BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (196 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_P28697 Putative uncharacterized protein htgA n=10 Tax=E...   375   e-103

>UniRef50_P28697 Putative uncharacterized protein htgA n=10 Tax=Enterobacteriaceae
           RepID=HTGA_ECOLI
          Length = 196

 Score =  375 bits (962), Expect = e-103,   Method: Compositional matrix adjust.
 Identities = 196/196 (100%), Positives = 196/196 (100%)

Query: 1   MTALTPFSAAPTGPPSPAPRSKPCPSTLIAAWVRKMRVSWLESKCDTPFANNLSFISSGS 60
           MTALTPFSAAPTGPPSPAPRSKPCPSTLIAAWVRKMRVSWLESKCDTPFANNLSFISSGS
Sbjct: 1   MTALTPFSAAPTGPPSPAPRSKPCPSTLIAAWVRKMRVSWLESKCDTPFANNLSFISSGS 60

Query: 61  SSSSSFTLASTACRNSCLCSSSIFFQVLRRNCSSNCCSISNVDISLSAFSFNRFETSSKM 120
           SSSSSFTLASTACRNSCLCSSSIFFQVLRRNCSSNCCSISNVDISLSAFSFNRFETSSKM
Sbjct: 61  SSSSSFTLASTACRNSCLCSSSIFFQVLRRNCSSNCCSISNVDISLSAFSFNRFETSSKM 120

Query: 121 ARYNLPCPRSLLAILSPPKCCNSPAISCQLRRCCSGCPSIDLNSSLRISTLERRVLPFSL 180
           ARYNLPCPRSLLAILSPPKCCNSPAISCQLRRCCSGCPSIDLNSSLRISTLERRVLPFSL
Sbjct: 121 ARYNLPCPRSLLAILSPPKCCNSPAISCQLRRCCSGCPSIDLNSSLRISTLERRVLPFSL 180

Query: 181 WVSNRAKFANCSSLQC 196
           WVSNRAKFANCSSLQC
Sbjct: 181 WVSNRAKFANCSSLQC 196


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_P28697 Putative uncharacterized protein htgA n=10 Tax=E...   291   8e-78

Sequences not found previously or not previously below threshold:


CONVERGED!
>UniRef50_P28697 Putative uncharacterized protein htgA n=10 Tax=Enterobacteriaceae
           RepID=HTGA_ECOLI
          Length = 196

 Score =  291 bits (745), Expect = 8e-78,   Method: Composition-based stats.
 Identities = 196/196 (100%), Positives = 196/196 (100%)

Query: 1   MTALTPFSAAPTGPPSPAPRSKPCPSTLIAAWVRKMRVSWLESKCDTPFANNLSFISSGS 60
           MTALTPFSAAPTGPPSPAPRSKPCPSTLIAAWVRKMRVSWLESKCDTPFANNLSFISSGS
Sbjct: 1   MTALTPFSAAPTGPPSPAPRSKPCPSTLIAAWVRKMRVSWLESKCDTPFANNLSFISSGS 60

Query: 61  SSSSSFTLASTACRNSCLCSSSIFFQVLRRNCSSNCCSISNVDISLSAFSFNRFETSSKM 120
           SSSSSFTLASTACRNSCLCSSSIFFQVLRRNCSSNCCSISNVDISLSAFSFNRFETSSKM
Sbjct: 61  SSSSSFTLASTACRNSCLCSSSIFFQVLRRNCSSNCCSISNVDISLSAFSFNRFETSSKM 120

Query: 121 ARYNLPCPRSLLAILSPPKCCNSPAISCQLRRCCSGCPSIDLNSSLRISTLERRVLPFSL 180
           ARYNLPCPRSLLAILSPPKCCNSPAISCQLRRCCSGCPSIDLNSSLRISTLERRVLPFSL
Sbjct: 121 ARYNLPCPRSLLAILSPPKCCNSPAISCQLRRCCSGCPSIDLNSSLRISTLERRVLPFSL 180

Query: 181 WVSNRAKFANCSSLQC 196
           WVSNRAKFANCSSLQC
Sbjct: 181 WVSNRAKFANCSSLQC 196


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.322    0.128    0.413 

Lambda     K      H
   0.267   0.0393    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 769,809,613
Number of Sequences: 3077464
Number of extensions: 29477780
Number of successful extensions: 269802
Number of sequences better than 1.0e-01: 9
Number of HSP's better than  0.1 without gapping: 4
Number of HSP's successfully gapped in prelim test: 14
Number of HSP's that attempted gapping in prelim test: 268958
Number of HSP's gapped (non-prelim): 512
length of query: 196
length of database: 1,040,396,356
effective HSP length: 122
effective length of query: 74
effective length of database: 664,945,748
effective search space: 49205985352
effective search space used: 49205985352
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 89 (39.0 bits)