Processing 1 helix: Transmembrane helix 1 from 77 (in) to 92 (out) : -77.963 Score = -0.077963 Processing 1 helix: Transmembrane helix 1 from 77 (out) to 92 (in) : -75.437 Score = -0.075437 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 23 (out) to 38 (in) : -528.737 Transmembrane helix 3 from 42 (in) to 57 (out) : -224.144 Transmembrane helix 4 from 61 (out) to 76 (in) : -223.015 Transmembrane helix 5 from 80 (in) to 95 (out) : -96.74 Transmembrane helix 6 from 99 (out) to 114 (in) : -363.784 Transmembrane helix 7 from 125 (in) to 140 (out) : -1000.43 Transmembrane helix 8 from 144 (out) to 159 (in) : -276.419 Transmembrane helix 9 from 163 (in) to 178 (out) : -402.924 Transmembrane helix 10 from 182 (out) to 197 (in) : -154.469 Transmembrane helix 11 from 201 (in) to 216 (out) : -340.583 Transmembrane helix 12 from 220 (out) to 235 (in) : -460.472 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -294.947 Transmembrane helix 2 from 23 (in) to 38 (out) : -515.163 Transmembrane helix 3 from 42 (out) to 57 (in) : -209.756 Transmembrane helix 4 from 61 (in) to 76 (out) : -216.185 Transmembrane helix 5 from 80 (out) to 95 (in) : -85.16 Transmembrane helix 6 from 99 (in) to 114 (out) : -375.616 Transmembrane helix 7 from 144 (out) to 159 (in) : -276.419 Transmembrane helix 8 from 163 (in) to 178 (out) : -402.924 Transmembrane helix 9 from 182 (out) to 197 (in) : -154.469 Transmembrane helix 10 from 201 (in) to 216 (out) : -340.583 Transmembrane helix 11 from 220 (out) to 235 (in) : -460.472 Re-entrant helix 1 from 118 (out) to 140 (out) : 1610 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 39 (out) to 54 (in) : -263.906 Transmembrane helix 3 from 58 (in) to 73 (out) : -216.968 Transmembrane helix 4 from 77 (out) to 92 (in) : -75.437 Transmembrane helix 5 from 96 (in) to 111 (out) : -282.633 Transmembrane helix 6 from 144 (out) to 159 (in) : -276.419 Transmembrane helix 7 from 163 (in) to 178 (out) : -402.924 Transmembrane helix 8 from 182 (out) to 197 (in) : -154.469 Transmembrane helix 9 from 201 (in) to 216 (out) : -340.583 Transmembrane helix 10 from 220 (out) to 235 (in) : -460.472 Re-entrant helix 1 from 23 (out) to 35 (out) : 490 Re-entrant helix 2 from 115 (out) to 140 (out) : 1820 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -294.947 Transmembrane helix 2 from 30 (in) to 45 (out) : -448.669 Transmembrane helix 3 from 49 (out) to 64 (in) : -131.549 Transmembrane helix 4 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 5 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 6 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 7 from 176 (out) to 191 (in) : -230.453 Transmembrane helix 8 from 195 (in) to 210 (out) : -236.568 Transmembrane helix 9 from 220 (out) to 235 (in) : -460.472 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 3 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 4 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 5 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 6 from 176 (out) to 191 (in) : -230.453 Transmembrane helix 7 from 195 (in) to 210 (out) : -236.568 Transmembrane helix 8 from 220 (out) to 235 (in) : -460.472 Re-entrant helix 1 from 23 (out) to 44 (out) : 1120 Re-entrant helix 2 from 106 (in) to 140 (in) : 2450 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -294.947 Transmembrane helix 2 from 30 (in) to 45 (out) : -448.669 Transmembrane helix 3 from 49 (out) to 64 (in) : -131.549 Transmembrane helix 4 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 5 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 6 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 7 from 186 (out) to 201 (in) : -127.982 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Re-entrant helix 2 from 169 (out) to 182 (out) : 980 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 3 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 4 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 5 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 6 from 186 (out) to 201 (in) : -127.982 Re-entrant helix 1 from 23 (out) to 44 (out) : 1120 Re-entrant helix 2 from 106 (in) to 140 (in) : 2450 Re-entrant helix 3 from 169 (out) to 182 (out) : 980 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 2 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 3 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 4 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 5 from 186 (out) to 201 (in) : -127.982 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Re-entrant helix 2 from 169 (out) to 182 (out) : 980 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -136.951 Transmembrane helix 2 from 77 (out) to 92 (in) : -75.437 Transmembrane helix 3 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 4 from 186 (out) to 201 (in) : -127.982 Re-entrant helix 1 from 96 (in) to 130 (in) : 2450 Re-entrant helix 2 from 131 (in) to 146 (in) : 1120 Re-entrant helix 3 from 169 (out) to 182 (out) : 980 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 2 from 77 (in) to 92 (out) : -77.963 Transmembrane helix 3 from 186 (out) to 201 (in) : -127.982 Re-entrant helix 1 from 96 (out) to 130 (out) : 2450 Re-entrant helix 2 from 131 (out) to 165 (out) : 2450 Re-entrant helix 3 from 166 (out) to 182 (out) : 1190 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -136.951 Transmembrane helix 2 from 77 (out) to 92 (in) : -75.437 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -294.947 Transmembrane helix 2 from 23 (in) to 38 (out) : -515.163 Transmembrane helix 3 from 42 (out) to 57 (in) : -209.756 Transmembrane helix 4 from 61 (in) to 76 (out) : -216.185 Transmembrane helix 5 from 80 (out) to 95 (in) : -85.16 Transmembrane helix 6 from 99 (in) to 114 (out) : -375.616 Transmembrane helix 7 from 125 (out) to 140 (in) : -1009.07 Transmembrane helix 8 from 144 (in) to 159 (out) : -275.381 Transmembrane helix 9 from 163 (out) to 178 (in) : -417.576 Transmembrane helix 10 from 182 (in) to 197 (out) : -166.331 Transmembrane helix 11 from 201 (out) to 216 (in) : -346.317 Transmembrane helix 12 from 220 (in) to 235 (out) : -473.528 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 23 (out) to 38 (in) : -528.737 Transmembrane helix 3 from 42 (in) to 57 (out) : -224.144 Transmembrane helix 4 from 61 (out) to 76 (in) : -223.015 Transmembrane helix 5 from 80 (in) to 95 (out) : -96.74 Transmembrane helix 6 from 99 (out) to 114 (in) : -363.784 Transmembrane helix 7 from 144 (in) to 159 (out) : -275.381 Transmembrane helix 8 from 163 (out) to 178 (in) : -417.576 Transmembrane helix 9 from 182 (in) to 197 (out) : -166.331 Transmembrane helix 10 from 201 (out) to 216 (in) : -346.317 Transmembrane helix 11 from 220 (in) to 235 (out) : -473.528 Re-entrant helix 1 from 118 (in) to 140 (in) : 1610 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -294.947 Transmembrane helix 2 from 30 (in) to 45 (out) : -448.669 Transmembrane helix 3 from 49 (out) to 64 (in) : -131.549 Transmembrane helix 4 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 5 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 6 from 144 (in) to 159 (out) : -275.381 Transmembrane helix 7 from 163 (out) to 178 (in) : -417.576 Transmembrane helix 8 from 182 (in) to 197 (out) : -166.331 Transmembrane helix 9 from 201 (out) to 216 (in) : -346.317 Transmembrane helix 10 from 220 (in) to 235 (out) : -473.528 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 39 (out) to 54 (in) : -263.906 Transmembrane helix 3 from 58 (in) to 73 (out) : -216.968 Transmembrane helix 4 from 77 (out) to 92 (in) : -75.437 Transmembrane helix 5 from 96 (in) to 111 (out) : -282.633 Transmembrane helix 6 from 145 (out) to 160 (in) : -261.999 Transmembrane helix 7 from 164 (in) to 179 (out) : -400.93 Transmembrane helix 8 from 183 (out) to 198 (in) : -143.937 Transmembrane helix 9 from 202 (in) to 217 (out) : -358.209 Re-entrant helix 1 from 23 (out) to 35 (out) : 490 Re-entrant helix 2 from 115 (out) to 141 (out) : 1890 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -294.947 Transmembrane helix 2 from 30 (in) to 45 (out) : -448.669 Transmembrane helix 3 from 49 (out) to 64 (in) : -131.549 Transmembrane helix 4 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 5 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 6 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 7 from 176 (out) to 191 (in) : -230.453 Transmembrane helix 8 from 195 (in) to 210 (out) : -236.568 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -306.053 Transmembrane helix 2 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 3 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 4 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 5 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 6 from 176 (out) to 191 (in) : -230.453 Transmembrane helix 7 from 195 (in) to 210 (out) : -236.568 Re-entrant helix 1 from 23 (out) to 44 (out) : 1120 Re-entrant helix 2 from 106 (in) to 140 (in) : 2450 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 2 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 3 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 4 from 150 (in) to 165 (out) : -218.771 Transmembrane helix 5 from 176 (out) to 191 (in) : -230.453 Transmembrane helix 6 from 195 (in) to 210 (out) : -236.568 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -136.951 Transmembrane helix 2 from 68 (out) to 83 (in) : -174.318 Transmembrane helix 3 from 87 (in) to 102 (out) : -117.15 Transmembrane helix 4 from 149 (out) to 164 (in) : -235.249 Transmembrane helix 5 from 187 (in) to 202 (out) : -126.89 Re-entrant helix 1 from 106 (out) to 140 (out) : 2450 Re-entrant helix 2 from 168 (in) to 183 (in) : 1120 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 48 (out) to 63 (in) : -130.726 Transmembrane helix 2 from 68 (in) to 83 (out) : -160.082 Transmembrane helix 3 from 87 (out) to 102 (in) : -102.25 Transmembrane helix 4 from 187 (in) to 202 (out) : -126.89 Re-entrant helix 1 from 106 (in) to 140 (in) : 2450 Re-entrant helix 2 from 141 (in) to 175 (in) : 2450 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 49 (in) to 64 (out) : -136.951 Transmembrane helix 2 from 77 (out) to 92 (in) : -75.437 Transmembrane helix 3 from 187 (in) to 202 (out) : -126.89 Re-entrant helix 1 from 96 (in) to 130 (in) : 2450 Re-entrant helix 2 from 131 (in) to 165 (in) : 2450 Re-entrant helix 3 from 166 (in) to 183 (in) : 1260 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 77 (out) to 92 (in) : -75.437 Transmembrane helix 2 from 187 (in) to 202 (out) : -126.89 Re-entrant helix 1 from 96 (in) to 130 (in) : 2450 Re-entrant helix 2 from 131 (in) to 165 (in) : 2450 Re-entrant helix 3 from 166 (in) to 183 (in) : 1260 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.077963 1 helix (-) : Score = -0.075437 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 77-92 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.075437