Processing 1 helix: Transmembrane helix 1 from 269 (in) to 295 (out) : 2770.8 Score = 2.770800 Processing 1 helix: Transmembrane helix 1 from 269 (out) to 295 (in) : 2770.8 Score = 2.770800 Processing 24 helices: Transmembrane helix 1 from 2 (in) to 1 (out) : -77.386 Transmembrane helix 2 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 3 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 4 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 5 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 6 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 7 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 8 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 9 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 10 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 11 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 12 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 13 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 14 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 15 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 16 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 17 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 18 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 19 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 20 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 21 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 22 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 23 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 24 from 1 (out) to 0 (in) : -8.429 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 1251.79 Transmembrane helix 2 from 34 (in) to 49 (out) : 122.04 Transmembrane helix 3 from 53 (out) to 70 (in) : 923.783 Transmembrane helix 4 from 74 (in) to 89 (out) : 217.686 Transmembrane helix 5 from 93 (out) to 108 (in) : 329.586 Transmembrane helix 6 from 112 (in) to 127 (out) : 589.347 Transmembrane helix 7 from 131 (out) to 146 (in) : -177.3 Transmembrane helix 8 from 150 (in) to 165 (out) : 350.6 Transmembrane helix 9 from 169 (out) to 184 (in) : 841.1 Transmembrane helix 10 from 188 (in) to 203 (out) : -936.75 Transmembrane helix 11 from 207 (out) to 222 (in) : 1945.7 Transmembrane helix 12 from 229 (in) to 244 (out) : 1593.35 Transmembrane helix 13 from 248 (out) to 263 (in) : -180.4 Transmembrane helix 14 from 267 (in) to 282 (out) : 1405.65 Transmembrane helix 15 from 286 (out) to 301 (in) : 964.95 Transmembrane helix 16 from 305 (in) to 320 (out) : 0 Transmembrane helix 17 from 324 (out) to 339 (in) : 0 Transmembrane helix 18 from 343 (in) to 358 (out) : 0 Transmembrane helix 19 from 362 (out) to 377 (in) : 0 Transmembrane helix 20 from 381 (in) to 396 (out) : 0 Transmembrane helix 21 from 400 (out) to 415 (in) : 0 Transmembrane helix 22 from 419 (in) to 434 (out) : 0 Transmembrane helix 23 from 438 (out) to 453 (in) : 0 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 1261.21 Transmembrane helix 2 from 34 (out) to 49 (in) : 120.76 Transmembrane helix 3 from 53 (in) to 71 (out) : 945.404 Transmembrane helix 4 from 75 (out) to 90 (in) : 286.612 Transmembrane helix 5 from 94 (in) to 109 (out) : 320.837 Transmembrane helix 6 from 113 (out) to 128 (in) : 562.429 Transmembrane helix 7 from 137 (in) to 152 (out) : 278.4 Transmembrane helix 8 from 156 (out) to 171 (in) : 179.15 Transmembrane helix 9 from 175 (in) to 190 (out) : 698.85 Transmembrane helix 10 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 11 from 229 (in) to 244 (out) : 1593.35 Transmembrane helix 12 from 248 (out) to 263 (in) : -180.4 Transmembrane helix 13 from 267 (in) to 282 (out) : 1405.65 Transmembrane helix 14 from 286 (out) to 301 (in) : 964.95 Transmembrane helix 15 from 305 (in) to 320 (out) : 0 Transmembrane helix 16 from 324 (out) to 339 (in) : 0 Transmembrane helix 17 from 343 (in) to 358 (out) : 0 Transmembrane helix 18 from 362 (out) to 377 (in) : 0 Transmembrane helix 19 from 381 (in) to 396 (out) : 0 Transmembrane helix 20 from 400 (out) to 415 (in) : 0 Transmembrane helix 21 from 419 (in) to 434 (out) : 0 Transmembrane helix 22 from 438 (out) to 453 (in) : 0 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 1251.79 Transmembrane helix 2 from 34 (in) to 49 (out) : 122.04 Transmembrane helix 3 from 53 (out) to 71 (in) : 954.996 Transmembrane helix 4 from 78 (in) to 93 (out) : 522.258 Transmembrane helix 5 from 97 (out) to 112 (in) : 241.655 Transmembrane helix 6 from 116 (in) to 131 (out) : 404.427 Transmembrane helix 7 from 137 (out) to 152 (in) : 278.4 Transmembrane helix 8 from 156 (in) to 171 (out) : 179.15 Transmembrane helix 9 from 175 (out) to 190 (in) : 698.85 Transmembrane helix 10 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 11 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 12 from 264 (in) to 279 (out) : 894.65 Transmembrane helix 13 from 283 (out) to 298 (in) : 1490.9 Transmembrane helix 14 from 304 (in) to 319 (out) : 0 Transmembrane helix 15 from 323 (out) to 338 (in) : 0 Transmembrane helix 16 from 342 (in) to 357 (out) : 0 Transmembrane helix 17 from 361 (out) to 376 (in) : 0 Transmembrane helix 18 from 380 (in) to 395 (out) : 0 Transmembrane helix 19 from 399 (out) to 414 (in) : 0 Transmembrane helix 20 from 418 (in) to 433 (out) : 0 Transmembrane helix 21 from 437 (out) to 452 (in) : 0 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 1261.21 Transmembrane helix 2 from 34 (out) to 49 (in) : 120.76 Transmembrane helix 3 from 53 (in) to 71 (out) : 945.404 Transmembrane helix 4 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 5 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 6 from 137 (out) to 152 (in) : 278.4 Transmembrane helix 7 from 156 (in) to 171 (out) : 179.15 Transmembrane helix 8 from 175 (out) to 190 (in) : 698.85 Transmembrane helix 9 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 10 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 11 from 264 (in) to 279 (out) : 894.65 Transmembrane helix 12 from 283 (out) to 298 (in) : 1490.9 Transmembrane helix 13 from 304 (in) to 319 (out) : 0 Transmembrane helix 14 from 323 (out) to 338 (in) : 0 Transmembrane helix 15 from 342 (in) to 357 (out) : 0 Transmembrane helix 16 from 361 (out) to 376 (in) : 0 Transmembrane helix 17 from 380 (in) to 395 (out) : 0 Transmembrane helix 18 from 399 (out) to 414 (in) : 0 Transmembrane helix 19 from 418 (in) to 433 (out) : 0 Transmembrane helix 20 from 437 (out) to 452 (in) : 0 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 1251.79 Transmembrane helix 2 from 34 (in) to 49 (out) : 122.04 Transmembrane helix 3 from 53 (out) to 71 (in) : 954.996 Transmembrane helix 4 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 5 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 6 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 7 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 8 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 9 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 10 from 264 (in) to 279 (out) : 894.65 Transmembrane helix 11 from 283 (out) to 298 (in) : 1490.9 Transmembrane helix 12 from 304 (in) to 319 (out) : 0 Transmembrane helix 13 from 323 (out) to 338 (in) : 0 Transmembrane helix 14 from 342 (in) to 357 (out) : 0 Transmembrane helix 15 from 361 (out) to 376 (in) : 0 Transmembrane helix 16 from 380 (in) to 395 (out) : 0 Transmembrane helix 17 from 399 (out) to 414 (in) : 0 Transmembrane helix 18 from 418 (in) to 433 (out) : 0 Transmembrane helix 19 from 437 (out) to 452 (in) : 0 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 1261.21 Transmembrane helix 2 from 34 (out) to 49 (in) : 120.76 Transmembrane helix 3 from 53 (in) to 71 (out) : 945.404 Transmembrane helix 4 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 5 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 6 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 7 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 8 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 9 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 10 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 11 from 304 (in) to 319 (out) : 0 Transmembrane helix 12 from 323 (out) to 338 (in) : 0 Transmembrane helix 13 from 342 (in) to 357 (out) : 0 Transmembrane helix 14 from 361 (out) to 376 (in) : 0 Transmembrane helix 15 from 380 (in) to 395 (out) : 0 Transmembrane helix 16 from 399 (out) to 414 (in) : 0 Transmembrane helix 17 from 418 (in) to 433 (out) : 0 Transmembrane helix 18 from 437 (out) to 452 (in) : 0 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Transmembrane helix 12 from 342 (in) to 357 (out) : 0 Transmembrane helix 13 from 361 (out) to 376 (in) : 0 Transmembrane helix 14 from 380 (in) to 395 (out) : 0 Transmembrane helix 15 from 399 (out) to 414 (in) : 0 Transmembrane helix 16 from 418 (in) to 433 (out) : 0 Transmembrane helix 17 from 437 (out) to 452 (in) : 0 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Transmembrane helix 12 from 342 (out) to 357 (in) : 0 Transmembrane helix 13 from 361 (in) to 376 (out) : 0 Transmembrane helix 14 from 380 (out) to 395 (in) : 0 Transmembrane helix 15 from 399 (in) to 414 (out) : 0 Transmembrane helix 16 from 418 (out) to 433 (in) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Transmembrane helix 12 from 342 (in) to 357 (out) : 0 Transmembrane helix 13 from 361 (out) to 376 (in) : 0 Transmembrane helix 14 from 380 (in) to 395 (out) : 0 Transmembrane helix 15 from 399 (out) to 414 (in) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Transmembrane helix 12 from 342 (out) to 357 (in) : 0 Transmembrane helix 13 from 361 (in) to 376 (out) : 0 Transmembrane helix 14 from 380 (out) to 395 (in) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Transmembrane helix 12 from 342 (in) to 357 (out) : 0 Transmembrane helix 13 from 361 (out) to 376 (in) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Transmembrane helix 12 from 342 (out) to 357 (in) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Score = 12.658088 Processing 8 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 154 (in) to 184 (out) : 1041.95 Transmembrane helix 6 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 7 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 8 from 269 (out) to 295 (in) : 2770.8 Score = 12.333832 Processing 7 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 89 (out) to 119 (in) : 977.07 Transmembrane helix 4 from 154 (in) to 184 (out) : 1041.95 Transmembrane helix 5 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 6 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 7 from 269 (out) to 295 (in) : 2770.8 Score = 11.524545 Processing 6 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 60 (out) to 90 (in) : 984.399 Transmembrane helix 3 from 154 (in) to 184 (out) : 1041.95 Transmembrane helix 4 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 5 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 6 from 269 (out) to 295 (in) : 2770.8 Score = 10.720761 Processing 5 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 1599.49 Transmembrane helix 2 from 154 (in) to 184 (out) : 1041.95 Transmembrane helix 3 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 4 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 5 from 269 (out) to 295 (in) : 2770.8 Score = 9.631414 Processing 4 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 3 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 4 from 269 (out) to 295 (in) : 2770.8 Score = 8.669537 Processing 3 helices: Transmembrane helix 1 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 2 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 3 from 269 (out) to 295 (in) : 2770.8 Score = 7.010300 Processing 2 helices: Transmembrane helix 1 from 213 (in) to 243 (out) : 2287.7 Transmembrane helix 2 from 269 (out) to 295 (in) : 2770.8 Score = 5.058500 Processing 24 helices: Transmembrane helix 1 from 2 (out) to 1 (in) : -91.191 Transmembrane helix 2 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 3 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 4 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 5 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 6 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 7 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 8 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 9 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 10 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 11 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 12 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 13 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 14 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 15 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 16 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 17 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 18 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 19 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 20 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 21 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 22 from 1 (in) to 0 (out) : 7.549 Transmembrane helix 23 from 1 (out) to 0 (in) : -8.429 Transmembrane helix 24 from 1 (in) to 0 (out) : 7.549 Score = -100000.000000 Processing 23 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 1261.21 Transmembrane helix 2 from 34 (out) to 49 (in) : 120.76 Transmembrane helix 3 from 53 (in) to 70 (out) : 915.217 Transmembrane helix 4 from 74 (out) to 89 (in) : 208.414 Transmembrane helix 5 from 93 (in) to 108 (out) : 340.614 Transmembrane helix 6 from 112 (out) to 127 (in) : 600.853 Transmembrane helix 7 from 131 (in) to 146 (out) : -177.3 Transmembrane helix 8 from 150 (out) to 165 (in) : 350.6 Transmembrane helix 9 from 169 (in) to 184 (out) : 841.1 Transmembrane helix 10 from 188 (out) to 203 (in) : -936.75 Transmembrane helix 11 from 207 (in) to 222 (out) : 1945.7 Transmembrane helix 12 from 229 (out) to 244 (in) : 1593.35 Transmembrane helix 13 from 248 (in) to 263 (out) : -180.4 Transmembrane helix 14 from 267 (out) to 282 (in) : 1405.65 Transmembrane helix 15 from 286 (in) to 301 (out) : 964.95 Transmembrane helix 16 from 305 (out) to 320 (in) : 0 Transmembrane helix 17 from 324 (in) to 339 (out) : 0 Transmembrane helix 18 from 343 (out) to 358 (in) : 0 Transmembrane helix 19 from 362 (in) to 377 (out) : 0 Transmembrane helix 20 from 381 (out) to 396 (in) : 0 Transmembrane helix 21 from 400 (in) to 415 (out) : 0 Transmembrane helix 22 from 419 (out) to 434 (in) : 0 Transmembrane helix 23 from 438 (in) to 453 (out) : 0 Score = -100000.000000 Processing 22 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 1251.79 Transmembrane helix 2 from 34 (in) to 49 (out) : 122.04 Transmembrane helix 3 from 53 (out) to 71 (in) : 954.996 Transmembrane helix 4 from 78 (in) to 93 (out) : 522.258 Transmembrane helix 5 from 97 (out) to 112 (in) : 241.655 Transmembrane helix 6 from 116 (in) to 131 (out) : 404.427 Transmembrane helix 7 from 137 (out) to 152 (in) : 278.4 Transmembrane helix 8 from 156 (in) to 171 (out) : 179.15 Transmembrane helix 9 from 175 (out) to 190 (in) : 698.85 Transmembrane helix 10 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 11 from 229 (out) to 244 (in) : 1593.35 Transmembrane helix 12 from 248 (in) to 263 (out) : -180.4 Transmembrane helix 13 from 267 (out) to 282 (in) : 1405.65 Transmembrane helix 14 from 286 (in) to 301 (out) : 964.95 Transmembrane helix 15 from 305 (out) to 320 (in) : 0 Transmembrane helix 16 from 324 (in) to 339 (out) : 0 Transmembrane helix 17 from 343 (out) to 358 (in) : 0 Transmembrane helix 18 from 362 (in) to 377 (out) : 0 Transmembrane helix 19 from 381 (out) to 396 (in) : 0 Transmembrane helix 20 from 400 (in) to 415 (out) : 0 Transmembrane helix 21 from 419 (out) to 434 (in) : 0 Transmembrane helix 22 from 438 (in) to 453 (out) : 0 Score = -100000.000000 Processing 21 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 1261.21 Transmembrane helix 2 from 34 (out) to 49 (in) : 120.76 Transmembrane helix 3 from 53 (in) to 71 (out) : 945.404 Transmembrane helix 4 from 75 (out) to 90 (in) : 286.612 Transmembrane helix 5 from 94 (in) to 109 (out) : 320.837 Transmembrane helix 6 from 113 (out) to 128 (in) : 562.429 Transmembrane helix 7 from 137 (in) to 152 (out) : 278.4 Transmembrane helix 8 from 156 (out) to 171 (in) : 179.15 Transmembrane helix 9 from 175 (in) to 190 (out) : 698.85 Transmembrane helix 10 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 11 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 12 from 264 (out) to 279 (in) : 894.65 Transmembrane helix 13 from 283 (in) to 298 (out) : 1490.9 Transmembrane helix 14 from 304 (out) to 319 (in) : 0 Transmembrane helix 15 from 323 (in) to 338 (out) : 0 Transmembrane helix 16 from 342 (out) to 357 (in) : 0 Transmembrane helix 17 from 361 (in) to 376 (out) : 0 Transmembrane helix 18 from 380 (out) to 395 (in) : 0 Transmembrane helix 19 from 399 (in) to 414 (out) : 0 Transmembrane helix 20 from 418 (out) to 433 (in) : 0 Transmembrane helix 21 from 437 (in) to 452 (out) : 0 Score = -100000.000000 Processing 20 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 1251.79 Transmembrane helix 2 from 34 (in) to 49 (out) : 122.04 Transmembrane helix 3 from 53 (out) to 71 (in) : 954.996 Transmembrane helix 4 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 5 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 6 from 137 (in) to 152 (out) : 278.4 Transmembrane helix 7 from 156 (out) to 171 (in) : 179.15 Transmembrane helix 8 from 175 (in) to 190 (out) : 698.85 Transmembrane helix 9 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 10 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 11 from 264 (out) to 279 (in) : 894.65 Transmembrane helix 12 from 283 (in) to 298 (out) : 1490.9 Transmembrane helix 13 from 304 (out) to 319 (in) : 0 Transmembrane helix 14 from 323 (in) to 338 (out) : 0 Transmembrane helix 15 from 342 (out) to 357 (in) : 0 Transmembrane helix 16 from 361 (in) to 376 (out) : 0 Transmembrane helix 17 from 380 (out) to 395 (in) : 0 Transmembrane helix 18 from 399 (in) to 414 (out) : 0 Transmembrane helix 19 from 418 (out) to 433 (in) : 0 Transmembrane helix 20 from 437 (in) to 452 (out) : 0 Score = -100000.000000 Processing 19 helices: Transmembrane helix 1 from 15 (in) to 30 (out) : 1261.21 Transmembrane helix 2 from 34 (out) to 49 (in) : 120.76 Transmembrane helix 3 from 53 (in) to 71 (out) : 945.404 Transmembrane helix 4 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 5 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 6 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 7 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 8 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 9 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 10 from 264 (out) to 279 (in) : 894.65 Transmembrane helix 11 from 283 (in) to 298 (out) : 1490.9 Transmembrane helix 12 from 304 (out) to 319 (in) : 0 Transmembrane helix 13 from 323 (in) to 338 (out) : 0 Transmembrane helix 14 from 342 (out) to 357 (in) : 0 Transmembrane helix 15 from 361 (in) to 376 (out) : 0 Transmembrane helix 16 from 380 (out) to 395 (in) : 0 Transmembrane helix 17 from 399 (in) to 414 (out) : 0 Transmembrane helix 18 from 418 (out) to 433 (in) : 0 Transmembrane helix 19 from 437 (in) to 452 (out) : 0 Score = -100000.000000 Processing 18 helices: Transmembrane helix 1 from 15 (out) to 30 (in) : 1251.79 Transmembrane helix 2 from 34 (in) to 49 (out) : 122.04 Transmembrane helix 3 from 53 (out) to 71 (in) : 954.996 Transmembrane helix 4 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 5 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 6 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 7 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 8 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 9 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 10 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 11 from 304 (out) to 319 (in) : 0 Transmembrane helix 12 from 323 (in) to 338 (out) : 0 Transmembrane helix 13 from 342 (out) to 357 (in) : 0 Transmembrane helix 14 from 361 (in) to 376 (out) : 0 Transmembrane helix 15 from 380 (out) to 395 (in) : 0 Transmembrane helix 16 from 399 (in) to 414 (out) : 0 Transmembrane helix 17 from 418 (out) to 433 (in) : 0 Transmembrane helix 18 from 437 (in) to 452 (out) : 0 Score = -100000.000000 Processing 17 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Transmembrane helix 12 from 342 (out) to 357 (in) : 0 Transmembrane helix 13 from 361 (in) to 376 (out) : 0 Transmembrane helix 14 from 380 (out) to 395 (in) : 0 Transmembrane helix 15 from 399 (in) to 414 (out) : 0 Transmembrane helix 16 from 418 (out) to 433 (in) : 0 Transmembrane helix 17 from 437 (in) to 452 (out) : 0 Score = -100000.000000 Processing 16 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Transmembrane helix 12 from 342 (in) to 357 (out) : 0 Transmembrane helix 13 from 361 (out) to 376 (in) : 0 Transmembrane helix 14 from 380 (in) to 395 (out) : 0 Transmembrane helix 15 from 399 (out) to 414 (in) : 0 Transmembrane helix 16 from 418 (in) to 433 (out) : 0 Score = -100000.000000 Processing 15 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Transmembrane helix 12 from 342 (out) to 357 (in) : 0 Transmembrane helix 13 from 361 (in) to 376 (out) : 0 Transmembrane helix 14 from 380 (out) to 395 (in) : 0 Transmembrane helix 15 from 399 (in) to 414 (out) : 0 Score = -100000.000000 Processing 14 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Transmembrane helix 12 from 342 (in) to 357 (out) : 0 Transmembrane helix 13 from 361 (out) to 376 (in) : 0 Transmembrane helix 14 from 380 (in) to 395 (out) : 0 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Transmembrane helix 12 from 342 (out) to 357 (in) : 0 Transmembrane helix 13 from 361 (in) to 376 (out) : 0 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Transmembrane helix 11 from 323 (out) to 338 (in) : 0 Transmembrane helix 12 from 342 (in) to 357 (out) : 0 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Transmembrane helix 10 from 304 (out) to 319 (in) : 0 Transmembrane helix 11 from 323 (in) to 338 (out) : 0 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 142 (out) to 162 (in) : 682.3 Transmembrane helix 6 from 168 (in) to 186 (out) : 876.4 Transmembrane helix 7 from 206 (out) to 222 (in) : 1997.8 Transmembrane helix 8 from 234 (in) to 250 (out) : 2241.7 Transmembrane helix 9 from 269 (out) to 295 (in) : 2770.8 Transmembrane helix 10 from 304 (in) to 319 (out) : 0 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 81 (in) to 100 (out) : 910.441 Transmembrane helix 4 from 106 (out) to 126 (in) : 720.47 Transmembrane helix 5 from 142 (in) to 162 (out) : 682.3 Transmembrane helix 6 from 168 (out) to 186 (in) : 876.4 Transmembrane helix 7 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 8 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 9 from 269 (in) to 295 (out) : 2770.8 Score = 12.850582 Processing 8 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 52 (in) to 71 (out) : 946.623 Transmembrane helix 3 from 81 (out) to 100 (in) : 894.159 Transmembrane helix 4 from 106 (in) to 126 (out) : 700.13 Transmembrane helix 5 from 154 (out) to 184 (in) : 1041.95 Transmembrane helix 6 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 7 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 8 from 269 (in) to 295 (out) : 2770.8 Score = 12.141338 Processing 7 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 52 (out) to 71 (in) : 959.377 Transmembrane helix 3 from 89 (in) to 119 (out) : 983.73 Transmembrane helix 4 from 154 (out) to 184 (in) : 1041.95 Transmembrane helix 5 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 6 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 7 from 269 (in) to 295 (out) : 2770.8 Score = 11.686226 Processing 6 helices: Transmembrane helix 1 from 16 (out) to 41 (in) : 1597.56 Transmembrane helix 2 from 89 (in) to 119 (out) : 983.73 Transmembrane helix 3 from 154 (out) to 184 (in) : 1041.95 Transmembrane helix 4 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 5 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 6 from 269 (in) to 295 (out) : 2770.8 Score = 10.602423 Processing 5 helices: Transmembrane helix 1 from 16 (in) to 39 (out) : 1638.91 Transmembrane helix 2 from 154 (out) to 184 (in) : 1041.95 Transmembrane helix 3 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 4 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 5 from 269 (in) to 295 (out) : 2770.8 Score = 9.711486 Processing 4 helices: Transmembrane helix 1 from 16 (out) to 39 (in) : 1599.49 Transmembrane helix 2 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 3 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 4 from 269 (in) to 295 (out) : 2770.8 Score = 8.589464 Processing 3 helices: Transmembrane helix 1 from 206 (in) to 222 (out) : 1997.8 Transmembrane helix 2 from 234 (out) to 250 (in) : 2241.7 Transmembrane helix 3 from 269 (in) to 295 (out) : 2770.8 Score = 7.010300 Processing 2 helices: Transmembrane helix 1 from 213 (out) to 243 (in) : 2287.7 Transmembrane helix 2 from 269 (in) to 295 (out) : 2770.8 Score = 5.058500 Summary of topology analysis: 1 helix (+) : Score = 2.7708 1 helix (-) : Score = 2.7708 2 helices (+) : Score = 5.0585 2 helices (-) : Score = 5.0585 3 helices (+) : Score = 7.0103 3 helices (-) : Score = 7.0103 4 helices (+) : Score = 8.66954 4 helices (-) : Score = 8.58946 5 helices (+) : Score = 9.71149 5 helices (-) : Score = 9.63141 6 helices (+) : Score = 10.7208 6 helices (-) : Score = 10.6024 7 helices (+) : Score = 11.6862 7 helices (-) : Score = 11.5245 8 helices (+) : Score = 12.3338 8 helices (-) : Score = 12.1413 9 helices (+) : Score = 12.8506 9 helices (-) : Score = 12.6581 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 14 helices (+) : Score = -100000 14 helices (-) : Score = -100000 15 helices (+) : Score = -100000 15 helices (-) : Score = -100000 16 helices (+) : Score = -100000 16 helices (-) : Score = -100000 17 helices (+) : Score = -100000 17 helices (-) : Score = -100000 18 helices (+) : Score = -100000 18 helices (-) : Score = -100000 19 helices (+) : Score = -100000 19 helices (-) : Score = -100000 20 helices (+) : Score = -100000 20 helices (-) : Score = -100000 21 helices (+) : Score = -100000 21 helices (-) : Score = -100000 22 helices (+) : Score = -100000 22 helices (-) : Score = -100000 23 helices (+) : Score = -100000 23 helices (-) : Score = -100000 24 helices (+) : Score = -100000 24 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 1.082 Topology: 16-39,52-71,81-100,106-126,142-162,168-186,206-222,234-250,269-295 Re-entrant helices: Not detected. Helix count: 9 N-terminal: in Score: 12.8506