Processing 1 helix: Transmembrane helix 1 from 153 (in) to 183 (out) : 3680.03 Score = 3.650911 Processing 1 helix: Transmembrane helix 1 from 153 (out) to 183 (in) : 3671.17 Score = 3.700289 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 20 (out) : 516.305 Transmembrane helix 2 from 26 (out) to 41 (in) : 571.101 Transmembrane helix 3 from 45 (in) to 60 (out) : -180.466 Transmembrane helix 4 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 5 from 91 (in) to 106 (out) : 1396.46 Transmembrane helix 6 from 110 (out) to 125 (in) : 645.256 Transmembrane helix 7 from 129 (in) to 144 (out) : 177.445 Transmembrane helix 8 from 148 (out) to 163 (in) : 2188.85 Transmembrane helix 9 from 167 (in) to 182 (out) : 1661.27 Transmembrane helix 10 from 186 (out) to 201 (in) : 414.771 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : 540.666 Transmembrane helix 2 from 26 (in) to 41 (out) : 563.499 Transmembrane helix 3 from 45 (out) to 60 (in) : -189.534 Transmembrane helix 4 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 5 from 91 (out) to 106 (in) : 1363.14 Transmembrane helix 6 from 110 (in) to 125 (out) : 619.844 Transmembrane helix 7 from 129 (out) to 144 (in) : 180.655 Transmembrane helix 8 from 148 (in) to 164 (out) : 2249.08 Transmembrane helix 9 from 172 (out) to 192 (in) : 2763.39 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 20 (out) : 516.305 Transmembrane helix 2 from 26 (out) to 41 (in) : 571.101 Transmembrane helix 3 from 45 (in) to 60 (out) : -180.466 Transmembrane helix 4 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 5 from 91 (in) to 106 (out) : 1396.46 Transmembrane helix 6 from 116 (out) to 133 (in) : 1218.4 Transmembrane helix 7 from 148 (in) to 164 (out) : 2249.08 Transmembrane helix 8 from 172 (out) to 192 (in) : 2763.39 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : 540.666 Transmembrane helix 2 from 30 (in) to 50 (out) : 802.53 Transmembrane helix 3 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 4 from 91 (in) to 106 (out) : 1396.46 Transmembrane helix 5 from 116 (out) to 133 (in) : 1218.4 Transmembrane helix 6 from 148 (in) to 164 (out) : 2249.08 Transmembrane helix 7 from 172 (out) to 192 (in) : 2763.39 Score = 12.230009 Processing 6 helices: Transmembrane helix 1 from 12 (in) to 42 (out) : 889.829 Transmembrane helix 2 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 3 from 91 (in) to 106 (out) : 1396.46 Transmembrane helix 4 from 116 (out) to 133 (in) : 1218.4 Transmembrane helix 5 from 148 (in) to 164 (out) : 2249.08 Transmembrane helix 6 from 172 (out) to 192 (in) : 2763.39 Score = 11.741044 Processing 5 helices: Transmembrane helix 1 from 12 (out) to 42 (in) : 920.171 Transmembrane helix 2 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 3 from 93 (out) to 123 (in) : 1760.71 Transmembrane helix 4 from 148 (in) to 164 (out) : 2249.08 Transmembrane helix 5 from 172 (out) to 192 (in) : 2763.39 Score = 10.884871 Processing 4 helices: Transmembrane helix 1 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 2 from 93 (out) to 123 (in) : 1760.71 Transmembrane helix 3 from 148 (in) to 164 (out) : 2249.08 Transmembrane helix 4 from 172 (out) to 192 (in) : 2763.39 Score = 9.957193 Processing 3 helices: Transmembrane helix 1 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 2 from 93 (in) to 123 (out) : 1788.39 Transmembrane helix 3 from 153 (out) to 183 (in) : 3671.17 Score = 8.607219 Processing 2 helices: Transmembrane helix 1 from 66 (in) to 96 (out) : 3235.77 Transmembrane helix 2 from 153 (out) to 183 (in) : 3671.17 Score = 6.920503 Processing 10 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : 540.666 Transmembrane helix 2 from 26 (in) to 41 (out) : 563.499 Transmembrane helix 3 from 45 (out) to 60 (in) : -189.534 Transmembrane helix 4 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 5 from 91 (out) to 106 (in) : 1363.14 Transmembrane helix 6 from 110 (in) to 125 (out) : 619.844 Transmembrane helix 7 from 129 (out) to 144 (in) : 180.655 Transmembrane helix 8 from 148 (in) to 163 (out) : 2213.75 Transmembrane helix 9 from 167 (out) to 182 (in) : 1681.93 Transmembrane helix 10 from 186 (in) to 201 (out) : 422.329 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 20 (out) : 516.305 Transmembrane helix 2 from 26 (out) to 41 (in) : 571.101 Transmembrane helix 3 from 45 (in) to 60 (out) : -180.466 Transmembrane helix 4 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 5 from 91 (in) to 106 (out) : 1396.46 Transmembrane helix 6 from 111 (out) to 126 (in) : 738.119 Transmembrane helix 7 from 130 (in) to 145 (out) : 84.575 Transmembrane helix 8 from 149 (out) to 164 (in) : 2216.81 Transmembrane helix 9 from 172 (in) to 192 (out) : 2712.61 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : 540.666 Transmembrane helix 2 from 30 (in) to 50 (out) : 802.53 Transmembrane helix 3 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 4 from 91 (in) to 106 (out) : 1396.46 Transmembrane helix 5 from 111 (out) to 126 (in) : 738.119 Transmembrane helix 6 from 130 (in) to 145 (out) : 84.575 Transmembrane helix 7 from 149 (out) to 164 (in) : 2216.81 Transmembrane helix 8 from 172 (in) to 192 (out) : 2712.61 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 20 (out) : 516.305 Transmembrane helix 2 from 30 (out) to 50 (in) : 782.37 Transmembrane helix 3 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 4 from 91 (out) to 106 (in) : 1363.14 Transmembrane helix 5 from 116 (in) to 133 (out) : 1189 Transmembrane helix 6 from 148 (out) to 164 (in) : 2219.12 Transmembrane helix 7 from 172 (in) to 192 (out) : 2712.61 Score = 11.801198 Processing 6 helices: Transmembrane helix 1 from 12 (out) to 42 (in) : 920.171 Transmembrane helix 2 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 3 from 91 (out) to 106 (in) : 1363.14 Transmembrane helix 4 from 116 (in) to 133 (out) : 1189 Transmembrane helix 5 from 148 (out) to 164 (in) : 2219.12 Transmembrane helix 6 from 172 (in) to 192 (out) : 2712.61 Score = 11.456156 Processing 5 helices: Transmembrane helix 1 from 12 (in) to 42 (out) : 889.829 Transmembrane helix 2 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 3 from 93 (in) to 123 (out) : 1788.39 Transmembrane helix 4 from 148 (out) to 164 (in) : 2219.12 Transmembrane helix 5 from 172 (in) to 192 (out) : 2712.61 Score = 10.694428 Processing 4 helices: Transmembrane helix 1 from 64 (out) to 79 (in) : 3143.19 Transmembrane helix 2 from 93 (in) to 123 (out) : 1788.39 Transmembrane helix 3 from 148 (out) to 164 (in) : 2219.12 Transmembrane helix 4 from 172 (in) to 192 (out) : 2712.61 Score = 9.812106 Processing 3 helices: Transmembrane helix 1 from 64 (in) to 79 (out) : 3132.81 Transmembrane helix 2 from 93 (out) to 123 (in) : 1760.71 Transmembrane helix 3 from 153 (in) to 183 (out) : 3680.03 Score = 8.569081 Processing 2 helices: Transmembrane helix 1 from 66 (out) to 96 (in) : 3264.13 Transmembrane helix 2 from 153 (in) to 183 (out) : 3680.03 Score = 6.930599 Summary of topology analysis: 1 helix (+) : Score = 3.65091 1 helix (-) : Score = 3.70029 2 helices (+) : Score = 6.9205 2 helices (-) : Score = 6.9306 3 helices (+) : Score = 8.56908 3 helices (-) : Score = 8.60722 4 helices (+) : Score = 9.95719 4 helices (-) : Score = 9.81211 5 helices (+) : Score = 10.6944 5 helices (-) : Score = 10.8849 6 helices (+) : Score = 11.741 6 helices (-) : Score = 11.4562 7 helices (+) : Score = 11.8012 7 helices (-) : Score = 12.23 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 5-20,30-50,64-79,91-106,116-133,148-164,172-192 Re-entrant helices: Not detected. Helix count: 7 N-terminal: out Score: 12.23