Processing 1 helix: Transmembrane helix 1 from 64 (in) to 79 (out) : 15.032 Score = 0.015032 Processing 1 helix: Transmembrane helix 1 from 104 (out) to 119 (in) : 13.791 Score = 0.013791 Processing 13 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 42 (in) to 57 (out) : -985.62 Transmembrane helix 4 from 61 (out) to 76 (in) : -33.069 Transmembrane helix 5 from 80 (in) to 95 (out) : -334.641 Transmembrane helix 6 from 99 (out) to 114 (in) : -83.453 Transmembrane helix 7 from 118 (in) to 133 (out) : -758.787 Transmembrane helix 8 from 137 (out) to 152 (in) : -243.923 Transmembrane helix 9 from 156 (in) to 171 (out) : -674.23 Transmembrane helix 10 from 175 (out) to 190 (in) : -1097.82 Transmembrane helix 11 from 196 (in) to 211 (out) : -1180.62 Transmembrane helix 12 from 215 (out) to 230 (in) : -280.772 Transmembrane helix 13 from 234 (in) to 249 (out) : -593.492 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -368.646 Transmembrane helix 2 from 23 (in) to 38 (out) : -524.288 Transmembrane helix 3 from 50 (out) to 65 (in) : -695.383 Transmembrane helix 4 from 69 (in) to 84 (out) : -68.492 Transmembrane helix 5 from 88 (out) to 103 (in) : -358.934 Transmembrane helix 6 from 107 (in) to 122 (out) : -60.501 Transmembrane helix 7 from 131 (out) to 146 (in) : -287.389 Transmembrane helix 8 from 150 (in) to 165 (out) : -633.639 Transmembrane helix 9 from 169 (out) to 184 (in) : -840.893 Transmembrane helix 10 from 196 (in) to 211 (out) : -1180.62 Transmembrane helix 11 from 215 (out) to 230 (in) : -280.772 Transmembrane helix 12 from 234 (in) to 249 (out) : -593.492 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 51 (in) to 66 (out) : -646.758 Transmembrane helix 4 from 70 (out) to 85 (in) : -97.476 Transmembrane helix 5 from 89 (in) to 104 (out) : -377.25 Transmembrane helix 6 from 108 (out) to 123 (in) : -88.335 Transmembrane helix 7 from 131 (in) to 146 (out) : -281.211 Transmembrane helix 8 from 150 (out) to 165 (in) : -637.561 Transmembrane helix 9 from 169 (in) to 184 (out) : -843.807 Transmembrane helix 10 from 213 (out) to 228 (in) : -304.757 Transmembrane helix 11 from 232 (in) to 247 (out) : -562.915 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -368.646 Transmembrane helix 2 from 23 (in) to 38 (out) : -524.288 Transmembrane helix 3 from 50 (out) to 65 (in) : -695.383 Transmembrane helix 4 from 69 (in) to 84 (out) : -68.492 Transmembrane helix 5 from 88 (out) to 103 (in) : -358.934 Transmembrane helix 6 from 107 (in) to 122 (out) : -60.501 Transmembrane helix 7 from 133 (out) to 148 (in) : -228.866 Transmembrane helix 8 from 152 (in) to 167 (out) : -651.662 Transmembrane helix 9 from 213 (out) to 228 (in) : -304.757 Transmembrane helix 10 from 232 (in) to 247 (out) : -562.915 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 4 from 84 (out) to 99 (in) : -354.592 Transmembrane helix 5 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 6 from 133 (out) to 148 (in) : -228.866 Transmembrane helix 7 from 152 (in) to 167 (out) : -651.662 Transmembrane helix 8 from 213 (out) to 228 (in) : -304.757 Transmembrane helix 9 from 232 (in) to 247 (out) : -562.915 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -368.646 Transmembrane helix 2 from 23 (in) to 38 (out) : -524.288 Transmembrane helix 3 from 62 (out) to 77 (in) : -11.686 Transmembrane helix 4 from 81 (in) to 96 (out) : -356.306 Transmembrane helix 5 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 6 from 134 (in) to 149 (out) : -214.105 Transmembrane helix 7 from 213 (out) to 228 (in) : -304.757 Transmembrane helix 8 from 232 (in) to 247 (out) : -562.915 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 4 from 84 (out) to 99 (in) : -354.592 Transmembrane helix 5 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 6 from 134 (out) to 149 (in) : -217.595 Transmembrane helix 7 from 216 (in) to 231 (out) : -273.512 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -298.794 Transmembrane helix 2 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 3 from 84 (out) to 99 (in) : -354.592 Transmembrane helix 4 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 5 from 134 (out) to 149 (in) : -217.595 Transmembrane helix 6 from 216 (in) to 231 (out) : -273.512 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -302.606 Transmembrane helix 2 from 64 (out) to 79 (in) : -0.131998 Transmembrane helix 3 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 4 from 134 (out) to 149 (in) : -217.595 Transmembrane helix 5 from 216 (in) to 231 (out) : -273.512 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -298.794 Transmembrane helix 2 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 3 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 4 from 134 (in) to 149 (out) : -214.105 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 2 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 3 from 134 (in) to 149 (out) : -214.105 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 64 (out) to 79 (in) : -0.131998 Transmembrane helix 2 from 104 (in) to 119 (out) : 13.809 Score = -100000.000000 Processing 13 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -368.646 Transmembrane helix 2 from 23 (in) to 38 (out) : -524.288 Transmembrane helix 3 from 42 (out) to 57 (in) : -1003.58 Transmembrane helix 4 from 61 (in) to 76 (out) : -22.231 Transmembrane helix 5 from 80 (out) to 95 (in) : -332.059 Transmembrane helix 6 from 99 (in) to 114 (out) : -74.547 Transmembrane helix 7 from 118 (out) to 133 (in) : -754.513 Transmembrane helix 8 from 137 (in) to 152 (out) : -240.177 Transmembrane helix 9 from 156 (out) to 171 (in) : -680.57 Transmembrane helix 10 from 175 (in) to 190 (out) : -1099.28 Transmembrane helix 11 from 196 (out) to 211 (in) : -1188.88 Transmembrane helix 12 from 215 (in) to 230 (out) : -282.428 Transmembrane helix 13 from 234 (out) to 249 (in) : -613.708 Score = -100000.000000 Processing 12 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 51 (in) to 66 (out) : -646.758 Transmembrane helix 4 from 70 (out) to 85 (in) : -97.476 Transmembrane helix 5 from 89 (in) to 104 (out) : -377.25 Transmembrane helix 6 from 108 (out) to 123 (in) : -88.335 Transmembrane helix 7 from 131 (in) to 146 (out) : -281.211 Transmembrane helix 8 from 150 (out) to 165 (in) : -637.561 Transmembrane helix 9 from 169 (in) to 184 (out) : -843.807 Transmembrane helix 10 from 196 (out) to 211 (in) : -1188.88 Transmembrane helix 11 from 215 (in) to 230 (out) : -282.428 Transmembrane helix 12 from 234 (out) to 249 (in) : -613.708 Score = -100000.000000 Processing 11 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -368.646 Transmembrane helix 2 from 23 (in) to 38 (out) : -524.288 Transmembrane helix 3 from 50 (out) to 65 (in) : -695.383 Transmembrane helix 4 from 69 (in) to 84 (out) : -68.492 Transmembrane helix 5 from 88 (out) to 103 (in) : -358.934 Transmembrane helix 6 from 107 (in) to 122 (out) : -60.501 Transmembrane helix 7 from 131 (out) to 146 (in) : -287.389 Transmembrane helix 8 from 150 (in) to 165 (out) : -633.639 Transmembrane helix 9 from 169 (out) to 184 (in) : -840.893 Transmembrane helix 10 from 213 (in) to 228 (out) : -310.743 Transmembrane helix 11 from 232 (out) to 247 (in) : -581.185 Score = -100000.000000 Processing 10 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 4 from 84 (out) to 99 (in) : -354.592 Transmembrane helix 5 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 6 from 131 (out) to 146 (in) : -287.389 Transmembrane helix 7 from 150 (in) to 165 (out) : -633.639 Transmembrane helix 8 from 169 (out) to 184 (in) : -840.893 Transmembrane helix 9 from 213 (in) to 228 (out) : -310.743 Transmembrane helix 10 from 232 (out) to 247 (in) : -581.185 Score = -100000.000000 Processing 9 helices: Transmembrane helix 1 from 4 (out) to 19 (in) : -368.646 Transmembrane helix 2 from 23 (in) to 38 (out) : -524.288 Transmembrane helix 3 from 62 (out) to 77 (in) : -11.686 Transmembrane helix 4 from 81 (in) to 96 (out) : -356.306 Transmembrane helix 5 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 6 from 133 (in) to 148 (out) : -224.734 Transmembrane helix 7 from 152 (out) to 167 (in) : -659.038 Transmembrane helix 8 from 213 (in) to 228 (out) : -310.743 Transmembrane helix 9 from 232 (out) to 247 (in) : -581.185 Score = -100000.000000 Processing 8 helices: Transmembrane helix 1 from 4 (in) to 19 (out) : -371.754 Transmembrane helix 2 from 23 (out) to 38 (in) : -517.212 Transmembrane helix 3 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 4 from 84 (out) to 99 (in) : -354.592 Transmembrane helix 5 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 6 from 134 (out) to 149 (in) : -217.595 Transmembrane helix 7 from 213 (in) to 228 (out) : -310.743 Transmembrane helix 8 from 232 (out) to 247 (in) : -581.185 Score = -100000.000000 Processing 7 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -298.794 Transmembrane helix 2 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 3 from 84 (out) to 99 (in) : -354.592 Transmembrane helix 4 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 5 from 134 (out) to 149 (in) : -217.595 Transmembrane helix 6 from 213 (in) to 228 (out) : -310.743 Transmembrane helix 7 from 232 (out) to 247 (in) : -581.185 Score = -100000.000000 Processing 6 helices: Transmembrane helix 1 from 13 (in) to 28 (out) : -302.606 Transmembrane helix 2 from 62 (out) to 77 (in) : -11.686 Transmembrane helix 3 from 81 (in) to 96 (out) : -356.306 Transmembrane helix 4 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 5 from 134 (in) to 149 (out) : -214.105 Transmembrane helix 6 from 216 (out) to 231 (in) : -275.588 Score = -100000.000000 Processing 5 helices: Transmembrane helix 1 from 13 (out) to 28 (in) : -298.794 Transmembrane helix 2 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 3 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 4 from 134 (in) to 149 (out) : -214.105 Transmembrane helix 5 from 216 (out) to 231 (in) : -275.588 Score = -100000.000000 Processing 4 helices: Transmembrane helix 1 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 2 from 104 (out) to 119 (in) : 13.791 Transmembrane helix 3 from 134 (in) to 149 (out) : -214.105 Transmembrane helix 4 from 216 (out) to 231 (in) : -275.588 Score = -100000.000000 Processing 3 helices: Transmembrane helix 1 from 64 (out) to 79 (in) : -0.131998 Transmembrane helix 2 from 104 (in) to 119 (out) : 13.809 Transmembrane helix 3 from 134 (out) to 149 (in) : -217.595 Score = -100000.000000 Processing 2 helices: Transmembrane helix 1 from 64 (in) to 79 (out) : 15.032 Transmembrane helix 2 from 104 (out) to 119 (in) : 13.791 Score = -100000.000000 Summary of topology analysis: 1 helix (+) : Score = 0.015032 1 helix (-) : Score = 0.013791 2 helices (+) : Score = -100000 2 helices (-) : Score = -100000 3 helices (+) : Score = -100000 3 helices (-) : Score = -100000 4 helices (+) : Score = -100000 4 helices (-) : Score = -100000 5 helices (+) : Score = -100000 5 helices (-) : Score = -100000 6 helices (+) : Score = -100000 6 helices (-) : Score = -100000 7 helices (+) : Score = -100000 7 helices (-) : Score = -100000 8 helices (+) : Score = -100000 8 helices (-) : Score = -100000 9 helices (+) : Score = -100000 9 helices (-) : Score = -100000 10 helices (+) : Score = -100000 10 helices (-) : Score = -100000 11 helices (+) : Score = -100000 11 helices (-) : Score = -100000 12 helices (+) : Score = -100000 12 helices (-) : Score = -100000 13 helices (+) : Score = -100000 13 helices (-) : Score = -100000 Results: Signal peptide: Not detected. Signal score: 0 Topology: 64-79 Re-entrant helices: Not detected. Helix count: 1 N-terminal: in Score: 0.015032