BLASTP 2.2.22 [Sep-27-2009]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= batch____
         (46 letters)

Database: uniref50.fasta 
           3,077,464 sequences; 1,040,396,356 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q47272 Uncharacterized protein ylcG in lambdoid DLP12 p...    97   2e-19
UniRef50_B5Y0G4 Putative uncharacterized protein n=2 Tax=Klebsie...    42   0.008
UniRef50_C9XDJ8 Predicted prophage protein n=11 Tax=Enterobacter...    40   0.027

>UniRef50_Q47272 Uncharacterized protein ylcG in lambdoid DLP12 prophage region
          n=36 Tax=root RepID=YLCG_ECOLI
          Length = 46

 Score = 96.7 bits (239), Expect = 2e-19,   Method: Compositional matrix adjust.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 1  MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAGV 46
          MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAGV
Sbjct: 1  MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAGV 46


>UniRef50_B5Y0G4 Putative uncharacterized protein n=2 Tax=Klebsiella pneumoniae
          RepID=B5Y0G4_KLEP3
          Length = 46

 Score = 41.6 bits (96), Expect = 0.008,   Method: Compositional matrix adjust.
 Identities = 19/40 (47%), Positives = 25/40 (62%)

Query: 6  NMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAG 45
          +  E +R RW +LRL R  G+VL DYRILKN+ +     G
Sbjct: 4  DAIERIRDRWQKLRLCRHRGTVLVDYRILKNFVRIYQTLG 43


>UniRef50_C9XDJ8 Predicted prophage protein n=11 Tax=Enterobacteriaceae
          RepID=C9XDJ8_SALTD
          Length = 48

 Score = 39.7 bits (91), Expect = 0.027,   Method: Compositional matrix adjust.
 Identities = 18/34 (52%), Positives = 24/34 (70%)

Query: 6  NMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAK 39
           + E+LR RW RLR+YR+ GS    YRIL+NY +
Sbjct: 4  ELIEILRMRWLRLRIYRYRGSFPVAYRILRNYVR 37


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

UniRef50_Q47272 Uncharacterized protein ylcG in lambdoid DLP12 p...    84   1e-15

Sequences not found previously or not previously below threshold:

UniRef50_B5Y0G4 Putative uncharacterized protein n=2 Tax=Klebsie...    39   0.056


CONVERGED!
>UniRef50_Q47272 Uncharacterized protein ylcG in lambdoid DLP12 prophage region
          n=36 Tax=root RepID=YLCG_ECOLI
          Length = 46

 Score = 83.9 bits (206), Expect = 1e-15,   Method: Composition-based stats.
 Identities = 46/46 (100%), Positives = 46/46 (100%)

Query: 1  MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAGV 46
          MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAGV
Sbjct: 1  MMFEFNMAELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAGV 46


>UniRef50_B5Y0G4 Putative uncharacterized protein n=2 Tax=Klebsiella pneumoniae
          RepID=B5Y0G4_KLEP3
          Length = 46

 Score = 38.8 bits (89), Expect = 0.056,   Method: Composition-based stats.
 Identities = 19/37 (51%), Positives = 24/37 (64%)

Query: 9  ELLRHRWGRLRLYRFPGSVLTDYRILKNYAKTLTGAG 45
          E +R RW +LRL R  G+VL DYRILKN+ +     G
Sbjct: 7  ERIRDRWQKLRLCRHRGTVLVDYRILKNFVRIYQTLG 43


  Database: uniref50.fasta
    Posted date:  Mar 8, 2010 10:38 AM
  Number of letters in database: 1,040,396,356
  Number of sequences in database:  3,077,464
  
Lambda     K      H
   0.331    0.144    0.453 

Lambda     K      H
   0.267   0.0467    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 230,481,436
Number of Sequences: 3077464
Number of extensions: 6771665
Number of successful extensions: 18984
Number of sequences better than 1.0e-01: 3
Number of HSP's better than  0.1 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 18978
Number of HSP's gapped (non-prelim): 6
length of query: 46
length of database: 1,040,396,356
effective HSP length: 20
effective length of query: 26
effective length of database: 978,847,076
effective search space: 25450023976
effective search space used: 25450023976
T: 11
A: 40
X1: 16 ( 7.6 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 87 (37.9 bits)