Processing 1 helix: Transmembrane helix 1 from 42 (in) to 66 (out) : 2965.86 Score = 2.919602 Processing 1 helix: Transmembrane helix 1 from 42 (out) to 67 (in) : 2983.88 Score = 3.030264 Processing 5 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : 457.935 Transmembrane helix 2 from 36 (out) to 51 (in) : -623.108 Transmembrane helix 3 from 55 (in) to 70 (out) : 1152.08 Transmembrane helix 4 from 74 (out) to 89 (in) : -1308.3 Transmembrane helix 5 from 93 (in) to 108 (out) : -1245.86 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : 453.065 Transmembrane helix 2 from 42 (in) to 57 (out) : 2346.83 Transmembrane helix 3 from 61 (out) to 76 (in) : -5.31204 Transmembrane helix 4 from 93 (in) to 108 (out) : -1245.86 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : 457.935 Transmembrane helix 2 from 42 (out) to 57 (in) : 2351.17 Transmembrane helix 3 from 61 (in) to 76 (out) : -15.088 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : 453.065 Transmembrane helix 2 from 42 (in) to 66 (out) : 2965.86 Score = 3.390196 Processing 5 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : 453.065 Transmembrane helix 2 from 36 (in) to 51 (out) : -631.392 Transmembrane helix 3 from 55 (out) to 70 (in) : 1167.62 Transmembrane helix 4 from 74 (in) to 89 (out) : -1312.8 Transmembrane helix 5 from 93 (out) to 108 (in) : -1252.64 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : 457.935 Transmembrane helix 2 from 42 (out) to 57 (in) : 2351.17 Transmembrane helix 3 from 61 (in) to 76 (out) : -15.088 Transmembrane helix 4 from 93 (out) to 108 (in) : -1252.64 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 10 (out) to 25 (in) : 453.065 Transmembrane helix 2 from 42 (in) to 57 (out) : 2346.83 Transmembrane helix 3 from 61 (out) to 76 (in) : -5.31204 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 10 (in) to 25 (out) : 457.935 Transmembrane helix 2 from 42 (out) to 67 (in) : 2983.88 Score = 3.470670 Summary of topology analysis: 1 helix (+) : Score = 2.9196 1 helix (-) : Score = 3.03026 2 helices (+) : Score = 3.47067 2 helices (-) : Score = 3.3902 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 11.568 Topology: 10-25,42-67 Re-entrant helices: Not detected. Helix count: 2 N-terminal: out Score: 3.47067