Processing 1 helix: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Score = -0.299825 Processing 1 helix: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Score = -0.130375 Processing 12 helices: Transmembrane helix 1 from 5 (in) to 20 (out) : -25352.5 Transmembrane helix 2 from 24 (out) to 39 (in) : -744.155 Transmembrane helix 3 from 43 (in) to 58 (out) : -1290.9 Transmembrane helix 4 from 62 (out) to 77 (in) : -1655.18 Transmembrane helix 5 from 81 (in) to 96 (out) : -1064.71 Transmembrane helix 6 from 100 (out) to 115 (in) : -1232.36 Transmembrane helix 7 from 119 (in) to 134 (out) : -1070.73 Transmembrane helix 8 from 138 (out) to 153 (in) : -974.448 Transmembrane helix 9 from 157 (in) to 172 (out) : -1015.58 Transmembrane helix 10 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 11 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 12 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 24 (out) to 39 (in) : -744.155 Transmembrane helix 2 from 43 (in) to 58 (out) : -1290.9 Transmembrane helix 3 from 62 (out) to 77 (in) : -1655.18 Transmembrane helix 4 from 81 (in) to 96 (out) : -1064.71 Transmembrane helix 5 from 100 (out) to 115 (in) : -1232.36 Transmembrane helix 6 from 119 (in) to 134 (out) : -1070.73 Transmembrane helix 7 from 138 (out) to 153 (in) : -974.448 Transmembrane helix 8 from 157 (in) to 172 (out) : -1015.58 Transmembrane helix 9 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 10 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 11 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 45 (out) to 60 (in) : -1404.07 Transmembrane helix 3 from 78 (in) to 93 (out) : -1086.12 Transmembrane helix 4 from 97 (out) to 112 (in) : -1215.74 Transmembrane helix 5 from 116 (in) to 131 (out) : -1177.12 Transmembrane helix 6 from 135 (out) to 150 (in) : -818.909 Transmembrane helix 7 from 157 (in) to 172 (out) : -1015.58 Transmembrane helix 8 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 9 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 10 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 45 (in) to 60 (out) : -1399.23 Transmembrane helix 3 from 84 (out) to 99 (in) : -1016.24 Transmembrane helix 4 from 108 (in) to 123 (out) : -1180.38 Transmembrane helix 5 from 130 (out) to 145 (in) : -689.619 Transmembrane helix 6 from 157 (in) to 172 (out) : -1015.58 Transmembrane helix 7 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 8 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 9 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 84 (out) to 99 (in) : -1016.24 Transmembrane helix 3 from 108 (in) to 123 (out) : -1180.38 Transmembrane helix 4 from 130 (out) to 145 (in) : -689.619 Transmembrane helix 5 from 157 (in) to 172 (out) : -1015.58 Transmembrane helix 6 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 7 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 8 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 84 (in) to 99 (out) : -1034.66 Transmembrane helix 3 from 130 (out) to 145 (in) : -689.619 Transmembrane helix 4 from 157 (in) to 172 (out) : -1015.58 Transmembrane helix 5 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 6 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 7 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 84 (out) to 99 (in) : -1016.24 Transmembrane helix 3 from 130 (in) to 145 (out) : -689.481 Transmembrane helix 4 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 5 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 6 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 130 (in) to 145 (out) : -689.481 Transmembrane helix 3 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 4 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 5 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 3 from 195 (in) to 210 (out) : -656.509 Transmembrane helix 4 from 214 (out) to 229 (in) : -403.525 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 3 from 212 (out) to 227 (in) : -395.891 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 212 (out) to 227 (in) : -395.891 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 5 (out) to 20 (in) : -25351.5 Transmembrane helix 2 from 24 (in) to 39 (out) : -754.845 Transmembrane helix 3 from 43 (out) to 58 (in) : -1295.7 Transmembrane helix 4 from 62 (in) to 77 (out) : -1644.02 Transmembrane helix 5 from 81 (out) to 96 (in) : -1048.89 Transmembrane helix 6 from 100 (in) to 115 (out) : -1229.54 Transmembrane helix 7 from 119 (out) to 134 (in) : -1073.77 Transmembrane helix 8 from 138 (in) to 153 (out) : -978.752 Transmembrane helix 9 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 10 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 11 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 12 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 24 (in) to 39 (out) : -754.845 Transmembrane helix 2 from 43 (out) to 58 (in) : -1295.7 Transmembrane helix 3 from 62 (in) to 77 (out) : -1644.02 Transmembrane helix 4 from 81 (out) to 96 (in) : -1048.89 Transmembrane helix 5 from 100 (in) to 115 (out) : -1229.54 Transmembrane helix 6 from 119 (out) to 134 (in) : -1073.77 Transmembrane helix 7 from 138 (in) to 153 (out) : -978.752 Transmembrane helix 8 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 9 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 10 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 11 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 45 (in) to 60 (out) : -1399.23 Transmembrane helix 3 from 79 (out) to 94 (in) : -1059.03 Transmembrane helix 4 from 98 (in) to 113 (out) : -1202.87 Transmembrane helix 5 from 117 (out) to 132 (in) : -1150.25 Transmembrane helix 6 from 136 (in) to 151 (out) : -869.975 Transmembrane helix 7 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 8 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 9 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 10 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 79 (out) to 94 (in) : -1059.03 Transmembrane helix 3 from 98 (in) to 113 (out) : -1202.87 Transmembrane helix 4 from 117 (out) to 132 (in) : -1150.25 Transmembrane helix 5 from 136 (in) to 151 (out) : -869.975 Transmembrane helix 6 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 7 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 8 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 9 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 84 (in) to 99 (out) : -1034.66 Transmembrane helix 3 from 110 (out) to 125 (in) : -1181.72 Transmembrane helix 4 from 130 (in) to 145 (out) : -689.481 Transmembrane helix 5 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 6 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 7 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 8 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 84 (out) to 99 (in) : -1016.24 Transmembrane helix 3 from 130 (in) to 145 (out) : -689.481 Transmembrane helix 4 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 5 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 6 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 7 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 130 (in) to 145 (out) : -689.481 Transmembrane helix 3 from 157 (out) to 172 (in) : -1016.72 Transmembrane helix 4 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 5 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 6 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 130 (out) to 145 (in) : -689.619 Transmembrane helix 3 from 175 (in) to 190 (out) : -589.782 Transmembrane helix 4 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 5 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 176 (in) to 191 (out) : -587.9 Transmembrane helix 3 from 195 (out) to 210 (in) : -652.491 Transmembrane helix 4 from 214 (in) to 229 (out) : -414.275 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 26 (in) to 41 (out) : -219.206 Transmembrane helix 2 from 176 (out) to 191 (in) : -583.8 Transmembrane helix 3 from 212 (in) to 227 (out) : -404.809 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 26 (out) to 41 (in) : -210.994 Transmembrane helix 2 from 212 (in) to 227 (out) : -404.809 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = -0.299825 1 helix (-) : Score = -0.130375 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 Results: Signal peptide: 1-22 Signal score: 66.204 Topology: 26-41 Re-entrant helices: Not detected. Helix count: 1 N-terminal: out Score: -0.130375