BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for composition-based statistics:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (71 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q0THK2 OriC-binding nucleoid-associated protein n=114 T...   103   2e-21
UniRef50_Q9F547 YdfA protein n=14 Tax=Enterobacteriaceae RepID=Q...    93   2e-18
UniRef50_P0ACE5 Hemolysin expression-modulating protein n=147 Ta...    86   3e-16
UniRef50_A7FCE9 Virulence modulating protein n=7 Tax=Enterobacte...    84   1e-15
UniRef50_Q5JBQ4 Hha n=76 Tax=Enterobacteriaceae RepID=Q5JBQ4_ECOLX     84   2e-15
UniRef50_B5RJT3 Haemolysin expression modulating protein n=3 Tax...    79   4e-14
UniRef50_B5EWE3 Conserved domain protein n=4 Tax=Enterobacteriac...    72   4e-12
UniRef50_B0EYN1 Hemolysin expression modulating protein n=13 Tax...    69   3e-11
UniRef50_Q326W4 Putative regulator n=1 Tax=Shigella dysenteriae ...    62   4e-09
UniRef50_B2U682 Conserved domain protein n=1 Tax=Escherichia col...    45   6e-04
UniRef50_Q47498 K12 plasmid DNA n=2 Tax=Enterobacteriaceae RepID...    40   0.023

>UniRef50_Q0THK2 OriC-binding nucleoid-associated protein n=114
          Tax=Enterobacteriaceae RepID=CNU_ECOL5
          Length = 71

 Score =  103 bits (257), Expect = 2e-21,   Method: Composition-based stats.
 Identities = 71/71 (100%), Positives = 71/71 (100%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG
Sbjct: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60

Query: 61 QVPKSVWHYVQ 71
          QVPKSVWHYVQ
Sbjct: 61 QVPKSVWHYVQ 71


>UniRef50_Q9F547 YdfA protein n=14 Tax=Enterobacteriaceae RepID=Q9F547_ECOLX
          Length = 75

 Score = 93.3 bits (230), Expect = 2e-18,   Method: Composition-based stats.
 Identities = 30/69 (43%), Positives = 43/69 (62%), Gaps = 4/69 (5%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQ 61
          T QD+L + R+ S+L++LEK+ +  N       EL +   AADHR AEL +  +L+D  +
Sbjct: 10 TKQDWLFQLRRCSTLDTLEKIIEK-NQNCLPPSELESFNAAADHRLAEL-TMNKLYD--K 65

Query: 62 VPKSVWHYV 70
          VP SVW YV
Sbjct: 66 VPPSVWKYV 74


>UniRef50_P0ACE5 Hemolysin expression-modulating protein n=147
          Tax=Enterobacteriaceae RepID=HHA_ECO57
          Length = 72

 Score = 86.3 bits (212), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 27/71 (38%), Positives = 47/71 (66%), Gaps = 4/71 (5%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          +T  DYL++ R+  ++++LE++ +   Y L+D  EL   Y AADHR AEL +  +L+D  
Sbjct: 6  LTKTDYLMRLRRCQTIDTLERVIEKNKYELSD-NELAVFYSAADHRLAEL-TMNKLYD-- 61

Query: 61 QVPKSVWHYVQ 71
          ++P SVW +++
Sbjct: 62 KIPSSVWKFIR 72


>UniRef50_A7FCE9 Virulence modulating protein n=7 Tax=Enterobacteriaceae
          RepID=A7FCE9_YERP3
          Length = 68

 Score = 84.0 bits (206), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 26/71 (36%), Positives = 47/71 (66%), Gaps = 4/71 (5%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          M   ++L++ R+ +++E+LE++ +   YTL+ D EL     AADHR AEL +  +L+D  
Sbjct: 2  MNKTEWLMRLRRCTTMETLEQIIERKKYTLSAD-ELETFNSAADHRLAEL-TMNKLYD-- 57

Query: 61 QVPKSVWHYVQ 71
          ++P SVW +++
Sbjct: 58 KIPASVWQHIK 68


>UniRef50_Q5JBQ4 Hha n=76 Tax=Enterobacteriaceae RepID=Q5JBQ4_ECOLX
          Length = 69

 Score = 83.7 bits (205), Expect = 2e-15,   Method: Composition-based stats.
 Identities = 27/70 (38%), Positives = 45/70 (64%), Gaps = 4/70 (5%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQ 61
          T Q++L + R+ SS+ +LEK+      +L  + E  +   AADHR AEL++ G+L+D  +
Sbjct: 4  TKQEWLYQLRRCSSVNTLEKIIHKNRDSLL-NSERESFNSAADHRLAELIT-GKLYD--R 59

Query: 62 VPKSVWHYVQ 71
          +PK +W YV+
Sbjct: 60 IPKEIWKYVR 69


>UniRef50_B5RJT3 Haemolysin expression modulating protein n=3 Tax=Klebsiella
          pneumoniae RepID=B5RJT3_KLEP3
          Length = 84

 Score = 79.0 bits (193), Expect = 4e-14,   Method: Composition-based stats.
 Identities = 27/72 (37%), Positives = 45/72 (62%), Gaps = 7/72 (9%)

Query: 3  VQDYLLKFRKISSLESLEKLYD----HLNYTLTDDQELINMYRAADHRRAELVSGGRLFD 58
           + YLLKFRK S+ E+L+K+++     LN    D  ++ ++  A DHRRAE+    +++D
Sbjct: 12 RETYLLKFRKCSTTETLDKVFERILDKLNDEGGDINKITSLNGAYDHRRAEIY-MKKIYD 70

Query: 59 LGQVPKSVWHYV 70
            ++P SVWH +
Sbjct: 71 --KIPASVWHLI 80


>UniRef50_B5EWE3 Conserved domain protein n=4 Tax=Enterobacteriaceae
          RepID=B5EWE3_SALA4
          Length = 67

 Score = 72.5 bits (176), Expect = 4e-12,   Method: Composition-based stats.
 Identities = 29/71 (40%), Positives = 47/71 (66%), Gaps = 4/71 (5%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          M   +YLLKFR+ ++ E+LEK+ +H+   ++  ++      AADHRRAEL +  ++FD  
Sbjct: 1  MDKLEYLLKFRRSNNDETLEKVVEHMEPKISK-EDYNAFQAAADHRRAEL-ACHKIFD-- 56

Query: 61 QVPKSVWHYVQ 71
          +VPKS W +V+
Sbjct: 57 KVPKSAWKFVK 67


>UniRef50_B0EYN1 Hemolysin expression modulating protein n=13
          Tax=Enterobacteriaceae RepID=B0EYN1_ECOLX
          Length = 71

 Score = 69.4 bits (168), Expect = 3e-11,   Method: Composition-based stats.
 Identities = 25/53 (47%), Positives = 31/53 (58%), Gaps = 4/53 (7%)

Query: 19 LEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQVPKSVWHYVQ 71
          LEK+ +   Y L    E    Y AADHRRAELV   +L+D  +VP  VW YV+
Sbjct: 23 LEKVIEINRYKL-PLSESEAFYSAADHRRAELV-MNKLYD--KVPSGVWKYVR 71


>UniRef50_Q326W4 Putative regulator n=1 Tax=Shigella dysenteriae Sd197
          RepID=Q326W4_SHIDS
          Length = 62

 Score = 62.5 bits (150), Expect = 4e-09,   Method: Composition-based stats.
 Identities = 19/51 (37%), Positives = 32/51 (62%), Gaps = 4/51 (7%)

Query: 21 KLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQVPKSVWHYVQ 71
          K+      +L+ + E  +   AADHR AEL++ G+L+D  ++PK +W YV+
Sbjct: 16 KIIHKNRDSLS-NSERESFNSAADHRLAELIT-GKLYD--RIPKEIWKYVR 62


>UniRef50_B2U682 Conserved domain protein n=1 Tax=Escherichia coli 53638
          RepID=B2U682_ECOLX
          Length = 39

 Score = 45.1 bits (105), Expect = 6e-04,   Method: Composition-based stats.
 Identities = 10/30 (33%), Positives = 19/30 (63%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLT 31
          T Q++L + R+ SS+ +LEK+      +L+
Sbjct: 4  TKQEWLYQLRRCSSVNTLEKIIHKNRDSLS 33


>UniRef50_Q47498 K12 plasmid DNA n=2 Tax=Enterobacteriaceae RepID=Q47498_ECOLX
          Length = 70

 Score = 40.1 bits (92), Expect = 0.023,   Method: Composition-based stats.
 Identities = 20/67 (29%), Positives = 37/67 (55%), Gaps = 4/67 (5%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQ 61
          + +D+L   R+  + +SL+   D     L    E + +  AA+HRR E+ + G+LFD  +
Sbjct: 8  SAEDWLHTVRRFMNEDSLDTYVDSKRDVL-PATEFMRLLTAAEHRRVEIRT-GKLFD--K 63

Query: 62 VPKSVWH 68
          +PK ++ 
Sbjct: 64 IPKGLFR 70


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q0THK2 OriC-binding nucleoid-associated protein n=114 T...   102   4e-21
UniRef50_Q9F547 YdfA protein n=14 Tax=Enterobacteriaceae RepID=Q...    94   1e-18
UniRef50_P0ACE5 Hemolysin expression-modulating protein n=147 Ta...    86   3e-16
UniRef50_A7FCE9 Virulence modulating protein n=7 Tax=Enterobacte...    84   1e-15
UniRef50_Q5JBQ4 Hha n=76 Tax=Enterobacteriaceae RepID=Q5JBQ4_ECOLX     84   1e-15
UniRef50_B5EWE3 Conserved domain protein n=4 Tax=Enterobacteriac...    78   8e-14
UniRef50_B5RJT3 Haemolysin expression modulating protein n=3 Tax...    77   1e-13
UniRef50_B0EYN1 Hemolysin expression modulating protein n=13 Tax...    69   4e-11
UniRef50_Q326W4 Putative regulator n=1 Tax=Shigella dysenteriae ...    62   4e-09
UniRef50_B2U682 Conserved domain protein n=1 Tax=Escherichia col...    46   3e-04

Sequences not found previously or not previously below threshold:

UniRef50_Q47498 K12 plasmid DNA n=2 Tax=Enterobacteriaceae RepID...    40   0.025


CONVERGED!
>UniRef50_Q0THK2 OriC-binding nucleoid-associated protein n=114
          Tax=Enterobacteriaceae RepID=CNU_ECOL5
          Length = 71

 Score =  102 bits (254), Expect = 4e-21,   Method: Composition-based stats.
 Identities = 71/71 (100%), Positives = 71/71 (100%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG
Sbjct: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60

Query: 61 QVPKSVWHYVQ 71
          QVPKSVWHYVQ
Sbjct: 61 QVPKSVWHYVQ 71


>UniRef50_Q9F547 YdfA protein n=14 Tax=Enterobacteriaceae RepID=Q9F547_ECOLX
          Length = 75

 Score = 93.6 bits (231), Expect = 1e-18,   Method: Composition-based stats.
 Identities = 30/69 (43%), Positives = 43/69 (62%), Gaps = 4/69 (5%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQ 61
          T QD+L + R+ S+L++LEK+ +  N       EL +   AADHR AEL +  +L+D  +
Sbjct: 10 TKQDWLFQLRRCSTLDTLEKIIEK-NQNCLPPSELESFNAAADHRLAEL-TMNKLYD--K 65

Query: 62 VPKSVWHYV 70
          VP SVW YV
Sbjct: 66 VPPSVWKYV 74


>UniRef50_P0ACE5 Hemolysin expression-modulating protein n=147
          Tax=Enterobacteriaceae RepID=HHA_ECO57
          Length = 72

 Score = 85.9 bits (211), Expect = 3e-16,   Method: Composition-based stats.
 Identities = 27/71 (38%), Positives = 47/71 (66%), Gaps = 4/71 (5%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          +T  DYL++ R+  ++++LE++ +   Y L+D  EL   Y AADHR AEL +  +L+D  
Sbjct: 6  LTKTDYLMRLRRCQTIDTLERVIEKNKYELSD-NELAVFYSAADHRLAEL-TMNKLYD-- 61

Query: 61 QVPKSVWHYVQ 71
          ++P SVW +++
Sbjct: 62 KIPSSVWKFIR 72


>UniRef50_A7FCE9 Virulence modulating protein n=7 Tax=Enterobacteriaceae
          RepID=A7FCE9_YERP3
          Length = 68

 Score = 84.0 bits (206), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 26/71 (36%), Positives = 47/71 (66%), Gaps = 4/71 (5%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          M   ++L++ R+ +++E+LE++ +   YTL+ D EL     AADHR AEL +  +L+D  
Sbjct: 2  MNKTEWLMRLRRCTTMETLEQIIERKKYTLSAD-ELETFNSAADHRLAEL-TMNKLYD-- 57

Query: 61 QVPKSVWHYVQ 71
          ++P SVW +++
Sbjct: 58 KIPASVWQHIK 68


>UniRef50_Q5JBQ4 Hha n=76 Tax=Enterobacteriaceae RepID=Q5JBQ4_ECOLX
          Length = 69

 Score = 84.0 bits (206), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 27/70 (38%), Positives = 45/70 (64%), Gaps = 4/70 (5%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQ 61
          T Q++L + R+ SS+ +LEK+      +L  + E  +   AADHR AEL++ G+L+D  +
Sbjct: 4  TKQEWLYQLRRCSSVNTLEKIIHKNRDSLL-NSERESFNSAADHRLAELIT-GKLYD--R 59

Query: 62 VPKSVWHYVQ 71
          +PK +W YV+
Sbjct: 60 IPKEIWKYVR 69


>UniRef50_B5EWE3 Conserved domain protein n=4 Tax=Enterobacteriaceae
          RepID=B5EWE3_SALA4
          Length = 67

 Score = 77.9 bits (190), Expect = 8e-14,   Method: Composition-based stats.
 Identities = 29/71 (40%), Positives = 47/71 (66%), Gaps = 4/71 (5%)

Query: 1  MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLG 60
          M   +YLLKFR+ ++ E+LEK+ +H+   ++  ++      AADHRRAEL +  ++FD  
Sbjct: 1  MDKLEYLLKFRRSNNDETLEKVVEHMEPKISK-EDYNAFQAAADHRRAEL-ACHKIFD-- 56

Query: 61 QVPKSVWHYVQ 71
          +VPKS W +V+
Sbjct: 57 KVPKSAWKFVK 67


>UniRef50_B5RJT3 Haemolysin expression modulating protein n=3 Tax=Klebsiella
          pneumoniae RepID=B5RJT3_KLEP3
          Length = 84

 Score = 77.5 bits (189), Expect = 1e-13,   Method: Composition-based stats.
 Identities = 27/72 (37%), Positives = 45/72 (62%), Gaps = 7/72 (9%)

Query: 3  VQDYLLKFRKISSLESLEKLYD----HLNYTLTDDQELINMYRAADHRRAELVSGGRLFD 58
           + YLLKFRK S+ E+L+K+++     LN    D  ++ ++  A DHRRAE+    +++D
Sbjct: 12 RETYLLKFRKCSTTETLDKVFERILDKLNDEGGDINKITSLNGAYDHRRAEIY-MKKIYD 70

Query: 59 LGQVPKSVWHYV 70
            ++P SVWH +
Sbjct: 71 --KIPASVWHLI 80


>UniRef50_B0EYN1 Hemolysin expression modulating protein n=13
          Tax=Enterobacteriaceae RepID=B0EYN1_ECOLX
          Length = 71

 Score = 69.0 bits (167), Expect = 4e-11,   Method: Composition-based stats.
 Identities = 25/53 (47%), Positives = 31/53 (58%), Gaps = 4/53 (7%)

Query: 19 LEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQVPKSVWHYVQ 71
          LEK+ +   Y L    E    Y AADHRRAELV   +L+D  +VP  VW YV+
Sbjct: 23 LEKVIEINRYKL-PLSESEAFYSAADHRRAELV-MNKLYD--KVPSGVWKYVR 71


>UniRef50_Q326W4 Putative regulator n=1 Tax=Shigella dysenteriae Sd197
          RepID=Q326W4_SHIDS
          Length = 62

 Score = 62.4 bits (150), Expect = 4e-09,   Method: Composition-based stats.
 Identities = 19/51 (37%), Positives = 32/51 (62%), Gaps = 4/51 (7%)

Query: 21 KLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQVPKSVWHYVQ 71
          K+      +L+ + E  +   AADHR AEL++ G+L+D  ++PK +W YV+
Sbjct: 16 KIIHKNRDSLS-NSERESFNSAADHRLAELIT-GKLYD--RIPKEIWKYVR 62


>UniRef50_B2U682 Conserved domain protein n=1 Tax=Escherichia coli 53638
          RepID=B2U682_ECOLX
          Length = 39

 Score = 46.3 bits (108), Expect = 3e-04,   Method: Composition-based stats.
 Identities = 10/30 (33%), Positives = 19/30 (63%)

Query: 2  TVQDYLLKFRKISSLESLEKLYDHLNYTLT 31
          T Q++L + R+ SS+ +LEK+      +L+
Sbjct: 4  TKQEWLYQLRRCSSVNTLEKIIHKNRDSLS 33


>UniRef50_Q47498 K12 plasmid DNA n=2 Tax=Enterobacteriaceae RepID=Q47498_ECOLX
          Length = 70

 Score = 39.7 bits (91), Expect = 0.025,   Method: Composition-based stats.
 Identities = 20/65 (30%), Positives = 36/65 (55%), Gaps = 4/65 (6%)

Query: 4  QDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAADHRRAELVSGGRLFDLGQVP 63
          +D+L   R+  + +SL+   D     L    E + +  AA+HRR E+ + G+LFD  ++P
Sbjct: 10 EDWLHTVRRFMNEDSLDTYVDSKRDVL-PATEFMRLLTAAEHRRVEIRT-GKLFD--KIP 65

Query: 64 KSVWH 68
          K ++ 
Sbjct: 66 KGLFR 70


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.309    0.129    0.353 

Lambda     K      H
   0.267   0.0394    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 227,182,871
Number of Sequences: 3077464
Number of extensions: 4512639
Number of successful extensions: 17063
Number of sequences better than 1.0e-01: 11
Number of HSP's better than  0.1 without gapping: 18
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 17014
Number of HSP's gapped (non-prelim): 22
length of query: 71
length of database: 1,040,396,356
effective HSP length: 43
effective length of query: 28
effective length of database: 908,065,404
effective search space: 25425831312
effective search space used: 25425831312
T: 11
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.3 bits)
S2: 87 (38.2 bits)
