%), Gaps = 24/237 (10%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD-------EVHCVTFDYGQRHRAEIDVARELALKLG 54
           K A+V+ SGG DSTTCL  A + +         +  ++FDY Q+H+ E+  ++++A  LG
Sbjct: 16  KGAIVLLSGGLDSTTCLYLAAKDFGYPKNKKLPLLALSFDYSQKHKIELTKSKKIAKTLG 75

Query: 55  ARAHKVLDVTLLNELAVSSLTRDSIPVPDYEP-----EADGIPNTFVPGRNILFLTLAAI 109
            + H +  +        SSLT   I V          +   IPNT+VPGRNILFL+ A  
Sbjct: 76  IK-HVIQKLDP-GFFLGSSLTESKIKVRKNAKSLFSGDETEIPNTYVPGRNILFLSFALS 133

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD-------IRFETPLMW 162
            A     +++  GV   D+SGYPDCR EF+ +     +LG  K        I+ +TPL+ 
Sbjct: 134 LAEGHGYDSIYIGVNALDYSGYPDCRPEFIDSFQKMANLGTKKGVSGTGDSIQIKTPLLH 193

Query: 163 IDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
           + K E   L    G        T +CY+   G  CG C +C LRA G        P 
Sbjct: 194 LGKKEIIELGMSVG---APLGLTHSCYDPVSGKPCGKCDSCILRAKGFLEVGLKDPA 247


>UniRef50_A7HHA2 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria RepID=QUEC_ANADF
          Length = 233

 Score =  224 bits (570), Expect = 3e-57,   Method: Composition-based stats.
 Identities = 90/229 (39%), Positives = 120/229 (52%), Gaps = 12/229 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           KRAVV+ SGG DSTTCL  A +   E HC++ DYGQRH+ E+  AR L+  LGA  H+V+
Sbjct: 7   KRAVVLLSGGLDSTTCLAVARRDGFEAHCLSVDYGQRHKGELARARRLSRALGAADHRVV 66

Query: 62  DVTLLNELAVSSLTRDSIPVPD---YEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            V  L+    S+LT  +I VP        A  IP T+VP RN + L LA  +A  + AE 
Sbjct: 67  KVD-LSAFGGSALTDRAIAVPKGRSGRRRAAEIPVTYVPARNTVLLALALAHAETLGAED 125

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETPLMWIDKAETWALAD 173
           V  GV   D+SGYPDCR  F++A     ++        + +R   PL+ + KA    L  
Sbjct: 126 VYVGVNAIDYSGYPDCRPAFLRAFERLATVATKAGVEGRPLRIHAPLLRLTKAGIVRLGT 185

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
               L +    TL+CY+  +G  CG C AC LR  G        PT+  
Sbjct: 186 S---LGVPYRVTLSCYDPVRGRACGACDACVLRRKGFAEAGIPDPTLYV 231


>UniRef50_C1D6L0 7-cyano-7-deazaguanine synthase n=6 Tax=Bacteria RepID=QUEC_LARHH
          Length = 229

 Score =  224 bits (570), Expect = 3e-57,   Method: Composition-based stats.
 Identities = 92/217 (42%), Positives = 128/217 (58%), Gaps = 3/217 (1%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + A+VV SGGQDSTTCL  AL ++ +V  ++FDYGQRHR E+D AR +A   G   H ++
Sbjct: 8   EHALVVLSGGQDSTTCLYWALSRFAQVSAISFDYGQRHRVELDAARTIAAMAGV-GHTII 66

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            +   + L  ++LT   +           +PNTFVPGRN++FLT AA +A+      ++T
Sbjct: 67  PINTFSALGGNALTDQQMSPDTGPDAETLLPNTFVPGRNLVFLTFAAAWAWPRGIRHIVT 126

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GV +TD+SGYPDCR+  ++AL  A++LGM   +R   PLM++ KA+T  LA   G +   
Sbjct: 127 GVAQTDYSGYPDCRENTLRALELAINLGMESRMRLHMPLMFLSKADTVTLARTVGAMPA- 185

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
              + TCY G     CG CAAC LRA G        P
Sbjct: 186 LAFSHTCYAGA-VPPCGQCAACVLRAKGFAEAGIPDP 221


>UniRef50_B9Z9V1 ExsB protein n=2 Tax=Halobacteriaceae RepID=B9Z9V1_NATMA
          Length = 251

 Score =  223 bits (568), Expect = 4e-57,   Method: Composition-based stats.
 Identities = 75/224 (33%), Positives = 107/224 (47%), Gaps = 7/224 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           RAVV+ SGG DS T   +A  Q  +++ +   YGQR    E++ AR+LA +  A     +
Sbjct: 24  RAVVLLSGGMDSATAAAEARSQGYDIYALHTSYGQRTETRELECARQLADEFEANDFLQI 83

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +   L+ +  SSLT D + V D + ++D IP+++VP RN   L +A  YA     EAV  
Sbjct: 84  ETGHLSAIGASSLTDDEMAVADADMDSDEIPSSYVPFRNANLLAMAVSYAEANDCEAVFI 143

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKLD 179
           G    DFSGYPDCR EF +A      +G     +I  E P +   K +    A +  +L 
Sbjct: 144 GAHSEDFSGYPDCRPEFFEAFERVADVGTKPETEISIEAPFVEWSKTDI---AAHGTELG 200

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           +    T +CY   +   CG C AC  R           P   A 
Sbjct: 201 VPYEHTWSCYRDDQP-ACGTCDACAYRLQAFQRIGVRDPIEYAE 243


>UniRef50_Q8K986 7-cyano-7-deazaguanine synthase n=1 Tax=Buchnera aphidicola
           (Schizaphis graminum) RepID=QUEC_BUCAP
          Length = 232

 Score =  222 bits (566), Expect = 7e-57,   Method: Composition-based stats.
 Identities = 104/227 (45%), Positives = 152/227 (66%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+ +++FSGGQDSTTCL+     Y E++C+TFDY Q H++EID AR ++     + H  
Sbjct: 1   MKKILIIFSGGQDSTTCLIHYTNIYKEIYCITFDYNQLHKSEIDSARFISNYFNVKKHIF 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           +D+  L  L++SSLT + I + D  P    +P+TFVPGRNILFLTL++IYA+  +  +++
Sbjct: 61  VDLKCLKNLSISSLTDEKISILDNHPLNFSLPSTFVPGRNILFLTLSSIYAFNHQINSIV 120

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            GV E DFSGYPDCR+ F+K +N  V +GM   I F++PL+ + KAE WAL+DY+     
Sbjct: 121 LGVNEIDFSGYPDCRNAFLKKMNDVVQIGMNCKINFQSPLINLSKAEIWALSDYWNSTQF 180

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQK 227
           + N T+TCY G +G GCG C +C LR +G N + ++    M  +K+K
Sbjct: 181 ILNNTVTCYQGIQGKGCGQCQSCILRNDGFNKWKSNPSYYMKKLKEK 227


>UniRef50_Q1QCP3 7-cyano-7-deazaguanine synthase n=122 Tax=Bacteria RepID=QUEC_PSYCK
          Length = 259

 Score =  222 bits (566), Expect = 7e-57,   Method: Composition-based stats.
 Identities = 86/237 (36%), Positives = 121/237 (51%), Gaps = 22/237 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVV+ SGG DS TCL  A  +Y  V  V+FDYGQRH +E+  A+ +A   G   H+++D+
Sbjct: 28  AVVLLSGGLDSVTCLYWAKARYASVTAVSFDYGQRHNSELVAAKAIAETAGV-NHRIIDI 86

Query: 64  TLLNELAVSSLTRDSIPVPDY-----------EPEADGIPNTFVPGRNILFLTLAAIYAY 112
            +  +L  SSLT  S+ VPD            E + D IPNT+VP RN +FL+ A   A 
Sbjct: 87  DI-AQLGGSSLTDHSMIVPDGDTDKFPDKKRDEIDNDAIPNTYVPARNTIFLSYALAVAE 145

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR-----FETPLMWIDKAE 167
              +  ++ GV   D+SGYPDCR E++ A  H  +L     +       +TPL  + KA+
Sbjct: 146 VTDSNHIVIGVSSVDYSGYPDCRPEYIAAFQHMANLATKAGVTGHHLSIQTPLQQLSKAK 205

Query: 168 TWALADYYGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           T  L      L +   +T++CY     G  CG C +C LR  G        PT   +
Sbjct: 206 TIELG---LSLGVDYGQTISCYQADANGLACGVCDSCALRRQGFAQAGVADPTHYQS 259


>UniRef50_C1YMB1 PreQ(0) biosynthesis protein QueC n=1 Tax=Nocardiopsis dassonvillei
           subsp. dassonvillei DSM 43111 RepID=C1YMB1_NOCDA
          Length = 243

 Score =  221 bits (563), Expect = 2e-56,   Method: Composition-based stats.
 Identities = 76/221 (34%), Positives = 95/221 (42%), Gaps = 6/221 (2%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
             VVVFSGG DSTT L         +  VT +YGQRH  E+D AR +A   GA  H +  
Sbjct: 17  TVVVVFSGGMDSTTLLAHFAALRFRLVAVTVEYGQRHGKEVDAARAIARHYGAEHHILGI 76

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                 L  S+LT   + VPD     + + +T VP RN +   LAA  A   +A  V  G
Sbjct: 77  AGFGPLLRGSALTDARVEVPDGHYAEESMRDTVVPNRNAVLANLAASVAVARRAGTVALG 136

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAK--DIRFETPLMWIDKAETWALADYYGKLDL 180
           +   D   YPDCR  FV AL H V +        R E P M   KA+    A +  +L  
Sbjct: 137 MHSGDHFVYPDCRPAFVDALRHLVGVANEGFAAPRVEAPFMAWSKADI---ARHGVRLGA 193

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
               + +CY G     CG C  C  R           PT  
Sbjct: 194 PLEHSWSCYKGG-DIHCGTCGTCYERREAFQEAGLPDPTEY 233


>UniRef50_B2RJS2 7-cyano-7-deazaguanine synthase n=5 Tax=Bacteria RepID=QUEC_PORG3
          Length = 220

 Score =  220 bits (560), Expect = 3e-56,   Method: Composition-based stats.
 Identities = 60/223 (26%), Positives = 96/223 (43%), Gaps = 8/223 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           K +++V SGG DS T + +  ++      ++FDYG +H   E+  AR  A +LG   H +
Sbjct: 5   KDSLIVVSGGMDSVTLMYE--KRASIALALSFDYGSKHNARELSFARLHAERLGVE-HLI 61

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  + +   S L      +P+   + + + +T VP RN + L +AA  A       + 
Sbjct: 62  IPLDFIGQYFQSDLLLSGGDIPEGRYDEENMKSTVVPFRNGIMLAVAAGLAESRGLRRIY 121

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D + YPDCR  F++ +  AV  G    +  E P   I K +   +      L +
Sbjct: 122 IANHFGDHAIYPDCRASFIRPMTEAVRCGTTNGVLIEAPYTDITKTDIARIGAS---LGI 178

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
              ET +CY G     CG C  C  R   L+      PT    
Sbjct: 179 DYAETWSCYKGG-VFHCGVCGTCVERKEALHDAGIPDPTEYEG 220


>UniRef50_C0QST3 7-cyano-7-deazaguanine synthase n=7 Tax=Aquificales
           RepID=QUEC_PERMH
          Length = 227

 Score =  218 bits (556), Expect = 1e-55,   Method: Composition-based stats.
 Identities = 74/221 (33%), Positives = 112/221 (50%), Gaps = 12/221 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
            +++ SGG DS   L  A +++D+V+ V+FDYGQ+HR E+D A++L  + G   H + +V
Sbjct: 7   IIILVSGGMDSAALLWLAKKEFDKVYAVSFDYGQKHRVELDFAKKLTEEAGVEEHFIAEV 66

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             L  +  S+LT  S+ +P  E   +G P T VP RN+ FL++AA +A   + E +  GV
Sbjct: 67  PHLKGIKGSALTDRSVEIPSEEY-PEGPPVTTVPMRNLNFLSIAASFADIYEIENIGIGV 125

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGM------AKDIRFETPLMWIDKAETWALADYYGK 177
              D S YPDCR EFV A   A++            I   TP + + K +   +     +
Sbjct: 126 HSVD-SPYPDCRAEFVSAAEAAINASSVMVAKKKNRITVYTPFLGMTKTDVLRIG---LE 181

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
           L +   +T +CY G     CG CA C  R         + P
Sbjct: 182 LGVPYEKTYSCYRGTIP-PCGECATCRQRIEAFRSVGLEDP 221


>UniRef50_Q2N612 7-cyano-7-deazaguanine synthase n=56 Tax=Bacteria RepID=QUEC_ERYLH
          Length = 239

 Score =  218 bits (555), Expect = 1e-55,   Method: Composition-based stats.
 Identities = 79/227 (34%), Positives = 108/227 (47%), Gaps = 13/227 (5%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K A ++ SGG DS      A +Q   VH +T DYGQRH+ E+  AR +A K+GA  H V 
Sbjct: 7   KAAAILLSGGLDSMVTAAIAQEQGYAVHALTIDYGQRHKVELQSARLIAAKIGAERH-VE 65

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
               L  L  S+LT D I VP      D IP T+VP RN++FL+L   +A    A  +  
Sbjct: 66  IEADLRALGGSALTDD-IDVPKCGVGND-IPVTYVPARNLVFLSLTTAFAEASGARDIFI 123

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI------RFETPLMWIDKAETWALADYY 175
           GV   D+SGYPDCR EF+++     +L     +      +   PL  + KA+    A   
Sbjct: 124 GVNALDYSGYPDCRPEFIESFAVTANLATKAGVEESAPFKIHAPLQHMTKADI---AREC 180

Query: 176 GKLDLVRNETLTCYNGFKG-DGCGHCAACNLRANGLNHYLADKPTVM 221
            +L L    + +CY+   G   CG C +C LR  G            
Sbjct: 181 ARLGLESAWSWSCYDPLPGLKPCGACDSCRLRRKGFAEAGLADGLDY 227


>UniRef50_B6CWJ6 ToyM n=3 Tax=Bacteria RepID=B6CWJ6_STRRM
          Length = 238

 Score =  217 bits (553), Expect = 2e-55,   Method: Composition-based stats.
 Identities = 75/226 (33%), Positives = 99/226 (43%), Gaps = 6/226 (2%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AVVV SGG DSTT L         +  VT DYGQRHR EID AR +A   GA  H V   
Sbjct: 13  AVVVLSGGMDSTTLLAHYAMLRYRLTAVTVDYGQRHRREIDAARTIAGHYGAEHHVVDLS 72

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            +   L  S+LT   + VP      + +  T VP RN +   +A   A   +A  V  G+
Sbjct: 73  GIGVLLGGSALTDSEVDVPSGHYAEESMRATVVPNRNAILANVAVGAAVARQAGIVALGM 132

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAK--DIRFETPLMWIDKAETWALADYYGKLDLV 181
              D   YPDCR  FV+AL+  V +        R E P +   K +    A Y  +L+  
Sbjct: 133 HAGDHFVYPDCRPAFVEALDGLVRVANEGFATPRVEAPFIHWSKTDI---ARYGSRLEAP 189

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQK 227
            +++ +CY G +   CG C  C  R           PT       +
Sbjct: 190 LDKSWSCYRGGE-LHCGTCGTCYERREAFRDADVPDPTEYLDAATE 234


>UniRef50_A0B6A6 7-cyano-7-deazaguanine synthase n=4 Tax=Archaea RepID=QUEC_METTP
          Length = 224

 Score =  216 bits (549), Expect = 7e-55,   Method: Composition-based stats.
 Identities = 63/230 (27%), Positives = 98/230 (42%), Gaps = 13/230 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           M RAV + SGG DST     A +   +V+ +   YGQ+  R EI+    +A  +GA    
Sbjct: 1   MGRAVCLCSGGLDSTVAATIARRSGMDVYLIHVSYGQQAERREIEAIERIADAIGASDLM 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEP---EADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
              + L   +  S+LT     +P  E    +++  P T+V  RN + L++AA YA  + A
Sbjct: 61  CSRIDLFRNI--SALTTQGARIPRGEEVSLDSESTPPTWVYCRNTVLLSMAAAYAEYLGA 118

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMA---KDIRFETPLMWIDKAETWALAD 173
            ++  G    +   YPD R EFV+  N  +   +A   +  +   PL+ + K +   L  
Sbjct: 119 HSIYVGFNAEEAMSYPDNRPEFVEQFNALLEKAVASFSRPPKVVAPLVDMRKKDIVRLGA 178

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
                      T +CY   +   CG C +C  R  G        PT    
Sbjct: 179 DI---KAPLELTWSCYLNGEI-HCGTCESCQHRRRGFVEAGIPDPTEYQH 224


>UniRef50_Q3AR02 7-cyano-7-deazaguanine synthase n=12 Tax=Bacteria RepID=QUEC_CHLCH
          Length = 228

 Score =  216 bits (549), Expect = 7e-55,   Method: Composition-based stats.
 Identities = 68/222 (30%), Positives = 102/222 (45%), Gaps = 7/222 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS      A Q   E+  +  +YGQR  + E++  R +      +    +
Sbjct: 2   KAILLLSGGMDSLVTTAIAQQAGFELAAMHVNYGQRTMQRELNSFRAICSHYSIQQRLEI 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +   L ++  SSLT  S+PV     E+  IP ++VP RN  FL++A  +A  + AE +  
Sbjct: 62  NADFLGKIGGSSLTDLSMPVSVANLESHAIPASYVPFRNAGFLSMAVSWAEVIGAERIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM--AKDIRFETPLMWIDKAETWALADYYGKLD 179
           G  E D SGYPDCR  F +A N  + LG      I   TPL+ + K E          L 
Sbjct: 122 GAVEEDSSGYPDCRKIFYEAFNRVIELGTKPETHIEVVTPLIALKKWEIVRKGIE---LH 178

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                + +CY   +G  CG C +C LR         + P   
Sbjct: 179 APFAFSWSCYK-NEGQACGVCDSCALRLRAFEQAGMEDPIDY 219


>UniRef50_D1PYH8 Transcription regulator ExsB n=5 Tax=Prevotella RepID=D1PYH8_9BACT
          Length = 242

 Score =  215 bits (548), Expect = 8e-55,   Method: Composition-based stats.
 Identities = 63/224 (28%), Positives = 97/224 (43%), Gaps = 8/224 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           + A+++ SGG DS T L +   +      ++FDYG  H   EI  A     +LG   H +
Sbjct: 26  RDAIIIVSGGMDSITMLYEFQDRI--ALGLSFDYGSNHNAREIPYAEMHCKRLGI-PHII 82

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           + +  ++    SSL   +  +P+ +    G+ +T VP RN + L++AA  A     + ++
Sbjct: 83  IPLDFMSRYFKSSLLEGADAIPEGDYNEVGMKSTVVPFRNGIMLSIAAGMAESQNLKYIM 142

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 D   YPDCR EF++A++ A   G    I+   P   I K +    A     L L
Sbjct: 143 MANHSGDHIIYPDCRPEFIRAMSKATQTGTFPGIKILAPYTDITKGDI---ACRGKALGL 199

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
              ET +CY G +   CG C  C  R   L        T     
Sbjct: 200 DYTETWSCYKGGE-RHCGKCGTCLERIAALKDAGIKDLTEYEEE 242


>UniRef50_Q6ARX9 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria RepID=QUEC_DESPS
          Length = 219

 Score =  214 bits (545), Expect = 2e-54,   Method: Composition-based stats.
 Identities = 74/222 (33%), Positives = 106/222 (47%), Gaps = 6/222 (2%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           M  +++V SGG DS   L Q L++      ++FDYG +H   E+ +A  L  +   + H+
Sbjct: 1   MVDSIIVLSGGMDSAVLLAQTLKEGKTAQAISFDYGSKHNDRELPMAVGLCEEFAIK-HQ 59

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++ +  + EL  SSL      +PD     + + +T VP RN + L +AA YA  V+A  V
Sbjct: 60  IIKLPFIGELFSSSLLTGGEEIPDGAYGQENMKSTVVPFRNGIMLAIAAGYAESVQAGEV 119

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           + G    D   YPDCR EF  A   AV+LG    +    P   + K +   L      LD
Sbjct: 120 LLGSHSGDHHIYPDCRPEFNNAFAEAVNLGTDGQVAIRFPFSEMTKRDIGDLGR---TLD 176

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           L   +T TCY G +   CG CAAC+ R   L H      T  
Sbjct: 177 LDFAKTWTCYKGGE-LHCGVCAACDERKEALRHPEGLDVTKY 217


>UniRef50_Q12WI3 7-cyano-7-deazaguanine synthase n=6 Tax=cellular organisms
           RepID=QUEC_METBU
          Length = 233

 Score =  213 bits (543), Expect = 3e-54,   Method: Composition-based stats.
 Identities = 63/236 (26%), Positives = 104/236 (44%), Gaps = 16/236 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           R++++ S G DS   L  AL   D    +TFDYGQR  + EI+ +R +    G   H+++
Sbjct: 2   RSILLLSSGLDSVAALAIALDNSDVFMALTFDYGQRSVKKEIEYSRMVCEHYGIE-HRII 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPE----------ADGIPNTFVPGRNILFLTLAAIYA 111
            ++ L+ +  +SL    + VP+               D   + +VP RN +F+ +AA +A
Sbjct: 61  TLSWLSSITNTSLVNKDLDVPELSINDLIDGPDNITEDSAKSVWVPNRNGVFINIAASFA 120

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
                E VI G    +   +PD   EFV A++   S   A  ++   PL+ +DK      
Sbjct: 121 ESHGCEYVIAGFNGEEAKTFPDNSKEFVNAVDECFSYSTANGVKVLAPLIDMDKVSIVRK 180

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQK 227
           A     L      + +CY+G + + CG C +C  R         + P +      K
Sbjct: 181 AME---LGAPLEYSWSCYHGAE-EPCGVCESCMRRKRAFLASGFEDPHIKKRADFK 232


>UniRef50_C0CIL2 Putative uncharacterized protein n=1 Tax=Blautia hydrogenotrophica
           DSM 10507 RepID=C0CIL2_9FIRM
          Length = 231

 Score =  213 bits (542), Expect = 4e-54,   Method: Composition-based stats.
 Identities = 68/228 (29%), Positives = 105/228 (46%), Gaps = 14/228 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++A+V+FSGG DSTTCL  A+++Y    V  ++  YGQ+H  E++ AR +    G    +
Sbjct: 3   EKAMVLFSGGVDSTTCLGLAIEKYGVQNVIPLSVQYGQKHEKEVEAARRILEYYGIEGME 62

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPN------TFVPGRNILFLTLAAIYAYQ 113
           +    +      S L+     +P+         N      T+VP RN LFL  AA  A  
Sbjct: 63  LDLTPIFAYSDCSLLSHSEKEIPEASYAEQLRENEGVPVSTYVPFRNGLFLACAASIALS 122

Query: 114 VKAEAVITGVCETDF--SGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
            +   +  G    D   S YPDC + F +++N AV  G  + IR E P + ++KA+  A 
Sbjct: 123 RECSCIYYGAHRDDAAGSAYPDCSESFFESMNRAVYEGSGQQIRIEAPFIKMNKAQIVAE 182

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
                +L +    T +CY G +   CG C  C  R+        + P 
Sbjct: 183 G---LRLKVPYEFTWSCYEGKE-KPCGVCGTCIDRSEAFKVNGVEDPA 226


>UniRef50_C4Z7K4 Queuosine biosynthesis protein QueC n=5 Tax=Bacteria
           RepID=C4Z7K4_EUBE2
          Length = 227

 Score =  212 bits (539), Expect = 9e-54,   Method: Composition-based stats.
 Identities = 65/230 (28%), Positives = 99/230 (43%), Gaps = 15/230 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +A+V+ SGG DSTT L  A+ +Y  D V  ++  YGQ+H  EI  A+ ++   G     +
Sbjct: 2   KALVLSSGGVDSTTALALAVSRYGKDNVIALSVSYGQKHDKEIQAAKAVSAFYGVEQLFL 61

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIP-------NTFVPGRNILFLTLAAIYAYQ 113
               +      S L + +  +P+                +T+VP RN LFL+ AA  A  
Sbjct: 62  DLSKIFQYSNCSLLQQSTEEIPEESYARQIEKTEGEKPVSTYVPFRNGLFLSSAASIALS 121

Query: 114 VKAEAVITGVCETDFS--GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
                ++ G    D +   YPDC   F  A+N A+  G    ++ E P + + KAE   +
Sbjct: 122 KDCSVILYGAHADDSAGFAYPDCSPMFNDAMNQAIFEGSGHQLKVEAPFVNMTKAEVVKM 181

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                +L+     T +CY G K   CG C  C  RA          P + 
Sbjct: 182 G---LELNAPYELTWSCYEG-KDVPCGKCGTCIDRAAAFAANGVSDPAIK 227


>UniRef50_A5ULR3 7-cyano-7-deazaguanine synthase n=5 Tax=Methanobacteriaceae
           RepID=QUEC_METS3
          Length = 227

 Score =  211 bits (537), Expect = 2e-53,   Method: Composition-based stats.
 Identities = 66/228 (28%), Positives = 116/228 (50%), Gaps = 14/228 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           K+A+ V SGG DS        +   E+H +TF+YGQ+  + E+  + E+  K+G   H+V
Sbjct: 4   KKAITVLSGGLDSCVATCVYAED-YEIHGITFNYGQKSFKQELKASEEICKKMGFT-HEV 61

Query: 61  LDVTLLNELAVSSLTRDSI-------PVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           +D+  LN+++ SSLT D+         + D +   D   + +VPGRNI+F ++A  YA  
Sbjct: 62  IDLPWLNKISNSSLTSDNEIPTLTHEELDDLDICEDTASSVWVPGRNIVFTSIATSYAES 121

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           + AE +I G  + + + +PD   EF+++ N+ +++G  ++I  + P + ++K E   L D
Sbjct: 122 IGAEIIIVGWDKEEAATFPDNSKEFLESFNNMLNVGSPQNIEIKAPAIDLNKDEIVKLGD 181

Query: 174 YYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                      + +CY G +   CG C +C  R           P+  
Sbjct: 182 EI---KAPMELSYSCYTGHE-KHCGVCESCMRRKRAFKLADVKDPSEY 225


>UniRef50_Q1IHK6 7-cyano-7-deazaguanine synthase n=9 Tax=Bacteria RepID=QUEC_ACIBL
          Length = 237

 Score =  211 bits (536), Expect = 2e-53,   Method: Composition-based stats.
 Identities = 78/226 (34%), Positives = 107/226 (47%), Gaps = 9/226 (3%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           +AVVV SGG DST C   A+++Y  + +  +   YGQR    E      +A +LG +   
Sbjct: 10  KAVVVLSGGMDSTVCATLAVREYGAENIGALHVSYGQRTAAREKQAFAAVAERLGIQTRL 69

Query: 60  VLDVTLLNELAVSSLTRDSIPVPD-YEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            ++      +  S+LT  +I VPD  E     IP T+VP RN   L +A  +A  + A  
Sbjct: 70  AVETPFFRAIGGSALTDANIAVPDAGELIGHEIPVTYVPFRNAHLLAMAVSWAEVLGASK 129

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGM-AKDIRFETPLMWIDKAETWALADYYGK 177
           +  G    D SGYPDCR EF +A N AV  G  A DI   TPL+ + K E  +L     +
Sbjct: 130 IYIGAVAQDSSGYPDCRPEFYEAYNLAVRRGTKAGDIEVVTPLIALRKHEIVSLG---LE 186

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           L    + T +CY   +   CG C +C LR        A  P   A 
Sbjct: 187 LGAPFDLTWSCY-SREDCACGVCDSCVLRLRAFEGAGAVDPVPYAP 231


>UniRef50_A6UV33 7-cyano-7-deazaguanine synthase n=12 Tax=Methanococcales
           RepID=QUEC_META3
          Length = 234

 Score =  209 bits (533), Expect = 5e-53,   Method: Composition-based stats.
 Identities = 63/232 (27%), Positives = 114/232 (49%), Gaps = 14/232 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           +AV V SGG DS  C+  A  +  E++ +TFDYGQR  + EI  +++L+   GA  HKV+
Sbjct: 2   KAVCVLSGGLDSAVCMAMAKSEGLELYSITFDYGQRAVKKEIIASKKLSELFGAV-HKVV 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDY--------EPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           ++  + + + S+LT+    VP          E   + +   +VP RN++  ++++ +A  
Sbjct: 61  ELPFVKDFSSSALTKKEEEVPTIKNSELDNLEKATETMEKVWVPARNMILFSISSGFAEF 120

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
           V A+ + TG+   + + +PD  +EF+   N  +  G    ++ ++PL    K E   +  
Sbjct: 121 VDAKYIYTGLNVEEGATFPDNTEEFLNRFNSVLEYGTLNKVKMKSPLYKYSKKEIVKIGK 180

Query: 174 YYGK-LDLV-RNETLTCYNGFKGD--GCGHCAACNLRANGLNHYLADKPTVM 221
              + L++     + +CY+  K D   CG C +C  R         + PT  
Sbjct: 181 KLEEKLNVEFLKYSYSCYHDNKTDFLHCGTCESCMRRKRAFKEAGVEDPTEY 232


>UniRef50_Q1CTN3 7-cyano-7-deazaguanine synthase n=12 Tax=Helicobacter
           RepID=QUEC_HELPH
          Length = 228

 Score =  208 bits (529), Expect = 1e-52,   Method: Composition-based stats.
 Identities = 85/216 (39%), Positives = 133/216 (61%), Gaps = 8/216 (3%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           VV FSGGQDSTT  V A +++ +V+ V FDY Q+H  E++ A+++A  L    ++++ + 
Sbjct: 7   VVSFSGGQDSTTLAVWAKKRFKKVYLVGFDYAQKHSVELECAQKIASLLQL-PYEIISLD 65

Query: 65  LLNELAVSSLTRDSIPVPDY-EPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            L  +  S+L ++S  +  +   +   +PN+FVP RN +F+TL   YA ++ A  +  GV
Sbjct: 66  FLENITRSALFKNSNDLMGHSHVQNKDLPNSFVPNRNAIFITLLHSYAQKIGASNIALGV 125

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
            + DFSGYPDC+++F+K++ HA++LG    I+  TPLM+++KA+ + +A   G LDLV  
Sbjct: 126 SQADFSGYPDCKEDFIKSIEHALNLGSNTAIKILTPLMFLNKAQEFQMAKDLGVLDLVIK 185

Query: 184 ETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
           ET TCY G +      G GC  C AC LR  G   +
Sbjct: 186 ETHTCYQGERKILHAYGYGCNECPACQLRKKGYEEF 221


>UniRef50_D1R557 Putative uncharacterized protein n=1 Tax=Parachlamydia
           acanthamoebae str. Hall's coccus RepID=D1R557_9CHLA
          Length = 221

 Score =  208 bits (529), Expect = 1e-52,   Method: Composition-based stats.
 Identities = 68/219 (31%), Positives = 98/219 (44%), Gaps = 13/219 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +A+V+ SGG DS   L +  +Q  E   ++FDYGQRHR E++ A+++A   G   H+V+ 
Sbjct: 2   KAIVLLSGGLDSAVMLARLHEQQIETLAISFDYGQRHRVELEFAKKIADFYGIE-HEVIP 60

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEA---DGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +  L     S+LT  + P      E      IP T+VP RN LFL  A   A    A+ +
Sbjct: 61  IDPLIFRGGSALTSSATPQKGKSKEDILKQSIPFTYVPARNTLFLAYALAKAEMHGADQI 120

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR-----FETPLMWIDKAETWALADY 174
             G    DF  YPDCR  + +A     +    + +        TPL+   K E       
Sbjct: 121 HFGSNAMDFLSYPDCRPGYFEAAQELFNRATKQSVEGKAPQIVTPLLKWTKEEIVKEGYR 180

Query: 175 YGKLDLVRNETLTCYNGF-KGDGCGHCAACNLRANGLNH 212
              L +    T +CY+    G  C  C AC LR +    
Sbjct: 181 ---LKVPFELTSSCYDPTANGQPCQQCDACTLRQDAFLS 216


>UniRef50_A6DLD5 ExsB protein n=1 Tax=Lentisphaera araneosa HTCC2155
           RepID=A6DLD5_9BACT
          Length = 226

 Score =  207 bits (528), Expect = 2e-52,   Method: Composition-based stats.
 Identities = 73/230 (31%), Positives = 111/230 (48%), Gaps = 12/230 (5%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKL-GARA 57
           MK+A V+ SGG DSTT L   ++   YDEV+ ++FDYGQ+H  E+ VA+     L   + 
Sbjct: 1   MKKAAVLISGGLDSTTLLHHVIKSLGYDEVYALSFDYGQKHSRELIVAKAQCDSLPEVKE 60

Query: 58  HKVLDVTLLNELAVS--SLTRDSIPVPDYEPEADG---IPNTFVPGRNILFLTLAAIYAY 112
           H VLD++ + +   S  +L +  + VPD +  A+G    P T+VP RN++ L++AA +A 
Sbjct: 61  HNVLDISYMGDFLGSSSALVKGGVEVPDLKDIAEGDLDQPVTYVPNRNMMLLSIAASFAE 120

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 +  G    D  GY DC + F+  +N   SL     +  E P + + K     + 
Sbjct: 121 SRDCRQLYYGAQAQDEYGYWDCTESFLARMNDVFSLNRRTGVHVEAPFVNMSKGHVAKIG 180

Query: 173 DYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                L +   +T TCY G     C  C +C  RA        D P +  
Sbjct: 181 ---LALGIDYAQTWTCYRGG-DKPCEVCPSCVERALAFKECGIDDPLLKK 226


>UniRef50_Q7NCE2 7-cyano-7-deazaguanine synthase n=2 Tax=Bacteria RepID=QUEC_GLOVI
          Length = 234

 Score =  207 bits (526), Expect = 3e-52,   Method: Composition-based stats.
 Identities = 99/226 (43%), Positives = 127/226 (56%), Gaps = 9/226 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+A+VVFSGGQDSTTC   A ++YDEVH VTF+Y QRH  E++ AR +   LG   H+ 
Sbjct: 1   MKKALVVFSGGQDSTTCAALACREYDEVHAVTFEYNQRHAIELESARAVGQALGLTGHEF 60

Query: 61  LDVTLLNELAVSSLTRDS--IPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           + +  L     S L  D+             G+  TFVPGRNILFLTLAA  A+ +    
Sbjct: 61  IRLGPL-LKGTSPLVSDAPLGQYASAAELPAGVEPTFVPGRNILFLTLAANRAFCLGTGD 119

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKD---IRFETPLMWIDKAETWALADYY 175
           ++ GVCE DF+GY DCR  FV+A+  A+  G+  D   IR  TPLM + KAET  L+   
Sbjct: 120 IVIGVCEADFAGYWDCRQVFVEAMARALGEGIYGDANAIRIHTPLMRLTKAETVKLSVEV 179

Query: 176 G--KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
              + + V   + TCY G +G GCG C AC LR  G        P 
Sbjct: 180 LGERFEEVLALSHTCYAGVRG-GCGRCHACILRDRGFREAGVPDPI 224


>UniRef50_UPI0001AF28A8 succinoglycan biosynthesis regulator n=2 Tax=Streptomyces
           filamentosus RepID=UPI0001AF28A8
          Length = 223

 Score =  206 bits (523), Expect = 6e-52,   Method: Composition-based stats.
 Identities = 73/225 (32%), Positives = 100/225 (44%), Gaps = 9/225 (4%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M R + + SGG DS         +  EVH ++ DYGQRHR E D AR  A ++GA    V
Sbjct: 1   MARVIAIVSGGLDSVVLAHHLHAEGHEVHLLSIDYGQRHRKEHDYARIAADRIGAEHEIV 60

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
               + + L   SLT   + VPD    A G  N  VP RN++ L++A   A  VKA+ V 
Sbjct: 61  DLSNVASMLPGYSLTDSQVDVPDQHYTAPGSVNV-VPNRNVILLSVAYGKAAAVKADQVA 119

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAK----DIRFETPLMWIDKAETWALADYYG 176
            G    D S  PDC  EF+ + N    +  A      +    P + + K +   L +   
Sbjct: 120 IGALAGDASTAPDCSAEFISSFNAMEKIACAGYAPAHLEVVAPFIDLHKHDVIRLGEK-- 177

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            L +   ET +C+ G +   CG CAAC  R            TV 
Sbjct: 178 -LGVDWTETWSCFKGTEV-HCGTCAACVDRIQAFERAETTDTTVY 220


>UniRef50_A6VQW6 7-cyano-7-deazaguanine synthase n=22 Tax=Pasteurellaceae
           RepID=QUEC_ACTSZ
          Length = 235

 Score =  205 bits (522), Expect = 8e-52,   Method: Composition-based stats.
 Identities = 107/225 (47%), Positives = 143/225 (63%), Gaps = 8/225 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           ++AVV+FSGGQDSTTCL+QA++ Y    V  VTF YGQRH  E+  A+ +A  LG +   
Sbjct: 10  RKAVVIFSGGQDSTTCLLQAIRDYGPNNVEAVTFQYGQRHAIELQKAQWIAQDLGVKQTL 69

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           + D +++  +  +++  D+      + + +G+PNTFV GRN LFL  AAIYA       +
Sbjct: 70  I-DTSVIKTITTNAMMSDA----QIKQDENGMPNTFVDGRNALFLLYAAIYAKGQGITDI 124

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           ITGVCETDFSGYPDCR+ F+K++N  ++L M       TPLM++ KA+TW LAD  G LD
Sbjct: 125 ITGVCETDFSGYPDCRNVFIKSMNVTLNLAMDYQFNIRTPLMYLTKAQTWQLADELGALD 184

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
            VR  T TCY G +G GCG C +C LR  GL  YLA K  V  + 
Sbjct: 185 YVRQYTHTCYLGVEG-GCGTCPSCLLREKGLKQYLAQKSAVEKSS 228


>UniRef50_UPI0001C418E0 queuosine biosynthesis protein QueC n=1 Tax=Methanobrevibacter
           ruminantium M1 RepID=UPI0001C418E0
          Length = 211

 Score =  205 bits (521), Expect = 1e-51,   Method: Composition-based stats.
 Identities = 65/215 (30%), Positives = 97/215 (45%), Gaps = 10/215 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K  V++ SGG DSTT L + L + + V  ++F+YGQ+   EI+ A E+  K+    H V 
Sbjct: 3   KDVVLILSGGLDSTTLLYKLLNEGNNVTALSFNYGQKAAIEIERASEIC-KMNDVNHFVF 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+  + +L  SSLT                 +T VP RN + L LA  +A     E V  
Sbjct: 62  DIRNIVDLLSSSLTDKDND------NVQEPKSTVVPSRNTILLELATAFAISNNLEEVYY 115

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G  + D   YPD   EF+K +N    +   + I    P +  DK +   LA    +L + 
Sbjct: 116 GAIKADTGDYPDTTPEFLKQINELNKVNNYEYIPVCAPFIDTDKKDVVKLA---LELGVP 172

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
             +T +CY    G  CG C +C  R   +     +
Sbjct: 173 IEKTWSCYVNNDGTPCGECFSCKSRIEAIKEAKEE 207


>UniRef50_A5A3S4 BcepGomrgp35 n=1 Tax=Burkholderia phage BcepGomr RepID=A5A3S4_9CAUD
          Length = 301

 Score =  205 bits (521), Expect = 1e-51,   Method: Composition-based stats.
 Identities = 98/230 (42%), Positives = 131/230 (56%), Gaps = 18/230 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K AV+VFSGGQDSTT L +A+ +   V  + F YGQ+H  E++ A ++    G    K++
Sbjct: 4   KTAVIVFSGGQDSTTILGKAIAEGYRVFPIAFTYGQKHAIELEQAAKIVDHFGLAGLKIV 63

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D++ L E+  S+L      V         +P +FVP RN  FLTLA  YA QV A+ + T
Sbjct: 64  DLSALGEVTTSALIGTDGDVNKPSDANPSLPASFVPNRNAAFLTLAHAYAQQVGADELWT 123

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           GVC TD+SGYPDCRD F+ AL   +++G    I  +TPLM+IDKAET+ +A+  G LD+V
Sbjct: 124 GVCMTDYSGYPDCRDSFINALAATLNIGYDTSITIKTPLMYIDKAETFRMAELAGCLDVV 183

Query: 182 RNETLTCYNGFK------------------GDGCGHCAACNLRANGLNHY 213
              + TCYNG +                  G GCG C AC LRA G   Y
Sbjct: 184 IEMSHTCYNGVRSNGEVPTNPSEGGGFFDWGHGCGECPACKLRAGGWREY 233


>UniRef50_A6LIS9 7-cyano-7-deazaguanine synthase n=88 Tax=Bacteria RepID=QUEC_PARD8
          Length = 224

 Score =  204 bits (520), Expect = 1e-51,   Method: Composition-based stats.
 Identities = 104/213 (48%), Positives = 130/213 (61%), Gaps = 1/213 (0%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A+V FSGGQDSTTCL  A + +  V  V F YGQ+H  EI+VAR++A         +   
Sbjct: 7   ALVCFSGGQDSTTCLFWAKKHFSRVEAVCFTYGQKHSLEIEVARKIAADADVPFQLLDVS 66

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            +       SLT  SI +   +P  D  PNTFVPGRN++FLT AAI A       ++TGV
Sbjct: 67  LISQLDPNCSLTNASIEMDQEKP-EDSYPNTFVPGRNMVFLTFAAILARGKGIYHLVTGV 125

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
            E D+SGYPDCRD FV++LN  ++L M +     TPLM  DK+E W L+D  G  DLVR 
Sbjct: 126 SEADYSGYPDCRDTFVRSLNVTLNLAMDEQFVIHTPLMDRDKSEVWELSDELGVFDLVRT 185

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
           +TLTCYNG   +GCGHC AC LR +GL  YL  
Sbjct: 186 QTLTCYNGVMAEGCGHCPACKLRKDGLEKYLKR 218


>UniRef50_Q7M8H4 7-cyano-7-deazaguanine synthase n=2 Tax=Campylobacterales
           RepID=QUEC_WOLSU
          Length = 233

 Score =  204 bits (520), Expect = 2e-51,   Method: Composition-based stats.
 Identities = 99/217 (45%), Positives = 130/217 (59%), Gaps = 8/217 (3%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A+VVFSGGQDSTTCL  A++ +  V  +TFDYGQRHR EI+ +R +A KLG +   +L+ 
Sbjct: 14  ALVVFSGGQDSTTCLGWAIKHFKSVEAITFDYGQRHRIEIEQSRLIAQKLGIKQF-ILEF 72

Query: 64  TLLNELAVSSLTRDSIPVP-DYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
            L ++L  S+L   S  +          +P +FVP RN LF TLA  YA +     +ITG
Sbjct: 73  DLFSKLGDSALLETSSDLKASPHRTKPHLPASFVPNRNALFFTLAHAYAQREGLTHIITG 132

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           + E D+SGYPDCR+EF+  L   ++LG    I F  PL+  +KA+T+ALA   G LDLV 
Sbjct: 133 ISEADYSGYPDCREEFITHLERTLNLGSDSSILFAYPLLHRNKAQTFALAKECGVLDLVL 192

Query: 183 NETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
            E+ TCYNG +      G GC  C AC LR  G   Y
Sbjct: 193 EESHTCYNGDRSHRYEWGYGCKECPACGLREKGWREY 229


>UniRef50_Q857D1 Gp3 n=2 Tax=unclassified Siphoviridae RepID=Q857D1_9CAUD
          Length = 235

 Score =  204 bits (519), Expect = 2e-51,   Method: Composition-based stats.
 Identities = 72/225 (32%), Positives = 102/225 (45%), Gaps = 10/225 (4%)

Query: 5   VVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +V+ SGG DS+T L  AL +   D+   +  DYGQRH  E   ++ +A   GA   ++ D
Sbjct: 8   LVLLSGGMDSSTLLAWALDRPDVDQASALFVDYGQRHVVERASSQLVAQHYGAAWSEL-D 66

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +        S+LT  +  VP      D +  T VP RN   ++ AA  A  +  +AV+T 
Sbjct: 67  LRAFGASVNSALTVMAREVPHGHYAEDNMVQTVVPNRNATLISAAAGIAASLGHDAVLTA 126

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           V   D + YPDCR EFV A++ A   G    +    P    DKA+   L    G+L +  
Sbjct: 127 VHVGDHAVYPDCRPEFVAAIDMATFAGC--GVHVVAPFQTGDKADIARLG---GQLGVPY 181

Query: 183 NETLTCYNGF--KGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
           + T +CY G       CG C  C  RA          PT  A  +
Sbjct: 182 HLTWSCYEGGLNDARHCGRCGTCVERAEAFAEAGVPDPTEYADAE 226


>UniRef50_D0MDN1 ExsB protein n=1 Tax=Rhodothermus marinus DSM 4252
           RepID=D0MDN1_RHOM4
          Length = 238

 Score =  203 bits (517), Expect = 4e-51,   Method: Composition-based stats.
 Identities = 94/221 (42%), Positives = 124/221 (56%), Gaps = 9/221 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + A+V+ SGGQDSTTCL  AL  + +V  + F YGQ+H  E++ AR++A + G       
Sbjct: 17  ETALVLLSGGQDSTTCLYWALHYFPKVEALGFHYGQKHAVELEQARKIADRAGVP---FT 73

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            + L   L  S+LT     V    P A  +P  FVPGRN LFLTLAA Y +      ++ 
Sbjct: 74  VLDLRGLLRGSALTEHEQDVSAAHPLAPHLPAAFVPGRNALFLTLAASYGFTRGIHDLVG 133

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+C+TD+SGYPDCR EF+ A+  A+SL +   IR  TPLM + KAETW LA   G LD++
Sbjct: 134 GMCQTDYSGYPDCRREFIDAMERALSLALDTPIRIHTPLMHLTKAETWRLARELGILDVI 193

Query: 182 RNETLTCYNGFKGD----GCGHC--AACNLRANGLNHYLAD 216
              T T YNG + +    G G     A  LRA G     A 
Sbjct: 194 VELTHTDYNGDRSERHPWGYGRLDNPASILRARGYEEAKAR 234


>UniRef50_B0TFM0 Exsb protein, putative n=1 Tax=Heliobacterium modesticaldum Ice1
           RepID=B0TFM0_HELMI
          Length = 247

 Score =  202 bits (515), Expect = 6e-51,   Method: Composition-based stats.
 Identities = 82/246 (33%), Positives = 117/246 (47%), Gaps = 29/246 (11%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +RAVV+ SGG DSTTC+  A  + +++  +TF Y QRH  EI+ A+ +A   GA  H+++
Sbjct: 6   ERAVVILSGGLDSTTCMALADCRGEDIFALTFAYNQRHIREIEAAQAVACHYGA-HHQII 64

Query: 62  DVTLLNELAVSSLTRDSIPV-------------------PDYEPEADGIPNTFVPGRNIL 102
           D+  +   +  + T     V                    +    A  IP T+VP RNIL
Sbjct: 65  DLGAVFAASALTSTEIRPEVAAPLGGSAVAKMEAMGCAEKNPVAIATAIPATYVPARNIL 124

Query: 103 FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFE 157
           FL  A  +A +V A  + TGV   D  GYPDCR EF+ A    + +G       + +   
Sbjct: 125 FLAYALSFAEKVGARRIYTGVNALDNPGYPDCRPEFIAAFQRMIDVGTKAGTEGRSVEVF 184

Query: 158 TPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADK 217
           TPL+ ++KA+   LA     LD   + TLTCY G +   CG C +C LR  G        
Sbjct: 185 TPLIEMNKADIIRLALD---LDAPLHLTLTCYYGERP-ACGECNSCRLRLEGFRLLGKGD 240

Query: 218 PTVMAA 223
           P     
Sbjct: 241 PIAYRK 246


>UniRef50_D2RGV6 ExsB protein n=2 Tax=Archaeoglobaceae RepID=D2RGV6_ARCPR
          Length = 233

 Score =  202 bits (514), Expect = 7e-51,   Method: Composition-based stats.
 Identities = 65/216 (30%), Positives = 103/216 (47%), Gaps = 8/216 (3%)

Query: 8   FSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVAREL---ALKLGARAHKVLDVT 64
            SGG DS+T L   +++YDE++ +T+ YGQRH  E++ A+ +   A +     H++ D++
Sbjct: 1   MSGGIDSSTLLWYLMKRYDEIYVITYIYGQRHAREVEHAKMIVEKAREKAKIHHEIADIS 60

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
            +  L        +  VP+     +    T VP RN++ L +A  YA +V    V     
Sbjct: 61  CIQRLIAKGALTGNEEVPEGFYTDESQKKTIVPNRNMILLAIAVGYAVKVGEREVYYAAH 120

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMA-KDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
           ++D++ YPDCR EFVKAL+ AV L      +  + P + + KA+   L     KL +   
Sbjct: 121 KSDYATYPDCRKEFVKALDTAVYLATIFDPVVIKAPFVDMTKADIVKLG---LKLGVPYE 177

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
            T +CY G +   C  C  C  R           P 
Sbjct: 178 LTWSCYRGEE-RPCLRCGTCLERTEAFLINGVKDPA 212


>UniRef50_B9L187 ExsB protein n=1 Tax=Thermomicrobium roseum DSM 5159
           RepID=B9L187_THERP
          Length = 226

 Score =  202 bits (513), Expect = 1e-50,   Method: Composition-based stats.
 Identities = 76/222 (34%), Positives = 107/222 (48%), Gaps = 14/222 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RAVV+ SGG DSTT           ++ ++F YGQRH  E++ AR +A KL    H+V+ 
Sbjct: 9   RAVVLLSGGLDSTTLAYLLDAYGFVLYGLSFRYGQRHERELEAARAIAEKLRFAEHRVVT 68

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           +  L +   SSL               GIP+T+VP RN++FL +A  YA  + A+AV   
Sbjct: 69  ID-LAQWGGSSLVGSGEIPRQPT---SGIPSTYVPVRNLVFLAIAHAYAEAIDADAVAIA 124

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLG------MAKDIRFETPLMWIDKAETWALADYYG 176
           V + D+SGYPDCR EF+ A   A  L         + I    P + + K+    L     
Sbjct: 125 VSQVDYSGYPDCRGEFLAAYQQAADLASRRFVETGRRIPVLAPFLDVPKSAIVRLG---L 181

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
           +L +   ET +CY G +   C  C +C LRA          P
Sbjct: 182 RLGVDYAETWSCYRGGE-RPCQECDSCRLRAQAFAEAGVPDP 222


>UniRef50_Q981C9 7-cyano-7-deazaguanine synthase n=13 Tax=Thermoprotei
           RepID=QUEC_SULSO
          Length = 464

 Score =  200 bits (509), Expect = 2e-50,   Method: Composition-based stats.
 Identities = 54/224 (24%), Positives = 94/224 (41%), Gaps = 10/224 (4%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVL 61
           R +VV SGG DST    + L++  EV  + F+Y  +    E +  R++A  L     ++ 
Sbjct: 243 RVLVVASGGLDSTVAATKLLREGHEVTLIHFNYHHKAEEKEREAVRKIAEYLQIPLLEI- 301

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGI--PNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +  L   +  ++L +    +       +G    + +VP RN++F +++   A     +A+
Sbjct: 302 NTDLFKIIGHATLIKGGGEIVKDRKGEEGAEFAHEWVPARNLIFFSVSLAIAEAYGYDAI 361

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHA--VSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
            +G+   +   YPD   EF++ LN     + G  K I    P+  + K E   L    G 
Sbjct: 362 ASGINLEEAGAYPDNEMEFIRMLNKLSPYATGPNKRIEILMPVGNLVKHEIVKLGYEIG- 420

Query: 178 LDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                + T +CY G +   CG C  C +R           P   
Sbjct: 421 --APLHLTWSCYEGGQ-KHCGKCGPCYMRKMAFRINGLKDPVEY 461


>UniRef50_Q8UAM7 7-cyano-7-deazaguanine synthase n=71 Tax=Alphaproteobacteria
           RepID=QUEC_AGRT5
          Length = 236

 Score =  199 bits (506), Expect = 6e-50,   Method: Composition-based stats.
 Identities = 73/225 (32%), Positives = 104/225 (46%), Gaps = 8/225 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK  V+  SGG DS +   +   +++ +  V+FDYGQRH+ E+D A + A +LG   H +
Sbjct: 1   MKTIVI-CSGGLDSVSLAHKIAAEHELLALVSFDYGQRHKKELDFAADCAKRLGVPHHLI 59

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
              T+   L  S+LT D I VPD     + + +T VP RN + LT+A   A   +A+AV 
Sbjct: 60  DIGTIGAHLTGSALTDD-IDVPDGHYAEETMRSTVVPNRNAIMLTIAFGLAAAQQADAVA 118

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAK--DIRFETPLMWIDKAETWALADYYGKL 178
             V   D   YPDCR  F+ + N   +  +    D+    P + + KA     A  YG  
Sbjct: 119 IAVHGGDHFIYPDCRPGFIGSFNAMQAHALEGYADVTLFAPYVTVSKAAIVTDAVKYGT- 177

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
                ET +CY G     CG C  C  R    +      PT    
Sbjct: 178 --PFGETWSCYKGG-LRHCGRCGTCVERREAFHLAGVTDPTEYED 219


>UniRef50_Q5JHU5 7-cyano-7-deazaguanine synthase n=6 Tax=Thermococcus
           RepID=QUEC_PYRKO
          Length = 239

 Score =  199 bits (506), Expect = 6e-50,   Method: Composition-based stats.
 Identities = 65/237 (27%), Positives = 103/237 (43%), Gaps = 18/237 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MKRAVV+FSGG DST CL  A + YDEV  +T +YG       +   E   K      K+
Sbjct: 1   MKRAVVLFSGGLDSTACLYWAKKNYDEVIMLTVNYGSNEEKVTNKVAEFFSKELDVPLKI 60

Query: 61  LDVTLLNEL---AVSSLTRDSIP------VPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           + +  L E      ++L     P      + + E   +   + +VP RN++ + +AA   
Sbjct: 61  VRLDFLEEFSKLRGTTLVGGETPKVTGEELENIEVAQETAKSVWVPARNVVLIAVAASLL 120

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             +    ++ G    + + +PD   EFV+ +N  +  G   ++R   PL+ +DK     L
Sbjct: 121 DALGGGDIVVGFNAEEGATFPDNTPEFVEKMNEMLKYGTMAEVRVVAPLINLDKRGIAKL 180

Query: 172 ADYYGKLDLVRNETLTCYNG------FKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                +L      + +CY         K   CG C +C  R  GL   + +  TV A
Sbjct: 181 ---LKELGAKYEYSNSCYMPKGFTEDGKPIHCGECESCVRRHRGLIEGIGEDKTVYA 234


>UniRef50_A9A531 ExsB family protein n=2 Tax=Thaumarchaeota RepID=A9A531_NITMS
          Length = 224

 Score =  197 bits (502), Expect = 2e-49,   Method: Composition-based stats.
 Identities = 75/232 (32%), Positives = 105/232 (45%), Gaps = 21/232 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           MK+AVVVFSGG DS  C V  L+   E++ +TF YGQ+   EI   +  A KLG + HK+
Sbjct: 1   MKKAVVVFSGGVDS-VCAVSFLKSKYELYGITFSYGQKANMEISATKSFAKKLGLKQHKI 59

Query: 61  LDVTLLNELAVSS--LTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +D+  + EL   S  LT     +P          +  VP RN +FL++A+ +AY + A  
Sbjct: 60  IDIGFMKELYGDSNVLTSSKRKIPSKFEY-----SIVVPIRNAVFLSVASAWAYTLNASL 114

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLG--------MAKDIRFETPLM-WIDKAETW 169
           V+ G    D   YPDCR  F K L  A + G        + K I+  +P    + K++  
Sbjct: 115 VVYGAHTGD-KYYPDCRPAFAKKLESAFNQGEIDGINSKIRKKIKIWSPYREGLSKSDLL 173

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
                   L      T +CY   K   CG C +CN R            T  
Sbjct: 174 KKGKKV--LGDSIFRTWSCY-SNKKLHCGVCESCNNRKIAFEKAGVTDKTKY 222


>UniRef50_C7ML00 ExsB protein n=9 Tax=Bacteria RepID=C7ML00_CRYCD
          Length = 240

 Score =  196 bits (498), Expect = 6e-49,   Method: Composition-based stats.
 Identities = 76/231 (32%), Positives = 109/231 (47%), Gaps = 17/231 (7%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDE--VHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           A+V+ SGG DSTTCL  A+ ++ +  V  V+F YGQRH  E+D A  +A   G   H VL
Sbjct: 15  ALVLSSGGVDSTTCLALAVDRFGQANVSTVSFFYGQRHSRELDAAAAVAEHYGV-HHYVL 73

Query: 62  DVTLLNELAVSSLTRDSIPVPDY-----EPEADGIPNTFVPGRNILFLTLAAIYA---YQ 113
           D+  +   + ++L   S     +     + E DG PNT+VP RN L L+ AA  A   + 
Sbjct: 74  DLASVMHYSNNALMATSTQHVPHGSYAQQMEDDGHPNTYVPFRNGLMLSAAAALAASLFP 133

Query: 114 VKAEAVITGVCETDFS--GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             + A+  G    D +   YPDC   F   +  A+S G   ++R ETP +  +KA+  A 
Sbjct: 134 DTSCALYLGAHADDAAGNAYPDCSPAFTAHMGRAISFGTYGNVRLETPFVNQNKAQVVAE 193

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                +L +    T +CY G +   CG CA C  R           P    
Sbjct: 194 G---LRLGVPYEMTWSCYEGGE-KPCGTCATCLDRIAAFEANGVKDPVSYR 240


>UniRef50_D2RLP8 ExsB protein n=3 Tax=Bacteria RepID=D2RLP8_ACIFE
          Length = 232

 Score =  194 bits (493), Expect = 2e-48,   Method: Composition-based stats.
 Identities = 68/232 (29%), Positives = 103/232 (44%), Gaps = 19/232 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+A+V+ SGG DSTTC+  A++++  + V  V+  YGQ+HR E++ AR++A   G   H 
Sbjct: 3   KKALVLSSGGVDSTTCVSVAVEEFGKENVSTVSIFYGQKHRKELECARKVAEYYGL-PHY 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIP-------NTFVPGRNILFLTLAAIYA- 111
            LD++ + + +  SL   S     +E     I        +T+VP RN L L+  A  A 
Sbjct: 62  ELDLSKIFQYSNCSLLDHSTEEIVHESYEQQIAENGEGKVSTYVPFRNGLMLSAVASLAM 121

Query: 112 --YQVKAEAVITGVCETDFS--GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
             Y+    ++  G    D +   Y DC + F+ A+  A+SLG         P     KA 
Sbjct: 122 SIYEKDDVSIFIGAHADDAAGNAYADCSEAFIGAMGKAISLGTYNQCHIVAPFASCSKAG 181

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
                    +L    + T +CY G     CG C  C  RA          P 
Sbjct: 182 VVKRG---LELHTPYDLTWSCYEGG-DKPCGTCGTCIDRARAFAANGVKDPA 229


>UniRef50_Q8U4N3 7-cyano-7-deazaguanine synthase n=3 Tax=Pyrococcus RepID=QUEC_PYRFU
          Length = 240

 Score =  193 bits (491), Expect = 3e-48,   Method: Composition-based stats.
 Identities = 70/238 (29%), Positives = 110/238 (46%), Gaps = 19/238 (7%)

Query: 1   MKR-AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAH 58
           MKR AVV+FSGG DST CL  A +QYDEV  +T +YG         VA   + +L  R  
Sbjct: 1   MKRRAVVLFSGGLDSTACLYWAKKQYDEVIMLTVNYGSNEERVTNKVAEYFSKELDVRLK 60

Query: 59  KV--LDVTLLNELAVSSLTRDSIP------VPDYEPEADGIPNTFVPGRNILFLTLAAIY 110
            V    +   +E+  SSL    +P      + D E  ++   + ++P RN++ +++AA  
Sbjct: 61  IVKLDFLKEFSEIRGSSLVGGEVPRVTAEELEDIEKASETARSVWIPARNLVLISVAASL 120

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
              +    +I G    + + +PD   EFV+ LN A+       ++   PL+ +DK     
Sbjct: 121 LDALGGGDIIVGFNAEEATTFPDNSREFVEKLNEALRFATLNPVKVVAPLIDLDKRGIAK 180

Query: 171 LADYYGKLDLVRNETLTCYNG------FKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           L     +L+     + +CYN        +   CG C +C  R  GL   + +  TV A
Sbjct: 181 L---LKELNAKYEYSNSCYNPKGFTEDGRPIHCGECESCVRRHKGLIEGIGEDKTVYA 235


>UniRef50_B3T674 Putative ExsB n=1 Tax=uncultured marine crenarchaeote
           HF4000_ANIW141O9 RepID=B3T674_9ARCH
          Length = 233

 Score =  191 bits (486), Expect = 1e-47,   Method: Composition-based stats.
 Identities = 72/232 (31%), Positives = 107/232 (46%), Gaps = 21/232 (9%)

Query: 1   MKRAVVVFSGGQDS-TTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           MK+AVVVFSGG DS +TC     +Q  E++ ++F YGQ+   EI  A+  A  LG + HK
Sbjct: 10  MKKAVVVFSGGIDSVSTCTYL--KQKYELYGISFLYGQKANQEIKKAKSFAKILGLKEHK 67

Query: 60  VLDVTLLNELAV--SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           V+D+  + +L    ++LT     +P     +    +  VP RN +FL++A  +A+ + A 
Sbjct: 68  VVDINFMKKLYGDTNALTSSKKKIP-----SKFNYSVVVPIRNGIFLSIATAWAFSINAS 122

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSL--------GMAKDIRFETPLMWIDKAETW 169
            V  G    D   YPDCR  F K +  A +         G+ K I+  +P      ++  
Sbjct: 123 LVAYGAHMGD-KNYPDCRPAFSKKIEFAFNQGEIDGIKSGIRKRIQIWSPYKAKL-SKKQ 180

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            L   Y  L      T +CY   K   CG C +CN R         +  T  
Sbjct: 181 LLKKGYDTLGDKIFNTWSCYKNGKV-QCGKCESCNNRKAVFLEAGIEDKTPY 231


>UniRef50_C0WAE7 Archaeosine biosynthesis protein queC n=3 Tax=Bacteria
           RepID=C0WAE7_9FIRM
          Length = 233

 Score =  191 bits (485), Expect = 2e-47,   Method: Composition-based stats.
 Identities = 66/232 (28%), Positives = 101/232 (43%), Gaps = 19/232 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQ--YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+A+V+ SGG DSTTC+  A+ +   + V  V+  YGQ+HR E+D A+++A       H 
Sbjct: 3   KKALVLSSGGVDSTTCVSVAVHELGRENVATVSIFYGQKHRKELDAAQKVADFYHV-PHY 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIP-------NTFVPGRNILFLTLAAIYA- 111
            LD++ + + +  SL   S     +E   D I        +T+VP RN L L+  A  A 
Sbjct: 62  ELDLSKIFQYSNCSLLSHSTEEIIHESYEDQIAQNGEGKVSTYVPFRNGLMLSAVASLAM 121

Query: 112 --YQVKAEAVITGVCETDFS--GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
             ++     +  G    D +   Y DC + F  A+  A+S+G  +      P + ++KA 
Sbjct: 122 SLFEKDDVDIYLGAHADDAAGNAYADCSEAFTDAMGKAISIGTYEQCHLVAPFVEVNKAG 181

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPT 219
                   G        T +CY G     CG C  C  RA          P 
Sbjct: 182 IVKKGLELGT---PYELTWSCYEGG-DRPCGTCGTCIDRARAFAANGVPDPA 229


>UniRef50_Q0W7U0 Succinoglycan biosynthesis regulator n=1 Tax=uncultured
           methanogenic archaeon RC-I RepID=Q0W7U0_UNCMA
          Length = 222

 Score =  191 bits (484), Expect = 2e-47,   Method: Composition-based stats.
 Identities = 50/222 (22%), Positives = 94/222 (42%), Gaps = 15/222 (6%)

Query: 7   VFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKVLDVTL 65
           + S G DS     +   ++     +TFDYGQR    EI++AR++  +   R H+V+++  
Sbjct: 1   MLSSGLDSVVAFKKEHDEHGVALAITFDYGQRAASKEIEMARQICARYHVR-HEVIELPW 59

Query: 66  LNELAVSSLTRDSIPVPDYEPEA---------DGIPNTFVPGRNILFLTLAAIYAYQVKA 116
           L ++  ++L      VP+              +     +VP RN  F+ +AA +      
Sbjct: 60  LKQITRTALVSRQAEVPEPTEAELDEIKGRALETAHLVWVPNRNGAFINIAAAFGDAYGY 119

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
             ++ G    + + +PD    +V A+N +++L   K ++   P++  DK E         
Sbjct: 120 NRIVCGFNAEEGATFPDNTPAYVDAVNKSLALSCEKQVQVVAPVIHFDKEEIIREGVRI- 178

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
             +   + + +CY   +   CG C +C  RA          P
Sbjct: 179 --EAPLDLSWSCYFAGE-KPCGKCESCMRRARAFKRAGVPDP 217


>UniRef50_Q6WI28 ExsB protein n=1 Tax=Vibrio phage KVP40 RepID=Q6WI28_BPKV4
          Length = 238

 Score =  191 bits (484), Expect = 2e-47,   Method: Composition-based stats.
 Identities = 64/227 (28%), Positives = 107/227 (47%), Gaps = 11/227 (4%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           + V V SGG DST    + + ++  D V  ++F YGQ+   E++ A+    KLG   H++
Sbjct: 2   KVVSVLSGGLDSTILTYKLVDEFGKDNVFALSFYYGQKQSIELEKAKLTCQKLGIE-HRL 60

Query: 61  LDVTLLNELA---VSSLTRDSIPVPDYEPE-ADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
           +D++ L E+     +++    + +P  +    D  P T VP RN+L  T+A  +A     
Sbjct: 61  IDISFLGEVVAPVCANIAGSDVAMPTIQDVLGDPQPPTEVPYRNMLLNTIAFSFAQANGC 120

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            AV TG+   D  GY DC  +F+  +N   +L    +I    P   + KA+  A+     
Sbjct: 121 SAVFTGIQVHDAYGYWDCTQDFLDGMNKISALNRQHEITLHAPFAQMSKADEIAIGVE-- 178

Query: 177 KLDLVRNETLTCYNGFK-GDGCGHCAACNLRANGLNHYLADKPTVMA 222
            L +   ++LTCY+  + G+ CG C +C  R           P   +
Sbjct: 179 -LGVPFEDSLTCYDPNEAGESCGKCPSCAERIMNFGKAGIVDPAPYS 224


>UniRef50_C6XGK1 ExsB protein n=9 Tax=Bacteria RepID=C6XGK1_LIBAP
          Length = 240

 Score =  190 bits (483), Expect = 3e-47,   Method: Composition-based stats.
 Identities = 91/229 (39%), Positives = 135/229 (58%), Gaps = 22/229 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKL---------G 54
           A+++FSGGQDS+TCL  AL ++D V  ++FDYGQR++ E++    +  K+          
Sbjct: 11  ALLLFSGGQDSSTCLSWALDRFDRVETLSFDYGQRNKVELECRLCVRKKIVELMPKWKDS 70

Query: 55  ARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
                +L + +L +++ SSLT++       + + + +PNTFVPGRNI+FL  AA  AY++
Sbjct: 71  LGEDHILPLAILGDISHSSLTKNV----AMKIQDNNLPNTFVPGRNIIFLVFAATLAYRL 126

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADY 174
               ++ GVCETD+SGYPDCR + ++A+  A++LGM   +   TPLMW+ K ETW LA  
Sbjct: 127 GITNIVIGVCETDYSGYPDCRHDTIRAIETAINLGMESHVTVHTPLMWLKKYETWKLAQD 186

Query: 175 YGKLD---LVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHYL 214
            G  D   L+  E+ TCY G +      G GC  C AC LR  G   + 
Sbjct: 187 IGGQDLVNLILEESHTCYLGKRDKRYEWGYGCNSCPACYLRQKGWMEFK 235


>UniRef50_B4E7W5 7-cyano-7-deazaguanine synthase n=73 Tax=Bacteria RepID=QUEC_BURCJ
          Length = 244

 Score =  189 bits (480), Expect = 7e-47,   Method: Composition-based stats.
 Identities = 102/228 (44%), Positives = 144/228 (63%), Gaps = 22/228 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGAR------- 56
           A+V+FSGGQDS TC+  AL++Y  V  + FDYGQRHR E++    +   L  R       
Sbjct: 10  ALVLFSGGQDSATCVAWALERYQTVETLGFDYGQRHRVELECREGVREALKHRFPAWSDR 69

Query: 57  --AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
                ++D+++L  ++ +++TR      + E  A+G+PNTFVPGRN++F+T+AA  AY+ 
Sbjct: 70  LGDDHMIDLSVLGAISDTAMTRT----IEIETTANGLPNTFVPGRNLMFMTIAAAIAYRR 125

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADY 174
               ++ G+CETDFSGYPDCRD+ +KAL  A++LGM   +  ETPLMW+DKA+TW LA+ 
Sbjct: 126 GLRVLVGGMCETDFSGYPDCRDDTMKALQVALNLGMDTRMVLETPLMWLDKAQTWQLAEQ 185

Query: 175 YG---KLDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
            G    ++L+R ET TCY G +      G GCG C AC LR  G   Y
Sbjct: 186 LGGDALVELIRVETHTCYVGERAELHDWGFGCGECPACKLRKRGYEAY 233


>UniRef50_Q8TWG9 7-cyano-7-deazaguanine synthase n=1 Tax=Methanopyrus kandleri
           RepID=QUEC_METKA
          Length = 229

 Score =  187 bits (476), Expect = 2e-46,   Method: Composition-based stats.
 Identities = 58/233 (24%), Positives = 89/233 (38%), Gaps = 18/233 (7%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQR-HRAEIDVARELALKLGARAH 58
           M   VV+ SGG DS+     A+++    V C+  DYGQR    E   A  +A  +GA   
Sbjct: 1   MATTVVLCSGGLDSSVIAKWAVEELGGRVICLFVDYGQRNAPFERRAAERIAEAVGAEFE 60

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE- 117
            V    L      + +    +P    E   + +   ++P RN   L +AA     +  E 
Sbjct: 61  TVGTFWLRRLCPDNPMFAGRLP---REAGTEDLSANWLPARNWNLLGVAAALCDHLYLEG 117

Query: 118 -----AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 ++ G+   +   +PD   EF  A+  A+  G+    R  +PL  + K     L 
Sbjct: 118 EDDEFHIVWGINAEEAERFPDNTKEFADAVAEALKRGLPSRPRLHSPLAELYKPGIVRLG 177

Query: 173 DYYGKLDLVRNETLTCYNGFKGD----GCGHCAACNLRANGLNHYLADKPTVM 221
                L      +++CYN    D     CG C AC  R         + PT  
Sbjct: 178 SE---LGAPMELSVSCYNPIWEDDTPVHCGECEACYHRKRAFERAGIEDPTEY 227


>UniRef50_A8AAU7 ExsB protein n=1 Tax=Ignicoccus hospitalis KIN4/I
           RepID=A8AAU7_IGNH4
          Length = 260

 Score =  186 bits (472), Expect = 6e-46,   Method: Composition-based stats.
 Identities = 64/245 (26%), Positives = 94/245 (38%), Gaps = 28/245 (11%)

Query: 5   VVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           V + SGG DS T    A+ ++  D    +T  YGQRH  E+  A E+A   GA+   V  
Sbjct: 4   VGLVSGGMDSATAFADAINKFNPDVKVALTVVYGQRHARELKAAAEVAKFYGAQHLVVDL 63

Query: 63  VTLLNELAV-SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV------K 115
             L  ++   S+L          E     +P T+VP RN + L  A     ++       
Sbjct: 64  SDLFEKVKEVSALLGGRGVPEAEELVPGLVPPTYVPQRNAVLLASAGAILEKLMIERNDG 123

Query: 116 AEAVITGVCETDFSG----YPDCRDEFVKALNHAVSLGM-----------AKDIRFETPL 160
              +  G   TD++     YPD R EFV+A+  A++ G               I    P 
Sbjct: 124 LGVISVGFHGTDYAPGEPVYPDTRPEFVEAMERALNEGSSVVFNFKRAGREAKITIYAPF 183

Query: 161 MWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV 220
           + + K +    A    +L +    T TCY G     CG C AC  R           P  
Sbjct: 184 VRLKKKDVVKRA---LELGVPLELTWTCYRGG-DRPCGTCPACVSRLRAFMEAGVPDPLT 239

Query: 221 MAAMK 225
            +  +
Sbjct: 240 ESYER 244


>UniRef50_C3XFP7 Queuosine biosynthesis protein queC n=1 Tax=Helicobacter bilis ATCC
           43879 RepID=C3XFP7_9HELI
          Length = 213

 Score =  185 bits (469), Expect = 1e-45,   Method: Composition-based stats.
 Identities = 51/212 (24%), Positives = 85/212 (40%), Gaps = 6/212 (2%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKVLDV 63
           +V+FSGG DSTT L  + ++    H + F Y   H   EID A ++          +   
Sbjct: 3   LVLFSGGVDSTTALFWSKKE-CATHALIFKYPSLHNKREIDAAVQICKDENVSYTLLDMS 61

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
           ++      + L   S  +P      + +    VP RN +FL++AA  A  +  + ++   
Sbjct: 62  SVFVGFRSALLQNSSESIPQDSYNLESMAKLVVPFRNGIFLSVAAGLAESLGYKEIVLAN 121

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
              D   YPDC   F+ ++N ++  G    ++  +P    DK E   L      L +   
Sbjct: 122 HAGDHPLYPDCTQSFIDSMNESIMQGSGGAVKLCSPFCNWDKKEIVKLG---LSLGVDYT 178

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
           +T +CY G +   C  C  C            
Sbjct: 179 KTYSCYRGGE-KHCNTCPTCIESNESFLANGI 209


>UniRef50_B3G2Q8 Putative uncharacterized protein n=1 Tax=Pseudomonas aeruginosa
           RepID=B3G2Q8_PSEAE
          Length = 222

 Score =  182 bits (462), Expect = 8e-45,   Method: Composition-based stats.
 Identities = 68/214 (31%), Positives = 98/214 (45%), Gaps = 14/214 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           ++ VV+ SGG DST CL+ A  +  EV  +   Y Q H  E+D A     + G    K++
Sbjct: 16  RKIVVLHSGGLDSTVCLLLAHSRGHEVVSLGVSYNQHHHVELDYAAAQCARYGIER-KIV 74

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            V       V    R    +         + + F+PGRN +FL LA+  A  + A+ V T
Sbjct: 75  SVAWSKPARVIPKNRSMAEIKKG------VSSAFLPGRNGIFLMLASAEAAGIGADEVWT 128

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+   DFSGYPDCR EF+++    +S  + K  +   PL    K +   LA     L L 
Sbjct: 129 GINSVDFSGYPDCRPEFIESFRKMLSYAIPKGPKLSAPLQKKSKPQIAKLAHR---LGLQ 185

Query: 182 RNETLTCYNGFKGD----GCGHCAACNLRANGLN 211
           R++T +CY     +     CG C AC L      
Sbjct: 186 RHDTWSCYRPKIAEAGFVPCGACDACKLHEFAWQ 219


>UniRef50_Q2IUE0 7-cyano-7-deazaguanine synthase 2 n=1 Tax=Rhodopseudomonas
           palustris HaA2 RepID=QUEC2_RHOP2
          Length = 210

 Score =  181 bits (460), Expect = 1e-44,   Method: Composition-based stats.
 Identities = 70/217 (32%), Positives = 98/217 (45%), Gaps = 14/217 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K+ +V+ SGG DSTTCL+QA  +  +V  +  DYGQR   E+  A     K     H V+
Sbjct: 4   KKVLVLHSGGMDSTTCLLQAKAEGHDVASLGIDYGQRLLVEMMFAEGQCEKYSIPRH-VI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +V          L R        E  A  +   F+PGRNI+FL L   +A  + A+ +  
Sbjct: 63  NVNWQKAERQIPLNRS------VEEMAHSVSPAFLPGRNIVFLGLGHAHAAGIGADELQI 116

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+   DFSGYPDC  +F  +    +++G     +   PL+ + K E   LA     L L 
Sbjct: 117 GLNCVDFSGYPDCTTQFYDSYCTMLNIGNPGGPKLVAPLLKMSKPEIARLAS---TLGLQ 173

Query: 182 RNETLTCYNG----FKGDGCGHCAACNLRANGLNHYL 214
           RN+T +CY           CG C AC L         
Sbjct: 174 RNDTWSCYRPQIREGSIVACGECDACKLHEFAWQELK 210


>UniRef50_D1R584 Putative uncharacterized protein n=1 Tax=Parachlamydia
           acanthamoebae str. Hall's coccus RepID=D1R584_9CHLA
          Length = 231

 Score =  179 bits (455), Expect = 5e-44,   Method: Composition-based stats.
 Identities = 78/228 (34%), Positives = 122/228 (53%), Gaps = 9/228 (3%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+AVV+ SGG DS+ CL  A++ +  + V  ++F Y QRH +E++ A+++        H 
Sbjct: 5   KKAVVIHSGGMDSSICLALAIRTFSVENVLSLSFIYDQRHSSELEQAKKICQSWNV-DHI 63

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +L +  L E+  ++LT  S+P+     EA   PNT V GRN L   L AI+A+++ A  +
Sbjct: 64  ILSIPCLKEITTNALTDTSLPIQCGNGEA---PNTLVVGRNGLMAQLGAIHAHELGAHCI 120

Query: 120 ITGVC--ETDFSGYPDCRDEFVKALNHAVSLGMAKD-IRFETPLMWIDKAETWALADYYG 176
             G+   E + SGY DC   ++      + L +       ETPL+ + K +T  LA   G
Sbjct: 121 YMGIIGVEGNHSGYRDCSRAYMDLKQTLLRLDLDDHLFTIETPLVDMSKKKTLELAQEMG 180

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
            L+ + NET+TCY G +  GC  C AC LR  G+  + A+ P+     
Sbjct: 181 VLEFLLNETITCYEGLEKLGCQKCPACILRNEGIRQFSAENPSFKLPY 228


>UniRef50_Q0BQ87 7-cyano-7-deazaguanine synthase n=76 Tax=Bacteria RepID=QUEC_GRABC
          Length = 252

 Score =  179 bits (454), Expect = 7e-44,   Method: Composition-based stats.
 Identities = 98/233 (42%), Positives = 132/233 (56%), Gaps = 27/233 (11%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV---ARELALKLGAR---- 56
           A+V+FSGGQDSTTCL  AL+++  V  + F YGQRH  E++     R++   +  R    
Sbjct: 18  ALVLFSGGQDSTTCLAWALERFAHVETIGFTYGQRHSVEMECRGRVRQVLAHMSGRSGAD 77

Query: 57  -------AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
                   H +     L+ +  S+LT  S      E    G+P +FVPGRN++FLT AA 
Sbjct: 78  WGSRLGDDHVLDLGNALHHVGQSALTGRS----PIEIGQGGLPTSFVPGRNLIFLTYAAA 133

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
             ++     ++ G+CETD+SGYPDCRD+ +KA+  A++ GM      ETPLMW+ KAETW
Sbjct: 134 LGWRRGLRRMVGGMCETDYSGYPDCRDDTIKAMQLALNTGMEAHFVLETPLMWLTKAETW 193

Query: 170 ALADYYGKLDLVR---NETLTCYNGFK------GDGCGHCAACNLRANGLNHY 213
           ALAD  G   LV     ET TCY G +      G GCG C AC+LRA G   +
Sbjct: 194 ALADSLGGPALVALIEEETHTCYLGDRSQRHPWGYGCGECPACSLRAEGWREW 246


>UniRef50_Q6MKZ5 7-cyano-7-deazaguanine synthase n=1 Tax=Bdellovibrio bacteriovorus
           RepID=QUEC_BDEBA
          Length = 231

 Score =  178 bits (451), Expect = 2e-43,   Method: Composition-based stats.
 Identities = 57/225 (25%), Positives = 99/225 (44%), Gaps = 15/225 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDE-VHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           K+ VV+ S G DST    +A++ + E V  +TF+YGQR  + E++ +  +A  LG   HK
Sbjct: 5   KKVVVLLSAGLDSTVNAYEAIKHHHEIVLALTFNYGQRAAKKELEASANIAKHLGI-PHK 63

Query: 60  VLDVTLLNELAVSSL--------TRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           V+++    +   SSL        T  ++ + + +   +   + +VP RN +FL +AA YA
Sbjct: 64  VVELPWFKDFNKSSLLVEDQAVPTGSAVEIDNQQKSEETAKSVWVPNRNGIFLNIAAAYA 123

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             + A+AVI G    + + +PD   EF++    ++    +  +        + K +   L
Sbjct: 124 EALGADAVIPGFNAEEAATFPDNSREFLEQATKSLWYSTSNHVTVGCYTAHLKKPDIVRL 183

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
                 L +       CY       CG C +C            D
Sbjct: 184 GQG---LKVPWELIWPCYFSG-DKWCGQCESCLRSKRAFASANID 224


>UniRef50_A4J5C7 7-cyano-7-deazaguanine synthase n=4 Tax=Clostridia RepID=QUEC_DESRM
          Length = 228

 Score =  174 bits (440), Expect = 3e-42,   Method: Composition-based stats.
 Identities = 55/223 (24%), Positives = 95/223 (42%), Gaps = 12/223 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           +VV+ SGG DS   L  A  + +    +T +Y QR   +   A     +  A  H+V+++
Sbjct: 3   SVVLLSGGLDSAVNLAFARTEGNVKLALTINYRQRAAHKEIAAAAQLARYYAIPHRVIEL 62

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEA--------DGIPNTFVPGRNILFLTLAAIYAYQVK 115
             L E+  ++L R    +P+ + E               +VP RN LF+ +AA +A  + 
Sbjct: 63  PWLAEITKTALVRSESQLPEPKTEELDQLELAGKTAAEVWVPNRNGLFVNIAACFAETLH 122

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
            E V+ G    + + +PD   EF  A + A+    A  ++  +    ++K +  AL    
Sbjct: 123 CEIVVAGFNCEEAATFPDNTPEFALAASAAMQYSTANQVKVLSYTGRLNKKDIVALGQR- 181

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKP 218
             L L      +CY G +   CG C +C       +    + P
Sbjct: 182 --LQLPWELIWSCYRG-EALMCGKCESCQRMIRAFHSLGLNLP 221


>UniRef50_UPI00016988F1 exsB protein n=1 Tax=Endoriftia persephone 'Hot96_1+Hot96_2'
           RepID=UPI00016988F1
          Length = 213

 Score =  167 bits (423), Expect = 2e-40,   Method: Composition-based stats.
 Identities = 71/163 (43%), Positives = 100/163 (61%), Gaps = 8/163 (4%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           KRAVV+ SGG DS T L  A QQ  E H ++  YGQRH AE+D AR++A  LGA  HK++
Sbjct: 5   KRAVVLLSGGLDSATVLAMARQQGFECHALSMLYGQRHAAELDAARQVAQALGAVDHKLI 64

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++  L+++  S+LT + I VP     ++GIP T+VP RN +FL+LA  +A  ++A+ +  
Sbjct: 65  NIG-LDDIGGSALTDEQIAVPKT--PSEGIPPTYVPARNTVFLSLALGWAEVLEAQDIFI 121

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM-----AKDIRFETP 159
           GV   D+SGYPDCR EF+ A     +L        + I   TP
Sbjct: 122 GVNAVDYSGYPDCRPEFIVAFEQLANLATKAGVEGRRIHIHTP 164


>UniRef50_B8GP80 ExsB family protein n=1 Tax=Thioalkalivibrio sp. HL-EbGR7
           RepID=B8GP80_THISH
          Length = 213

 Score =  164 bits (415), Expect = 2e-39,   Method: Composition-based stats.
 Identities = 53/221 (23%), Positives = 82/221 (37%), Gaps = 22/221 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLD 62
           ++++ SGG +S T L    +Q   V  +  DYGQR    E D AR      G    ++  
Sbjct: 12  SLLLLSGGIESATLLALESRQ-RPVTPLVVDYGQRGALRERDAARAQCESHGLTPVELDL 70

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                      L R               P+  +P RN++ L+LA  YA +   + V+  
Sbjct: 71  SATGGTFRDGLLKR---------------PHVPLPHRNLVILSLAVSYAARGGIDRVVLA 115

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           +   D + YP     F++    A+   +   +   TPL+  DKA    L      L +  
Sbjct: 116 LNRDDLAAYPSASPGFLEHF-RALIHDLQPGLEITTPLIDRDKAGVIRLGVS---LGVDF 171

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
             T +C  G++   CG C  C  R           PT    
Sbjct: 172 ARTWSCLLGYE-THCGACPQCLHRRAAFRAAGIADPTRYRH 211


>UniRef50_D2R400 ExsB family protein n=1 Tax=Pirellula staleyi DSM 6068
           RepID=D2R400_9PLAN
          Length = 236

 Score =  160 bits (406), Expect = 3e-38,   Method: Composition-based stats.
 Identities = 53/228 (23%), Positives = 84/228 (36%), Gaps = 17/228 (7%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKL---GARAHK 59
             V+ SGG DS+  + + L++   VH +    G      E+   +     +     R  K
Sbjct: 10  IAVLMSGGLDSSILVGKFLREGRTVHPIYIRTGLFWQSGELPAVQAFLAAIATANLRPLK 69

Query: 60  VLDVTLLNELAVS-SLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +L++ L +      SLT    P  D   EA      ++PGRN L L  AA+Y        
Sbjct: 70  ILELPLDDLYTDHWSLTGSGTPADDTPDEA-----VYLPGRNALLLVKAAVYCQLHGIGQ 124

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           +      +  S + D   EF      A++ G +  +    P   + K E   L      +
Sbjct: 125 LACAPLGS--SPFEDASSEFFTEYQSAMNRGSSSPVVLLRPFGELHKEEVMRLG-----V 177

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQ 226
            L    T +C +   G  CG C  C  R         +  TV  A ++
Sbjct: 178 GLPLELTFSCIHPVDGTHCGTCNKCFERKEAFRITGIEDKTVYTASRR 225


>UniRef50_B9ZKA9 ExsB family protein n=1 Tax=Thioalkalivibrio sp. K90mix
           RepID=B9ZKA9_9GAMM
          Length = 217

 Score =  159 bits (403), Expect = 6e-38,   Method: Composition-based stats.
 Identities = 47/220 (21%), Positives = 76/220 (34%), Gaps = 21/220 (9%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLDV 63
           +++ SGG +S+  L Q   Q   +  +  DYGQR    E    R L    G    + +D+
Sbjct: 15  LLLLSGGIESSALLAQRHAQGRPLRALWVDYGQRNAERERAAIRALGADYGVDPVE-MDL 73

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             L +                   ++ + +  +P RN+L L LA   A    A  ++  +
Sbjct: 74  RALRQAFAK--------------RSEWVAHVPLPQRNLLVLALAINLAEHQGAGRILLAL 119

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
              D    P  R  F+  L       +   +  E PL+   KA+          L +  +
Sbjct: 120 NREDQGHGPGSRPSFIGRLAALADE-LVPGLAIEAPLIAQSKADII---YTTDPLGIDWS 175

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
            T +C    +   CG C  C  R           PT    
Sbjct: 176 RTWSCLL-AQSLHCGRCPQCQARRAAFASAGVPDPTDYRE 214


>UniRef50_Q96ZD9 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Sulfolobus tokodaii
           RepID=QUEC1_SULTO
          Length = 202

 Score =  158 bits (400), Expect = 1e-37,   Method: Composition-based stats.
 Identities = 47/220 (21%), Positives = 89/220 (40%), Gaps = 24/220 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS++      ++  +  C+  +YGQR  R ++  ++ +  KL  +   V 
Sbjct: 2   KALLLMSGGLDSSSAAYYYTRRGLDFDCLFINYGQRSARMQLRSSKIICEKLN-KKLLVA 60

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+  + E     L    I +  +EP         +  RN++ + +A  +A +   E +I 
Sbjct: 61  DIRKIRE-----LFISDIWLKPHEP---------ITHRNLVIIPIAIAFAKEKGYEEIII 106

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              + D     +     ++ +    +LG    ++  TP   + K+    L    G   L 
Sbjct: 107 ASVKEDCEYEQNR----IEIIKELKNLGEILKVKVSTPFAGMPKSFLLKLGVSAG---LD 159

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            + T +C  G K   CG C+ C  R           PT  
Sbjct: 160 PSLTYSCLLGHK-YHCGQCSQCLKRKEAFKSANIQDPTKY 198


>UniRef50_B7GTM4 ExsB family protein n=2 Tax=Actinobacteridae RepID=B7GTM4_BIFLI
          Length = 251

 Score =  156 bits (395), Expect = 4e-37,   Method: Composition-based stats.
 Identities = 47/227 (20%), Positives = 74/227 (32%), Gaps = 11/227 (4%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHKVL 61
           R  ++ SGG DSTT L   L Q  E++ VTF++G+     E   A+ +   L +      
Sbjct: 7   RVALLLSGGPDSTTLLYDLLNQGKEIYAVTFNFGETEGCNESQHAQRIVTNLHSERLHHY 66

Query: 62  DVTL---LNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
                            +           A        P  + + L L A +A +     
Sbjct: 67  QYDFSEQFKRFYELPQPQFLRKAFGPFVVAPNKEEHVQPFGSAIALMLMASWALKHDIVD 126

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVS--LGMAKDIRFETPLMWIDKAETWALADYYG 176
           V   V   D + + D    + +AL+       G    + F TP + I+K           
Sbjct: 127 VYYAVHSND-AVFHDNHASYFEALHTVTVECEGDEYGVTFHTPYLSINKCAVIQKGMG-- 183

Query: 177 KLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
            L +   +T +C  G     CG C  C  R           P +   
Sbjct: 184 -LGVPFGDTWSCALGGTV-HCGVCDPCRDRRTSFEILGIKDPVLYEH 228


>UniRef50_A8URJ3 Putative uncharacterized protein n=1 Tax=Hydrogenivirga sp.
           128-5-R1-1 RepID=A8URJ3_9AQUI
          Length = 203

 Score =  156 bits (395), Expect = 5e-37,   Method: Composition-based stats.
 Identities = 49/225 (21%), Positives = 78/225 (34%), Gaps = 23/225 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG-QRHRAEIDVARELALKLGARAHK 59
           MK+  V+FS G +ST+ LV  L++      +    G    R E+  +  L   L  R   
Sbjct: 1   MKKVGVLFSAGVESTSLLVYYLERGYLTLPLYARCGLSWERTELKWSERLWAFLKYRYKN 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           ++ +       +                     +  +P RNI  +  A ++AY      V
Sbjct: 61  LMPLKTFPASGLQP----------------SKGSIEIPLRNITLVMGAVVHAYVKGIRKV 104

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G        +PD   +++  L   +S G  ++I  ETP M ++K E            
Sbjct: 105 ALG--SLGIYPFPDNNRDYLDRLEALISEGARENISLETPFMGMEKWEVVREFYR----K 158

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
                T +C     G  CG C  C  R  G        PT   + 
Sbjct: 159 APYRLTFSCVKPIIGKHCGKCEKCLERKEGFLKAGVPDPTSYVSS 203


>UniRef50_Q8ZVX7 7-cyano-7-deazaguanine synthase n=7 Tax=Thermoprotei
           RepID=QUEC_PYRAE
          Length = 269

 Score =  155 bits (392), Expect = 1e-36,   Method: Composition-based stats.
 Identities = 64/246 (26%), Positives = 98/246 (39%), Gaps = 34/246 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLG-------- 54
           R V V SGG DST      L++  +VH ++F YGQ+   E++ A+ L  +L         
Sbjct: 8   RVVAVVSGGPDSTCYTALWLKRGCDVHALSFLYGQKAVVEVERAQLLLRRLDGIAAERGW 67

Query: 55  --ARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
                H+V+D++ L EL   +   D    P    E    P   VP RN++   +A  YAY
Sbjct: 68  GRVVEHRVVDLSSLGELWRGTQLTD----PSVSVEQSYTPTVVVPIRNVVMAAVATAYAY 123

Query: 113 QVKAEA-----VITGVCETDFSG--------YPDCRDEFVKALNHAVSLGM---AKDIRF 156
            V   A     VI G    D           YPDC  E V+A+  A  +      + +  
Sbjct: 124 TVGRNAGAKTYVILGSHYDDIKPREDTWEPLYPDCSPECVEAMQTAFRICHFRGERGVEI 183

Query: 157 ETPLM-WIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
            TP    + K++   +     ++  +  +T +CY   +   CG C +C  R         
Sbjct: 184 WTPSREGLRKSQLLKMCHQ--EVGDLIYDTWSCYKSGEA-HCGSCESCKNRHAAFIEAGL 240

Query: 216 DKPTVM 221
              T  
Sbjct: 241 PDCTAY 246


>UniRef50_B9M1I5 ExsB family protein n=4 Tax=Proteobacteria RepID=B9M1I5_GEOSF
          Length = 196

 Score =  154 bits (388), Expect = 3e-36,   Method: Composition-based stats.
 Identities = 65/203 (32%), Positives = 93/203 (45%), Gaps = 16/203 (7%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVLD 62
            V + SGG DST     A ++    H +  DYGQR R  E+   R    +LG    +V D
Sbjct: 3   IVTLVSGGLDSTLIAKLAQEEGLIQHPLFVDYGQRARDQELAACRRGMSELGLPEPRVAD 62

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           ++    L  S LT  S+ +         + + F PGRN+LFL +AA YA QVKA+A+  G
Sbjct: 63  LSGFGALIHSGLTDSSLDI---------VKDAFTPGRNMLFLLIAAAYAVQVKADAISIG 113

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           +     S +PD    F++     +   M +DI+   PL    K +  ALA          
Sbjct: 114 LLHESTSLFPDQTSAFLRDAEVLIRRCMNRDIKLLAPLASFLKTDVVALAKLKR-----I 168

Query: 183 NETLTCYNGFKGDGCGHCAACNL 205
             T +C+ G +   CG C AC  
Sbjct: 169 GATYSCHTGNEET-CGTCIACRE 190


>UniRef50_A9U3Q1 Predicted protein n=1 Tax=Physcomitrella patens subsp. patens
           RepID=A9U3Q1_PHYPA
          Length = 290

 Score =  153 bits (386), Expect = 5e-36,   Method: Composition-based stats.
 Identities = 50/225 (22%), Positives = 82/225 (36%), Gaps = 21/225 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLD 62
           +VV+ SGG +S   L         +H +  +YGQR    E   + ++   LG    ++  
Sbjct: 6   SVVLMSGGIESAVLLYNWRSSSALLHPLYINYGQRGAAMEERASDKICNNLGLELSRIDM 65

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
             + N+                E +        +P RN++ L LAA YA QV +  V  G
Sbjct: 66  SAVGNKFR--------------ELQHGAKSFVPLPHRNLVILGLAASYASQVGSSKVALG 111

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
           + + D   Y      F+ +   + +  +   +    PL    KAE   L      L +  
Sbjct: 112 LNKDDLGSYSSASQNFMDSF-RSTAASLEPPVTLIAPLSHFTKAEVIRLGKR---LGVPF 167

Query: 183 NETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTV-MAAMKQ 226
            +T +C  G +   CG C  C  R          +P       +Q
Sbjct: 168 ADTYSCMVG-RPVHCGRCHQCRSRQLAFASASEQEPAGFYERYRQ 211


>UniRef50_B1XTB6 ExsB protein n=1 Tax=Polynucleobacter necessarius subsp.
           necessarius STIR1 RepID=B1XTB6_POLNS
          Length = 208

 Score =  150 bits (378), Expect = 4e-35,   Method: Composition-based stats.
 Identities = 53/184 (28%), Positives = 87/184 (47%), Gaps = 13/184 (7%)

Query: 12  QDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTLLNELAV 71
            DSTT L  A       + ++  YGQRH +E+  A+ +A ++G   H+V+++  L     
Sbjct: 22  IDSTTVLALAKDSDYTPYALSVGYGQRHSSELAAAKHIAKQIGVARHEVVNLD-LTRFGG 80

Query: 72  SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVCETDFSGY 131
           S+LT   I VP    +   IP T+VP RN + L+LA  +   +    +  G    D+SGY
Sbjct: 81  SALTDTKIAVPTSPGKDHEIPVTYVPARNTILLSLALGWTESLGGLDIFYGANSVDYSGY 140

Query: 132 PDCRDEFVKALNHAVSLGMAKDI---------RFETPLMWIDKAETWALADYYGKLDLVR 182
           PDCR E V +  H  +L     +         R   P++ + KA+   L      + +  
Sbjct: 141 PDCRPECVASFEHMANLATKAGVEAINNKNRFRVHAPIVSLTKAQIIQLGS---TMGVDY 197

Query: 183 NETL 186
           ++T+
Sbjct: 198 SQTV 201


>UniRef50_D2PHM6 ExsB family protein n=3 Tax=Sulfolobus islandicus
           RepID=D2PHM6_SULIS
          Length = 213

 Score =  149 bits (375), Expect = 8e-35,   Method: Composition-based stats.
 Identities = 49/223 (21%), Positives = 82/223 (36%), Gaps = 24/223 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHK 59
           + + +++FS G DS++      ++  +  C+  +YGQ   R ++   + +  KL  R   
Sbjct: 14  IMKFLLLFSSGLDSSSVAYYYAKKSLDFDCLFINYGQSSVRMQLKYGKIICEKLKRRLII 73

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +    L      +   R               P+  +  RN++ + +A  YA Q   E V
Sbjct: 74  INAKNLGEAFVNNDWLR---------------PHEPIIHRNLVIVPIAIAYAKQNGYERV 118

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           I      D    P+ R      +     LG    +RFETP +   K     +    G   
Sbjct: 119 IMATIREDCEYEPNRR----DVMEKLKELGEILGVRFETPFVNFPKYLLLKIGIKSG--- 171

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
           L  + T +C  G K   CG C+ C  R           PT+  
Sbjct: 172 LDPSLTYSCLLGHK-YHCGKCSQCLKRIEAFKQAKIRDPTIYK 213


>UniRef50_Q24ZT5 7-cyano-7-deazaguanine synthase 1 n=1 Tax=Desulfitobacterium
           hafniense Y51 RepID=QUEC1_DESHY
          Length = 220

 Score =  148 bits (373), Expect = 2e-34,   Method: Composition-based stats.
 Identities = 44/211 (20%), Positives = 87/211 (41%), Gaps = 12/211 (5%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGAR-AHKV 60
           + V++FSGG DST  L   L +  E+  +  +YGQ+ +  E++   ++   L  +     
Sbjct: 5   KVVLLFSGGIDSTVLLFWLLSRNYEIFPLFINYGQKSYEGELEAINKILKDLNTKNNLLT 64

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           L++  L  +   +L  +     +     +   + F P RN++ L++AA Y Y+++   + 
Sbjct: 65  LNMPELQLVGSGALVGEY--PKNISSHNEWYASEFFPNRNMILLSIAATYGYKLQISKIA 122

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            GV    +    D    F++A+   ++  +A       P     + +    A     L +
Sbjct: 123 IGVVGDSYQ---DTTRTFLEAMEMTLAQSIA-RYELIAPFAGHPRQKVIEEAYR---LQV 175

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLN 211
               T +C N      C  C +C  R   + 
Sbjct: 176 PLKSTFSC-NAMGNRHCLLCTSCYEREKAIQ 205


>UniRef50_C8PJP9 Queuosine biosynthesis protein QueC n=1 Tax=Campylobacter gracilis
           RM3268 RepID=C8PJP9_9PROT
          Length = 391

 Score =  147 bits (372), Expect = 2e-34,   Method: Composition-based stats.
 Identities = 55/179 (30%), Positives = 86/179 (48%), Gaps = 8/179 (4%)

Query: 48  ELALKLGARAHKVLDVTLLNELAV--SSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLT 105
           E++ + GA+  +   ++         S    D I     E     +P+T+VP RN +FL+
Sbjct: 214 EISSQSGAQPQQGDKISSKRAEGEIPSQSQSDKISPSSGESAFSDLPSTYVPFRNGVFLS 273

Query: 106 LAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWI 163
           +AA  A + + +A+  GV + D SGYPDC   F+ A   A+ LG ++D   R +TPL+ +
Sbjct: 274 IAAALAEKERCDAIFIGVVQEDSSGYPDCGASFIAAFERALELGTSRDFHPRIKTPLVHL 333

Query: 164 DKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
            KA+  +LA   G   +    T +CY   +   CG C +C LR  G     A      A
Sbjct: 334 SKAQIVSLALEIG---VPLELTWSCYE-REDAACGLCDSCLLRLKGFAGAGAKDKIPYA 388



 Score = 88.6 bits (218), Expect = 1e-16,   Method: Composition-based stats.
 Identities = 30/93 (32%), Positives = 46/93 (49%), Gaps = 1/93 (1%)

Query: 3  RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKVL 61
          RA+ V SGG DS TC   A ++  E+  + FDY QR    E +   ++   LG     +L
Sbjct: 2  RALCVISGGMDSATCAYIARREGYEIVALHFDYDQRTMGKERECFGKICDDLGVAKRLIL 61

Query: 62 DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT 94
          D   + ++  S+LT  ++P+     E  G  NT
Sbjct: 62 DARFIAQIGGSALTDGTLPIRKGAAELCGAANT 94


>UniRef50_Q13AQ3 ExsB n=6 Tax=Proteobacteria RepID=Q13AQ3_RHOPS
          Length = 206

 Score =  147 bits (370), Expect = 4e-34,   Method: Composition-based stats.
 Identities = 49/212 (23%), Positives = 89/212 (41%), Gaps = 12/212 (5%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLG-ARAHKVL 61
           A+++ SGG DSTT     +++      + FDYGQ     E +  +E+    G       +
Sbjct: 3   ALLLCSGGLDSTTVGYWLVEKNISFQPLFFDYGQHCVEKEWETLQEVLPISGLVSPLLRI 62

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +++ + + + S L    I  P+   E     + ++P R ++F ++ A  A     +AV  
Sbjct: 63  NISEIFQHSPSRL----IAEPNLWTEHVEDDDLYIPYRTLMFFSVGACIAQTRGLDAVYA 118

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G   ++ +   DC   F+  L+          +RFE P     KA+    A+    L + 
Sbjct: 119 GFINSNHAKELDCSAAFLNNLDSLSR--SLGPVRFELPFRDWSKADVVRRANE---LRVP 173

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHY 213
             +T +C   F    CG C  C  R + +  +
Sbjct: 174 VGKTYSC-QLFSDTPCGACPNCVERISAIESF 204


>UniRef50_A4YH21 ExsB family protein n=1 Tax=Metallosphaera sedula DSM 5348
           RepID=A4YH21_METS5
          Length = 199

 Score =  146 bits (368), Expect = 6e-34,   Method: Composition-based stats.
 Identities = 51/220 (23%), Positives = 80/220 (36%), Gaps = 26/220 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-IDVARELALKLGARAHKVL 61
           +++ + SGG DST  + +  +   +  C+T DYGQR   E +  AR    KLG R  +V 
Sbjct: 2   KSLFLVSGGLDSTAGMYRFREMDYD--CMTIDYGQRAFKEQVKYARINCEKLGKRLIEVN 59

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
              + +                       IP+  +  RN + +  A  YA ++   AV  
Sbjct: 60  MKKVGDRFREG---------------KSLIPHEPIRHRNAVTIPFALTYASEMGYSAVYV 104

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
                +   Y   + E + AL        A  I    P + + KA    +   +G   L 
Sbjct: 105 FTVSEEC-QYESNKPEIISALRKL---AEALRIGLIFPFLGLSKAHVLRMGIEHGMDPL- 159

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
              T +C  G +   CG C+ C  R         D  T  
Sbjct: 160 --NTYSCLLGHRA-HCGRCSQCESRKMAFKIAGVDDKTKY 196


>UniRef50_UPI00016C5260 ExsB n=1 Tax=Gemmata obscuriglobus UQM 2246 RepID=UPI00016C5260
          Length = 232

 Score =  142 bits (357), Expect = 1e-32,   Method: Composition-based stats.
 Identities = 49/223 (21%), Positives = 71/223 (31%), Gaps = 15/223 (6%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKL---GARAHKV 60
            V+ SGG DS   L +A++    V  +    G      E          +     R  +V
Sbjct: 20  AVLVSGGLDSAILLAEAVRACPVVFPLYVRSGLHWEAVERAYLERFLAAIAAPNLRPLRV 79

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           LD    +       T     VP  +   +     F+PGRN+L L    I+ +      V 
Sbjct: 80  LDQPTADVYGAHWSTTGEG-VPGADSADEA---VFLPGRNVLLLAKPLIWCHLNAVPFVA 135

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           T    T+   +PD    F       VS  +  D+R   P   + K +             
Sbjct: 136 TAPLGTN--PFPDATPAFYDGFAGIVSSAVRGDVRVLRPYAALHKVDVMRRGR-----GR 188

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
               T +C    +G  CG C  C  R           PT  A 
Sbjct: 189 PLEHTFSCIRPVRGLHCGTCNKCAERRQAFRDAHMTDPTEYAE 231


>UniRef50_D2L9Z8 ExsB family protein n=1 Tax=Desulfovibrio sp. FW1012B
           RepID=D2L9Z8_9DELT
          Length = 201

 Score =  140 bits (353), Expect = 3e-32,   Method: Composition-based stats.
 Identities = 51/213 (23%), Positives = 86/213 (40%), Gaps = 21/213 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-EIDVARELALKLGARAHK 59
           +K + ++ SGG DST+        +      T DYGQ+    EI  AR++A ++G     
Sbjct: 2   VKNSAILLSGGMDSTSLAFWKKPNF----AFTIDYGQKAAMAEIRAARQVAKEIGISHDT 57

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +        +  SSL    + +    P A      + P RN L +TLAA+ A       +
Sbjct: 58  I-------AIDCSSLGSGDMAMAAPLPNAPSTD--WWPFRNQLLITLAAMKAICRNINLL 108

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G+ ++D S Y D   +FV  ++  +S     +I    P + +  AE   ++     L 
Sbjct: 109 YIGLVKSD-SSYKDGCSDFVDKISRTLSF-QEGNIEVMAPAIDMTAAELVNMSKIPRTL- 165

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNH 212
                + +C+       CG C  CN     +  
Sbjct: 166 --LAWSHSCH--VSDFACGRCRGCNKHRLVMEE 194


>UniRef50_A8V022 Trans-regulatory protein ExsB n=1 Tax=Hydrogenivirga sp. 128-5-R1-1
           RepID=A8V022_9AQUI
          Length = 166

 Score =  139 bits (351), Expect = 5e-32,   Method: Composition-based stats.
 Identities = 47/170 (27%), Positives = 73/170 (42%), Gaps = 12/170 (7%)

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           D+  L  +  S+LT + + +P  +   D  P T VP RN+ FL++AA +    + E +  
Sbjct: 3   DIPHLKGIEGSALTDEKVDIPSEDY-PDQPPITTVPMRNLNFLSIAASFCDVYEIENIGI 61

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGM------AKDIRFETPLMWIDKAETWALADYY 175
           G+   D S YPDCR EF  +    ++            I+  TP + + K +   L    
Sbjct: 62  GIHSVD-SPYPDCRAEFASSAEAVINASSVMVAKKKNRIKVYTPFLGMTKTDVLKLG--- 117

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMK 225
            +L +   +T +CY G     CG CA C  R           P V A  +
Sbjct: 118 LELGVPYEKTYSCYRGTIP-PCGECATCRQRQEAFESVGVKDPLVKADER 166


>UniRef50_Q12C85 ExsB n=1 Tax=Polaromonas sp. JS666 RepID=Q12C85_POLSJ
          Length = 282

 Score =  139 bits (349), Expect = 9e-32,   Method: Composition-based stats.
 Identities = 52/232 (22%), Positives = 86/232 (37%), Gaps = 9/232 (3%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHK 59
           + +A+VVFSGG DS    + A  +  +   +TF + QR +  E+  +  LA  LG     
Sbjct: 30  VTKAIVVFSGGPDSLAAALWAKSKKYDPWLLTFQFKQREQGGEMFASMLLAKTLGFPQEI 89

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +   + ++       T   +        AD   +  +     + +  A  +A   + + V
Sbjct: 90  IDFKSPMHSFEP---TVHVLMHAGTTTTADRAQHHRMEFGAGMVMATACTFAVYHQIDDV 146

Query: 120 ITGVCETDFSGYP-DCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           + G  + D  G   +   EF  A  + VS    +  R   P     K +   LA++ GK 
Sbjct: 147 VWGATKDDVFGGRFEYSQEFADAFANLVSCTTGRPFRIHVPFASKSKHQV--LAEFSGKE 204

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQKTGL 230
           DL    + +C        CG C AC  R            T       K+ L
Sbjct: 205 DL-FAMSWSC-KVGSSIQCGTCTACVARRVAAQASGVKDATSYHEQNYKSPL 254


>UniRef50_Q989H2 Mll6423 protein n=2 Tax=Proteobacteria RepID=Q989H2_RHILO
          Length = 198

 Score =  137 bits (346), Expect = 2e-31,   Method: Composition-based stats.
 Identities = 54/217 (24%), Positives = 84/217 (38%), Gaps = 22/217 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           M  A+ + SGG DS +              +T DYGQ+   AEID A  +A  LG   H+
Sbjct: 1   MTTAL-LLSGGMDSVSIAWWLRPD----VALTIDYGQKPAEAEIDAAGHVAQLLGIS-HE 54

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V+ V   +          S  +    P+A    + + P RN L +TLA + A +     +
Sbjct: 55  VIRVDCSSL--------GSGDMAGTAPDAAAPVSEWWPFRNQLLVTLAGMAAVRHGVSRL 106

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           + G   TD + + D R EFV+  +  +++     I  E P + +   +   L    G   
Sbjct: 107 LIGALATDGA-HADGRPEFVERASQLMAM-QEGGIVVEAPAIEL---DAVELVRRSGIDQ 161

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
                  +C+ G     CG C  C    N        
Sbjct: 162 GTLAWAHSCHTGN--YACGRCRGCTKHFNTWQALGWT 196


>UniRef50_C7RWC1 ExsB family protein n=4 Tax=Proteobacteria RepID=C7RWC1_9PROT
          Length = 201

 Score =  132 bits (331), Expect = 1e-29,   Method: Composition-based stats.
 Identities = 47/215 (21%), Positives = 79/215 (36%), Gaps = 21/215 (9%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKLGARAHKVL 61
            A ++ SGG DS +              +T DYGQ    AEI  ++ +  +L    H VL
Sbjct: 2   TAALLLSGGMDSISIAWWKHPD----IAITIDYGQLPAAAEITASQAICRRLRI-PHHVL 56

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            V   +          S  +   EP A      + P RN L +TLAA+ A  ++   +  
Sbjct: 57  QVDCHSL--------GSGDMAGLEPNALAPATDWWPYRNQLLITLAAMKAVTLEVRQLWL 108

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G   +D S + D    F+ A++  +++     +    P + +   +        G    +
Sbjct: 109 GTVASDAS-HRDGTPNFLSAMSALLAV-QEGGLTVSAPAIGMSTVDLVR---QSGVPLEI 163

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
                +C+       CG C  CN     L     +
Sbjct: 164 LAWAHSCHKAD--VACGACRGCNKYFEVLRELGNE 196


>UniRef50_C9Q119 Putative uncharacterized protein n=1 Tax=Prevotella sp. oral taxon
           472 str. F0295 RepID=C9Q119_9BACT
          Length = 432

 Score =  131 bits (329), Expect = 2e-29,   Method: Composition-based stats.
 Identities = 44/248 (17%), Positives = 76/248 (30%), Gaps = 41/248 (16%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
              + SGG DS    +  L+   E   V+  YG                 G +  KV   
Sbjct: 154 VFCMLSGGLDSFIGAINLLKDGKEPIFVS-HYG-----------------GGKGVKVYQD 195

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT-- 121
           +++N L +            Y    +G  +T    R+++F   A + A  +     +   
Sbjct: 196 SVVNSLKIQYAVDSKRFFRFYAASQNGAEDTT-RSRSLMFFAHAIVLASGIGHHVDLYVP 254

Query: 122 --GVCETDFS---------GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
             GV   +                   F+      +  G+  DI    P     K E   
Sbjct: 255 ENGVISLNIPLTVMRLGSLSTRTTHPYFMGMFQKLL-TGLDIDISLVNPFQMQTKGEMMK 313

Query: 171 LADYYGKLDLVRNETLTCYNGFKGD-------GCGHCAACNLRANGLNHYLADKPTVMA- 222
                  L+    +TL+C +  +G         CG C  C +R   +     +  T    
Sbjct: 314 TCKDLDFLNAHYQDTLSCSHPDQGRWTGDAVGHCGECLPCTIRKAAIKAAGLEDVTTYRH 373

Query: 223 AMKQKTGL 230
             + +TG+
Sbjct: 374 HYETETGI 381


>UniRef50_C3NEW9 ExsB family protein n=5 Tax=Sulfolobus RepID=C3NEW9_SULIY
          Length = 244

 Score =  130 bits (327), Expect = 3e-29,   Method: Composition-based stats.
 Identities = 36/222 (16%), Positives = 73/222 (32%), Gaps = 33/222 (14%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHK 59
           ++ A++  +GG DST        +   +H     YG   +  E+++ +EL+ KL      
Sbjct: 2   VEEAIIQLTGGIDSTVLAYYLKDK-YVLHGTFIYYGYPPQIRELELVKELSKKLDVPLKI 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +   + +              +P  +     I   FV       + + A ++       +
Sbjct: 61  IDFSSYIKSF----------DLPPIDSIQYIIKYQFV-------IEILASFSAYPNLNTM 103

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G  + +       +   +K +   +        +   P   + K+E   L +    L 
Sbjct: 104 FVGWLKDE----WSNKMSILKGIESLM------GFKVMAPFHTMVKSEVIKLGNE---LG 150

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           +   +T +C    K   CG C  C  R           PT+ 
Sbjct: 151 VDFTKTHSCIVSGK-MHCGICMPCRSRRRAFEEAKVKDPTIY 191


>UniRef50_B9LWH7 ExsB family protein n=1 Tax=Halorubrum lacusprofundi ATCC 49239
           RepID=B9LWH7_HALLT
          Length = 195

 Score =  128 bits (321), Expect = 2e-28,   Method: Composition-based stats.
 Identities = 51/211 (24%), Positives = 82/211 (38%), Gaps = 21/211 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKLGARAHK 59
           M  +V++ SGG DS T              VT DYGQ    AEI  + ++A +L    H+
Sbjct: 1   MPESVLLLSGGLDSATLAYTHRPD----LTVTVDYGQCCADAEIQASAQIADQLNLE-HE 55

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V++V   N  A +   +    + D           + P RN L +TL A+   +  A+ +
Sbjct: 56  VIEVDCENLGAGTMAGQQETDLGDA--------PEWWPYRNQLVVTLVAMKVVRRGADRL 107

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           + G    D S + D   EF + ++  +S     D+    P +     E           +
Sbjct: 108 LVGAVADDQS-HADGEAEFFELMDSLLSF-QEGDLGVVAPAIDRTTEEFV---CEVNPPE 162

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
            +   T +C        CG C +C  R   L
Sbjct: 163 SLLGWTHSC--TASNTACGECRSCKKRQRVL 191


>UniRef50_Q9RZH7 Putative uncharacterized protein n=1 Tax=Deinococcus radiodurans
           RepID=Q9RZH7_DEIRA
          Length = 423

 Score =  125 bits (315), Expect = 9e-28,   Method: Composition-based stats.
 Identities = 38/236 (16%), Positives = 64/236 (27%), Gaps = 34/236 (14%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           ++  V + SGG DS   ++  + +      V+    Q    +    RE+A  LG   H  
Sbjct: 136 IEDCVALLSGGLDSLVGMIDLVAEGHNPLAVS----QLVAGDAAKQREIATDLGRTTH-- 189

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                        L  +         E      T +     +    A     Q +A  + 
Sbjct: 190 -------------LQLNHAAECPTPGEDSQRSRTLIFLAYAVLAATATTRFQQARAVPIY 236

Query: 121 T---GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAET 168
               G    +    P              F++ L   +   +  ++    P     K E 
Sbjct: 237 INENGFIALNPPLTPMRIGSLSTRTAHPVFLQQLQAVL-QAVGLNVALINPYALKTKGEM 295

Query: 169 WALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
                    L    + T +C  Y  F    CG C  C +R      +     T   
Sbjct: 296 LTECANQTVLSRWASATTSCGRYRRFGYKHCGRCIPCQVRRAAFLAWGQPDRTEYV 351


>UniRef50_Q2RGB7 ExsB n=1 Tax=Moorella thermoacetica ATCC 39073 RepID=Q2RGB7_MOOTA
          Length = 192

 Score =  124 bits (311), Expect = 2e-27,   Method: Composition-based stats.
 Identities = 39/215 (18%), Positives = 65/215 (30%), Gaps = 31/215 (14%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHK 59
           M   +V+ SGG DS   +     +   V+ +   YGQ     E   A+ +A   G     
Sbjct: 1   MNPVLVLLSGGIDSAALVHYYRSKNTSVYGLHVQYGQPVAEREAQAAKAIAAHYGIDLRI 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
                 L       L                        RN +F+ +A           V
Sbjct: 61  AHIQPPLARKGEEYL-----------------------CRNAIFVLIACAN-LSPGINRV 96

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
             G+     + Y DC   F+  +   +    +  +  E P +   K E +     +G   
Sbjct: 97  ALGIHSG--TIYYDCSQGFMADMQRLLDGYFSGAVTVEAPFLRWSKLEVYQYCTSHG--- 151

Query: 180 LVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYL 214
           +  + T +C      + CG C +C  R        
Sbjct: 152 VPVDLTYSCER-NNDEPCGQCPSCYDRRRLYEAGG 185


>UniRef50_B1ZBL1 ExsB family protein n=4 Tax=Proteobacteria RepID=B1ZBL1_METPB
          Length = 214

 Score =  124 bits (311), Expect = 2e-27,   Method: Composition-based stats.
 Identities = 51/213 (23%), Positives = 73/213 (34%), Gaps = 22/213 (10%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVLDV 63
           V++FSGG DST              C+  DYGQR    E   A  +A + G R       
Sbjct: 3   VLLFSGGLDSTALAWWQRPD----ACLFVDYGQRPAAGERAAATSIAAEFGLR------- 51

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             L  L V        P+   EP A      + P RN + +TLA +         ++ G 
Sbjct: 52  --LETLQVDLSGLGLGPLAGGEPAALARAPEWWPYRNQILVTLAGMRFVAEGLSEILIGT 109

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
             TD   + D R  F++ L+  + L     +R   P   +      A     G       
Sbjct: 110 VATDV--HADGRAPFLRTLDRLMRL-QEGGVRVVAPARHLSGPALLA---ASGLPASALG 163

Query: 184 ETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
            T +C+       CG C  C      L  +   
Sbjct: 164 ATFSCH--VMEYACGQCRGCEKHRETLRSFGLR 194


>UniRef50_Q0BZ62 Putative uncharacterized protein n=1 Tax=Hyphomonas neptunium ATCC
           15444 RepID=Q0BZ62_HYPNA
          Length = 188

 Score =  121 bits (304), Expect = 2e-26,   Method: Composition-based stats.
 Identities = 49/203 (24%), Positives = 80/203 (39%), Gaps = 31/203 (15%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV-ARELALKLGARAHKVLDV 63
           VV+ SGG DS  C             V  DYG     E    A ++   LG    K    
Sbjct: 10  VVLVSGGIDSAACAAMYANNGGSTTGVFVDYGHPAAIEEFASAEKVCAALGIPLLK---- 65

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
                   +S + D I  P             + GRN L +  +A++A  +K   +  GV
Sbjct: 66  --------TSFSTDRITGPGE-----------IVGRNALLIW-SAVFASGIKKGVIAIGV 105

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
                + Y DC   F+  +++ ++      +R   P +   K+E ++   Y  +  +  +
Sbjct: 106 HAG--TTYYDCSSTFITRMDNMLAESTNGLVRISAPFVNASKSEVFS---YLQRSKVEAS 160

Query: 184 ETLTCYNGFKGDGCGHCAACNLR 206
            T +C +G K + CG CA+C  R
Sbjct: 161 LTYSCESGSK-EPCGICASCQDR 182


>UniRef50_Q3JDB4 Putative uncharacterized protein n=1 Tax=Nitrosococcus oceani ATCC
           19707 RepID=Q3JDB4_NITOC
          Length = 425

 Score =  120 bits (302), Expect = 3e-26,   Method: Composition-based stats.
 Identities = 36/240 (15%), Positives = 64/240 (26%), Gaps = 45/240 (18%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V + SGG DS    +  +        V+    Q    +     + A K+G   + +    
Sbjct: 141 VTLLSGGLDSFVGAIDLVADGKAPFAVS----QVAAGDKQSQADFAAKIGGGLNHLQL-- 194

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLT--LAAIYAYQVKAE----A 118
                             ++  +  G        R+ +FL   + A  A +   +     
Sbjct: 195 ------------------NHNVKCPGENERSQRARSFIFLAYGVLAASAQKHYHDGERVD 236

Query: 119 VIT---GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKA 166
           +     G+   +    P              F+      + +     I    P  +  K 
Sbjct: 237 LYICENGLISINPPLTPARLGSLSTRTTHPVFLGLFQRLLDVA-ELRIAVRNPYQFRTKG 295

Query: 167 ETWALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
           E          L     ET +C  Y       CG C  C +R    + +  D  T     
Sbjct: 296 EMLLECKDQAFLRKHAAETTSCGRYARNGWQHCGRCFPCLIRRAAFHAWGKDDRTKYVYA 355


>UniRef50_C6ALB1 Putative uncharacterized protein n=2 Tax=Proteobacteria
           RepID=C6ALB1_AGGAN
          Length = 438

 Score =  120 bits (300), Expect = 5e-26,   Method: Composition-based stats.
 Identities = 39/238 (16%), Positives = 73/238 (30%), Gaps = 36/238 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K  V +FSGG DS    +  L+Q      V+  Y                  G ++ +  
Sbjct: 147 KDCVCLFSGGLDSAIGTIDLLEQGHSPVLVSHSYK-----------------GDKSRQQA 189

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV------K 115
            +  LN+    +       +              +  R++ FL  A + AY +      K
Sbjct: 190 IIQQLNQNGYINQFSQFNAIAQPHLNNGKSTEITMRTRSLNFLAFAIVSAYALQKVAQKK 249

Query: 116 AEAVIT--GVCETDFS---------GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWID 164
            + ++   GV   +                   F++ +       +        P  +  
Sbjct: 250 IDILVPENGVISINAPLTVRRVGTLSTRTTHPYFIQEIQKLF-TAVNIPFTLRNPYQFKT 308

Query: 165 KAETWALADYYGKLDLVRNETLTC-YNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           K +  A       L  +  +T++C +   K   CG C  C +R   L++      T  
Sbjct: 309 KGQMIAECRNLPLLQQIIPDTVSCSHWKRKNQQCGVCVPCLIRRASLHYAGITNDTQY 366


>UniRef50_B8JF98 PP-loop superfamily ATPase-like protein n=1 Tax=Anaeromyxobacter
           dehalogenans 2CP-1 RepID=B8JF98_ANAD2
          Length = 447

 Score =  119 bits (298), Expect = 8e-26,   Method: Composition-based stats.
 Identities = 41/237 (17%), Positives = 68/237 (28%), Gaps = 39/237 (16%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V +FSGG DS        +   ++  V+      H  ++  + +       +      V 
Sbjct: 142 VGLFSGGLDSIVGTYLRSRTAKKLIVVSV-----HGIQVRASAQQRAITMLQRASFFSVP 196

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
           L        L     P                  R  LFL++ A  A  V A  + T   
Sbjct: 197 LHWVSFDHQLRHAHPPEKSQRC------------RGFLFLSIGAAVARTVGASTLHTYEN 244

Query: 125 ETDFSGYPDC------------RDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                  P                  + +L   + +     +R E P ++  K E    A
Sbjct: 245 GIGALNPPMNLAQVGAQNTRAMHPGTLASLEGILEVVQDDPVRLEAPFLFSTKGEMCRDA 304

Query: 173 DYYGKLDLVRNE-TLTCYNGFKGD-----GCGHCAACNLRANG-LNHYLADKPTVMA 222
             +    +   + + +C  G  G       CG C +C LR +          PT   
Sbjct: 305 REHL---IPLAQASNSCDEGEHGKPDPAEHCGLCTSCLLRRSALFAALGDKDPTPYR 358


>UniRef50_B0V938 Putative uncharacterized protein n=4 Tax=Acinetobacter baumannii
           RepID=B0V938_ACIBY
          Length = 442

 Score =  119 bits (297), Expect = 1e-25,   Method: Composition-based stats.
 Identities = 39/241 (16%), Positives = 71/241 (29%), Gaps = 43/241 (17%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           +V +FSGG DST   +    Q  +   V+  Y +  + +               H   D+
Sbjct: 150 SVCLFSGGLDSTIGAIDLKAQGKKSVLVSHAYPKDRQKQ--------------DHVYYDL 195

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA-------YQVKA 116
              N             + +     +   +  +  R+  F+   A+ A       +  K 
Sbjct: 196 QFDNARFQ--------VLANPRKVKEIPADVQMRTRSFNFIAFGALIATALSKNHFSNKI 247

Query: 117 EAVIT---GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWID 164
             +     G+   +    P              F++ LN      +   +  E P  +  
Sbjct: 248 IKLYIPENGLISINPPLTPRRIGALSTRTTHPYFLQKLNILFK-NIGLPVEIENPYQYKT 306

Query: 165 KAETWALADYYGKLDLVRNETLTCYNGFK-GDGCGHCAACNLRANGLNHYLADKPTVMAA 223
           K E          L  +   T++C    + G  CG C  C +R    + Y     T    
Sbjct: 307 KGEMMLECKDQSSLKKIAATTVSCGKWKRSGMQCGKCLPCLIRRASFDKYQYKDLTQYQY 366

Query: 224 M 224
            
Sbjct: 367 S 367


>UniRef50_A9GG06 Putative uncharacterized protein n=1 Tax=Sorangium cellulosum 'So
           ce 56' RepID=A9GG06_SORC5
          Length = 435

 Score =  117 bits (294), Expect = 2e-25,   Method: Composition-based stats.
 Identities = 41/247 (16%), Positives = 79/247 (31%), Gaps = 33/247 (13%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYGQRHRAEIDV--ARELALKLGARAHKV 60
           AV +FSGG DS    +  L+   +   V    Y   H        A EL  + G      
Sbjct: 117 AVCLFSGGVDSLIGAINLLESDRKSRVVLVGHYDSTHTPGTQKVLADELIQEYGDER--- 173

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK-AEAV 119
             + L   +  + +       P    +     +     R+++FL L    A  +  A  +
Sbjct: 174 -VLPLRVLVRPAPIRDGVQAYPLPAVKESTTRS-----RSLVFLGLGMAVAAALGPAVPL 227

Query: 120 IT---GVCETDFSGYPD---------CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
                G    +   +               F+  + +A+ + +        P ++  K E
Sbjct: 228 YIPENGFIAINVPLHGSRVGSCSTRTTHPYFLDRIANALGV-VGVKNPIVNPYVYKTKGE 286

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGDG-------CGHCAACNLRANGLNHYLADKPTV 220
                     L  V + T++C +  +          CG+C  C +R    N    +  ++
Sbjct: 287 MVRECKNQDLLRRVAHITVSCAHPERKQNETNTWPNCGYCYPCLIRRAAFNWAGFESSSM 346

Query: 221 MAAMKQK 227
            A  ++ 
Sbjct: 347 YARYRRD 353


>UniRef50_Q5WAJ2 Putative uncharacterized protein n=4 Tax=Bacillus
           RepID=Q5WAJ2_BACSK
          Length = 430

 Score =  116 bits (290), Expect = 7e-25,   Method: Composition-based stats.
 Identities = 35/243 (14%), Positives = 76/243 (31%), Gaps = 41/243 (16%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYGQRHRAEIDVARELALKLGARAHKVL 61
           + V +FSGG DS    ++ L    E   +    YG      I   +++  +     +++ 
Sbjct: 145 KKVCMFSGGLDSFIGAIELLDDTSENDTLFVSHYG--GGKGIKEYQDILREELISKYQLT 202

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK-AEAVI 120
           +    +  A                  +G+ +T    R+ +F + A + A  V     ++
Sbjct: 203 ESNFYSFYAS---------------AKNGVEDTT-RTRSFMFFSHAVVLASSVDRPVNLV 246

Query: 121 T---GVCETDF---------SGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAET 168
               G+   +          S        ++      +   +   ++   P  +  K E 
Sbjct: 247 IPENGLISLNIPLTNSRLGSSSTRTTHPFYLSLFQKLLD-NLKIMVKILNPYQFKTKGEM 305

Query: 169 WALADYYGKLDLVRNETLTCYNGFKGD--------GCGHCAACNLRANGLNHYLADKPTV 220
                    L      T++C +   G          CG+C  C +R + + +      T+
Sbjct: 306 IRECSSIEFLQKNITNTMSCSHPDWGRWAGESEVCHCGYCLPCVIRRSAIKNAGIQDETI 365

Query: 221 MAA 223
              
Sbjct: 366 YRD 368


>UniRef50_Q4UTY2 Putative uncharacterized protein n=2 Tax=Gammaproteobacteria
           RepID=Q4UTY2_XANC8
          Length = 428

 Score =  116 bits (290), Expect = 7e-25,   Method: Composition-based stats.
 Identities = 34/241 (14%), Positives = 63/241 (26%), Gaps = 45/241 (18%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + +V + SGG DS    +   +       V+                    +    H   
Sbjct: 141 EDSVCLLSGGMDSLIGAIDLAESGRRSIAVS------------------NTVPGDGHNQH 182

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA----- 116
                       L  ++ P  D   E           R+I+FL    + A  ++A     
Sbjct: 183 SFAEAVGFKHLPLNHNASPPSDGSREPSQ------RARSIIFLAFGVLAATSLQAYQDGA 236

Query: 117 -EAVIT---GVCETDFS---------GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI 163
              +     G    +                  EF+  +   +       +    P    
Sbjct: 237 QVPLFICENGFIALNPPLTGTRLGSLSTRTAHPEFLHGVQSVLDAA-GLRVNLINPYQHK 295

Query: 164 DKAETWALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
            K E          L  +   +++C  Y  +  + CG C  C +R +    +    PT  
Sbjct: 296 TKGEMSRECADQDLLRNLAASSVSCGRYRRYGYNQCGRCIPCQIRRSAYLAWGQPDPTDY 355

Query: 222 A 222
            
Sbjct: 356 V 356


>UniRef50_B1M991 Putative uncharacterized protein n=2 Tax=Proteobacteria
           RepID=B1M991_METRJ
          Length = 435

 Score =  116 bits (290), Expect = 7e-25,   Method: Composition-based stats.
 Identities = 38/229 (16%), Positives = 68/229 (29%), Gaps = 22/229 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVAREL---ALKLGARAHKV 60
           +  +FSGG DS    +  + Q +    V+  Y    R +   A  L     +  A AH  
Sbjct: 150 SACLFSGGLDSYIGALDLIAQGERPLLVSHAYRGDRRYQDRTAHALPVACARFSANAHPT 209

Query: 61  LDVTL---LNELAVSSLTRDSIPVPDYEPEADGIPNT-FVPGRNILFLTLAAIYAYQVKA 116
                   +   + + L   +           G   T +VP   ++ L   A    +   
Sbjct: 210 WSGPSDVSMRTRSFNFLAYGAAAATAISQMRAGNRVTLWVPENGLIALN--APLTPRR-- 265

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
                G   T           F++++ + +   +   +    PL    K E         
Sbjct: 266 ----IGSHST-----RTTHPYFLQSMQNILD-AVGLPVAIVNPLRHQTKGEMVLRHARDP 315

Query: 177 KLDLVRNETLTCYNGFK-GDGCGHCAACNLRANGLNHYLADKPTVMAAM 224
                   T++C    + G  CG C  C +R    +       T   + 
Sbjct: 316 AFVAQAVRTVSCGKWKRDGTQCGRCVPCLIRRASFHAADIADTTGYRSQ 364


>UniRef50_C6NX81 Archaeosine biosynthesis protein queC n=1 Tax=Acidithiobacillus
           caldus ATCC 51756 RepID=C6NX81_9GAMM
          Length = 206

 Score =  114 bits (286), Expect = 2e-24,   Method: Composition-based stats.
 Identities = 46/215 (21%), Positives = 71/215 (33%), Gaps = 24/215 (11%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-AEIDVARELALKLGARAHKV 60
           +R++++ SGG +S T           ++    DYGQR    E    + LA  L     + 
Sbjct: 5   ERSLLLCSGGVESFTLTHAFGTDSG-LYLFFLDYGQRAAAEEWKRVQNLARNLRLERERF 63

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                        L      V    P+   +P    P RN+L +  AA  A  +    V+
Sbjct: 64  ------------DLRDFGDAVGRLRPQRYHVPT---PHRNLLAVAAAANVAESLGRNTVV 108

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G    D     +    F+ AL   +S      +    PL    KA    L      L +
Sbjct: 109 LGTTADDGRTDRNANRAFLSALAATLSSS---GLYLALPLAQYRKAAVIRLGQD---LGV 162

Query: 181 VRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
               + +C  G++   CG C  C  R         
Sbjct: 163 PWEMSYSCIVGYEA-QCGRCPQCIRRREAFREAGL 196


>UniRef50_Q8R5Z5 tRNA-specific 2-thiouridylase mnmA 2 n=12 Tax=Fusobacterium
           RepID=MNMA2_FUSNN
          Length = 343

 Score =  112 bits (279), Expect = 1e-23,   Method: Composition-based stats.
 Identities = 42/226 (18%), Positives = 73/226 (32%), Gaps = 33/226 (14%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHK 59
           MK+ V+  SGG DS+       +Q  EV  +T + + + +  +I+  +++  KLG     
Sbjct: 1   MKKVVIGMSGGVDSSVSAYLLKEQGYEVIGITLNQHLEENSKDIEDTKKVCDKLGIIHEV 60

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           V        + +                +   P+  +   + +   +    A +  AE V
Sbjct: 61  VNIRKDFENIVIKYFLEG--------YNSGKTPSPCIICDDEIKFKILFDIADKYNAEYV 112

Query: 120 ITGVCETDFSGYPDCRDEFVKALNH--------AVSLGMAKDIRFETPLMWIDKAETWAL 171
            TG   T            +K+++            L   K  R   PL    K E   +
Sbjct: 113 ATG-HYTSVEYSEVFSKYLLKSVHSIIKDQSYMLYRLSPDKLERLIFPLKDYSKQEIREI 171

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLADK 217
           A     L +            K D  G C A      G   +L + 
Sbjct: 172 A-----LKIDLEVH------DKKDSQGVCFA----KEGYKEFLKEN 202


>UniRef50_B5XVJ1 Putative uncharacterized protein n=1 Tax=Klebsiella pneumoniae 342
           RepID=B5XVJ1_KLEP3
          Length = 421

 Score =  112 bits (279), Expect = 1e-23,   Method: Composition-based stats.
 Identities = 34/236 (14%), Positives = 69/236 (29%), Gaps = 44/236 (18%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKL-GARAHKVLDV 63
           + + SGG DS    +  + +  +   V+    Q    + +  R  A  L G  +H     
Sbjct: 141 ISLLSGGLDSLIGGIDLVAKGRKPLFVS----QLAHKDSERQRAYASMLMGNGSHYQWSH 196

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE--AVIT 121
            +  + +    TR                      R++ F   A + + ++      +  
Sbjct: 197 GISFKGSREPSTRG---------------------RSLAFYAFALLASTKINLPKPTIFI 235

Query: 122 ---GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
              GV   +    P              F+  L   +   +   ++ E P  +  K E  
Sbjct: 236 PENGVISINPPLVPGRVSSLSTRTTHPMFIDMLQRVLD-NLGIHVQLELPYRFKTKGEMM 294

Query: 170 ALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLAD-KPTVMA 222
                   L     ++ +C  +  +    CG C  C +R      +  +   T   
Sbjct: 295 RECLDQDLLQKWAADSTSCGRFRTYNRQHCGRCVPCLIRRAAFIAWKPEGDTTSYR 350


>UniRef50_C5PNW4 ExsB superfamily protein n=1 Tax=Sphingobacterium spiritivorum ATCC
           33861 RepID=C5PNW4_9SPHI
          Length = 198

 Score =  111 bits (278), Expect = 2e-23,   Method: Composition-based stats.
 Identities = 41/214 (19%), Positives = 75/214 (35%), Gaps = 21/214 (9%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-RHRAEIDVARELALKLGARAHKVL 61
           +  ++ SGG DS                 T +YGQ     EI V+R +   L  + HKV+
Sbjct: 5   KQGLLLSGGVDSICLAYGLKPD----IAYTINYGQIPASREIYVSRYICNLLRIQ-HKVI 59

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +V   +  +   +  + + +   +         + P RN L +TLAA+ A + K   +  
Sbjct: 60  EVNCKSLGSGILINEERLDISPSQE--------WWPYRNQLLITLAAMQAIKDKIGVLHL 111

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
              ++D S + D   +F   +N  V      +I            E     +   +   +
Sbjct: 112 ASVKSD-SFHKDGTPQFYDLINELVKY-QEGNISIVCESTGYYSHELVKKYNVPLE---I 166

Query: 182 RNETLTCYNGFKGDGCGHCAACNLRANGLNHYLA 215
                +C+       CG C  C  +    +    
Sbjct: 167 IAFAHSCH--ISNTACGQCPGCKKQIKVRHELSI 198


>UniRef50_C7X359 Putative uncharacterized protein n=1 Tax=Enterococcus faecalis
           Merz96 RepID=C7X359_ENTFA
          Length = 427

 Score =  110 bits (275), Expect = 4e-23,   Method: Composition-based stats.
 Identities = 37/249 (14%), Positives = 75/249 (30%), Gaps = 40/249 (16%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDY---GQRHRAEIDVARELALKLGARAHKVL 61
           V +FSGG DS    +  L+     + +   +   G +               G +   V 
Sbjct: 140 VCMFSGGLDSFIGSIDILRGQTTSNTLFVSHYGGGSKGTK------------GYQDLLVK 187

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           ++         S            P     P T    R+  F+  A   A       +I 
Sbjct: 188 EMINHFSSLSLSNFFSFYSAAMKTPTGQDEPTT----RSRSFMFFAHAIALTPDNAKLII 243

Query: 122 ---GVCETDF---------SGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
              G    +          S        ++      +++ +  ++R E P  ++ K +  
Sbjct: 244 PENGFVSLNIPLYFSRNGSSSTRTTHPFYMNLFQKILNI-LGINVRLENPYQFLTKGQMM 302

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGD--------GCGHCAACNLRANGLNHYLADKPTVM 221
                   L      T++C +  +           CG+C  C +R   +N + +  PT+ 
Sbjct: 303 NNCLDKEFLSRNIINTMSCSHPEQARWSGCSDPIHCGYCIPCTIRRASINEFCSPDPTLY 362

Query: 222 AAMKQKTGL 230
             +  +  +
Sbjct: 363 RYLNYQNKI 371


>UniRef50_Q1LLD1 Putative uncharacterized protein n=1 Tax=Cupriavidus metallidurans
           CH34 RepID=Q1LLD1_RALME
          Length = 419

 Score =  108 bits (270), Expect = 1e-22,   Method: Composition-based stats.
 Identities = 40/239 (16%), Positives = 69/239 (28%), Gaps = 44/239 (18%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLG-ARAHKV 60
           +  V + SGG DS    + A+ +      V+    Q    + +  R+ A  LG A  H+ 
Sbjct: 137 RDCVALLSGGLDSLIGGIDAVAKMRRPIFVS----QLAYEDSERQRQYAAALGGAAWHQQ 192

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLA---AIYAYQVKAE 117
               +                   +P A   P+T    R++ F  LA             
Sbjct: 193 WSHRI-------------------DPAAPREPST--RARSMGFYALAVLATSLLTTTGPA 231

Query: 118 AVIT---GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWIDK 165
            ++    G    +    P              F+K L   +   +        P  +  K
Sbjct: 232 EILVPENGFISVNPPLLPGRMASLSTRTTHPLFMKQLQGLLK-AVGIPANLSMPYRFKTK 290

Query: 166 AETWALADYYGKLDLVRNETLTC--YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMA 222
            E          L    +++ +C  Y  +    CG C  C +R      +     T   
Sbjct: 291 GEMMMECLDQKLLAHFASDSTSCGRYRTYNRTHCGRCVPCMVRRAAFLRWGQADDTRYK 349


>UniRef50_A1SFM8 ExsB family protein n=1 Tax=Nocardioides sp. JS614
           RepID=A1SFM8_NOCSJ
          Length = 183

 Score =  108 bits (269), Expect = 2e-22,   Method: Composition-based stats.
 Identities = 49/212 (23%), Positives = 69/212 (32%), Gaps = 40/212 (18%)

Query: 1   MKR----AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGA 55
           MKR      V+ SGG DS   L     +      V  DYGQ     E   +R +A   G+
Sbjct: 1   MKRPATDICVLLSGGVDSALVLAMLSNRS--PVSVWVDYGQPVADVERQASRAIANHFGS 58

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
              +V+   L    +   L                       GRN L +  A   A    
Sbjct: 59  PWMEVVIKGLAPPASGEFL-----------------------GRNDLLVAAALSVA---G 92

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYY 175
              V  G      +GY DC   +  +    +            PL  +DKA+ + LA   
Sbjct: 93  GGFVAIGTHAG--TGYADCSLAWAGSWQALLDAQHGGTAGLLAPLSNLDKAQVYELAVAN 150

Query: 176 GKLDLVRNETLTCYNGFKGDGCGHCAACNLRA 207
           G   +    T +C        CG C++C  R 
Sbjct: 151 G---VPIELTHSCETT--AAPCGSCSSCADRR 177


>UniRef50_A8L9C0 ExsB family protein n=2 Tax=Actinomycetales RepID=A8L9C0_FRASN
          Length = 372

 Score =  107 bits (267), Expect = 3e-22,   Method: Composition-based stats.
 Identities = 39/215 (18%), Positives = 71/215 (33%), Gaps = 32/215 (14%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V++FS G DS            +   + FD G R+R +   A                  
Sbjct: 132 VLLFSAGLDSFPAWHYL----GKPPALYFDLGHRYRDQERAA---------------ITA 172

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
           L++   +       + +  +E +        +P RN+    LAA  A ++    V     
Sbjct: 173 LVDRCGIKVTVSQELDLSQWEADD-----AIIPLRNVYLAMLAANRAERIWCVGV----- 222

Query: 125 ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNE 184
           + D +   D   +    ++  ++    + +  ++P   + K E  A     G        
Sbjct: 223 KGDAT--ADKSPQAFHQISQMITDLSGRPVWLDSPFWQMTKTEIVAWYLGQGLPAGDLLL 280

Query: 185 TLTCYNGFKG-DGCGHCAACNLRANGLNHYLADKP 218
           T +C         CG C +C  R   L +   D P
Sbjct: 281 TFSCSRTDSAMTHCGQCPSCLRRWISLINNGIDAP 315


>UniRef50_A0B2R8 Putative uncharacterized protein n=4 Tax=Burkholderia cenocepacia
           RepID=A0B2R8_BURCH
          Length = 428

 Score =  107 bits (267), Expect = 3e-22,   Method: Composition-based stats.
 Identities = 37/235 (15%), Positives = 62/235 (26%), Gaps = 34/235 (14%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL--- 61
           V + SGG DS    +   ++      V+    Q  + + +     A+ LG     +    
Sbjct: 142 VCLLSGGLDSLVGALDLTEEGRHPLLVS----QTAKGDKETQSRFAIGLGGNDRHLQWNQ 197

Query: 62  -DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNT----------FVPGRNILFLTLAAIY 110
                + ++  S+  R           AD +  T          FVP   ++ L +    
Sbjct: 198 NIRPKVEDIEGSTRGRSIGFFAFAAVAADHLATTITALPRPVEVFVPENGLISLNI---- 253

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
                   +  G               F+  L       +        P   + K E  A
Sbjct: 254 -------PLNPG--RVGSLSTKTTHPVFMARLQALWDE-LGIRAVLRLPYAAMTKGEMMA 303

Query: 171 LADYYGKLDLVRNETLTCYN--GFKGDGCGHCAACNLRANGLNHYLADKPTVMAA 223
                  L  V +E  +C          CG C  C +R            T    
Sbjct: 304 ECRASTVLADVASEATSCGRFVRNGYKHCGRCVPCLVRRASFIRANLRDDTTYVY 358


>UniRef50_Q12C76 Putative uncharacterized protein n=1 Tax=Polaromonas sp. JS666
           RepID=Q12C76_POLSJ
          Length = 466

 Score =  107 bits (266), Expect = 4e-22,   Method: Composition-based stats.
 Identities = 39/242 (16%), Positives = 64/242 (26%), Gaps = 43/242 (17%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           +R  + +S G DS     Q  +       +  +        +  A           H++ 
Sbjct: 147 ERVFMPYSNGLDSFALANQVQRDSSTTELILIN--------VQAA-----------HRLT 187

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY--------- 112
           +   L       +    + V   EP          P    +     A  A          
Sbjct: 188 NWKNLGRARDKDMQAVQVAVFFNEPHRTEPSFRSRPFIYDMLAGYGASLAQPAKVVIPEN 247

Query: 113 ---QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
               +    V  G    D + +  C   F   L   V+      +RFE P ++  K +  
Sbjct: 248 GQGSLGGSLVPLG----DEAPHRSCHPGFTSRLTRLVNALTNVTVRFEHPALFQTKGQVL 303

Query: 170 -ALADYYGKLDLVRNETLTCY-------NGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
             LA      +       +C           +   CG C  C LR   L     +  T  
Sbjct: 304 AQLASQKSASEAWLAGHRSCSYDARHSSQDKRSMHCGVCGNCLLRRVALKWTGIEDTTDY 363

Query: 222 AA 223
            A
Sbjct: 364 RA 365


>UniRef50_A8AM74 Putative uncharacterized protein n=8 Tax=Gammaproteobacteria
           RepID=A8AM74_CITK8
          Length = 446

 Score =  107 bits (266), Expect = 5e-22,   Method: Composition-based stats.
 Identities = 36/241 (14%), Positives = 72/241 (29%), Gaps = 37/241 (15%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V +FSGG DS   ++  L    +   ++  Y    + +     ++A       HK+ +  
Sbjct: 157 VSLFSGGLDSAIGVIDFLSSGRKPLLISHSY----KGDKTKQDQIA-------HKIREKG 205

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ--VKAEAVIT- 121
             + L  S+         D       +        N L   L   YA +     + +   
Sbjct: 206 TFSRLRSSANPIGRGMTRDITMRTRSL--------NFLAFALVGAYAVKQINNHKDLSIF 257

Query: 122 ----GVCETDFS---------GYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAET 168
               G    +                   F+  +    +  +   +R   P  ++ K + 
Sbjct: 258 VPENGFISLNAPLTHRRVGSLSTRTTHPYFIGMIQELFN-NLGFGVRLINPYQFMTKGQM 316

Query: 169 WALADYYGKLDLVRNETLTC-YNGFKGDGCGHCAACNLRANGLNHYLADKPTVMAAMKQK 227
            +       L  + + T++C +   K   CG+C  C +R   L      +          
Sbjct: 317 VSNCKDSKMLSEIVDLTVSCSHWKRKNQQCGYCVPCMIRRAALMKGTLKESISYHHASYP 376

Query: 228 T 228
           T
Sbjct: 377 T 377


>UniRef50_B4U7S9 ExsB family protein n=1 Tax=Hydrogenobaculum sp. Y04AAS1
           RepID=B4U7S9_HYDS0
          Length = 196

 Score =  106 bits (264), Expect = 6e-22,   Method: Composition-based stats.
 Identities = 39/204 (19%), Positives = 75/204 (36%), Gaps = 16/204 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-HRAEIDVARELALKLGARAHKVL 61
           + V + S G DS + L   + +  E++ +    G +    E+   +++   +      + 
Sbjct: 2   KVVSLVSSGFDSISLLNYYINKNYEIYPIYVKSGFKWEEEELKHLKDIIDHISKSYKLIK 61

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            + +    A +    D         E        +P RN+  L  A  YAY V A  +  
Sbjct: 62  PLKV--SHAPNDFFEDFNERAFINDED-----VEIPFRNLDLLISALKYAYAVDANLIAI 114

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G+       + D    F+K +N      M  D + +TP + + K + +        +  +
Sbjct: 115 GI--MGLVAFKDNSYNFIKTINDLF--SMYGDYKVDTPFLGMSKKDVFDRY----FIKEL 166

Query: 182 RNETLTCYNGFKGDGCGHCAACNL 205
            ++   C N   G  CG C+ C  
Sbjct: 167 FDKAFCCMNPTDGKECGVCSKCKE 190


>UniRef50_A6D100 Putative uncharacterized protein n=1 Tax=Vibrio shilonii AK1
           RepID=A6D100_9VIBR
          Length = 372

 Score =  105 bits (262), Expect = 1e-21,   Method: Composition-based stats.
 Identities = 43/258 (16%), Positives = 76/258 (29%), Gaps = 52/258 (20%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-----DYGQRHRAEIDVARELALKLGARA 57
           +AV + SGG DS    +  L    ++  +       D  Q ++ +++ A         + 
Sbjct: 47  KAVSLLSGGLDSFIGAIDLLN--SDIDTLFVGHYAKDGTQHYQNQVEQA--------LKG 96

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK-- 115
           H               L  D         E D   +     R+ +FL++AA  A  +   
Sbjct: 97  HY---PDYFKGYVKGCLIFDKSVFKGGNQEIDSENSQ--RSRSFMFLSMAAYLASSMGEL 151

Query: 116 AEAVIT--GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWID 164
            + ++   G    +    P              F+  +       +  +I    P   + 
Sbjct: 152 CKLIVPENGFIALNVPLDPLRIGANSTKTVHPNFMSKMGEIFDF-LGLNIGLINPYRHMT 210

Query: 165 KAETWALADYYGKLDLVRNETLTCYNGFKGDG-CG-----------------HCAACNLR 206
           K E  A       L    +ETL+C +  K     G                 +C  C +R
Sbjct: 211 KGEMMANCKNKSLLTKHAHETLSCASQSKMRWDQGGFKANRLGGSGGNGNCGYCYPCLIR 270

Query: 207 ANGLNHYLADKPTVMAAM 224
                  L    T   A+
Sbjct: 271 KASFQKALITDNTEYVAI 288


>UniRef50_A6Q239 tRNA-specific 2-thiouridylase mnmA n=2 Tax=Epsilonproteobacteria
           RepID=MNMA_NITSB
          Length = 339

 Score =  104 bits (259), Expect = 3e-21,   Method: Composition-based stats.
 Identities = 37/190 (19%), Positives = 58/190 (30%), Gaps = 12/190 (6%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARAH 58
           K+ VV  SGG DS+        +  EV  V   +  R   H+  I   +++A  LG   H
Sbjct: 3   KKIVVAMSGGVDSSYTANLLQSRGYEVIGVYMKFHPREEYHQKNIANIQKVAKHLGIEYH 62

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            +       E                   A   PN       ++  T    +A ++  + 
Sbjct: 63  VLDRTKEFQERVYQPFVDS--------YVAGLTPNPCAMCNRVMKFTELIEFADELGVDN 114

Query: 119 VITGVCE-TDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           V TG    TD     +  D+         +L      R   PL    K      A     
Sbjct: 115 VATGHYAKTDGQFIYEATDKSKDQSYFLFNLKKEFLPRIIFPLGDWYKENVKKEAMKILL 174

Query: 178 LDLVRNETLT 187
           L  +  +  +
Sbjct: 175 LKSIAEQKES 184


>UniRef50_Q9UWQ2 Putative thiamin biosynthesis protein thiI n=1 Tax=Pyrococcus
           furiosus RepID=Q9UWQ2_PYRFU
          Length = 363

 Score =  104 bits (259), Expect = 3e-21,   Method: Composition-based stats.
 Identities = 34/191 (17%), Positives = 62/191 (32%), Gaps = 20/191 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + SGG DS       L++  EV  + FD G   +  ++   E+         +++ 
Sbjct: 167 KVVALISGGIDSPVAAFLMLKRGAEVIALHFDQGTNAKKVVEKVVEILSDYSPEPIELIV 226

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                 L         +   ++      I            L  AA  A +  A  ++TG
Sbjct: 227 ENHFEILKPYVSVLAKLQKREWTCILCKIA----------MLKRAAEIAKEEGALGIVTG 276

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L++     ++ D     PL+  DK E  ++A   G  D   
Sbjct: 277 DSLGQVAS---------QTLSNLFIETISVDYPIYRPLIGFDKEEIVSIAKRIGTYDAFL 327

Query: 183 NETLTCYNGFK 193
                C   F+
Sbjct: 328 EYPY-CECPFR 337


>UniRef50_Q0AM89 Putative uncharacterized protein n=1 Tax=Maricaulis maris MCS10
           RepID=Q0AM89_MARMM
          Length = 439

 Score =  103 bits (257), Expect = 5e-21,   Method: Composition-based stats.
 Identities = 34/218 (15%), Positives = 62/218 (28%), Gaps = 13/218 (5%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V +FSGG DS    +  +        V+       R +      L   L       ++  
Sbjct: 149 VCLFSGGMDSLIGAIDRIADGHRPLLVSRST----RGDGGYQTYLHGMLPEVPSLRINDD 204

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI--TG 122
           +  EL     TR    V            + +   +I+ L +       +     +   G
Sbjct: 205 IRGELQGEPSTRTRSIVFLALAACAASAISRLHNDSIVPLIIPENGFISLNPPLTVRRIG 264

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
              T           F+ +L       +      E P  +  K E          +  + 
Sbjct: 265 ALST-----RTVHPHFISSLQAIFDE-VGIPAAIENPYFFKTKGEALRECRDQPLVTKLI 318

Query: 183 NETLTCYNGFKG-DGCGHCAACNLRANGLNHYLADKPT 219
           ++T +C    +    CG C  C +R +  +       T
Sbjct: 319 SKTTSCGKWKRYWQQCGRCVPCLIRRSAFHAAGIADET 356


>UniRef50_A4T1U6 Putative uncharacterized protein n=2 Tax=Actinomycetales
           RepID=A4T1U6_MYCGI
          Length = 454

 Score =  102 bits (254), Expect = 1e-20,   Method: Composition-based stats.
 Identities = 45/242 (18%), Positives = 81/242 (33%), Gaps = 34/242 (14%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDE--VHCVTFDYG-QRHRAEIDVARELALKLGARAHKV 60
           AV +FSGG DS T ++  L++  +  +  ++ + G Q   A+  +   L    G    ++
Sbjct: 144 AVSLFSGGLDSLTGVIDLLEEDPDRRLCLISHNEGDQASTAQKTLTAALREHYGQDRLEL 203

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA-V 119
                L      ++   + P+P           T    R++LFLT     A  V  +  V
Sbjct: 204 RS---LFLRPAPAVAGQARPLPPERE-------TTTRARSLLFLTAGLALAASVGPDVPV 253

Query: 120 IT---GVCETDF---------SGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
                G    +                  +F++ L  A    +        P     K E
Sbjct: 254 YIPENGFIGINVPLTRARSGSYSTRTTHPQFMQLLRTAAD-AVGVCNPILNPFRLKTKGE 312

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGD-------GCGHCAACNLRANGLNHYLADKPTV 220
             A +     L  +   +++C +             CG+C  C +R + +     D P  
Sbjct: 313 ILASSRNPELLRSLAPNSISCSHPEAARYAVRKQGNCGYCFPCLIRRSSMARVGWDSPEG 372

Query: 221 MA 222
            A
Sbjct: 373 YA 374


>UniRef50_B5YCZ9 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=2
           Tax=Dictyoglomus RepID=B5YCZ9_DICT6
          Length = 345

 Score =  102 bits (253), Expect = 1e-20,   Method: Composition-based stats.
 Identities = 38/201 (18%), Positives = 67/201 (33%), Gaps = 18/201 (8%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +  V  SGG DS+T      ++  EV  +T +    +   I  A ++A  L      V  
Sbjct: 4   KVAVGMSGGVDSSTTAYLLKKEGYEVIGLTMNIA--YSDSIKDAEKIAKYLNIPHFYVDL 61

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                E  +               ++   PN       I+   L    A ++ AE + TG
Sbjct: 62  RKEFEEQIIKYFIDS--------YKSGYTPNPCTFCNRIIKFGLLLKKAQELGAEYLATG 113

Query: 123 -----VCETDFSGYPDCRDEFVKALNH--AVSLGMAKDIRFETPLMWIDKAETWALADYY 175
                V + +   +   R + +K         L   +  +   PL  + K E   LA+  
Sbjct: 114 HYVRKVWDEEKKVFLLKRAKDLKKDQSYFLAYLTQEQIEKSLFPLGDLTKNEVRKLAEEI 173

Query: 176 GKLDLVRNETLT-CYNGFKGD 195
           G     + E+   C+   +  
Sbjct: 174 GLFVSDKEESQDICFLPDRDY 194


>UniRef50_Q5JCY1 Probable tRNA sulfurtransferase n=14 Tax=Thermococcaceae
           RepID=THII_PYRKO
          Length = 381

 Score =  101 bits (251), Expect = 2e-20,   Method: Composition-based stats.
 Identities = 32/178 (17%), Positives = 62/178 (34%), Gaps = 19/178 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + SGG DS       +++  EV  V    G      ++  R++  +L  + H    
Sbjct: 182 KVVALLSGGIDSPVAAFLMMKRGVEVIPVHIYMG---EKTLEKVRKIWNQLK-KYHYGGK 237

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
             L+     +        +     E      T V  +  + +  A   A +  A+ ++ G
Sbjct: 238 AELIVVKPQN-----REEMLKKIKELGKEKYTCVLCK-FMMVKHADRIAKEFGAKGIVMG 291

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
                 +          + L +   +  A D+    PL+ +DK E   +A   G  +L
Sbjct: 292 DSLGQVAS---------QTLENMYIVSQASDLPIYRPLIGLDKEEIVDIAKKIGTFEL 340


>UniRef50_A5GE36 tRNA-specific 2-thiouridylase mnmA n=20 Tax=Bacteria
           RepID=MNMA_GEOUR
          Length = 375

 Score =  100 bits (250), Expect = 3e-20,   Method: Composition-based stats.
 Identities = 42/198 (21%), Positives = 65/198 (32%), Gaps = 31/198 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF---DY-------GQRHR-----AEIDVA 46
           KR V+  SGG DS+       ++  EV  ++    DY       G++        +I  A
Sbjct: 8   KRVVIAMSGGVDSSVAAALLKEEGHEVIGISMQVWDYTRFTAEEGEKFDTCCSLDDIHDA 67

Query: 47  RELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTL 106
           R +A +L    + V        L +             E      PN  V     +   L
Sbjct: 68  RRVAEQLEIPFYVVNFEEEFQNLVIDDFV--------NEYLLGRTPNPCVRCNQWIKFEL 119

Query: 107 AAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETP 159
               A  + A+ + TG          D R   +K ++          +L   +      P
Sbjct: 120 LLKKARGLGADMIATG-HYARTEQDADGRYRLMKGVDPAKDQSYFLFTLTQEQLAMTLFP 178

Query: 160 LMWIDKAETWALADYYGK 177
           L  + K E  ALA  YG 
Sbjct: 179 LGGMTKQEVRALAAGYGL 196


>UniRef50_B2IBH3 tRNA-specific 2-thiouridylase mnmA n=141 Tax=root RepID=MNMA_BEII9
          Length = 431

 Score =  100 bits (250), Expect = 3e-20,   Method: Composition-based stats.
 Identities = 40/201 (19%), Positives = 66/201 (32%), Gaps = 27/201 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF---DYGQ--------RHRAEIDVARELAL 51
           R VV  SGG DS+       ++  EV  VT    D+G+            +I  AR++A 
Sbjct: 31  RVVVAMSGGVDSSVVAALLKREGYEVIGVTLQLYDHGEAVHRKGACCAGQDIQDARQVAD 90

Query: 52  KLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           +LG   H VLD             R  I            P   V   + +        A
Sbjct: 91  QLGI-PHYVLDYEERFR-------RKVIDPFAQSYATGETPIPCVSCNSEIKFADLFETA 142

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWID 164
             + A+ + TG          D      ++L+          +   A+      PL  + 
Sbjct: 143 QHLGADVLATG-HYVSSKAEADGGRALYRSLDSSRDQSYFLFATTNAQLELLRFPLGDLA 201

Query: 165 KAETWALADYYGKLDLVRNET 185
           K +   +A   G +   + ++
Sbjct: 202 KPDVRQMARQLGLVVADKADS 222


>UniRef50_B5YGH6 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Thermodesulfovibrio yellowstonii DSM 11347
           RepID=B5YGH6_THEYD
          Length = 342

 Score =  100 bits (249), Expect = 4e-20,   Method: Composition-based stats.
 Identities = 34/193 (17%), Positives = 62/193 (32%), Gaps = 18/193 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M++ ++  SGG DS+       ++   V  V F       A I++A++ A  L  + H  
Sbjct: 1   MEKVLLAMSGGIDSSVAAYLLREKGMAVEGVFFIL-FDEPANIELAKQTANFLNIKLHIE 59

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                     ++              +    PN  V     +   L    A +VKA  + 
Sbjct: 60  DLREKFKADVITPFFEG--------YKKGITPNPCVICNKKIKFPLLKFLAEKVKANYIA 111

Query: 121 TGVCETDFSGYPDCRDEFVKAL-----NHAVSLGMAKDI--RFETPLMWIDKAETWALAD 173
           TG          +     +K +           G+ + +      PL    K +   +A+
Sbjct: 112 TGHYA--RIVRINNIPLLMKGIDPKKDQSYFLYGIERTLLSHIIFPLGEYTKYKIREIAE 169

Query: 174 YYGKLDLVRNETL 186
             G    V  E+ 
Sbjct: 170 KVGIPSKVAEEST 182


>UniRef50_C7N321 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Slackia heliotrinireducens DSM 20476
           RepID=C7N321_SLAHD
          Length = 353

 Score = 99.7 bits (247), Expect = 7e-20,   Method: Composition-based stats.
 Identities = 38/203 (18%), Positives = 66/203 (32%), Gaps = 27/203 (13%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-----------DYGQRHRAEIDVAREL 49
           ++R +V  SGG DS+      L+Q  EV   T            D       +++ AR +
Sbjct: 2   VQRVLVGMSGGVDSSVTAYLLLRQGYEVVGATMNLYDPGRLGVPDVRDGESNDLEDARSV 61

Query: 50  ALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
           A +LG   H +                + +            PN  +     L   L   
Sbjct: 62  ANRLGIAHHVLDFGCEFEN--------NVVRPFAQAYADGLTPNPCIACNRHLKFGLMLK 113

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLG-------MAKDIRFETPLMW 162
            A ++  + + TG      +   D R   +K L+ A             +      PL  
Sbjct: 114 AARELGCDGIATG-HYARIAQRLDGRFVLMKGLDDAKDQAYVLYHMTQDQLAHTVLPLGE 172

Query: 163 IDKAETWALADYYGKLDLVRNET 185
             K +  A+A   G  +  ++E+
Sbjct: 173 YTKQQVRAIAAEQGFANARKHES 195


>UniRef50_A0YNH9 Putative uncharacterized protein n=2 Tax=Oscillatoriales
           RepID=A0YNH9_9CYAN
          Length = 488

 Score = 99.4 bits (246), Expect = 8e-20,   Method: Composition-based stats.
 Identities = 40/233 (17%), Positives = 71/233 (30%), Gaps = 32/233 (13%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVT 64
           V ++SGG D+   L   LQ    V  + F  G          +     + +    +    
Sbjct: 158 VALWSGGLDALAGLYTRLQTSHTVPFMLFGTGSSKSVYARQEKVFQAIIKSFPTHLYLCR 217

Query: 65  LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGVC 124
           +      S L R                N     R ++F  L A  AY +    +     
Sbjct: 218 VPINFLNSKLLR---------------KNKITRARGVVFTLLGAACAYLMGQRTLSVYEN 262

Query: 125 ------------ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                                     +  ++  VS  + ++ R   P +++ KA+     
Sbjct: 263 GIGAINLPYRASAVGLDHSRSVHPLTLLMVSDVVSELLGENFRVYNPFLFLTKAQICRAL 322

Query: 173 DYYGKLDLVRNETLTCYNGFKG----DGCGHCAACNLRANGLNHYLADKPTVM 221
               + DLV   T +C +  +       CG+C++C LR   L     +  T  
Sbjct: 323 AENQENDLV-AITQSCDSRHRKSQQPTQCGYCSSCILRKQALAASKIEDKTRY 374


>UniRef50_C4ZB38 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=3
           Tax=Firmicutes RepID=C4ZB38_EUBR3
          Length = 362

 Score = 99.0 bits (245), Expect = 1e-19,   Method: Composition-based stats.
 Identities = 37/216 (17%), Positives = 72/216 (33%), Gaps = 30/216 (13%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-------------IDVARE 48
           K+ VV  SGG DS+       +Q  +V  VT    Q                  ++ AR 
Sbjct: 3   KKVVVGMSGGVDSSVAAYLLKEQGYDVIGVTMQIWQDEDVFKQSQEGGCCGLSAVEDARR 62

Query: 49  LALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
           +A  L    + V++     +  V            Y P      N +V   ++L  +L  
Sbjct: 63  VAAVLDI-PYYVMNFKADFKKYVIDYFVSEYE-RAYTPNPCIACNRYVKWESLLHRSL-- 118

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALN-------HAVSLGMAKDIRFETPLM 161
               ++ A+ + TG      +  P+ R     ++           +L   +  R   P+ 
Sbjct: 119 ----EIGADYIATG-HYARIAQLPNGRYTIANSVTAEKDQTYALYNLTQEQLRRTLMPVG 173

Query: 162 WIDKAETWALADYYGKLDLVRNETLT-CYNGFKGDG 196
             +K +   +A+  G     ++++   C+       
Sbjct: 174 EYEKPQIRRIAEEAGLPVAHKSDSQDICFVPDHDYA 209


>UniRef50_C6MK22 Putative uncharacterized protein n=2 Tax=Deltaproteobacteria
           RepID=C6MK22_9DELT
          Length = 700

 Score = 98.6 bits (244), Expect = 2e-19,   Method: Composition-based stats.
 Identities = 43/240 (17%), Positives = 82/240 (34%), Gaps = 41/240 (17%)

Query: 5   VVVFSGGQDSTTCLV--QALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           VV+FSGG DS    +     +++  V        + ++    + + +A K G  A   + 
Sbjct: 157 VVMFSGGLDSLAGAIDEVLNEKHRVVLVTHKSTPKLNKRHRRLEKMIADKAGENAPLHIG 216

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT- 121
           V +     ++           YE             R+ L++++ A  A  +  ++V   
Sbjct: 217 VRVNKNKGLN-----------YEYTQRS--------RSFLYVSIGATIAKMINLKSVRFY 257

Query: 122 --GVCETDFSG---------YPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
             GV   +                    ++     +SL   +    E P +W  KA+   
Sbjct: 258 ENGVISLNLPVCAQVVGGRATRTTHPRVIRGFQEIISLVAGEPFTIENPYIWKTKADVVG 317

Query: 171 LADYYGKLDLVRNETLTCYNGFK----GDGCGHCAACNLRANGLNHYLAD--KPT-VMAA 223
           +    G  +++   T TC + ++       CG C+ C  R   +    AD   P     A
Sbjct: 318 VITKAGCQEMIAAST-TCTHTWEMTNHHTHCGTCSQCIDRRFAVIAAKADQHDPVDAYKA 376


>UniRef50_Q9V1S6 ThiI thiamin biosynthesis protein thiI n=3 Tax=Pyrococcus
           RepID=Q9V1S6_PYRAB
          Length = 198

 Score = 98.2 bits (243), Expect = 2e-19,   Method: Composition-based stats.
 Identities = 35/187 (18%), Positives = 67/187 (35%), Gaps = 20/187 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + S G DS   +     +  EV+ + F   Q    E  V R +         KV D
Sbjct: 2   KVVALLSSGIDSPVAIHLVASKGAEVYPLHFK--QDDVKENKVRRIVKRLQEIHGDKVKD 59

Query: 63  VTLLNELA-VSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
             +++       +      +   +        T           +A  YA ++KA+A++T
Sbjct: 60  PRIVDAFEVQGPVFEKLREIRKEKWTCIFCKYT--------MYIVATSYAREIKAKAIVT 111

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G      +          + L++ + +  A ++    PL+ +DK E   +A   G  D+ 
Sbjct: 112 GDSLGQVAS---------QTLDNLMVMSTATELPILRPLIALDKEEIVRIAKEIGTFDIS 162

Query: 182 RNETLTC 188
             +   C
Sbjct: 163 TEKEPPC 169


>UniRef50_A0PSW2 Putative uncharacterized protein n=1 Tax=Mycobacterium ulcerans
           Agy99 RepID=A0PSW2_MYCUA
          Length = 420

 Score = 98.2 bits (243), Expect = 2e-19,   Method: Composition-based stats.
 Identities = 40/242 (16%), Positives = 59/242 (24%), Gaps = 46/242 (19%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           +  AV + SGG DS +  V    +      ++          +  A+             
Sbjct: 133 VPDAVSLLSGGLDSLSGAVL---KGAPGLFLSHT----DNPTVTGAQR------------ 173

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV- 119
                      S+              +    NT    R +LF  LA   A     + V 
Sbjct: 174 ----RTWNWLTSNGVEGECVRLSLNEASRKRENTT-RTRALLFYALAVALADARGIDRVE 228

Query: 120 -----ITGVC------ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAET 168
                 TG+                     +  +   +      DI    P  W  K E 
Sbjct: 229 VSENGFTGLNLSLGNDRGGVLSTRSTHPWTMHLMQRLLDDA-GIDIELVNPYEWQTKGEL 287

Query: 169 WALADYYGK-LDLVRNETLTC-------YNGFK-GDGCGHCAACNLRANGLNHYLADKPT 219
              A             TL+C       Y G      CG C AC  R   +     +  T
Sbjct: 288 VRAAADVCPAFAEGLVTTLSCAKLDGRTYKGGNPNLNCGLCVACLTRRASIRAAGLEDKT 347

Query: 220 VM 221
             
Sbjct: 348 PY 349


>UniRef50_C7RPK0 Putative uncharacterized protein n=1 Tax=Candidatus Accumulibacter
           phosphatis clade IIA str. UW-1 RepID=C7RPK0_9PROT
          Length = 458

 Score = 97.8 bits (242), Expect = 3e-19,   Method: Composition-based stats.
 Identities = 47/242 (19%), Positives = 79/242 (32%), Gaps = 38/242 (15%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           ++ +FSGG DS    +  LQ       ++  +G+   ++         KL A+  K    
Sbjct: 151 SLCLFSGGLDSLIGAINLLQAESAPLLIS-HFGESATSDAQ------NKLFAKLKKQYAR 203

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPG----------RNILFLTLAAI--YA 111
           +  + L V     D + V D   E      +F+            RN   L +      A
Sbjct: 204 SAFDRLRVGMTFDDGL-VKDVGSENSTRGRSFLFFALGVFAGTGLRNGFTLKVPENGLIA 262

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
             V  + +  G   T           F+   N  +   +  D R E P + + K E    
Sbjct: 263 LNVPLDPLRLGSNST-----RTTHPYFMARWNDLLDE-LGIDGRVENPYLPMTKGEMVLA 316

Query: 172 ADYYGKLDLVRNETLTC----------YNGFKGDGCGHCAACNLRANGLNHYLAD--KPT 219
                 L  +   +L+C          + G   + CG+C  C +R   LN    +    T
Sbjct: 317 CANQKLLLELAPMSLSCAHPANARWQGHKGRGIEHCGYCLPCLIRRAALNRAWGEGGDRT 376

Query: 220 VM 221
             
Sbjct: 377 AY 378


>UniRef50_Q72HP1 Probable tRNA sulfurtransferase n=3 Tax=Thermus RepID=THII_THET2
          Length = 406

 Score = 97.8 bits (242), Expect = 3e-19,   Method: Composition-based stats.
 Identities = 37/177 (20%), Positives = 59/177 (33%), Gaps = 22/177 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHKVL 61
           R V + SGG DS     + +++  EV  V F  +     A  + A+ LA +L    H++ 
Sbjct: 176 RVVALLSGGIDSPVAAYRLMRRGAEVVLVHFHPFPLLSGASREKAKALAERLARFQHRLR 235

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                              V  +           V  R    L +A   A +  A A+ T
Sbjct: 236 L-----------HLVPFSEVQRHIIVEAPTAYRVVLYRRY-MLRIAEAIAREEGALALCT 283

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           G      +          + L +  ++  A  +    PL+  DK E  A A   G  
Sbjct: 284 GDSLGQVAS---------QTLENLHAVNQAATLPVFRPLIGWDKEEIVAEAQRIGTY 331


>UniRef50_C9RQ01 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Fibrobacter succinogenes subsp. succinogenes S85
           RepID=C9RQ01_FIBSS
          Length = 366

 Score = 97.4 bits (241), Expect = 4e-19,   Method: Composition-based stats.
 Identities = 45/204 (22%), Positives = 63/204 (30%), Gaps = 35/204 (17%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-----------------DYGQRHRAEID 44
           KR  V  SGG DS        +Q  EV  +T                   YG      I+
Sbjct: 5   KRVAVGLSGGVDSALSAYLLKKQGYEVVGMTMATWDDSVKMPAVEGREGCYGPSEDKNIE 64

Query: 45  VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFL 104
            A+ +A +LG   H V+ V            R+ +     E  A   PN  V     +  
Sbjct: 65  EAKLVAERLGI-PHYVVPVAE-------DYKREVLDYFRAEYRAGRTPNPCVRCNQSIKF 116

Query: 105 TLAAIYAYQVKAEAVI--TGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIR 155
                 A ++  +     TG         PD      +AL+           L   +   
Sbjct: 117 GALQHAARKLGIDFDYFATGHYARLDFKNPDV-PFLYEALDEHKDQTYFLSRLSAEQLST 175

Query: 156 FETPLMWIDKAETWALADYYGKLD 179
              PL  + KA+  ALA   G  D
Sbjct: 176 VIFPLGGMQKADVKALAKEIGWDD 199


>UniRef50_UPI0001B54B61 PP-loop superfamily ATPase-like protein n=1 Tax=Streptomyces sp.
           AA4 RepID=UPI0001B54B61
          Length = 423

 Score = 97.0 bits (240), Expect = 4e-19,   Method: Composition-based stats.
 Identities = 43/221 (19%), Positives = 69/221 (31%), Gaps = 33/221 (14%)

Query: 6   VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDVTL 65
           V+FSGG DS+  L   L     +  V+            V   +  +L   + +VL    
Sbjct: 118 VLFSGGLDSSAGLALRL-GGPPLLPVSVRTN---NDMAGVQERVLSRLRGVSREVLPAAS 173

Query: 66  LNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI----- 120
                  +LT      P  + E           R +LFL      A  +    +      
Sbjct: 174 FRV----NLTESRTRAPGAKQENSQ------RSRGLLFLAAGIAVAITLGKGRLWFLENG 223

Query: 121 TGVC-------ETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALAD 173
            G         +             V+A+         +D   ETPL+ + KAE    + 
Sbjct: 224 IGAINLPYLRSQVGSQATRSAHPRTVQAMARLAGTLTGQDFAVETPLLGLTKAEAV--SA 281

Query: 174 YYGKLDLVRNETLTCYNGFKGD-----GCGHCAACNLRANG 209
              + +     +++C  GF         CG C +C LR   
Sbjct: 282 VPPEYEHAIACSVSCDTGFASRISGHPPCGTCTSCLLRRQA 322


>UniRef50_C0B5F7 Putative uncharacterized protein n=2 Tax=Lachnospiraceae
           RepID=C0B5F7_9FIRM
          Length = 359

 Score = 96.3 bits (238), Expect = 8e-19,   Method: Composition-based stats.
 Identities = 37/196 (18%), Positives = 69/196 (35%), Gaps = 29/196 (14%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEI-------------DVARE 48
           K+ VV  SGG DS+       +Q  +V  VT    Q   ++I             D AR+
Sbjct: 4   KKVVVGMSGGVDSSVAAYLLKEQGYDVIGVTMQIWQDEESKIQEENGGCCGLSAVDDARK 63

Query: 49  LALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
           +A ++G   + V++     +  V     D   +    P      N +V   ++L  ++  
Sbjct: 64  VAAQIGI-PYYVMNFKKDFQEKVIDYFVDEY-LHGRTPNPCIACNRYVKWESLLTRSM-- 119

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALN-------HAVSLGMAKDIRFETPLM 161
               ++ A+ + TG         P+ R     +            +L   +  R   P+ 
Sbjct: 120 ----EIGADYIATG-HYARVEQLPNGRYAIRHSATWSKDQTYALYNLTQDQLRRTLMPVG 174

Query: 162 WIDKAETWALADYYGK 177
             +K +   +A   G 
Sbjct: 175 EYEKDQIREIAKEIGL 190


>UniRef50_A3W978 Putative uncharacterized protein n=2 Tax=Rhodobacteraceae
           RepID=A3W978_9RHOB
          Length = 446

 Score = 95.5 bits (236), Expect = 1e-18,   Method: Composition-based stats.
 Identities = 40/233 (17%), Positives = 68/233 (29%), Gaps = 27/233 (11%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHK--VLD 62
           V +FSGG DS    +  L        V+  Y      E   A+   L++    H      
Sbjct: 147 VSLFSGGLDSLIGAIDLLASGQRPLLVS-HYW---DGEASSAQRQLLEVLKERHDGAFES 202

Query: 63  VTLLNELAVSSLTR-----DSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           V        S         +             +    V   N + +    + A  V  +
Sbjct: 203 VRAYIGFRKSDFAEAGSDNNQRARSFLFYSLAVVAADAVASTNKVLIPENGLIALNVPMD 262

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A   G   T  +        F+++++  V   +  D+  E P  +  K E          
Sbjct: 263 AHRLGSLSTRTA-----HPHFIRSMSQLV-TALGVDVTLENPYRFKTKGEMARECGDRDL 316

Query: 178 LDLVRNETLTCYNGFKGD-------GCGHCAACNLRANGLNHYLAD--KPTVM 221
           L  +   +++C +  +          CG C  C +R   L     D    T  
Sbjct: 317 LAELAPVSMSCSHPAQVRFESAPPQHCGRCVPCLIRRASL-AVGLDEADTTKY 368


>UniRef50_D0XY41 Putative uncharacterized protein n=1 Tax=Caulobacter segnis ATCC
           21756 RepID=D0XY41_9CAUL
          Length = 458

 Score = 95.5 bits (236), Expect = 1e-18,   Method: Composition-based stats.
 Identities = 36/232 (15%), Positives = 63/232 (27%), Gaps = 40/232 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + ++ +S G DS        +                 +++   R  A     ++H    
Sbjct: 143 KTIIPYSNGLDSLAVAALTSEA--------------RPSDLVRVRLGAAFADQKSH---- 184

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                     +       V   +             +  +   +AA+ A   K     +G
Sbjct: 185 -----NKRRQAFASVPYEVRVAKGRRKESSARSRGFKFAVITGIAAVLAKADKIVVTESG 239

Query: 123 --------VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADY 174
                                  F   +    +  +     ++ P +W  K ET A A  
Sbjct: 240 QGALGPVLTVSGQAYPDYRVHPAFTMRVERLFATLLGHSAHYDFPRLWSTKGETIAEAAR 299

Query: 175 YGKLDLVRNETLTCYN-------GFKGDGCGHCAACNLRANGLNHYLADKPT 219
                    ET +C+        G K   CG CAAC LR   L+     +P 
Sbjct: 300 ATPQ--PWAETRSCWQQSRQVGMGGKQRQCGVCAACMLRRMSLHAAGVVEPA 349


>UniRef50_UPI00016C4B95 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Gemmata obscuriglobus UQM 2246 RepID=UPI00016C4B95
          Length = 357

 Score = 95.5 bits (236), Expect = 1e-18,   Method: Composition-based stats.
 Identities = 35/198 (17%), Positives = 59/198 (29%), Gaps = 28/198 (14%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID---------------- 44
           M R V+  SGG DS+   V   +Q  EV  +    G     + D                
Sbjct: 1   MARVVLAMSGGVDSSASAVLLKKQGHEVIGLFMRTGTHGHDQPDERRDNKKGCCSAIDAG 60

Query: 45  VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFL 104
            AR +A KL    + +   +  N +         I     E      PN  V   N L  
Sbjct: 61  DARRVADKLDIPFYALDFESEFNRI---------IDYFADEYVRGRTPNPCVVCNNWLKF 111

Query: 105 TLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMW 162
                +  ++ A+ + TG            +          V  G+ +++      P+  
Sbjct: 112 GQLWTFGQKLGADFIATG-HYAQVRNGELHKGVDADKDQSYVLHGIKREVLPHLLFPVGG 170

Query: 163 IDKAETWALADYYGKLDL 180
             K +    A     + +
Sbjct: 171 YTKPQIRQFAREASLVGV 188


>UniRef50_B0TFA7 tRNA-specific 2-thiouridylase mnmA n=97 Tax=Bacteria
           RepID=MNMA_HELMI
          Length = 385

 Score = 95.1 bits (235), Expect = 2e-18,   Method: Composition-based stats.
 Identities = 41/196 (20%), Positives = 62/196 (31%), Gaps = 27/196 (13%)

Query: 1   MK-RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-----------IDVARE 48
           MK   VV  SGG DS+      L+Q  +V  VT     +   E           ++ A+ 
Sbjct: 1   MKETVVVAMSGGVDSSVTAALLLEQGYDVIGVTLQIWPKDAPEGAEGGCCSLSAVEDAKR 60

Query: 49  LALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
           +A KLG   H VL+     E        + I     E  A   PN  +    ++      
Sbjct: 61  VASKLGV-PHYVLNFRDYFE-------TEVIEYFGREYLAGQTPNPCIRCNRVIKFEGLL 112

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALN-------HAVSLGMAKDIRFETPLM 161
             +  + A  V TG            R    + ++          ++   +  R   PL 
Sbjct: 113 QKSLALGAAKVATGHYARIEQDSATGRYRLGRGIDANKDQSYALFNMTQEQLSRTLFPLG 172

Query: 162 WIDKAETWALADYYGK 177
              K E    A   G 
Sbjct: 173 GFTKPEIREKAAQLGL 188


>UniRef50_D0X1F3 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Vibrio alginolyticus 40B RepID=D0X1F3_VIBAL
          Length = 430

 Score = 95.1 bits (235), Expect = 2e-18,   Method: Composition-based stats.
 Identities = 32/210 (15%), Positives = 68/210 (32%), Gaps = 25/210 (11%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE--------IDVARELALKL 53
           K+ +V  SGG DS+       QQ  +V  +     +    E        +  A+ +  KL
Sbjct: 63  KKVIVGMSGGVDSSVSAYLLKQQGYQVEGLFMKNWEEDDNEEYCTAAEDLADAQAVCDKL 122

Query: 54  GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA-Y 112
           G   H +                +       E +A   PN  +     +       +A  
Sbjct: 123 GIHLHTINFAAEYW--------DNVFEYFLAEYKAGRTPNPDILCNKEIKFKAFLEFADE 174

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDK 165
            + A+ +  G            + + ++ L+          +L   +  R   P+  ++K
Sbjct: 175 VLDADYIAMGHYVRRSFPENGEKPQMLRGLDGNKDQSYFLYTLSNEQIARSLFPVGDLEK 234

Query: 166 AETWALADYYGKLDLVRNETLT-CYNGFKG 194
            E   +A+    +   + ++   C+ G + 
Sbjct: 235 PEVRRIAEEQDLITAKKKDSTGICFIGERK 264


>UniRef50_B9JNY0 Putative uncharacterized protein n=1 Tax=Agrobacterium radiobacter
           K84 RepID=B9JNY0_AGRRK
          Length = 457

 Score = 95.1 bits (235), Expect = 2e-18,   Method: Composition-based stats.
 Identities = 41/238 (17%), Positives = 61/238 (25%), Gaps = 51/238 (21%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG----QRHRAEIDVARELALKLGARAH 58
           + V  FS G DS                +    G     R R E   A            
Sbjct: 142 KVVTPFSDGLDSRAVTAMMTAGGRSDRLIRVRVGAKTDDRPRTEHRKA------------ 189

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
                              S   P  E        + V  R   F  L+ I AY +    
Sbjct: 190 ---------PFLTIPFKVKSGDRPFAE--------STVRSRGFKFTLLSGIAAYLLGTHD 232

Query: 119 VI--------TGVCETDFSGYPD---CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
           ++         G      S   +       F   +   V+      + +  P +W  K +
Sbjct: 233 IVMPESGQGALGPSLVQTSHGYEDYRNHPLFFGRMERLVAAIFNYQVSYRLPRLWKTKGQ 292

Query: 168 TWALADYYGKLDLVRNETLTCYN-------GFKGDGCGHCAACNLRANGLNHYLADKP 218
           T               +T +C+          K   CG CAAC LR    +    ++P
Sbjct: 293 TLGEFAALPVNPNEWVKTRSCWQQNRNSSVDGKRRQCGICAACMLRRLSFHAAGLEEP 350


>UniRef50_B1VAX7 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Bacteria
           RepID=MNMA_PHYAS
          Length = 375

 Score = 94.3 bits (233), Expect = 3e-18,   Method: Composition-based stats.
 Identities = 37/219 (16%), Positives = 71/219 (32%), Gaps = 33/219 (15%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH---------------RAEIDV 45
           MK+ VV  SGG DS    +   +   EV  V                         E+D 
Sbjct: 1   MKKVVVGLSGGVDSAVSALLLKKAGYEVEAVFMRNWDSQLNFDFQGNPTLNDVCPQELDY 60

Query: 46  --ARELALKLGARAHKVLDVTLLNE----LAVSSLTRDSIPVPDYEPEADGIPNTFVPGR 99
             A +++L+LG + H+V  +    +      +++   +  P PD     +     F+   
Sbjct: 61  KDALKVSLQLGIKLHRVNFIEEYWQKVFLYFINAFKNNLTPNPDILCNNEIKFKAFINYA 120

Query: 100 NILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDE---FVKALNHAVSLGMAKDIRF 156
                      A    A  +     +   +   D   +   F+  L        ++    
Sbjct: 121 TSKLKPQY--IAMGHYARLIYDKNQKVSLACPLDKNKDQTYFLSQLK------TSQLKNI 172

Query: 157 ETPLMWIDKAETWALADYYGKLDLVRNETLT-CYNGFKG 194
             PL  + K E   +A   G ++  + ++   C+ G + 
Sbjct: 173 LFPLADLTKKEVRKIALENGLINACKKDSTGICFIGERN 211


>UniRef50_O29382 Probable tRNA sulfurtransferase n=2 Tax=Archaeoglobaceae
           RepID=THII_ARCFU
          Length = 374

 Score = 94.3 bits (233), Expect = 3e-18,   Method: Composition-based stats.
 Identities = 37/181 (20%), Positives = 69/181 (38%), Gaps = 22/181 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEI-DVARELALKLGARAHKVL 61
           + V + SGG DS      A+++  EV  V F     H  ++ +  R LA KL     K+ 
Sbjct: 167 KVVALISGGIDSPVAAFMAMKRGCEVVAVHFFNQTMHSPKVREKIRMLAEKLAEYQGKI- 225

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                 +L +         + +  P    +    V  R  +   +A + A +   +AV+T
Sbjct: 226 ------KLYMVPFKEVQFEIINRVPPKLRM----VVYRRSMM-RMANLIAEKEGCKAVVT 274

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G   +  +          + L++   +  A  +    PL+ +DK E  A+A      ++ 
Sbjct: 275 GDSLSQVAS---------QTLDNINVIYSASKLAVLPPLIGMDKEEIVAIAKRIETYEIS 325

Query: 182 R 182
            
Sbjct: 326 I 326


>UniRef50_Q5LIS5 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Bacteroides
           RepID=MNMA1_BACFN
          Length = 364

 Score = 94.0 bits (232), Expect = 4e-18,   Method: Composition-based stats.
 Identities = 51/241 (21%), Positives = 80/241 (33%), Gaps = 38/241 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAE-IDVARELALKLGARAHK 59
           KR ++  SGG DS+   +  L+   EV  VTF  Y      E ++ AR LA +LG   H 
Sbjct: 6   KRVLLGMSGGTDSSVAAMLLLEAGYEVTGVTFRFYEFNGSTEYLEDARALAARLGI-GHI 64

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
             D   + +  +     D       E  +   P       N L   L A  A ++    +
Sbjct: 65  TYDARKVFQEQIIDYFID-------EYMSGHTPVPCTLCNNQLKWPLLAKIADEMGIFYL 117

Query: 120 ITG------VCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETWAL 171
            TG        + ++   P    E V         G+ ++I  R   P+  + K+E  A 
Sbjct: 118 ATGHYVRKQWIDGNYYIAP---AEDVDKDQSFFLWGLRQEILQRMLLPMGGMTKSEARAY 174

Query: 172 ADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLN------HYLADKPTVMAAMK 225
           A   G   +            K D  G C  C L                    +   ++
Sbjct: 175 AAGRGFEKV----------SKKKDSIGVCF-CPLDYRSFLKKCLCDESGDKNRNIYRKVE 223

Query: 226 Q 226
           +
Sbjct: 224 R 224


>UniRef50_B9L3Q7 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Thermomicrobia (class) RepID=B9L3Q7_THERP
          Length = 407

 Score = 93.6 bits (231), Expect = 4e-18,   Method: Composition-based stats.
 Identities = 39/176 (22%), Positives = 61/176 (34%), Gaps = 21/176 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHKVL 61
           R V + SGG DS     + +++   V  V F  Y       I+ A ELA  L        
Sbjct: 182 RVVALLSGGFDSPVAAYRLMKRGCFVTFVHFHAYPYVRPISIEKAVELARHLS------- 234

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                 +   + L    I            P+  V     L L +A   A++  A A++T
Sbjct: 235 ----RFQPPKNRLIVVPIGDAQRVIALAAEPSLRVVLYRRLMLRIATAIAHEEGAGALVT 290

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           G      +          + L +  ++G   D+    PL+  +K E  A A   G 
Sbjct: 291 GESLGQVAS---------QTLENMRAIGAVTDLPLLRPLVGNNKDEIIAEAVRIGT 337


>UniRef50_B7PXE7 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
           putative n=1 Tax=Ixodes scapularis RepID=B7PXE7_IXOSC
          Length = 384

 Score = 92.8 bits (229), Expect = 9e-18,   Method: Composition-based stats.
 Identities = 38/196 (19%), Positives = 61/196 (31%), Gaps = 25/196 (12%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR----AEIDV----ARELALK 52
            ++ V   SGG DS+       +   +V  V       H      ++D     A+ +   
Sbjct: 5   FRKVVCGMSGGVDSSVAAFLLRKSGYDVTGVFMRNWDPHDADSHCDLDADETDAKWVCDT 64

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
           LG     V  V        S +          E +    PN  +    ++       +A 
Sbjct: 65  LGIPFMTVDFVKPYWHNVFSPML--------QEYQDGLTPNPDIMCNRVIKFGAFHRHAV 116

Query: 113 QV-KAEAVITGVCETDFSGYPDCRDEFVKALNHAVSL-------GMAKDIRFETPLMWID 164
           +   A+AV TG         PD   E ++ ++            G A   R   PL    
Sbjct: 117 KRFGADAVATG-HYARIRNLPDGSVELLQGVDPVKDQSFFLSQVGQAALQRTLFPLGEAT 175

Query: 165 KAETWALADYYGKLDL 180
           K +  ALA   G   +
Sbjct: 176 KGQVKALARSLGLHRV 191


>UniRef50_Q8A2F1 tRNA-specific 2-thiouridylase mnmA 2 n=8 Tax=Bacteroides
           RepID=MNMA2_BACTN
          Length = 370

 Score = 92.8 bits (229), Expect = 9e-18,   Method: Composition-based stats.
 Identities = 45/186 (24%), Positives = 66/186 (35%), Gaps = 15/186 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAE-IDVARELALKLGARAHK 59
           KR ++  SGG DS+   ++ L+   EV  VTF  Y      E ++ AR LA +LG R H 
Sbjct: 6   KRVLLGMSGGTDSSVAAMRLLEAGYEVTGVTFRFYELNDSTEYLEDARHLAERLGIR-HI 64

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
             D   +         +  I    +E      P       N L   L +  A ++    +
Sbjct: 65  TYDAREIFR-------KQIIEYFVHEYMVGHTPVPCTLCNNYLKWPLLSKIADEMGIFYI 117

Query: 120 ITGVCETDFSGYPDCRDEFV---KALNHAVSLGMAKDI--RFETPLMWIDKAETWALADY 174
            TG           C   +             G+ +DI  R   P+  I K E  A A  
Sbjct: 118 ATGHYVRKVKVDDTCYITYAADSDKDQTFFLWGLKQDILRRMLLPMGDITKVEARAFAAE 177

Query: 175 YGKLDL 180
            G   +
Sbjct: 178 RGFQKV 183


>UniRef50_C5CE34 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Kosmotoga olearia TBF 19.5.1 RepID=C5CE34_KOSOT
          Length = 404

 Score = 92.8 bits (229), Expect = 9e-18,   Method: Composition-based stats.
 Identities = 37/186 (19%), Positives = 70/186 (37%), Gaps = 28/186 (15%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTF----DYGQRH-RAEIDVARELALKLGARAH 58
           A+++ SGG DS      AL++   +H + F      G++     +D+A+ L    G R  
Sbjct: 177 ALLLLSGGIDSPVAGWYALKRGITLHAIHFASPPYTGEKAFEKVLDLAKALTKYNGGRDM 236

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
            +  V           T+  + +     E+  +    V  R  +   +A   A +   +A
Sbjct: 237 LLYRVHF---------TKLQLAIHRNVRESLSL----VIQRRAMM-RIATKIAKEHGYKA 282

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           ++TG      +          + + +  ++G A DI    PL   DK ET  +A      
Sbjct: 283 IVTGENLGQVAS---------QTIENLHTIGSATDILVLRPLTGFDKIETIQIAKKIETF 333

Query: 179 DLVRNE 184
           ++    
Sbjct: 334 EISIQP 339


>UniRef50_C9KPU1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=2
           Tax=Clostridiales RepID=C9KPU1_9FIRM
          Length = 366

 Score = 92.4 bits (228), Expect = 9e-18,   Method: Composition-based stats.
 Identities = 40/193 (20%), Positives = 61/193 (31%), Gaps = 28/193 (14%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVT---------FDYGQRHRAEI---DVAREL 49
           K+ VV  SGG DS+       +Q  E   VT         FD   R    +   D AR +
Sbjct: 4   KKVVVAMSGGVDSSLTAALLKEQGYEAIGVTMRLSEESRDFDENDRGCCSLSSVDDARRV 63

Query: 50  ALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
           A  +G   H  ++   + +          I     +      PN  +     +       
Sbjct: 64  AEIIGI-PHYTMNFKEMFQ-------EKVIDYFLADYAKGWTPNPCIACNRYVKFEGLLQ 115

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALN-------HAVSLGMAKDIRFETPLMW 162
            A ++ A+ V TG      +     R    K ++           L       F  PL  
Sbjct: 116 KAMELGADYVATG-HYARIAQDESGRYVLKKGIDTHKDQSYALYHLNQQSLAHFLMPLGD 174

Query: 163 IDKAETWALADYY 175
             K ET  +A+ Y
Sbjct: 175 YTKVETREMAERY 187


>UniRef50_B6YWN1 ThiI-related protein n=5 Tax=Euryarchaeota RepID=B6YWN1_THEON
          Length = 196

 Score = 92.4 bits (228), Expect = 9e-18,   Method: Composition-based stats.
 Identities = 34/186 (18%), Positives = 64/186 (34%), Gaps = 20/186 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV + S G DS   +   L++  E+  V F   Q  + E     EL   L         
Sbjct: 2   KAVALLSSGIDSPVAIYLMLRKGMEITPVHFK--QSDKKESK-VLELCEILSKYGKLNEP 58

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V +      + +    + +   +        T         L  A    +++ A+A++TG
Sbjct: 59  VIVDAYEEQAPVFSKLVEIGKAKWTCLFCKYT--------MLRKACRVGHEIGAKAIVTG 110

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L++ + +  A D+    PL+  DK E   +A   G  ++  
Sbjct: 111 DSLGQVAS---------QTLDNLLIISQASDLPILRPLIGRDKEEIVKIAKEIGTFEISI 161

Query: 183 NETLTC 188
                C
Sbjct: 162 EPEEPC 167


>UniRef50_C7P7P9 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Methanocaldococcus fervens AG86 RepID=C7P7P9_METFA
          Length = 373

 Score = 92.4 bits (228), Expect = 1e-17,   Method: Composition-based stats.
 Identities = 26/194 (13%), Positives = 59/194 (30%), Gaps = 21/194 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           R + + S G DS       +++      +     +     ++ AR +A  L     ++  
Sbjct: 182 RVLCLVSDGIDSPVAAFMMIRRGCRDVLLHL---KMSEEGLNKARRIAEVLSDYDTELEF 238

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V       +  +      +                      L +A  YA  +  +A++TG
Sbjct: 239 VVYDYTNDIKDIVEKLKSIKKENYTCIFCKRR--------MLKVAEKYAKYLDCDAIVTG 290

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L +   +    +     PL+ +DK +   +A   G  ++  
Sbjct: 291 DNLGQVAS---------QTLKNLRVISEGINYPILRPLIGLDKNDIVEIAKEIGTYEIST 341

Query: 183 NETLTC-YNGFKGD 195
            + + C Y      
Sbjct: 342 EKEIKCPYLPKHPK 355


>UniRef50_Q5P5F5 Putative uncharacterized protein n=1 Tax=Aromatoleum aromaticum
           EbN1 RepID=Q5P5F5_AZOSE
          Length = 457

 Score = 92.4 bits (228), Expect = 1e-17,   Method: Composition-based stats.
 Identities = 41/246 (16%), Positives = 75/246 (30%), Gaps = 47/246 (19%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A++ +S G DS         +                      R + +++G++ H V   
Sbjct: 141 AIIAYSDGMDSRAVAGLESSRLGR-------------------RLVRMRVGSKQHDVSKK 181

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT-- 121
             L  +  ++L          + E           R   F  ++ + AY + A +VI   
Sbjct: 182 ERLR-IPFTALPYS------VKLENGRNAENSARSRGFKFSVVSTMAAYLIDAPSVIVPE 234

Query: 122 ---GVCE------TDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
              G                     F   +    +  +   I++  P +W+ K ET    
Sbjct: 235 SGQGALAPVLLPVGQGYEDYRNHPVFTALMARFFNALLGYKIQYRFPRLWMTKGETLREF 294

Query: 173 DYYGKLDLVRNETLTCYN-------GFKGDGCGHCAACNLRANGLNHYL---ADKPTVMA 222
                 +     T +C+          +   CG CAAC LR   ++        K  V  
Sbjct: 295 VDVCGSEARWATTRSCWQQSRQVAVSGERRQCGVCAACILRRLSVHAAGLNELPKTYVWE 354

Query: 223 AMKQKT 228
           ++K  T
Sbjct: 355 SLKAAT 360


>UniRef50_O67015 Putative uncharacterized protein n=1 Tax=Aquifex aeolicus
           RepID=O67015_AQUAE
          Length = 110

 Score = 92.4 bits (228), Expect = 1e-17,   Method: Composition-based stats.
 Identities = 29/113 (25%), Positives = 44/113 (38%), Gaps = 6/113 (5%)

Query: 98  GRNILFLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFE 157
            RN +  T  A+Y           G        + D + EF + L+  +S+ + K+   E
Sbjct: 1   MRNFILCTAGALYGVPKGINTCAIG--SLGMYPFEDNKKEFFEKLSETISMSLGKEFYIE 58

Query: 158 TPLMWIDKAETWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGL 210
           TP M + K E       +    +    +LTC N   G+ CG C  C  R   L
Sbjct: 59  TPFMGMHKHEVIKRYGKF----IPLELSLTCINPVNGEPCGKCIKCKEREEAL 107


>UniRef50_C4DG87 Predicted PP-loop superfamily ATPase n=1 Tax=Stackebrandtia
           nassauensis DSM 44728 RepID=C4DG87_9ACTO
          Length = 420

 Score = 92.0 bits (227), Expect = 1e-17,   Method: Composition-based stats.
 Identities = 44/225 (19%), Positives = 63/225 (28%), Gaps = 18/225 (8%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AV +FSGG DS             V      +G       +  REL   LG     V+  
Sbjct: 146 AVALFSGGLDSFCHAGLLRPASKVVL---VSHGLGPNL-RERKRELRAALGGE--TVMSA 199

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
                +     T    P P             V   N +      ++  +    A+    
Sbjct: 200 EFQVRINAVPGTERE-PHPRTRALLLLAAGVLVCAANGV----DTLHCPENGLLALHPAK 254

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRN 183
               +S            L +A+   +  D R   P   + K E    A   G    +  
Sbjct: 255 APL-WSQSATPIPPMTVHLANALLDSLDVDTRVVNPYAELTKGEVCERAVDAGWRRSLLG 313

Query: 184 ETLTCYNGFKGD-----GCGHCAACNLRANGLNHYLADKPTVMAA 223
            T+TC N  K        CG+C  C +R   L        T    
Sbjct: 314 RTITCVNPDKKSSGRFGNCGYCRHCLVRHAALVRVGG-DLTEYRD 357


>UniRef50_A7I9U0 Thiamine biosynthesis protein n=2 Tax=Genera incertae sedis
           RepID=A7I9U0_METB6
          Length = 213

 Score = 91.7 bits (226), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 30/181 (16%), Positives = 51/181 (28%), Gaps = 21/181 (11%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDY-GQRHRAEIDVARELALKLGARAHK 59
           M + V + SGG DS       L Q  E+  V  D         +  A  L   L    H 
Sbjct: 1   MAKLVSLISGGIDSPVAAYLMLHQGVEIIAVHMDNRPFTDEKNLHKAMNLIRHL---QHL 57

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
                    +             +                  +   +A   A +  A  +
Sbjct: 58  TQRPIKTYVVPHGPNHIAFARNCERHLHCLFCRR--------MMYRIAERIAEKEGAVGI 109

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           +TG      +          + L + +   +   I    PL+ +DK E   +A   G  +
Sbjct: 110 LTGESLGQVAS---------QTLQNLMVENLVIRIPVVRPLIGMDKVEIIDIARRIGTYE 160

Query: 180 L 180
           +
Sbjct: 161 I 161


>UniRef50_UPI0001BC5A6B tRNA (5-methylaminomethyl-2-thiouridylate) -methyltransferase n=2
           Tax=Fusobacterium RepID=UPI0001BC5A6B
          Length = 345

 Score = 91.7 bits (226), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 34/188 (18%), Positives = 57/188 (30%), Gaps = 18/188 (9%)

Query: 1   MK-RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH--RAEIDVARELALKLGARA 57
           MK + ++  SGG DS        ++  EV  V F        + E+   +++A K     
Sbjct: 1   MKEKVILGMSGGVDSAVAAYLLQKEGYEVIAVHFSVHNTEATQKELQDCQKIADKFSLTL 60

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           +         E  +S    ++       P      +        +   L    A + KA 
Sbjct: 61  YSYSLEKEFREKIISYYLSETGKGKTPSPCPLCDDS--------IKFHLLFQEAEKRKAN 112

Query: 118 AVITGVCET-------DFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
            V TG   +       + +                  L   K  +   PL  ++KAE   
Sbjct: 113 YVATGHYASISRNNICNTTLLEVKHHIHKDQCYMLYRLSPKKLNKILFPLASLEKAEVRE 172

Query: 171 LADYYGKL 178
           LA   G  
Sbjct: 173 LARDIGLF 180


>UniRef50_B1L665 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Candidatus Korarchaeum cryptofilum OPF8
           RepID=B1L665_KORCO
          Length = 373

 Score = 91.7 bits (226), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 29/186 (15%), Positives = 65/186 (34%), Gaps = 23/186 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + + + SGG DS       +++  ++  + F    +  AE +   ++   L   ++ V  
Sbjct: 176 KVLALISGGIDSPVAAWLLMRRGAQIIPLHFR---KSSAEEETFIKIVNVLRRYSYGVKM 232

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
             ++ E                E   +    T +  +  + LT A   A ++ A  ++TG
Sbjct: 233 EPMILEHGE----------FLSEISREAREWTCLLCKRRMLLT-ANRIAKEIGAHGIVTG 281

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L +        +     PL+ +DK +   +A   G  D+  
Sbjct: 282 DSLGQVAS---------QTLANLEVESRCLEKPVFRPLIGMDKEDIVKIAKEIGTYDISI 332

Query: 183 NETLTC 188
           +   +C
Sbjct: 333 SYKESC 338


>UniRef50_B7J5X6 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Acidithiobacillus
           RepID=MNMA_ACIF2
          Length = 352

 Score = 91.7 bits (226), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 39/187 (20%), Positives = 64/187 (34%), Gaps = 19/187 (10%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA----EIDVARELALKLGARA 57
           K+A++  SGG DS    +    Q  E+  VT     R R     +I+ A E+  +LG   
Sbjct: 4   KKALIALSGGVDSAVAALLMQGQGYELTGVTMRLWPRSRCCDEKDIEDAAEICARLGI-P 62

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           + VLD          +  R  + V   E +A   PN        L          ++ A 
Sbjct: 63  YTVLDYRE-------AFRRQVVDVFVAEYQAGRTPNPCARCNQFLKFDALLAEGEKLGAT 115

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNH------AVSLGMAKDIRFETPLMWIDKAETWAL 171
            + TG      +             +         ++G     R   P+  ++K E  A+
Sbjct: 116 MLATG-HYARLAETSSGTALLRGRDHAKDQSYFLFAIGAGILSRLRFPVGGMNKDEVRAM 174

Query: 172 ADYYGKL 178
           A  +G  
Sbjct: 175 ARKHGLP 181


>UniRef50_A8DJE2 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Candidatus Chloracidobacterium thermophilum
           RepID=A8DJE2_9BACT
          Length = 392

 Score = 91.3 bits (225), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 42/208 (20%), Positives = 62/208 (29%), Gaps = 32/208 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDV---------------- 45
           +R  V  SGG DS+T      +Q  +V   +     + R  +D                 
Sbjct: 3   ERIAVAMSGGVDSSTAAALLKEQGYDVVGFSMQLWNQRRINVDAEGNPLPSRCCSLDDLY 62

Query: 46  -ARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFL 104
            AR +A  LG   + +          V                    P+  V   + +  
Sbjct: 63  DARAVAEALGIPFYVLNFEEEFEARVVRPFVVS--------YLNGNTPSPCVACNSRMKF 114

Query: 105 TLAAIYAYQVKAEAVITGVCE----TDFSGYPDCRDEFVKALNH---AVSLGMAKDIRFE 157
                 A  V A  V TG        D +G  + R       +       L   +     
Sbjct: 115 DTLVALARDVGAAKVATGHYARVQFNDTTGRWELRKGRDLHKDQSYFLFELTQEQLAWAL 174

Query: 158 TPLMWIDKAETWALADYYGKLDLVRNET 185
            PL  + KAET ALA  YG     + E+
Sbjct: 175 FPLGELSKAETRALARRYGLPTAEKAES 202


>UniRef50_A6LSF2 tRNA-specific 2-thiouridylase mnmA n=19 Tax=Clostridiales
           RepID=MNMA_CLOB8
          Length = 358

 Score = 91.3 bits (225), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 32/195 (16%), Positives = 56/195 (28%), Gaps = 29/195 (14%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE------------IDVAREL 49
           K+ +V  SGG DS+       QQ  +V   T    Q                 +D AR +
Sbjct: 3   KKVLVGMSGGVDSSVAAYLLKQQGYDVIGATMQIWQHDEEFEEREGGCCSLSAVDDARRV 62

Query: 50  ALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
             KL    + +       E  +    ++ I      P  +   +        L       
Sbjct: 63  CDKLDIPFYVLNFRDYFKEKVIDKFVQEYIDGKTPNPCIECNKH--------LKFDELLR 114

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALN-------HAVSLGMAKDIRFETPLMW 162
            A  + A+ V TG          D R   +++ +          +    +      P   
Sbjct: 115 RARGIGADYVATGHYAK--IEKRDDRYLLIRSDDDRKDQTYALYNFTQDQLEHTLMPCGD 172

Query: 163 IDKAETWALADYYGK 177
            +K +   +A   G 
Sbjct: 173 YEKTKIREIAKEIGL 187


>UniRef50_B4R9S2 Putative uncharacterized protein n=1 Tax=Phenylobacterium zucineum
           HLK1 RepID=B4R9S2_PHEZH
          Length = 456

 Score = 91.3 bits (225), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 39/233 (16%), Positives = 71/233 (30%), Gaps = 20/233 (8%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDY-GQRHRAEIDVARELALKLGAR--AHKV 60
           +V +FSGG DS    +  L        V+  + G     +   A  +A +   +   H  
Sbjct: 151 SVCLFSGGLDSFIGAIDLLADGKAPMLVSHYWDGITSTHQTYCADVIAKRFPKQRFHHLR 210

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
             V   N+L   S   +++    +   A         G  ++ +    + +  V  +   
Sbjct: 211 ARVGFPNDLVDGSAGENTLRGRSFLFFALAAMAADALGVGLIHVPENGLISLNVPLDPTR 270

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
            G   T           ++   +  +  G+   IR E    +  K            L+ 
Sbjct: 271 LGALST-----RTTHPFYMARFDEILD-GLGLPIRLENRYRFQTKGVMAKNCADAALLNK 324

Query: 181 VRNETLTCYNGFKG-----------DGCGHCAACNLRANGLNHYLADKPTVMA 222
               T++C +  K              CGHC  C +R   +   L    T   
Sbjct: 325 EARHTMSCSSPAKARWAKDEDARQPKHCGHCVPCLIRRAAMVEGLGADHTPYQ 377


>UniRef50_O27720 Probable tRNA sulfurtransferase n=5 Tax=Methanobacteriaceae
           RepID=THII_METTH
          Length = 389

 Score = 91.3 bits (225), Expect = 2e-17,   Method: Composition-based stats.
 Identities = 34/179 (18%), Positives = 64/179 (35%), Gaps = 20/179 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEI-DVARELALKLGARAHKVL 61
           + V + SGG DS       +++  +V  V  D       E  +   ++A KL        
Sbjct: 179 KVVALLSGGIDSPVATYLMMKRGCQVVAVHMDNAPFTGEEAEEKVEKIAAKL-------A 231

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           + +   E  + + +             +    T V  +      LA + A +  A A++ 
Sbjct: 232 EYSAGVEFKLRTFSYGRYLESCRRGAPE--KMTCVLCK-FGMYHLAEMVAEEEGALAIVD 288

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           G      +          + L + ++  M  +I   +PL+ +DK E   LA   G  D+
Sbjct: 289 GSSLGQVAS---------QTLPNILATRMGVNIPILSPLIGMDKVEIENLAKRIGTYDI 338


>UniRef50_B0K991 tRNA-specific 2-thiouridylase mnmA 2 n=9 Tax=Clostridia
           RepID=MNMA2_THEP3
          Length = 364

 Score = 91.3 bits (225), Expect = 3e-17,   Method: Composition-based stats.
 Identities = 34/193 (17%), Positives = 55/193 (28%), Gaps = 28/193 (14%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR-------------AEIDVAREL 49
           R  +  SGG DS+       +Q  +V  +T      H                   A+++
Sbjct: 6   RVAIGMSGGVDSSVAAYLLKKQGFDVIGLTMRVWVDHEAKAFDSDKSCCSLKATQDAKKV 65

Query: 50  ALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
           A  LG   + V    +  +  V+            E      PN  V     +       
Sbjct: 66  AEILGIPHYTVDLSKIFYDKIVNYFV--------NEYLKGRTPNPCVLCNRQIKFGELLE 117

Query: 110 YAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMW 162
            A+++ A  + TG            R    K ++           L   +      PL  
Sbjct: 118 KAFELGAYYIATGHYVRKEYDEKTKRYLLKKGIDSSKDQSYVLYRLTQKQLEHALFPLGN 177

Query: 163 IDKAETWALADYY 175
             K E  ALA+  
Sbjct: 178 YKKEEIRALAEEL 190


>UniRef50_A5FHA9 tRNA-specific 2-thiouridylase mnmA n=26 Tax=Bacteria
           RepID=MNMA_FLAJ1
          Length = 395

 Score = 90.9 bits (224), Expect = 3e-17,   Method: Composition-based stats.
 Identities = 40/204 (19%), Positives = 60/204 (29%), Gaps = 27/204 (13%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEI----------DVARELA 50
           MKR VV  SGG DS+       QQ  EV  +           I          + A  +A
Sbjct: 1   MKRVVVGLSGGVDSSVAAYLLQQQGYEVIGLFMKNWHDDSVTISNECPWLEDSNDALLVA 60

Query: 51  LKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIY 110
            KLG     V       E  V  +          E E    PN  V     +   +    
Sbjct: 61  EKLGIPFQTVDLSEEYKEKIVDYMF--------NEYEKGRTPNPDVLCNREIKFDVFMKI 112

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEF-------VKALNHAV--SLGMAKDIRFETPLM 161
           A  + A+ V TG          D +  +       V          L   +  +   P+ 
Sbjct: 113 ALSLGADYVATGHYCQKSEIEVDGKTVYQLIAGNDVNKDQSYFLCQLSQEQLSKALFPIG 172

Query: 162 WIDKAETWALADYYGKLDLVRNET 185
            + K E   +A     +   + ++
Sbjct: 173 ALTKPEVREIAAEMELVTAEKKDS 196


>UniRef50_Q73PV6 tRNA-specific 2-thiouridylase mnmA n=2 Tax=Treponema
           RepID=MNMA_TREDE
          Length = 366

 Score = 90.9 bits (224), Expect = 3e-17,   Method: Composition-based stats.
 Identities = 36/199 (18%), Positives = 68/199 (34%), Gaps = 24/199 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR-------HRAEIDVARELALKLGA 55
           + +V  SGG DS       + Q  +V  VT     +          +I+ A+++A KLG 
Sbjct: 2   KVLVGLSGGVDSAVAAKLLIDQGYDVTGVTMQLLPKLSGIYKEQTDDIEDAKKVADKLGI 61

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
           + H V D+    +        + I     E +    PN      + +   L    A +  
Sbjct: 62  K-HIVYDMRETFK-------TEIIDYFVEEYKQGRTPNPCFICNSKIKFGLFLEQALKDG 113

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNH---------AVSLGMAKDIRFETPLMWIDKA 166
            + + TG          +  + F+                 L   +  R   PL    K 
Sbjct: 114 FDKIATGHYAKIEKTEIEGDERFLLRQAEDAQKDQSYFLALLTQEQLSRSIFPLGDFTKE 173

Query: 167 ETWALADYYGKLDLVRNET 185
           +  ++A+  G ++  R ++
Sbjct: 174 KVRSIAEDAGLINAHRPDS 192


>UniRef50_A7ZEK1 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Campylobacter
           RepID=MNMA_CAMC1
          Length = 340

 Score = 90.9 bits (224), Expect = 3e-17,   Method: Composition-based stats.
 Identities = 34/189 (17%), Positives = 59/189 (31%), Gaps = 12/189 (6%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARAHK 59
           + +V  SGG DST       +   EV        Q+   H   I   +++   LG + H 
Sbjct: 2   KIMVAMSGGVDSTMTAKFLQEAGHEVQGCYMMLHQKPGYHEENIRKVKKVGEYLGIKVHI 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +      NE       R         P A             + L     +A     E +
Sbjct: 62  LDLQDKFNEYVYDPFVRLYKEGKTPNPCALCNK--------FIKLGALLDFAKVNGCEKL 113

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR-FETPLMWIDKAETWALADYYGKL 178
            TG       G+  C  +  K  ++ ++    + ++    PL    K +   LA     L
Sbjct: 114 ATGHYVQVVDGFITCAKDPSKDQSYFLAQVPKEVLKDVIFPLGDKFKKDIKELARGVKVL 173

Query: 179 DLVRNETLT 187
           +    +  +
Sbjct: 174 EEFATQAES 182


>UniRef50_A6W0E1 tRNA-specific 2-thiouridylase mnmA n=20 Tax=Bacteria
           RepID=MNMA_MARMS
          Length = 390

 Score = 90.5 bits (223), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 36/209 (17%), Positives = 76/209 (36%), Gaps = 26/209 (12%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR--------HRAEIDVARELALKL 53
           K+ +V  SGG DS+   +  +QQ  +V  +                + ++  A+ ++ KL
Sbjct: 31  KKVIVGMSGGVDSSVSALILMQQGYQVEGLFMKNWDEDDGTEYCTAKDDLADAQAVSDKL 90

Query: 54  GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           G + H     +            +       E +A   PN  +     +       YA  
Sbjct: 91  GIKLHTANFASEYW--------DNVFEHFLAEYKAGRTPNPDILCNKEIKFKAFLEYAMA 142

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKA 166
           + A+ + TG       G  +     +K L+          ++G  +  +   P+  ++K 
Sbjct: 143 LNADYIATGHYV--RRGEKNGEPLLLKGLDGNKDQSYFLYAVGKDEIAKTLFPVGELEKT 200

Query: 167 ETWALADYYGKLDLVRNETLT-CYNGFKG 194
           E   LA+ +G +   + ++   C+ G + 
Sbjct: 201 EVRRLAEEFGLITHNKKDSTGICFIGERR 229


>UniRef50_UPI000197B781 hypothetical protein BACCOPRO_02286 n=1 Tax=Bacteroides coprophilus
           DSM 18228 RepID=UPI000197B781
          Length = 361

 Score = 90.5 bits (223), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 38/190 (20%), Positives = 57/190 (30%), Gaps = 25/190 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAE-IDVARELALKLGARAHKV 60
           + ++  SGG DS+   +       EV  +TF  Y +  + E ++ AR L  +LG R H  
Sbjct: 15  KVLLGMSGGTDSSVAAMLLQDAGYEVTGITFRFYEKDFQTEYLEDARHLCEQLGIR-HLT 73

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
            D   + E  +             E      P       N L   L    A +     + 
Sbjct: 74  YDAREVFEEQIIHYFIQ-------EYMNGRTPVPCTLCNNQLKWPLLKEIADREGIYHLA 126

Query: 121 TGVCE----------TDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
           TG                   PD    F         L      R   P+  + K     
Sbjct: 127 TGHYVRKQTVNGRWFIRSGADPDKDQSFF-----LWGLPQDLLERALFPMGELTKVRVRE 181

Query: 171 LADYYGKLDL 180
           +A   G+L  
Sbjct: 182 IAAERGQLKA 191


>UniRef50_Q2FLN6 Thiamine biosynthesis protein n=1 Tax=Methanospirillum hungatei
           JF-1 RepID=Q2FLN6_METHJ
          Length = 387

 Score = 90.5 bits (223), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 38/186 (20%), Positives = 66/186 (35%), Gaps = 19/186 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           +AV + S G DS       +++   +  +  + G+    +I   R L   L A +H V  
Sbjct: 172 KAVALISAGIDSPVAAWLMMRRGVRMIFLHIEPGKFGGEDIR--RNLENNLAALSHWVPG 229

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           + L        +T  + P               V  +    L LA+ YA +   + +ITG
Sbjct: 230 LELPV------ITIPAEPFYQALMTLSEPKLRCVLCKRG-MLELASRYAQKSGCDGIITG 282

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L++   +    +I    PL+  DK E   LA   G      
Sbjct: 283 DNLGQVA---------TQTLHNLAPISAGIEIPIYRPLIGYDKEEIITLARKIGSFSSEP 333

Query: 183 NETLTC 188
            +T +C
Sbjct: 334 GDT-SC 338


>UniRef50_C7M0G9 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase n=1
           Tax=Acidimicrobium ferrooxidans DSM 10331
           RepID=C7M0G9_ACIFD
          Length = 341

 Score = 90.5 bits (223), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 41/189 (21%), Positives = 63/189 (33%), Gaps = 19/189 (10%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-------DYGQRHRAEIDVARELALKL 53
           M R VV  SGG DS+   + A +   +V  VT        D G     +++ AR +A  L
Sbjct: 1   MARVVVAMSGGVDSSVAALMAREAGHDVIGVTLRLTEAAGDRGCCSVGDVEDARTVAALL 60

Query: 54  GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           G            +   V                    PN  +     + +T  A +A +
Sbjct: 61  GINHIVADMRAEFHRAVVEPYLDAH--------RRGWTPNPCMSCNRYVKVTALAAWAQR 112

Query: 114 VKAEAVITGVCET--DFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETW 169
           + A  ++TG   T  D S     R    +     V   +  ++  R   PL  + K E  
Sbjct: 113 LGASRLVTGHYATVLDGSRPMLVRGRDRRKDQSYVLADVPPEVLARLWLPLGELSKDEVR 172

Query: 170 ALADYYGKL 178
             A   G  
Sbjct: 173 TRAHAAGLP 181


>UniRef50_B9QRC5 Putative uncharacterized protein n=1 Tax=Labrenzia alexandrii
           DFL-11 RepID=B9QRC5_9RHOB
          Length = 457

 Score = 90.5 bits (223), Expect = 4e-17,   Method: Composition-based stats.
 Identities = 42/231 (18%), Positives = 67/231 (29%), Gaps = 46/231 (19%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           A++ FS G DS                     G     ++   R  +  +G         
Sbjct: 144 AIMAFSDGMDSRAVAGLI--------------GSGLGPKLVRVRVGSNDIGRPD------ 183

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT-- 121
               +      T     VP   P  +    +    R   F  ++ I AY   A  +I   
Sbjct: 184 ---KKNRREPFTMVPYDVPCNMPNREASARS----RGFKFALISGIAAYLSGASEIIIPE 236

Query: 122 -GVCETDFSGYPD--------CRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
            G      +  P             F + +   +     K+IR+  P +W  K ET   +
Sbjct: 237 SGQGAIGPAIAPVVHAYPDYRNHPRFTQRMERLLKALFGKEIRYVFPRLWNTKGETLR-S 295

Query: 173 DYYGKLDLVRNETLTCYN-------GFKGDGCGHCAACNLRANGLNHYLAD 216
                     + T +C+          K   CG CAAC LR   ++     
Sbjct: 296 FATLPEGSNWHTTRSCWRSNRWCSVNGKRRQCGVCAACMLRRLSVHAAGLT 346


>UniRef50_A1STI9 tRNA-specific 2-thiouridylase mnmA n=4 Tax=Proteobacteria
           RepID=MNMA_PSYIN
          Length = 367

 Score = 90.1 bits (222), Expect = 5e-17,   Method: Composition-based stats.
 Identities = 30/208 (14%), Positives = 69/208 (33%), Gaps = 25/208 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR--------HRAEIDVARELALKLG 54
           + +V  SGG DS+       QQ   V  +     +            +++ A+ +  KLG
Sbjct: 8   KVIVGMSGGVDSSVSAYLLQQQGYLVEGLFMKNWEEDDTDQYCAAAQDLEDAQSVCDKLG 67

Query: 55  ARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
              H +       +        +        P+        +  + I F       A  +
Sbjct: 68  IPLHTINFAAEYWDNVFEHFLAEYKAGRTPNPD-------IMCNKEIKFKAFLEFAAEDL 120

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKAE 167
            A+ + TG          D   + ++ L++         ++G  +  +   P+  ++K E
Sbjct: 121 GADYIATGHYV--RRREVDGHVQLLRGLDNNKDQSYFLYAIGEKQIAKSLFPVGELEKPE 178

Query: 168 TWALADYYGKLDLVRNETLT-CYNGFKG 194
              +A+    +   + ++   C+ G + 
Sbjct: 179 VRRIAEQQDLITHNKKDSTGICFIGERK 206


>UniRef50_Q58341 Probable tRNA sulfurtransferase n=11 Tax=Methanococcales
           RepID=THII_METJA
          Length = 381

 Score = 90.1 bits (222), Expect = 5e-17,   Method: Composition-based stats.
 Identities = 23/194 (11%), Positives = 59/194 (30%), Gaps = 21/194 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + + + S G DS       +++      +     +     ++  R++   L     ++  
Sbjct: 190 KVLCLISDGIDSPVAAFMMIRRGCRAVLLHL---KMSEEALNKVRKIVEVLSDYDTELEF 246

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
           V    +  +  +      +                      L +A  YA  +  +A++TG
Sbjct: 247 VVYDYKKDIEDIVEKLKSIKKENYTCIFCKR--------KMLKVAEKYAKYLDCDAIVTG 298

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          + L +   +    +     PL+ +DK +   +A   G  ++  
Sbjct: 299 DNLGQVAS---------QTLKNLRVISENINYPILRPLIGLDKNDIVKIAKEIGTYEIST 349

Query: 183 NETLTC-YNGFKGD 195
            + + C Y      
Sbjct: 350 EKEIKCPYLPKHPK 363


>UniRef50_Q3DBK5 TRNA methyl transferase (Fragment) n=5 Tax=cellular organisms
           RepID=Q3DBK5_STRAG
          Length = 204

 Score = 90.1 bits (222), Expect = 5e-17,   Method: Composition-based stats.
 Identities = 32/199 (16%), Positives = 59/199 (29%), Gaps = 24/199 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR--------HRAEIDVARELALKLG 54
           R VV  SGG DS+   +   +Q  +V  V                  +      +A ++G
Sbjct: 8   RVVVGMSGGVDSSVTALLLKEQGYDVIGVFMKNWDDTDEFGVCTATEDYKDVAAVADQIG 67

Query: 55  ARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQV 114
              + V                        E  A   PN  V     +       YA  +
Sbjct: 68  IPYYSVNFEKEYW--------DRVFEYFLAEYRAGRTPNPDVMCNKEIKFKAFLDYAMTL 119

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKAE 167
            A+ V TG      +   +     ++ +++          L   +  +   PL  + K E
Sbjct: 120 GADYVATG-HYAQVTRDKNGIVHMLRGVDNNKDQTYFLSQLSQEQLQKTLFPLGHLQKPE 178

Query: 168 TWALADYYGKLDLVRNETL 186
              +A+  G     + ++ 
Sbjct: 179 VRRIAEEAGLATAKKKDST 197


>UniRef50_C9RAV2 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Ammonifex degensii KC4 RepID=C9RAV2_AMMDK
          Length = 344

 Score = 90.1 bits (222), Expect = 6e-17,   Method: Composition-based stats.
 Identities = 42/185 (22%), Positives = 54/185 (29%), Gaps = 18/185 (9%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M R  V  SGG DS T     LQ   EV  VT         E + A E+A KL       
Sbjct: 1   MTRVAVALSGGVDSATAAFLLLQAGYEVFGVTM---LVTGEEGEKAAEVAAKLKIPHFVF 57

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                  +  +                A   PN  V     L   L    A ++ A+ + 
Sbjct: 58  DLRQEFAQEVIKPFVEA--------YLAGLTPNPCVWCNRKLKFGLLLEKALKLGADFLA 109

Query: 121 TGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKAETWALAD 173
           TG     +      R    +  +          SL   +  R   PL    KAE   L  
Sbjct: 110 TGHYARVWFDPARERYLLARGKDRRKDQSYFLYSLNQQQLARVLFPLGGRTKAENLLLVK 169

Query: 174 YYGKL 178
             G  
Sbjct: 170 KLGLP 174


>UniRef50_A5UV64 tRNA-specific 2-thiouridylase mnmA n=7 Tax=Bacteria
           RepID=MNMA_ROSS1
          Length = 373

 Score = 89.7 bits (221), Expect = 6e-17,   Method: Composition-based stats.
 Identities = 38/219 (17%), Positives = 63/219 (28%), Gaps = 42/219 (19%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ-----------RHRAEIDVAREL 49
           M + +V  SGG DS+       +   +V  VT    +             +   + AR +
Sbjct: 1   MAKIMVAMSGGVDSSLAAALLHEAGHDVTGVTLHLWEGDDDRLAESLCCSQEMTESARRV 60

Query: 50  ALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAI 109
             +LG   +            +    R        E  +   PN  +     +       
Sbjct: 61  CAQLGIPYYVFNYQREFRRYVIEYFLR--------EYASGFTPNPCLECNREIKFRALLA 112

Query: 110 YAYQVKAEAVITG-----------VCETDFSGYPDCRDE-----FVKALNH-------AV 146
            A  +  + V TG               D    P  R +      ++A++          
Sbjct: 113 RARTLGFDYVATGHYARIRADETRHNGEDAGLPPAERAQRTTYRLLRAVDREKDQSYMLY 172

Query: 147 SLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNET 185
            LG     R   P+    KAE  A+A   G     R E+
Sbjct: 173 MLGQDDLARLIFPIGEYTKAEVRAMAAARGLASANRPES 211


>UniRef50_Q2LWA6 tRNA-specific 2-thiouridylase mnmA 1 n=1 Tax=Syntrophus
           aciditrophicus SB RepID=MNMA1_SYNAS
          Length = 372

 Score = 89.7 bits (221), Expect = 6e-17,   Method: Composition-based stats.
 Identities = 41/194 (21%), Positives = 57/194 (29%), Gaps = 28/194 (14%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH---------RAEIDVARELALK 52
           KR ++  SGG DS+   +   ++  EV  VT   G R          R  I+ AR +   
Sbjct: 21  KRVLLAISGGVDSSVAALLLKEEGYEVAGVTMCLGVREEENKVRCCGREAIEDARGVCEI 80

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
           LG   H VLD   L       L    I     E      PN  +     L        A 
Sbjct: 81  LGI-PHYVLDYAPL-------LETCVIDKFVREYRLGRTPNPCIDCNRYLKFGHLLDSAR 132

Query: 113 QVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVS--------LGMAKDIRFETPLMWID 164
            +  + + TG                +K     V         + +A       PL    
Sbjct: 133 TMGFDYLATGHYA---KIERKESRWILKKAKDLVKDQTYFLYPIPVAALEHILFPLADRT 189

Query: 165 KAETWALADYYGKL 178
           K E   +A      
Sbjct: 190 KDEVREIARQALLP 203


>UniRef50_B5CVE8 Putative uncharacterized protein n=1 Tax=Bacteroides plebeius DSM
           17135 RepID=B5CVE8_9BACE
          Length = 233

 Score = 89.7 bits (221), Expect = 7e-17,   Method: Composition-based stats.
 Identities = 32/224 (14%), Positives = 65/224 (29%), Gaps = 49/224 (21%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHC-VTFDYGQRHRAEIDVARELALKLGARAHKV 60
           ++ V+++SGG DS       + +  +    +  D G ++  E                ++
Sbjct: 7   EKKVLLYSGGMDS-----WLIDKIWKPDVKLYVDMGTKYSQE----------------EI 45

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
             +          +  + + +  +E E        +P RN+  + +A  Y        + 
Sbjct: 46  KRLPA-------DVVVEKLDLSKWEREDKI-----IPLRNMYLIGIATNYG-----NEIC 88

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSL-------GMAKDIRFETPLMWIDKAETWA-LA 172
            G    D     D    F       ++           + I+         KAE      
Sbjct: 89  LGATAGD--RVLDKSPVFADIYEQLLNYLYQKQHWTEERKIKINLDFKRYTKAELVRAFI 146

Query: 173 DYYGKLDLVRNETLTCYNGFKGDGCGHCAACNLRANGLNHYLAD 216
              G +D   + + +CY    G  C  C  C  +          
Sbjct: 147 AQGGDMDEAFSSSFSCYTPEHGKECWSCKPCFRKFIAFALNGYP 190


>UniRef50_C9L0F1 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Bacteroides finegoldii DSM 17565 RepID=C9L0F1_9BACE
          Length = 365

 Score = 89.7 bits (221), Expect = 8e-17,   Method: Composition-based stats.
 Identities = 43/191 (22%), Positives = 62/191 (32%), Gaps = 25/191 (13%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAE-IDVARELALKLGARAHK 59
           +R ++  SGG DS+   ++ L+   EV  +TF  Y      E ++ AR LA +LG R H 
Sbjct: 6   RRVLLGMSGGTDSSVAAMRLLEGGYEVTGITFRFYELNDSTEYLEDARSLAERLGIR-HI 64

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
             D   +         +  I     E      P       N L   L A  A ++    +
Sbjct: 65  TYDARDVFR-------KQIIEYFVQEYMEGRTPVPCTLCNNCLKWPLLAKVADEMGIFYI 117

Query: 120 IT----------GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
            T          G      +  PD    F         L      R   P+  I K E  
Sbjct: 118 ATGHYVQKVELEGTYYITHAADPDKDQSFF-----LWGLKQDILRRILLPMGNITKVEAR 172

Query: 170 ALADYYGKLDL 180
             A   G   +
Sbjct: 173 VWAAERGFQKV 183


>UniRef50_B2A8M8 Probable tRNA sulfurtransferase n=2 Tax=Clostridia RepID=THII_NATTJ
          Length = 413

 Score = 89.3 bits (220), Expect = 8e-17,   Method: Composition-based stats.
 Identities = 32/185 (17%), Positives = 66/185 (35%), Gaps = 25/185 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD----YGQRHRAEIDVARELALKLGARAH 58
           + V++ SGG DS      A+++  +V  + F       QR   +++   ++  + G    
Sbjct: 185 KGVLMLSGGIDSPVAGWLAMKRGIQVVGLHFHSYPFTSQRALKKVEDISQVLSRYGTGP- 243

Query: 59  KVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
                T   +L  +  T     + +Y        + +V         +A   A + +A  
Sbjct: 244 -----TGGFKLITNHFTDIQKAIQNY-----CSESMWVTVMRRFMFYIANRMAQKEQAMT 293

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMA-KDIRFETPLMWIDKAETWALADYYGK 177
           V+TG      +          + L    ++     ++    PL  +DK E  + A+  G 
Sbjct: 294 VVTGENVGQVAS---------QTLESMHAVSQDVVNLPILRPLAGLDKKEIMSKAETIGT 344

Query: 178 LDLVR 182
            D+  
Sbjct: 345 YDISI 349


>UniRef50_A6KZV1 tRNA-specific 2-thiouridylase mnmA 3 n=23 Tax=Bacteroides
           RepID=MNMA3_BACV8
          Length = 375

 Score = 89.3 bits (220), Expect = 9e-17,   Method: Composition-based stats.
 Identities = 40/195 (20%), Positives = 56/195 (28%), Gaps = 33/195 (16%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDY----------GQRHRAEIDVARELAL 51
           KR +V  SGG DS+   +   +Q  EV  VT             GQ    ++  AR LA 
Sbjct: 6   KRVLVGMSGGIDSSATCIMLQEQGYEVVGVTMRTWDVPSRFSTPGQDEPDDVLEARALAE 65

Query: 52  KLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           +LG   H         ++ V             E      PN  V    +    +   +A
Sbjct: 66  RLGIEHHVADVRKEFKQIIVKHFID--------EYMQGRTPNPCVLCNPLFKERILCEWA 117

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNH----------AVSLGMAKDIRFETPLM 161
            Q     + TG          + R   +  +               L      RF  PL 
Sbjct: 118 DQTNCAHISTG-----HYCRLEERKGKLYIVAGDDQTKDQSYFLWKLPQEILQRFLFPLG 172

Query: 162 WIDKAETWALADYYG 176
              K E        G
Sbjct: 173 TYTKQEVREYLRQKG 187


>UniRef50_A3CVZ1 ExsB family protein n=1 Tax=Methanoculleus marisnigri JR1
           RepID=A3CVZ1_METMJ
          Length = 257

 Score = 89.0 bits (219), Expect = 1e-16,   Method: Composition-based stats.
 Identities = 37/180 (20%), Positives = 61/180 (33%), Gaps = 33/180 (18%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
             V  SGG DS+  L  A     +V  ++ D G     E+  AR+L+ +LG   H V+ +
Sbjct: 17  VAVALSGGTDSSVLLALARHHGIQVIAISVDTGLTPPGELAAARKLSERLGL-PHVVIPL 75

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
            +L+                     +     +V  R ++   +A   A +     V+ G 
Sbjct: 76  DMLDI---------------PAVRENRPDRCYVCKRAMMEAIVAE--ARRQGCRTVVDGT 118

Query: 124 CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLM--WIDKAETWALADYYGKLDLV 181
              D       R      +     LG       ++P     + KA+  ALA   G     
Sbjct: 119 HADD-------RPAARPGMRVLSELG------IKSPFAECGMGKADVEALARELGVPVRP 165


>UniRef50_B4D711 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Chthoniobacter flavus Ellin428 RepID=B4D711_9BACT
          Length = 363

 Score = 88.6 bits (218), Expect = 1e-16,   Method: Composition-based stats.
 Identities = 43/205 (20%), Positives = 63/205 (30%), Gaps = 25/205 (12%)

Query: 8   FSGGQDSTTCLVQALQQYDEVHCVTF-----DYGQRHRAE------IDVARELALKLGAR 56
            SGG DS+       +Q  +V  VT      D   R   +      I  AR +A  LG  
Sbjct: 1   MSGGVDSSVAAYLLKRQGYDVVGVTMKVWPQDCISRAEDKCCGPQAIADARGVAHALGVP 60

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            + V +      L +   T         E +    PN  V     +        A  + A
Sbjct: 61  HYVVDEGENFERLVIDYFTE--------EYKNGRTPNPCVMCNEKVKFGNLWGKAKALGA 112

Query: 117 EAVITGV-----CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWAL 171
           E + TG         D +     RD          SL  A+      PL  + K E  A+
Sbjct: 113 EYIATGHYAIIEHHADRAVLRKGRDPRKDQSYFLFSLRQAQLQHAICPLGEMSKPEIRAI 172

Query: 172 ADYYGKLDLVRNETLT-CYNGFKGD 195
           A   G     + ++   C+      
Sbjct: 173 ARQLGLKVAEKEDSQEICFVPGNDY 197


>UniRef50_C7NES3 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase n=5
           Tax=Fusobacteriaceae RepID=C7NES3_LEPBD
          Length = 364

 Score = 88.2 bits (217), Expect = 2e-16,   Method: Composition-based stats.
 Identities = 44/215 (20%), Positives = 68/215 (31%), Gaps = 34/215 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-----------IDVARELA 50
           K+ VV  SGG DS+   +   QQ  EV  VT  +     +E           I+ AR   
Sbjct: 8   KKVVVGMSGGIDSSVAALLLKQQGYEVIGVTLKHLPDELSENPGKTCCSLDDINDARYTC 67

Query: 51  LKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIY 110
             LG   + +  V            +D +        A   P+  V     + +     +
Sbjct: 68  YNLGIPHYVLNVVEEFK--------KDVMEYFIKMYNAGKTPSPCVICDEKVKIKKLVEF 119

Query: 111 AYQVKAEAVITGV---CETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAE 167
           A ++  + + TG       +     D  +   +       L      RF  PL   +K+E
Sbjct: 120 ADKMGIKYISTGHYSKVSENNMLLWDKNNRKDQTY-MLYRLDKDIVERFLFPLAEYEKSE 178

Query: 168 TWALADYYGKLDLVRNETLTCYNGFKGDGCGHCAA 202
              +A   G        T       K D  G C A
Sbjct: 179 VREIARQNGI------HTH-----NKPDSQGICFA 202


>UniRef50_A8WT10 Putative uncharacterized protein n=2 Tax=Caenorhabditis briggsae
           RepID=A8WT10_CAEBR
          Length = 808

 Score = 88.2 bits (217), Expect = 2e-16,   Method: Composition-based stats.
 Identities = 37/190 (19%), Positives = 59/190 (31%), Gaps = 14/190 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+ +V+ SGG DS  C     +      V  V  D G     E D        L    H+
Sbjct: 338 KKVLVMVSGGVDSAVCAALLNRALGPGRVTAVHIDNGFMRHLESDAVEMSLKALDLPVHR 397

Query: 60  VLDVTLLNELAVSSLTRDS--IPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
               T     +   L  +     + D E +   I NTF+  ++++   L           
Sbjct: 398 YDYGTTFRSSSEHMLPEEKALDEIDDPELKRKIIGNTFIRVKDLIMNELK----INHNDY 453

Query: 118 AVITGVCETD----FSGYPDCRDEFVKAL--NHAVSLGMAKDIRFETPLMWIDKAETWAL 171
            +  G    D     S       + +K    +  +   + K  +   PL    K E   L
Sbjct: 454 FLAQGTLRPDLIESASELASGHADTIKTHHNDTYLVRELRKAGKVIEPLKDFHKDEVRVL 513

Query: 172 ADYYGKLDLV 181
               G  D +
Sbjct: 514 GKDLGLPDAI 523


>UniRef50_C7DGN0 Thiamine biosynthesis protein n=1 Tax=Candidatus Micrarchaeum
           acidiphilum ARMAN-2 RepID=C7DGN0_9EURY
          Length = 395

 Score = 88.2 bits (217), Expect = 2e-16,   Method: Composition-based stats.
 Identities = 35/187 (18%), Positives = 64/187 (34%), Gaps = 31/187 (16%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRH----RAEIDVARELALKLGARA 57
           RAVV+ SGG DS      A+++  +V  +    Y   H        ++  +LA   G   
Sbjct: 177 RAVVLLSGGIDSPVAAFYAMRRGFDVVYLHIHGYDDPHTAMASKMHELVGQLAKYSGKTR 236

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
                  +    A+ S  R  +                V  +  ++L L+   A ++  +
Sbjct: 237 VYYAPFHIFQMAAMGSAPRYEL----------------VLFKRFIYL-LSEKMADEMNID 279

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A++TG      +          + +++  S           PL+  DK E   +A   G 
Sbjct: 280 AIVTGESLGQVAS---------QTVSNLGSAQSGVRHLIFRPLIGFDKNEIIEMARRIGT 330

Query: 178 LDLVRNE 184
            D+    
Sbjct: 331 YDISIKP 337


>UniRef50_Q2RZN9 tRNA-specific 2-thiouridylase mnmA n=11 Tax=Bacteroidetes
           RepID=MNMA_SALRD
          Length = 383

 Score = 87.8 bits (216), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 39/199 (19%), Positives = 62/199 (31%), Gaps = 29/199 (14%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF---DY---GQRHRAEI--------DVARE 48
           R +V  SGG DS+   V   ++  +V  +T    DY   G R   E+        + AR 
Sbjct: 6   RVLVAMSGGVDSSVTAVLLKERGYDVVGLTMKTWDYSTSGGRDGKEVGCCSIESMNDARV 65

Query: 49  LALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
           +A + G     V       +  +   T         E  +   PN  V     +      
Sbjct: 66  VATEHGFPHFVVDLREEFGDWVIERFTD--------EYLSGRTPNPCVLCNTHIKWDALL 117

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLM 161
             A  +  E + TG            R    + L+           L      R   PL 
Sbjct: 118 QRADDLDCEYIATGHYANVRYDDERDRYLLSRGLDRNKDQSYALWGLPQEHLARSIFPLG 177

Query: 162 WIDKAETWALADYYGKLDL 180
             +K E   +A  +G  ++
Sbjct: 178 AYEKPEIREMAAEFGLDNV 196


>UniRef50_A3DEB6 Probable tRNA sulfurtransferase n=34 Tax=Bacteria RepID=THII_CLOTH
          Length = 392

 Score = 87.8 bits (216), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 35/192 (18%), Positives = 61/192 (31%), Gaps = 35/192 (18%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYGQRHRAEIDVARELALKL-----GAR 56
           +AV++ SGG DS        ++  E+  V F  Y        +   EL   L        
Sbjct: 181 KAVLLLSGGIDSPVAGWMIAKRGVEIEAVHFYSYPYTSERAKEKVIELTKILATYCQKIN 240

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H V              T   + + +  P  +      +  R  +   +A I A +  A
Sbjct: 241 LHIV------------PFTEIQLEINEKCPHEELT----IIMRRAMM-RIAEIIANKTGA 283

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A++TG      +          + +   V       +    PL+ +DK E   +A   G
Sbjct: 284 LALVTGESVGQVAS---------QTIQSLVVTNAVVSLPVFRPLIGMDKNEVVDIAKKIG 334

Query: 177 KLD---LVRNET 185
             +   L   + 
Sbjct: 335 TFETSILPYEDC 346


>UniRef50_Q3SW10 Putative uncharacterized protein n=1 Tax=Nitrobacter winogradskyi
           Nb-255 RepID=Q3SW10_NITWN
          Length = 452

 Score = 87.8 bits (216), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 45/238 (18%), Positives = 67/238 (28%), Gaps = 52/238 (21%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AV+ +S G DS                                   A  LG R  +V   
Sbjct: 144 AVIAYSDGMDSRAVAGLM----------------------------AESLGERLVRVRVG 175

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV---- 119
               +           P             T    R   F  ++ I AY  +A+ +    
Sbjct: 176 P---KAGGRPFKGQREPFAAVPYSVAATGETSGRSRGFKFALISGIAAYLAEADVILVPE 232

Query: 120 ----ITG-VCETDFSGYPDCR--DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
                 G    T    YPD R    F   +   ++    + +RF  P +W  KAET    
Sbjct: 233 SGQGAFGPALVTVAHAYPDYRNHPLFTSRMERFLNALFRRPVRFVFPRLWHTKAETLREY 292

Query: 173 DYYGKLDLVRNETLTCYNGFKG-------DGCGHCAACNLRANGLNHYLADK-PTVMA 222
                     ++T +C+   +          CG CAAC LR   ++       P    
Sbjct: 293 AAL--PGSKWDDTKSCWRNARWSALNGNLVQCGICAACMLRRMSIHTAGLVDGPDAYV 348


>UniRef50_UPI000069DE11 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC
           2.1.1.61) (Mitochondrial tRNA-specific 2-thiouridylase
           1) (MTO2 homolog). n=6 Tax=Euteleostomi
           RepID=UPI000069DE11
          Length = 372

 Score = 87.4 bits (215), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 34/217 (15%), Positives = 61/217 (28%), Gaps = 45/217 (20%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQ--------RHRAEIDVARELALK 52
           ++R V   SGG DS+   +   ++  EV  V  +               + + A  +  K
Sbjct: 4   VRRVVCAMSGGVDSSVAALLLRRRGYEVTGVFMNNWDAVDEHGVCSAERDCEDAYRVCKK 63

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA- 111
           L    H+V  V        S            E E    PN  +     +       YA 
Sbjct: 64  LDIPFHQVSYVKEYWHEVFSYFL--------NEYEKGRTPNPDILCNKHIKFNHFLSYAI 115

Query: 112 YQVKAEAVITGVCE-TDFSGYPDCRDEFVKALNHAVS----------------------- 147
             + A+A+ TG    T        + +++K+  +                          
Sbjct: 116 DILGADAIATGHYARTSLEDEEIFQQKYIKSPQNLFRNRFEIRHDAKLLQAADRIKDQTF 175

Query: 148 ----LGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
               +      +   PL  + K     +A   G   +
Sbjct: 176 FLSQISQYALRKTIFPLGELTKDFVKKIAAEAGFHHV 212


>UniRef50_D0LWB4 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase n=1
           Tax=Haliangium ochraceum DSM 14365 RepID=D0LWB4_HALO1
          Length = 391

 Score = 87.4 bits (215), Expect = 4e-16,   Method: Composition-based stats.
 Identities = 38/199 (19%), Positives = 57/199 (28%), Gaps = 25/199 (12%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTF----------DYGQR--HRAEIDVARELAL 51
            V   SGG DS       ++Q   V  +T             G R     +I+ AR +  
Sbjct: 7   IVAALSGGVDSAVTAAMLVEQGHRVVGMTMRLYDASGTSASVGGRCCGPRDIEDARRVCA 66

Query: 52  KLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
            LG   + V          +                A   PN  V     +  T     A
Sbjct: 67  HLGIPFYVVNFEVDFAHTVIDDFVDT--------YLAGETPNPCVKCNQHIKFTPLLARA 118

Query: 112 YQVKAEAVITGVCE--TDFSGYPDCRDEFVKALNH---AVSLGMAKDIRFETPLMWIDKA 166
             + AE V TG      D       R     + +      S+   +      PL  + K 
Sbjct: 119 RALGAETVATGHYARLEDTGAGIRLRRGRDASKDQSYFLFSMPREELASVRFPLGAMTKD 178

Query: 167 ETWALADYYGKLDLVRNET 185
           E    A  YG  +  + ++
Sbjct: 179 EVRDKARAYGLPNAAKADS 197


>UniRef50_Q0C5H6 Putative uncharacterized protein n=1 Tax=Hyphomonas neptunium ATCC
           15444 RepID=Q0C5H6_HYPNA
          Length = 456

 Score = 87.4 bits (215), Expect = 4e-16,   Method: Composition-based stats.
 Identities = 52/230 (22%), Positives = 71/230 (30%), Gaps = 43/230 (18%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
            V+ +S G DS       L      H +    G+    E  V+                 
Sbjct: 144 VVIPYSNGLDSFAVA--RLHAQPRQHIIRVSTGRVGDTEKKVS------------HARTA 189

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA-YQVKAEAVITG 122
           +        SL    + +P+    +          R  LF  +AA  A     AE V+  
Sbjct: 190 STRWVSMPISLPGAGVRLPEQSYRS----------RGFLFGAVAATAAGIMKGAEIVVPE 239

Query: 123 VCETDF--------SGYPDCR--DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALA 172
             +  F          +PD R    F  AL   VS      IRF  P +W  K ET A A
Sbjct: 240 SGQGTFGPALTVIGHEHPDVRMSPIFTSALGRFVSRIFESPIRFVHPRVWSTKGETLA-A 298

Query: 173 DYYGKLDLVRNETLTCYNGFK-------GDGCGHCAACNLRANGLNHYLA 215
               +L      T++C    +          CG CA C LR   L     
Sbjct: 299 LQIAQLSEGWEVTVSCSRSSRRKIQGSVAKHCGVCANCLLRRQSLQVAGL 348


>UniRef50_A8ES35 tRNA-specific 2-thiouridylase mnmA 1 n=5 Tax=Epsilonproteobacteria
           RepID=MNMA1_ARCB4
          Length = 345

 Score = 87.0 bits (214), Expect = 4e-16,   Method: Composition-based stats.
 Identities = 27/180 (15%), Positives = 48/180 (26%), Gaps = 13/180 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR----HRAEIDVARELALKLGARA 57
           K+ +V  SGG DS+        +  EV  +      R    H   +    ++A  L  + 
Sbjct: 4   KKVMVGMSGGIDSSVTAYMLQNEGYEVEGIYLKLHNRTDGYHEKNLGYIEDVAKFLNIKY 63

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
           + +      ++                       PN  V     +       +A Q  A 
Sbjct: 64  YILDLADKFSKEVYDYFVDS--------YLTGTTPNPCVKCNRQIKFGAMLDFAKQHGAS 115

Query: 118 AVITGVCE-TDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            + TG    TD   + +  D+          +          PL    K +         
Sbjct: 116 YLATGHYAKTDGKFFYEADDKTKDQSYFLSQVDKDALKYMMFPLSTYKKEDIVKFGAKLD 175


>UniRef50_Q47DY3 Putative uncharacterized protein n=5 Tax=Proteobacteria
           RepID=Q47DY3_DECAR
          Length = 457

 Score = 87.0 bits (214), Expect = 5e-16,   Method: Composition-based stats.
 Identities = 41/244 (16%), Positives = 68/244 (27%), Gaps = 42/244 (17%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           AV +FSGG DS    +  L+       V+      H  E   +             VL  
Sbjct: 150 AVSLFSGGLDSLIGAIDLLEAGRTPLLVS------HAGEGATSDAQ---------NVLFA 194

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPN-TFVPGRNILFLTLAAIYAYQVKAEAVIT- 121
            L    +  +  R  + +        G+ +     GR+ LF  L       +  + V+  
Sbjct: 195 ALKKHFSAVAFDRLRVWMLFPADLVQGVSSENSTRGRSFLFFALGVFAGTGLGTDFVLRV 254

Query: 122 ---GVCETDFSGYP---------DCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW 169
              G+   +    P              ++   N  +        R E P     K E  
Sbjct: 255 PENGLIALNVPLDPLRLGSNSTRTTHPYYIARWNDLLGHLGISG-RVENPYWAKTKGEMA 313

Query: 170 ALADYYGKLDLVRNETLTCYNGFKGDG----------CGHCAACNLRANGLNHYLAD--K 217
           +       L  +    L+C +                CG+C  C +R   L+        
Sbjct: 314 SACANPVLLKALAPYALSCAHAANARHLGIKGRGIEQCGYCLPCLIRRAALDAAWGRGND 373

Query: 218 PTVM 221
            T  
Sbjct: 374 TTPY 377


>UniRef50_A5INK8 ExsB family protein n=6 Tax=Thermotogaceae RepID=A5INK8_THEP1
          Length = 268

 Score = 87.0 bits (214), Expect = 5e-16,   Method: Composition-based stats.
 Identities = 38/177 (21%), Positives = 58/177 (32%), Gaps = 34/177 (19%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           K+ VV+FSGG DST       +   +    +T D     R EI+ A+ LA  +G R   +
Sbjct: 15  KKVVVMFSGGVDSTLLAKLTHEALGENSVALTIDSPVIPRKEIEKAKRLAGLIGIRHEFI 74

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                LNEL    L  +    PD       + ++ V             +A +   E + 
Sbjct: 75  ----ELNELKSRHLIEN---PPDRCYLCRKLRDSVVKN-----------WAKEHGFEVIA 116

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI--DKAETWALADYY 175
            G+  +D     D R          V            P +     K E    +   
Sbjct: 117 DGLNFSDLQ---DYRPG--------VKASTEDG--IWHPFIEFEVTKEEIRDFSRKL 160


>UniRef50_D1N0N9 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Victivallis vadensis ATCC BAA-548 RepID=D1N0N9_9BACT
          Length = 358

 Score = 87.0 bits (214), Expect = 5e-16,   Method: Composition-based stats.
 Identities = 39/202 (19%), Positives = 60/202 (29%), Gaps = 25/202 (12%)

Query: 1   MK-RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRA-----------EIDVARE 48
           MK + +V  SGG DS+   V A Q   EV  VT                    +      
Sbjct: 1   MKEKVIVALSGGVDSSVAAVLAQQAGCEVIGVTLRLKHPDPEFSAAQLCASKNDEAAVES 60

Query: 49  LALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
           +  KLG   H +                  +     E      PN        +      
Sbjct: 61  ICAKLGIEHHYLELFPEFECKV--------LRPAAQEYARGRTPNPCCECNYRIKFGKLV 112

Query: 109 IYAYQVKAEAVITGV---CETDFSGYPDCRDEFVKALNHAVSLGMAKDIR--FETPLMWI 163
            +A  V A  V+TG           Y   R + +K         + + I      P+  +
Sbjct: 113 EFARSVGAAKVLTGHYAKLSEHGGVYELRRGDDLKKDQSYFLYRLDQAILSMVRFPVGAM 172

Query: 164 DKAETWALADYYGKLDLVRNET 185
           DK E   +A  YG +   + ++
Sbjct: 173 DKGEVRRIAAEYGLVTSDKPDS 194


>UniRef50_Q7URE0 Putative uncharacterized protein n=1 Tax=Rhodopirellula baltica
           RepID=Q7URE0_RHOBA
          Length = 316

 Score = 87.0 bits (214), Expect = 5e-16,   Method: Composition-based stats.
 Identities = 37/213 (17%), Positives = 59/213 (27%), Gaps = 50/213 (23%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           M   VV FSGG DS+     A +   +V  VT       R ++D AR +A ++G     V
Sbjct: 28  MGGLVVAFSGGVDSSVVAAAAHRTGRDVVAVTAQSPAVARWQLDWARRVAEQVGITHQFV 87

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                         +  S    ++         T                A Q  A  ++
Sbjct: 88  STDE----------SDRSSYRRNHSDRCFYCKQT--------LYEFLTPIAEQRGA-TIV 128

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWI--DKAETWALADYYGKL 178
           +G    D     D R                   R +TPL  +   K+    LA ++   
Sbjct: 129 SGTNADDLG---DHRPGITA----------GTQARVQTPLADLGIGKSAVRQLAAHFDLE 175

Query: 179 DLVRNETLTCYNGFKGDGCGHCAACNLRANGLN 211
           +     +                 C       +
Sbjct: 176 NADLPAS----------------PCLASRIAYH 192


>UniRef50_C0E8G4 Putative uncharacterized protein n=1 Tax=Clostridium methylpentosum
           DSM 5476 RepID=C0E8G4_9CLOT
          Length = 352

 Score = 86.6 bits (213), Expect = 5e-16,   Method: Composition-based stats.
 Identities = 34/190 (17%), Positives = 59/190 (31%), Gaps = 26/190 (13%)

Query: 5   VVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR------------AEIDVARELALK 52
           ++  SGG DS+      ++Q  EV   TF   +                +I+ ARE+  +
Sbjct: 1   MIGMSGGVDSSVAAALLIEQGYEVVGATFRLWKPEDDLFDSEATCCSLDDINDAREVCHQ 60

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
           LG   + +       E  V +           E +A   PN  +     +        A 
Sbjct: 61  LGIPHYVLNLKREFKEAVVQNFVE--------EYQAGRTPNPCIVCNQKIKFDYFYQKAA 112

Query: 113 QVKAEAVITGVCE----TDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKA 166
            +  + V TG        +   +      + +        G+          PL    K 
Sbjct: 113 ALGFDYVATGHYARIEQNEQGEFQLKTAAYAQKDQSYFLYGIPYPNLGHILFPLGGYTKP 172

Query: 167 ETWALADYYG 176
           +   LA+ YG
Sbjct: 173 QVRELAEKYG 182


>UniRef50_A4RU81 Predicted protein n=3 Tax=Ostreococcus RepID=A4RU81_OSTLU
          Length = 714

 Score = 86.6 bits (213), Expect = 6e-16,   Method: Composition-based stats.
 Identities = 30/188 (15%), Positives = 55/188 (29%), Gaps = 22/188 (11%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
             V  SGG DS         Q  +V  +  DY  R  +  +   +       R     + 
Sbjct: 256 IAVSLSGGVDSMVLAYILKHQGYDVVTLHIDYKNRPESTEEA--DFVDDWSLRHGMKFER 313

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITGV 123
             ++++      R+   +                 R   +    A    +    AV+ G 
Sbjct: 314 CTVDQIRRGVTPREQYEIESRRI------------RYGFYKEAGA----KHGFPAVLLGH 357

Query: 124 CETDFSGYPDC---RDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG-KLD 179
              D          R   + ++N     G+ + +R   P++   K +    A  YG    
Sbjct: 358 HHGDVQENIITNLMRGANLLSVNGMSDEGVVEGVRIWRPMLSHVKDDVLTFAHAYGIPYF 417

Query: 180 LVRNETLT 187
           L    T +
Sbjct: 418 LDSTPTWS 425


>UniRef50_C2KV29 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=6
           Tax=Bacteria RepID=C2KV29_9FIRM
          Length = 362

 Score = 86.6 bits (213), Expect = 6e-16,   Method: Composition-based stats.
 Identities = 34/205 (16%), Positives = 66/205 (32%), Gaps = 28/205 (13%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-----YGQ----RHRA---EIDVARE 48
           M++A++  SGG DS+       ++  +   +T        GQ    R      +++ AR+
Sbjct: 1   MEKALIAMSGGVDSSVAAALMQEKGYDCIGITMKLYDSETGQACRSRTCCTLEDVEDARQ 60

Query: 49  LALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAA 108
           +A  L    + +       +  +               E  G PN  +     +      
Sbjct: 61  VASTLSMPFYVLNFKDDFQKKVILPFVET--------YENGGTPNPCIRCNRFMKHDKLY 112

Query: 109 IYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLM 161
             A ++    ++TG            R    KA +           L   +      PL 
Sbjct: 113 QRAEELSCSYIVTGHYARIEKDESSGRYLLKKAKDEKKDQSYVLYFLSQEQLAHTLFPLG 172

Query: 162 WI-DKAETWALADYYGKLDLVRNET 185
               K E   +AD YG ++  + ++
Sbjct: 173 EYSSKEEVREIADKYGFINSDKPDS 197


>UniRef50_A7I2L9 tRNA-specific 2-thiouridylase mnmA n=19 Tax=Campylobacter
           RepID=MNMA_CAMHC
          Length = 368

 Score = 86.6 bits (213), Expect = 6e-16,   Method: Composition-based stats.
 Identities = 22/190 (11%), Positives = 47/190 (24%), Gaps = 14/190 (7%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQR---HRAEIDVARELALKLGARAHK 59
           + +V  SGG DS            E+         R   H   I+  +++   L  + H 
Sbjct: 2   KILVALSGGVDSAVSAKILKDAGYEIEGCYMKLHGRDDYHAKNIEKVQDVGNFLEIKTHI 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +               +         P A             +       +A     + +
Sbjct: 62  LDLCDDFKREVFEPFLQTYKVGKTPNPCAL--------CNRTIKFGKFLDFARSKGCDKI 113

Query: 120 ITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETWALADYYGK 177
            TG                +          +   +      PL  + K +    A  + +
Sbjct: 114 ATG-HYAKIENNLIKSAVDLSKDQSYFLANIEPRVIPNIVFPLGNMLKKDVKKFAASFPE 172

Query: 178 LDLVRNETLT 187
           L+ +   + +
Sbjct: 173 LEKISKSSES 182


>UniRef50_C6J374 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Bacillales RepID=C6J374_9BACL
          Length = 410

 Score = 86.6 bits (213), Expect = 7e-16,   Method: Composition-based stats.
 Identities = 42/186 (22%), Positives = 69/186 (37%), Gaps = 32/186 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YG----QRHRAEIDVARELALKLG-AR 56
           +A+++ SGG DS      AL++  E+ CV F  Y     +  +  ID+A  LA   G  +
Sbjct: 180 KAMLLLSGGIDSPVAGWSALRRGLEIECVHFHSYPFTSKKAEQKVIDLANVLAQYSGFIK 239

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H V              T     +     +   I       R    L +A   A +  A
Sbjct: 240 LHLV------------PFTEIQTKLAQTGQDNLIITM----MRRA-MLRIATKLAERNGA 282

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A++TG      +          + L+   ++G   ++    PL+  DK +  A+A   G
Sbjct: 283 LALVTGDSLGQVAS---------QTLSSMNAIGRVTNLPILRPLVMTDKVDIIAMAQQIG 333

Query: 177 KLDLVR 182
             DL  
Sbjct: 334 TYDLSI 339


>UniRef50_Q9ZJQ0 tRNA-specific 2-thiouridylase mnmA n=22 Tax=Campylobacterales
           RepID=MNMA_HELPJ
          Length = 342

 Score = 86.3 bits (212), Expect = 7e-16,   Method: Composition-based stats.
 Identities = 31/186 (16%), Positives = 54/186 (29%), Gaps = 20/186 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG---QRHRAEIDVARELALKLGARAHK 59
           +  V+ SGG DS+       +Q  E+  +        ++H   I  A++    LG     
Sbjct: 2   KIAVLLSGGVDSSYSAYSLKEQGHELVGIYLKLHASEKKHDLYIKNAQKACEFLGIPLEV 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +                         P A   P        ++   LA  +A ++  E +
Sbjct: 62  LDFQKDFKSAVYDEFINAYEEGQTPNPCALCNP--------LMKFGLALDHALKLGCEKI 113

Query: 120 ITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKAETWALA 172
            TG          D      +AL+          +L      +   PL  + K +   LA
Sbjct: 114 ATGHYA--RVKEIDKVSYIQEALDKTKDQSYFLYALEHEVIAKLVFPLGDLLKKDIKPLA 171

Query: 173 DYYGKL 178
                 
Sbjct: 172 LNAMPF 177


>UniRef50_A6LQW7 ExsB family protein n=4 Tax=Clostridium RepID=A6LQW7_CLOB8
          Length = 274

 Score = 86.3 bits (212), Expect = 7e-16,   Method: Composition-based stats.
 Identities = 44/178 (24%), Positives = 68/178 (38%), Gaps = 36/178 (20%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           + V+ FSGG DST  L  A +   D V  VT       + EI  AREL  +LG + H+++
Sbjct: 19  KVVLAFSGGVDSTFLLRVAKEALGDNVKAVTILSPYIPKWEIAEARELVRELGVQ-HEII 77

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
           +  +++ +  +              +   +  T V        T+    A +     VI 
Sbjct: 78  EAPIIDSIKFNP------------EDRCYLCKTAV-------FTMILNVAKEQGYNCVID 118

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMW--IDKAETWALADYYGK 177
           G    D S   D R   +KAL               +PL+   + KAE  A +   G 
Sbjct: 119 GTNFDDIS---DYRPG-LKALKEL---------EVRSPLLECKLTKAEIRAFSKELGL 163


>UniRef50_B8D4I6 tRNA sulfurtransferase n=1 Tax=Desulfurococcus kamchatkensis 1221n
           RepID=THII_DESK1
          Length = 482

 Score = 86.3 bits (212), Expect = 7e-16,   Method: Composition-based stats.
 Identities = 42/188 (22%), Positives = 64/188 (34%), Gaps = 22/188 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           RA+V+FSGG DS        ++  EV  + +  G         A  +A KL         
Sbjct: 174 RALVLFSGGFDSPVAAWMIAKRGVEVDFLHYVMGS--SEVSRQAFSVARKLSEE------ 225

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
              L+      +T D  P+     E        V  R  L   +A   A ++    ++TG
Sbjct: 226 --WLSSYNPRFITVDFTPLIAEIEERIEWSYRQVVLR-ALMYMVADKIATELGYNTIVTG 282

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIR--FETPLMWIDKAETWALADYYGKLDL 180
              +  S          + L + V++  A   R     PL+  DK E    +   G  D 
Sbjct: 283 EALSQASS---------QTLANLVAVESAVSPRSIILRPLIGFDKEEIIEYSRRIGLYDY 333

Query: 181 VRNETLTC 188
                 TC
Sbjct: 334 SSRVAETC 341


>UniRef50_Q7U320 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Helicobacteraceae
           RepID=MNMA_HELHP
          Length = 345

 Score = 86.3 bits (212), Expect = 8e-16,   Method: Composition-based stats.
 Identities = 29/186 (15%), Positives = 53/186 (28%), Gaps = 20/186 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF---DYGQRHRAEIDVARELALKLGARAHK 59
           +  ++ SGG DS+ C      Q  EV  +     D  ++H   I    ++A  L      
Sbjct: 2   KVALLMSGGVDSSYCAHLLSSQGYEVIGIYLKLHDKNKKHDIYIANCEQVAAHLHIDFQV 61

Query: 60  VLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAV 119
           +       +    +            P A   P        ++   L    A ++  + +
Sbjct: 62  LDLREEFKKSVYDTFVSSYKEGKTPNPCAICNP--------LMKFGLGLQKALELGCDYI 113

Query: 120 ITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKAETWALA 172
            TG          +      KA++          +L      R   PL  + K +    A
Sbjct: 114 ATGHYA--QIKEVNGIKRIAKAVDESKDQSYFLYALPQEAIDRIIFPLGALLKEDIKKTA 171

Query: 173 DYYGKL 178
                 
Sbjct: 172 LELLPF 177


>UniRef50_B5DNY9 GA22395 n=14 Tax=Neoptera RepID=B5DNY9_DROPS
          Length = 387

 Score = 86.3 bits (212), Expect = 8e-16,   Method: Composition-based stats.
 Identities = 40/218 (18%), Positives = 62/218 (28%), Gaps = 32/218 (14%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH--------RAEIDVARELALK 52
           ++  VV  SGG DS        ++   V  V       H          ++  A     +
Sbjct: 2   IRNIVVGMSGGVDSAVSAHLLRERGHRVMGVFMRNWDEHDEAGRCSGEQDLKDAEWACQR 61

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA- 111
           LG    +V  V        S+   D         +    PN  +     +   L   +A 
Sbjct: 62  LGIELRQVNYVKEYWTAVFSAFLDD--------YQQGLTPNPDILCNRHIKFDLFHRHAL 113

Query: 112 YQVKAEAVITGV-----------CETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFET 158
            ++  +AV TG               D                     G+ +    R   
Sbjct: 114 DKLGYDAVATGHYARNSLGNFLEHRADGGEARLLIPADTFKDQTFFLAGIPRQALQRTMF 173

Query: 159 PLMWIDKAETWALADYYG--KLDLVRNETLTCYNGFKG 194
           PL  + K E   LA   G  +L   R  T  C+ G + 
Sbjct: 174 PLGDLAKNEVKLLARRIGLQRLAHKRESTGICFVGKRD 211


>UniRef50_Q8TWX3 ATP pyrophosphatase involved in thiamine biosynthesis n=1
           Tax=Methanopyrus kandleri RepID=Q8TWX3_METKA
          Length = 374

 Score = 86.3 bits (212), Expect = 8e-16,   Method: Composition-based stats.
 Identities = 35/183 (19%), Positives = 58/183 (31%), Gaps = 21/183 (11%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGAR-AHKVL 61
           R V + SGG DS      A ++  EV  +  D G ++ +E+D    LA K        V 
Sbjct: 167 RVVCLVSGGIDSPVAAWYAARRGCEVIWLHLDRG-KYGSEVDAVERLAGKFSEWLPSDVE 225

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
            +    E  + SL         Y                   +  A      V A AV+ 
Sbjct: 226 LLIEDFEDFMESLEGLEGESARYRCVLCKRE----------MIRRACDVCENVGAVAVVM 275

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLV 181
           G      +       + +  ++               PL+  DK E   L++  G  ++ 
Sbjct: 276 GDVIGQVASQI---PDNLAVIDRVAR------FPVFRPLLGFDKNEVQRLSERLGLFEVS 326

Query: 182 RNE 184
           +  
Sbjct: 327 KEH 329


>UniRef50_D0WIU4 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=1
           Tax=Slackia exigua ATCC 700122 RepID=D0WIU4_9ACTN
          Length = 362

 Score = 86.3 bits (212), Expect = 8e-16,   Method: Composition-based stats.
 Identities = 40/207 (19%), Positives = 64/207 (30%), Gaps = 33/207 (15%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG-------------QRHRA---EIDV 45
           KR +V  SGG DS+       QQ  EV   T                  R      +++ 
Sbjct: 3   KRVMVGMSGGVDSSVTAYVLKQQGYEVIGATMRLHGSDLPGRDARSCASRTCCSLDDVED 62

Query: 46  ARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLT 105
           A+ +A +LG     +       +  +    R          E    PN  +     L   
Sbjct: 63  AKSVARRLGIAHRTIDFTDRFEDAVIKPFVRA--------YETARTPNPCIDCNRRLKFG 114

Query: 106 LAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFET 158
                A  +  + V TG          D R   V+  +          SL   +      
Sbjct: 115 SMLERALDLGCDYVATG-HYAQIVRRGD-RFALVRGADASKDQSYVLYSLTQYQLAHTLL 172

Query: 159 PLMWIDKAETWALADYYGKLDLVRNET 185
           PL  + K+E  A+A   G ++  + E+
Sbjct: 173 PLGGMRKSEVRAIAAEQGFINADKPES 199


>UniRef50_Q13XN6 Putative uncharacterized protein n=1 Tax=Burkholderia xenovorans
           LB400 RepID=Q13XN6_BURXL
          Length = 458

 Score = 86.3 bits (212), Expect = 9e-16,   Method: Composition-based stats.
 Identities = 44/240 (18%), Positives = 65/240 (27%), Gaps = 52/240 (21%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVL 61
           K   V +S G DS           DE   +                           +V 
Sbjct: 146 KTFAVAYSNGLDSRAVSAL---SGDEGEALCI-------------------------RVA 177

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                 +   S  T     +  Y        +     R   F  + AI A       ++ 
Sbjct: 178 GNRQPRKDGDSYFTHIPFNISGYRGNESSFRS-----RGFQFAAVTAIAAQLSNVTRIVV 232

Query: 122 ---GVCETD------FSGYPDCR--DEFVKALNHAVSLGMAKDIRFETPLMWIDKAETW- 169
              G           +  Y D R    F + +   +       +RFE P +W  K +T  
Sbjct: 233 PESGQGALGPVLLPLYGVYADYRNYPTFFRKMERFIKALQGYQVRFEQPRLWFTKGQTLS 292

Query: 170 ALADYYGKLDLVRNETLTCYNGFK------GDGCGHCAACNLRANGLNHYLA-DKPTVMA 222
           A     GK       T +C+   +         CG CAAC LR   L+     + P    
Sbjct: 293 AFLRIPGKSGEHLKSTRSCWQTRRVVNVGSRKQCGLCAACLLRRLSLHAAGVSEAPDTYV 352


>UniRef50_C1A7G2 tRNA-specific 2-thiouridylase MnmA n=1 Tax=Gemmatimonas aurantiaca
           T-27 RepID=C1A7G2_GEMAT
          Length = 381

 Score = 86.3 bits (212), Expect = 9e-16,   Method: Composition-based stats.
 Identities = 39/190 (20%), Positives = 58/190 (30%), Gaps = 23/190 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-------DYGQRHRAEIDV---ARELALK 52
           R +V  SGG DS+      ++   +V  VT        D   R    +D    AR +  K
Sbjct: 10  RVLVAMSGGVDSSVAAAILMEHGCDVVGVTMKLHGDGADVPDRPCCSLDATSDARRVCEK 69

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
           LG   H V ++       V   + D +     E      P   V              A 
Sbjct: 70  LGI-PHYVTNL-------VDHFSHDVLDDFVQEYARGRTPIPCVRCNTFTKFRDLLHKAD 121

Query: 113 QVKAEAVITGV---CETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAE 167
            + A  + TG               R             G+ + +  R   P+    KAE
Sbjct: 122 AIDAPWLATGHYARIGHRHGQAVMVRGLDTSKDQSYFLWGIDRPVLDRLVLPIGAQTKAE 181

Query: 168 TWALADYYGK 177
           T  +A  +G 
Sbjct: 182 TRDIARRFGL 191


>UniRef50_D1BMF0 tRNA(5-methylaminomethyl-2-thiouridylate)-methyl transferase n=3
           Tax=Veillonella RepID=D1BMF0_VEIPT
          Length = 353

 Score = 85.9 bits (211), Expect = 9e-16,   Method: Composition-based stats.
 Identities = 35/211 (16%), Positives = 64/211 (30%), Gaps = 23/211 (10%)

Query: 1   MKRAV-VVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID------VARELALKL 53
           M++ + V  SGG DS+      L+Q  +V  +T           +       A+++   L
Sbjct: 1   MEKVIAVAMSGGVDSSLTAAMLLKQGYKVFGITLWLWVSGTPYDEVPLAVTDAKKMCDFL 60

Query: 54  GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           G   H +    +           + +     E      PN  V     +   L    A +
Sbjct: 61  GIEHHVIDARDVF--------YENVVDYFVKEYAYGRTPNPCVFCNKNIKFDLMLNRALE 112

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALN-------HAVSLGMAKDIRFETPLMWIDKA 166
           + A  ++TG              E  K ++          ++          PL    K 
Sbjct: 113 LGATHMVTGHYAQVNYNEETGLYELHKGVDPTKDQSYVMYNMNQRILSHLMFPLGGQCKT 172

Query: 167 ETWALADYYGKLDLVRNETLT-CYNGFKGDG 196
           ET  LA  Y      + +++  C+       
Sbjct: 173 ETRKLAAEYDLPVAKKPDSVDICFLPHGNYQ 203


>UniRef50_Q64DI2 Thiamine biosynthesis ATP pyrophosphatase n=3 Tax=environmental
           samples RepID=Q64DI2_9ARCH
          Length = 381

 Score = 85.9 bits (211), Expect = 9e-16,   Method: Composition-based stats.
 Identities = 30/183 (16%), Positives = 61/183 (33%), Gaps = 27/183 (14%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + + + SGG DS       +++  EV  + FD           +RELA +          
Sbjct: 173 KMIALVSGGIDSPVAAWMLMRRGAEVIPLYFDL---EEFTDPKSRELAEQ---------S 220

Query: 63  VTLLNELAVSSLTRDSIP----VPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEA 118
           +  L E + S +T   +P    + +     +      +  R  +   +  + A +  A  
Sbjct: 221 INSLFEWSYSDMTAYRVPYGAVLREIASRCEPRITCVICKR--MMYRIGTLIAEKEYACG 278

Query: 119 VITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKL 178
           ++TG      +          +   + ++      +    PL+ +DK E    A   G  
Sbjct: 279 IVTGSSLGQVAS---------QTSQNMLAEMHGLGMPIYHPLIGMDKREIIDRATRIGTF 329

Query: 179 DLV 181
              
Sbjct: 330 RTD 332


>UniRef50_Q0A9U9 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Alkalilimnicola ehrlichii MLHE-1 RepID=Q0A9U9_ALHEH
          Length = 422

 Score = 85.9 bits (211), Expect = 9e-16,   Method: Composition-based stats.
 Identities = 36/188 (19%), Positives = 65/188 (34%), Gaps = 32/188 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---YGQRHRAE---IDVARELALKLGAR 56
           RA+ + SGG DS        ++   V  + F      QR  A+   I +AR+L+   G  
Sbjct: 205 RALTLLSGGIDSPVAAFLMAKRGCRVDFLHFTASPSQQRADADHTVIRLARQLSRYTGHS 264

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
              ++     +                            V  R  +  T     A +++A
Sbjct: 265 RLYMVPYDHFDMALHGDQRGHE----------------VVLFRRFVART-GEALAKRLRA 307

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A+I+G      +          + + + VS  +A  +    PL+ +DKA+  A+    G
Sbjct: 308 LALISGDSLGQVAS---------QTMENMVSTSLAVRMPIMRPLVGLDKADIVAIGRRIG 358

Query: 177 KLDLVRNE 184
             D+    
Sbjct: 359 TYDISIQP 366


>UniRef50_A2PQ54 Putative uncharacterized protein n=1 Tax=Vibrio cholerae MZO-3
           RepID=A2PQ54_VIBCH
          Length = 444

 Score = 85.9 bits (211), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 34/236 (14%), Positives = 62/236 (26%), Gaps = 29/236 (12%)

Query: 5   VVVFSGGQDSTTCLVQALQQY--DEVHCVTFDYGQRHRAEIDVARELALKLG-----ARA 57
           V +FSGG DS    +  +     D+   ++  Y    + + D+   L  K          
Sbjct: 152 VSLFSGGLDSAVGAIDMINGSNVDKPLLISHSYKGDGKKQKDIKSLLRGKFSEFSFDISP 211

Query: 58  HKVLDVT-------LLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIY 110
             +                   +L    +         D I    +P    + L      
Sbjct: 212 KIIKSRRGKTDVSMRGRSFNFIALAVLGVNALRSANNDDSISRIVIPENGYISLNPPLTR 271

Query: 111 AYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA 170
                      G   T           ++  L   ++  +  +I  E P     K E  +
Sbjct: 272 RR--------IGSHST-----RTTHPFYISRLELLLNK-VGFNISLENPYQMKTKGEMVS 317

Query: 171 LADYYGKLDLVRNETLTCYNGFKGD-GCGHCAACNLRANGLNHYLADKPTVMAAMK 225
                  +      +++C N  +    CG C  C +R   +      K     A  
Sbjct: 318 ECVDKVAIKKAVVLSVSCSNWHRKHIQCGRCVPCLIRRAAVFKAGFTKDAPYDAEN 373


>UniRef50_B1YTE9 tRNA-specific 2-thiouridylase mnmA n=310 Tax=cellular organisms
           RepID=MNMA_BURA4
          Length = 389

 Score = 85.9 bits (211), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 41/208 (19%), Positives = 68/208 (32%), Gaps = 26/208 (12%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH--------RAEIDVARELALKL 53
           +R VV  SGG DS+       +Q  +V  +     +          R +      +A  +
Sbjct: 4   RRVVVGMSGGVDSSVTAWLLKEQGYDVVGLFMKNWEDDDDGEYCSTRQDWIDVVSVADLI 63

Query: 54  GARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQ 113
           G     V       +   +   R        E  A   PN  V     +       +A  
Sbjct: 64  GIDVEAVNFAAEYKDRVFAEFLR--------EYSAGRTPNPDVLCNAEIKFKAFLDHAMS 115

Query: 114 VKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKA 166
           + AE + TG          D R E +KA +H          L  A+  +   PL  I K 
Sbjct: 116 LDAEMIATGHYA--RVRERDGRFELLKAFDHTKDQSYFLHRLNQAQLSKTMFPLGEIPKT 173

Query: 167 ETWALADYYGKLDLVRNETLT-CYNGFK 193
           +   +A   G  +  + ++   C+ G +
Sbjct: 174 KVREIAAQIGLPNAKKKDSTGICFIGER 201


>UniRef50_Q9X220 Probable tRNA sulfurtransferase n=6 Tax=Thermotogaceae
           RepID=THII_THEMA
          Length = 388

 Score = 85.9 bits (211), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 38/194 (19%), Positives = 66/194 (34%), Gaps = 33/194 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTF-DYGQRHRAEIDVARELALKL----GARA 57
           +AV++ SGG DS      AL++   +  VTF          ++  R++   L    G   
Sbjct: 176 KAVLLLSGGIDSPVAGWYALKRGVLIESVTFVSPPFTSEGAVEKVRDILRVLREFSGGHP 235

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
            ++  V          LT+  + V    P+   +    +  R  +F  +A   A +  A 
Sbjct: 236 LRLHIVN---------LTKLQLEVKKRVPDKYSL----IMYRRSMF-RIAEKIAEETGAV 281

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A  TG      +          + L +  S+          PL   DK E    A   G 
Sbjct: 282 AFYTGENIGQVAS---------QTLENLWSIESVTTRPVIRPLSGFDKTEIVEKAKEIGT 332

Query: 178 LDL---VRNETLTC 188
            ++      +  +C
Sbjct: 333 YEISIKPYQD--SC 344


>UniRef50_B1ZZ32 tRNA-specific 2-thiouridylase mnmA n=3 Tax=Verrucomicrobia
           RepID=MNMA_OPITP
          Length = 378

 Score = 85.5 bits (210), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 34/217 (15%), Positives = 61/217 (28%), Gaps = 41/217 (18%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYG---------QRHRAEIDVARELALK 52
           ++ +V  SGG DS+   +   QQ  +V                    + +I+ AR +A +
Sbjct: 5   EKILVAMSGGVDSSVAALLLKQQGYDVVGAYMKNWINEDQVIGHCPWQQDIEDARRVATQ 64

Query: 53  LGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAY 112
           +G     V  +    E  V+ L                 PN  +     +   +   +A 
Sbjct: 65  IGIEFRVVNLMREYRERVVAYLLDG--------YARGLTPNPDIMCNREVKFGVFRSWAK 116

Query: 113 QVKAEAVITGVCETDFSGYPD-CRDEFVKALNH-----------------------AVSL 148
                A+ TG         PD      +  LN                           L
Sbjct: 117 DHGFAAIATGHYAQRVEVKPDLVTPAVLGGLNADGTPASTFALLEGADKNKDQSYFLALL 176

Query: 149 GMAKDIRFETPLMWIDKAETWALADYYGKLDLVRNET 185
              +      P+  + K E   +A   G     + ++
Sbjct: 177 SQEQLRDARFPIGHLPKPELRRIAREAGLATADKKDS 213


>UniRef50_B3DUT9 tRNA(5-methylaminomethyl-2-thiouridine)-methyltransferase n=1
           Tax=Methylacidiphilum infernorum V4 RepID=B3DUT9_METI4
          Length = 367

 Score = 85.5 bits (210), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 42/212 (19%), Positives = 65/212 (30%), Gaps = 29/212 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-----------IDVARELAL 51
           R VV  SGG DST       ++  +V  +T                     I+ AR +A 
Sbjct: 13  RIVVGMSGGVDSTVAAYLLKREGFDVVGITLKIWTDRCMHISSEKCCGPDAINDARNMAA 72

Query: 52  KLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYA 111
           ++G   + + +     +  +S   R        E      PN  V     +         
Sbjct: 73  QIGIPHYVLDEADCFEKEVISYFLR--------EYSLGRTPNPCVKCNEKIKFGRLWTRV 124

Query: 112 YQVKAEAVITGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWID 164
                E + TG          D      KA +          SL   +  R  TPL  ++
Sbjct: 125 QSWGVEYLATGHYA--LVENQDHIPRLKKAKDKRRDQSYFLFSLSAGQLSRMITPLGNLE 182

Query: 165 KAETWALADYYGKLDLVRNETLT-CYNGFKGD 195
           K E  A+A   G     + E+   C+   K  
Sbjct: 183 KKEVRAIAKEAGLNVWNKEESRDICFVPGKNY 214


>UniRef50_A1U6U9 tRNA sulfurtransferase n=11 Tax=Gammaproteobacteria
           RepID=THII_MARAV
          Length = 484

 Score = 85.1 bits (209), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 38/183 (20%), Positives = 63/183 (34%), Gaps = 32/183 (17%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKV 60
           ++  + + SGG DS+      +++    H + F+ G    A     R++A  L       
Sbjct: 178 VETVMTLISGGYDSSVAAYLMMRRGIRSHYLFFNLG--GAAHEVGVRQVAHYL------- 228

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNI------LFLTLAAIYAYQV 114
                  E   SS     I VP     A+ + +  V  R+       + L  AA  A   
Sbjct: 229 ------WERYGSSHNVKFISVPFEGVVAEIMRS--VNHRHWGVVLKRMMLKAAAEIARDY 280

Query: 115 KAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADY 174
            A  ++ G      S          + L +   +  A D     PL+ +DK E   +A  
Sbjct: 281 NASGLVMGDAVAQVSS---------QTLTNLNVVDRASDEVVLRPLIAMDKQEIIRIAKD 331

Query: 175 YGK 177
            G 
Sbjct: 332 IGT 334


>UniRef50_Q9Y933 GMP synthase [glutamine-hydrolyzing] n=5 Tax=Archaea
           RepID=GUAA_AERPE
          Length = 512

 Score = 85.1 bits (209), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 39/186 (20%), Positives = 66/186 (35%), Gaps = 23/186 (12%)

Query: 4   AVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           AV   SGG DST   + A +     ++ V  D+G     E++   +L  +LG     V  
Sbjct: 221 AVAAVSGGVDSTVAALIAKKAIGSRLYPVFIDHGLHPEGEVERVVKLLSRLGLEPVMVD- 279

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
                E  +++L     P               V GR  ++  +    A  + AE ++ G
Sbjct: 280 ---AGEEFLAALEGVGDPEEKRR----------VVGR--VYAEVLERAARDIGAEYLVQG 324

Query: 123 VCETDFSGYPDCRD--EFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
               D       R   + +K  ++   L     ++   PL +  K E   LA+    L +
Sbjct: 325 TIYPDV-IESGARPGADTIKTHHNVGGLPKDMRLKLVEPLRYFYKDEVRLLAEK---LGV 380

Query: 181 VRNETL 186
            R    
Sbjct: 381 PRELIW 386


>UniRef50_D0LKW7 Queuosine synthesis-like protein n=1 Tax=Haliangium ochraceum DSM
           14365 RepID=D0LKW7_HALO1
          Length = 296

 Score = 85.1 bits (209), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 40/177 (22%), Positives = 60/177 (33%), Gaps = 30/177 (16%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQ-YDEVHCVTFDYGQRHRAEIDVARELALKLG-ARAHKV 60
           R +V FSGG DS   L  A  +   + H +T       R+E++ AR L  +LG A  H V
Sbjct: 32  RVLVAFSGGVDSAFLLKIASDELGADCHALTCVSVTMARSEVEDARALGDELGLAERHHV 91

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           ++   L +   +        +   E      P                  A Q+  E + 
Sbjct: 92  VESNELEQPGFADNPTHRCALCKTELMDVARP-----------------LAEQLGVEVIA 134

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
            G    D     D R     A   A  + M +          + KA+  AL+   G 
Sbjct: 135 IGTNIDDLG---DFRPGIAAAKERAARMPMVE--------AGLSKADIRALSRQLGL 180


>UniRef50_Q5NVV1 Thiamine biosynthesis protein (ATP pyrophosphatase) n=3 Tax=Archaea
           RepID=Q5NVV1_9ARCH
          Length = 391

 Score = 85.1 bits (209), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 31/186 (16%), Positives = 64/186 (34%), Gaps = 18/186 (9%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLD 62
           + V + SGG DS       +++  E+  +  D    +  E   A+ +   +  + H+   
Sbjct: 182 KMVALISGGIDSPVAAWLMMKRGCEIIPLYMD-NTPYLGEDAKAKTI--DIVRKLHEWAP 238

Query: 63  VTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVITG 122
              +       +     P+ +   +      T V  ++ L   +A   A +  A  +ITG
Sbjct: 239 GREMKMY----IAPHGQPLRE-FLQKGNKKYTCVFCKH-LMYKVARAIAEKEGAHGIITG 292

Query: 123 VCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDLVR 182
                 +          +   + ++     D     PL+ +DK E   +A   G  D+  
Sbjct: 293 SSLGQVAS---------QTSENMMAEAYDVDFPIYHPLIGLDKEEIMTIAKKIGTYDISI 343

Query: 183 NETLTC 188
            +   C
Sbjct: 344 RKAGGC 349


>UniRef50_A6KZ28 tRNA-specific 2-thiouridylase mnmA 2 n=10 Tax=Bacteroides
           RepID=MNMA2_BACV8
          Length = 351

 Score = 85.1 bits (209), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 32/186 (17%), Positives = 54/186 (29%), Gaps = 17/186 (9%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR--AEIDVARELALKLGARAHKV 60
           + ++  SGG DS+   +       EV  +TF + ++      ++ AR L  +L       
Sbjct: 9   KVLLGMSGGTDSSVAALLLQDAGYEVTGITFRFYEKENDTEYLEDARALCERLNIPHLTY 68

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                          +  I     E  A   P       N L   L    + ++      
Sbjct: 69  DVRDTFR--------KTIIDYFINEYMAGHTPVPCTLCNNYLKWPLLKKISDEMGIYHFA 120

Query: 121 TGVCETDFSGYPDCRDEFV----KALNHAVSLGMAKDI--RFETPLMWIDKAETWALADY 174
           TG           C                  G+ ++I  R   P+  + KA    +A  
Sbjct: 121 TG-HYVRRRFINGCYHITTGVDPDKDQSFFLWGLPQEILQRMLLPMGNLTKARVREIAAE 179

Query: 175 YGKLDL 180
            G L  
Sbjct: 180 RGFLKA 185


>UniRef50_D1N6V4 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Victivallis vadensis ATCC BAA-548 RepID=D1N6V4_9BACT
          Length = 400

 Score = 85.1 bits (209), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 38/180 (21%), Positives = 62/180 (34%), Gaps = 24/180 (13%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAE-IDVARELALKLG-ARAHKV 60
           + + + SGG DS     Q +++      +TF        E +D  R +A  L   + H V
Sbjct: 192 KVLTLLSGGIDSPVAAWQIMKRGCPTDFITFHSAPYTPPETVDKVRGIAEHLNTFQQHGV 251

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
           L +  L             P+     +        V  R    L +A   A      A++
Sbjct: 252 LHLCNLA------------PMQKLIRDNCNERFRTVLYRRA-MLRIAEQVAKNTGCRALV 298

Query: 121 TGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLDL 180
           TG      +         +  +N AV +          PL+  DK E+  +A   G L+L
Sbjct: 299 TGEAVGQVA---SQTIVNMNTINRAVDML------VLRPLVGADKLESIRIAGEIGTLEL 349


>UniRef50_Q17440 Probable mitochondrial tRNA-specific 2-thiouridylase 1 n=2
           Tax=Chromadorea RepID=MTU1_CAEEL
          Length = 375

 Score = 85.1 bits (209), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 35/206 (16%), Positives = 57/206 (27%), Gaps = 37/206 (17%)

Query: 1   MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTF---------DYGQRHRAEIDVARELAL 51
           M R V+  SGG DS        ++  +V  +                   +   AR +  
Sbjct: 1   MPRVVIGMSGGVDSAVSAFLLKKRGFDVIGLHMINWDVQEEGTSHCPRSKDESDARNVCD 60

Query: 52  KLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVP---GRNILFLTLAA 108
           +L    H V  V                 V     E      T VP       +   +  
Sbjct: 61  RLNIPFHTVNFVKEYWN-----------DVFLKFLENYKNGRTTVPDIDCNQSIKFDVFH 109

Query: 109 IYA-YQVKAEAVITG----VCETDF---SGYPDCRDEFVKA---LNHAVSLGMAKDIRF- 156
             A  +  A+ + TG        DF   +   D    F       +    L      +  
Sbjct: 110 KIAREKFNADFIATGHYATTNFGDFQQNAKDSDEIRLFSGKDPLKDQTFFLCTVNQEQLK 169

Query: 157 --ETPLMWIDKAETWALADYYGKLDL 180
               PL  + K+E   +A+  G  ++
Sbjct: 170 RAMFPLGSLQKSEVKRIAEEQGFQEV 195


>UniRef50_Q09580 Probable GMP synthase [glutamine-hydrolyzing] n=4 Tax=Chromadorea
           RepID=GUAA_CAEEL
          Length = 792

 Score = 84.7 bits (208), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 34/190 (17%), Positives = 57/190 (30%), Gaps = 14/190 (7%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHK 59
           K+ +V+ SGG DS  C     +      V  +  D G     E D   +    L    H+
Sbjct: 320 KKVLVMVSGGVDSAVCAALLRRALGPNRVTAIHIDNGFMRHEESDAVEKSLAALDLPIHR 379

Query: 60  VLDVTLLNELAVSSLTRD--SIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
               T            +       D E +   I NTF+  ++++   L           
Sbjct: 380 YNFGTTFRSSTEHCKDGEVALDECDDPEMKRKIIGNTFIRVKDVIMKDLN----INHDEY 435

Query: 118 AVITGVCETD----FSGYPDCRDEFVKAL--NHAVSLGMAKDIRFETPLMWIDKAETWAL 171
            +  G    D     S       + +K    +  +   + K  +   PL    K E   L
Sbjct: 436 FLAQGTLRPDLIESASALASGHADTIKTHHNDTFLVRELRKLGKVVEPLKDFHKDEVREL 495

Query: 172 ADYYGKLDLV 181
               G  + +
Sbjct: 496 GKDLGLPESI 505


>UniRef50_C6D5K7 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Paenibacillus RepID=C6D5K7_PAESJ
          Length = 406

 Score = 84.7 bits (208), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 32/187 (17%), Positives = 60/187 (32%), Gaps = 24/187 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS      A+++  E+  V F  Y        +   +LA +L        
Sbjct: 179 KAMLLLSGGIDSPVAGWMAMRKGLELEAVHFHSYPFTSDKAKEKVIDLARRL-------- 230

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
             +      +         +     +A          R    L +    A +  A  ++T
Sbjct: 231 --SYYAGAPLKLHLVPFTDIQTRLVQAKQDSMMITLMRRA-MLRITEQLADRNGAGGIVT 287

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD-- 179
           G      +          + L+    +G A  +    PL+ +DK E    A   G  +  
Sbjct: 288 GESLGQVAS---------QTLSSMNVIGRATVLPLLKPLIMMDKQEIINEAVRIGTFETS 338

Query: 180 -LVRNET 185
            L   + 
Sbjct: 339 ILPYEDC 345


>UniRef50_B3JKC8 Putative uncharacterized protein n=2 Tax=Bacteroides
           RepID=B3JKC8_9BACE
          Length = 353

 Score = 84.7 bits (208), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 38/187 (20%), Positives = 58/187 (31%), Gaps = 19/187 (10%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHR--AEIDVARELALKLGARAHKV 60
           + ++  SGG DS+   +       EV  VTF + ++      +D AREL  +L    H  
Sbjct: 6   KVLLGMSGGTDSSVAALLLQDAGYEVTGVTFRFYEKDNNTEYLDDARELCERLHIS-HIT 64

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
            D        + S   +       E      P       N L   L    A +       
Sbjct: 65  YDARNEFRERIISYFIN-------EYMTGRTPVPCTLCNNYLKWPLLKKLADEKGIYHFA 117

Query: 121 TGV-----CETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETWALAD 173
           TG          +       D  V         G+ +DI  R   P+  + K +   +A 
Sbjct: 118 TGHYVQKRNIDGYWHITTGTD--VDKDQSFFLWGLPQDILQRMLLPMGKLTKNKVREIAA 175

Query: 174 YYGKLDL 180
             G L  
Sbjct: 176 ERGFLKA 182


>UniRef50_A6TQ96 Probable tRNA sulfurtransferase n=14 Tax=Clostridia
           RepID=THII_ALKMQ
          Length = 392

 Score = 84.7 bits (208), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 31/178 (17%), Positives = 63/178 (35%), Gaps = 22/178 (12%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD-YGQRHRAEIDVARELALKLGARAHKVL 61
           +A+++ SGG DS        ++  E+H + F  Y        +   +LA  L        
Sbjct: 179 KALLLLSGGIDSPVAGWLVAKRGVEIHGMHFHSYPFTSERAKEKVVDLAKIL-------T 231

Query: 62  DVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT 121
                 +L   +L      + +  PE +    T +  R    + +A   A ++  +A++T
Sbjct: 232 TYCGRIKLYSVNLLAIQKEINEKCPEEE---MTILSRR--FMMKIAERVANKIGCDALVT 286

Query: 122 GVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGKLD 179
           G      +          + +        A ++    PL+ +DK +   LA      +
Sbjct: 287 GESIGQVAS---------QTVKGLHVTNAAVELPVFRPLIAMDKVDIMDLARKIDTYE 335


>UniRef50_Q65G52 Probable tRNA sulfurtransferase n=20 Tax=Bacillales
           RepID=THII_BACLD
          Length = 401

 Score = 84.7 bits (208), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 36/186 (19%), Positives = 61/186 (32%), Gaps = 32/186 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---YGQRHRAE--IDVARELALKLG-AR 56
           +A+++ SGG DS      A+++  E+  V F    Y      +  ID+ + L    G  +
Sbjct: 179 KAMLMISGGIDSPVAGFYAMKRGLEIEAVHFFSPPYTSERAKQKVIDLTKRLTAFGGDIK 238

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H V              T+    +    PE   +  T       L L +A     +  A
Sbjct: 239 LHIV------------PFTKTQELIQKQIPENYSMTAT-----RRLMLQIADKLRERHNA 281

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A+ TG      +          + L    ++          PL+ +DK E    A    
Sbjct: 282 LAIFTGESLGQVAS---------QTLESMYAINAVTSTPVLRPLIGMDKTEIIEKAKEID 332

Query: 177 KLDLVR 182
             D+  
Sbjct: 333 TYDISI 338


>UniRef50_D2MP56 Thiamine biosynthesis/tRNA modification protein ThiI n=1
           Tax=Bulleidia extructa W1219 RepID=D2MP56_9FIRM
          Length = 472

 Score = 84.3 bits (207), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 35/191 (18%), Positives = 62/191 (32%), Gaps = 33/191 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD---YGQRHRAE--IDVARELALKLGARA 57
           + +++ SGG DS     Q +++  +V  V F    Y         + +A  ++   G   
Sbjct: 177 KVMMLLSGGIDSPVATYQLIKRGLKVEAVHFAAPPYTSEAAKNKVLKLASMISDYQGGMK 236

Query: 58  HKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAE 117
             V+  T L              +  Y    D    T +     +   +A   A   +  
Sbjct: 237 VHVVPFTELQ-------------LDIYRVAKDPYAITLMR---RMMFRIAEKLAINNRCL 280

Query: 118 AVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYGK 177
           A+ TG      +          + L   V++     I    PL+ +DK E   LA   G 
Sbjct: 281 AIGTGESLGQVAS---------QTLESMVTINDVVHIPVLRPLVSMDKVEIIQLAREIGT 331

Query: 178 LD---LVRNET 185
            +   L   + 
Sbjct: 332 YETSILPYEDC 342


>UniRef50_Q1NYA2 TRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase n=3
           Tax=Deltaproteobacteria RepID=Q1NYA2_9DELT
          Length = 443

 Score = 84.3 bits (207), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 36/192 (18%), Positives = 53/192 (27%), Gaps = 17/192 (8%)

Query: 2   KRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRH-RAEIDVARELALKLGARAHKV 60
           ++  V  SGG DS+       Q   EV  V     Q    A    A  +A  LG     +
Sbjct: 76  RQVAVAMSGGVDSSVTAALLQQAGAEVRGVFMRLAQPEVEAHRQRAAAVADFLGIELTVL 135

Query: 61  LDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVI 120
                  +  ++               A   PN  V    ++   L          E + 
Sbjct: 136 DLAQPFRQQVLNPFVEA--------YLAGLTPNPCVFCNRLIKFGLLLEILLAQGVELLA 187

Query: 121 TGVCETDFSGYPDCRDEFVKALNH-------AVSLGMAKDIRFETPLMWIDKAETWALAD 173
           TG                 + L+           L   K  R   PL    K+E + LA 
Sbjct: 188 TG-HYVRLLPAATGGRRLCRGLDPGKDQSYFLCRLAAEKLPRLLFPLGDKHKSEVYQLAA 246

Query: 174 YYGKLDLVRNET 185
             G      +E+
Sbjct: 247 RLGLSGRHGSES 258


>UniRef50_C8WW19 Thiamine biosynthesis/tRNA modification protein ThiI n=2
           Tax=Alicyclobacillus acidocaldarius RepID=C8WW19_ALIAD
          Length = 400

 Score = 84.3 bits (207), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 37/186 (19%), Positives = 69/186 (37%), Gaps = 32/186 (17%)

Query: 3   RAVVVFSGGQDSTTCLVQALQQYDEVHCVTFD----YGQRHRAEIDVARELALKLGA--R 56
           + +V+ SGG DS     +A+++   +  V F       +R + +++    +  K G   +
Sbjct: 179 KGLVLLSGGIDSPVAAWKAMKRGLALELVHFHSFPFTSERAQQKVEDLAAILTKWGGPMK 238

Query: 57  AHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKA 116
            H V    +  E+  S+                    T +  R  + +  A+  A +V A
Sbjct: 239 LHLVSVTAIQAEIQRSA---------------PEGLRTILLRR--MMVRAASRIADEVGA 281

Query: 117 EAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWALADYYG 176
            A++TG      +          + L+   ++  A D     PL+  DK E   LA   G
Sbjct: 282 GALVTGDSLGQVAS---------QTLSALNAVDRATDHTILRPLIAEDKLEIIELAKRMG 332

Query: 177 KLDLVR 182
             D+  
Sbjct: 333 TYDISI 338


>UniRef50_Q1AWB1 tRNA-specific 2-thiouridylase mnmA n=1 Tax=Rubrobacter xylanophilus
           DSM 9941 RepID=MNMA_RUBXD
          Length = 488

 Score = 84.3 bits (207), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 42/184 (22%), Positives = 57/184 (30%), Gaps = 18/184 (9%)

Query: 4   AVVVFSGGQDSTTCLVQALQQYDEVHCVTFDY-----GQRHRAEID---VARELALKLGA 55
            V   SGG DS    +   ++  EV  VTF       G R     D    ARE A  LG 
Sbjct: 146 VVAAMSGGVDSAVTALLLKERGYEVVAVTFRLHDGEPGSRSCCSPDTVLFARETAHGLGI 205

Query: 56  RAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVK 115
               +    L +   +                A   PN  V     +    AA  A ++ 
Sbjct: 206 PHFTLNLRELFDRRVMRDFV--------GSYAAGRTPNPCVACNAHVKFHAAAFLADRLG 257

Query: 116 AEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDI--RFETPLMWIDKAETWALAD 173
              V TG       G    R E  +     V   +   +  R   PL    K+E   +A+
Sbjct: 258 LRHVATGHYARVGEGPCLERPEDGRKDQTYVLWPVPPRLLGRTIFPLGDYRKSEVRRIAE 317

Query: 174 YYGK 177
             G 
Sbjct: 318 ERGL 321


>UniRef50_B9B2T1 Putative uncharacterized protein n=2 Tax=Burkholderia
           RepID=B9B2T1_9BURK
          Length = 468

 Score = 83.9 bits (206), Expect = 3e-15,   Method: Composition-based stats.
 Identities = 46/237 (19%), Positives = 68/237 (28%), Gaps = 44/237 (18%)

Query: 6   VVFSGGQDSTTCLVQALQQYD--EVHCVTFDYGQRHRAEIDVARELALKLGARAHKVLDV 63
           V FS G DS   +    +++   EV CV  D               A   G      L  
Sbjct: 151 VPFSHGLDSYGQVRLWQREHPDTEVVCVFAD---------------ARTSGVDHGNGLKR 195

Query: 64  TLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNILFLTLAAIYAYQVKAEAVIT-- 121
                +    +     P+   EP     P         +F  LA + A +  A  V+   
Sbjct: 196 RTSEHIQYVRVPVSVKPMRRAEPSFRSRP--------FMFYLLAGLGAIESGANKVMIPE 247

Query: 122 ---GVCETDF------SGYPDCRDEFVKALNHAVSLGMAKDIRFETPLMWIDKAETWA-L 171
              G            + +  C   F   L+  V     K ++F  P ++  K      L
Sbjct: 248 NGQGSIGGSLIVTGHEAKHRSCYPGFTTKLSRFVEALTGKRVKFYHPALFETKGAVLRAL 307

Query: 172 ADYYGKLDLVRNETLTCY-------NGFKGDGCGHCAACNLRANGLNHYLADKPTVM 221
           AD    +  V     +C           +   CG C  C LR    +    D  T  
Sbjct: 308 ADAGENVRAVLGRHRSCSCDARLTSRDEQMFHCGVCGNCLLRRTAEHSANLDGATEY 364


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.312    0.135    0.411 

Lambda     K      H
   0.267   0.0411    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,419,945,888
Number of Sequences: 3077464
Number of extensions: 55546690
Number of successful extensions: 182005
Number of sequences better than 1.0e-01: 250
Number of HSP's better than  0.1 without gapping: 1001
Number of HSP's successfully gapped in prelim test: 1770
Number of HSP's that attempted gapping in prelim test: 178279
Number of HSP's gapped (non-prelim): 2963
length of query: 231
length of database: 1,040,396,356
effective HSP length: 124
effective length of query: 107
effective length of database: 658,790,820
effective search space: 70490617740
effective search space used: 70490617740
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 91 (39.7 bits)
